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ADHAVVVVGAGPTGLMLAGELALAGVDVAVVERRVSQDLVGSRAGGLHSRTIEVLDQRGIADRFLAQGTA TQVARFAAIPLDISDFPTRHNYGLALWQNRIERILAEWVAELRVRIYRGCEVTDITQDDNGVDLTLSDGP PLRADYVVGCDGGRSLVRRAVGIDFPGWDPTTSSLIAEVEVAVGSESGPNLGEVRHDALGVHGFTRLPDG GPLRVVVTERQLDAGGEPSLGDLSAALTAVYGTDYGIHSPTWISRFTDLTRQAATYRQGRVLIAGDAAHV HDPVGGQGLNTGVQDAVNLGWKLAQVVNRRSPESLLDTYHAERHPVAARVLRTTMAQVALRRLDYRKKAL SDTISELLMMDEPRKRFAAMMSGLDIRYHLGGGHPLLGRRMPDLDLTTADGPRRVFTHLHAARPVLLNLA EPGRFDIAGWRHRVLLIDAKYVGVWELPVLGAIAAPAAALIRPDGYVAWVGDDLTDRGLFDALTAWFGRP AVPSPRLRASAP*
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. marinum M | MMAR_4271 | - | - | 100% (503) | hypothetical protein MMAR_4271 |
| M. marinum M | MMAR_3439 | - | 6e-72 | 37.45% (510) | hydroxylase |
| M. marinum M | MMAR_4777 | mhpA | 4e-33 | 30.16% (514) | hydroxylase |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb1780 | - | 7e-16 | 27.55% (363) | hypothetical protein Mb1780 |
| M. gilvum PYR-GCK | Mflv_5009 | - | 2e-48 | 34.59% (529) | monooxygenase, FAD-binding |
| M. tuberculosis H37Rv | Rv1751 | - | 9e-16 | 27.55% (363) | hypothetical protein Rv1751 |
| M. leprae Br4923 | - | - | - | - | - |
| M. abscessus ATCC 19977 | MAB_3483 | - | 1e-173 | 64.17% (494) | hypothetical protein MAB_3483 |
| M. avium 104 | MAV_2736 | - | 0.0 | 68.30% (489) | hypothetical protein MAV_2736 |
| M. smegmatis MC2 155 | MSMEG_2252 | - | 7e-94 | 43.43% (495) | flavin-type hydroxylase |
| M. thermoresistible (build 8) | TH_1880 | - | 1e-103 | 45.33% (492) | flavin-type hydroxylase |
| M. ulcerans Agy99 | MUL_3663 | - | 1e-14 | 28.53% (333) | hypothetical protein MUL_3663 |
| M. vanbaalenii PYR-1 | Mvan_2422 | - | 1e-175 | 63.60% (489) | hypothetical protein Mvan_2422 |
CLUSTAL 2.0.9 multiple sequence alignment
MMAR_4271|M.marinum_M -------------------------------------------------M
MAV_2736|M.avium_104 ---------------------------------------MRGVRFSERSV
Mvan_2422|M.vanbaalenii_PYR-1 ---------------MTTTRHKDTHKDTREDTREDTRDDSRDDGGDPNGD
MAB_3483|M.abscessus_ATCC_1997 -------------------------------------------------M
MSMEG_2252|M.smegmatis_MC2_155 -------------------------------MADAESLAAHGSRVAGKGI
TH_1880|M.thermoresistible__bu --------------------------------------------------
Mflv_5009|M.gilvum_PYR-GCK ----------------------------------------------MPGA
Mb1780|M.bovis_AF2122/97 MIATMPSMARRSRHDNKITTPAVDCLTIERLDSPASGAPQVTPYARALMG
Rv1751|M.tuberculosis_H37Rv MIATMPSMARRSRHDNKITTPAVDCLTIERLDSPASGAPQVTPYARALMG
MUL_3663|M.ulcerans_Agy99 -------------------------------------MPQPS--------
MMAR_4271|M.marinum_M ADHAVVVVGAGPTGLMLAGELALAGVDVAVVERRVSQDLVGSRAGGLHSR
MAV_2736|M.avium_104 RGHAVLVAGAGPTGLMLAGELGLAGVDVALVERRADQQLIGSRAGGLHAR
Mvan_2422|M.vanbaalenii_PYR-1 TDHAVVVGGGGPTGMMTAAELALAGVDVAVVERRTTPEITGSRAGGLLSR
MAB_3483|M.abscessus_ATCC_1997 TEHAVVIAGGGPTGLMLAGELALAGADVAIVERRTTHELDGSRAAGMTSR
MSMEG_2252|M.smegmatis_MC2_155 SVKDVVIVGGGPTGAMLAGELRLYEVDVMVLDKDIEPTKV-VRSLGLHAR
TH_1880|M.thermoresistible__bu -VYDVIVVGGGPTGMMLAGELRLHGVRVLVVEKNSEPPAH-VRSLGLHVR
Mflv_5009|M.gilvum_PYR-GCK HRSDVVVVGAGPTGLTLACCLRLHGVSVRVLDAAAG-PATTSRANFLHAR
Mb1780|M.bovis_AF2122/97 ETTTCAIIGGGPAGMVLGLLLARAGVQVTLLEKHGDFLRD-FRGDTVHPT
Rv1751|M.tuberculosis_H37Rv ETTTCAIIGGGPAGMVLGLLLARAGVQVTLLEKHGDFLRD-FRGDTVHPT
MUL_3663|M.ulcerans_Agy99 ETTSVLVAGGGPAGMVLGLLLARAGVDVTVMEKHADFLRD-SRGDTVHAS
: *.**:* . * . * ::: *. :
MMAR_4271|M.marinum_M TIEVLDQRGIADRFLAQGTATQ-----VARFAAIPLD--ISDFPTRHNYG
MAV_2736|M.avium_104 TLEILDQRGIVDRFLAEGQVAQ-----VAGFAQIRLD--ISDFPTRHPYG
Mvan_2422|M.vanbaalenii_PYR-1 TLEILDQRGVVDRFLSAGETMQ-----VHGFAQVRLD--VSDLPTRHPYG
MAB_3483|M.abscessus_ATCC_1997 TLEVLDQRGILDRFTPQGFSAP-----VAHFSALLLD--MTDQPTRHTFG
MSMEG_2252|M.smegmatis_MC2_155 SIEIMDQRGLLDRFLEVGRRHP-----VGGYFAGITKAPPRTLDTAHPYV
TH_1880|M.thermoresistible__bu SIEMLDQRGLLDRFLAHGQRHP-----VGGFFAAIAK-PPAELDTAHGYV
Mflv_5009|M.gilvum_PYR-GCK GSEVLDRMGALGTLPQESLRALRITNYLGDRPLATLEFGDPGMRTAAPP-
Mb1780|M.bovis_AF2122/97 TMRLLDELGLWERFAALPYSEVR-TATLHSNGRAVTYIDFERLHQPYPYV
Rv1751|M.tuberculosis_H37Rv TMRLLDELGLWERFAALPYSEVR-TATLHSNGRAVTYIDFERLHQPYPYV
MUL_3663|M.ulcerans_Agy99 TLRLLDELGLGSQFAQIPHRLID-TISMKVQGQPVD-LDLKHLPGAHQHI
.::*. * : :
MMAR_4271|M.marinum_M LALWQNRIERILAEWVAELR-VRIYRGCEVTDITQ-------DDNGVDLT
MAV_2736|M.avium_104 LALWQNHIERILADWVGELG-VPIYRGHRIATCTQ-------DDTGVDVV
Mvan_2422|M.vanbaalenii_PYR-1 LALWQKHIERILAEWVDELA-VPVYRGREVVGVRQ-------DGAGVDIE
MAB_3483|M.abscessus_ATCC_1997 LALLQYRIERTLSEWVGEHG-VPIYRGREVTGFTS-------SDDAVRVE
MSMEG_2252|M.smegmatis_MC2_155 LGIPQPVTDRILVEHAVASG-AVLRRGCEAVGLAQ-------DDDGVSVT
TH_1880|M.thermoresistible__bu LGIRQPVTDRLLAEWAAERG-AVIRRGCAVVGLDQ-------DAAGITVE
Mflv_5009|M.gilvum_PYR-GCK MVVSQAKVEAALRARLADLD-VEPEWGRKVIDVRE-------DADGVVAD
Mb1780|M.bovis_AF2122/97 AMVPQWDLLNLLAEAAQAEPSFTLRMKTEVTGLLR----EGGKVTGVRYQ
Rv1751|M.tuberculosis_H37Rv AMVPQWDLLNLLAEAAQAEPSFTLRMKTEVTGLLR----EGGKVTGVRYQ
MUL_3663|M.ulcerans_Agy99 ALVPQWDFLELLAAAAEAEPTFRLLRSTEVTGVLWGDGPDPGRVVSATYR
: * * .
MMAR_4271|M.marinum_M LSDG--PPLRADYVVGCDGGRSLVRRAVGIDFPGWDPTTSSLIAEVEVAV
MAV_2736|M.avium_104 ASDG--RTLRGQYLVGCDGGRSVIRKAAGIDFPGWEATTSYLLAEAEMDW
Mvan_2422|M.vanbaalenii_PYR-1 LSDG--RVLRAGYLVGCDGGRSLIRKSAGIEFPGWEPTTSYLLAEVEIAP
MAB_3483|M.abscessus_ATCC_1997 LSDG--STLRAQYLVGCDGGRSLTRKIAGIDFPGWDPSTSCLVADVQMT-
MSMEG_2252|M.smegmatis_MC2_155 LADG--TQVRARYVVGCDGGRSTVRKLMGVGFPGEPSRVDTLIGEMQVGV
TH_1880|M.thermoresistible__bu LADG--SVQRARYLVGCDGGRSTVRKLAGIGFPGEPARSETLLGEMSLSA
Mflv_5009|M.gilvum_PYR-GCK LADG--ARVRGQWLVGCDGTTSVVRRQTGIEFPGVRLSERFLLADIHLDW
Mb1780|M.bovis_AF2122/97 GAEG-PGELRAELTVACDGRWSIARHEAGLKAREFPVNFDVWWFKLPREG
Rv1751|M.tuberculosis_H37Rv GAEG-PGELRAELTVACDGRWSIARHEAGLKAREFPVNFDVWWFKLPREG
MUL_3663|M.ulcerans_Agy99 AQSGETKHMHADLTVACDGRGSTVRSALGMATRSFGAPMDVWWFRLPRRP
.* :. *.*** * * *:
MMAR_4271|M.marinum_M G-SESGPNLGEVRHDALGVHGFTR---LPDGGPLRVVVTERQLDAGGEPS
MAV_2736|M.avium_104 R-TGQPPQWG-IHHDSLGVHALSE---TEPAGGVRVLVTERQLRSAAEPN
Mvan_2422|M.vanbaalenii_PYR-1 E-DGREPEWG-IRRDALGVHALSR---SDD-GPVRIMVTEQDLRRSGVPD
MAB_3483|M.abscessus_ATCC_1997 ----EEPEWGIHRQGAL--HSIFK---FEEDGPVRVLVTEAQLGRREEPT
MSMEG_2252|M.smegmatis_MC2_155 TPEQVTAVVAEVRKMQLRFGAGPL---GDGLYRVMVPAAEVVEDRSATPT
TH_1880|M.thermoresistible__bu DPATVAAVVAEVRKTQLRFGAMPL---GDGVYRVIVPAEGVAGDR-AEPT
Mflv_5009|M.gilvum_PYR-GCK AVDRAGTTGWIHPDGVVGAMPMPSTDGRDDLWRLFVYDPSLDRKPTDSEI
Mb1780|M.bovis_AF2122/97 D---AEFSFLPRFSPGKGLGVIPREGYFQIAYLGPKGTDAQLRERGIEEF
Rv1751|M.tuberculosis_H37Rv D---AEFSFLPRFSPGKGLGVIPREGYFQIAYLGPKGTDAQLRERGIEEF
MUL_3663|M.ulcerans_Agy99 D---DPTGLAGIFNSGHGGIAIDRGDYYQIAYIIPKGSDAQLRAQGIEAL
MMAR_4271|M.marinum_M LGDLSAALTAVYGTDYGIHSPTWISRFTDLTRQAATYRQGRVLIAGDAAH
MAV_2736|M.avium_104 LDDLRQALIAVYRTDYGIHSPRWISRFTDAARQAARYRDRRVLLAGDAAH
Mvan_2422|M.vanbaalenii_PYR-1 LAELREALVTVYGTDFGIHSPTSISRFTDTARQAVAYRSGRILLVGDAAH
MAB_3483|M.abscessus_ATCC_1997 LRDLSEALLSARGTDYGIHSPTWISRFTDVTRQAATYRKGRVLLAGDAAH
MSMEG_2252|M.smegmatis_MC2_155 LDEFRQQLRATAGTDLGVHSPRWLSRFGDGTRLADRYRTGRVLLAGDAAH
TH_1880|M.thermoresistible__bu LDEFRRRLKAFAGTDFGAHTPRWLSRFGDATRLAERYRAGRVFLAGDAAH
Mflv_5009|M.gilvum_PYR-GCK LDRISELVPYRTGRRVKIGDAEWLSMFTVHRRLADTYRRGRVLIAGDAAH
Mb1780|M.bovis_AF2122/97 RRDVSELLPEATASVAALASTDEVKHLNVKVNRLRRWHIDGLLCIGDAAH
Rv1751|M.tuberculosis_H37Rv RRDVSELLPEATASVAALASMDEVKHLNVKVNRLRRWHIDGLLCIGDAAH
MUL_3663|M.ulcerans_Agy99 HRALADLMPWLADRVDALKSFDDVKLLDVQLNRLRRWYCDGALCIGDAAH
. : :. : . : : *****
MMAR_4271|M.marinum_M VHDPVGGQGLNTGVQDAVNLGWKLAQVVNRR--SPESLLDTYHAERHPVA
MAV_2736|M.avium_104 IHHPVGGQGLNTGVQDAVNLGWKLALVVGGA--SPPALLDTYHAERHPVG
Mvan_2422|M.vanbaalenii_PYR-1 VHHPVGGQGLNTGMQDAVNLGWKLAQVVQGW--SPDTLLDTYHDERQPVG
MAB_3483|M.abscessus_ATCC_1997 VHFPIGGQGLNTGMQDAVNLGWKLAQVVNGT--SPENLLDTYHAERHPVA
MSMEG_2252|M.smegmatis_MC2_155 VHPPVGGQGLNLGVQDAFNLGWKLAASINGW--APDGLLDSYEAERRPVA
TH_1880|M.thermoresistible__bu VHPPAGGQGLNLGIQDAFNLGWKLAAAVGGW--APDGLLDTYHAERHPVA
Mflv_5009|M.gilvum_PYR-GCK VHAPFGGQGMLTGIGDAENVGFKLALVVRGL--AADDLLDTYEAERRPLA
Mb1780|M.bovis_AF2122/97 AMSPVAGVGINLAVQDAVAAATILAEPLREHRVSSRHLAAVRRRRAFPTA
Rv1751|M.tuberculosis_H37Rv AMSPVAGVGINLAVQDAVAAATILAEPLREHRVSSRHLAAVRRRRAFPTA
MUL_3663|M.ulcerans_Agy99 AMSPVGGVGINLALADAVATARMLAPSLRVGRVSTGQLARVQARRWAPTA
* .* *: .: ** . ** : :. * . * .
MMAR_4271|M.marinum_M ARVLRTTMAQVALRRL-DYRKKALSDTIS-ELLMMDEPRKRFAAMMSGLD
MAV_2736|M.avium_104 ERVLRNAIAQMALLRP-DERTRALRDLVT-ELLGMDEPRKRFAAMISGLD
Mvan_2422|M.vanbaalenii_PYR-1 ARVLRNAVAQMALLRP-DDRTTVLHGVVS-DMLDMDEPRRRFAAMMSGLD
MAB_3483|M.abscessus_ATCC_1997 ARVLHNTMAQTALIRP-GERVDALRDIMS-DLLTLDMPRRRIAAMMSGLD
MSMEG_2252|M.smegmatis_MC2_155 AAVLDNTRAQMELLSP-EPGPQAVRRLLA-QLMAFEEVNRYLTEMITATG
TH_1880|M.thermoresistible__bu ADVLNNTRAQMELMSP-EPGPRAVRWLLA-ELMDFEEVNRYLTEKITAIG
Mflv_5009|M.gilvum_PYR-GCK TQVLRGTSAITRVNVAGNPIVRFLRDRVAPRVFGLPAVQRWTTYTASQLW
Mb1780|M.bovis_AF2122/97 VTQAVQRVLHRRLLGPLLQGRDPTPPAALLGLVERLPWLSAVPAYFVGVG
Rv1751|M.tuberculosis_H37Rv VTQAVQRVLHRRLLGPLLQGRDPTPPAALLGLVERLPWLSAVPAYFVGVG
MUL_3663|M.ulcerans_Agy99 FLQGIQRAIHAAIVAVAVTGGPPQPPPVVR-LVSRSVPVRRIMGYLVAIG
: :.
MMAR_4271|M.marinum_M IRYH-----LGGGHPLLGRRMPDLDLTTADGPRRVFTHLHAARPVLLNLA
MAV_2736|M.avium_104 VRYD-----LGPGHPLLGRRMPDLDLVTADGASRVFALLRNARPVLLNFA
Mvan_2422|M.vanbaalenii_PYR-1 IHYD-----LGDGHPLLGLRMPDLDLETAHGPTRVSALLRQARPVLLDLG
MAB_3483|M.abscessus_ATCC_1997 IHYD-----LGPGHPLLGRRMPDLEVRTAQGPRCVFTLLREGRGVLLNLG
MSMEG_2252|M.smegmatis_MC2_155 IRYD-----FGAEDDLVGRRLRDIRLRRG----RLYELTRTGRGLLLDQT
TH_1880|M.thermoresistible__bu VRYHT----MGDGHGLLGRRMRDIPLTGG----RLYARMHAGRGVLLDRT
Mflv_5009|M.gilvum_PYR-GCK VSYRNGPLGGRGSKPRPGDRIDDAPCSRPDGTATRLHGELGGRWALLLPR
Mb1780|M.bovis_AF2122/97 VRPEH--------APAFARRGPGNRKGP----------------------
Rv1751|M.tuberculosis_H37Rv VRPEH--------APAFARRGPGNRKGP----------------------
MUL_3663|M.ulcerans_Agy99 PLPEH--------APKYPRRAR----------------------------
*
MMAR_4271|M.marinum_M EPGRFDIAGWRHRVLLIDAK---YVGVWELPVLGAIAAPAAALIRPDGYV
MAV_2736|M.avium_104 GAGRRSVAGWADRVRVVDAK---CVADWELPVLGRVSAPPAVLIRPDGHV
Mvan_2422|M.vanbaalenii_PYR-1 APDAVDLTGWEARVRMVRAN---AATVWELPVIGEVPAPAAVLIRPDGHV
MAB_3483|M.abscessus_ATCC_1997 TP-ITGASGWSHRVDVVEAEYLDAEGTFELPVIGMVEAPHAVLIRPDGYV
MSMEG_2252|M.smegmatis_MC2_155 GQ--LSAAGWSDRVDHVVDV-------------SDELDVPAVLLRPDGHI
TH_1880|M.thermoresistible__bu GT--LSVSGWADRVDHIVDP-------------GAALDVPAVLLRPDGHV
Mflv_5009|M.gilvum_PYR-GCK GVPAVGAAAVRGPAGYLADH----------VVTLHHDRDDVMLVRPDAHL
Mb1780|M.bovis_AF2122/97 --------------------------------------------------
Rv1751|M.tuberculosis_H37Rv --------------------------------------------------
MUL_3663|M.ulcerans_Agy99 --------------------------------------------------
MMAR_4271|M.marinum_M AWVGDD-LTDRGLFDALTAWFGRPAVPSPRLRASAP
MAV_2736|M.avium_104 AWAGD--PNDPGLHDALTTWCGPPAAGG--------
Mvan_2422|M.vanbaalenii_PYR-1 AWVAG--DSAAAPAEAAARWFGAPTGRR--------
MAB_3483|M.abscessus_ATCC_1997 AWVGN--GTTAGLNGALQRWFGPPRYSGGGS-----
MSMEG_2252|M.smegmatis_MC2_155 AWVGDE---QGDLEGCLVRWFGVPA-----------
TH_1880|M.thermoresistible__bu AWVGADHPGQQDLTARLSRWFGAPA-----------
Mflv_5009|M.gilvum_PYR-GCK AWRGAH-----DDPTGLDRWLATALHPGGTQ-----
Mb1780|M.bovis_AF2122/97 ------------------------------------
Rv1751|M.tuberculosis_H37Rv ------------------------------------
MUL_3663|M.ulcerans_Agy99 ------------------------------------