For questions or suggestions e-mail us at: ioerger@cs.tamu.edu
MSIVGKNDIQQLVAAGQEGLAKGRLPAGLVANAELHKLEAQRVFGRCWQFLAHETEIPQAGDYVVRYLGG GSIIVVRGEDGEVRAMANSCRHRGTMLCRTEMGNTSHFRCPYHGWTYRNTGTLAGVPAQKEVYGVEMDKN EWSLTQVPRLENYRGMIFGCLDEKAEPLVDYLGDMAWYLDLITQKSKGGLEVRGEPQRWIIDSNWKLGAD NFVGDAYHTLMTHRSAVELGLAPPDPKFASEPAHISLSNGHGLGVLGVPPGQPMPPFMNYPPEVVDGLAA AYGDQDRADMLQRSAFIHGTVFPNLSFLNVLIGRDKKSMPVPMLTFRLWRPLSHDTMEVWSWFLVEKDAD EEFKQQSYETYVRTFGISGVFEQDDAETWRSITAGTQGILAGSQTLNFEMGMGVLTSDDTWKGPGRPLSS GYAERNQREFWGRLLELLTDSGDDASETEPKPQLLAQSRTNTDEVA
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. vanbaalenii PYR-1 | Mvan_0546 | - | - | 100% (466) | ring hydroxylating dioxygenase, alpha subunit |
| M. vanbaalenii PYR-1 | Mvan_0525 | - | e-165 | 63.72% (419) | ring hydroxylating dioxygenase, alpha subunit |
| M. vanbaalenii PYR-1 | Mvan_0488 | - | e-149 | 57.35% (422) | ring hydroxylating dioxygenase, alpha subunit |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb3186c | - | 1e-22 | 31.40% (207) | dioxygenase |
| M. gilvum PYR-GCK | Mflv_0537 | - | 0.0 | 99.14% (466) | ring hydroxylating dioxygenase, alpha subunit |
| M. tuberculosis H37Rv | Rv3161c | - | 9e-23 | 31.40% (207) | dioxygenase |
| M. leprae Br4923 | - | - | - | - | - |
| M. abscessus ATCC 19977 | MAB_4370c | - | 2e-66 | 34.27% (426) | putative large terminal subunit of phenylpropionate dioxygenase |
| M. marinum M | MMAR_0212 | - | 6e-24 | 27.47% (324) | oxidoreductase |
| M. avium 104 | MAV_3509 | - | 9e-89 | 41.22% (427) | phthalate dioxygenase large subunit |
| M. smegmatis MC2 155 | MSMEG_1908 | - | 1e-41 | 28.57% (420) | benzoate 1,2-dioxygenase alpha subunit |
| M. thermoresistible (build 8) | TH_2976 | - | 9e-36 | 26.65% (424) | PUTATIVE dioxygenase large subunit |
| M. ulcerans Agy99 | MUL_3820 | - | 3e-17 | 32.96% (179) | ring-hydroxylating dioxygenase, large terminal subunit |
CLUSTAL 2.0.9 multiple sequence alignment
Mb3186c|M.bovis_AF2122/97 MLSTDNRAELGDILTDIGDYLDDNPPAL--------SLPPAAYTSSELWQ
Rv3161c|M.tuberculosis_H37Rv MLSTDNRAELGDILTDIGDYLDDNPPAL--------SLPPAAYTSSELWQ
Mvan_0546|M.vanbaalenii_PYR-1 -MSIVGKNDIQQLVAAGQEGLAKG------------RLPAGLVANAELHK
Mflv_0537|M.gilvum_PYR-GCK -MSTVGKNDIQQLVAAGREGLAKG------------RLPAGLVANAELHK
MAV_3509|M.avium_104 ---MAGPD---GGLLGVLENVRRG------------MIPAHIYNDPELFA
MAB_4370c|M.abscessus_ATCC_199 MASLPRPVAVAQGALCRPQALVRDIDVSGLVDSHGGLLDRVIFTDGELYR
MSMEG_1908|M.smegmatis_MC2_155 MTTHLSPDHLENVLADAVIEDPAAG---------VYRANRRIFTDDEIFE
TH_2976|M.thermoresistible__bu ---LIRPD----------------------------RVHGSLYTDPAIFA
MMAR_0212|M.marinum_M -MSTAAGNPARTRDEDAIGVPPASP----------TLVPAQRYHSPAFAQ
MUL_3820|M.ulcerans_Agy99 -MDRDQLIDLTRRALKLARDKTTDLAP------HQHAVDAREYTSLDRHE
.
Mb3186c|M.bovis_AF2122/97 LERERIFNRSWMLVAHVDQLAKTGDYVTVSVAGEPVMVVRDVDGQLHALS
Rv3161c|M.tuberculosis_H37Rv LERERIFNRSWMLVAHVDQVAKTGDYVTVSVAGEPVMVVRDVDGQLHALS
Mvan_0546|M.vanbaalenii_PYR-1 LEAQRVFGRCWQFLAHETEIPQAGDYVVRYLGGGSIIVVRGEDGEVRAMA
Mflv_0537|M.gilvum_PYR-GCK LEAQRVFGRCWQFLAHETEIPQAGDYVVRYLGGGSIIVVRGEDGEVRAMA
MAV_3509|M.avium_104 LEKRRLFARAWTFVGHESEIPHDGDYMVRRVLDDSFIITRGSDGRVRALF
MAB_4370c|M.abscessus_ATCC_199 QELRRVFAPSWLFLAHADQFHRPGDFFTTYMGEDPVIVSMDKNRRIRAFL
MSMEG_1908|M.smegmatis_MC2_155 LEMKHIFEGNWVYLAHESQVPNPGDYFTTYIGRQPVVITRDRDGDLHCLI
TH_2976|M.thermoresistible__bu EELRKIWYRTWVFVGHESEVSQPNDYVRKKLGLQDVIMTRDRDGEIHLLL
MMAR_0212|M.marinum_M LEIERMWPKVWQLACMLDHVAAPGDYFEYRCGPYSVLIVHDCDGVLRAFQ
MUL_3820|M.ulcerans_Agy99 RDRAMVLD-SPQLVGYSSELPGPDTYRTKTIMGRSILLTRAADRAVRAFD
: : .. . : .:: : :: :
Mb3186c|M.bovis_AF2122/97 PICRHRLMLMVEPGAGRIDTLTCQYHLWRYGLDGRLRGAPH-MAANLD--
Rv3161c|M.tuberculosis_H37Rv PICRHRLMLMVEPGAGRIDTLTCQYHLWRYGLDGRLRGAPH-MAANLD--
Mvan_0546|M.vanbaalenii_PYR-1 NSCRHRGTMLCRTEMGNTSHFRCPYHGWTYRNTGTLAGVPA-QKEVYG-V
Mflv_0537|M.gilvum_PYR-GCK NSCRHRGTMLCRTEMGNTSHFRCPYHGWTYRNTGTLAGVPA-QKEVYG-V
MAV_3509|M.avium_104 NMCLHRGMQVCRAELGNASNFRCPYHGWTYRNDGRLTGLPF-HREAYGGD
MAB_4370c|M.abscessus_ATCC_199 NSCRHRGARVCRADYGVTRGFTCTYHGWAYDLEGKLISVPN-EEGYPD--
MSMEG_1908|M.smegmatis_MC2_155 NACAHRGAMICRRKTDNRTTLTCPFHGWTFRNDGTLLKVKDPDGAGYPDT
TH_2976|M.thermoresistible__bu NRCSHRGNQVCADAKGNSSTFRCPYHGWTFRNTGELVGFPFFKGYGTR--
MMAR_0212|M.marinum_M NVCRHRGNSLCAGSGSGLRELKCGYHGWTWDLTGELRRVPN--RKGFG--
MUL_3820|M.ulcerans_Agy99 NVCLHRQSRVATG-CGTALRFTCPYHGWTYDNGGRLVGLPG--REGFP--
* ** : . : * :* * : * *
Mb3186c|M.bovis_AF2122/97 --FNRRECRLPQFAVATWNGLVWINLDADA-EPIAAHLD----LTDDEFA
Rv3161c|M.tuberculosis_H37Rv --FNRRECRLPQFAVATWNGLVWINLDADA-EPIAAHLD----LTDDEFA
Mvan_0546|M.vanbaalenii_PYR-1 EMDKNEWSLTQVPRLENYRGMIFGCLDEKA-EPLVDYLGDMAWYLDLITQ
Mflv_0537|M.gilvum_PYR-GCK EMDKNEWSLTQVPRLENYRGMIFGCLDEKA-EPLVDYLGDMAWYLDLITQ
MAV_3509|M.avium_104 DGFVKDQTLLPAPNFAGYNGLLFISLDPAA-PPLQDFLGDFRFYLDYYTR
MAB_4370c|M.abscessus_ATCC_199 SFSTDDWGLVEVPHVDSYHGLIFGTWNPAP-VGLRESLGDMAWYMDAMLD
MSMEG_1908|M.smegmatis_MC2_155 FNSNGSHNMTNVARFEGYRGFLFGSLNPDV-LPLTEHLGDTTTVIDMLVD
TH_2976|M.thermoresistible__bu ---KLDLPLGRVPRVDSYGGFVFGSFATEG-PTLREHLGAAAGEIDRLLR
MMAR_0212|M.marinum_M SLHMPDFPLLPVR-VETWEGLVFVSLDVTA-MPLMDYLDPIPEDIAWCGL
MUL_3820|M.ulcerans_Agy99 -GIAVGSDGLTELPATEFAGFLWVSLHPAARLDVPAHLGPLAGELQSWGI
: *::: : *.
Mb3186c|M.bovis_AF2122/97 GYRLG-EMVQVESWSHEWRANWKVAAEN-GHENYHVLGLHRQTLEP----
Rv3161c|M.tuberculosis_H37Rv GYRLG-EMVQVESWSHEWRANWKVAAEN-GHENYHVLGLHRQTLEP----
Mvan_0546|M.vanbaalenii_PYR-1 KSKGG-LEVRGEPQRWIIDSNWKLGADNFVGDAYHTLMTHRSAVELGLAP
Mflv_0537|M.gilvum_PYR-GCK KSKGG-LEVRGEPQRWIIDSNWKLGADNFVGDAYHTLMTHRSAVELGLAP
MAV_3509|M.avium_104 QSAGG-VELRG-PQRWRIRANWKIGAENFAGDMYHTPHTHASIVDIGLFR
MAB_4370c|M.abscessus_ATCC_199 HDSEG-TVVVGGVHKWVLDGNWKLAAEQFATDWYHVNMSHASALMVMAP-
MSMEG_1908|M.smegmatis_MC2_155 QSPDG-LEVLRGASTYTYDGNWKVQAEN-GADGYHVTATHWNYAATTSRR
TH_2976|M.thermoresistible__bu LSPEGKVALDAGWLRHRTRANWKLLVEN-ETDGYHPQFVHGGIFGVTGST
MMAR_0212|M.marinum_M SDFRC-----YATLTVEVDANWKTIADG-YSETYHIQTLHPELLRCVDDI
MUL_3820|M.ulcerans_Agy99 GRWSP-----LGEKVLGSPIDWKLATDT-FAENYHFATVHRRTFATIAR-
:** .: : ** *
Mb3186c|M.bovis_AF2122/97 -----------------------FVPGGGDLDVRQYSRWALRLRVPFTVP
Rv3161c|M.tuberculosis_H37Rv -----------------------FVPGGGDLDVRQYSRWALRLRVPFTVP
Mvan_0546|M.vanbaalenii_PYR-1 PDPKFASEPAHISLSNGHGLGVLGVPPG--QPMPPFMNYPPEVVDGLAAA
Mflv_0537|M.gilvum_PYR-GCK PDPKFASEPAHISLSNGHGLGVLGVTPG--QPMPPFMNYPPEVVDGLAAA
MAV_3509|M.avium_104 EPKAQKRKDGATYWAHRGGGTTYKLPPGGFEERMRYVGYPDEMIGRIKQV
MAB_4370c|M.abscessus_ATCC_199 ----QGKAPKDEIVHRTGRQYVDPNGHGAGFPVHPKNRFDARVVHQWTDY
MSMEG_1908|M.smegmatis_MC2_155 TCGESKNDTKALDAGSWGKSGGGYWSFPHGHLCLWTWAANPQDRPLWDRL
TH_2976|M.thermoresistible__bu IGPLYSDSSTAVTRDLGGGHSENDLRPEFRKFAEPLRWFGTTESRVPDYV
MMAR_0212|M.marinum_M HAPQQIWRHAGKSDQPYGVPSPRFEGTLSDQEVWDAYVYTQGALMGAAEG
MUL_3820|M.ulcerans_Agy99 ----------------------------SNCTVFDSYGPHHRLIFPLNTI
Mb3186c|M.bovis_AF2122/97 VEAKSLQ--LNEVQKSN-LVVLWTFPNSALAIAG--------------ER
Rv3161c|M.tuberculosis_H37Rv VEAKSLQ--LNEVQKSN-LVVLWTFPNSALAIAG--------------ER
Mvan_0546|M.vanbaalenii_PYR-1 YGDQDRA--DMLQRSAF-IHG-TVFPNLSFLN----VLIGRDKKSMPVPM
Mflv_0537|M.gilvum_PYR-GCK YGDQDRA--DMLQRSAF-IHG-TVFPNLSFLN----VLIGRDKKSMPVPM
MAV_3509|M.avium_104 WTPRQQR--VVGEDGFM-VSAATCFPNLSFVHNWPKVRDGRDDE--TLPF
MAB_4370c|M.abscessus_ATCC_199 GALRERLGEARVQGPLT-NGHATVFPNLSYLPVN----------------
MSMEG_1908|M.smegmatis_MC2_155 DELKARFGDAKGEFMVKGSRNLCLYPNVYVMDQFS---------------
TH_2976|M.thermoresistible__bu AAMRARYGAEADQILIEGSPHVMIFPNLFIAE------------------
MMAR_0212|M.marinum_M TPLPADERRPGQSVQDLIAARTRAFAATRGVDLEWADTDR-------ITR
MUL_3820|M.ulcerans_Agy99 LELENVP-EGQWDPFHHMVVIYALFPNVVLSVTIAN--------------
:.
Mb3186c|M.bovis_AF2122/97 VVWFGFIP-----QSIDRVQVLGGVLTTPELAADAAATAQTSQFVMA---
Rv3161c|M.tuberculosis_H37Rv VVWFGFIP-----QSIDRVQVLGGVLTTPELAADAAATAQTSQFVMA---
Mvan_0546|M.vanbaalenii_PYR-1 LTFRLWRP-----LSHDTMEVWSWFLVEKDADEEFKQQSYETYVRTFGIS
Mflv_0537|M.gilvum_PYR-GCK LTFRLWRP-----LSHDTMEVWSWFLVEKDADEEFKQQSYETYVRTFGIS
MAV_3509|M.avium_104 ISIRLWQP-----ISENETEVCSWFAVDSAAPAHYKQDSYKAYLMCFGST
MAB_4370c|M.abscessus_ATCC_199 GSVRIWHP-----KGPDKMEVWAWTIVDRSMPEEVRDAQRLYNLRTFGPT
MSMEG_1908|M.smegmatis_MC2_155 TQIRHFRP-----IAPDKTEVTIYCIAPKGESAAARAHRIRQYEDFFNAS
TH_2976|M.thermoresistible__bu IQVFNIQP-----VAVNECVQYSTAVQLVGAPELNRR-MVSQCIGSVGPA
MMAR_0212|M.marinum_M LHQYNVFPNMTFLANADHLTIMCSRPQPEHFADPDKGEMVMFLMTRMPPD
MUL_3820|M.ulcerans_Agy99 GELFRIYP-------GDRPGSSITVHQNSTPLDLSDESVATGAQAVFDYA
* :
Mb3186c|M.bovis_AF2122/97 -MINDEDRLGLEAVQVGAGSRFAERGHLSSKEWPG---------------
Rv3161c|M.tuberculosis_H37Rv -MINDEDRLGLEAVQVGAGSRFAERGHLSSKEWPG---------------
Mvan_0546|M.vanbaalenii_PYR-1 GVFEQDDAETWRSITAGTQGILAGSQTLNFEMGMG-------VLTSDDTW
Mflv_0537|M.gilvum_PYR-GCK GVFEQDDAETWRSITAGTQGILAGSQTLNFEMGMG-------VLTSDDTW
MAV_3509|M.avium_104 GMFEQDDVENWVSLTTTAGGSMARRLLLNSRMGLYDDGRPVVEALAPSAF
MAB_4370c|M.abscessus_ATCC_199 GIFEQDDGENWSEVQSISRGFVTNSVALNYQMGLD----------SERED
MSMEG_1908|M.smegmatis_MC2_155 GMATPDDLEEFRSCQLTFRAQAAPWNDMSRGAQHWLS-----GPDPVAES
TH_2976|M.thermoresistible__bu GMLLADDTEMYERNQQGLEALTPEWLDIRRGLNREH-------------V
MMAR_0212|M.marinum_M APHNKPTDVRMAADQAEPGIVLTQDIRVLPGVQRG----------LHQPG
MUL_3820|M.ulcerans_Agy99 HATVRDEDYRLAETLQTNLESGARQRLVFGRNEPG---------------
. :
Mb3186c|M.bovis_AF2122/97 ---------------MLAFYRNLAMALVGDHPGAS---------------
Rv3161c|M.tuberculosis_H37Rv ---------------MLAFYRNLAMALVGDHPGAS---------------
Mvan_0546|M.vanbaalenii_PYR-1 KGPGRPLSSGYAERNQREFWGRLLELLTDSGDDASETEPKPQLLAQSRTN
Mflv_0537|M.gilvum_PYR-GCK KGPGRPLSSGYAERNQREFWGRLLELLTDSGDDASETEPKPQLLAQSRTN
MAV_3509|M.avium_104 HGPGR-AQVGYNEHNQRALLAMWADYLQEG--DACENR------------
MAB_4370c|M.abscessus_ATCC_199 GRHPGLTSELYSDAAGRSFYARWRDLMNTKAWHEEHV-------------
MSMEG_1908|M.smegmatis_MC2_155 LGMHGIVSSGLRNEDEGLYPVQHGYWLETLRRAVTSNPTEH---------
TH_2976|M.thermoresistible__bu DDHGFTVGGPTDETGMRAFWSEYRRLMEAE--------------------
MMAR_0212|M.marinum_M FTHLVLSGEERRIINMHRNLERYLDLPEPERMSGGGLRPGPGAPRV----
MUL_3820|M.ulcerans_Agy99 ---------------LQHRHITWAQALETPG-------------------
Mb3186c|M.bovis_AF2122/97 -----
Rv3161c|M.tuberculosis_H37Rv -----
Mvan_0546|M.vanbaalenii_PYR-1 TDEVA
Mflv_0537|M.gilvum_PYR-GCK ADEVA
MAV_3509|M.avium_104 -----
MAB_4370c|M.abscessus_ATCC_199 -----
MSMEG_1908|M.smegmatis_MC2_155 -----
TH_2976|M.thermoresistible__bu -----
MMAR_0212|M.marinum_M -----
MUL_3820|M.ulcerans_Agy99 -----