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MHPLSRRIAEVMALDASAPAIQFERRWVTWGQIAESARHVSTVVGSGTAGIMLRNTPSHVAALLGVLSGG GTVVVINPSRGDERTRADIEALQLPTLIGLAEDLESLAPGTTATTMALGDIDTTPEITGGSPSEGQHRPG VAVRMPTSGTTGPPKRVDLTYDMLARSVMGRDPDTAPSPTELRQGVAIVNSPLVHVGGVFRILLCLAEAR PFVLLPKFELHSWAEAVREHRPAAVSLVPAALRMVLHSDLTRDDLAGVRVVTSGTAPLSADDAEAFTAKF GIPVLTSYAATEFGGGVAGWTLADHRKYWQDKRGSVGRANPGARLRVVDDNRDPVPPGEPGLLEVIPAQL ADTGEWMQTTDVARIDEDGFLWILGRADQAIIRGGFKVMPDDVRSALESHAAVAGAAVVGRPDPRLGETP VALVELRPGATASPEELSAYLQTRLARYEIPTALILTDAIPRTPSGKPDLTAVRARLAQP
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. gilvum PYR-GCK | Mflv_4362 | - | - | 100% (480) | AMP-dependent synthetase and ligase |
| M. gilvum PYR-GCK | Mflv_1694 | - | 1e-76 | 36.29% (496) | AMP-dependent synthetase and ligase |
| M. gilvum PYR-GCK | Mflv_0714 | - | 3e-36 | 26.35% (501) | acyl-CoA synthetase |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb0172 | fadD5 | 3e-36 | 27.56% (508) | acyl-CoA synthetase |
| M. tuberculosis H37Rv | Rv0166 | fadD5 | 2e-36 | 31.28% (374) | acyl-CoA synthetase |
| M. leprae Br4923 | MLBr_02661 | - | 8e-30 | 30.19% (361) | acyl-CoA synthetase |
| M. abscessus ATCC 19977 | MAB_4569c | - | 2e-40 | 28.32% (512) | fatty-acid-CoA ligase FadD |
| M. marinum M | MMAR_4025 | - | 1e-170 | 64.36% (477) | long-chain fatty-acid CoA ligase |
| M. avium 104 | MAV_1580 | - | 1e-173 | 64.85% (478) | AMP-dependent synthetase and ligase |
| M. smegmatis MC2 155 | MSMEG_2217 | - | 0.0 | 73.70% (479) | AMP-dependent synthetase and ligase |
| M. thermoresistible (build 8) | TH_3559 | - | 1e-141 | 73.21% (336) | PUTATIVE AMP-dependent synthetase and ligase |
| M. ulcerans Agy99 | MUL_3890 | - | 1e-167 | 64.15% (477) | long-chain fatty-acid CoA ligase |
| M. vanbaalenii PYR-1 | Mvan_1982 | - | 0.0 | 76.20% (479) | AMP-dependent synthetase and ligase |
CLUSTAL 2.0.9 multiple sequence alignment
Mb0172|M.bovis_AF2122/97 -MTAQLASHLTRALTLAQQQPYLARRQNWVNQLERHAMMQPDAPALRFVG
Rv0166|M.tuberculosis_H37Rv -MTAQLASHLTRALTLAQQQPYLARRQNWVNQLERHAMMQPDAPALRFVG
MAB_4569c|M.abscessus_ATCC_199 -MTDTIDATVQSAG---LAQPYRARRQNWCNQLARHALMQPDATAIRYLG
Mflv_4362|M.gilvum_PYR-GCK -------------------------MHPLSRRIAEVMALDASAPAIQFER
Mvan_1982|M.vanbaalenii_PYR-1 -----------------------MMVHRLSQRIDDVLALAPAGRAIQFDG
MSMEG_2217|M.smegmatis_MC2_155 ------------------------MAHPLSQRIADVLDLDPDAGAIQFDG
MAV_1580|M.avium_104 -----------------------MPRHPLVQRIADVLDLEPHGRAIEYGG
TH_3559|M.thermoresistible__bu --------------------------------------------------
MMAR_4025|M.marinum_M -----------------------MSNHPLSQRIADTLDLQPDARAVEYDR
MUL_3890|M.ulcerans_Agy99 -----------------------MSNHPLSQRIAVTLDLQPDARAVEYDR
MLBr_02661|M.leprae_Br4923 MKSESITSSRAPVEQIDARLTGQFESPSIASLVEAAAIRNPSAPALVVTD
Mb0172|M.bovis_AF2122/97 --NTMTWADLRRRVAALAGALSGRGVGFGDRVMILMLNRTEFVESVLAAN
Rv0166|M.tuberculosis_H37Rv --NTMTWADLRRRVAALAGALSGRGVGFGDRVMILMLNRTEFVESVLAAN
MAB_4569c|M.abscessus_ATCC_199 --HSISWAELHQRVQALADALSRRGVGFGDRVLILMLNRPEYVESWLAIN
Mflv_4362|M.gilvum_PYR-GCK --RWVTWGQIAESARHVSTVVGS------GTAGIMLRNTPSHVAALLGVL
Mvan_1982|M.vanbaalenii_PYR-1 --DWVTWEQIGTLAQRISDVVGT------ARAGIMLRNRPVHVAALLGVL
MSMEG_2217|M.smegmatis_MC2_155 --QWSCWGQIAALAGHIEKITGP------ARVGIMLRNHPVHVAALLGVL
MAV_1580|M.avium_104 --QWFSWAQLGATARQVAARTTG------TEVGMLLRNRPWQVAAFLGVL
TH_3559|M.thermoresistible__bu --------------------------------------------------
MMAR_4025|M.marinum_M --AWLSWGQLGAATRRVAALAARQPS---PEIGILLRSRPAQMAAFLGVL
MUL_3890|M.ulcerans_Agy99 --AWLSLGQLGAATRRVAALAAKQPS---PEIGILLRNRPAQMAAFLGVL
MLBr_02661|M.leprae_Br4923 NRIVVSYRDLLRLVDDLTVQLALGGLLPGDRVALCAASNIEFVVGLLAAS
Mb0172|M.bovis_AF2122/97 MIGAIAVPLNFRLTPTEIAVLVEDCAAHVMLTE-AALAPVAIGVRNIQPL
Rv0166|M.tuberculosis_H37Rv MIGAIAVPLNFRLTPTEIAVLVEDCVAHVMLTE-AALAPVAIGVRNIQPL
MAB_4569c|M.abscessus_ATCC_199 ELGAIAIPVNFRMTPPEVAFLVENSGAKVAITE-TVLAPVAAAVRQQAPA
Mflv_4362|M.gilvum_PYR-GCK SGGGTVVVINPSRG---------DERTRADIEA-LQLPTLIGLAEDLESL
Mvan_1982|M.vanbaalenii_PYR-1 RGGGTVVVINPSRG---------DDRTRSDVEA-LNLPIIIGSSEDLAAL
MSMEG_2217|M.smegmatis_MC2_155 AAGGTVVVINPARG---------DDRTRADLEA-LALPVVVGLAEDLAHL
MAV_1580|M.avium_104 LGGGTVVVVNPSRG---------DERTRADLAR-LRLPLIIGEPDDLAAL
TH_3559|M.thermoresistible__bu --------------------------------------------------
MMAR_4025|M.marinum_M LSGATVVTINPSRG---------DERTRADIAA-LELPLIAGSDEDLGLL
MUL_3890|M.ulcerans_Agy99 LAGATVVTINPSRG---------DERTRADIAA-LELPLIAGSDEDLGLL
MLBr_02661|M.leprae_Br4923 RAGLIVVPLDPALPVNEQCIRSQAAGVRVTLVDSLALEGVSDQRAATMRY
Mb0172|M.bovis_AF2122/97 LSVIVVAGGSSQDSVFGYEDLLNEAGDVHEPVDIPNDS---PALIMYTAG
Rv0166|M.tuberculosis_H37Rv LSVIVVAGGSSQDSVFGYEDLLNEAGDVHEPVDIPNDS---PALIMYTSG
MAB_4569c|M.abscessus_ATCC_199 LETVIVAGSASENGVLGYEEVIAEPGEPHAEVDLPGDT---PALIMYTSG
Mflv_4362|M.gilvum_PYR-GCK APGTT--ATTMALGDIDTTPEITGGS-PSEGQHRPG-----VAVRMPTSG
Mvan_1982|M.vanbaalenii_PYR-1 APDTA--ATTVAVDGFDEPPTLHRGN-ASDDAGRPG-----VAVWMLTSG
MSMEG_2217|M.smegmatis_MC2_155 MPAHSPRTTRVAIAGLDTEPVVTAAESPATDAGGPG-----VAVWMLTSG
MAV_1580|M.avium_104 VT--DDTPTMPISRLADPP---GPAA--PPAAAPRS-----VAVRMLTSG
TH_3559|M.thermoresistible__bu -------------------------------------------VRMLTSG
MMAR_4025|M.marinum_M VTNTTDSATVSLSDLFDHP---GEPGDHTTRGAGAD-----VAVRMLTSG
MUL_3890|M.ulcerans_Agy99 VTNTTDSATVSLSDLFDHP---GEPGGHTTRGAGAD-----VAVRMLTSG
MLBr_02661|M.leprae_Br4923 WPIAVSYGSVTGASEGSLLVHLDDTAALHPVTSTPDGLRHDDAMIMFTGG
: * *.*
Mb0172|M.bovis_AF2122/97 TTGRPKGAVLTHANLTGQAMTALYTSG---ANINSDVGFVGVPLFHIAGI
Rv0166|M.tuberculosis_H37Rv TTGRPKGAVLTHANLTGQAMTALYTSG---ANINSDVGFVGVPLFHIAGI
MAB_4569c|M.abscessus_ATCC_199 TTGRPKGAVLTHLNLSGQAMTYLLSTT---VDLNSDVGFIGVPMFHIAGV
Mflv_4362|M.gilvum_PYR-GCK TTGPPKRVDLTYDMLARSVMGRDPDTAPSPTELRQGVAIVNSPLVHVGGV
Mvan_1982|M.vanbaalenii_PYR-1 TTGPPKRVDLTYDMLARSVMGPDSDHDSAPTDLRRGVAIVNSPLVHVGGV
MSMEG_2217|M.smegmatis_MC2_155 TTGPPKRVDLTYDMLARSVMGREPEKCPAPTELRRGVAIVNSPLVHVGGV
MAV_1580|M.avium_104 TTGPPKRIDLGYDMLARSVLGVEPGTAPAPTEPRRGVAIVNSPLVHIGGV
TH_3559|M.thermoresistible__bu TTGPPKRVDLTYDMLAHSVIGPDFREAGPPAELRRGVAIVNAPLVHIGGV
MMAR_4025|M.marinum_M TTGPPKRVDLSYDMLARSVMGPAPDRDPAPTEPRRGVAIVNSPLVHIGGV
MUL_3890|M.ulcerans_Agy99 TTGPPNRVDLSYDMLARSVMGPAPDRDPAPTEPRRGVAIVNSPLVHIGGV
MLBr_02661|M.leprae_Br4923 TTGLPKMVPWTDGNIAGSVHAIITAYQLGPQDAT----VVVMPLYHGHGL
*** *: :: .. : .: *: * *:
Mb0172|M.bovis_AF2122/97 GNMLTGLLLGLPTVIYPLGA-FDPGQLLDVLEAEKVTGIFLVPAQWQAVC
Rv0166|M.tuberculosis_H37Rv GNMLTGLLLGLPTVIYPLGA-FDPGQLLDVLEAEKVTGIFLVPAQWQAVC
MAB_4569c|M.abscessus_ATCC_199 GNMITGLLLGLPTVIHPLGA-FDPGALLDVLESEGVTGIFLVPAQWQAVC
Mflv_4362|M.gilvum_PYR-GCK FRILLCLAEARPFVLLPK---FELHSWAEAVREHRPAAVSLVPAALRMVL
Mvan_1982|M.vanbaalenii_PYR-1 FRVLLCVAEARPFVLLPK---FELKQWADAVREHRPRAVSLVPAALRMVL
MSMEG_2217|M.smegmatis_MC2_155 FRVLLCIAEARPFVLLPK---FELQRWAAAVREHRPRAVSLVPAALRTVL
MAV_1580|M.avium_104 FRVLQCIADARPFVLLER---FELNAWAAAVRRHRPRAVSLVPAALRTVL
TH_3559|M.thermoresistible__bu YRVLQCIAAGRRFVLLER---FELQRWAEAVRTHRPRAVSLVPAALRMVL
MMAR_4025|M.marinum_M YRVLQCIAQARPFVLLDR---FELNAWAAAVRKHRPRTVSLVPAALRTVL
MUL_3890|M.ulcerans_Agy99 YRVLQCIAQARPFVLLDR---FELNARAAAVRKHRPRTVSLVPAALRTVL
MLBr_02661|M.leprae_Br4923 IAALLSTLASGGVVLLPARARFSARTFWDDIDAVAATWYTAAPAIHRILL
: . *: *. : .** : :
Mb0172|M.bovis_AF2122/97 TEQQAR---PRDLRLRVLSWGAAPAPDALLRQMSATFPETQILAAFGQTE
Rv0166|M.tuberculosis_H37Rv TEQQAR---PRDLRLRVLSWGAAPAPDALLRQMSATFPETQILAAFGQTE
MAB_4569c|M.abscessus_ATCC_199 AAQQAN---PRKVRLKTLSWGAAPATDVLLRTMSETFPDARILAAFGQTE
Mflv_4362|M.gilvum_PYR-GCK HSDLTR---DDLAGVRVVTSGTAPLSADDAEAFTAKFG-IPVLTSYAATE
Mvan_1982|M.vanbaalenii_PYR-1 HSDLTR---EDLDGIRVVTSGTAPLSADDADAFTEKFG-IPVLTSYAATE
MSMEG_2217|M.smegmatis_MC2_155 HSDLTR---EDLAGVRVVTSGTAPLSADDADAFTEKFG-IPVLTSYAATE
MAV_1580|M.avium_104 HSDLPR---ADLESIQVVTCGTAPLSADDADAFTEKYG-IPVLTSYAATE
TH_3559|M.thermoresistible__bu HSDLTR---EDLASIQAVTSGTAPLSADDADAFTEKFG-IPVLTSYAATE
MMAR_4025|M.marinum_M HSDLTR---ADLASVRTVTCGTAPLAPDDADAFTEKYG-IPVLTSYAATE
MUL_3890|M.ulcerans_Agy99 HSDPTR---ADLASVRTVTCGTAPLAPDDADAFTEKYG-IPVLTSYAATE
MLBr_02661|M.leprae_Br4923 ELASTQSFRSKRAKLRFIRSCSAPLTQETAQALREEFL-APVICAFGMTE
.. :: : :** . : : :: ::. **
Mb0172|M.bovis_AF2122/97 MSP----VTCMLLG--EDAIAKRGSVGRVIPTVAARVVDQNMNDVPVGEV
Rv0166|M.tuberculosis_H37Rv MSP----VTCMLLG--EDAIAKRGSVGRVIPTVAARVVDQNMNDVPVGEV
MAB_4569c|M.abscessus_ATCC_199 MSP----VTCMLMG--DDAIRKMGSVGRVIPTVSARVVDDNMNDVPVGEV
Mflv_4362|M.gilvum_PYR-GCK FGGGVAGWTLADHR--KYWQDKRGSVGRANPGARLRVVDDNRDPVPPGEP
Mvan_1982|M.vanbaalenii_PYR-1 FGGGVAGWTLKDHQ--RFWQHKRGSVGRANPGAQLRVVDDNGEPVPPGQQ
MSMEG_2217|M.smegmatis_MC2_155 FGGGVAGWTLPDHG--KYWKDKRGSVGRANPGAQLRVVDENGAPLGPDEP
MAV_1580|M.avium_104 FGGGVAGWTLADHR--RYWRVKRGSVGRANPGAALRVVAEDGTPLGPDEI
TH_3559|M.thermoresistible__bu FGGGVAGWTLADHR--RYWRTKRGSSGRATGGAQLRVVDEQGNPLGPDQP
MMAR_4025|M.marinum_M FGGGVAGWTLADHS--RYWHTKRGSVGRANPGTQLRIVDTEGAPVERASL
MUL_3890|M.ulcerans_Agy99 FGGGVAGWTLADHS--RYWHTKRGSVGRANPGTQLRIVDTEGAPVERASL
MLBr_02661|M.leprae_Br4923 ATHQVTTTNIKWFGQGENPTVTNGLVG-QSTGVQIRIVGSDGQPLPPDTV
. . * * . *:* : :
Mb0172|M.bovis_AF2122/97 GEIVYRAPTLMSCYWNNPEATAEAFAGGWFHSGDLVRMDSDGYVWVVDRK
Rv0166|M.tuberculosis_H37Rv GEIVYRAPTLMSCYWNNPEATAEAFAGGWFHSGDLVRMDSDGYVWVVDRK
MAB_4569c|M.abscessus_ATCC_199 GEIVYRAPTLMQGYWNNPEATADAFAGGWFHSGDLVRQDEDGFVWVVDRK
Mflv_4362|M.gilvum_PYR-GCK GLLEVIPAQLAD-------------TGEWMQTTDVARIDEDGFLWILGRA
Mvan_1982|M.vanbaalenii_PYR-1 GLLEVIPAQLAG-------------RSGWMQTTDLARIDEDGFVWILGRA
MSMEG_2217|M.smegmatis_MC2_155 GLLEVIPAQMGP-------------GSPWMRTTDLARIDADGFLWVLGRA
MAV_1580|M.avium_104 GLMEVKPGQLGP-------------DADWLRTTDMARIDADGFVWIVGRA
TH_3559|M.thermoresistible__bu GVLEVKPGQLGP-------------DADWLRTTDLARIDADGFLWILGRA
MMAR_4025|M.marinum_M GLLEVKPAQLGV-------------SAPWVRTTDVARIDADGFVWIVGRA
MUL_3890|M.ulcerans_Agy99 GLLEVKPAQLGV-------------SAPWVRTTDVARIDADGFVWIVGRA
MLBr_02661|M.leprae_Br4923 GEVWLRGSTVVRGYLGDPAITAANFTHGWLRTGDLGSLSVTGDLRIRGRI
* : : *.:: *: . * : : .*
Mb0172|M.bovis_AF2122/97 KDMIISGGENIYCAELENVLASHPDIAEVAVIGRADEKWGEVPIAVAAVT
Rv0166|M.tuberculosis_H37Rv KDMIISGGENIYCAELENVLASHPDIAEVAVIGRADEKWGEVPIAVAAVT
MAB_4569c|M.abscessus_ATCC_199 KDMIISGGENIYCAEVENALAEHEQIAEVAVIGRPHEKWGEVPVAVAAIH
Mflv_4362|M.gilvum_PYR-GCK DQAIIRGGFKVMPDDVRSALESHAAVAGAAVVGRPDPRLGETPVALVELR
Mvan_1982|M.vanbaalenii_PYR-1 DQAIIRGGFKVMPDDVKNALESHPAVAGASVVGRPDPRLGETPVALVQLR
MSMEG_2217|M.smegmatis_MC2_155 DQAIIRGGFKVMPDDVRAALESHPAVLGASVIGVADERLGETPVAMVELR
MAV_1580|M.avium_104 DQAIIRGGFKVMPDDVRAALESHPAVAGAAVVARPDPRLGETPVAMVELR
TH_3559|M.thermoresistible__bu DQAIIRGGFKIMPDDVRAALERHPAVRGAAVVGIPDARLGETPAALVELH
MMAR_4025|M.marinum_M DQAIIRGGFKVLPDDVGAALESHPAVAAAAVVGRSDQRLGQTPVAMVELR
MUL_3890|M.ulcerans_Agy99 DQAIIRGGFKVLPDDVRAALESHPAVAAAAVVGRSDQRLGQTPVAMVELR
MLBr_02661|M.leprae_Br4923 KELINRSGEKISPERVEGVLASHHNVMEVAVFGDPDKVYGETVTAVIVPR
.: * .* :: : .* * : .:*.. .. *:. *:
Mb0172|M.bovis_AF2122/97 N-DDLRIEDLGEFLTDRLARYKHPKALEIVDALPRNPAGKVLKTELRLRY
Rv0166|M.tuberculosis_H37Rv N-DDLRIEDLGEFLTDRLARYKHPKALEIVDALPRNPAGKVLKTELRLRY
MAB_4569c|M.abscessus_ATCC_199 G-AALTLAELDGFLTARLARYKHPKDLVIVDALPRNPSGKVLKTELRQRF
Mflv_4362|M.gilvum_PYR-GCK PGATASPEELSAYLQTRLARYEIPTALILTDAIPRTPSGKPDLTAVR---
Mvan_1982|M.vanbaalenii_PYR-1 PGMSAEPDELLNYLQKRLARYEIPGELVITDAIPRTASGKPDLTAVR---
MSMEG_2217|M.smegmatis_MC2_155 G--PATPDELAGYLQGRLARYEIPTRITIVDAIPRTPSGKADLRAVR---
MAV_1580|M.avium_104 --APSTTDALVEHLRERLARYEIPTEIAIVDALPRTPSGKADLAAVRGYF
TH_3559|M.thermoresistible__bu DGAAAGTDELTAFLRERLAPYEVPTEIAVVDALPRTPSGKADLGAVR---
MMAR_4025|M.marinum_M GSADIGGAELVEYLRTRLSGYEIPTQISIVAEIPRTASGKPDLGAIRHYF
MUL_3890|M.ulcerans_Agy99 GSADIGGAELVEYLRTRLSGYEIPTQISIVAEIPRTASGKPDLGAIRHYF
MLBr_02661|M.leprae_Br4923 EVIAPTPSELAVFCRDRLAAFEVPTRFQEASALPHTAKGSLDRRAVA---
* . **: :: * : . :*:.. *. :
Mb0172|M.bovis_AF2122/97 GACVNVERRSASAGFTERRENRQKL
Rv0166|M.tuberculosis_H37Rv GACVNVERRSASAGFTERRENRQKL
MAB_4569c|M.abscessus_ATCC_199 GIAD---------------------
Mflv_4362|M.gilvum_PYR-GCK -----ARLAQP--------------
Mvan_1982|M.vanbaalenii_PYR-1 -----SHFAQS--------------
MSMEG_2217|M.smegmatis_MC2_155 -----DHLESGG-------------
MAV_1580|M.avium_104 AERPTVLRDHAR-------------
TH_3559|M.thermoresistible__bu --RTLAPAGHDG-------------
MMAR_4025|M.marinum_M G-AAGSRTDHGG-------------
MUL_3890|M.ulcerans_Agy99 G-AAGSRTDHGG-------------
MLBr_02661|M.leprae_Br4923 -----EQFAHRG-------------