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M. smegmatis MC2 155 MSMEG_5574 (-)

annotation: substrate binding protein
coordinates: 5662068 - 5663411
length: 447

KRLRRLAACIAAAGLTATAGCAGAGTLGATDQTVTIAMVSNSQMTDARELSSRFEAENPDIKLRFVTLSE
NQARAKITASTAMGGGEFDVVMISNYETPQWAENGWLVDLSDYARNTPGYDEDDFIPSLKDSLSYQGDMY
SVPFYGESSFLMYRKDLFEQAGIEMPRTPTWQQVADAAAELNGPDMVGICLRGKPGWGEVLAPLDTVINT
FGGRWFDMDWNAQLTSPEVRKAVQFYVDTVREYGEPGASAAGFGECATQFAQGRAAMWYDATSAVSTVED
PASSNVVGKVGYAMAPTMEKPNSGWLYTWALGIPKSSDKPDAAWKFISWMTDKDYIHLVGEELGWARVPP
GSRLSTYEIPEYKEASAAFGQVTLDSIEGADPAKPTVQPVPYTGVQFLAIPEFQDLGTRVSQQISAAIAG
QKSVDEALEQAQKYAEVVGRSYQEKP*
Operon Prediction Model: Genebank

Paralogs
speciesidgenee-valueidentity (len)annotation
M. smegmatis MC2 155MSMEG_5574--100% (447)substrate binding protein
M. smegmatis MC2 155MSMEG_3111-1e-3227.03% (444) extracellular solute-binding protein, family protein 1
M. smegmatis MC2 155MSMEG_0553-2e-2026.20% (439) sugar ABC transporter, substrate-binding protein, putative

Closest Orthologs (e-value cutoff: 1e-4)
speciesidgenee-valueidentity (len)annotation
M. bovis AF2122 / 97Mb2857cugpB3e-1424.93% (361) sn-glycerol-3-phosphate-binding lipoprotein UGPB
M. gilvum PYR-GCKMflv_3775-1e-3627.75% (436) extracellular solute-binding protein
M. tuberculosis H37RvRv2833cugpB3e-1525.21% (361) sn-glycerol-3-phosphate-binding lipoprotein UGPB
M. leprae Br4923MLBr_01427-6e-1825.56% (360) putative ABC-transport lipoprotein
M. abscessus ATCC 19977MAB_1715c-5e-1122.17% (415) sugar ABC transporter, sugar-binding protein
M. marinum MMMAR_3014-4e-1626.49% (370) sugar-binding lipoprotein
M. avium 104MAV_2476-5e-1726.40% (356) sugar ABC transporter, sugar-binding protein
M. thermoresistible (build 8)TH_2011-0.080.41% (444) substrate binding protein
M. ulcerans Agy99-----
M. vanbaalenii PYR-1Mvan_2624-2e-3426.80% (444) extracellular solute-binding protein

CLUSTAL 2.0.9 multiple sequence alignment


MSMEG_5574|M.smegmatis_MC2_155      --------------MKR--LRRLAACIAAAGLTATAGCAGAG--------
TH_2011|M.thermoresistible__bu      --------------VRRTPIRAVLAAVAAVCLTLTG-CAGAG--------
Mflv_3775|M.gilvum_PYR-GCK          MRFAQIPT-ARRGGK--TARSAVLALLAVLALVLSA-CAGSGGPEQAEST
Mvan_2624|M.vanbaalenii_PYR-1       MRITQWPTSGRRSGRPGTAFSAVMALVAVLALVLTG-CAGSGGPEQAEAT
Mb2857c|M.bovis_AF2122/97           ---------------MDPLNRRQFLALAAAAAGVTAGCAGMGG-------
Rv2833c|M.tuberculosis_H37Rv        ---------------MDPLNRRQFLALAAAAAGVTAGCAGMGG-------
MLBr_01427|M.leprae_Br4923          -------------MHGKLFGRR---SLLRGAGALTAAALAPGA-------
MMAR_3014|M.marinum_M               -------------MFDKPLRRR---ELLRGAGLLTAAALAPVA-------
MAV_2476|M.avium_104                -------------MLDRPFGRR---SLLRGAGALSAAALAPWS-------
MAB_1715c|M.abscessus_ATCC_199      ----------------MKASTRAALTLALVALLLFGVAAWLGIP------
                                                              :        . .            

MSMEG_5574|M.smegmatis_MC2_155      ----TLGATDQTVTIAMVSNSQMTDARELSSRFEAENPDIKLRFVTLSEN
TH_2011|M.thermoresistible__bu      ----TLGSSGNTVTIALVSNSQMSDAQRLAPHFEAANPDIRLKFVTLSEN
Mflv_3775|M.gilvum_PYR-GCK          GSGEVSADTSGTVRILMENVPDTDIVKSMVADFNAEYPGVEINIESLTFD
Mvan_2624|M.vanbaalenii_PYR-1       GTGEVSTDVSGTVRILMENVPDTDIVKSMVADFNKEYPGVEINIESLTFD
Mb2857c|M.bovis_AF2122/97           -GGSVKSGSGPIDFWSSHPGQSSAAERELIGRFQDRFPTLSVKLIDAGKD
Rv2833c|M.tuberculosis_H37Rv        -GGSVKSGSGPIDFWSSHPGQSSAAERELIGRFQDRFPTLSVKLIDAGKD
MLBr_01427|M.leprae_Br4923          -VGCSSDDD-ALTFFFAANPEETNARMRIVGEFQRDHPDIKVRAVLSGPG
MMAR_3014|M.marinum_M               -AGCASDDD-ALTFFFAANPEEADARIRVVDAFGRSHPDIKVRTLLSGPG
MAV_2476|M.avium_104                -AGCGSDDDGALTFFFAANPEERDARMRIIDEFARRHPDIKVRAVLSGPG
MAB_1715c|M.abscessus_ATCC_199      ----TTPHGKTVVTVRVWDQQVAEAYRGSFDEFSRRNPDIQVAVTVTSYA
                                                                    *    * : :        

MSMEG_5574|M.smegmatis_MC2_155      -QARAKITASTAMGGGEFDVVMISNYETPQWAENGWLVDLSDYARNTP--
TH_2011|M.thermoresistible__bu      -QARAKITASTAMGGGEFDVVMISNYETPQWAADGWLVNLSDYARQTP--
Mflv_3775|M.gilvum_PYR-GCK          -QMRDKLVSSFQSSSPAYDLIVVDNPWMVDFANAKFLQPLDARIDSTP--
Mvan_2624|M.vanbaalenii_PYR-1       -QMRDKLVSSFQSSSPTYDLIVVDNPWMVDFANAKFLQPLDARIDSTP--
Mb2857c|M.bovis_AF2122/97           YDEVAQKFNAALIGTDVPDVVLLDDRWWFHFALIGVLTALDDLFGQVG--
Rv2833c|M.tuberculosis_H37Rv        YDEVAQKFNAALIGTDVPDVVLLDDRWWFHFALSGVLTALDDLFGQVG--
MLBr_01427|M.leprae_Br4923          ---VMQQLSTFCAGGKCPDVLMAWDLTYAELADRGVLLDLNTLLGQDKAF
MMAR_3014|M.marinum_M               ---ALQQISTFCAGGKCPDVLMAWELSYAELADRGVFLDLNTMLARDQKF
MAV_2476|M.avium_104                ---VMQQLSTFCVGGRCPDVLMAWELSYAELADRGVLLDLGPLLARDKAF
MAB_1715c|M.abscessus_ATCC_199      --SYFNSLRTDVAGHGADDIFWLSNAYLSDYADTGNLVPVEPRADWDP--
                                         :   :   .    *:.   :    . *    :  :          

MSMEG_5574|M.smegmatis_MC2_155      -GYDEDDFIPSLKDSLSYQGDMYSVPFYGES-SFLMYRKDLFEQAGIEMP
TH_2011|M.thermoresistible__bu      -GYDEDDFIPSIRESLSYEGDMYAVPFYGES-SFLMYRKDLFEKSGIDMP
Mflv_3775|M.gilvum_PYR-GCK          -DYDAGDFFTPLTDITTVDGTRYGVPFYNYA-LGYLYNADDLAAANQQVP
Mvan_2624|M.vanbaalenii_PYR-1       -DYDAADFFKPLTDITTVDGARYGVPFYNYA-LGYLYNADDLTAANQQVP
Mb2857c|M.bovis_AF2122/97           --VDTTDYVDSLLADYEFNGRHYAVPYARST-PLFYYNKAAWQQAGLPD-
Rv2833c|M.tuberculosis_H37Rv        --VDTTDYVDSLLADYEFNGRHYAVPYARST-PLFYYNKAAWQQAGLPD-
MLBr_01427|M.leprae_Br4923          AAELKSDSIEPLYETFTFNGGQYAFPEQWSG-NYLFYNKQLFTNAGVQPP
MMAR_3014|M.marinum_M               ADELAADSIGALYDTFAYNGGQYAFPEQWSG-NFLFYNRGLFADGGVPPP
MAV_2476|M.avium_104                AQQLQADSIPALYETFTFNGKQYALPEQWSG-NYLFYNKRLFDEAGVPSP
MAB_1715c|M.abscessus_ATCC_199      ----------SVVAQFTRDGKLWGVPQLSDAGIALYYNKNLLDAAQVDPA
                                              .:      :*  :..*          *.      .     

MSMEG_5574|M.smegmatis_MC2_155      R-----TPTWQQVADAAAELNGPDMVGICLRGKPGWGEVLAPLDTVINTF
TH_2011|M.thermoresistible__bu      H-----NPTWQQVADAAAALDTPDMAGICLRGKPGWGEVLAPLDTVINTF
Mflv_3775|M.gilvum_PYR-GCK          -------TTLDELVSTSKALKSGDRAGIAMQPQRGY-KIFEEWGNWLFAA
Mvan_2624|M.vanbaalenii_PYR-1       -------TTLDELVSTSKALKSGDRAGIAMQPQRGY-KIFEEWGNWLFAA
Mb2857c|M.bovis_AF2122/97           ----RGLQSWSEFDEWGPELQRVVGAGRSAHG-----WANADLISWTFQG
Rv2833c|M.tuberculosis_H37Rv        ----RGPQSWSEFDEWGPELQRVVGAGRSAHG-----WANADLISWTFQG
MLBr_01427|M.leprae_Br4923          PCTWEQPWSFTEFLDTARALTKRDSSGRVTQWGFVNTWLSYYTAGLFALN
MMAR_3014|M.marinum_M               PTSWDQPWSFAEFLDTARALTKRDGAGRVTQWGFVDTFVPPLSAGLFALN
MAV_2476|M.avium_104                PTAWERPWDFSEFLDTARALTKRDASGRAAQYGFVNTWGSYYSAGLFAMN
MAB_1715c|M.abscessus_ATCC_199      ELAELRWDPDPEVDTLRPMLHRLTAPG-----HWGYNAANDLQGIYLNYL
                                               :.      *      *                       

MSMEG_5574|M.smegmatis_MC2_155      G----GRWFDMDWNAQLTSPEVRKAVQFYVDTVREYGEPGASAAG---FG
TH_2011|M.thermoresistible__bu      G----GRWYDMDWNAQLDSPEVEEAVSFYVNLVRDHGEPGPATSG---FG
Mflv_3775|M.gilvum_PYR-GCK          G----GSIYDADGKITLNTPEAKRALEAYIDTYNTAAPANSLSWG---MD
Mvan_2624|M.vanbaalenii_PYR-1       G----GSIYDADGKITLNTPEAKRALEAYIDTYNTAAPANSLSWG---MD
Mb2857c|M.bovis_AF2122/97           PNWAFGGAYSDKWTLTLTEPATIAAGNFYRNSIHGKGYAAVAN-------
Rv2833c|M.tuberculosis_H37Rv        PNWAFGGAYSDKWTLTLTEPATIAAGNFYRNSIHGKGYAAVAN-------
MLBr_01427|M.leprae_Br4923          NGVPWSNPRMNPTHLNFDDDAFIEAVQFYCDLTNKYQVAPDASEQQ--WM
MMAR_3014|M.marinum_M               NGVPWSTPRMNPTHLNFDDDAFIEAVQFYADLSAKHQVAPTASELQ--SI
MAV_2476|M.avium_104                NGVPWSDPRLNPTHFNFDNAAFQEAVQFYADLANKYRVAPNASETQ--SM
MAB_1715c|M.abscessus_ATCC_199      G--SAGAVFQADDKFAFAKPRAEMAFTYLVDLINVDRVAPSAADTNDNND
                                         .          :       *     :       .           

MSMEG_5574|M.smegmatis_MC2_155      ECATQFAQGRAAMWYDATSAVSTVEDPASSNVVGKVGYAMAPTMEKP---
TH_2011|M.thermoresistible__bu      ECATQFAQGRAAMWYDATSAVSVLEDPAQSNVVGKVGYALAPTVVKA---
Mflv_3775|M.gilvum_PYR-GCK          EAQRSVSANQSASMINYNWQLPALNEPGSGPAAGKIKLATIPGGKQ----
Mvan_2624|M.vanbaalenii_PYR-1       EAQRSVSANQAASMINYNWQLPALNEPGSGPAAGKIKLATIPGGKQ----
Mb2857c|M.bovis_AF2122/97           DIANEFATGILASAVASTGSLPGITASAR-FDFGAAPLPTGPDAAP----
Rv2833c|M.tuberculosis_H37Rv        DIANEFATGILASAVASTGSLAGITASAR-FDFGAAPLPTGPDAAP----
MLBr_01427|M.leprae_Br4923          ATADLFSLGKAAIALGGHWRYQTFMRAEG-LDFDVTSLPIGPSAGTVPAT
MMAR_3014|M.marinum_M               ATTDLFSLGKAAMALGGHWRYQTFDRADG-LEFDVTALPLGPNG------
MAV_2476|M.avium_104                STPNLFAVGRAAMALGGHWRYQTYLRAEG-LDFDVAPLPVGPAVGK----
MAB_1715c|M.abscessus_ATCC_199      FSRNQFLQGRMALFQSGTYNLAQIQANAT-FPWDVAMMPAGPQGRVS---
                                         .  .  *                     .    .  *        

MSMEG_5574|M.smegmatis_MC2_155      ---NSGWLYTWALGIPK--SSDKPDAAWKFISWMTDKDYIHLVGEELGWA
TH_2011|M.thermoresistible__bu      ---DAGWLYTWALGIPA--SSDNKDSAWKFISWMTDKNYIRLVGEELGWA
Mflv_3775|M.gilvum_PYR-GCK          ------VLGSWSWAIPA--NSATPDAAWAFVSWITAK-----PNDVVRTE
Mvan_2624|M.vanbaalenii_PYR-1       ------VLGSWSWAIPA--NSATPDAAWAFVSWITAK-----PNDVVRTE
Mb2857c|M.bovis_AF2122/97           ---ACP-TGGAGLAIPAKLSEERKVNALKFIAFVTNPTNTAYFSQQTGYL
Rv2833c|M.tuberculosis_H37Rv        ---ACP-TGGAGLAIPAKLSEERKVNALKFIAFVTNPTNTAYFSQQTGYL
MLBr_01427|M.leprae_Br4923          RSGACSDIGATGLAIAA--SSSRKEQAWEFVKFATGPAGQALIGESCLFV
MMAR_3014|M.marinum_M               -RAACSNIGATGLAIAA--SSARKEQAWEFVKFATGPAGQALIGETDLFV
MAV_2476|M.avium_104                GQPACSDIGATGLAISS--SSPRKEQAWEFVKFATGPVGQALIGESCLFV
MAB_1715c|M.abscessus_ATCC_199      --------VTNGIVAAANSSSPHPDAVHKVLAWMGSTDGNSFLGRSGSAI
                                               .   .   ..     .  .: :          .      

MSMEG_5574|M.smegmatis_MC2_155      RVPPGSRLSTYEIPEYKEASAAFGQVTLDSIEGADPAKPTVQPVPYTGVQ
TH_2011|M.thermoresistible__bu      RVPPGNRLSTYQIPEYQQASEAFGQVTLESLENADTQHPTVQPVPYTGVQ
Mflv_3775|M.gilvum_PYR-GCK          KGGAAIRKSTLQNPAVLQG--QFGEEYYRTVEQLLADAAPLTQGPSG---
Mvan_2624|M.vanbaalenii_PYR-1       KGGAAIRQSTLQDPAVLGG--QFGEEYYRTVEQLLANAAPLTQGPSG---
Mb2857c|M.bovis_AF2122/97           PVRKSAVDDASERHYLADNPRARVALDQLPHTRTQDYARVFLP-------
Rv2833c|M.tuberculosis_H37Rv        PVRKSAVDDASERHYLADNPRARVALDQLPHTRTQDYARVFLP-------
MLBr_01427|M.leprae_Br4923          PVLQSAIYST----GFAKAHNRVANLAVLTGGPVHSAGLPITP-------
MMAR_3014|M.marinum_M               PVLGSALNSA----GFAKAHRRVDNLAVLTAGPGHSQGLPITP-------
MAV_2476|M.avium_104                PVLRSALKSD----GFARAHRRVGNLGVLTDGPAFSQGLPITP-------
MAB_1715c|M.abscessus_ATCC_199      PAVLSARAPYFQYWADKGVDVSPFFEVLRGQQIAAPGGQGFGAG------
                                        .                                   .         

MSMEG_5574|M.smegmatis_MC2_155      FLAIPEFQDLGTRVSQQISAAIAGQKSVDEALEQAQKYAEVVGRSYQEKP
TH_2011|M.thermoresistible__bu      FLAIPEFQDLGTRVSQQISAAIAGQKSVKDALAQAQKYAQVVGKTYQEKP
Mflv_3775|M.gilvum_PYR-GCK          -------EEMIQAVGTALNEAVAGEKSVDDALATAQAEAEKIQG------
Mvan_2624|M.vanbaalenii_PYR-1       -------EEMIQAVGTELNEAVAGKKSVDDALAAAQAEAEKIQG------
Mb2857c|M.bovis_AF2122/97           -----GGDRIISAGLESIG--LRGADVTKTFTNIQKRLQVILDRQIMRKL
Rv2833c|M.tuberculosis_H37Rv        -----GGDRIISAGLESIG--LRGADVTKTFTNIQKRLQVILDRQIMRKL
MLBr_01427|M.leprae_Br4923          -----AWEKINALMDRNFGPVLRGVRPATSLAGLARAVDEVLNSP-----
MMAR_3014|M.marinum_M               -----AWPKVHALMDRNFGPVLRGSRPATSLIGMSRALDEVLRSP-----
MAV_2476|M.avium_104                -----AWEKVNALMDRNFGPILRGSRPATSLAGLSRSVDEVLRSP-----
MAB_1715c|M.abscessus_ATCC_199      ----------FAALKPYFAEMFLGRLDVREALQQAQRAANRALER-----
                                                     :   . *   .       :              

MSMEG_5574|M.smegmatis_MC2_155      ----
TH_2011|M.thermoresistible__bu      ----
Mflv_3775|M.gilvum_PYR-GCK          ----
Mvan_2624|M.vanbaalenii_PYR-1       ----
Mb2857c|M.bovis_AF2122/97           AGHG
Rv2833c|M.tuberculosis_H37Rv        AGHG
MLBr_01427|M.leprae_Br4923          ----
MMAR_3014|M.marinum_M               ----
MAV_2476|M.avium_104                ----
MAB_1715c|M.abscessus_ATCC_199      ----