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MTALMATLEGHYYTSAEVFAAEQQGIFENMWFCAVRTSDLEKPGKFTKVQVGRESVLLVRGRDGLLRAFL NVCRHRGAQLCSESEGEVRRTLRCPYHSWTYALDGKLVAAPNIGTLTDDAGSPIDRYRYGLIPVALTEWL GYAWVCLSDTPPPFEDVVAEATRTLGDADAINRYGIGGLDVGHRVVYEVAANWKLIVENFMECYHCSSIH PELVDVLPEFAKGQAAQANIGLGAEFGSEVAGFTVDGGAGFERLPGIADDQDRRYYAITVKPTVFINLVP DHVIFHRMYPVAADRTVVECDWLYTREVLGSGRDVSRSVELFHRVNMQDFDACERTQPAMSSRAYRSGGV LVPAEHHLAEFHRWVVSRLATPV
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. gilvum PYR-GCK | Mflv_3082 | - | - | 100% (373) | Rieske (2Fe-2S) domain-containing protein |
| M. gilvum PYR-GCK | Mflv_2328 | - | 3e-30 | 31.23% (253) | Rieske (2Fe-2S) domain-containing protein |
| M. gilvum PYR-GCK | Mflv_0632 | - | 2e-24 | 29.38% (211) | ring hydroxylating dioxygenase, alpha subunit |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb3186c | - | 1e-22 | 31.53% (203) | dioxygenase |
| M. tuberculosis H37Rv | Rv3161c | - | 3e-22 | 31.03% (203) | dioxygenase |
| M. leprae Br4923 | - | - | - | - | - |
| M. abscessus ATCC 19977 | MAB_4370c | - | 6e-20 | 31.39% (223) | putative large terminal subunit of phenylpropionate dioxygenase |
| M. marinum M | MMAR_4422 | - | 1e-164 | 73.12% (372) | dioxygenase |
| M. avium 104 | MAV_3509 | - | 2e-24 | 29.86% (221) | phthalate dioxygenase large subunit |
| M. smegmatis MC2 155 | MSMEG_0657 | - | 6e-56 | 37.01% (354) | Rieske (2Fe-2S) domain-containing protein |
| M. thermoresistible (build 8) | TH_3820 | - | 5e-23 | 29.17% (240) | PUTATIVE Rieske [2Fe-2S] domain protein |
| M. ulcerans Agy99 | MUL_3820 | - | 1e-15 | 34.66% (176) | ring-hydroxylating dioxygenase, large terminal subunit |
| M. vanbaalenii PYR-1 | Mvan_4319 | - | 3e-31 | 32.41% (253) | Rieske (2Fe-2S) domain-containing protein |
CLUSTAL 2.0.9 multiple sequence alignment
Mflv_3082|M.gilvum_PYR-GCK --------------------------------MTAL---MATLEGHYYTS
MMAR_4422|M.marinum_M --------------------------MTAVRQPSAL---LPTLGGAYYTS
MSMEG_0657|M.smegmatis_MC2_155 MDHFPAADYPDCMFKQIGPAPVPADDLAAALAPFGH---SRMLPREAYVD
Mb3186c|M.bovis_AF2122/97 ----------MLSTDNRAELGDILTDIGDYLDDNPP---ALSLPPAAYTS
Rv3161c|M.tuberculosis_H37Rv ----------MLSTDNRAELGDILTDIGDYLDDNPP---ALSLPPAAYTS
TH_3820|M.thermoresistible__bu ----------------VAHFPKPAVGSWTENWPELG---TAPVDYTDSID
Mvan_4319|M.vanbaalenii_PYR-1 -----------MPATRPGEWLDPASGLGLGLDDVQPGTFNMTISTDRYTC
MAV_3509|M.avium_104 -------------------MAGPDGGLLGVLENVRR----GMIPAHIYND
MAB_4370c|M.abscessus_ATCC_199 -----MASLPRPVAVAQGALCRPQALVRDIDVSGLVDSHGGLLDRVIFTD
MUL_3820|M.ulcerans_Agy99 -----------MDRDQLIDLTRRALKLARDKTTDLAP-HQHAVDAREYTS
:
Mflv_3082|M.gilvum_PYR-GCK AEVFAAEQQGIFENMWFCAVRTSDLEKPGKFTKVQVG----RESVLLVRG
MMAR_4422|M.marinum_M AAVFAAEQQHVFESMWFCVVRAADLNEPGQFKAVQVG----RESVLLVRG
MSMEG_0657|M.smegmatis_MC2_155 PAVFAWEQQAIFS-GWTCVGHAADLETPGAQRAVGSG----ANGVLLVRG
Mb3186c|M.bovis_AF2122/97 SELWQLERERIFNRSWMLVAHVDQLAKTGDYVTVSVA----GEPVMVVRD
Rv3161c|M.tuberculosis_H37Rv SELWQLERERIFNRSWMLVAHVDQVAKTGDYVTVSVA----GEPVMVVRD
TH_3820|M.thermoresistible__bu PEQWKLEQQAIFRRLWLHVGRVERLPKAGSYFTREMPSVGAGTSIIVNRD
Mvan_4319|M.vanbaalenii_PYR-1 PDYAARERGSIWMRTWQIAGRVEDLPKPGDWKKYEIL----DQSFIIVRG
MAV_3509|M.avium_104 PELFALEKRRLFARAWTFVGHESEIPHDGDYMVRRVL----DDSFIITRG
MAB_4370c|M.abscessus_ATCC_199 GELYRQELRRVFAPSWLFLAHADQFHRPGDFFTTYMG----EDPVIVSMD
MUL_3820|M.ulcerans_Agy99 LDRHERDR-AMVLDSPQLVGYSSELPGPDTYRTKTIMG----RSILLTRA
: : . . .::
Mflv_3082|M.gilvum_PYR-GCK RDGLLRAFLNVCRHRGAQLCS-----ESEGEVRRTLRCPYHSWTYALDGK
MMAR_4422|M.marinum_M RDRRLRAFLNICRHRGALLCT-----EPEGQVRRNLRCPYHSWTYGLDGT
MSMEG_0657|M.smegmatis_MC2_155 EDNRVRAFANACRHRGHELLS-----CGASAKRRSIVCPYHAWSYRLDGS
Mb3186c|M.bovis_AF2122/97 VDGQLHALSPICRHR-LMLMV-----EPGAGRIDTLTCQYHLWRYGLDGR
Rv3161c|M.tuberculosis_H37Rv VDGQLHALSPICRHR-LMLMV-----EPGAGRIDTLTCQYHLWRYGLDGR
TH_3820|M.thermoresistible__bu KDGTIRAFYNLCRHRGNKLVWNDYPGEEVSGTCRQFTCKYHGWRYALNGD
Mvan_4319|M.vanbaalenii_PYR-1 KDDVLRGFVNACRHRGNALCT-----TDTGNAKRGFLCQYHLWSYDLEGK
MAV_3509|M.avium_104 SDGRVRALFNMCLHRGMQVCR------AELGNASNFRCPYHGWTYRNDGR
MAB_4370c|M.abscessus_ATCC_199 KNRRIRAFLNSCRHRGARVCR------ADYGVTRGFTCTYHGWAYDLEGK
MUL_3820|M.ulcerans_Agy99 ADRAVRAFDNVCLHRQSRVAT-------GCGTALRFTCPYHGWTYDNGGR
: ::.: * ** : : * ** * * *
Mflv_3082|M.gilvum_PYR-GCK LVAAPNIGTLTDDAGSPIDRYRYGLIPV-ALTEWLGYAWVCLSDTPPPFE
MMAR_4422|M.marinum_M LIAAPNIAELTDTDGASIDRHRYGLVAV-ALREWLGYAWVCLAEDPPSFE
MSMEG_0657|M.smegmatis_MC2_155 VRNAPGFKDVDG-----FDPGGFGLVEL-RLVDWHGWLFVDPSGQDADFA
Mb3186c|M.bovis_AF2122/97 LRGAPHMAANLD-----FNRRECRLPQF-AVATWNGLVWINLDADAEPIA
Rv3161c|M.tuberculosis_H37Rv LRGAPHMAANLD-----FNRRECRLPQF-AVATWNGLVWINLDADAEPIA
TH_3820|M.thermoresistible__bu LTFIQQEDEFFD-----VDKADYGLKPI-RCEVWEGFIFVNFDDDAEPLE
Mvan_4319|M.vanbaalenii_PYR-1 LKGLLRENLAGP-----IDKTENSLLQV-SVDTFAGFIFLNPDPDAAPLR
MAV_3509|M.avium_104 LTGLPFHREAYGGDDG-FVKDQTLLPAP-NFAGYNGLLFISLDPAAPPLQ
MAB_4370c|M.abscessus_ATCC_199 LISVPNEEGYPDS----FSTDDWGLVEVPHVDSYHGLIFGTWNPAPVGLR
MUL_3820|M.ulcerans_Agy99 LVGLPGREGFPG-----IAVGSDGLTEL-PATEFAGFLWVSLHPAARLDV
: . * : * :
Mflv_3082|M.gilvum_PYR-GCK D-VVAEATRTLGDADAINRYGIGGLDVGHRVVYEVAANWKLIVENFM-EC
MMAR_4422|M.marinum_M DDVVGSVTARLGDVSAIDTYRIEALQVGRRVSYDVAANWKLIVENFM-EC
MSMEG_0657|M.smegmatis_MC2_155 T-------HVAGLEEVVSPYRPEDLTIVARHSYELAANWKIIAENYQ-EC
Mb3186c|M.bovis_AF2122/97 A-------HLDLTDDEFAGYRLGEMVQVESWSHEWRANWKVAAENGH-EN
Rv3161c|M.tuberculosis_H37Rv A-------HLDLTDDEFAGYRLGEMVQVESWSHEWRANWKVAAENGH-EN
TH_3820|M.thermoresistible__bu D-------YLGEFGQNLKGYPFHEMTEVYSYRAEVNANWKLFIDAFT-EF
Mvan_4319|M.vanbaalenii_PYR-1 EY------LGEDVVSLLEPYNIEQFTTVMDVTEAIECNWKVVMDAFE-EG
MAV_3509|M.avium_104 DFLG----DFRFYLDYYTRQSAGGVELRGPQRWRIRANWKIGAENFAGDM
MAB_4370c|M.abscessus_ATCC_199 ESLG---DMAWYMDAMLDHDSEGTVVVGGVHKWVLDGNWKLAAEQFATDW
MUL_3820|M.ulcerans_Agy99 P------AHLGPLAGELQSWGIGRWSPLGEKVLGSPIDWKLATDTFA-EN
:**: : :
Mflv_3082|M.gilvum_PYR-GCK YHCSSIHPELVDVLPEFAKGQAA---QANIGLG--AEFGSEVAGFTVDGG
MMAR_4422|M.marinum_M YHCATIHPELTRVIPEFARGQAA---QRSVGRG--AEFGSAVAGFTVDGR
MSMEG_0657|M.smegmatis_MC2_155 YHCSSIHPELSRISPPTSGENLE---LPGAWMGGWMSIIDGAETMSLDGR
Mb3186c|M.bovis_AF2122/97 YHVLGLH---RQTLEPFVPGGGD---LDVRQYSRWALRLRVPFTVPVEAK
Rv3161c|M.tuberculosis_H37Rv YHVLGLH---RQTLEPFVPGGGD---LDVRQYSRWALRLRVPFTVPVEAK
TH_3820|M.thermoresistible__bu YHAPVLHNKQAVKEEAEKLAKYG---YEALHYDIKGPHSMISSWGGMAPP
Mvan_4319|M.vanbaalenii_PYR-1 YHINGIHPQLLQVLHINPKTARY---RFFENHSVAMAPFEVVGAGVQDQV
MAV_3509|M.avium_104 YHTPHTHASIVDIGLFREPKAQK---RKDGATYWAHRGGGTTYKLPPGGF
MAB_4370c|M.abscessus_ATCC_199 YHVNMSHASALMVMAPQGKAPKDEIVHRTGRQYVDPNGHGAGFPVHPKNR
MUL_3820|M.ulcerans_Agy99 YHFATVHRRTFATIARSN----------CTVFDSYGPHHRLIFPLNTILE
** *
Mflv_3082|M.gilvum_PYR-GCK AGFERLPGIAD----------------------------DQDRRYYAITV
MMAR_4422|M.marinum_M AGFTALPGIRP----------------------------EQDRRYFAITV
MSMEG_0657|M.smegmatis_MC2_155 SGGVAIAGLSE----------------------------HELRTVMYLVG
Mb3186c|M.bovis_AF2122/97 S-----LQLNE----------------------------VQKSNLVVLWT
Rv3161c|M.tuberculosis_H37Rv S-----LQLNE----------------------------VQKSNLVVLWT
TH_3820|M.thermoresistible__bu KDPSMVKPIER----------------------------ILNSGLFGPWD
Mvan_4319|M.vanbaalenii_PYR-1 AGILALPETFPGTVAVIPRFQELIAPYQDSDGNVAFPDGVTARALLQQAT
MAV_3509|M.avium_104 EERMRYVGYPD----------------------------EMIGRIKQVWT
MAB_4370c|M.abscessus_ATCC_199 FDARVVHQWTDYG--------------------------ALRERLGEARV
MUL_3820|M.ulcerans_Agy99 LENVPEGQWDP-----------------------------FHHMVVIYAL
Mflv_3082|M.gilvum_PYR-GCK KP--------------------------------TVFINLVPDHVIFHRM
MMAR_4422|M.marinum_M KP--------------------------------TVFINLVPDHAIIHRM
MSMEG_0657|M.smegmatis_MC2_155 YP--------------------------------NLLVSLHPDYVMTHLM
Mb3186c|M.bovis_AF2122/97 FP--------------------------------NSALAIAGERVVWFGF
Rv3161c|M.tuberculosis_H37Rv FP--------------------------------NSALAIAGERVVWFGF
TH_3820|M.thermoresistible__bu RPDIKGIAPDELPPAINPSRQPFWGQDSFEFFPNFTLLIWAPGWYLTYNY
Mvan_4319|M.vanbaalenii_PYR-1 REVLTGMG-----LDVSRLTDDQMVDNQGWVLFPNYFMTVRAGECHVIMS
MAV_3509|M.avium_104 PRQQRVVG---------------------EDGFMVSAATCFPNLSFVHNW
MAB_4370c|M.abscessus_ATCC_199 QGPLTN-----------------------GHATVFPNLSYLPVNGSVRIW
MUL_3820|M.ulcerans_Agy99 FP--------------------------------NVVLSVTIANGELFRI
Mflv_3082|M.gilvum_PYR-GCK YPVAADR-------------------TVVECDWLYTREVL-GSGRDVSRS
MMAR_4422|M.marinum_M FPIAADR-------------------TIVECDWLYAPEVA-AAG-DIAHS
MSMEG_0657|M.smegmatis_MC2_155 TPLAVDR-------------------THVECAWAFPREAAERPGFDPAYA
Mb3186c|M.bovis_AF2122/97 IPQSIDR-------------------VQVLGGVLTTPELAADAAATAQTS
Rv3161c|M.tuberculosis_H37Rv IPQSIDR-------------------VQVLGGVLTTPELAADAAATAQTS
TH_3820|M.thermoresistible__bu WPTDVTK-------------------HIFEASLYFVPPKTTRQRLAQELA
Mvan_4319|M.vanbaalenii_PYR-1 VPHPDGDPN---------------RCIWHVASYMYVPQDFRDAVRAEALV
MAV_3509|M.avium_104 PKVRDGRDDETLPFISIRLWQPISENETEVCSWFAVDSAAPAHYKQDSYK
MAB_4370c|M.abscessus_ATCC_199 HPKGPDK--------------------MEVWAWTIVDRSMPEEVRDAQRL
MUL_3820|M.ulcerans_Agy99 YPGDRPG------------------SSITVHQNSTPLDLSDESVATGAQA
Mflv_3082|M.gilvum_PYR-GCK VELFHRVN-----MQDFDACERTQPAMSS------RAYRSGGVLVPAEHH
MMAR_4422|M.marinum_M VELFHRVN-----EQDFDACQRTQPAMSS------RAYRTGGVLVPAEHH
MSMEG_0657|M.smegmatis_MC2_155 VDFWDLTN-----RQDWAACESVQRGLSS------PHARPG-PLAPDEDG
Mb3186c|M.bovis_AF2122/97 QFVMAMIN-----DEDRLGLEAVQVGAGS------RFAERGHLSSKEWPG
Rv3161c|M.tuberculosis_H37Rv QFVMAMIN-----DEDRLGLEAVQVGAGS------RFAERGHLSSKEWPG
TH_3820|M.thermoresistible__bu AVTFKEYA-----LQDANTLEATQTQIGTRAVTEFPLCDQEVLLRHLHHT
Mvan_4319|M.vanbaalenii_PYR-1 VDTPGSYKYFEALQQDYEQMPRQQKGLRNDRLDHMSLVKEEVVIAHYHSV
MAV_3509|M.avium_104 AYLMCFGSTGMFEQDDVENWVSLTTTAGGSMARRLLLNSRMGLYDDGRPV
MAB_4370c|M.abscessus_ATCC_199 YNLRTFGPTGIFEQDDGENWSEVQSISRGFVTNSVALNYQMGLDSEREDG
MUL_3820|M.ulcerans_Agy99 VFDYAHATVR---DEDYRLAETLQTNLESG-------ARQRLVFGRNEPG
:*
Mflv_3082|M.gilvum_PYR-GCK LAEFHRWVVSRLATPV----------------------------
MMAR_4422|M.marinum_M IAEFHQWVIARIGTPAVTG-------------------------
MSMEG_0657|M.smegmatis_MC2_155 VYQFVTRVARAYQGA-----------------------------
Mb3186c|M.bovis_AF2122/97 MLAFYRNLAMALVGDHPGAS------------------------
Rv3161c|M.tuberculosis_H37Rv MLAFYRNLAMALVGDHPGAS------------------------
TH_3820|M.thermoresistible__bu AHQYVDKYKEELAQKAQQTNGSKKEGARSTAHV-----------
Mvan_4319|M.vanbaalenii_PYR-1 VDRHLADSVTADTARSTSSVKESRD-------------------
MAV_3509|M.avium_104 VEALAPSAFHGPGRAQVGYNEHNQRALLAMWADYLQEGDACENR
MAB_4370c|M.abscessus_ATCC_199 RHPGLTSELYSDAAGRSFYARWRDLMNTKAWHEEHV--------
MUL_3820|M.ulcerans_Agy99 LQHRHITWAQALETPG----------------------------