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VADAESLAAHGSRVAGKGISVKDVVIVGGGPTGAMLAGELRLYEVDVMVLDKDIEPTKVVRSLGLHARSI EIMDQRGLLDRFLEVGRRHPVGGYFAGITKAPPRTLDTAHPYVLGIPQPVTDRILVEHAVASGAVLRRGC EAVGLAQDDDGVSVTLADGTQVRARYVVGCDGGRSTVRKLMGVGFPGEPSRVDTLIGEMQVGVTPEQVTA VVAEVRKMQLRFGAGPLGDGLYRVMVPAAEVVEDRSATPTLDEFRQQLRATAGTDLGVHSPRWLSRFGDG TRLADRYRTGRVLLAGDAAHVHPPVGGQGLNLGVQDAFNLGWKLAASINGWAPDGLLDSYEAERRPVAAA VLDNTRAQMELLSPEPGPQAVRRLLAQLMAFEEVNRYLTEMITATGIRYDFGAEDDLVGRRLRDIRLRRG RLYELTRTGRGLLLDQTGQLSAAGWSDRVDHVVDVSDELDVPAVLLRPDGHIAWVGDEQGDLEGCLVRWF GVPA
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. smegmatis MC2 155 | MSMEG_2252 | - | - | 100% (494) | flavin-type hydroxylase |
| M. smegmatis MC2 155 | MSMEG_1649 | - | 1e-73 | 36.93% (501) | FAD dependent oxidoreductase |
| M. smegmatis MC2 155 | MSMEG_1499 | - | 2e-47 | 31.01% (503) | oxygenase |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb1780 | - | 1e-13 | 26.20% (313) | hypothetical protein Mb1780 |
| M. gilvum PYR-GCK | Mflv_5009 | - | 5e-50 | 32.66% (493) | monooxygenase, FAD-binding |
| M. tuberculosis H37Rv | Rv1751 | - | 1e-13 | 26.06% (307) | hypothetical protein Rv1751 |
| M. leprae Br4923 | MLBr_02276 | - | 6e-09 | 30.72% (166) | putative FAD-linked oxidoreductase |
| M. abscessus ATCC 19977 | MAB_0857 | - | 1e-102 | 44.22% (493) | hypothetical protein MAB_0857 |
| M. marinum M | MMAR_4271 | - | 7e-94 | 43.43% (495) | hypothetical protein MMAR_4271 |
| M. avium 104 | MAV_2736 | - | 5e-97 | 43.14% (503) | hypothetical protein MAV_2736 |
| M. thermoresistible (build 8) | TH_1880 | - | 0.0 | 70.50% (478) | flavin-type hydroxylase |
| M. ulcerans Agy99 | MUL_3663 | - | 5e-13 | 24.39% (410) | hypothetical protein MUL_3663 |
| M. vanbaalenii PYR-1 | Mvan_2422 | - | 3e-91 | 42.13% (489) | hypothetical protein Mvan_2422 |
CLUSTAL 2.0.9 multiple sequence alignment
Mb1780|M.bovis_AF2122/97 MIATMPSMARRSRHDNKITTPAVDCLTIERLDSPASGAPQVTPYARALMG
Rv1751|M.tuberculosis_H37Rv MIATMPSMARRSRHDNKITTPAVDCLTIERLDSPASGAPQVTPYARALMG
MUL_3663|M.ulcerans_Agy99 -------------------------------------MPQPS--------
MSMEG_2252|M.smegmatis_MC2_155 ---------------MADAESLAAHGSRVAG----------------KGI
TH_1880|M.thermoresistible__bu --------------------------------------------------
MAV_2736|M.avium_104 ---------------MRGVRFSE--RSVRG--------------------
Mvan_2422|M.vanbaalenii_PYR-1 ---------------MTTTRHKDTHKDTREDTREDTRDDSRDDGGDPNGD
MMAR_4271|M.marinum_M ---------------MAD--------------------------------
MAB_0857|M.abscessus_ATCC_1997 ---------------MAE--------------------------------
Mflv_5009|M.gilvum_PYR-GCK ---------------MPGAHR-----------------------------
MLBr_02276|M.leprae_Br4923 ----------------------------------------------MRVE
Mb1780|M.bovis_AF2122/97 ETTTCAIIGGGPAGMVLGLLLARAGVQVTLLEKHGDFLRD-FRGDTVHPT
Rv1751|M.tuberculosis_H37Rv ETTTCAIIGGGPAGMVLGLLLARAGVQVTLLEKHGDFLRD-FRGDTVHPT
MUL_3663|M.ulcerans_Agy99 ETTSVLVAGGGPAGMVLGLLLARAGVDVTVMEKHADFLRD-SRGDTVHAS
MSMEG_2252|M.smegmatis_MC2_155 SVKDVVIVGGGPTGAMLAGELRLYEVDVMVLDKDIEPTKV-VRSLGLHAR
TH_1880|M.thermoresistible__bu -VYDVIVVGGGPTGMMLAGELRLHGVRVLVVEKNSEPPAH-VRSLGLHVR
MAV_2736|M.avium_104 --HAVLVAGAGPTGLMLAGELGLAGVDVALVERRADQQLIGSRAGGLHAR
Mvan_2422|M.vanbaalenii_PYR-1 TDHAVVVGGGGPTGMMTAAELALAGVDVAVVERRTTPEITGSRAGGLLSR
MMAR_4271|M.marinum_M --HAVVVVGAGPTGLMLAGELALAGVDVAVVERRVSQDLVGSRAGGLHSR
MAB_0857|M.abscessus_ATCC_1997 --HAVVVAGAGPTGLMLAGELALAGIDVALLERRGSHEEVGSRAAGLHSR
Mflv_5009|M.gilvum_PYR-GCK --SDVVVVGAGPTGLTLACCLRLHGVSVRVLDAAAG-PATTSRANFLHAR
MLBr_02276|M.leprae_Br4923 TSADVVVVGAGPAGSAAATWAARAGNDVIVIDSVCFPRDK-ACGDGLTPR
: *.**:* . * ::: . :
Mb1780|M.bovis_AF2122/97 TMRLLDELGLWERFAALPYSEVRTATLHSNGRAVTYIDFERLHQPYPYVA
Rv1751|M.tuberculosis_H37Rv TMRLLDELGLWERFAALPYSEVRTATLHSNGRAVTYIDFERLHQPYPYVA
MUL_3663|M.ulcerans_Agy99 TLRLLDELGLGSQFAQIPHRLIDTISMKVQGQPVD-LDLKHLPGAHQHIA
MSMEG_2252|M.smegmatis_MC2_155 SIEIMDQRGLLDRFLEV-GRRHPVGGYFAGITKAPPRTLD----TAHPYV
TH_1880|M.thermoresistible__bu SIEMLDQRGLLDRFLAH-GQRHPVGGFFAAIAK-PPAELD----TAHGYV
MAV_2736|M.avium_104 TLEILDQRGIVDRFLAE-GQVAQVAG-FAQIRL-DISDFP----TRHPYG
Mvan_2422|M.vanbaalenii_PYR-1 TLEILDQRGVVDRFLSA-GETMQVHG-FAQVRL-DVSDLP----TRHPYG
MMAR_4271|M.marinum_M TIEVLDQRGIADRFLAQ-GTATQVAR-FAAIPL-DISDFP----TRHNYG
MAB_0857|M.abscessus_ATCC_1997 TIEVFDQRGIAERFLAQ-GRTGQVTH-FSGIFM-SIEDFP----TRHPYA
Mflv_5009|M.gilvum_PYR-GCK GSEVLDRMGALGTLPQE-SLRALRITNYLGDRPLATLEFGDPGMRTAAPP
MLBr_02276|M.leprae_Br4923 AVAELKRLGLDDWLNTRIRHRGLRMSGFGGEVEVDWPGPS-----FPSTS
:.. * :
Mb1780|M.bovis_AF2122/97 MVPQWDLLNLLAEAAQAEPSFTLRMKTEVTGLLR----EGGKVTGVRYQG
Rv1751|M.tuberculosis_H37Rv MVPQWDLLNLLAEAAQAEPSFTLRMKTEVTGLLR----EGGKVTGVRYQG
MUL_3663|M.ulcerans_Agy99 LVPQWDFLELLAAAAEAEPTFRLLRSTEVTGVLWGDGPDPGRVVSATYRA
MSMEG_2252|M.smegmatis_MC2_155 LGIPQPVTDRILVEHAVASGAVLRRGCEAVGLAQ-------DDDGVSVTL
TH_1880|M.thermoresistible__bu LGIRQPVTDRLLAEWAAERGAVIRRGCAVVGLDQ-------DAAGITVEL
MAV_2736|M.avium_104 LALWQNHIERILADWVGELGVPIYRGHRIATCTQ-------DDTGVDVVA
Mvan_2422|M.vanbaalenii_PYR-1 LALWQKHIERILAEWVDELAVPVYRGREVVGVRQ-------DGAGVDIEL
MMAR_4271|M.marinum_M LALWQNRIERILAEWVAELRVRIYRGCEVTDITQ-------DDNGVDLTL
MAB_0857|M.abscessus_ATCC_1997 LGLLQHYTEKTLAAWIDELGVPIYYNAEAVGLGQ-------DDVGVDVAL
Mflv_5009|M.gilvum_PYR-GCK MVVSQAKVEAALRARLADLDVEPEWGRKVIDVRE-------DADGVVADL
MLBr_02276|M.leprae_Br4923 SAVPRLELDDRIRKVAENSGARMLLGTKAVSVYHD-----SSRRAASVTL
: .
Mb1780|M.bovis_AF2122/97 AEG-PGELRAELTVACDGRWSIARHEAGLKAREFP--VNFDVWWFKLPRE
Rv1751|M.tuberculosis_H37Rv AEG-PGELRAELTVACDGRWSIARHEAGLKAREFP--VNFDVWWFKLPRE
MUL_3663|M.ulcerans_Agy99 QSGETKHMHADLTVACDGRGSTVRSALGMATRSFG--APMDVWWFRLPRR
MSMEG_2252|M.smegmatis_MC2_155 ADG--TQVRARYVVGCDGGRSTVRKLMGVGFPGEPSRVDTLIGEMQVGVT
TH_1880|M.thermoresistible__bu ADG--SVQRARYLVGCDGGRSTVRKLAGIGFPGEPARSETLLGEMSLSAD
MAV_2736|M.avium_104 SDG--RTLRGQYLVGCDGGRSVIRKAAGIDFPGWEATTSYLLAEAEMDWR
Mvan_2422|M.vanbaalenii_PYR-1 SDG--RVLRAGYLVGCDGGRSLIRKSAGIEFPGWEPTTSYLLAEVEIAPE
MMAR_4271|M.marinum_M SDG--PPLRADYVVGCDGGRSLVRRAVGIDFPGWDPTTSSLIAEVEVAVG
MAB_0857|M.abscessus_ATCC_1997 ADG--STLRGQYLVGCDGGRSTIRKAAGIGFPGHDPSCSAMLVDAEIT--
Mflv_5009|M.gilvum_PYR-GCK ADG--ARVRGQWLVGCDGTTSVVRRQTGIEFPGVRLSERFLLADIHLDWA
MLBr_02276|M.leprae_Br4923 ADG--TEVGCRQLIIADGARSPLGRKLGRSWHQKTVYGVAARGYLTTPRS
.* : .** * *
Mb1780|M.bovis_AF2122/97 GDAEFSFLPRFSPGKGLGVIPREGYFQIAYLGPKGTDAQLRERG--IEEF
Rv1751|M.tuberculosis_H37Rv GDAEFSFLPRFSPGKGLGVIPREGYFQIAYLGPKGTDAQLRERG--IEEF
MUL_3663|M.ulcerans_Agy99 PDDPTGLAGIFNSGHGGIAIDRGDYYQIAYIIPKGSDAQLRAQG--IEAL
MSMEG_2252|M.smegmatis_MC2_155 PEQVTAVVAEVRKMQLRFGAGPLG---DGLYRVMVPAAEVVEDRS-ATPT
TH_1880|M.thermoresistible__bu PATVAAVVAEVRKTQLRFGAMPLG---DGVYRVIVPAEGVAGDR--AEPT
MAV_2736|M.avium_104 TGQPPQWG-IHHDSLGVHALSETEP--AGGVRVLVTERQLRSAA---EPN
Mvan_2422|M.vanbaalenii_PYR-1 DGREPEWG-IRRDALGVHALSRSD---DGPVRIMVTEQDLRRSG---VPD
MMAR_4271|M.marinum_M SESGPNLGEVRHDALGVHGFTRLPD--GGPLRVVVTERQLDAGG---EPS
MAB_0857|M.abscessus_ATCC_1997 -EQPLEFGLLRKPGQAFLGILPFE---EGWCRLAFSTGLDRLGV---EPT
Mflv_5009|M.gilvum_PYR-GCK VDRAGTTGWIHPDGVVGAMPMPSTDGRDDLWRLFVYDPSLDRKPT-DSEI
MLBr_02276|M.leprae_Br4923 DDLWLTSHLELRSPDGAVLPGYGWIFPLGNGEVNIGVGALSTSKRPADLA
Mb1780|M.bovis_AF2122/97 RRDVSELLPEATASVAALAS--TDEVKHLNVKVNRLRRWHIDGLLCIGDA
Rv1751|M.tuberculosis_H37Rv RRDVSELLPEATASVAALAS--MDEVKHLNVKVNRLRRWHIDGLLCIGDA
MUL_3663|M.ulcerans_Agy99 HRALADLMPWLADRVDALKS--FDDVKLLDVQLNRLRRWYCDGALCIGDA
MSMEG_2252|M.smegmatis_MC2_155 LDEFRQQLRATAGTDLGVHS--PRWLSRFGDGTRLADRYRTGRVLLAGDA
TH_1880|M.thermoresistible__bu LDEFRRRLKAFAGTDFGAHT--PRWLSRFGDATRLAERYRAGRVFLAGDA
MAV_2736|M.avium_104 LDDLRQALIAVYRTDYGIHS--PRWISRFTDAARQAARYRDRRVLLAGDA
Mvan_2422|M.vanbaalenii_PYR-1 LAELREALVTVYGTDFGIHS--PTSISRFTDTARQAVAYRSGRILLVGDA
MMAR_4271|M.marinum_M LGDLSAALTAVYGTDYGIHS--PTWISRFTDLTRQAATYRQGRVLIAGDA
MAB_0857|M.abscessus_ATCC_1997 LEEAKEALIAIAGTDFGIHN--PRWISRFSDMTRQADRYRDGRVFLAGDS
Mflv_5009|M.gilvum_PYR-GCK LDRISELVPYRTGRRVKIGD--AEWLSMFTVHRRLADTYRRGRVLIAGDA
MLBr_02276|M.leprae_Br4923 LRSLISYYTDLRRHEWGFSGQPRAVSSALLPMGGAVSGVAGPNWMLIGDA
. : : **:
Mb1780|M.bovis_AF2122/97 AHAMSPVAGVGINLAVQDAVAAATILAEPLREHRVSSRHLAAVRRRRAFP
Rv1751|M.tuberculosis_H37Rv AHAMSPVAGVGINLAVQDAVAAATILAEPLREHRVSSRHLAAVRRRRAFP
MUL_3663|M.ulcerans_Agy99 AHAMSPVGGVGINLALADAVATARMLAPSLRVGRVSTGQLARVQARRWAP
MSMEG_2252|M.smegmatis_MC2_155 AHVHPPVGGQGLNLGVQDAFNLGWKLAASINGW--APDGLLDSYEAERRP
TH_1880|M.thermoresistible__bu AHVHPPAGGQGLNLGIQDAFNLGWKLAAAVGGW--APDGLLDTYHAERHP
MAV_2736|M.avium_104 AHIHHPVGGQGLNTGVQDAVNLGWKLALVVGGA--SPPALLDTYHAERHP
Mvan_2422|M.vanbaalenii_PYR-1 AHVHHPVGGQGLNTGMQDAVNLGWKLAQVVQGW--SPDTLLDTYHDERQP
MMAR_4271|M.marinum_M AHVHDPVGGQGLNTGVQDAVNLGWKLAQVVNRR--SPESLLDTYHAERHP
MAB_0857|M.abscessus_ATCC_1997 AHIHFPFGGQGLNTGIQDAVNLGWKLAAVIKGH--APDALLDTYHLERHP
Mflv_5009|M.gilvum_PYR-GCK AHVHAPFGGQGMLTGIGDAENVGFKLALVVRGL--AADDLLDTYEAERRP
MLBr_02276|M.leprae_Br4923 AACVNPLNGEGIDYGLETGRLAAELLDLRDLSY--VWPSLLQQHYGRGFS
* * * *: .: . . * * . .
Mb1780|M.bovis_AF2122/97 TAVTQAVQRVLHRRLLGPLLQGRDPTPPAALLGLVERLPWLSAVPAYFVG
Rv1751|M.tuberculosis_H37Rv TAVTQAVQRVLHRRLLGPLLQGRDPTPPAALLGLVERLPWLSAVPAYFVG
MUL_3663|M.ulcerans_Agy99 TAFLQGIQRAIHAAIVAVAVTGGPPQPPPVVR-LVSRSVPVRRIMGYLVA
MSMEG_2252|M.smegmatis_MC2_155 VAAAVLDNTRAQMELLSP-EPGPQAVRRLLA-QLMAFEEVNRYLTEMITA
TH_1880|M.thermoresistible__bu VAADVLNNTRAQMELMSP-EPGPRAVRWLLA-ELMDFEEVNRYLTEKITA
MAV_2736|M.avium_104 VGERVLRNAIAQMALLRP-DERTRALRDLVT-ELLGMDEPRKRFAAMISG
Mvan_2422|M.vanbaalenii_PYR-1 VGARVLRNAVAQMALLRP-DDRTTVLHGVVS-DMLDMDEPRRRFAAMMSG
MMAR_4271|M.marinum_M VAARVLRTTMAQVALRRL-DYRKKALSDTIS-ELLMMDEPRKRFAAMMSG
MAB_0857|M.abscessus_ATCC_1997 VAERVLHNTMAQTPLSEA-GPRIDALRDIVA-DLLAMDEPRKRIAGMMSG
Mflv_5009|M.gilvum_PYR-GCK LATQVLRGTSAITRVNVAGNPIVRFLRDRVAPRVFGLPAVQRWTTYTASQ
MLBr_02276|M.leprae_Br4923 VARRLALLLTFKRCLPAIGPSAMRSTSLITIAVRVMANLVTDDDADWVAR
. : .
Mb1780|M.bovis_AF2122/97 VGVRPEH--------APAFARRGPGNRKGP--------------------
Rv1751|M.tuberculosis_H37Rv VGVRPEH--------APAFARRGPGNRKGP--------------------
MUL_3663|M.ulcerans_Agy99 IGPLPEH--------APKYPRRAR--------------------------
MSMEG_2252|M.smegmatis_MC2_155 TGIRYD-----FGAEDDLVGRRLRDIRLRRG----RLYELTRTGRGLLLD
TH_1880|M.thermoresistible__bu IGVRYHT----MGDGHGLLGRRMRDIPLTGG----RLYARMHAGRGVLLD
MAV_2736|M.avium_104 LDVRYD-----LGPGHPLLGRRMPDLDLVTADGASRVFALLRNARPVLLN
Mvan_2422|M.vanbaalenii_PYR-1 LDIHYD-----LGDGHPLLGLRMPDLDLETAHGPTRVSALLRQARPVLLD
MMAR_4271|M.marinum_M LDIRYH-----LGGGHPLLGRRMPDLDLTTADGPRRVFTHLHAARPVLLN
MAB_0857|M.abscessus_ATCC_1997 LDIHYM-----LGDGHQLLGRRMPDLDVVTADGAVRVYEHLHTARPVLFN
Mflv_5009|M.gilvum_PYR-GCK LWVSYRNGPLGGRGSKPRPGDRIDDAPCSRPDGTATRLHGELGGRWALLL
MLBr_02276|M.leprae_Br4923 VWRSGG--------WASWLVDRRPPFS-----------------------
*
Mb1780|M.bovis_AF2122/97 --------------------------------------------------
Rv1751|M.tuberculosis_H37Rv --------------------------------------------------
MUL_3663|M.ulcerans_Agy99 --------------------------------------------------
MSMEG_2252|M.smegmatis_MC2_155 QTG--QLSAAGWSDRVDHVVD----------VSDELDVPAVLLRPDGHIA
TH_1880|M.thermoresistible__bu RTG--TLSVSGWADRVDHIVD----------PGAALDVPAVLLRPDGHVA
MAV_2736|M.avium_104 FAGAGRRSVAGWADRVRVVDAKCVADWELPVLGRVSAPPAVLIRPDGHVA
Mvan_2422|M.vanbaalenii_PYR-1 LGAPDAVDLTGWEARVRMVRANAATVWELPVIGEVPAPAAVLIRPDGHVA
MMAR_4271|M.marinum_M LAEPGRFDIAGWRHRVLLIDAKYVGVWELPVLGAIAAPAAALIRPDGYVA
MAB_0857|M.abscessus_ATCC_1997 FGARQILNVEGWEDRVHVVDAKYSGIWELPVLGEVSAPDAVLVRPDGYVA
Mflv_5009|M.gilvum_PYR-GCK PRGVPAVGAAAVRGPAGYLADH-------VVTLHHDRDDVMLVRPDAHLA
MLBr_02276|M.leprae_Br4923 --------------------------------------------------
Mb1780|M.bovis_AF2122/97 -----------------------------------
Rv1751|M.tuberculosis_H37Rv -----------------------------------
MUL_3663|M.ulcerans_Agy99 -----------------------------------
MSMEG_2252|M.smegmatis_MC2_155 WVGDE---QGDLEGCLVRWFGVPA-----------
TH_1880|M.thermoresistible__bu WVGADHPGQQDLTARLSRWFGAPA-----------
MAV_2736|M.avium_104 WAGDP--NDPGLHDALTTWCGPPAAGG--------
Mvan_2422|M.vanbaalenii_PYR-1 WVAGD--SAAAPAEAAARWFGAPTGRR--------
MMAR_4271|M.marinum_M WVGDDL-TDRGLFDALTAWFGRPAVPSPRLRASAP
MAB_0857|M.abscessus_ATCC_1997 WVGEG--SADGLRGALTRWVGSPSRA---------
Mflv_5009|M.gilvum_PYR-GCK WRGAHD-DPTGLDRWLATALHPGGTQ---------
MLBr_02276|M.leprae_Br4923 -----------------------------------