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TLKMSTSGGSDTTARSLVDVDRGEISREIFTDASIFNRELEYLFPRSWLFVGHASQVSAPGQFFSSRMGS DPVLLTRDAQGGVNVLLNSCRHRGMAVCRYDEGRALQFTCPYHGWSYSMDGSLVSTPGDLHGVPQQGMAY GNGLDKAAWGLVRAAKVHNYKGLVFACWDPSAPEFDEYVGDFHHWLDNLSDAFDGTEGGTEVFRGVQKWR IKSNWKFVSENFLGDTYHGATTHASVEQVGIGPGGRNSRRHGERQDQGGFSKGRVKTSFRMGHGASDNLA YEIPYPEFAEEPALSEYFSQAWAVRKERLQAQGRQLGGRGPATMFPNMSFSAGFPRTILVSHPISPTETE VWRWYLIDKNAPDDVRDWLRRYYMRYSGPGGMTEQDDMENWNYATQASQGVIARRYPYNYQQGLDMETPS ALDQAVHSHHPIAGEVNARAFYRRWAEFTDNLSWPQLIELAKSDERAARS*
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. gilvum PYR-GCK | Mflv_0632 | - | - | 100% (471) | ring hydroxylating dioxygenase, alpha subunit |
| M. gilvum PYR-GCK | Mflv_0550 | - | 0.0 | 100.00% (471) | ring hydroxylating dioxygenase, alpha subunit |
| M. gilvum PYR-GCK | Mflv_0685 | - | 5e-69 | 35.38% (455) | ring hydroxylating dioxygenase, alpha subunit |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb3186c | - | 9e-18 | 28.90% (218) | dioxygenase |
| M. tuberculosis H37Rv | Rv3161c | - | 4e-18 | 29.36% (218) | dioxygenase |
| M. leprae Br4923 | - | - | - | - | - |
| M. abscessus ATCC 19977 | MAB_4370c | - | 2e-72 | 34.23% (444) | putative large terminal subunit of phenylpropionate dioxygenase |
| M. marinum M | MMAR_4422 | - | 5e-26 | 31.60% (212) | dioxygenase |
| M. avium 104 | MAV_3509 | - | 4e-62 | 33.26% (454) | phthalate dioxygenase large subunit |
| M. smegmatis MC2 155 | MSMEG_1908 | - | 7e-38 | 28.26% (368) | benzoate 1,2-dioxygenase alpha subunit |
| M. thermoresistible (build 8) | TH_2976 | - | 2e-32 | 28.06% (449) | PUTATIVE dioxygenase large subunit |
| M. ulcerans Agy99 | MUL_3820 | - | 1e-11 | 26.18% (233) | ring-hydroxylating dioxygenase, large terminal subunit |
| M. vanbaalenii PYR-1 | Mvan_0533 | - | 0.0 | 97.66% (471) | ring hydroxylating dioxygenase, alpha subunit |
CLUSTAL 2.0.9 multiple sequence alignment
Mb3186c|M.bovis_AF2122/97 ----------MLSTDNRAELGDILTDIGDYLDDNPPALSLPPAAYTSSEL
Rv3161c|M.tuberculosis_H37Rv ----------MLSTDNRAELGDILTDIGDYLDDNPPALSLPPAAYTSSEL
Mflv_0632|M.gilvum_PYR-GCK ------------MTLKMSTSGGSDTTARSLVDVDRGEISR--EIFTDASI
Mvan_0533|M.vanbaalenii_PYR-1 ------------MTLKMSTSGGSDTTARSLVDVDRGEISR--EIFTDASI
MAB_4370c|M.abscessus_ATCC_199 MASLPRPVAVAQGALCRPQALVRDIDVSGLVDSHGGLLDR--VIFTDGEL
MAV_3509|M.avium_104 ------------------MAGPDGGLLGVLENVRRGMIPA--HIYNDPEL
MSMEG_1908|M.smegmatis_MC2_155 -----------MTTHLSPDHLENVLADAVIEDPAAGVYRANRRIFTDDEI
TH_2976|M.thermoresistible__bu ---------------------------LIRPDRVHG------SLYTDPAI
MMAR_4422|M.marinum_M --------------------------MTAVRQPSALLPTLGGAYYTSAAV
MUL_3820|M.ulcerans_Agy99 ---------MDRDQLIDLTRRALKLARDKTTDLAPHQHAVDAREYTSLDR
: :..
Mb3186c|M.bovis_AF2122/97 WQLERERIFNRSWMLVAHVDQLAKTGDYVTVSVAGEPVMVVRDVDGQLHA
Rv3161c|M.tuberculosis_H37Rv WQLERERIFNRSWMLVAHVDQVAKTGDYVTVSVAGEPVMVVRDVDGQLHA
Mflv_0632|M.gilvum_PYR-GCK FNRELEYLFPRSWLFVGHASQVSAPGQFFSSRMGSDPVLLTRDAQGGVNV
Mvan_0533|M.vanbaalenii_PYR-1 FNRELEYLFPRSWLFVGHASQVSAPGQFFSSRMGSDPVLLTRDAQGGVNV
MAB_4370c|M.abscessus_ATCC_199 YRQELRRVFAPSWLFLAHADQFHRPGDFFTTYMGEDPVIVSMDKNRRIRA
MAV_3509|M.avium_104 FALEKRRLFARAWTFVGHESEIPHDGDYMVRRVLDDSFIITRGSDGRVRA
MSMEG_1908|M.smegmatis_MC2_155 FELEMKHIFEGNWVYLAHESQVPNPGDYFTTYIGRQPVVITRDRDGDLHC
TH_2976|M.thermoresistible__bu FAEELRKIWYRTWVFVGHESEVSQPNDYVRKKLGLQDVIMTRDRDGEIHL
MMAR_4422|M.marinum_M FAAEQQHVFESMWFCVVRAADLNEPGQFKAVQVGRESVLLVRGRDRRLRA
MUL_3820|M.ulcerans_Agy99 HERDRAMVLD-SPQLVGYSSELPGPDTYRTKTIMGRSILLTRAADRAVRA
: : : :. . : : .:: : :.
Mb3186c|M.bovis_AF2122/97 LSPICRHRLMLMVEPGAGRID-TLTCQYHLWRYGLDGRLRGAPHMAAN--
Rv3161c|M.tuberculosis_H37Rv LSPICRHRLMLMVEPGAGRID-TLTCQYHLWRYGLDGRLRGAPHMAAN--
Mflv_0632|M.gilvum_PYR-GCK LLNSCRHRGMAVCRYDEGRAL-QFTCPYHGWSYSMDGSLVSTPGDLHGVP
Mvan_0533|M.vanbaalenii_PYR-1 LLNSCRHRGMAVCRYDEGRAL-QFTCPYHGWSYSMDGSLVSTPGDLHGVP
MAB_4370c|M.abscessus_ATCC_199 FLNSCRHRGARVCRADYGVTR-GFTCTYHGWAYDLEGKLISVPNEEG---
MAV_3509|M.avium_104 LFNMCLHRGMQVCRAELGNAS-NFRCPYHGWTYRNDGRLTGLPFHREAYG
MSMEG_1908|M.smegmatis_MC2_155 LINACAHRGAMICRRKTDNRT-TLTCPFHGWTFRNDGTLLKVKDPDGAG-
TH_2976|M.thermoresistible__bu LLNRCSHRGNQVCADAKGNSS-TFRCPYHGWTFRNTGELVGFPFFKGYG-
MMAR_4422|M.marinum_M FLNICRHRGALLCTEPEGQVRRNLRCPYHSWTYGLDGTLIAAPNIAELTD
MUL_3820|M.ulcerans_Agy99 FDNVCLHRQSRVATGCGTALR--FTCPYHGWTYDNGGRLVGLPGREGFPG
: * ** : : * :* * : * *
Mb3186c|M.bovis_AF2122/97 -------LDFNRR-ECRLPQFA-VATWNGLVWINLDADA-EPIAAHL---
Rv3161c|M.tuberculosis_H37Rv -------LDFNRR-ECRLPQFA-VATWNGLVWINLDADA-EPIAAHL---
Mflv_0632|M.gilvum_PYR-GCK QQGMAYGNGLDKA-AWGLVRAAKVHNYKGLVFACWDPSA-PEFDEYVGDF
Mvan_0533|M.vanbaalenii_PYR-1 QQGMAYGNGLDKA-AWGLVRAAKVHNFKGLIFACWDPAA-PDFDEYVGDF
MAB_4370c|M.abscessus_ATCC_199 -----YPDSFSTD-DWGLVEVPHVDSYHGLIFGTWNPAP-VGLRESLGDM
MAV_3509|M.avium_104 ------GDDGFVK-DQTLLPAPNFAGYNGLLFISLDPAA-PPLQDFLGDF
MSMEG_1908|M.smegmatis_MC2_155 -----YPDTFNSNGSHNMTNVARFEGYRGFLFGSLNPDV-LPLTEHLGDT
TH_2976|M.thermoresistible__bu ----------TRKLDLPLGRVPRVDSYGGFVFGSFATEG-PTLREHLGAA
MMAR_4422|M.marinum_M ------TDGASIDRHRYGLVAVALREWLGYAWVCLAEDP-PSFEDDVVGS
MUL_3820|M.ulcerans_Agy99 -----------IAVGSDGLTELPATEFAGFLWVSLHPAARLDVPAHLG--
: * : . :
Mb3186c|M.bovis_AF2122/97 ----DLTDDEFAGYRLGEMVQVESWSHEWRANWKVAAEN-GHENYHVLGL
Rv3161c|M.tuberculosis_H37Rv ----DLTDDEFAGYRLGEMVQVESWSHEWRANWKVAAEN-GHENYHVLGL
Mflv_0632|M.gilvum_PYR-GCK HHWLDNLSDAFDGTEGGTEVFRGVQKWRIKSNWKFVSENFLGDTYHGATT
Mvan_0533|M.vanbaalenii_PYR-1 HYWLDNLADAFDGTEGATEVFRGVQKWRIKSNWKFVSENFLGDTYHGATT
MAB_4370c|M.abscessus_ATCC_199 AWYMDAMLDHDS---EGTVVVGGVHKWVLDGNWKLAAEQFATDWYHVNMS
MAV_3509|M.avium_104 R----FYLDYYTRQSAGGVELRGPQRWRIRANWKIGAENFAGDMYHTPHT
MSMEG_1908|M.smegmatis_MC2_155 TTVIDMLVDQSP---DGLEVLRGASTYTYDGNWKVQAEN-GADGYHVTAT
TH_2976|M.thermoresistible__bu AGEIDRLLRLSP--EGKVALDAGWLRHRTRANWKLLVEN-ETDGYHPQFV
MMAR_4422|M.marinum_M VTARLGDVSAIDTYRIEALQVGRRVSYDVAANWKLIVEN-FMECYHCATI
MUL_3820|M.ulcerans_Agy99 -----PLAGELQSWGIGRWSPLGEKVLGSPIDWKLATDT-FAENYHFATV
:**. : : **
Mb3186c|M.bovis_AF2122/97 HRQTLEPFVPGGGD------------------------------------
Rv3161c|M.tuberculosis_H37Rv HRQTLEPFVPGGGD------------------------------------
Mflv_0632|M.gilvum_PYR-GCK HASVEQVGIGPGGRNSRRHGERQDQGGFSKGRVKTSFRMGHGASDNLAYE
Mvan_0533|M.vanbaalenii_PYR-1 HASVEQVGIGPGGRNSRRHGERQDQGGFSKGRVKTSFRTGHGASDNLAYE
MAB_4370c|M.abscessus_ATCC_199 HASALMV-MAPQGKAPKDEIVHRTGRQYVD-------PNGHGAG-----F
MAV_3509|M.avium_104 HASIVDIGLFREPKAQKRKDGATYWAHRGGGTTYKLPPGGFEER------
MSMEG_1908|M.smegmatis_MC2_155 HWNYAATTSRRTCGESKNDTKALDAGSWGK--------SGGGYWSFPHGH
TH_2976|M.thermoresistible__bu HGGIFGVTGSTIGPLYSDSSTAVTRDLGGG--------HSENDLRPEFRK
MMAR_4422|M.marinum_M HPELTRVIPEFARGQAAQRSVGRGAEFG----------------------
MUL_3820|M.ulcerans_Agy99 HRRTFATIARSNCTVFDSYGPHHRLIFPLN--------------------
*
Mb3186c|M.bovis_AF2122/97 ---LDVRQYSRWALRLRVPFTVPVEAKSLQLNEVQKSN-LVVLWTF----
Rv3161c|M.tuberculosis_H37Rv ---LDVRQYSRWALRLRVPFTVPVEAKSLQLNEVQKSN-LVVLWTF----
Mflv_0632|M.gilvum_PYR-GCK IPYPEFAEEPALSEYFSQAWAVRKERLQAQGRQLGGRG-PATMFPN----
Mvan_0533|M.vanbaalenii_PYR-1 IAYPEFAEEPALSEYFSQAWALRKERLEAQGRQLGGRG-PATMFPN----
MAB_4370c|M.abscessus_ATCC_199 PVHPKNRFDARVVHQWTDYGALRERLGEARVQGPLTNG-HATVFPN----
MAV_3509|M.avium_104 --MRYVGYPDEMIGRIKQVWTPRQQ--RVVGEDGFMVS-AATCFPN----
MSMEG_1908|M.smegmatis_MC2_155 LCLWTWAANPQDRPLWDRLDELKARFGDAKGEFMVKGSRNLCLYPN----
TH_2976|M.thermoresistible__bu FAEPLRWFGTTESRVPDYVAAMRARYGAEADQILIEGSPHVMIFPN----
MMAR_4422|M.marinum_M --SAVAGFTVDGRAGFTALPGIRPEQDRRYFAITVKPTVFINLVPDHAII
MUL_3820|M.ulcerans_Agy99 TILELENVPEGQWDPFHHMVVIYALFPNVVLSVTIANGELFRIYPG----
.
Mb3186c|M.bovis_AF2122/97 --PNSALAIAGER-------------VVWFGFIP--------QSIDR---
Rv3161c|M.tuberculosis_H37Rv --PNSALAIAGER-------------VVWFGFIP--------QSIDR---
Mflv_0632|M.gilvum_PYR-GCK --MSFSAGFPRTI-------------LVSHPISPTETEVWRWYLIDKNAP
Mvan_0533|M.vanbaalenii_PYR-1 --MSFSAGFPRTI-------------LVSHPISPTETEVWRWYLIDKNAP
MAB_4370c|M.abscessus_ATCC_199 --LSYLP-VNGSV-------------RIWHPKGPDKMEVWAWTIVDRSMP
MAV_3509|M.avium_104 --LSFVHNWPKVRDGRDDETLPFISIRLWQPISENETEVCSWFAVDSAAP
MSMEG_1908|M.smegmatis_MC2_155 --VYVMDQFSTQIR-------------HFRPIAPDKTEVTIYCIAPKGES
TH_2976|M.thermoresistible__bu --LFIAEIQVFNI----------------QPVAVNECVQYS-TAVQLVGA
MMAR_4422|M.marinum_M HRM-----------------------------------------------
MUL_3820|M.ulcerans_Agy99 --------------------------------------------------
Mb3186c|M.bovis_AF2122/97 -----------VQVLG--GVLTTPELAADAAATAQTSQFVMAMINDEDRL
Rv3161c|M.tuberculosis_H37Rv -----------VQVLG--GVLTTPELAADAAATAQTSQFVMAMINDEDRL
Mflv_0632|M.gilvum_PYR-GCK DDVRDWLRRYYMRYSGPGGMTEQDDMENWNYATQASQGVIARRYPYNYQQ
Mvan_0533|M.vanbaalenii_PYR-1 DDVRDWLRRYYMRYSGPGGMTEQDDMENWNYATQASQGVIARRYPYNYQQ
MAB_4370c|M.abscessus_ATCC_199 EEVRDAQRLYNLRTFGPTGIFEQDDGENWSEVQSISRGFVTNSVALNYQM
MAV_3509|M.avium_104 AHYKQDSYKAYLMCFGSTGMFEQDDVENWVSLTTTAGGSMARRLLLNSRM
MSMEG_1908|M.smegmatis_MC2_155 AAARAHRIRQYEDFFNASGMATPDDLEEFRSCQLTFR---AQAAPWNDMS
TH_2976|M.thermoresistible__bu PELNRRMVSQCIGSVGPAGMLLADDTEMYERNQQGLEALTPEWLDIRRGL
MMAR_4422|M.marinum_M -----------FPIAADRTIVECDWLYAPEVAAAGDIAHSVELFHRVNEQ
MUL_3820|M.ulcerans_Agy99 -------DRPGSSITVHQNSTPLDLSDESVATGAQAVFDYAHATVRDEDY
Mb3186c|M.bovis_AF2122/97 GLEAVQVGAGSRFAERGHLSSKEWPGMLAFYRNLAMALVGDHPGAS----
Rv3161c|M.tuberculosis_H37Rv GLEAVQVGAGSRFAERGHLSSKEWPGMLAFYRNLAMALVGDHPGAS----
Mflv_0632|M.gilvum_PYR-GCK GLDMETPSALDQAVHSHHPIAGEVNARAFYRRWAEFTDNLSWPQLIELAK
Mvan_0533|M.vanbaalenii_PYR-1 GLDMETPSALDQAVHSHHPIAGEVNARAFYRRWAEFTDNLSWPQLIELAK
MAB_4370c|M.abscessus_ATCC_199 GLDSEREDGRHPGLTS--ELYSDAAGRSFYARWRDLMNTKAWHEEHV---
MAV_3509|M.avium_104 GLYDDGRPVVEALAPS--AFHGPGRAQVGYNEHNQRALLAMWADYLQEGD
MSMEG_1908|M.smegmatis_MC2_155 RGAQHWLSGPDPVAESLGMHGIVSSGLRNEDEGLYPVQHGYWLETLRRAV
TH_2976|M.thermoresistible__bu NREHVDDHGFTVGGPT-----DETGMRAFWSEYRRLMEAE----------
MMAR_4422|M.marinum_M DFDACQRTQPAMSSRAYRTGGVLVPAEHHIAEFHQWVIARIGTPAVTG--
MUL_3820|M.ulcerans_Agy99 RLAETLQTNLESGARQRLVFGRNEPGLQHRHITWAQALETPG--------
Mb3186c|M.bovis_AF2122/97 --------
Rv3161c|M.tuberculosis_H37Rv --------
Mflv_0632|M.gilvum_PYR-GCK SDERAARS
Mvan_0533|M.vanbaalenii_PYR-1 SDERAARS
MAB_4370c|M.abscessus_ATCC_199 --------
MAV_3509|M.avium_104 ACENR---
MSMEG_1908|M.smegmatis_MC2_155 TSNPTEH-
TH_2976|M.thermoresistible__bu --------
MMAR_4422|M.marinum_M --------
MUL_3820|M.ulcerans_Agy99 --------