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GGPKVPQRRLIDGRWVRWDADRAAGAYAHRWWVRRTLADALREAAQSTPQRVALVDADRQLDCQELHRQA MALAQHLRSRMPAGSVVSFMLPNWHEAAVIYLASTLAAMVANPVLPSLREHELRFILEDAGSRMIFAPAD FRGHDYTSMLRRVAAQLPSRPEVIEVRGQCPDGVSTFQSLLVGRETAAPLPTADPDAVRMVLYTSGTTGR PKGVLHTHNSIHALICQIRDHWMVEPGDGFLVASPIAHIGGSIYAFECPLLLGTTAVLLDRWDADRAVQL MTSKRCTHMAGATPFLEQLLAAAQRAGTRLPDLKFFVCGGASVSPSLIRCATDYFDKAVVTRVYGSTEVP VTTIGAPDSSAHAAATDGRVGAADVKLVAGEIRVRGPQMLVGYRHPEDEAGSFDAQGYFRTGDLGHWVDD EYLVVTGRAKDIIIRNGENISPKEIEDILITHPGIADVAIVGLPDDRTGERACAVIVAAADPPPGLPGVR AFLEGAGVARFKIPEQVVIWDHLPKNHAGKIVKHRIKAALMKAEQ*
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. marinum M | MMAR_0303 | - | - | 100% (536) | acyl-CoA synthetase |
| M. marinum M | MMAR_1606 | - | 6e-51 | 30.30% (505) | fatty-acid-CoA ligase |
| M. marinum M | MMAR_3852 | fadD35 | 6e-49 | 30.47% (512) | fatty-acid-CoA ligase FadD35 |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb3116 | fadD13 | 3e-47 | 29.75% (511) | chain-fatty-acid-CoA ligase |
| M. gilvum PYR-GCK | Mflv_1784 | - | 0.0 | 65.20% (500) | AMP-dependent synthetase and ligase |
| M. tuberculosis H37Rv | Rv3089 | fadD13 | 3e-47 | 29.75% (511) | chain-fatty-acid-CoA ligase |
| M. leprae Br4923 | MLBr_02546 | fadD2 | 4e-36 | 26.82% (522) | acyl-CoA synthetase |
| M. abscessus ATCC 19977 | MAB_4377c | - | 5e-48 | 27.83% (521) | AMP-binding domain protein |
| M. avium 104 | MAV_5212 | - | 0.0 | 66.42% (533) | AMP-binding enzyme, putative |
| M. smegmatis MC2 155 | MSMEG_5631 | - | 0.0 | 66.99% (515) | AMP-dependent synthetase and ligase |
| M. thermoresistible (build 8) | TH_3067 | - | 0.0 | 66.54% (526) | PUTATIVE AMP-binding enzyme, putative |
| M. ulcerans Agy99 | MUL_3641 | mbtA | 3e-44 | 29.91% (545) | bifunctional enzyme MbtA: salicyl-AMP ligase (SAL-AMP |
| M. vanbaalenii PYR-1 | Mvan_4966 | - | 0.0 | 67.61% (497) | AMP-dependent synthetase and ligase |
CLUSTAL 2.0.9 multiple sequence alignment
Mflv_1784|M.gilvum_PYR-GCK -------------------------------------------MTGTLVE
Mvan_4966|M.vanbaalenii_PYR-1 -------------------------------------------MT-TLAD
TH_3067|M.thermoresistible__bu --------------MTETPGAGYRTDRPGHAERAAQAYARGLWVPTTLAE
MSMEG_5631|M.smegmatis_MC2_155 -------------------------------MRAEDAYERGLWVRTTLAD
MAV_5212|M.avium_104 ----------------------MTARAAPDTAQTSLAYRRGWWVRSTLAD
MMAR_0303|M.marinum_M ----------MGGPKVPQRRLIDGRWVRWDADRAAGAYAHRWWVRRTLAD
Mb3116|M.bovis_AF2122/97 --------------------------------------------MKNIGW
Rv3089|M.tuberculosis_H37Rv --------------------------------------------MKNIGW
MAB_4377c|M.abscessus_ATCC_199 ------------------------------MKAYDAGATLSPIIEETIGE
MUL_3641|M.ulcerans_Agy99 ----------------------MAGFIPYPPERVASYRAAGYWTGRSIGS
MLBr_02546|M.leprae_Br4923 MTKLRHYLERGAAEVHYLRSIFESGALGFEPPLTYAAMAVAMRKWGDLAM
:
Mflv_1784|M.gilvum_PYR-GCK ALRDAARDTPDRVLVRDD--AVALTCAELLSAATGLAHALTRR-MPAGSV
Mvan_4966|M.vanbaalenii_PYR-1 ALRDAARDTPDRILLRDD--EASLSSAELLSAATNLAHALVRR-MPVGSV
TH_3067|M.thermoresistible__bu ALAEAARTTPDRTVLVDG--AVRLDCRSLHARATALAGALLRR-IPAGAV
MSMEG_5631|M.smegmatis_MC2_155 TLAEAARDTPQRVLLIDG--DVRLTCAELHSRAKALAHEMAAR-MPAGSV
MAV_5212|M.avium_104 AARAAAQRTPERIALVDN--EVRLNCATLYAQAGELAAALLAR-IPAGSV
MMAR_0303|M.marinum_M ALREAAQSTPQRVALVDA--DRQLDCQELHRQAMALAQHLRSR-MPAGSV
Mb3116|M.bovis_AF2122/97 MLRQRATVSPRLQAYVEPSTDVRMTYAQMNALANRCADVLTALGIAKGDR
Rv3089|M.tuberculosis_H37Rv MLRQRATVSPRLQAYVEPSTDVRMTYAQMNALANRCADVLTALGIAKGDR
MAB_4377c|M.abscessus_ATCC_199 NFERIARAHPDVGALVDVSGDRRWTYRELDAEINRVARGLMSLGVAAGDR
MUL_3641|M.ulcerans_Agy99 LLRQAAARWPDRVGVVDAG--ASHTFCELDKLADRVAAGFVSLGIAPGDR
MLBr_02546|M.leprae_Br4923 LPAMPARRSPHRAALIDE--EGTLTYKELDRAAHALANGLIAKGVRGGDG
* * : : * : : *
Mflv_1784|M.gilvum_PYR-GCK VSFTVPNWHEAAIIYLGATLAGMVVNPILPSLRERELSFILDDADSRMIF
Mvan_4966|M.vanbaalenii_PYR-1 VSFAVPNWHEAAVIYLGATLAGMVVNPILPSLRERELSFILADAGSRAIF
TH_3067|M.thermoresistible__bu VSFMLPNWHEAAVIYLGATLAGMVVNPILPSLRDRELRFILDDADSRMIF
MSMEG_5631|M.smegmatis_MC2_155 VSFMLPNWHEAAIVYLASTLAGMVINPILPSLRDHELRFILDDAASRMIF
MAV_5212|M.avium_104 VSFMLPNWHEAAVIYLAATLAGMVVNPILPSLRDHDLRFVLEDAGAAMVF
MMAR_0303|M.marinum_M VSFMLPNWHEAAVIYLASTLAAMVANPVLPSLREHELRFILEDAGSRMIF
Mb3116|M.bovis_AF2122/97 VALLMPNSVEFCCLFYGAAKLGAVAVPINTRLAAPEVSFILSDSGSKVVI
Rv3089|M.tuberculosis_H37Rv VALLMPNSVEFCCLFYGAAKLGAVAVPINTRLAAPEVSFILSDSGSKVVI
MAB_4377c|M.abscessus_ATCC_199 VGIWAPNCAEWVLVQYATAKIGAILVNINPAYRTHELAYALNQSGVRTLI
MUL_3641|M.ulcerans_Agy99 VLLQLPNSCRFAVALFGLLRAGAIPVMCLPGHRLAELHHFAEVSGAVGLI
MLBr_02546|M.leprae_Br4923 IAILARNHRWFVIANYGCARVGARIILLNSEFSGPQIKEVSEREGAKVII
: : * . . . :: ::
Mflv_1784|M.gilvum_PYR-GCK VPESFGGHDYAAMLDRVAPGLPSPPEVVVVRGE------GRVTLADLLAE
Mvan_4966|M.vanbaalenii_PYR-1 IPQTFGGHDYAAMLDRVVAGLPSPPEVVVVRGEP-----RRPALTDLVSE
TH_3067|M.thermoresistible__bu IPETFGNHDYAAMLDRVTAELPNPPQVVVVRGAVR---PDRVSYDALLSG
MSMEG_5631|M.smegmatis_MC2_155 IPGEFGQHDYAAMLQRVCAHLPTPPQVVVVRGEP-----GPHTAFTALGA
MAV_5212|M.avium_104 VPHRYGGHDYPAMLDRVTAAMSAAPQVVVLRGPDTGRTGRHTPYRCMLGG
MMAR_0303|M.marinum_M APADFRGHDYTSMLRRVAAQLPSRPEVIEVRGQCP---DGVSTFQSLLVG
Mb3116|M.bovis_AF2122/97 YG---APSAPVIDAIRAQADPPGT-------VTDWI---GADSLAERLRS
Rv3089|M.tuberculosis_H37Rv YG---APSAPVIDAIRAQADPPGT-------VTDWI---GADSLAERLRS
MAB_4377c|M.abscessus_ATCC_199 CAKAFKSSDYVAMASQVMPDAPGLRDVVFIGTSDWT---ELVAGAERVTE
MUL_3641|M.ulcerans_Agy99 VAQHAGGFDYRSMAERLVAAHPQLRHVIIDGESG--------PFLPWSSL
MLBr_02546|M.leprae_Br4923 YD-----DEYTKVVSKAEPALGKLRALGVNPDEEEPPGSTDETLAELIAR
: . .
Mflv_1784|M.gilvum_PYR-GCK PADG-VLPAPRPTDTRMILYTSGTTGRPKGVLHTHESLAALIAQLGRFWR
Mvan_4966|M.vanbaalenii_PYR-1 PRDG-TLPDLDPAATRMILYTSGTTGRPKGVLHSHKSLGALIAQLGRYWR
TH_3067|M.thermoresistible__bu PVDRPVLPALDPDAVRMILYTSGTTGRPKGVLHSHNSIHALIHQLREHWL
MSMEG_5631|M.smegmatis_MC2_155 PGGD--LPALDPDSVRMMMHTSGTTGRPKGVLHSHNSIHALICQLRENWM
MAV_5212|M.avium_104 PPGG--LPALDPDAVRMILYTSGTTSRPKGVLHTHNSIHALICQIRDHWA
MMAR_0303|M.marinum_M RETAAPLPTADPDAVRMVLYTSGTTGRPKGVLHTHNSIHALICQIRDHWM
Mb3116|M.bovis_AF2122/97 AAADEPAVECGGDDNLFIMYTSGTTGHPKGVVHTHESVHSAASSWASTID
Rv3089|M.tuberculosis_H37Rv AAADEPAVECGGDDNLFIMYTSGTTGHPKGVVHTHESVHSAASSWASTID
MAB_4377c|M.abscessus_ATCC_199 TALRARMSQLSNTDPINIQYTSGTTGYPKGATLSHRNVLNNGFFVAESIQ
MUL_3641|M.ulcerans_Agy99 SEGATPDIDVDTTSPALLLVSGGTTGTPKLIPRTHDDYVYNATASAQLCR
MLBr_02546|M.leprae_Br4923 SSSAPAPKVRKRAS--IIILTSGTTGTPKGANRNTPASLAPLGGILSKVP
: :.***. ** .
Mflv_1784|M.gilvum_PYR-GCK IDRGDTFLVPSPIAHIGGSIYAFECPLLLG-TEAVLMQRWDPDAAVGLML
Mvan_4966|M.vanbaalenii_PYR-1 IDPGDTFLVPSPIAHIGGSIYAFECPLLLG-TQAVLMQRWDPDAAVALML
TH_3067|M.thermoresistible__bu IDPGDTFLVPSPIAHIGGSIYAFECPLLAG-TTAVLMDRWRPDEAVRLMR
MSMEG_5631|M.smegmatis_MC2_155 IDPGDTFLVPSPIAHIGGSIYAFECPLLLG-TTAVLMQRWNADDGVALMS
MAV_5212|M.avium_104 IDPGDTFLVPSPLAHIGGSIYAFECPLLLG-TTAVLMDRWDPARAVALMT
MMAR_0303|M.marinum_M VEPGDGFLVASPIAHIGGSIYAFECPLLLG-TTAVLLDRWDADRAVQLMT
Mb3116|M.bovis_AF2122/97 VRYRDRLLLPLPMFHVAALTTVIFSAMRG--VTLISMPQFDATKVWSLIV
Rv3089|M.tuberculosis_H37Rv VRYRDRLLLPLPMFHVAALTTVIFSAMRG--VTLISMPQFDATKVWSLIV
MAB_4377c|M.abscessus_ATCC_199 LQAGDRLCIPVPFYHCFGMVMGNLGCTTHGATIVMPAPGFDPGRTLEAIE
MUL_3641|M.ulcerans_Agy99 LTADDVYLVSLPAAHNFPLACPGILGSMAVGATIVFSTDPSPEAAFTLIE
MLBr_02546|M.leprae_Br4923 FRAHEVTLLPAPMFHALGYLHATLAMFLGS--TLVLRRRFKPATVLEDIE
. : :. * * : . :
Mflv_1784|M.gilvum_PYR-GCK EHRVTHMAGATPFLVGLLAAAQRAGTR--LPDLKVFICGGASVPPTLIRS
Mvan_4966|M.vanbaalenii_PYR-1 RHRCTHMAGATPFLDGLLAAAERAGTR--LPDLKVFICGGASVPPSLIRR
TH_3067|M.thermoresistible__bu AEHCTHMAGATPFLDGLLTAARQAGTR--LPDLKVFICGGASVSPSLIRS
MSMEG_5631|M.smegmatis_MC2_155 EHHCTHMAGATPFLDQLLNAAERAGTR--LPDLKVFICGGASVPPSLIRR
MAV_5212|M.avium_104 AHRCTHMAGATPFLQQLLSAAANAGTR--LPDLKVFICGGASVSPSLIRR
MMAR_0303|M.marinum_M SKRCTHMAGATPFLEQLLAAAQRAGTR--LPDLKFFVCGGASVSPSLIRC
Mb3116|M.bovis_AF2122/97 EERVCIGGAVPAILNFMRQVPEFAELD--APDFRYFITGGAPMPEALIK-
Rv3089|M.tuberculosis_H37Rv EERVCIGGAVPAILNFMRQVPEFAELD--APDFRYFITGGAPMPEALIK-
MAB_4377c|M.abscessus_ATCC_199 RERCVGVYGVPTMFIAMLADPGFAHRD--LSTLRTGIMAGSVCPVEVMKR
MUL_3641|M.ulcerans_Agy99 RHGVTVTALVPALAKLWTQACDWEPVI--PKSLRLLQVGGSRLEPEDARQ
MLBr_02546|M.leprae_Br4923 KHQATAMVVVPVMLSRILDTLENLETKSDLSSLRVVFVSGSQLGAELATR
. .. : :: .*:
Mflv_1784|M.gilvum_PYR-GCK AAAYFDKAAVSRVYGSTEVPVTTVGALD--DIDRAADTDGRPGIAEVRIV
Mvan_4966|M.vanbaalenii_PYR-1 AADYFDKAAVSRVYGSTEVPVTTVGSLD--DVDRAADTDGRPGIADVTLV
TH_3067|M.thermoresistible__bu AADYFERAVVTRVYGSTEVPVTTVGVLGPADADRAAETDGRPGIAEVRLA
MSMEG_5631|M.smegmatis_MC2_155 ASTCFEKAVVSRVYGSTEVPVTTVGSIEPGDVEHAACTDGRPGLAEIRLV
MAV_5212|M.avium_104 AAAYFDRAVVTRVYGCTEVPVATVGAPRPQEADYAADTDGRPGIAEIKLV
MMAR_0303|M.marinum_M ATDYFDKAVVTRVYGSTEVPVTTIGAPD--SSAHAAATDGRVGAADVKLV
Mb3116|M.bovis_AF2122/97 -IYAAKNIEVVQGYALTESCGG-GTLLLSEDALRKAGSAGRATMFTDVAV
Rv3089|M.tuberculosis_H37Rv -IYAAKNIEVVQGYALTESCGG-GTLLLSEDALRKAGSAGRATMFTDVAV
MAB_4377c|M.abscessus_ATCC_199 CIDEMHMAGVAIAYGMTETSPVSCQTLFDDDLDRRTATVGRAHPHIEIKI
MUL_3641|M.ulcerans_Agy99 AREALT-PGLQQVFGMAEGLLNFTRLEDPPDLLEHTQGRPLCSADELRIV
MLBr_02546|M.leprae_Br4923 ALEELG-PVIYNMYGSTEIAFATIAGPKDLQINSATVGPVVKGVKVKILD
: :. :* . :
Mflv_1784|M.gilvum_PYR-GCK D-------------GEIRARGPQMFTGYLHP-EDDRESFDDAGYFRTGDL
Mvan_4966|M.vanbaalenii_PYR-1 D-------------GEIRARGPQMLTGYLHP-EDNSESFDAEGFFRTGDL
TH_3067|M.thermoresistible__bu DHAAA-----PPGAGEVLARGPQMLVGYLHP-EDERDAFDADGFFRTGDL
MSMEG_5631|M.smegmatis_MC2_155 D-------------GEIRARGPQMLLGYLHA-EDN--AFDEQGFFRTGDL
MAV_5212|M.avium_104 AHPAA-----PTGDGEICVRGPQMLRGYRHP-EDDAESFDAAGFFRTGDL
MMAR_0303|M.marinum_M A-------------GEIRVRGPQMLVGYRHP-EDEAGSFDAQGYFRTGDL
Mb3116|M.bovis_AF2122/97 RGDDG--VIREHGEGEVVIKSDILLKEYWNRPEATRDAFDN-GWFRTGDI
Rv3089|M.tuberculosis_H37Rv RGDDG--VIREHGEGEVVIKSDILLKEYWNRPEATRDAFDN-GWFRTGDI
MAB_4377c|M.abscessus_ATCC_199 VDPNSGETVQRGQSGELCTRGYSVMLGYWNDEAHTREVLDTDGWMHTGDL
MUL_3641|M.ulcerans_Agy99 DGAGAP--VAPGAEGELLVRGPYTINGYFCADRDNERCFDPAGFYRSGDL
MLBr_02546|M.leprae_Br4923 DSGKE---VPRGQVGRIFVGNAFPFEGYTGG----GGKQIIDGLLSSGDV
*.: . : * * :**:
Mflv_1784|M.gilvum_PYR-GCK GRFTEDN----HLVVTGRAKDIIIRNGENISPKEVEDILVTHPRIAEIAV
Mvan_4966|M.vanbaalenii_PYR-1 GHLTDDG----YLVVTGRAKDLIIRNGENISPKEVEDILVTHPQIAEIAI
TH_3067|M.thermoresistible__bu ARWVDGD----HLVITGRAKDIIIRNGENISPKEVEDLLIGHPGVAEIAV
MSMEG_5631|M.smegmatis_MC2_155 GRWVDGD----YLTVTGRAKDLIIRNGENISPKEIEDILVGELGINEIAV
MAV_5212|M.avium_104 GRWALADSADRYLVVTGRAKDVIIRSGENISAKEVEDLLADHPGIAEIAV
MMAR_0303|M.marinum_M GHWVDDE----YLVVTGRAKDIIIRNGENISPKEIEDILITHPGIADVAI
Mb3116|M.bovis_AF2122/97 GEIDDEG----YLYIKDRLKDMIISGGENVYPAEIESVIIGVPGVSEVAV
Rv3089|M.tuberculosis_H37Rv GEIDDEG----YLYIKDRLKDMIISGGENVYPAEIESVIIGVPGVSEVAV
MAB_4377c|M.abscessus_ATCC_199 AVMRDDG----YCTIIGRLKDMVIRGGENIYPREIEEFLLTHPDIEDVHV
MUL_3641|M.ulcerans_Agy99 VRLRDDG----YLQVTGRVKDVICRCGETISAQDLEEQLLSHPAIRSAAA
MLBr_02546|M.leprae_Br4923 GYFDEHD----LLYISGRDDEMIVSGGENVFPAEVEDLISGHPEVVEATA
: .* .::: **.: . ::*. : : .
Mflv_1784|M.gilvum_PYR-GCK VGIPDPRTGERACAALVTTEDP--PPDIAELKDFLTDRGVARFKVPEQIH
Mvan_4966|M.vanbaalenii_PYR-1 VGIPDLRTGERACAVIVAADSA--APDVAEIRDFLVARGVAKFKAPERVV
TH_3067|M.thermoresistible__bu VGVPDPRTGERACAAIVPTGRP--GPDVAELREFLLARGVAKFKAPEQVV
MSMEG_5631|M.smegmatis_MC2_155 VGLPDERTGERACAVVVTEDGA--RPDVSALAEVLTKAGVAKFKIPEQVE
MAV_5212|M.avium_104 VGLPDERTGERACAVIVPTPGA--SPDVASLLALLVSKGVAKFKAPEQVV
MMAR_0303|M.marinum_M VGLPDDRTGERACAVIVAAADP--PPGLPGVRAFLEGAGVARFKIPEQVV
Mb3116|M.bovis_AF2122/97 IGLPDEKWGEIAAAIVVADQN----EVSEQQIVEYCGTRLARYKLPKKVI
Rv3089|M.tuberculosis_H37Rv IGLPDEKWGEIAAAIVVADQN----EVSEQQIVEYCGTRLARYKLPKKVI
MAB_4377c|M.abscessus_ATCC_199 VGVPDEKYGEELCAWVRMRPDR--VVIDAVAIRAFASGRLAHYKIPRYVH
MUL_3641|M.ulcerans_Agy99 VPLPDPDMGELICAAIVFAGPPQTPQTLSGLNDYLEQRGVAAHIRIDRLV
MLBr_02546|M.leprae_Br4923 IGVDDKEWGARLRAFVVKKQDA---TIDEDAIKAYVRDHLARYKVPREVI
: : * * * : :* . :
Mflv_1784|M.gilvum_PYR-GCK VVGALPKNDAGKVLKHVLKAELTKES-----------
Mvan_4966|M.vanbaalenii_PYR-1 VVDALPKNDAGKVLKHAIRAVLLEKPREGEP------
TH_3067|M.thermoresistible__bu FFDALPKNDAGKVLKHQIQATLTAAEVSG--------
MSMEG_5631|M.smegmatis_MC2_155 IWDALPKNDAGKVLKHQIRTQLSKVE-----------
MAV_5212|M.avium_104 LWDALPKNDAGKVLKHRIRAALSKDG-----------
MMAR_0303|M.marinum_M IWDHLPKNHAGKIVKHRIKAALMKAEQ----------
Mb3116|M.bovis_AF2122/97 FAEAIPRNPTGKILKTVLREQYSATVPK---------
Rv3089|M.tuberculosis_H37Rv FAEAIPRNPTGKILKTVLREQYSATVPK---------
MAB_4377c|M.abscessus_ATCC_199 VVESFPMTVTGKVRKIEMRQQTIQIFGLPEPGRTDEQ
MUL_3641|M.ulcerans_Agy99 AMPSLPTTPVGKIDKKAIASQILTTTAT---------
MLBr_02546|M.leprae_Br4923 FLEELPRNPTGKVLKRELRDFEVH-------------
:* . .**: * :