For questions or suggestions e-mail us at: ioerger@cs.tamu.edu
MPHNGGVTEGLTESFAAALHSYADRPCIEFDGRWYTGDEVVAYGAAIASLLETAGVPGDAPVGLIVRNRL PHAAAIAGFLAAGRTVAMIYSFQSAGAIAADVERLNLAAVVADAEDWTEPVVAAAARIGAAGVAISLGTP PVVAVPRLEHRDSSHAHAPAEPGVALKILTSGTTGPPKRQAIATHVLERTVFSVTAGEKADGDAPPEFAY WQFGGIGVCQLIAGLYNGRRVAMLERFSVEGWVDAVKRHRVTRAGVQPAVIRMLLDAKVPAEDLASLEFL ISASGPLDPETRDEFEARYRIPVRLAYGATEFAGSLCAWTPEMLDEFGKTKRESVGRALPDTELRIVDAE TGRERPPGERGLLEAKVGPLGPDWIRTTDIASIDEDGFVTLHGRADGAINRGGFKVQPEEVRRVLISHPG VRDACVVGVPDARLGEVPFAAIEVVPGADTPSDDELESLVRQELPVYAVPVAFTMVDALPRNPALKVSLP AVAALYGAV
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. gilvum PYR-GCK | Mflv_1694 | - | - | 100% (499) | AMP-dependent synthetase and ligase |
| M. gilvum PYR-GCK | Mflv_4362 | - | 2e-76 | 36.42% (497) | AMP-dependent synthetase and ligase |
| M. gilvum PYR-GCK | Mflv_0027 | - | 4e-36 | 33.80% (361) | AMP-dependent synthetase and ligase |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb1462c | fadD12 | 3e-30 | 27.18% (504) | acyl-CoA synthetase |
| M. tuberculosis H37Rv | Rv1427c | fadD12 | 3e-30 | 27.18% (504) | acyl-CoA synthetase |
| M. leprae Br4923 | MLBr_02546 | fadD2 | 2e-24 | 26.11% (471) | acyl-CoA synthetase |
| M. abscessus ATCC 19977 | MAB_4055c | - | 2e-29 | 26.79% (474) | putative acyl-CoA synthetase |
| M. marinum M | MMAR_4025 | - | 2e-66 | 34.40% (500) | long-chain fatty-acid CoA ligase |
| M. avium 104 | MAV_1580 | - | 2e-75 | 38.83% (479) | AMP-dependent synthetase and ligase |
| M. smegmatis MC2 155 | MSMEG_2217 | - | 3e-78 | 39.31% (496) | AMP-dependent synthetase and ligase |
| M. thermoresistible (build 8) | TH_3166 | - | 3e-86 | 38.87% (476) | PUTATIVE putative AMP-dependent synthetase and ligase |
| M. ulcerans Agy99 | MUL_3890 | - | 7e-66 | 34.60% (500) | long-chain fatty-acid CoA ligase |
| M. vanbaalenii PYR-1 | Mvan_5064 | - | 0.0 | 80.85% (496) | AMP-dependent synthetase and ligase |
CLUSTAL 2.0.9 multiple sequence alignment
Mb1462c|M.bovis_AF2122/97 -MRIRQAFG-------LIATMRRAGLIAPLRPDRYLRIVAAMRREGMGFT
Rv1427c|M.tuberculosis_H37Rv -MRIRQAFG-------LIATMRRAGLIAPLRPDRYLRIVAAMRREGMGFT
MLBr_02546|M.leprae_Br4923 MTKLRHYLERGAAEVHYLRSIFESGALGFEPPLTYAAMAVAMRKWG-DLA
Mflv_1694|M.gilvum_PYR-GCK --------------------------------------MPHNGGVTEGLT
Mvan_5064|M.vanbaalenii_PYR-1 --------------------------------------MPDNDTVTEGFT
MMAR_4025|M.marinum_M -------------------------------------------MSNHPLS
MUL_3890|M.ulcerans_Agy99 -------------------------------------------MSNHPLS
MAV_1580|M.avium_104 -------------------------------------------MPRHPLV
MSMEG_2217|M.smegmatis_MC2_155 --------------------------------------------MAHPLS
TH_3166|M.thermoresistible__bu --------------------------------------------------
MAB_4055c|M.abscessus_ATCC_199 --------------------------------MTFRSPLPDVSIPDCSVY
Mb1462c|M.bovis_AF2122/97 AGFAGAARRCPDRPGLID--ELGTLTWRQLDERGNALAAALQALPAGPPR
Rv1427c|M.tuberculosis_H37Rv AGFAGAARRCPDRPGLID--ELGTLTWRQLDERGNALAAALQALPAGPPR
MLBr_02546|M.leprae_Br4923 MLPAMPARRSPHRAALID--EEGTLTYKELDRAAHALANGLIAKGVRGGD
Mflv_1694|M.gilvum_PYR-GCK ESFAAALHSYADRPCIEF--DGRWYTGDEVVAYGAAIASLLETAGVPGDA
Mvan_5064|M.vanbaalenii_PYR-1 ERFAAALDGYGERPCIEY--DGRWYSGDEVMSYGAQIASVLHSAGVTGDA
MMAR_4025|M.marinum_M QRIADTLDLQPDARAVEY--DRAWLSWGQLGAATRRVAALAARQ---PSP
MUL_3890|M.ulcerans_Agy99 QRIAVTLDLQPDARAVEY--DRAWLSLGQLGAATRRVAALAAKQ---PSP
MAV_1580|M.avium_104 QRIADVLDLEPHGRAIEY--GGQWFSWAQLGATARQVAARTTG------T
MSMEG_2217|M.smegmatis_MC2_155 QRIADVLDLDPDAGAIQF--DGQWSCWGQIAALAGHIEKITGP------A
TH_3166|M.thermoresistible__bu -----VLALDPSAEAVEY--QGAWTDWKTVAHVAHAVESRLTAAGLGAGS
MAB_4055c|M.abscessus_ATCC_199 EYVFGDTGAEDSRIALIDGLTGAQTTLGELRSQIDATAAGLAERGFGIGD
: :
Mb1462c|M.bovis_AF2122/97 VVGIMCRNHRGFVDALLAVNRIGAHILLLNTSFAGPALAEVVTREGVDTV
Rv1427c|M.tuberculosis_H37Rv VVGIMCRNHRGFVDALLAVNRIGAHILLLNTSFAGPALAEVVTREGVDTV
MLBr_02546|M.leprae_Br4923 GIAILARNHRWFVIANYGCARVGARIILLNSEFSGPQIKEVSEREGAKVI
Mflv_1694|M.gilvum_PYR-GCK PVGLIVRNRLPHAAAIAGFLAAGRTVAMIYSFQSAGAIAADVERLNLAAV
Mvan_5064|M.vanbaalenii_PYR-1 PVGLIVRNRLQHAAAIAGFLAAGRTVAMIYSFQSPDAIARDIEQLNLAAV
MMAR_4025|M.marinum_M EIGILLRSRPAQMAAFLGVLLSGATVVTINPSRGDERTRADIAALELPLI
MUL_3890|M.ulcerans_Agy99 EIGILLRNRPAQMAAFLGVLLAGATVVTINPSRGDERTRADIAALELPLI
MAV_1580|M.avium_104 EVGMLLRNRPWQVAAFLGVLLGGGTVVVVNPSRGDERTRADLARLRLPLI
MSMEG_2217|M.smegmatis_MC2_155 RVGIMLRNHPVHVAALLGVLAAGGTVVVINPARGDDRTRADLEALALPVV
TH_3166|M.thermoresistible__bu PVGLILRNHPAMVAALLGVLLVNGCVVTINASSGDARLATDLEELRLPVV
MAB_4055c|M.abscessus_ATCC_199 VAAVFLPNCSAFAVVLHGILRAGGTASTVNVLYTAEELAKQLIDSKAQLI
.:: . . . . : :
Mb1462c|M.bovis_AF2122/97 VYDEEFSATVDRALAEKPQATRIVAWTDEDH-----DLTVEKLVAAHAGR
Rv1427c|M.tuberculosis_H37Rv VYDEEFSATVDRALAEKPQATRIVAWTDEDH-----DLTVEKLVAAHAGR
MLBr_02546|M.leprae_Br4923 IYDDEYTKVVSKAEPALGKLRALGVNPDEEEPPGSTDETLAELIARSSSA
Mflv_1694|M.gilvum_PYR-GCK VADAEDWT--EPVVAAAARIGAAGVAISLGT---PPVVAVPRLEHRDSSH
Mvan_5064|M.vanbaalenii_PYR-1 IADPEDWT--EPVVTGAARVGSAGVAISLSA---PTVAGVVGLERCDTAR
MMAR_4025|M.marinum_M AGSDEDLG--LLVTNTTDSAT-VSLSDLFDH---PGEPGDHTTR------
MUL_3890|M.ulcerans_Agy99 AGSDEDLG--LLVTNTTDSAT-VSLSDLFDH---PGEPGGHTTR------
MAV_1580|M.avium_104 IGEPDDLA--ALVT--DDTPT-MPISRLADP---PGP--AAPPA------
MSMEG_2217|M.smegmatis_MC2_155 VGLAEDLA--HLMPAHSPRTTRVAIAGLDTE---PVVTAAESPAT-----
TH_3166|M.thermoresistible__bu IGLGADWGRPHIVEAAAGALGLEVAADPASV---RTVTGRGSVGAGP---
MAB_4055c|M.abscessus_ATCC_199 FTVSPLLSRALEAAEIAGIDAAGVITVDPVEG--RLSLADIARPDLAPPQ
Mb1462c|M.bovis_AF2122/97 RPEHTGSHGKVILLTSGTTGTPKGA-RHSGGGIGTLKA---------ILD
Rv1427c|M.tuberculosis_H37Rv RPEHTGSHGKVILLTSGTTGTPKGA-RHSGGGIGTLKA---------ILD
MLBr_02546|M.leprae_Br4923 PAPKVRKRASIIILTSGTTGTPKGANRNTPASLAPLGG---------ILS
Mflv_1694|M.gilvum_PYR-GCK AHAPAEPGVALKILTSGTTGPPKRQAIATHVLERTVFS---------VTA
Mvan_5064|M.vanbaalenii_PYR-1 RHAPSEPGVALKILTSGTTGPPKRQSIRFNVLERTVFS---------VTA
MMAR_4025|M.marinum_M ---GAGADVAVRMLTSGTTGPPKRVDLSYDMLARSVMGPAPDR---DPAP
MUL_3890|M.ulcerans_Agy99 ---GAGADVAVRMLTSGTTGPPNRVDLSYDMLARSVMGPAPDR---DPAP
MAV_1580|M.avium_104 ---AAPRSVAVRMLTSGTTGPPKRIDLGYDMLARSVLGVEPGT---APAP
MSMEG_2217|M.smegmatis_MC2_155 --DAGGPGVAVWMLTSGTTGPPKRVDLTYDMLARSVMGREPEK---CPAP
TH_3166|M.thermoresistible__bu -FRPDRPGVAVEMLTSGTTGPPKRVPLSYNAFQHTVAAAGAHYGSGDNGQ
MAB_4055c|M.abscessus_ATCC_199 VTFDPATHLAVLPYSSGTTGKAKGVMLTHHNLVANIAQAK-------HLY
: :***** .: :
Mb1462c|M.bovis_AF2122/97 RTPWRAEEVTVIVAPMFHAWGFSQLVLASSLACTIVTRRRFDPEATLDLI
Rv1427c|M.tuberculosis_H37Rv RTPWRAEEVTVIVAPMFHAWGFSQLVLASSLACTIVTRRRFDPEATLDLI
MLBr_02546|M.leprae_Br4923 KVPFRAHEVTLLPAPMFHALGYLHATLAMFLGSTLVLRRRFKPATVLEDI
Mflv_1694|M.gilvum_PYR-GCK GEKADGDAPPEFAYWQFGGIGVCQLIAGLYNGRRVAMLERFSVEGWVDAV
Mvan_5064|M.vanbaalenii_PYR-1 GEKASGDAPPEFAYWQFGGIGVCQLIAGLYNGRRIAMLERFTVDGWVDAV
MMAR_4025|M.marinum_M TEPRRGVAIVNSPLVHIG--GVYRVLQCIAQARPFVLLDRFELNAWAAAV
MUL_3890|M.ulcerans_Agy99 TEPRRGVAIVNSPLVHIG--GVYRVLQCIAQARPFVLLDRFELNARAAAV
MAV_1580|M.avium_104 TEPRRGVAIVNSPLVHIG--GVFRVLQCIADARPFVLLERFELNAWAAAV
MSMEG_2217|M.smegmatis_MC2_155 TELRRGVAIVNSPLVHVG--GVFRVLLCIAEARPFVLLPKFELQRWAAAV
TH_3166|M.thermoresistible__bu VRLRSGVAIISSPLVHMS--GLFRTLLNICEGRRIALLERFRVDDFVDLV
MAB_4055c|M.abscessus_ATCC_199 GVQRGDRVLAVLPFFHIYG-LVVLLNVQLKLGAELVILPRFELDTFLGSI
. . .. :* :
Mb1462c|M.bovis_AF2122/97 DRHHATGLVVVPVMFDRIMDLPAEIRNRYDGRSLRFAAASGSRMRPDVVI
Rv1427c|M.tuberculosis_H37Rv DRHHATGLVVVPVMFDRIMDLPAEIRNRYDGRSLRFAAASGSRMRPDVVI
MLBr_02546|M.leprae_Br4923 EKHQATAMVVVPVMLSRILDTLENLETKSDLSSLRVVFVSGSQLGAELAT
Mflv_1694|M.gilvum_PYR-GCK KRHRVTRAGVQPAVIRMLLDAKVPAE---DLASLEFLISASGPLDPETRD
Mvan_5064|M.vanbaalenii_PYR-1 RRHRVTRCGVQPAVIRMLLDADVPKE---DLASLEFLISASGPLDPETRD
MMAR_4025|M.marinum_M RKHRPRTVSLVPAALRTVLHSDLTRA---DLASVRTVTCGTAPLAPDDAD
MUL_3890|M.ulcerans_Agy99 RKHRPRTVSLVPAALRTVLHSDPTRA---DLASVRTVTCGTAPLAPDDAD
MAV_1580|M.avium_104 RRHRPRAVSLVPAALRTVLHSDLPRA---DLESIQVVTCGTAPLSADDAD
MSMEG_2217|M.smegmatis_MC2_155 REHRPRAVSLVPAALRTVLHSDLTRE---DLAGVRVVTSGTAPLSADDAD
TH_3166|M.thermoresistible__bu VRHQPKAVSLVPSALAMVLDAGVPPE---VFSSVKVVTSGTAPLPVELQE
MAB_4055c|M.abscessus_ATCC_199 ANYRVDHVFVAPPVAVVLAKHPDVDK--YDVSCLRSVFSGAAPLDEQLGN
.:: : * : . :. . . : :
Mb1462c|M.bovis_AF2122/97 AFMDQFGDVIYNNYNATEAG-----MIATATPADLRTAPDTAGRPAEGTE
Rv1427c|M.tuberculosis_H37Rv AFMDQFGDVIYNNYNATEAG-----MIATATPADLRTAPDTAGRPAEGTE
MLBr_02546|M.leprae_Br4923 RALEELGPVIYNMYGSTEIA-----FATIAGPKDLQINSATVGPVVKGVK
Mflv_1694|M.gilvum_PYR-GCK EFEARYRIPVRLAYGATEFAGSLCAWTPEMLDEFGKTKRESVGRALPDTE
Mvan_5064|M.vanbaalenii_PYR-1 EFEQQYGIPIRLAYGATEFAGSLCAWTPELLQQYGESKRNSVGRALPDTE
MMAR_4025|M.marinum_M AFTEKYGIPVLTSYAATEFGGGVAGWTLADHSRYWHTKRGSVGRANPGTQ
MUL_3890|M.ulcerans_Agy99 AFTEKYGIPVLTSYAATEFGGGVAGWTLADHSRYWHTKRGSVGRANPGTQ
MAV_1580|M.avium_104 AFTEKYGIPVLTSYAATEFGGGVAGWTLADHRRYWRVKRGSVGRANPGAA
MSMEG_2217|M.smegmatis_MC2_155 AFTEKFGIPVLTSYAATEFGGGVAGWTLPDHGKYWKDKRGSVGRANPGAQ
TH_3166|M.thermoresistible__bu AFEERYDVAVLPSYGATEFAGGVAGWNLALHREWAKIKRGSVGRPQRGRE
MAB_4055c|M.abscessus_ATCC_199 AVAARLNCRVSQGYGMTELS----PVSHLIPPDRPDIPLNSVGIPVPNSE
. : * ** . :.* .
Mb1462c|M.bovis_AF2122/97 IRILD-QQFTEVPT-----GEVGTIYVRNDSQFDGYTSG----AAKDFHA
Rv1427c|M.tuberculosis_H37Rv IRILD-QQFTEVPT-----GEVGTIYVRNDSQFDGYTSG----AAKDFHA
MLBr_02546|M.leprae_Br4923 VKILD-DSGKEVPR-----GQVGRIFVGNAFPFEGYTGG----GGKQIID
Mflv_1694|M.gilvum_PYR-GCK LRIVDAETGRERPP-----GERGLLEAKVGPLG----------------P
Mvan_5064|M.vanbaalenii_PYR-1 LRVVDPETGGERAV-----GEQGLLEAKVAPIG----------------P
MMAR_4025|M.marinum_M LRIVD-TEGAPVER-----ASLGLLEVKPAQLGV--------------SA
MUL_3890|M.ulcerans_Agy99 LRIVD-TEGAPVER-----ASLGLLEVKPAQLGV--------------SA
MAV_1580|M.avium_104 LRVVA-EDGTPLGP-----DEIGLMEVKPGQLGP--------------DA
MSMEG_2217|M.smegmatis_MC2_155 LRVVD-ENGAPLGP-----DEPGLLEVIPAQMGP--------------GS
TH_3166|M.thermoresistible__bu VRIVSPEDGAELPP-----GTQGQIEVRTR------------------GG
MAB_4055c|M.abscessus_ATCC_199 NKIIDTETGDEIEIPAEGESAPGELLVRGPNVMAGYLGNEEATAATIEPD
::: * : .
Mb1462c|M.bovis_AF2122/97 GFMSSGDVGYLDENGRLFVVGRDDEMIVSGGENIYPIEVEKTLATHPDVA
Rv1427c|M.tuberculosis_H37Rv GFMSSGDVGYLDENGRLFVVGRDDEMIVSGGENIYPIEVEKTLATHPDVA
MLBr_02546|M.leprae_Br4923 GLLSSGDVGYFDEHDLLYISGRDDEMIVSGGENVFPAEVEDLISGHPEVV
Mflv_1694|M.gilvum_PYR-GCK DWIRTTDIASIDEDGFVTLHGRADGAINRGGFKVQPEEVRRVLISHPGVR
Mvan_5064|M.vanbaalenii_PYR-1 DWIRTTDIASIDEDGFVTLHGRADGAINRGGFKVLPEAVRRVLITHPDVR
MMAR_4025|M.marinum_M PWVRTTDVARIDADGFVWIVGRADQAIIRGGFKVLPDDVGAALESHPAVA
MUL_3890|M.ulcerans_Agy99 PWVRTTDVARIDADGFVWIVGRADQAIIRGGFKVLPDDVRAALESHPAVA
MAV_1580|M.avium_104 DWLRTTDMARIDADGFVWIVGRADQAIIRGGFKVMPDDVRAALESHPAVA
MSMEG_2217|M.smegmatis_MC2_155 PWMRTTDLARIDADGFLWVLGRADQAIIRGGFKVMPDDVRAALESHPAVL
TH_3166|M.thermoresistible__bu EWVRTTDLGRLDSDGFLFVDGRTDDAIIRGGFKISPADIANALRSHPAVR
MAB_4055c|M.abscessus_ATCC_199 GFLHTGDIAVVRADGVVTIVDRLKELIKYKGYQVPPAELEALLLTHPGIG
: : *:. . .. : : .* . * * :: * : : ** :
Mb1462c|M.bovis_AF2122/97 EAAVIGVDDQQYGQRLAAFVVLK-PGVSATPETLKQHVRDNLANYKVPRD
Rv1427c|M.tuberculosis_H37Rv EAAVIGVDDQQYGQRLAAFVVLK-PGVSATPETLKQHVRDNLANYKVPRD
MLBr_02546|M.leprae_Br4923 EATAIGVDDKEWGARLRAFVVKK-QDATIDEDAIKAYVRDHLARYKVPRE
Mflv_1694|M.gilvum_PYR-GCK DACVVGVPDARLGEVPFAAIEVVPGADTPSDDELESLVRQELPVYAVPVA
Mvan_5064|M.vanbaalenii_PYR-1 DACVVGVPDARLGQVPFAAIEVSAGTAAPSDGELRELVRQSLPVYNVPVA
MMAR_4025|M.marinum_M AAAVVGRSDQRLGQTPVAMVELR-GSADIGGAELVEYLRTRLSGYEIPTQ
MUL_3890|M.ulcerans_Agy99 AAAVVGRSDQRLGQTPVAMVELR-GSADIGGAELVEYLRTRLSGYEIPTQ
MAV_1580|M.avium_104 GAAVVARPDPRLGETPVAMVELR-APSTT--DALVEHLRERLARYEIPTE
MSMEG_2217|M.smegmatis_MC2_155 GASVIGVADERLGETPVAMVELR-GPATP--DELAGYLQGRLARYEIPTR
TH_3166|M.thermoresistible__bu DAGVTGLPDPRLGAVPVAAVELA-GGAQAEPDELLDFLRQRLTRYQVPAR
MAB_4055c|M.abscessus_ATCC_199 DAAVIGVPDPSSGEIPKAFVVRT--DDDLTDEAVMAFVEQKVAPHKRIRQ
* . . * * * : : :. :. :
Mb1462c|M.bovis_AF2122/97 IAVLDELPRGITGKILRTELQSRVGS----------
Rv1427c|M.tuberculosis_H37Rv IAVLDELPRGITGKILRTELQSRVGS----------
MLBr_02546|M.leprae_Br4923 VIFLEELPRNPTGKVLKRELRDFEVH----------
Mflv_1694|M.gilvum_PYR-GCK FTMVDALPRNPALKVSLPAVAALYGAV---------
Mvan_5064|M.vanbaalenii_PYR-1 FAAVEELPRNPALKVSLPAVAALYSGHTGD------
MMAR_4025|M.marinum_M ISIVAEIPRTASGKPDLGAIRHYFGAAGSRT-DHGG
MUL_3890|M.ulcerans_Agy99 ISIVAEIPRTASGKPDLGAIRHYFGAAGSRT-DHGG
MAV_1580|M.avium_104 IAIVDALPRTPSGKADLAAVRGYFAERPTVLRDHAR
MSMEG_2217|M.smegmatis_MC2_155 ITIVDAIPRTPSGKADLRAVRDHLESGG--------
TH_3166|M.thermoresistible__bu LLVLDELPRTPSLKVSQPALRELFEQEPT-------
MAB_4055c|M.abscessus_ATCC_199 VEFIDAIPKSAAGKILRKDLRARI------------
. : :*: : * :