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MRAEDAYERGLWVRTTLADTLAEAARDTPQRVLLIDGDVRLTCAELHSRAKALAHEMAARMPAGSVVSFM LPNWHEAAIVYLASTLAGMVINPILPSLRDHELRFILDDAASRMIFIPGEFGQHDYAAMLQRVCAHLPTP PQVVVVRGEPGPHTAFTALGAPGGDLPALDPDSVRMMMHTSGTTGRPKGVLHSHNSIHALICQLRENWMI DPGDTFLVPSPIAHIGGSIYAFECPLLLGTTAVLMQRWNADDGVALMSEHHCTHMAGATPFLDQLLNAAE RAGTRLPDLKVFICGGASVPPSLIRRASTCFEKAVVSRVYGSTEVPVTTVGSIEPGDVEHAACTDGRPGL AEIRLVDGEIRARGPQMLLGYLHAEDNAFDEQGFFRTGDLGRWVDGDYLTVTGRAKDLIIRNGENISPKE IEDILVGELGINEIAVVGLPDERTGERACAVVVTEDGARPDVSALAEVLTKAGVAKFKIPEQVEIWDALP KNDAGKVLKHQIRTQLSKVE
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. smegmatis MC2 155 | MSMEG_5631 | - | - | 100% (510) | AMP-dependent synthetase and ligase |
| M. smegmatis MC2 155 | MSMEG_4774 | - | 2e-63 | 36.01% (511) | AMP-dependent synthetase and ligase |
| M. smegmatis MC2 155 | MSMEG_4112 | - | 3e-52 | 30.64% (532) | cyclohexanecarboxylate-CoA ligase |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb3591 | fadD3 | 1e-48 | 31.67% (502) | acyl-CoA synthetase |
| M. gilvum PYR-GCK | Mflv_1784 | - | 0.0 | 68.54% (499) | AMP-dependent synthetase and ligase |
| M. tuberculosis H37Rv | Rv3561 | fadD3 | 4e-49 | 31.87% (502) | acyl-CoA synthetase |
| M. leprae Br4923 | MLBr_01051 | xclC | 3e-32 | 31.94% (360) | acyl-CoA synthetase |
| M. abscessus ATCC 19977 | MAB_4377c | - | 9e-49 | 29.76% (541) | AMP-binding domain protein |
| M. marinum M | MMAR_0303 | - | 0.0 | 66.99% (515) | acyl-CoA synthetase |
| M. avium 104 | MAV_5212 | - | 0.0 | 68.20% (522) | AMP-binding enzyme, putative |
| M. thermoresistible (build 8) | TH_3067 | - | 0.0 | 71.32% (523) | PUTATIVE AMP-binding enzyme, putative |
| M. ulcerans Agy99 | MUL_4126 | fadD3 | 5e-43 | 29.82% (503) | acyl-CoA synthetase |
| M. vanbaalenii PYR-1 | Mvan_4966 | - | 0.0 | 71.52% (495) | AMP-dependent synthetase and ligase |
CLUSTAL 2.0.9 multiple sequence alignment
Mb3591|M.bovis_AF2122/97 ------------------------------MINDLRTVPAALDRLVRQLP
Rv3561|M.tuberculosis_H37Rv ------------------------------MINDLRTVPAALDRLVRQLP
MUL_4126|M.ulcerans_Agy99 ------------------------------MTSDPRTVPTALDRLVRRLP
MAB_4377c|M.abscessus_ATCC_199 --------------------MKAYDAGATLSPIIEETIGENFERIARAHP
Mflv_1784|M.gilvum_PYR-GCK ---------------------------------MTGTLVEALRDAARDTP
Mvan_4966|M.vanbaalenii_PYR-1 ---------------------------------MT-TLADALRDAARDTP
TH_3067|M.thermoresistible__bu ----MTETPGAGYRTDRPGHAERAAQAYARGLWVPTTLAEALAEAARTTP
MSMEG_5631|M.smegmatis_MC2_155 ---------------------MRAEDAYERGLWVRTTLADTLAEAARDTP
MAV_5212|M.avium_104 ------------MTARAAPDTAQTSLAYRRGWWVRSTLADAARAAAQRTP
MMAR_0303|M.marinum_M MGGPKVPQRRLIDGRWVRWDADRAAGAYAHRWWVRRTLADALREAAQSTP
MLBr_01051|M.leprae_Br4923 ------------------------------------------MLLASLNP
. *
Mb3591|M.bovis_AF2122/97 DHTALIAE--DRRFTSTELRDAVYGAAAALIALGVEPADRVAIWSPNTWH
Rv3561|M.tuberculosis_H37Rv DHTALIAE--DRRFTSTELRDAVYGAAAALIALGVEPADRVAIWSPNTWH
MUL_4126|M.ulcerans_Agy99 NHDALVTD--ERSFTVSELNDEVYRAAAALIELGVKPAARVGIWSPNTWH
MAB_4377c|M.abscessus_ATCC_199 DVGALVDVSGDRRWTYRELDAEINRVARGLMSLGVAAGDRVGIWAPNCAE
Mflv_1784|M.gilvum_PYR-GCK DRVLVRDD--AVALTCAELLSAATGLAHALTRR-MPAGSVVSFTVPNWHE
Mvan_4966|M.vanbaalenii_PYR-1 DRILLRDD--EASLSSAELLSAATNLAHALVRR-MPVGSVVSFAVPNWHE
TH_3067|M.thermoresistible__bu DRTVLVDG--AVRLDCRSLHARATALAGALLRR-IPAGAVVSFMLPNWHE
MSMEG_5631|M.smegmatis_MC2_155 QRVLLIDG--DVRLTCAELHSRAKALAHEMAAR-MPAGSVVSFMLPNWHE
MAV_5212|M.avium_104 ERIALVDN--EVRLNCATLYAQAGELAAALLAR-IPAGSVVSFMLPNWHE
MMAR_0303|M.marinum_M QRVALVDA--DRQLDCQELHRQAMALAQHLRSR-MPAGSVVSFMLPNWHE
MLBr_01051|M.leprae_Br4923 SVVTATDIADAVCVDGVVLSRSGLVGAATSVAERVGGARLVAVLATPTAS
. * * : . *.. .
Mb3591|M.bovis_AF2122/97 WVVACLAIHHAGAAVVPLNTRYTATEATDILDRAGAPVLFAAGLFLGADR
Rv3561|M.tuberculosis_H37Rv WVVACLAIHHAGAAVVPLNTRYTATEATDILDRAGAPVLFAAGLFLGADR
MUL_4126|M.ulcerans_Agy99 WVVACLAIHHVGAAMVPLNTRYTADEAADILARTEAPVLFAMGHFLGSDR
MAB_4377c|M.abscessus_ATCC_199 WVLVQYATAKIGAILVNINPAYRTHELAYALNQSGVRTLICAKAFKSSDY
Mflv_1784|M.gilvum_PYR-GCK AAIIYLGATLAGMVVNPILPSLRERELSFILDDADSRMIFVPESFGGHDY
Mvan_4966|M.vanbaalenii_PYR-1 AAVIYLGATLAGMVVNPILPSLRERELSFILADAGSRAIFIPQTFGGHDY
TH_3067|M.thermoresistible__bu AAVIYLGATLAGMVVNPILPSLRDRELRFILDDADSRMIFIPETFGNHDY
MSMEG_5631|M.smegmatis_MC2_155 AAIVYLASTLAGMVINPILPSLRDHELRFILDDAASRMIFIPGEFGQHDY
MAV_5212|M.avium_104 AAVIYLAATLAGMVVNPILPSLRDHDLRFVLEDAGAAMVFVPHRYGGHDY
MMAR_0303|M.marinum_M AAVIYLASTLAAMVANPVLPSLREHELRFILEDAGSRMIFAPADFRGHDY
MLBr_01051|M.leprae_Br4923 TVLAITGCLIAGVPVVPVPADIGVVERRHMLTDSGAQAWLGPGPSADSAP
.: . . : . : * : :
Mb3591|M.bovis_AF2122/97 AAGLDRAALPALRHVVRVPVEADDG--TWDEFIATGAGALDAVAARAAAV
Rv3561|M.tuberculosis_H37Rv AAGLDRAALPALRHVVRVPVEADDG--TWDEFIATGAGALDAVAARAAAV
MUL_4126|M.ulcerans_Agy99 VAGLDRKALPALRHIVRIPIEEPDG--TWDEFIETGT-DTGAVTARAAAV
MAB_4377c|M.abscessus_ATCC_199 VA-MASQVMPDAPGLRDVVFIGTSD--WTELVAGAERVTETALRARMSQL
Mflv_1784|M.gilvum_PYR-GCK AAMLDRVAPGLPSPPEVVVVRGE------GRVTLADLLAEPADG-VLPAP
Mvan_4966|M.vanbaalenii_PYR-1 AAMLDRVVAGLPSPPEVVVVRGEP-----RRPALTDLVSEPRDG-TLPDL
TH_3067|M.thermoresistible__bu AAMLDRVTAELPNPPQVVVVRGAVR---PDRVSYDALLSGPVDRPVLPAL
MSMEG_5631|M.smegmatis_MC2_155 AAMLQRVCAHLPTPPQVVVVRGEP-----GPHTAFTALGAPGGD--LPAL
MAV_5212|M.avium_104 PAMLDRVTAAMSAAPQVVVLRGPDTGRTGRHTPYRCMLGGPPGG--LPAL
MMAR_0303|M.marinum_M TSMLRRVAAQLPSRPEVIEVRGQCP---DGVSTFQSLLVGRETAAPLPTA
MLBr_01051|M.leprae_Br4923 DGLPHIPVQLDARSWNR-----------------------------YPEP
. .
Mb3591|M.bovis_AF2122/97 APQDVSDILFTSGTTGRSKGVLCAHRQSLSASASWAANGKITSDDRYLCI
Rv3561|M.tuberculosis_H37Rv APQDVSDILFTSGTTGRSKGVLCAHRQSLSASASWAANGKITSDDRYLCI
MUL_4126|M.ulcerans_Agy99 SPDDVSDILFTSGTTGRSKGVLCAHRQSLSASASWAANGKITADDRHLCI
MAB_4377c|M.abscessus_ATCC_199 SNTDPINIQYTSGTTGYPKGATLSHRNVLNNGFFVAESIQLQAGDRLCIP
Mflv_1784|M.gilvum_PYR-GCK RPTDTRMILYTSGTTGRPKGVLHTHESLAALIAQLGRFWRIDRGDTFLVP
Mvan_4966|M.vanbaalenii_PYR-1 DPAATRMILYTSGTTGRPKGVLHSHKSLGALIAQLGRYWRIDPGDTFLVP
TH_3067|M.thermoresistible__bu DPDAVRMILYTSGTTGRPKGVLHSHNSIHALIHQLREHWLIDPGDTFLVP
MSMEG_5631|M.smegmatis_MC2_155 DPDSVRMMMHTSGTTGRPKGVLHSHNSIHALICQLRENWMIDPGDTFLVP
MAV_5212|M.avium_104 DPDAVRMILYTSGTTSRPKGVLHTHNSIHALICQIRDHWAIDPGDTFLVP
MMAR_0303|M.marinum_M DPDAVRMVLYTSGTTGRPKGVLHTHNSIHALICQIRDHWMVEPGDGFLVA
MLBr_01051|M.leprae_Br4923 SPDATAMVIYTSGTTGPPKGVLLSRRAIAVDLDALAQAWQWTADDVLVHG
: .*****. .**. ::. .*
Mb3591|M.bovis_AF2122/97 NPFFHNFGYKAGILACLQTGATLIPHVT-FDPLHALRAIERHRITVLPGP
Rv3561|M.tuberculosis_H37Rv NPFFHNFGYKAGILACLQTGATLIPHVT-FDPLHALRAIERHRITVLPGP
MUL_4126|M.ulcerans_Agy99 NPFFHNFGYKAGILACLQTGATLFPHLT-FDPLRTLQAIERHRITVLPGP
MAB_4377c|M.abscessus_ATCC_199 VPFYHCFGMVMGNLGCTTHGATIVMPAPGFDPGRTLEAIERERCVGVYGV
Mflv_1784|M.gilvum_PYR-GCK SPIAHIGGSIYAFECPLLLGTEAVLMQR-WDPDAAVGLMLEHRVTHMAGA
Mvan_4966|M.vanbaalenii_PYR-1 SPIAHIGGSIYAFECPLLLGTQAVLMQR-WDPDAAVALMLRHRCTHMAGA
TH_3067|M.thermoresistible__bu SPIAHIGGSIYAFECPLLAGTTAVLMDR-WRPDEAVRLMRAEHCTHMAGA
MSMEG_5631|M.smegmatis_MC2_155 SPIAHIGGSIYAFECPLLLGTTAVLMQR-WNADDGVALMSEHHCTHMAGA
MAV_5212|M.avium_104 SPLAHIGGSIYAFECPLLLGTTAVLMDR-WDPARAVALMTAHRCTHMAGA
MMAR_0303|M.marinum_M SPIAHIGGSIYAFECPLLLGTTAVLLDR-WDADRAVQLMTSKRCTHMAGA
MLBr_01051|M.leprae_Br4923 LPLFHVHGLVLGLLGSLRIGNRFVHTGK-PTPTAYAQACSEAGGSLYFGV
*: * * . * . . *
Mb3591|M.bovis_AF2122/97 PTIYQSLLDHPARKDFDLSSLRFAVTGAATVPVVLVERMQSELDIDIVLT
Rv3561|M.tuberculosis_H37Rv PTIYQSLLDHPARKDFDLSSLRFAVTGAATVPVVLVERMQSELDIDIVLT
MUL_4126|M.ulcerans_Agy99 PTIYQTLLDHPARDDYDLSSLRFAVTGAATVPVVLVERMQSELDIDIVLT
MAB_4377c|M.abscessus_ATCC_199 PTMFIAMLADPGFAHRDLSTLRTGIMAGSVCPVEVMKRCIDEMHMAGVAI
Mflv_1784|M.gilvum_PYR-GCK TPFLVGLLAAAQRAGTRLPDLKVFICGGASVPPTLIRSAAAYFDKAAVSR
Mvan_4966|M.vanbaalenii_PYR-1 TPFLDGLLAAAERAGTRLPDLKVFICGGASVPPSLIRRAADYFDKAAVSR
TH_3067|M.thermoresistible__bu TPFLDGLLTAARQAGTRLPDLKVFICGGASVSPSLIRSAADYFERAVVTR
MSMEG_5631|M.smegmatis_MC2_155 TPFLDQLLNAAERAGTRLPDLKVFICGGASVPPSLIRRASTCFEKAVVSR
MAV_5212|M.avium_104 TPFLQQLLSAAANAGTRLPDLKVFICGGASVSPSLIRRAAAYFDRAVVTR
MMAR_0303|M.marinum_M TPFLEQLLAAAQRAGTRLPDLKFFVCGGASVSPSLIRCATDYFDKAVVTR
MLBr_01051|M.leprae_Br4923 PTVWSRLVADEAVAR-ALRPARLLVSGSASLPVPVFDRLAHLTGHRPIER
... :: * : : ..: . :. :
Mb3591|M.bovis_AF2122/97 AYGLTEANGMGTMCRPEDDAVTVATTCGRPFADFELRIADD---------
Rv3561|M.tuberculosis_H37Rv AYGLTEANGMGTMCRPEDDAVTVATTCGRPFADFELRIADD---------
MUL_4126|M.ulcerans_Agy99 AYGLTEANGMGTMCRADDDALTVATTCGRPFADFELRIGPDT--------
MAB_4377c|M.abscessus_ATCC_199 AYGMTETSPVSCQTLFDDDLDRRTATVGRAHPHIEIKIVDPNSGETVQR-
Mflv_1784|M.gilvum_PYR-GCK VYGSTEVPVTTVGALD--DIDRAADTDGRP-GIAEVRIVD----------
Mvan_4966|M.vanbaalenii_PYR-1 VYGSTEVPVTTVGSLD--DVDRAADTDGRP-GIADVTLVD----------
TH_3067|M.thermoresistible__bu VYGSTEVPVTTVGVLGPADADRAAETDGRP-GIAEVRLADHAAAPP----
MSMEG_5631|M.smegmatis_MC2_155 VYGSTEVPVTTVGSIEPGDVEHAACTDGRP-GLAEIRLVD----------
MAV_5212|M.avium_104 VYGCTEVPVATVGAPRPQEADYAADTDGRP-GIAEIKLVAHPAAPT----
MMAR_0303|M.marinum_M VYGSTEVPVTTIGAPD--SSAHAAATDGRV-GAADVKLVA----------
MLBr_01051|M.leprae_Br4923 -YGSTES---LITLSTLADGERRAGWVGLPLAGVQTRLVDESGGPVPYDG
** ** . : * : :
Mb3591|M.bovis_AF2122/97 ---GEVLLRGPNVMVGYLDDTEATAAAIDADGWLHTGDIGAVDQAG----
Rv3561|M.tuberculosis_H37Rv ---GEVLLRGPNVMVGYLDDTEATAAAIDADGWLHTGDIGAVDQAG----
MUL_4126|M.ulcerans_Agy99 ---GEVLLRGPNVMLGYLDDPRATAAAIDADGWLHTGDIGVLDEAG----
MAB_4377c|M.abscessus_ATCC_199 GQSGELCTRGYSVMLGYWNDEAHTREVLDTDGWMHTGDLAVMRDDG----
Mflv_1784|M.gilvum_PYR-GCK ---GEIRARGPQMFTGYLHP-EDDRESFDDAGYFRTGDLGRFTEDN----
Mvan_4966|M.vanbaalenii_PYR-1 ---GEIRARGPQMLTGYLHP-EDNSESFDAEGFFRTGDLGHLTDDG----
TH_3067|M.thermoresistible__bu -GAGEVLARGPQMLVGYLHP-EDERDAFDADGFFRTGDLARWVDGD----
MSMEG_5631|M.smegmatis_MC2_155 ---GEIRARGPQMLLGYLHA-EDN--AFDEQGFFRTGDLGRWVDGD----
MAV_5212|M.avium_104 -GDGEICVRGPQMLRGYRHP-EDDAESFDAAGFFRTGDLGRWALADSADR
MMAR_0303|M.marinum_M ---GEIRVRGPQMLVGYRHP-EDEAGSFDAQGYFRTGDLGHWVDDE----
MLBr_01051|M.leprae_Br4923 ETVGRLQVRSPTMFGGYLNRPEATAEAFDEDGWYRTGDVAVVDSGG---M
*.: *. :: ** . :* *: :***:.
Mb3591|M.bovis_AF2122/97 NLRINDRLKDMYICGGFNVYPAEVEQVLARMDGVADAAVIGVPDQRLGEV
Rv3561|M.tuberculosis_H37Rv NLRITDRLKDMYICGGFNVYPAEVEQVLARMDGVADAAVIGVPDQRLGEV
MUL_4126|M.ulcerans_Agy99 NLRITDRLKDMYICGGFNVYPAEVEQVLARLDGVADVAVIGIPDHRLGEV
MAB_4377c|M.abscessus_ATCC_199 YCTIIGRLKDMVIRGGENIYPREIEEFLLTHPDIEDVHVVGVPDEKYGEE
Mflv_1784|M.gilvum_PYR-GCK HLVVTGRAKDIIIRNGENISPKEVEDILVTHPRIAEIAVVGIPDPRTGER
Mvan_4966|M.vanbaalenii_PYR-1 YLVVTGRAKDLIIRNGENISPKEVEDILVTHPQIAEIAIVGIPDLRTGER
TH_3067|M.thermoresistible__bu HLVITGRAKDIIIRNGENISPKEVEDLLIGHPGVAEIAVVGVPDPRTGER
MSMEG_5631|M.smegmatis_MC2_155 YLTVTGRAKDLIIRNGENISPKEIEDILVGELGINEIAVVGLPDERTGER
MAV_5212|M.avium_104 YLVVTGRAKDVIIRSGENISAKEVEDLLADHPGIAEIAVVGLPDERTGER
MMAR_0303|M.marinum_M YLVVTGRAKDIIIRNGENISPKEIEDILITHPGIADVAIVGLPDDRTGER
MLBr_01051|M.leprae_Br4923 HRIVGRESVDLIKLGGYRIGAGEIEMALLGHPDVREVAVVGLPDEDLGQR
: . *: .* .: . *:* * : : ::*:** *:
Mb3591|M.bovis_AF2122/97 GRAFVVARPG-TGLDEASVIAYTREHLANFKTPRSVRFVDVLPRNAAGKV
Rv3561|M.tuberculosis_H37Rv GRAFVVARPG-TGLDEASVIAYTREHLANFKTPRSVRFVDVLPRNAAGKV
MUL_4126|M.ulcerans_Agy99 GRAFIVARPG-FNLDEKSVIDYTREHLANFKAPRSVRFVDTLPRNAGGKV
MAB_4377c|M.abscessus_ATCC_199 LCAWVRMRPDRVVIDAVAIRAFASGRLAHYKIPRYVHVVESFPMTVTGKV
Mflv_1784|M.gilvum_PYR-GCK ACAALVTTEDPPPDIAELKDFLTDRGVARFKVPEQIHVVGALPKNDAGKV
Mvan_4966|M.vanbaalenii_PYR-1 ACAVIVAADSAAPDVAEIRDFLVARGVAKFKAPERVVVVDALPKNDAGKV
TH_3067|M.thermoresistible__bu ACAAIVPTGRPGPDVAELREFLLARGVAKFKAPEQVVFFDALPKNDAGKV
MSMEG_5631|M.smegmatis_MC2_155 ACAVVVTEDGARPDVSALAEVLTKAGVAKFKIPEQVEIWDALPKNDAGKV
MAV_5212|M.avium_104 ACAVIVPTPGASPDVASLLALLVSKGVAKFKAPEQVVLWDALPKNDAGKV
MMAR_0303|M.marinum_M ACAVIVAAADPPPGLPGVRAFLEGAGVARFKIPEQVVIWDHLPKNHAGKI
MLBr_01051|M.leprae_Br4923 IVAFVVGAEA---LDADELINYVAQQLSIHKRPREVRFVDALPRNAMGKV
* : :: .* *. : . :* . **:
Mb3591|M.bovis_AF2122/97 SKPQLRELG---------------
Rv3561|M.tuberculosis_H37Rv SKPQLRELG---------------
MUL_4126|M.ulcerans_Agy99 VKPQLRELA---------------
MAB_4377c|M.abscessus_ATCC_199 RKIEMRQQTIQIFGLPEPGRTDEQ
Mflv_1784|M.gilvum_PYR-GCK LKHVLKAELTKES-----------
Mvan_4966|M.vanbaalenii_PYR-1 LKHAIRAVLLEKPREGEP------
TH_3067|M.thermoresistible__bu LKHQIQATLTAAEVSG--------
MSMEG_5631|M.smegmatis_MC2_155 LKHQIRTQLSKVE-----------
MAV_5212|M.avium_104 LKHRIRAALSKDG-----------
MMAR_0303|M.marinum_M VKHRIKAALMKAEQ----------
MLBr_01051|M.leprae_Br4923 LKKQLLFEG---------------
* :