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MPATRPGEWVDADSGLGLGLDDVAPGTFNMTIPTDRYTCPDYAAREREAIWTRIWQIAGRAEDIPKPGDW KRYEILDQSFIIVRGKDEQLRGFVNACRHRGNALCVTDTGNAKRGFLCQYHLWSYDLEGTLKGLLRENLA GSIDKAENSLLPVSVDTFAGFIFLNPDPHAAPLREYLGEEVVSLLEPYNIEQFTTVMDVTEAIECNWKVV MDAFEEGYHINGIHPQLLQVLHINPKTARYRFFESHSVAMAPFEVVGAGVQDQVAGILALPETFPGTVAV IPRFQELIAPYQDEDGNVDFPDGLTARLLLQQATREVLTGMGLDVSGLTDSQMVDNQGWVLFPNYFMTVR AGECHVIMSRPHPSGDPNRCIWHVASYMYVPQEFRDAVRAESIVVDTPGSHKYFEALQQDYEQMPRQQKG LRNDRLDHMSLVKEEVVIAHYHSVVDRYMAASEVKA
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. gilvum PYR-GCK | Mflv_2328 | - | - | 100% (456) | Rieske (2Fe-2S) domain-containing protein |
| M. gilvum PYR-GCK | Mflv_1788 | - | 4e-42 | 27.44% (441) | Rieske (2Fe-2S) domain-containing protein |
| M. gilvum PYR-GCK | Mflv_2464 | - | 8e-34 | 28.11% (466) | Rieske (2Fe-2S) domain-containing protein |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb3186c | - | 1e-22 | 29.28% (222) | dioxygenase |
| M. tuberculosis H37Rv | Rv3161c | - | 1e-22 | 29.28% (222) | dioxygenase |
| M. leprae Br4923 | - | - | - | - | - |
| M. abscessus ATCC 19977 | MAB_4370c | - | 8e-20 | 30.59% (219) | putative large terminal subunit of phenylpropionate dioxygenase |
| M. marinum M | MMAR_0307 | - | 1e-43 | 28.78% (417) | dioxygenase |
| M. avium 104 | MAV_5278 | - | 6e-44 | 28.40% (426) | Rieske (2Fe-2S) domain-containing protein |
| M. smegmatis MC2 155 | MSMEG_5627 | - | 2e-38 | 25.23% (428) | Rieske (2Fe-2S) domain-containing protein |
| M. thermoresistible (build 8) | TH_1336 | - | 8e-37 | 24.88% (422) | Rieske |
| M. ulcerans Agy99 | MUL_3820 | - | 1e-16 | 29.27% (205) | ring-hydroxylating dioxygenase, large terminal subunit |
| M. vanbaalenii PYR-1 | Mvan_4319 | - | 0.0 | 91.81% (452) | Rieske (2Fe-2S) domain-containing protein |
CLUSTAL 2.0.9 multiple sequence alignment
Mb3186c|M.bovis_AF2122/97 -------MLSTDNRAELGDILTDIGDYLDDNPP--ALSLPPAAYTSSELW
Rv3161c|M.tuberculosis_H37Rv -------MLSTDNRAELGDILTDIGDYLDDNPP--ALSLPPAAYTSSELW
Mflv_2328|M.gilvum_PYR-GCK -------MPATRPGEWVDADSGLGLGLDDVAPGTFNMTIPTDRYTCPDYA
Mvan_4319|M.vanbaalenii_PYR-1 -------MPATRPGEWLDPASGLGLGLDDVQPGTFNMTISTDRYTCPDYA
MSMEG_5627|M.smegmatis_MC2_155 -------MTDTQPR------------TDEALAT--PMTIGVEAYTSEAYA
TH_1336|M.thermoresistible__bu -------MTDIDNKPEV--------AAPEELSE--PMLIPVEAYVSEEYA
MMAR_0307|M.marinum_M -------MTDHNDGRAEPMDAAHHDDVEATLTG--AVTFGVDAYTCADYA
MAV_5278|M.avium_104 -------MAAAAGNPARTRDED---AIGTPPPG--PTLVPAERYYSPAFA
MUL_3820|M.ulcerans_Agy99 -------MDRDQLIDLTRRALKLARDKTTDLAP-HQHAVDAREYTSLDRH
MAB_4370c|M.abscessus_ATCC_199 MASLPRPVAVAQGALCRPQALVRDIDVSGLVDS-HGGLLDRVIFTDGELY
: . :
Mb3186c|M.bovis_AF2122/97 QLERERIFNRSWMLVAHVDQLAKTGDYVTVSVAGEPVMVVRD----VDGQ
Rv3161c|M.tuberculosis_H37Rv QLERERIFNRSWMLVAHVDQVAKTGDYVTVSVAGEPVMVVRD----VDGQ
Mflv_2328|M.gilvum_PYR-GCK AREREAIWTRIWQIAGRAEDIPKPGDWKRYEILDQSFIIVRG----KDEQ
Mvan_4319|M.vanbaalenii_PYR-1 ARERGSIWMRTWQIAGRVEDLPKPGDWKKYEILDQSFIIVRG----KDDV
MSMEG_5627|M.smegmatis_MC2_155 RAERDRLWRRVWQQVGRVEELPEVGSYLTYDILDDSILVVRTGPGDSGGS
TH_1336|M.thermoresistible__bu RAERDRLWRKVWLQVGRVEELPEVGSYLTYDILDDSIIVVRTGAGDSGSA
MMAR_0307|M.marinum_M RAERDKLWRKVWQQVGRVEDLPKVGSYLTYDILDDSIVVVRS----AADT
MAV_5278|M.avium_104 ALEVERMWPRVWQLACMVDHVATPGDYFEYRCGPYGVLIVRD----DDGA
MUL_3820|M.ulcerans_Agy99 ERDRAMVLD-SPQLVGYSSELPGPDTYRTKTIMGRSILLTRA----ADRA
MAB_4370c|M.abscessus_ATCC_199 RQELRRVFAPSWLFLAHADQFHRPGDFFTTYMGEDPVIVSMD----KNRR
: : ... . : .::
Mb3186c|M.bovis_AF2122/97 LHALSPICRHRLMLMVEPGAGR-------IDTLTCQYHLWRYGLDGRLRG
Rv3161c|M.tuberculosis_H37Rv LHALSPICRHRLMLMVEPGAGR-------IDTLTCQYHLWRYGLDGRLRG
Mflv_2328|M.gilvum_PYR-GCK LRGFVNACRHRGNALCVTDTGN------AKRGFLCQYHLWSYDLEGTLKG
Mvan_4319|M.vanbaalenii_PYR-1 LRGFVNACRHRGNALCTTDTGN------AKRGFLCQYHLWSYDLEGKLKG
MSMEG_5627|M.smegmatis_MC2_155 FAAHHNVCMHRGRRLVDTPAGAKNACARTRKSFMCGFHGWTYDLTGACTH
TH_1336|M.thermoresistible__bu FAAHHNVCMHRGRRLIDTPEGAKNACGRTRRSFVCGFHGWTYGLDGACTH
MMAR_0307|M.marinum_M FKAHHNVCMHRGRRLVDIPPGAKNVCG-HKKSFVCGFHGWTYDQDGNCIH
MAV_5278|M.avium_104 LRAFQNVCRHRGNSLCSGSGSG-------LRELKCGYHGWTWDLAGALKR
MUL_3820|M.ulcerans_Agy99 VRAFDNVCLHRQSRVATGCGTA--------LRFTCPYHGWTYDNGGRLVG
MAB_4370c|M.abscessus_ATCC_199 IRAFLNSCRHRGARVCRADYGVT-------RGFTCTYHGWAYDLEGKLIS
. . * ** : : * :* * :. *
Mb3186c|M.bovis_AF2122/97 APHMAANL-DFNRRECRLPQFA-VATWNGLVWINLDADAEPIAAHLD---
Rv3161c|M.tuberculosis_H37Rv APHMAANL-DFNRRECRLPQFA-VATWNGLVWINLDADAEPIAAHLD---
Mflv_2328|M.gilvum_PYR-GCK LLR-ENLAGSIDKAENSLLPVS-VDTFAGFIFLNPDPHAAPLREYLG---
Mvan_4319|M.vanbaalenii_PYR-1 LLR-ENLAGPIDKTENSLLQVS-VDTFAGFIFLNPDPDAAPLREYLG---
MSMEG_5627|M.smegmatis_MC2_155 IREQDDWQGTLTPENTHLPRVH-VDTWGGWLFINMDPDPEPLADYL----
TH_1336|M.thermoresistible__bu IREQDDWKGALTPENTRLAPVN-VDTWGGWLWINMDPACEPLADYL----
MMAR_0307|M.marinum_M IPEKPDWQGALTPENTHLAAVN-VDTWGGWIWINMDPAAQPLRQYL----
MAV_5278|M.avium_104 VPDRKGFG-SLRLSDYPLIPAR-VDTWAGLVFVNLDPDAMPLPEYL----
MUL_3820|M.ulcerans_Agy99 LPGREGFP-GIAVGSDGLTELP-ATEFAGFLWVSLHPAARLDVPAHL---
MAB_4370c|M.abscessus_ATCC_199 VPNEEGYPDSFSTDDWGLVEVPHVDSYHGLIFGTWNPAPVGLRESLGDMA
: . * . : * :: . ..
Mb3186c|M.bovis_AF2122/97 LTDDEFAG-YRLGEMVQVESWSHEWRANWKVAAENGH-ENYHVLGLHRQT
Rv3161c|M.tuberculosis_H37Rv LTDDEFAG-YRLGEMVQVESWSHEWRANWKVAAENGH-ENYHVLGLHRQT
Mflv_2328|M.gilvum_PYR-GCK EEVVSLLEPYNIEQFTTVMDVTEAIECNWKVVMDAFE-EGYHINGIHPQL
Mvan_4319|M.vanbaalenii_PYR-1 EDVVSLLEPYNIEQFTTVMDVTEAIECNWKVVMDAFE-EGYHINGIHPQL
MSMEG_5627|M.smegmatis_MC2_155 FPAAKILDPFGLENMRYKWRKWLYFDCNWKVALEAFN-ETYHVFTTHPEF
TH_1336|M.thermoresistible__bu FPADKLLDHFGLENMRYKWRRWLDFDCNWKVALEAFN-ETYHVYTTHPEF
MMAR_0307|M.marinum_M EPAATMLDPFRLQDMRCKWRKWLTFQCNWKVAMEAFN-ETYHVATTHPQF
MAV_5278|M.avium_104 EAIPEDIAWCRLDEFRCYATLTVEVDANWKTIADGYS-ETYHIQTLHPEL
MUL_3820|M.ulcerans_Agy99 GPLAGELQSWGIGRWSPLGEKVLGSPIDWKLATDTFA-ENYHFATVHRRT
MAB_4370c|M.abscessus_ATCC_199 WYMDAMLDHDSEGTVVVGGVHKWVLDGNWKLAAEQFATDWYHVNMSHASA
:** : : **. *
Mb3186c|M.bovis_AF2122/97 LEPFVPG-----------------------------GGDLD-VRQYSRWA
Rv3161c|M.tuberculosis_H37Rv LEPFVPG-----------------------------GGDLD-VRQYSRWA
Mflv_2328|M.gilvum_PYR-GCK LQVLHIN------PKTARYRFFESHSVAMAPFEV-VGAGVQ-DQVAGILA
Mvan_4319|M.vanbaalenii_PYR-1 LQVLHIN------PKTARYRFFENHSVAMAPFEV-VGAGVQ-DQVAGILA
MSMEG_5627|M.smegmatis_MC2_155 NKFGEFKGWAKAQGKHSNIGYDAPKDMEATKSKIRLGTGDD-PRVSTAEM
TH_1336|M.thermoresistible__bu NRFGEFKGWAKAQGRHSNIGYDAPKDLEATKSKIRLGTGDD-PRVSTAEM
MMAR_0307|M.marinum_M NKFGNFRGWAAAHGRHSNIGYDAPKDLAQTKSKIRLGTGAD-PRVSTAEM
MAV_5278|M.avium_104 LRCVDDIH--------------APQQIWRHTGKSDQPYGVPSPRFEGALS
MUL_3820|M.ulcerans_Agy99 FATIARSN----------------------------------CTVFDSYG
MAB_4370c|M.abscessus_ATCC_199 LMVMAPQG--------------------------------KAPKDEIVHR
Mb3186c|M.bovis_AF2122/97 LRLRVPFTVPVEAKSLQLNEVQ--------------------------KS
Rv3161c|M.tuberculosis_H37Rv LRLRVPFTVPVEAKSLQLNEVQ--------------------------KS
Mflv_2328|M.gilvum_PYR-GCK LPETFPGTVAVIPRFQELIAPYQDEDGNVDFPDGLTARLLLQQATREVLT
Mvan_4319|M.vanbaalenii_PYR-1 LPETFPGTVAVIPRFQELIAPYQDSDGNVAFPDGVTARALLQQATREVLT
MSMEG_5627|M.smegmatis_MC2_155 QMYTWEQTNATTTETLVNAAKRLVDELPEGTPPDKVLEHWLASARRDDEA
TH_1336|M.thermoresistible__bu QMYTWEQTNATTTETLVNAAKRLVDELPEGTPPDKVLEHWLASARRDDEA
MMAR_0307|M.marinum_M QRYTLEETNATTTRTLVDAALRLVEELPEDAPAGQVLDHWLTSARRDDAA
MAV_5278|M.avium_104 DEEVWDAYVSTQGALMGAAEGTPFP--AADRRPGQTVADLIADRTRAFAA
MUL_3820|M.ulcerans_Agy99 PHHRLIFPLNTILELENVPEGQWDP-------------------FHHMVV
MAB_4370c|M.abscessus_ATCC_199 TGRQYVDPNGHGAGFPVHPKNRFDARVVHQWTDYGALRERLGEARVQGPL
Mb3186c|M.bovis_AF2122/97 NLVVLWTFP-NSALAIAGERVVWFG--FIPQSIDRVQVLGG---------
Rv3161c|M.tuberculosis_H37Rv NLVVLWTFP-NSALAIAGERVVWFG--FIPQSIDRVQVLGG---------
Mflv_2328|M.gilvum_PYR-GCK GMGLDVSGL-TDSQMVDNQGWVLFPNYFMTVRAGECHVIMSRPHPSGDPN
Mvan_4319|M.vanbaalenii_PYR-1 GMGLDVSRL-TDDQMVDNQGWVLFPNYFMTVRAGECHVIMSVPHPDGDPN
MSMEG_5627|M.smegmatis_MC2_155 RGVIWPTIP-ADILGQAGTAWQIFPNFQIGQGLTTALCYSAKPHPSYNPD
TH_1336|M.thermoresistible__bu RGVIWPTIP-PEVLGEAGTAWQIFPNFQIGQGLTTALCYSARPHPSYDPN
MMAR_0307|M.marinum_M RGVLWPTID-PEHLAQSGTAWQIFPNFQIGQGLTTALCYSARPHG-YDPD
MAV_5278|M.avium_104 TRGVDLGWA-DTDRITRLHQYNVFPNMTFLTNADHLTVMCSRPAPDPAAA
MUL_3820|M.ulcerans_Agy99 IYALFPNVV-LSVTIANGELFRIYPGDRPGSSITVHQNSTP---------
MAB_4370c|M.abscessus_ATCC_199 TNGHATVFPNLSYLPVNGSVRIWHPKGPDKMEVWAWTIVDRSMPEEVRDA
.
Mb3186c|M.bovis_AF2122/97 --VLTTPELAADAAATAQTSQFVMAMINDE-----------DRLGLEAVQ
Rv3161c|M.tuberculosis_H37Rv --VLTTPELAADAAATAQTSQFVMAMINDE-----------DRLGLEAVQ
Mflv_2328|M.gilvum_PYR-GCK RCIWHVASYMYVPQEFRDAVRAESIVVDTPGSHKYFEALQQDYEQMPRQQ
Mvan_4319|M.vanbaalenii_PYR-1 RCIWHVASYMYVPQDFRDAVRAEALVVDTPGSYKYFEALQQDYEQMPRQQ
MSMEG_5627|M.smegmatis_MC2_155 KCIFEVATFELFPKGQEPRTEWEYTPADSP---NWLSVLPQDFSNMAAVQ
TH_1336|M.thermoresistible__bu KCLFEVSVLELFPKGEEPQTEWEYTPVGDP---RWLSVLPQDFSNMAAVQ
MMAR_0307|M.marinum_M QCVFEASCYELYPKGEEPHTEWVYAPPDDP---GWRSVLPQDFSNMAAVQ
MAV_5278|M.avium_104 PDKGELVMFLTTRMPPGAPRTKPTDVRMSAGEAEPGLVLTQDIAVLAGLQ
MUL_3820|M.ulcerans_Agy99 ----LDLSDESVATGAQAVFDYAHATVRDE-----------DYRLAETLQ
MAB_4370c|M.abscessus_ATCC_199 QRLYNLRTFGPTGIFEQDDGENWSEVQSISRGFVTNSVALNYQMGLDSER
:
Mb3186c|M.bovis_AF2122/97 VGAGSRFAERGHLSSKEWPGMLAFYRNLAMALVGDHPGAS----------
Rv3161c|M.tuberculosis_H37Rv VGAGSRFAERGHLSSKEWPGMLAFYRNLAMALVGDHPGAS----------
Mflv_2328|M.gilvum_PYR-GCK KGLRNDRLDHMS-LVKEEVVIAHYHSVVDRYMAASEVKA-----------
Mvan_4319|M.vanbaalenii_PYR-1 KGLRNDRLDHMS-LVKEEVVIAHYHSVVDRHLADSVTADTARSTSSVKES
MSMEG_5627|M.smegmatis_MC2_155 QGMKSMGFPGTKPNPYRERSTVNLHYQLSKYMGTGEPRDLQENA------
TH_1336|M.thermoresistible__bu QGMKSLGFPGPRPNPYRERSTVNLHHQLSKYMGTGEPREI----------
MMAR_0307|M.marinum_M QGMKSGGFGGPKPNPYMERSTANLHRNLAEYMGTGAPREL----------
MAV_5278|M.avium_104 RGMHQPGFTHLVLSSEERR-VINMHRNLERYLDLPAAQRMSGGAGT----
MUL_3820|M.ulcerans_Agy99 TNLES-GARQRLVFGRNEPGLQHRHITWAQALETPG--------------
MAB_4370c|M.abscessus_ATCC_199 EDGRHPGLTSELYSDAAGRSFYARWRDLMNTKAWHEEHV-----------
.
Mb3186c|M.bovis_AF2122/97 --
Rv3161c|M.tuberculosis_H37Rv --
Mflv_2328|M.gilvum_PYR-GCK --
Mvan_4319|M.vanbaalenii_PYR-1 RD
MSMEG_5627|M.smegmatis_MC2_155 --
TH_1336|M.thermoresistible__bu --
MMAR_0307|M.marinum_M --
MAV_5278|M.avium_104 --
MUL_3820|M.ulcerans_Agy99 --
MAB_4370c|M.abscessus_ATCC_199 --