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DHFPAADYPDCMFKQIGPAPVPADDLAAALAPFGHSRMLPREAYVDPAVFAWEQQAIFSGWTCVGHAADL ETPGAQRAVGSGANGVLLVRGEDNRVRAFANACRHRGHELLSCGASAKRRSIVCPYHAWSYRLDGSVRNA PGFKDVDGFDPGGFGLVELRLVDWHGWLFVDPSGQDADFATHVAGLEEVVSPYRPEDLTIVARHSYELAA NWKIIAENYQECYHCSSIHPELSRISPPTSGENLELPGAWMGGWMSIIDGAETMSLDGRSGGVAIAGLSE HELRTVMYLVGYPNLLVSLHPDYVMTHLMTPLAVDRTHVECAWAFPREAAERPGFDPAYAVDFWDLTNRQ DWAACESVQRGLSSPHARPGPLAPDEDGVYQFVTRVARAYQGA*
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. smegmatis MC2 155 | MSMEG_0657 | - | - | 100% (394) | Rieske (2Fe-2S) domain-containing protein |
| M. smegmatis MC2 155 | MSMEG_0299 | - | 1e-19 | 29.76% (205) | Rieske (2Fe-2S) domain-containing protein |
| M. smegmatis MC2 155 | MSMEG_4840 | - | 2e-19 | 30.65% (199) | Rieske (2Fe-2S) domain-containing protein |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb3186c | - | 2e-25 | 25.56% (356) | dioxygenase |
| M. gilvum PYR-GCK | Mflv_3082 | - | 5e-56 | 37.01% (354) | Rieske (2Fe-2S) domain-containing protein |
| M. tuberculosis H37Rv | Rv3161c | - | 4e-25 | 25.28% (356) | dioxygenase |
| M. leprae Br4923 | - | - | - | - | - |
| M. abscessus ATCC 19977 | MAB_4370c | - | 4e-18 | 28.37% (215) | putative large terminal subunit of phenylpropionate dioxygenase |
| M. marinum M | MMAR_4422 | - | 5e-61 | 39.83% (354) | dioxygenase |
| M. avium 104 | MAV_5278 | - | 5e-27 | 32.08% (212) | Rieske (2Fe-2S) domain-containing protein |
| M. thermoresistible (build 8) | TH_4380 | - | 4e-22 | 26.67% (225) | PUTATIVE putative Rieske (2Fe-2S) domain protein |
| M. ulcerans Agy99 | MUL_4884 | - | 3e-20 | 29.45% (343) | dioxygenase |
| M. vanbaalenii PYR-1 | Mvan_4319 | - | 8e-24 | 28.96% (221) | Rieske (2Fe-2S) domain-containing protein |
CLUSTAL 2.0.9 multiple sequence alignment
Mflv_3082|M.gilvum_PYR-GCK --------------------------------MTALMATLEGHYYTSAEV
MMAR_4422|M.marinum_M --------------------------MTAVRQPSALLPTLGGAYYTSAAV
MSMEG_0657|M.smegmatis_MC2_155 MDHFPAADYPDCMFKQIGPAPVPADDLAAALAPFGHSRMLPREAYVDPAV
Mb3186c|M.bovis_AF2122/97 ----------MLSTDNRAELGDILTDIGDYLDDNPPALSLPPAAYTSSEL
Rv3161c|M.tuberculosis_H37Rv ----------MLSTDNRAELGDILTDIGDYLDDNPPALSLPPAAYTSSEL
MAV_5278|M.avium_104 -------------MAAAAGNPARTRDEDAIGTPPPGPTLVPAERYYSPAF
TH_4380|M.thermoresistible__bu --------------------MTDLTAQRVPQKYACGETFVPKSRYIDPEF
Mvan_4319|M.vanbaalenii_PYR-1 --------MPATRPGEWLDPASGLGLGLDDVQPGTFNMTISTDRYTCPDY
MUL_4884|M.ulcerans_Agy99 ----MSSPTKPLVAEDAEIVRRVLAHIDAGTTDEGEAWREPVENYASPER
MAB_4370c|M.abscessus_ATCC_199 --MASLPRPVAVAQGALCRPQALVRDIDVSGLVDSHGGLLDRVIFTDGEL
:
Mflv_3082|M.gilvum_PYR-GCK FAAEQQGIFENMWFCAVRTSDLEKPGKFTKVQVGRESVLLVRGRDGLLRA
MMAR_4422|M.marinum_M FAAEQQHVFESMWFCVVRAADLNEPGQFKAVQVGRESVLLVRGRDRRLRA
MSMEG_0657|M.smegmatis_MC2_155 FAWEQQAIFS-GWTCVGHAADLETPGAQRAVGSGANGVLLVRGEDNRVRA
Mb3186c|M.bovis_AF2122/97 WQLERERIFNRSWMLVAHVDQLAKTGDYVTVSVAGEPVMVVRDVDGQLHA
Rv3161c|M.tuberculosis_H37Rv WQLERERIFNRSWMLVAHVDQVAKTGDYVTVSVAGEPVMVVRDVDGQLHA
MAV_5278|M.avium_104 AALEVERMWPRVWQLACMVDHVATPGDYFEYRCGPYGVLIVRDDDGALRA
TH_4380|M.thermoresistible__bu LQLELDRLFTQVWQPACREEEIPDPGSYYEYCIGRQSIMVVRQKDRSIKA
Mvan_4319|M.vanbaalenii_PYR-1 AARERGSIWMRTWQIAGRVEDLPKPGDWKKYEILDQSFIIVRGKDDVLRG
MUL_4884|M.ulcerans_Agy99 FAAELR-LLRGMPSVFVPSAAIPNNGDHVERTAFGVPLFAVRGADGRARV
MAB_4370c|M.abscessus_ATCC_199 YRQELRRVFAPSWLFLAHADQFHRPGDFFTTYMGEDPVIVSMDKNRRIRA
* : . * .: : :
Mflv_3082|M.gilvum_PYR-GCK FLNVCRHRGAQLCSESEGEVRRTLRCPYHSWTYALDGKLVAAPNIGTLTD
MMAR_4422|M.marinum_M FLNICRHRGALLCTEPEGQVRRNLRCPYHSWTYGLDGTLIAAPNIAELTD
MSMEG_0657|M.smegmatis_MC2_155 FANACRHRGHELLSCGASAKRRSIVCPYHAWSYRLDGSVRNAPGFKDVDG
Mb3186c|M.bovis_AF2122/97 LSPICRHR-LMLMVEPGAGRIDTLTCQYHLWRYGLDGRLRGAPHMAANLD
Rv3161c|M.tuberculosis_H37Rv LSPICRHR-LMLMVEPGAGRIDTLTCQYHLWRYGLDGRLRGAPHMAANLD
MAV_5278|M.avium_104 FQNVCRHRGNSLCSGSGSG-LRELKCGYHGWTWDLAGALKRVPDRKGFGS
TH_4380|M.thermoresistible__bu FHNTCAHRGMKIVCGTGVEAEGELRCRFHGWRYSLDGRSSFVPGREEFAN
Mvan_4319|M.vanbaalenii_PYR-1 FVNACRHRGNALCTTDTGNAKRGFLCQYHLWSYDLEGKLKGLLRENLAGP
MUL_4884|M.ulcerans_Agy99 FRNARRHRGFALVEN--AGCSHALVCRYHGWIYRLDGSLSHVPHAEAFPD
MAB_4370c|M.abscessus_ATCC_199 FLNSCRHRGARVCRAD-YGVTRGFTCTYHGWAYDLEGKLISVPNEEGYPD
: ** : : * :* * : * *
Mflv_3082|M.gilvum_PYR-GCK DAGSPIDRYRYGLIPVALTEWLGYAWVC-LSDTPPPFED-VVAEATRTLG
MMAR_4422|M.marinum_M TDGASIDRHRYGLVAVALREWLGYAWVC-LAEDPPSFEDDVVGSVTARLG
MSMEG_0657|M.smegmatis_MC2_155 -----FDPGGFGLVELRLVDWHGWLFVD-PSGQDADFAT-------HVAG
Mb3186c|M.bovis_AF2122/97 -----FNRRECRLPQFAVATWNGLVWIN-LDADAEPIAA-------HLDL
Rv3161c|M.tuberculosis_H37Rv -----FNRRECRLPQFAVATWNGLVWIN-LDADAEPIAA-------HLDL
MAV_5278|M.avium_104 -----LRLSDYPLIPARVDTWAGLVFVN-LDPDAMPLPE-------YLEA
TH_4380|M.thermoresistible__bu -----RPREQWGLRPVHVGTWGGWVFIS-MADEPPELLE-------WLDP
Mvan_4319|M.vanbaalenii_PYR-1 -----IDKTENSLLQVSVDTFAGFIFLN-PDPDAAPLREY------LGED
MUL_4884|M.ulcerans_Agy99 -----LDMSARGLVEVESHEVDGLIVIGPLDPVDAGLIEDEGAIGWLTDG
MAB_4370c|M.abscessus_ATCC_199 S----FSTDDWGLVEVPHVDSYHGLIFGTWNPAPVGLRESLG---DMAWY
* . :
Mflv_3082|M.gilvum_PYR-GCK DADAINRYGIGGLDVGHRVVYEVAANWKLIVENFM-ECYHCSSIHPELVD
MMAR_4422|M.marinum_M DVSAIDTYRIEALQVGRRVSYDVAANWKLIVENFM-ECYHCATIHPELTR
MSMEG_0657|M.smegmatis_MC2_155 LEEVVSPYRPEDLTIVARHSYELAANWKIIAENYQ-ECYHCSSIHPELSR
Mb3186c|M.bovis_AF2122/97 TDDEFAGYRLGEMVQVESWSHEWRANWKVAAENGH-ENYHVLGLH---RQ
Rv3161c|M.tuberculosis_H37Rv TDDEFAGYRLGEMVQVESWSHEWRANWKVAAENGH-ENYHVLGLH---RQ
MAV_5278|M.avium_104 IPEDIAWCRLDEFRCYATLTVEVDANWKTIADGYS-ETYHIQTLHPELLR
TH_4380|M.thermoresistible__bu LPTALAPFRLQDMRFRWRKRTTLPANYKTVIDAFI-EGYHTPGTHPQTLR
Mvan_4319|M.vanbaalenii_PYR-1 VVSLLEPYNIEQFTTVMDVTEAIECNWKVVMDAFE-EGYHINGIHPQLLQ
MUL_4884|M.ulcerans_Agy99 SPWRAKLVPAERLIVVESTLREIN--WKVLVEQFL-EGYHIRSTHKDTFF
MAB_4370c|M.abscessus_ATCC_199 MDAMLDHDSEGTVVVGGVHKWVLDGNWKLAAEQFATDWYHVNMSHASALM
. :* : : ** *
Mflv_3082|M.gilvum_PYR-GCK VLPEFAK-------GQAAQANIGLG--AEFGSEVAG--------------
MMAR_4422|M.marinum_M VIPEFAR-------GQAAQRSVGRG--AEFGSAVAG--------------
MSMEG_0657|M.smegmatis_MC2_155 ISPPTSG-------ENLELPGAWMGGWMSIIDGAET--------------
Mb3186c|M.bovis_AF2122/97 TLEPFVP-------GGGDLDVRQYSRWALRLRVPFT--------------
Rv3161c|M.tuberculosis_H37Rv TLEPFVP-------GGGDLDVRQYSRWALRLRVPFT--------------
MAV_5278|M.avium_104 CVDDIHAPQ--QIWRHTGKSDQPYGVPSPRFEGALSDEEVWDAYVSTQGA
TH_4380|M.thermoresistible__bu TAQSPHPST--EPAKPQEYVYAPYTPTIAYKNHSRFIYTQRPE-------
Mvan_4319|M.vanbaalenii_PYR-1 VLHINPKTARYRFFENHSVAMAPFEVVGAGVQDQVAGILALPETFPGTVA
MUL_4884|M.ulcerans_Agy99 PVQYDDLN--------VVEAFGPNTRITFPYRNIER--------------
MAB_4370c|M.abscessus_ATCC_199 VMAPQGKAPKDEIVHRTGRQYVDPNGHGAGFPVHPK--------------
Mflv_3082|M.gilvum_PYR-GCK ---------FTVDGGAGFERL------PGIADDQDRRYYAIT------VK
MMAR_4422|M.marinum_M ---------FTVDGRAGFTAL------PGIRPEQDRRYFAIT------VK
MSMEG_0657|M.smegmatis_MC2_155 ---------MSLDGRSGGVAI------AGLSEHELRTVMYLV------GY
Mb3186c|M.bovis_AF2122/97 ---------VPVEAKS-----------LQLNEVQKSNLVVLW------TF
Rv3161c|M.tuberculosis_H37Rv ---------VPVEAKS-----------LQLNEVQKSNLVVLW------TF
MAV_5278|M.avium_104 LMGAAEGTPFPAADRRPGQTV------ADLIADRTRAFAATRGVDLGWAD
TH_4380|M.thermoresistible__bu ---TAERDKGRQEQASRPEVF------ANAMQYNYIGVNALVTER-DYRA
Mvan_4319|M.vanbaalenii_PYR-1 VIPRFQELIAPYQDSDGNVAFPDGVTARALLQQATREVLTGMGLDVSRLT
MUL_4884|M.ulcerans_Agy99 -----------LRDRP-----------EHTWTTGARVTYLYQ------LF
MAB_4370c|M.abscessus_ATCC_199 ---------NRFDARVVHQWTDYGALRERLGEARVQGPLTNG-----HAT
Mflv_3082|M.gilvum_PYR-GCK PTVFINLVPDHVIFHRMYPVAADRTVVECDWLYT----------------
MMAR_4422|M.marinum_M PTVFINLVPDHAIIHRMFPIAADRTIVECDWLYA----------------
MSMEG_0657|M.smegmatis_MC2_155 PNLLVSLHPDYVMTHLMTPLAVDRTHVECAWAFP----------------
Mb3186c|M.bovis_AF2122/97 PNSALAIAGERVVWFGFIPQSIDRVQVLGGVLTT----------------
Rv3161c|M.tuberculosis_H37Rv PNSALAIAGERVVWFGFIPQSIDRVQVLGGVLTT----------------
MAV_5278|M.avium_104 TDRITRLHQYNVFPNMTFLTNADHLTVMCSRPAP----------------
TH_4380|M.thermoresistible__bu AQKLATMEPSEVPPFVLYHQLCEELALAEGVDFPRMSMEEYFAGNGDWHI
Mvan_4319|M.vanbaalenii_PYR-1 DDQMVDNQGWVLFPNYFMTVRAGECHVIMSVPHP----------------
MUL_4884|M.ulcerans_Agy99 PNVMLATFPDVIAMVVIDPVSVDRTAVTIYSMVT----------------
MAB_4370c|M.abscessus_ATCC_199 VFPNLSYLPVNGSVRIWHPKGPDKMEVWAWTIVDR---------------
. :
Mflv_3082|M.gilvum_PYR-GCK -------------------REVL---------------GSGRDVSRSVEL
MMAR_4422|M.marinum_M -------------------PEVA---------------AAG-DIAHSVEL
MSMEG_0657|M.smegmatis_MC2_155 -------------------REAAE--------------RPGFDPAYAVDF
Mb3186c|M.bovis_AF2122/97 -------------------PELAA--------------DAAATAQTSQFV
Rv3161c|M.tuberculosis_H37Rv -------------------PELAA--------------DAAATAQTSQFV
MAV_5278|M.avium_104 -------------------DPAAAPDKGELVMFLTTRMPPGAPRTKPTDV
TH_4380|M.thermoresistible__bu FPTMVILVEKSCLLGYRMLPDAEDPDKCTFEMFTLEHFAPGEVPETSWQV
Mvan_4319|M.vanbaalenii_PYR-1 --------------------DGDPNRCIWHVASYMYVPQDFRDAVRAEAL
MUL_4884|M.ulcerans_Agy99 -------------------PDVAE--------------RDSLDASSNLAG
MAB_4370c|M.abscessus_ATCC_199 -------------------SMPEEVRDAQRLYNLRTFGPTGIFEQDDGEN
Mflv_3082|M.gilvum_PYR-GCK FHRVNMQD-----FDACERTQPAMSSRAYRSGG---VLVPAEHHLAEFHR
MMAR_4422|M.marinum_M FHRVNEQD-----FDACQRTQPAMSSRAYRTGG---VLVPAEHHIAEFHQ
MSMEG_0657|M.smegmatis_MC2_155 WDLTNRQD-----WAACESVQRGLSSPHARPG----PLAPDEDGVYQFVT
Mb3186c|M.bovis_AF2122/97 MAMINDED-----RLGLEAVQVGAGSRFAERGH---LSSKEWPGMLAFYR
Rv3161c|M.tuberculosis_H37Rv MAMINDED-----RLGLEAVQVGAGSRFAERGH---LSSKEWPGMLAFYR
MAV_5278|M.avium_104 RMSAGEAEPG-LVLTQDIAVLAGLQRGMHQPGFTHLVLSSEERRVINMHR
TH_4380|M.thermoresistible__bu FERWQDHDGWGELPTQDLQNIEAIHAGMHSHGFEGLWLNTAQEMSIRNEH
Mvan_4319|M.vanbaalenii_PYR-1 VVDTPGSYKYFEALQQDYEQMPRQQKGLRNDRLDHMSLVKEEVVIAHYHS
MUL_4884|M.ulcerans_Agy99 AGSFIGQG-----LFEDNEMSEGVQRGLAAGANAFVEFGRHESAVGRFHA
MAB_4370c|M.abscessus_ATCC_199 WSEVQSISRGFVTNSVALNYQMGLDSEREDGRHPGLTSELYSDAAGRSFY
Mflv_3082|M.gilvum_PYR-GCK WVVSRLATPV----------------
MMAR_4422|M.marinum_M WVIARIGTPAVTG-------------
MSMEG_0657|M.smegmatis_MC2_155 RVARAYQGA-----------------
Mb3186c|M.bovis_AF2122/97 NLAMALVGDHPGAS------------
Rv3161c|M.tuberculosis_H37Rv NLAMALVGDHPGAS------------
MAV_5278|M.avium_104 NLERYLDLPAAQRMSGGAGT------
TH_4380|M.thermoresistible__bu AIADRFIFGLDKQ-------------
Mvan_4319|M.vanbaalenii_PYR-1 VVDRHLADSVTADTARSTSSVKESRD
MUL_4884|M.ulcerans_Agy99 ALGRRLARLTSAG-------------
MAB_4370c|M.abscessus_ATCC_199 ARWRDLMNTKAWHEEHV---------