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MKDNTIKTETTVVGLLRKCVDEHPDTLFLDLAGDTYSYADVWQRSCDLAGMLHAGGVGAGQTVVSLQDTQ IDAIASWFGANMLGAIWVGTNTALRGEFLRHVITDTGARVVVAEADLVDRIRAIGQHLPDVDLVLQRGDE RPAADPHLRIERLVDLGGHTVNEPGDGAVDDLTCLTYTGGTTGPSKGCMISNGYAINIARRGLAQTQRQT DELNWSPLPMFHLNVLAMTLIGTMLVGGSAALAQRFSASRFWPEIERTGARVVNLIGGLPAILAQQPDTL EMARCFGQIRMVHAVPFPPALQEIWRNRFGVKVPGSKGYGMTEVFPITYQTPDEQSPAESAGRINHEDLE VRIVDERDDDVKPGEVGEVICRPRRSHVMFQGYWRRPDAMVDSCRNLWFHTGDLGRIDDAGYFYFADRKH DYLRRRGENISSQELETTYVAHPDIVAVAVHAVPSELTEDDVKVTVVLRPGSVLTPAELFEWSKERVPYF ALPRYVEFRTELPTSPLGRVHKYLLREQGCTPETWDREAAGVSWDRR
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. vanbaalenii PYR-1 | Mvan_0898 | - | - | 100% (537) | AMP-dependent synthetase and ligase |
| M. vanbaalenii PYR-1 | Mvan_0943 | - | 8e-48 | 31.35% (504) | AMP-dependent synthetase and ligase |
| M. vanbaalenii PYR-1 | Mvan_5209 | - | 2e-41 | 29.89% (455) | acyl-CoA synthetase |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb0172 | fadD5 | 2e-42 | 29.23% (520) | acyl-CoA synthetase |
| M. gilvum PYR-GCK | Mflv_0027 | - | 6e-47 | 31.56% (507) | AMP-dependent synthetase and ligase |
| M. tuberculosis H37Rv | Rv0166 | fadD5 | 5e-42 | 29.23% (520) | acyl-CoA synthetase |
| M. leprae Br4923 | MLBr_02661 | - | 5e-28 | 23.54% (497) | acyl-CoA synthetase |
| M. abscessus ATCC 19977 | MAB_4559 | - | 1e-42 | 30.02% (533) | putative long-chain-fatty-acid CoA ligase |
| M. marinum M | MMAR_1606 | - | 7e-42 | 27.49% (513) | fatty-acid-CoA ligase |
| M. avium 104 | MAV_1639 | - | 7e-82 | 36.08% (521) | AMP-binding enzyme, putative |
| M. smegmatis MC2 155 | MSMEG_4036 | - | 3e-51 | 30.42% (526) | crotonobetaine/carnitine-CoA ligase |
| M. thermoresistible (build 8) | TH_2476 | - | 9e-91 | 37.88% (520) | PUTATIVE putative AMP-dependent synthetase and ligase |
| M. ulcerans Agy99 | MUL_0229 | fadD14 | 6e-34 | 26.07% (537) | acyl-CoA synthetase |
CLUSTAL 2.0.9 multiple sequence alignment
Mb0172|M.bovis_AF2122/97 -MTAQLASHLTRALTLAQQQPYLARRQNWVNQLERHAMMQPDAPALRFVG
Rv0166|M.tuberculosis_H37Rv -MTAQLASHLTRALTLAQQQPYLARRQNWVNQLERHAMMQPDAPALRFVG
Mflv_0027|M.gilvum_PYR-GCK ------------------------MFHNLADIVRSHGRNRPDAPALIVGD
MAB_4559|M.abscessus_ATCC_1997 --------------------------MQFTQWLHRALQQYPDRLITIDQG
MMAR_1606|M.marinum_M -------------------------MTSLAGNLIEGARRHPNRIALRLDE
Mvan_0898|M.vanbaalenii_PYR-1 -----------------MKDNTIKTETTVVGLLRKCVDEHPDTLFLDLAG
TH_2476|M.thermoresistible__bu ---------------MHWIEDSSRENVAAGRALALQADAIGDAPFLASGD
MAV_1639|M.avium_104 ---------------MRAPADFGVDRFTVPAVLDRRAEQHPDRVMMSIAG
MSMEG_4036|M.smegmatis_MC2_155 -----------------------------MSYLSRAAARWPDRTWCITPR
MUL_0229|M.ulcerans_Agy99 -----------------MYSTMQDFPLTITAIMRHGCGVHGSRQVITATG
MLBr_02661|M.leprae_Br4923 MKSESITSSRAPVEQIDARLTGQFESPSIASLVEAAAIRNPSAPALVVTD
: .
Mb0172|M.bovis_AF2122/97 ---NTMTWADLRRRVAALAGALSGRGVGFGDRVMILMLNRTEFVESVLAA
Rv0166|M.tuberculosis_H37Rv ---NTMTWADLRRRVAALAGALSGRGVGFGDRVMILMLNRTEFVESVLAA
Mflv_0027|M.gilvum_PYR-GCK ---RTVSYRELDERSNRTAQAFSRTGVGPGDRVAFVERNGIEFFDVAFGS
MAB_4559|M.abscessus_ATCC_1997 ---REHTVEQFADRVARLSAGLRANGVRPGDRVGMLSLNSDRYIEYLTAV
MMAR_1606|M.marinum_M ---IEIPYGALDAASARLAGLLIQRGLRPGDRVGVMLPNVPYFAVAYYGV
Mvan_0898|M.vanbaalenii_PYR-1 ---DTYSYADVWQRSCDLAGMLHAGGVGAGQTVVSLQDTQIDAIASWFGA
TH_2476|M.thermoresistible__bu ---RRYSYGEANETVNRYANSLRALGVEAGDPVAMLMDNSLEFVFTALGT
MAV_1639|M.avium_104 ---VDVTFAQMRQRSCAAANMLSDLGVGRGDRVALFSGTCPEWVYFWLGA
MSMEG_4036|M.smegmatis_MC2_155 ---EEISRADALRDGSRIAAGLIAKGVRKGDRVVLVAGNGLDFVRAWLGL
MUL_0229|M.ulcerans_Agy99 DAYRQTTYREVGQRAAQLANALRGLGISGDQRVATFMWNNAEHLVTYLAV
MLBr_02661|M.leprae_Br4923 N-RIVVSYRDLLRLVDDLTVQLALGGLLPGDRVALCAASNIEFVVGLLAA
. : : *: .: * . .
Mb0172|M.bovis_AF2122/97 NMIGAIAVPLNFRLTPTEIAVLVEDCAAHVMLTEAALAPVAIGVRNIQPL
Rv0166|M.tuberculosis_H37Rv NMIGAIAVPLNFRLTPTEIAVLVEDCVAHVMLTEAALAPVAIGVRNIQPL
Mflv_0027|M.gilvum_PYR-GCK AKLGAVAVPVNWRLTAPEMRHVIADCGASVVVVGQEFTGRIEAIADDLDV
MAB_4559|M.abscessus_ATCC_1997 PWLGAALNAVNIRWSLAEIGYSLRESGTRVLLVDDTFKAAAAPLRAACAC
MMAR_1606|M.marinum_M LRAGGVVVPMNVLLKERETSFYLTDSEAKAIIAWHEFAPAAQAGADEAGA
Mvan_0898|M.vanbaalenii_PYR-1 NMLGAIWVGTNTALRGEFLRHVITDTGARVVVAEADLVDRIRAIGQHLPD
TH_2476|M.thermoresistible__bu NKLGAMWVPTNTSYRGEWLARTFVDGGAKVLVVDESLLDRATDNGLAR-R
MAV_1639|M.avium_104 ARIGAVSAAINAAHKGDFLLHALRLCRPAVIFTDPEHRSRAERAAAALEG
MSMEG_4036|M.smegmatis_MC2_155 ILCGAVTVSINPKAVASELPAVIDEVRPALVLVEAGLEVGHVEHTPPGGQ
MUL_0229|M.ulcerans_Agy99 PSMGAVLHTLNIRLFAEQIAYVANEAEDQVVLVDLSLTNLLAPVLPNLDT
MLBr_02661|M.leprae_Br4923 SRAGLIVVPLDPALPVNEQCIRSQAAGVRVTLVDSLALEGVSDQRAATMR
* : ..
Mb0172|M.bovis_AF2122/97 LSVIVVAG-GSSQDSVFGYEDLLNEAG------DVHEPVDIPNDSPALIM
Rv0166|M.tuberculosis_H37Rv LSVIVVAG-GSSQDSVFGYEDLLNEAG------DVHEPVDIPNDSPALIM
Mflv_0027|M.gilvum_PYR-GCK EVVVIG-----KHRHWPTFDDLLAAAP------AVDPGVVTGPDDLVFLM
MAB_4559|M.abscessus_ATCC_1997 LETVIYCGAGPAPQDMIGYEDLLASHE------PIDD-TRTGGDSLLGVF
MMAR_1606|M.marinum_M ECILVKPG---------EFEELIQAAE------PLEEAAVREDDDTAVIL
Mvan_0898|M.vanbaalenii_PYR-1 VDLVLQRGDERPAADPHLRIERLVDLG----GHTVNEPGDGAVDDLTCLT
TH_2476|M.thermoresistible__bu FERVVVNGDPAAIADEAVR-QRAVALADFAEGAGTEVTSSADAATISSVM
MAV_1639|M.avium_104 PARIVVQGDSLTATLSRAA-------------DRAPAEDRGDAGELGCLF
MSMEG_4036|M.smegmatis_MC2_155 PVPVMFIDEAHAASDEAVVSLDRPPAG---------------PDDPVSFI
MUL_0229|M.ulcerans_Agy99 VHTVIAVGEGDTAALEESGKTVLRYDELLDGQSTEFDWPHIDENSAAAMC
MLBr_02661|M.leprae_Br4923 YWPIAVSYGSVTGASEGSLLVHLDDTAALH---PVTSTPDGLRHDDAMIM
: .
Mb0172|M.bovis_AF2122/97 YTAGTTGRPKGAVLTHANLTGQAMTALYTSGANINSD-VGFVGVPLFHIA
Rv0166|M.tuberculosis_H37Rv YTSGTTGRPKGAVLTHANLTGQAMTALYTSGANINSD-VGFVGVPLFHIA
Mflv_0027|M.gilvum_PYR-GCK YTSGTTGAPKGVMLSNANVVCKTAGVGGPWKFDADS--VSLAVMPLFHMA
MAB_4559|M.abscessus_ATCC_1997 YTGGTTGNPKGVMLSHNNVLASAMGSLSTGNFLTRGG-RLLHSAPMFHMA
MMAR_1606|M.marinum_M YTSGTTGKPKGAELTHANLRRNVEVIVQMLQVGPDD--VILGALPLFHAF
Mvan_0898|M.vanbaalenii_PYR-1 YTGGTTGPSKGCMISNGYAINIARRGLAQTQRQTDE--LNWSPLPMFHLN
TH_2476|M.thermoresistible__bu WTSGTTGRSKGVMQPYGAWYAAARIFASARDLAPGD--VLYCCVPLFNSG
MAV_1639|M.avium_104 YTSGTTGPSKAVATTWHYLFSVAATVAAAWEFRQGE--VLWTAMPLFHLS
MSMEG_4036|M.smegmatis_MC2_155 QSSGSTGKPKFVIETNRMYTMAGEGFAHWLGLDDED--VLLTTLPLSHLN
MUL_0229|M.ulcerans_Agy99 YTSGTTGHPKGVVYSHRSSFLHSMATCTANGIGLGSSDRVLPIVPMFHAN
MLBr_02661|M.leprae_Br4923 FTGGTTGLPKMVPWTDGNIAGSVHAIITAYQLGPQD--ATVVVMPLYHGH
:.*:** .* . *: :
Mb0172|M.bovis_AF2122/97 GIGNMLTGLLLGLPTVIYPLGA-FDPGQLLDVLEAEKVTGIFLVPAQWQA
Rv0166|M.tuberculosis_H37Rv GIGNMLTGLLLGLPTVIYPLGA-FDPGQLLDVLEAEKVTGIFLVPAQWQA
Mflv_0027|M.gilvum_PYR-GCK GFGWALAGLWEGAVTVVLRD---VDPGAILDAVARHRVTNMLLVPAVIQF
MAB_4559|M.abscessus_ATCC_1997 DFSAVLAGNLSGSTHVIVPS---FSPQGVLDAIVAHDVQDMLLVPTMIQM
MMAR_1606|M.marinum_M GQTAGLNAAVAAGACLTLIPR--FSPEKALQIIERDRVTVFEGVPTMFAA
Mvan_0898|M.vanbaalenii_PYR-1 VLAMTLIGTMLVGGSAALAQR--FSASRFWPEIERTGARVVNLIGGLPAI
TH_2476|M.thermoresistible__bu GWSLNVFQALYSGLSIAIDRE--FSVSRFWDRVRRYGATQLLTLGSMHMY
MAV_1639|M.avium_104 -AAPSVLAPMLVGATTVLAAA--FHPREVWDDIRAHGAVGFAGAGAMVSM
MSMEG_4036|M.smegmatis_MC2_155 AQAYSVLGSWGCGAKLVLLPR--FSASSFWSDVAKYGATVFNAIGAMLEA
MUL_0229|M.ulcerans_Agy99 AWGLPYAALMAG-ADLVLPDRH-LDARSLVHMVENLRPTRAGAVPTIWND
MLBr_02661|M.leprae_Br4923 GLIAALLSTLASGGVVLLPARARFSARTFWDDIDAVAATWYTAAPAIHRI
. :
Mb0172|M.bovis_AF2122/97 VCTEQ--QARPRDLR-LRVLSWGAAPAPDALLRQMSATFPETQILAA-FG
Rv0166|M.tuberculosis_H37Rv VCTEQ--QARPRDLR-LRVLSWGAAPAPDALLRQMSATFPETQILAA-FG
Mflv_0027|M.gilvum_PYR-GCK LLDTT--GLDDTDLSRLRIVVYGASPITDDVLMRGIDRFG-GIFAQV-YG
MAB_4559|M.abscessus_ATCC_1997 LVDHP--GAAQLDLSGIRSITYGASVISEAVLQRATRVFPNARFTQA-YG
MMAR_1606|M.marinum_M MLHST--ERP--DTGSLRLCVSGGAAMPVEVMRGFEQAFG-TMVLEG-YG
Mvan_0898|M.vanbaalenii_PYR-1 LAQQP--DTLEMARCFGQIRMVHAVPFPPALQEIWRNRFGVKVPGSKGYG
TH_2476|M.thermoresistible__bu LWQRE--ERDDDADNP--ARVAGLLPLPAEIREAFQKRFGLER-IWRGYG
MAV_1639|M.avium_104 LQNLP--ADPGDARLP--LRFISAAPIDARSYRDIEKRYGCRI--VTMYG
MSMEG_4036|M.smegmatis_MC2_155 LMAQP--PSVSQERARVRLCYSAPAPAPAR-HREIENRFGFRLVVGYALS
MUL_0229|M.ulcerans_Agy99 VMHYLE-KNPGHDVSSLGLVACGGSAVPESLMRTFEEKYG--VQIRQLWG
MLBr_02661|M.leprae_Br4923 LLELASTQSFRSKRAKLRFIRSCSAPLTQETAQALREEFLAPVICAFGMT
: :
Mb0172|M.bovis_AF2122/97 QTEMSPVTCMLLGEDA------IAKRGSVGRVIPTVAARVVDQNMNDVPV
Rv0166|M.tuberculosis_H37Rv QTEMSPVTCMLLGEDA------IAKRGSVGRVIPTVAARVVDQNMNDVPV
Mflv_0027|M.gilvum_PYR-GCK MTESTGSVTQLDGDEH-----LPQLLRSCGRPYPWVQIRVVDPTGRDVAP
MAB_4559|M.abscessus_ATCC_1997 MTEVSPVATLLLPDDHD----DPALLRSAGRAAPHCEVRIVDPDDNEVPR
MMAR_1606|M.marinum_M LSETSPTASFNHPDRE-------RKPGSIGTPIAGVQMKLLEVDTH----
Mvan_0898|M.vanbaalenii_PYR-1 MTEVFPITYQTPDEQS-------PAESAGRINHEDLEVRIVDERDDDVKP
TH_2476|M.thermoresistible__bu QSEIMPLSVSTPEREG-------NPAGDG-FPRPDVEVALLDERGREVPV
MAV_1639|M.avium_104 MTEAFPIAVKALADAG-------IPGTSG-RPNPDFEVRILDAHGNSLPP
MSMEG_4036|M.smegmatis_MC2_155 ETPYGLIVPVDEPTVYG----SMGVPRQHPTLGAVNEVRVVDADGHEVAD
MUL_0229|M.ulcerans_Agy99 MTETSPLATMAWPPPGTPPDRHWAIRSTQGQPVCGVETRIVDDNGEALPN
MLBr_02661|M.leprae_Br4923 EATHQVTTTNIKWFGQG---ENPTVTNGLVGQSTGVQIRIVGSDGQPLPP
: ::
Mb0172|M.bovis_AF2122/97 G--EVGEIVYR---APTLMSCYWNNPEATAEAFA-GGWFHSGDLVRMDSD
Rv0166|M.tuberculosis_H37Rv G--EVGEIVYR---APTLMSCYWNNPEATAEAFA-GGWFHSGDLVRMDSD
Mflv_0027|M.gilvum_PYR-GCK G--TVGEVWTR---SDQNMLGYWNNPEATAATLTPDGWLRTGDAGYLDRD
MAB_4559|M.abscessus_ATCC_1997 G--EIGEVIVK---GDNVMLGYWELPEESAAAIR-DGWMHTGDAGRMDDR
MMAR_1606|M.marinum_M ---GIGEIAIR---GHNVMKGYWNRPDATAAAIDAEGWFRTGDLARIDED
Mvan_0898|M.vanbaalenii_PYR-1 G--EVGEVICRPRRSHVMFQGYWRRPDAMVDSCR-NLWFHTGDLGRIDDA
TH_2476|M.thermoresistible__bu G--EVGEICVRPRRPDVMFRGYYNNEEGTAKAWR-DGWYHSNDLGRFDEA
MAV_1639|M.avium_104 D--TVGEIACRPRHPHVMSEGYIG--DDLAVRPH-PEWFRTGDLGRLDRD
MSMEG_4036|M.smegmatis_MC2_155 G--TTGELELR---NPAITPGYFGKTTESAAMRP-GGWLRTGDLAVRRPD
MUL_0229|M.ulcerans_Agy99 DGQAVGEVEVR---GPWITGAYYLGYDQSEFDSG---WLRTGDVGRIDEL
MLBr_02661|M.leprae_Br4923 D--TVGEVWLR---GSTVVRGYLGDPAITAANFT-HGWLRTGDLGSLSVT
**: : * * ::.*
Mb0172|M.bovis_AF2122/97 GYVWVVDRKKDMIISGGENIYCAELENVLASHPDIAEVAVIGRADEKWG-
Rv0166|M.tuberculosis_H37Rv GYVWVVDRKKDMIISGGENIYCAELENVLASHPDIAEVAVIGRADEKWG-
Mflv_0027|M.gilvum_PYR-GCK GYLYLHDRIKDMIVSGAENVYPAEVENVLMTHPAVTDVAVIGIPDTRWG-
MAB_4559|M.abscessus_ATCC_1997 GYVYIVDRIKDMVVTGGENVYSAEVENALAKHPAVAACAVIGIPDEQWG-
MMAR_1606|M.marinum_M GYYFIVDRSKDMIIRGGYNVYPREIEEVLYEHPEVREAAVVAVPDDMLG-
Mvan_0898|M.vanbaalenii_PYR-1 GYFYFADRKHDYLRRRGENISSQELETTYVAHPDIVAVAVHAVPS-ELT-
TH_2476|M.thermoresistible__bu GELYFVDRASDYMRHKGRNIPSLELEELIGRHPAVYEVAAHGVPSQDLAE
MAV_1639|M.avium_104 QNLTYVDRIKDALRRRGENISSVEVETVVMGHPAVAEAAAVGVPG-ELG-
MSMEG_4036|M.smegmatis_MC2_155 GHFFFGGRAKEVIRYKGENLSPAEVENAIGSHPAVRAVAVIGVPSALSE-
MUL_0229|M.ulcerans_Agy99 GFITLTDRAKDVIKSGGEWISSVELENCLIAHPDVVEAAVVGVPDERWQ-
MLBr_02661|M.leprae_Br4923 GDLRIRGRIKELINRSGEKISPERVEGVLASHHNVMEVAVFGDPDKVYG-
.* : : . : .:* * : *. . ..
Mb0172|M.bovis_AF2122/97 -EVPIAVAAVTND-DLRIEDLGEFLTDRLARYKHPKALEIVDALPRNPAG
Rv0166|M.tuberculosis_H37Rv -EVPIAVAAVTND-DLRIEDLGEFLTDRLARYKHPKALEIVDALPRNPAG
Mflv_0027|M.gilvum_PYR-GCK -EAVKAVVVAGAP--VTEAELIAFAREQLAGFKLPKSVDFVDALPRNPSG
MAB_4559|M.abscessus_ATCC_1997 -ERVHAVVVKQAQSECCDGDLQEHCREHIANYKVPRSFEFVDELPLSGAG
MMAR_1606|M.marinum_M -EEVGAAIALIPGATSDAAALSDFVKERVAAYKYPRRIWFVDELPKGPTG
Mvan_0898|M.vanbaalenii_PYR-1 EDDVKVTVVLRPGSVLTPAELFEWSKERVPYFALPRYVEFRTELPTSPLG
TH_2476|M.thermoresistible__bu EDEVKICVILREGHELEPEELIRFINERAPYFMVPRYVEFVTELPHTPTG
MAV_1639|M.avium_104 EDDVLVVVTLRPGATLDCAELLDFCADRMPYFCVPRYVETVPELPKNAIG
MSMEG_4036|M.smegmatis_MC2_155 -EDVKAFVQLRPGETASPTELAQWSATKLPPYKRPRYIELVTEFPLTDTQ
MUL_0229|M.ulcerans_Agy99 -ERPLAVVVRREGATVSAAELRKFLADRVVRWWLPERWAFADQVPRTSVG
MLBr_02661|M.leprae_Br4923 -ETVTAVIVPREVIAPTPSELAVFCRDRLAAFEVPTRFQEASALPHTAKG
: * : : * .*
Mb0172|M.bovis_AF2122/97 KVLKTELRLRYGACVNVERRSASAGFTERRENRQKL
Rv0166|M.tuberculosis_H37Rv KVLKTELRLRYGACVNVERRSASAGFTERRENRQKL
Mflv_0027|M.gilvum_PYR-GCK KLLKRQLREPY-----WTDEDRHIG-----------
MAB_4559|M.abscessus_ATCC_1997 KILKRVLRQKY-----WESRDAGVS-----------
MMAR_1606|M.marinum_M KILKRDITVPQ------ASPAQQ-------------
Mvan_0898|M.vanbaalenii_PYR-1 RVHKYLLREQGCTPETWDREAAGVSWDRR-------
TH_2476|M.thermoresistible__bu RVQKFKLRERGVTPDTWDREAAGLVLER--------
MAV_1639|M.avium_104 RIRKDLLRARGLTTNVWDREKHGYVVRR--------
MSMEG_4036|M.smegmatis_MC2_155 KIAKARLPRDRSTSEVDFAPRRDPRQDDRDT-----
MUL_0229|M.ulcerans_Agy99 KYDKKTIRAGYAESVYQVTDVHN-------------
MLBr_02661|M.leprae_Br4923 SLDRRAVAEQFAHRG---------------------
: :