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TGTLVEALRDAARDTPDRVLVRDDAVALTCAELLSAATGLAHALTRRMPAGSVVSFTVPNWHEAAIIYLG ATLAGMVVNPILPSLRERELSFILDDADSRMIFVPESFGGHDYAAMLDRVAPGLPSPPEVVVVRGEGRVT LADLLAEPADGVLPAPRPTDTRMILYTSGTTGRPKGVLHTHESLAALIAQLGRFWRIDRGDTFLVPSPIA HIGGSIYAFECPLLLGTEAVLMQRWDPDAAVGLMLEHRVTHMAGATPFLVGLLAAAQRAGTRLPDLKVFI CGGASVPPTLIRSAAAYFDKAAVSRVYGSTEVPVTTVGALDDIDRAADTDGRPGIAEVRIVDGEIRARGP QMFTGYLHPEDDRESFDDAGYFRTGDLGRFTEDNHLVVTGRAKDIIIRNGENISPKEVEDILVTHPRIAE IAVVGIPDPRTGERACAALVTTEDPPPDIAELKDFLTDRGVARFKVPEQIHVVGALPKNDAGKVLKHVLK AELTKES*
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. gilvum PYR-GCK | Mflv_1784 | - | - | 100% (498) | AMP-dependent synthetase and ligase |
| M. gilvum PYR-GCK | Mflv_2523 | - | 9e-61 | 34.44% (482) | AMP-dependent synthetase and ligase |
| M. gilvum PYR-GCK | Mflv_0027 | - | 1e-48 | 30.77% (507) | AMP-dependent synthetase and ligase |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb2533c | fadD35 | 8e-52 | 31.33% (482) | AMP-binding domain protein |
| M. tuberculosis H37Rv | Rv2505c | fadD35 | 8e-52 | 31.33% (482) | AMP-binding domain protein |
| M. leprae Br4923 | MLBr_01051 | xclC | 1e-40 | 29.18% (497) | acyl-CoA synthetase |
| M. abscessus ATCC 19977 | MAB_2247c | - | 1e-51 | 32.24% (518) | bifunctional salicyl-AMP ligase/salicyl-S-arcp synthetase |
| M. marinum M | MMAR_0303 | - | 0.0 | 65.20% (500) | acyl-CoA synthetase |
| M. avium 104 | MAV_5212 | - | 0.0 | 64.73% (516) | AMP-binding enzyme, putative |
| M. smegmatis MC2 155 | MSMEG_5631 | - | 0.0 | 68.54% (499) | AMP-dependent synthetase and ligase |
| M. thermoresistible (build 8) | TH_3067 | - | 0.0 | 71.12% (509) | PUTATIVE AMP-binding enzyme, putative |
| M. ulcerans Agy99 | MUL_3641 | mbtA | 7e-50 | 31.04% (509) | bifunctional enzyme MbtA: salicyl-AMP ligase (SAL-AMP |
| M. vanbaalenii PYR-1 | Mvan_4966 | - | 0.0 | 80.61% (495) | AMP-dependent synthetase and ligase |
CLUSTAL 2.0.9 multiple sequence alignment
Mb2533c|M.bovis_AF2122/97 ---------------MAAAEVVDPNRLSYDRGPSAPSLLESTIGANLAAT
Rv2505c|M.tuberculosis_H37Rv ---------------MAAAEVVDPNRLSYDRGPSAPSLLESTIGANLAAT
Mflv_1784|M.gilvum_PYR-GCK --------------------------------------MTGTLVEALRDA
Mvan_4966|M.vanbaalenii_PYR-1 --------------------------------------MT-TLADALRDA
TH_3067|M.thermoresistible__bu ---------MTETPGAGYRTDRPGHAERAAQAYARGLWVPTTLAEALAEA
MSMEG_5631|M.smegmatis_MC2_155 --------------------------MRAEDAYERGLWVRTTLADTLAEA
MAV_5212|M.avium_104 -----------------MTARAAPDTAQTSLAYRRGWWVRSTLADAARAA
MMAR_0303|M.marinum_M -----MGGPKVPQRRLIDGRWVRWDADRAAGAYAHRWWVRRTLADALREA
MAB_2247c|M.abscessus_ATCC_199 MTQPRSTTTASSAATPALDGFVPFPSVRADEYRRAGYWTGNTVESLLRVS
MUL_3641|M.ulcerans_Agy99 -----------------MAGFIPYPPERVASYRAAGYWTGRSIGSLLRQA
MLBr_01051|M.leprae_Br4923 -----------------------------------------------MLL
Mb2533c|M.bovis_AF2122/97 AARYGHREALVDMVARRRFNYSELLTDVHRLATGLVRAGIGPGDRVGIWA
Rv2505c|M.tuberculosis_H37Rv AARYGHREALVDMVARRRFNYSELLTDVHRLATGLVRAGIGPGDRVGIWA
Mflv_1784|M.gilvum_PYR-GCK ARDTPDRVLVRDDAVA--LTCAELLSAATGLAHALTRR-MPAGSVVSFTV
Mvan_4966|M.vanbaalenii_PYR-1 ARDTPDRILLRDDEAS--LSSAELLSAATNLAHALVRR-MPVGSVVSFAV
TH_3067|M.thermoresistible__bu ARTTPDRTVLVDGAVR--LDCRSLHARATALAGALLRR-IPAGAVVSFML
MSMEG_5631|M.smegmatis_MC2_155 ARDTPQRVLLIDGDVR--LTCAELHSRAKALAHEMAAR-MPAGSVVSFML
MAV_5212|M.avium_104 AQRTPERIALVDNEVR--LNCATLYAQAGELAAALLAR-IPAGSVVSFML
MMAR_0303|M.marinum_M AQSTPQRVALVDADRQ--LDCQELHRQAMALAQHLRSR-MPAGSVVSFML
MAB_2247c|M.abscessus_ATCC_199 AARRPCHPAVIDEHRA--LSYRELDEAADNAAHAFLRLGIAPGDRVLLQL
MUL_3641|M.ulcerans_Agy99 AARWPDRVGVVDAGAS--HTFCELDKLADRVAAGFVSLGIAPGDRVLLQL
MLBr_01051|M.leprae_Br4923 ASLNPSVVTATDIADAVCVDGVVLSRSGLVGAATSVAERVGGARLVAVLA
* * * * : . * .
Mb2533c|M.bovis_AF2122/97 PNRWEWVLVQYATAEIGAILVTINPAYRVREVEYALRQSGVAMVIAVASF
Rv2505c|M.tuberculosis_H37Rv PNRWEWVLVQYATAEIGAILVTINPAYRVREVEYALRQSGVAMVIAVASF
Mflv_1784|M.gilvum_PYR-GCK PNWHEAAIIYLGATLAGMVVNPILPSLRERELSFILDDADSRMIFVPESF
Mvan_4966|M.vanbaalenii_PYR-1 PNWHEAAVIYLGATLAGMVVNPILPSLRERELSFILADAGSRAIFIPQTF
TH_3067|M.thermoresistible__bu PNWHEAAVIYLGATLAGMVVNPILPSLRDRELRFILDDADSRMIFIPETF
MSMEG_5631|M.smegmatis_MC2_155 PNWHEAAIVYLASTLAGMVINPILPSLRDHELRFILDDAASRMIFIPGEF
MAV_5212|M.avium_104 PNWHEAAVIYLAATLAGMVVNPILPSLRDHDLRFVLEDAGAAMVFVPHRY
MMAR_0303|M.marinum_M PNWHEAAVIYLASTLAAMVANPVLPSLREHELRFILEDAGSRMIFAPADF
MAB_2247c|M.abscessus_ATCC_199 PNRASFAVALFGLMRAGAIPVMCLPGHRAAELSHFIEVADAVGLVIPDSA
MUL_3641|M.ulcerans_Agy99 PNSCRFAVALFGLLRAGAIPVMCLPGHRLAELHHFAEVSGAVGLIVAQHA
MLBr_01051|M.leprae_Br4923 TPTASTVLAITGCLIAGVPVVPVPADIGVVERRHMLTDSGAQAWLGPGPS
. .: . . . : . : .
Mb2533c|M.bovis_AF2122/97 KDADYAAMLAEVGPRCPDLADVILLESDRWDALAGAEPDLPALQQTAAR-
Rv2505c|M.tuberculosis_H37Rv KDADYAAMLAEVGPRCPDLADVILLESDRWDALAGAEPDLPALQQTAAR-
Mflv_1784|M.gilvum_PYR-GCK GGHDYAAMLDRVAPGLPSPPEVVVVRGE------GRVTLADLLAEPADG-
Mvan_4966|M.vanbaalenii_PYR-1 GGHDYAAMLDRVVAGLPSPPEVVVVRGEP-----RRPALTDLVSEPRDG-
TH_3067|M.thermoresistible__bu GNHDYAAMLDRVTAELPNPPQVVVVRGAVR---PDRVSYDALLSGPVDRP
MSMEG_5631|M.smegmatis_MC2_155 GQHDYAAMLQRVCAHLPTPPQVVVVRGEP-----GPHTAFTALGAPGGD-
MAV_5212|M.avium_104 GGHDYPAMLDRVTAAMSAAPQVVVLRGPDTGRTGRHTPYRCMLGGPPGG-
MMAR_0303|M.marinum_M RGHDYTSMLRRVAAQLPSRPEVIEVRGQCP---DGVSTFQSLLVGRETAA
MAB_2247c|M.abscessus_ATCC_199 GGFDYRALAVALSQRHPRLRTVVVEGDAGP-----FASWTSLLKSVEPQG
MUL_3641|M.ulcerans_Agy99 GGFDYRSMAERLVAAHPQLRHVIIDGESGP-----FLPWSSLSEGATPD-
MLBr_01051|M.leprae_Br4923 --------ADSAPDGLPHIPVQLDARSWNR--------------------
. :
Mb2533c|M.bovis_AF2122/97 ----LDGSDPVNIQYTSGTTAYPKGVTLSHRNILNNGYLVGELLGYTAQD
Rv2505c|M.tuberculosis_H37Rv ----LDGSDPVNIQYTSGTTAYPKGVTLSHRNILNNGYLVGELLGYTAQD
Mflv_1784|M.gilvum_PYR-GCK VLPAPRPTDTRMILYTSGTTGRPKGVLHTHESLAALIAQLGRFWRIDRGD
Mvan_4966|M.vanbaalenii_PYR-1 TLPDLDPAATRMILYTSGTTGRPKGVLHSHKSLGALIAQLGRYWRIDPGD
TH_3067|M.thermoresistible__bu VLPALDPDAVRMILYTSGTTGRPKGVLHSHNSIHALIHQLREHWLIDPGD
MSMEG_5631|M.smegmatis_MC2_155 -LPALDPDSVRMMMHTSGTTGRPKGVLHSHNSIHALICQLRENWMIDPGD
MAV_5212|M.avium_104 -LPALDPDAVRMILYTSGTTSRPKGVLHTHNSIHALICQIRDHWAIDPGD
MMAR_0303|M.marinum_M PLPTADPDAVRMVLYTSGTTGRPKGVLHTHNSIHALICQIRDHWMVEPGD
MAB_2247c|M.abscessus_ATCC_199 PLPTVQTDTPALLLVSGGTTAAPKLIPRTHEDYVYNATQAARVCGLTQDD
MUL_3641|M.ulcerans_Agy99 --IDVDTTSPALLLVSGGTTGTPKLIPRTHDDYVYNATASAQLCRLTADD
MLBr_01051|M.leprae_Br4923 -YPEPSPDATAMVIYTSGTTGPPKGVLLSRRAIAVDLDALAQAWQWTADD
: :.***. ** : :: *
Mb2533c|M.bovis_AF2122/97 RICIPVPFYHCFGMVMGNLAAT----SHGAAMVIPAPGFDPAATLRAVQD
Rv2505c|M.tuberculosis_H37Rv RICIPVPFYHCFGMVMGNLAAT----SHGAAMVIPAPGFDPAATLRAVQD
Mflv_1784|M.gilvum_PYR-GCK TFLVPSPIAHIGGSIYAFECP-----LLLGTEAVLMQRWDPDAAVGLMLE
Mvan_4966|M.vanbaalenii_PYR-1 TFLVPSPIAHIGGSIYAFECP-----LLLGTQAVLMQRWDPDAAVALMLR
TH_3067|M.thermoresistible__bu TFLVPSPIAHIGGSIYAFECP-----LLAGTTAVLMDRWRPDEAVRLMRA
MSMEG_5631|M.smegmatis_MC2_155 TFLVPSPIAHIGGSIYAFECP-----LLLGTTAVLMQRWNADDGVALMSE
MAV_5212|M.avium_104 TFLVPSPLAHIGGSIYAFECP-----LLLGTTAVLMDRWDPARAVALMTA
MMAR_0303|M.marinum_M GFLVASPIAHIGGSIYAFECP-----LLLGTTAVLLDRWDADRAVQLMTS
MAB_2247c|M.abscessus_ATCC_199 VYLVALPAGHN----FPLACPGLLGAISTGATTVFLSDPSPESAFATIAR
MUL_3641|M.ulcerans_Agy99 VYLVSLPAAHN----FPLACPGILGSMAVGATIVFSTDPSPEAAFTLIER
MLBr_01051|M.leprae_Br4923 VLVHGLPLFHVHGLVLGLLGS-----LRIGNRFVHTGKPTPTAYAQACSE
* * . . : .
Mb2533c|M.bovis_AF2122/97 ERCTSLYGVPTMFIAELGLPDFTDYELGSLRTGIMAGAACPVEVMRKVIS
Rv2505c|M.tuberculosis_H37Rv ERCTSLYGVPTMFIAELGLPDFTDYELGSLRTGIMAGAACPVEVMRKVIS
Mflv_1784|M.gilvum_PYR-GCK HRVTHMAGATPFLVGLLAAAQRAGTRLPDLKVFICGGASVPPTLIRSAAA
Mvan_4966|M.vanbaalenii_PYR-1 HRCTHMAGATPFLDGLLAAAERAGTRLPDLKVFICGGASVPPSLIRRAAD
TH_3067|M.thermoresistible__bu EHCTHMAGATPFLDGLLTAARQAGTRLPDLKVFICGGASVSPSLIRSAAD
MSMEG_5631|M.smegmatis_MC2_155 HHCTHMAGATPFLDQLLNAAERAGTRLPDLKVFICGGASVPPSLIRRAST
MAV_5212|M.avium_104 HRCTHMAGATPFLQQLLSAAANAGTRLPDLKVFICGGASVSPSLIRRAAA
MMAR_0303|M.marinum_M KRCTHMAGATPFLEQLLAAAQRAGTRLPDLKFFVCGGASVSPSLIRCATD
MAB_2247c|M.abscessus_ATCC_199 HRVSVTAVVPSLAQLWAQATAWEPVLPETLRLLQVGGAKLGADDARAVRE
MUL_3641|M.ulcerans_Agy99 HGVTVTALVPALAKLWTQACDWEPVIPKSLRLLQVGGSRLEPEDARQARE
MLBr_01051|M.leprae_Br4923 AGGSLYFGVPTVWSRLVADEAVARALRPAR--LLVSGSASLPVPVFDRLA
: .... .*:
Mb2533c|M.bovis_AF2122/97 RMHMPGVSICYGMTETSPVSTQTRADDSVDRRVGTVGRVGPHLEIKVVDP
Rv2505c|M.tuberculosis_H37Rv RMHMPGVSICYGMTETSPVSTQTRADDSVDRRVGTVGRVGPHLEIKVVDP
Mflv_1784|M.gilvum_PYR-GCK YFDKAAVSRVYGSTEVPVTTVGALD--DIDRAADTDGRPG-IAEVRIVD-
Mvan_4966|M.vanbaalenii_PYR-1 YFDKAAVSRVYGSTEVPVTTVGSLD--DVDRAADTDGRPG-IADVTLVD-
TH_3067|M.thermoresistible__bu YFERAVVTRVYGSTEVPVTTVGVLGPADADRAAETDGRPG-IAEVRLADH
MSMEG_5631|M.smegmatis_MC2_155 CFEKAVVSRVYGSTEVPVTTVGSIEPGDVEHAACTDGRPG-LAEIRLVD-
MAV_5212|M.avium_104 YFDRAVVTRVYGCTEVPVATVGAPRPQEADYAADTDGRPG-IAEIKLVAH
MMAR_0303|M.marinum_M YFDKAVVTRVYGSTEVPVTTIGAPD--SSAHAAATDGRVG-AADVKLVA-
MAB_2247c|M.abscessus_ATCC_199 SLTQG-LQQVFGMAEGLLCLTQPGDPTDILDNSQGRPMCD-ADEVRIVDE
MUL_3641|M.ulcerans_Agy99 ALTPG-LQQVFGMAEGLLNFTRLEDPPDLLEHTQGRPLCS-ADELRIVDG
MLBr_01051|M.leprae_Br4923 HLTGHRPIERYGSTESLITLSTLAD---GERRAGWVGLPLAGVQTRLVDE
: :* :* : :.
Mb2533c|M.bovis_AF2122/97 ATGETVPR-GVVGEFCTRGYSVMAGYWNDPQKTAEVIDADGWMHTGDLAE
Rv2505c|M.tuberculosis_H37Rv ATGETVPR-GVVGEFCTRGYSVMAGYWNDPQKTAEVIDADGWMHTGDLAE
Mflv_1784|M.gilvum_PYR-GCK ------------GEIRARGPQMFTGYLHP-EDDRESFDDAGYFRTGDLGR
Mvan_4966|M.vanbaalenii_PYR-1 ------------GEIRARGPQMLTGYLHP-EDNSESFDAEGFFRTGDLGH
TH_3067|M.thermoresistible__bu AAAPPGA-----GEVLARGPQMLVGYLHP-EDERDAFDADGFFRTGDLAR
MSMEG_5631|M.smegmatis_MC2_155 ------------GEIRARGPQMLLGYLHA-EDN--AFDEQGFFRTGDLGR
MAV_5212|M.avium_104 PAAPTGD-----GEICVRGPQMLRGYRHP-EDDAESFDAAGFFRTGDLGR
MMAR_0303|M.marinum_M ------------GEIRVRGPQMLVGYRHP-EDEAGSFDAQGYFRTGDLGH
MAB_2247c|M.abscessus_ATCC_199 DGADVPAG--ETGELLVRGPYTLNGYYRADADNMRSFTVDGFYRSGDRVR
MUL_3641|M.ulcerans_Agy99 AGAPVAPG--AEGELLVRGPYTINGYFCADRDNERCFDPAGFYRSGDLVR
MLBr_01051|M.leprae_Br4923 SGGPVPYDGETVGRLQVRSPTMFGGYLNRPEATAEAFDEDGWYRTGDVAV
*.. .*. : ** : *: ::**
Mb2533c|M.bovis_AF2122/97 MDPSG----YVRIAGRIKDLVVRGGENISPREIEELLHTHPDIVDGHVIG
Rv2505c|M.tuberculosis_H37Rv MDPSG----YVRIAGRIKDLVVRGGENISPREIEELLHTHPDIVDGHVIG
Mflv_1784|M.gilvum_PYR-GCK FTEDN----HLVVTGRAKDIIIRNGENISPKEVEDILVTHPRIAEIAVVG
Mvan_4966|M.vanbaalenii_PYR-1 LTDDG----YLVVTGRAKDLIIRNGENISPKEVEDILVTHPQIAEIAIVG
TH_3067|M.thermoresistible__bu WVDGD----HLVITGRAKDIIIRNGENISPKEVEDLLIGHPGVAEIAVVG
MSMEG_5631|M.smegmatis_MC2_155 WVDGD----YLTVTGRAKDLIIRNGENISPKEIEDILVGELGINEIAVVG
MAV_5212|M.avium_104 WALADSADRYLVVTGRAKDVIIRSGENISAKEVEDLLADHPGIAEIAVVG
MMAR_0303|M.marinum_M WVDDE----YLVVTGRAKDIIIRNGENISPKEIEDILITHPGIADVAIVG
MAB_2247c|M.abscessus_ATCC_199 ALPGG----YLEVTGRIKDVILRGGESIAALDLESHLQTHPAVYAAAAVG
MUL_3641|M.ulcerans_Agy99 LRDDG----YLQVTGRVKDVICRCGETISAQDLEEQLLSHPAIRSAAAVP
MLBr_01051|M.leprae_Br4923 VDSGG---MHRIVGRESVDLIKLGGYRIGAGEIEMALLGHPDVREVAVVG
: : . *:: * *.. ::* * . : :
Mb2533c|M.bovis_AF2122/97 VPDAKYGEELMAVVKLRN-DAPELTIERLREYCMGR-IARFKIPRYLWIV
Rv2505c|M.tuberculosis_H37Rv VPDAKYGEELMAVVKLRN-DAPELTIERLREYCMGR-IARFKIPRYLWIV
Mflv_1784|M.gilvum_PYR-GCK IPDPRTGERACAALVTTE--DPPPDIAELKDFLTDRGVARFKVPEQIHVV
Mvan_4966|M.vanbaalenii_PYR-1 IPDLRTGERACAVIVAAD--SAAPDVAEIRDFLVARGVAKFKAPERVVVV
TH_3067|M.thermoresistible__bu VPDPRTGERACAAIVPTG--RPGPDVAELREFLLARGVAKFKAPEQVVFF
MSMEG_5631|M.smegmatis_MC2_155 LPDERTGERACAVVVTED--GARPDVSALAEVLTKAGVAKFKIPEQVEIW
MAV_5212|M.avium_104 LPDERTGERACAVIVPTP--GASPDVASLLALLVSKGVAKFKAPEQVVLW
MMAR_0303|M.marinum_M LPDDRTGERACAVIVAAA--DPPPGLPGVRAFLEGAGVARFKIPEQVVIW
MAB_2247c|M.abscessus_ATCC_199 LPDQYLGEIVCAAIVFKG---KPVAAAELNQHLQARGAATHSRVDKLVAV
MUL_3641|M.ulcerans_Agy99 LPDPDMGELICAAIVFAGPPQTPQTLSGLNDYLEQRGVAAHIRIDRLVAM
MLBr_01051|M.leprae_Br4923 LPDEDLGQRIVAFVVGAE----ALDADELINYVAQQ-LSIHKRPREVRFV
:** *: * : : : . :
Mb2533c|M.bovis_AF2122/97 DEFPMTVTGKVRKVEMRQQALEYLRGQQ-
Rv2505c|M.tuberculosis_H37Rv DEFPMTVTGKVRKVEMRQQALEYLRGQQ-
Mflv_1784|M.gilvum_PYR-GCK GALPKNDAGKVLKHVLKAELTKES-----
Mvan_4966|M.vanbaalenii_PYR-1 DALPKNDAGKVLKHAIRAVLLEKPREGEP
TH_3067|M.thermoresistible__bu DALPKNDAGKVLKHQIQATLTAAEVSG--
MSMEG_5631|M.smegmatis_MC2_155 DALPKNDAGKVLKHQIRTQLSKVE-----
MAV_5212|M.avium_104 DALPKNDAGKVLKHRIRAALSKDG-----
MMAR_0303|M.marinum_M DHLPKNHAGKIVKHRIKAALMKAEQ----
MAB_2247c|M.abscessus_ATCC_199 SALPLTAVGKIDKRALLASLTG-------
MUL_3641|M.ulcerans_Agy99 PSLPTTPVGKIDKKAIASQILTTTAT---
MLBr_01051|M.leprae_Br4923 DALPRNAMGKVLKKQLLFEG---------
:* . **: * :