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MRAPADFGVDRFTVPAVLDRRAEQHPDRVMMSIAGVDVTFAQMRQRSCAAANMLSDLGVGRGDRVALFSG TCPEWVYFWLGAARIGAVSAAINAAHKGDFLLHALRLCRPAVIFTDPEHRSRAERAAAALEGPARIVVQG DSLTATLSRAADRAPAEDRGDAGELGCLFYTSGTTGPSKAVATTWHYLFSVAATVAAAWEFRQGEVLWTA MPLFHLSAAPSVLAPMLVGATTVLAAAFHPREVWDDIRAHGAVGFAGAGAMVSMLQNLPADPGDARLPLR FISAAPIDARSYRDIEKRYGCRIVTMYGMTEAFPIAVKALADAGIPGTSGRPNPDFEVRILDAHGNSLPP DTVGEIACRPRHPHVMSEGYIGDDLAVRPHPEWFRTGDLGRLDRDQNLTYVDRIKDALRRRGENISSVEV ETVVMGHPAVAEAAAVGVPGELGEDDVLVVVTLRPGATLDCAELLDFCADRMPYFCVPRYVETVPELPKN AIGRIRKDLLRARGLTTNVWDREKHGYVVRR
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. avium 104 | MAV_1639 | - | - | 100% (521) | AMP-binding enzyme, putative |
| M. avium 104 | MAV_3523 | - | 7e-52 | 31.76% (510) | acyl-CoA synthase |
| M. avium 104 | MAV_1186 | - | 5e-48 | 30.17% (527) | acyl-CoA synthetase |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb0172 | fadD5 | 3e-43 | 29.49% (529) | acyl-CoA synthetase |
| M. gilvum PYR-GCK | Mflv_0027 | - | 2e-50 | 30.71% (495) | AMP-dependent synthetase and ligase |
| M. tuberculosis H37Rv | Rv0166 | fadD5 | 2e-43 | 29.68% (529) | acyl-CoA synthetase |
| M. leprae Br4923 | MLBr_02546 | fadD2 | 3e-33 | 27.08% (506) | acyl-CoA synthetase |
| M. abscessus ATCC 19977 | MAB_4559 | - | 6e-46 | 29.59% (507) | putative long-chain-fatty-acid CoA ligase |
| M. marinum M | MMAR_4412 | fadD14 | 2e-45 | 29.52% (525) | medium chain fatty-acid-CoA ligase FadD14 |
| M. smegmatis MC2 155 | MSMEG_4036 | - | 4e-52 | 31.84% (512) | crotonobetaine/carnitine-CoA ligase |
| M. thermoresistible (build 8) | TH_2476 | - | 1e-70 | 35.17% (489) | PUTATIVE putative AMP-dependent synthetase and ligase |
| M. ulcerans Agy99 | MUL_0229 | fadD14 | 2e-45 | 29.14% (525) | acyl-CoA synthetase |
| M. vanbaalenii PYR-1 | Mvan_0898 | - | 8e-82 | 36.08% (521) | AMP-dependent synthetase and ligase |
CLUSTAL 2.0.9 multiple sequence alignment
Mflv_0027|M.gilvum_PYR-GCK -------------------------------------------MFHNLAD
MAB_4559|M.abscessus_ATCC_1997 ---------------------------------------------MQFTQ
Mb0172|M.bovis_AF2122/97 --------------------MTAQLASHLTRALTLAQQQPYLARRQNWVN
Rv0166|M.tuberculosis_H37Rv --------------------MTAQLASHLTRALTLAQQQPYLARRQNWVN
MMAR_4412|M.marinum_M ---------------------------------MYSTMQDFPLTITAIMR
MUL_0229|M.ulcerans_Agy99 ---------------------------------MYSTMQDFPLTITAIMR
TH_2476|M.thermoresistible__bu ----------------------------------MHWIEDSSRENVAAGR
Mvan_0898|M.vanbaalenii_PYR-1 ------------------------------------MKDNTIKTETTVVG
MAV_1639|M.avium_104 ----------------------------------MRAPADFGVDRFTVPA
MSMEG_4036|M.smegmatis_MC2_155 ------------------------------------------------MS
MLBr_02546|M.leprae_Br4923 MTKLRHYLERGAAEVHYLRSIFESGALGFEPPLTYAAMAVAMRKWGDLAM
Mflv_0027|M.gilvum_PYR-GCK IVRSHGRNRPDAPALIVGDRTVSYRELDERSNRTAQAFSRTGVGPGDRVA
MAB_4559|M.abscessus_ATCC_1997 WLHRALQQYPDRLITIDQGREHTVEQFADRVARLSAGLRANGVRPGDRVG
Mb0172|M.bovis_AF2122/97 QLERHAMMQPDAPALRFVGNTMTWADLRRRVAALAGALSGRGVGFGDRVM
Rv0166|M.tuberculosis_H37Rv QLERHAMMQPDAPALRFVGNTMTWADLRRRVAALAGALSGRGVGFGDRVM
MMAR_4412|M.marinum_M HGCGVHGSRQVITATGDAYRQTTYREVGQRAAQLANALRGLGISGDQRVA
MUL_0229|M.ulcerans_Agy99 HGCGVHGSRQVITATGDAYRQTTYREVGQRAAQLANALRGLGISGDQRVA
TH_2476|M.thermoresistible__bu ALALQADAIGDAPFLASGDRRYSYGEANETVNRYANSLRALGVEAGDPVA
Mvan_0898|M.vanbaalenii_PYR-1 LLRKCVDEHPDTLFLDLAGDTYSYADVWQRSCDLAGMLHAGGVGAGQTVV
MAV_1639|M.avium_104 VLDRRAEQHPDRVMMSIAGVDVTFAQMRQRSCAAANMLSDLGVGRGDRVA
MSMEG_4036|M.smegmatis_MC2_155 YLSRAAARWPDRTWCITPREEISRADALRDGSRIAAGLIAKGVRKGDRVV
MLBr_02546|M.leprae_Br4923 LPAMPARRSPHRAALIDEEGTLTYKELDRAAHALANGLIAKGVRGGDGIA
: : : : *: .: :
Mflv_0027|M.gilvum_PYR-GCK FVERNGIEFFDVAFGSAKLGAVAVPVNWRLTAPEMRHVIADCGASVVVVG
MAB_4559|M.abscessus_ATCC_1997 MLSLNSDRYIEYLTAVPWLGAALNAVNIRWSLAEIGYSLRESGTRVLLVD
Mb0172|M.bovis_AF2122/97 ILMLNRTEFVESVLAANMIGAIAVPLNFRLTPTEIAVLVEDCAAHVMLTE
Rv0166|M.tuberculosis_H37Rv ILMLNRTEFVESVLAANMIGAIAVPLNFRLTPTEIAVLVEDCVAHVMLTE
MMAR_4412|M.marinum_M TFMWNNAEHLVAYLAVPSMGAVLHTLNIRLFAEQIAYVANEAEDQVVLVD
MUL_0229|M.ulcerans_Agy99 TFMWNNAEHLVTYLAVPSMGAVLHTLNIRLFAEQIAYVANEAEDQVVLVD
TH_2476|M.thermoresistible__bu MLMDNSLEFVFTALGTNKLGAMWVPTNTSYRGEWLARTFVDGGAKVLVVD
Mvan_0898|M.vanbaalenii_PYR-1 SLQDTQIDAIASWFGANMLGAIWVGTNTALRGEFLRHVITDTGARVVVAE
MAV_1639|M.avium_104 LFSGTCPEWVYFWLGAARIGAVSAAINAAHKGDFLLHALRLCRPAVIFTD
MSMEG_4036|M.smegmatis_MC2_155 LVAGNGLDFVRAWLGLILCGAVTVSINPKAVASELPAVIDEVRPALVLVE
MLBr_02546|M.leprae_Br4923 ILARNHRWFVIANYGCARVGARIILLNSEFSGPQIKEVSEREGAKVIIYD
. . . . ** * : ::.
Mflv_0027|M.gilvum_PYR-GCK QEFTGRIEAIADDLDVEVVVIG-----------KHRHWPTFDDLLAAAPA
MAB_4559|M.abscessus_ATCC_1997 DTFKAAAAPLRAACACLETVIYCGA------GPAPQDMIGYEDLLASHEP
Mb0172|M.bovis_AF2122/97 AALAPVAIGVRNIQPLLSVIVVAG-------GSSQDSVFGYEDLLNEAGD
Rv0166|M.tuberculosis_H37Rv AALAPVAIGVRNIQPLLSVIVVAG-------GSSQDSVFGYEDLLNEAGD
MMAR_4412|M.marinum_M LSLTNLLAPVLPDLGTVHTVIAVGEGDTAPLEESGKTVLRYDELLDGQPT
MUL_0229|M.ulcerans_Agy99 LSLTNLLAPVLPNLDTVHTVIAVGEGDTAALEESGKTVLRYDELLDGQST
TH_2476|M.thermoresistible__bu ESLLDRATDNGLAR-RFERVVVNGDPAAIADEAVR-QRAVALADFAEGAG
Mvan_0898|M.vanbaalenii_PYR-1 ADLVDRIRAIGQHLPDVDLVLQRGDERPAADPHLRIERLVDLG----GHT
MAV_1639|M.avium_104 PEHRSRAERAAAALEGPARIVVQGDSLTATLSRAA-------------DR
MSMEG_4036|M.smegmatis_MC2_155 AGLEVGHVEHTPPGGQPVPVMFIDEAHAAS---------------DEAVV
MLBr_02546|M.leprae_Br4923 DEYTKVVSKAEPALGKLRALGVNPD------EEEPPGSTDETLAELIARS
:
Mflv_0027|M.gilvum_PYR-GCK VDPGVVTGPDDLVFLMYTSGTTGAPKGVMLSNANVVCKTAGVGGPWKFDA
MAB_4559|M.abscessus_ATCC_1997 IDD-TRTGGDSLLGVFYTGGTTGNPKGVMLSHNNVLASAMGSLSTGNFLT
Mb0172|M.bovis_AF2122/97 VHEPVDIPNDSPALIMYTAGTTGRPKGAVLTHANLTGQAMTALYTSGANI
Rv0166|M.tuberculosis_H37Rv VHEPVDIPNDSPALIMYTSGTTGRPKGAVLTHANLTGQAMTALYTSGANI
MMAR_4412|M.marinum_M EFDWPHIDENSAAAMCYTSGTTGHPKGVVYSHRSSFLHSMATCTANGIGV
MUL_0229|M.ulcerans_Agy99 EFDWPHIDENSAAAMCYTSGTTGHPKGVVYSHRSSFLHSMATCTANGIGL
TH_2476|M.thermoresistible__bu TEVTSSADAATISSVMWTSGTTGRSKGVMQPYGAWYAAARIFASARDLAP
Mvan_0898|M.vanbaalenii_PYR-1 VNEPGDGAVDDLTCLTYTGGTTGPSKGCMISNGYAINIARRGLAQTQRQT
MAV_1639|M.avium_104 APAEDRGDAGELGCLFYTSGTTGPSKAVATTWHYLFSVAATVAAAWEFRQ
MSMEG_4036|M.smegmatis_MC2_155 SLDRPPAGPDDPVSFIQSSGSTGKPKFVIETNRMYTMAGEGFAHWLGLDD
MLBr_02546|M.leprae_Br4923 SSAPAPKVRKRASIIILTSGTTGTPKGANRNTPASLAPLGGILSKVPFRA
. :.*:** .*
Mflv_0027|M.gilvum_PYR-GCK DS--VSLAVMPLFHMAGFGWALAGLWEGAVTVVLRD--VDPGAILDAVAR
MAB_4559|M.abscessus_ATCC_1997 RGG-RLLHSAPMFHMADFSAVLAGNLSGSTHVIVPS--FSPQGVLDAIVA
Mb0172|M.bovis_AF2122/97 NSD-VGFVGVPLFHIAGIGNMLTGLLLGLPTVIYPLGAFDPGQLLDVLEA
Rv0166|M.tuberculosis_H37Rv NSD-VGFVGVPLFHIAGIGNMLTGLLLGLPTVIYPLGAFDPGQLLDVLEA
MMAR_4412|M.marinum_M GSNDRVLPIVPMFHANAWGLPYAALMAG-ADLVLPDRHLDARSLVHMVEN
MUL_0229|M.ulcerans_Agy99 GSSDRVLPIVPMFHANAWGLPYAALMAG-ADLVLPDRHLDARSLVHMVEN
TH_2476|M.thermoresistible__bu GD--VLYCCVPLFNSGGWSLNVFQALYSGLSIAIDR-EFSVSRFWDRVRR
Mvan_0898|M.vanbaalenii_PYR-1 DE--LNWSPLPMFHLNVLAMTLIGTMLVGGSAALAQ-RFSASRFWPEIER
MAV_1639|M.avium_104 GE--VLWTAMPLFHLS-AAPSVLAPMLVGATTVLAA-AFHPREVWDDIRA
MSMEG_4036|M.smegmatis_MC2_155 ED--VLLTTLPLSHLNAQAYSVLGSWGCGAKLVLLP-RFSASSFWSDVAK
MLBr_02546|M.leprae_Br4923 HE--VTLLPAPMFHALGYLHATLAMFLGSTLVLRRR--FKPATVLEDIEK
*: : . . :
Mflv_0027|M.gilvum_PYR-GCK HRVTNMLLVPAVIQFLLDTTGLDD-TDLSRLRIVVYGASPITDDVLMRGI
MAB_4559|M.abscessus_ATCC_1997 HDVQDMLLVPTMIQMLVDHPGAAQ-LDLSGIRSITYGASVISEAVLQRAT
Mb0172|M.bovis_AF2122/97 EKVTGIFLVPAQWQAVCTEQQARP-RDLR-LRVLSWGAAPAPDALLRQMS
Rv0166|M.tuberculosis_H37Rv EKVTGIFLVPAQWQAVCTEQQARP-RDLR-LRVLSWGAAPAPDALLRQMS
MMAR_4412|M.marinum_M LRPTRAGAVPTIWNDVMHYLEKNPGHDVSSLGLVACGGSAVPESLMRTFE
MUL_0229|M.ulcerans_Agy99 LRPTRAGAVPTIWNDVMHYLEKNPGHDVSSLGLVACGGSAVPESLMRTFE
TH_2476|M.thermoresistible__bu YGATQLLTLGSMHMYLWQREERDDDADNP--ARVAGLLPLPAEIREAFQK
Mvan_0898|M.vanbaalenii_PYR-1 TGARVVNLIGGLPAILAQQPDTLEMARCFGQIRMVHAVPFPPALQEIWRN
MAV_1639|M.avium_104 HGAVGFAGAGAMVSMLQNLPADPGDARLP--LRFISAAPIDARSYRDIEK
MSMEG_4036|M.smegmatis_MC2_155 YGATVFNAIGAMLEALMAQPPSVSQERAR--VRLCYSAPAPAPARHREIE
MLBr_02546|M.leprae_Br4923 HQATAMVVVPVMLSRILDTLENLETKSDLSSLRVVFVSGSQLGAELATRA
: .
Mflv_0027|M.gilvum_PYR-GCK DRFG-GIFAQVYGMTESTGSVTQLDGDEH-----LPQLLRSCGRPYPWVQ
MAB_4559|M.abscessus_ATCC_1997 RVFPNARFTQAYGMTEVSPVATLLLPDDHD----DPALLRSAGRAAPHCE
Mb0172|M.bovis_AF2122/97 ATFPETQILAAFGQTEMSPVTCMLLGEDA------IAKRGSVGRVIPTVA
Rv0166|M.tuberculosis_H37Rv ATFPETQILAAFGQTEMSPVTCMLLGEDA------IAKRGSVGRVIPTVA
MMAR_4412|M.marinum_M EKYG-VQIRQLWGMTETSPLATMAWPPPGTPPDRHWAIRSTQGQPVCGVE
MUL_0229|M.ulcerans_Agy99 EKYG-VQIRQLWGMTETSPLATMAWPPPGTPPDRHWAIRSTQGQPVCGVE
TH_2476|M.thermoresistible__bu RFGLER-IWRGYGQSEIMPLSVSTPEREG-------NPAGDG-FPRPDVE
Mvan_0898|M.vanbaalenii_PYR-1 RFGVKVPGSKGYGMTEVFPITYQTPDEQS-------PAESAGRINHEDLE
MAV_1639|M.avium_104 RYGCRI--VTMYGMTEAFPIAVKALADAG-------IPGTSG-RPNPDFE
MSMEG_4036|M.smegmatis_MC2_155 NRFG-FRLVVGYALSETPYGLIVPVDEPTVYG--SMGVPRQHPTLGAVNE
MLBr_02546|M.leprae_Br4923 LEELGPVIYNMYGSTEIAFATIAGPKDLQ-------INSATVGPVVKGVK
:. :*
Mflv_0027|M.gilvum_PYR-GCK IRVVDPTGRDVAPG--TVGEVWTRSDQ---NMLGYWNNPEATAATLTPDG
MAB_4559|M.abscessus_ATCC_1997 VRIVDPDDNEVPRG--EIGEVIVKGDN---VMLGYWELPEESAAAIR-DG
Mb0172|M.bovis_AF2122/97 ARVVDQNMNDVPVG--EVGEIVYRAPT---LMSCYWNNPEATAEAFA-GG
Rv0166|M.tuberculosis_H37Rv ARVVDQNMNDVPVG--EVGEIVYRAPT---LMSCYWNNPEATAEAFA-GG
MMAR_4412|M.marinum_M TRIVDDNGEALPNDGQAVGEVEVRGPW---ITGAYYLGYDQSKFDSG---
MUL_0229|M.ulcerans_Agy99 TRIVDDNGEALPNDGQAVGEVEVRGPW---ITGAYYLGYDQSEFDSG---
TH_2476|M.thermoresistible__bu VALLDERGREVPVG--EVGEICVRPRRPDVMFRGYYNNEEGTAKAWR-DG
Mvan_0898|M.vanbaalenii_PYR-1 VRIVDERDDDVKPG--EVGEVICRPRRSHVMFQGYWRRPDAMVDSCR-NL
MAV_1639|M.avium_104 VRILDAHGNSLPPD--TVGEIACRPRHPHVMSEGYIG--DDLAVRPH-PE
MSMEG_4036|M.smegmatis_MC2_155 VRVVDADGHEVADG--TTGELELRNPA---ITPGYFGKTTESAAMRP-GG
MLBr_02546|M.leprae_Br4923 VKILDDSGKEVPRG--QVGRIFVGNAFP-------FEGYTGGGGKQIIDG
::* : . *.:
Mflv_0027|M.gilvum_PYR-GCK WLRTGDAGYLDRDGYLYLHDRIKDMIVSGAENVYPAEVENVLMTHPAVTD
MAB_4559|M.abscessus_ATCC_1997 WMHTGDAGRMDDRGYVYIVDRIKDMVVTGGENVYSAEVENALAKHPAVAA
Mb0172|M.bovis_AF2122/97 WFHSGDLVRMDSDGYVWVVDRKKDMIISGGENIYCAELENVLASHPDIAE
Rv0166|M.tuberculosis_H37Rv WFHSGDLVRMDSDGYVWVVDRKKDMIISGGENIYCAELENVLASHPDIAE
MMAR_4412|M.marinum_M WLRTGDVGRIDELGFITLTDRAKDVIKSGGEWISSVELENCLIAHPDVVE
MUL_0229|M.ulcerans_Agy99 WLRTGDVGRIDELGFITLTDRAKDVIKSGGEWISSVELENCLIAHPDVVE
TH_2476|M.thermoresistible__bu WYHSNDLGRFDEAGELYFVDRASDYMRHKGRNIPSLELEELIGRHPAVYE
Mvan_0898|M.vanbaalenii_PYR-1 WFHTGDLGRIDDAGYFYFADRKHDYLRRRGENISSQELETTYVAHPDIVA
MAV_1639|M.avium_104 WFRTGDLGRLDRDQNLTYVDRIKDALRRRGENISSVEVETVVMGHPAVAE
MSMEG_4036|M.smegmatis_MC2_155 WLRTGDLAVRRPDGHFFFGGRAKEVIRYKGENLSPAEVENAIGSHPAVRA
MLBr_02546|M.leprae_Br4923 LLSSGDVGYFDEHDLLYISGRDDEMIVSGGENVFPAEVEDLISGHPEVVE
:.* . .* : : .. : *:* ** :
Mflv_0027|M.gilvum_PYR-GCK VAVIGIPDTRWG--EAVKAVVVAGAP--VTEAELIAFAREQLAGFKLPKS
MAB_4559|M.abscessus_ATCC_1997 CAVIGIPDEQWG--ERVHAVVVKQAQSECCDGDLQEHCREHIANYKVPRS
Mb0172|M.bovis_AF2122/97 VAVIGRADEKWG--EVPIAVAAVTND-DLRIEDLGEFLTDRLARYKHPKA
Rv0166|M.tuberculosis_H37Rv VAVIGRADEKWG--EVPIAVAAVTND-DLRIEDLGEFLTDRLARYKHPKA
MMAR_4412|M.marinum_M AAVVGVPDERWQ--ERPLAVVVRREGATVSAAELRKFLADRVVRWWLPER
MUL_0229|M.ulcerans_Agy99 AAVVGVPDERWQ--ERPLAVVVRREGATVSAAELRKFLADRVVRWWLPER
TH_2476|M.thermoresistible__bu VAAHGVPSQDLAEEDEVKICVILREGHELEPEELIRFINERAPYFMVPRY
Mvan_0898|M.vanbaalenii_PYR-1 VAVHAVPS-ELT-EDDVKVTVVLRPGSVLTPAELFEWSKERVPYFALPRY
MAV_1639|M.avium_104 AAAVGVPG-ELG-EDDVLVVVTLRPGATLDCAELLDFCADRMPYFCVPRY
MSMEG_4036|M.smegmatis_MC2_155 VAVIGVPSALSE--EDVKAFVQLRPGETASPTELAQWSATKLPPYKRPRY
MLBr_02546|M.leprae_Br4923 ATAIGVDDKEWG--ARLRAFVVKKQDATIDEDAIKAYVRDHLARYKVPRE
:. . . . : : : *.
Mflv_0027|M.gilvum_PYR-GCK VDFVDALPRNPSGKLLKRQLREPY-----WTDEDRHIG-----------
MAB_4559|M.abscessus_ATCC_1997 FEFVDELPLSGAGKILKRVLRQKY-----WESRDAGVS-----------
Mb0172|M.bovis_AF2122/97 LEIVDALPRNPAGKVLKTELRLRYGACVNVERRSASAGFTERRENRQKL
Rv0166|M.tuberculosis_H37Rv LEIVDALPRNPAGKVLKTELRLRYGACVNVERRSASAGFTERRENRQKL
MMAR_4412|M.marinum_M WAFADQVPRTSVGKYDKKTIRAGYAEGVYQVTEVHN-------------
MUL_0229|M.ulcerans_Agy99 WAFADQVPRTSVGKYDKKTIRAGYAESVYQVTDVHN-------------
TH_2476|M.thermoresistible__bu VEFVTELPHTPTGRVQKFKLRERGVTPDTWDREAAGLVLER--------
Mvan_0898|M.vanbaalenii_PYR-1 VEFRTELPTSPLGRVHKYLLREQGCTPETWDREAAGVSWDRR-------
MAV_1639|M.avium_104 VETVPELPKNAIGRIRKDLLRARGLTTNVWDREKHGYVVRR--------
MSMEG_4036|M.smegmatis_MC2_155 IELVTEFPLTDTQKIAKARLPRDRSTSEVDFAPRRDPRQDDRDT-----
MLBr_02546|M.leprae_Br4923 VIFLEELPRNPTGKVLKRELRDFEVH-----------------------
.* . : * :