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MRRIPEELVRRYEQEGYWTPETLGELLARGLDSAPGAGFHVHSAVRPYAGTFAEVERDARRLAAGLRERG VAAGDVVALQLPNWREAAVTFWASAFLGAVVVPVVHFYGRKELAHILTTAKPRVFVTTAQFGRLQFHPDV CAGVPIVALVGDGPDSGADGGWEPFEDLLAAEPMAGTVAADPAGPALIAFTSGTTRDPKGVIHSHQTLSF ETRQLLENYPPDRGRQLTATPVGHFIGMLGAFLIPVLEGSPIDLCDVWDPGRVLQLMETEGLSIGGGPPY FVTSLLDHPQCRPEHVRRFKTVGLGGSTVPAAVTRRLSDLGMFVFRSYGSTEHPSITGSRPSAPEDKRLF TDGDARPGVEIKLGPDGEIFSRGPDLCLGYVDDDLTAQAFDEDGWYHTGDIGVLDEDGYLTITDRKADVI IRGGENISALEVEEILLGMPAVAEAVVVAAPDERLGEHTAAVLRIRDGHVMPSLDDVRAHFRAAGAATQK WPEELHRVPEGQDFPRTASGKVQKYVIRQQVAAGMHGVVAASQK
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. gilvum PYR-GCK | Mflv_2523 | - | - | 100% (534) | AMP-dependent synthetase and ligase |
| M. gilvum PYR-GCK | Mflv_1784 | - | 1e-60 | 34.44% (482) | AMP-dependent synthetase and ligase |
| M. gilvum PYR-GCK | Mflv_4867 | - | 9e-51 | 31.00% (542) | acyl-CoA synthetase |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb3591 | fadD3 | 2e-47 | 33.55% (471) | acyl-CoA synthetase |
| M. tuberculosis H37Rv | Rv3561 | fadD3 | 4e-48 | 33.76% (471) | acyl-CoA synthetase |
| M. leprae Br4923 | MLBr_02546 | fadD2 | 8e-28 | 25.81% (496) | acyl-CoA synthetase |
| M. abscessus ATCC 19977 | MAB_4377c | - | 1e-53 | 32.58% (528) | AMP-binding domain protein |
| M. marinum M | MMAR_0303 | - | 4e-59 | 33.66% (514) | acyl-CoA synthetase |
| M. avium 104 | MAV_2638 | - | 2e-82 | 39.71% (486) | AMP-dependent synthetase and ligase |
| M. smegmatis MC2 155 | MSMEG_4774 | - | 0.0 | 75.05% (521) | AMP-dependent synthetase and ligase |
| M. thermoresistible (build 8) | TH_2816 | - | 0.0 | 75.84% (534) | PUTATIVE AMP-dependent synthetase and ligase |
| M. ulcerans Agy99 | MUL_4126 | fadD3 | 5e-50 | 33.61% (488) | acyl-CoA synthetase |
| M. vanbaalenii PYR-1 | Mvan_4135 | - | 0.0 | 81.46% (534) | AMP-dependent synthetase and ligase |
CLUSTAL 2.0.9 multiple sequence alignment
Mflv_2523|M.gilvum_PYR-GCK -------------------------MRRIPEELVRRYEQEGYWTPETLGE
Mvan_4135|M.vanbaalenii_PYR-1 -------------------------MRRIPAQLVERYQREGYWTQETLGD
MSMEG_4774|M.smegmatis_MC2_155 -------------------------MRDIPAELTKRYVEEGWWTPETLGQ
TH_2816|M.thermoresistible__bu -------------------------MRSIPAELRRRYEENGWWTQESLGD
MAV_2638|M.avium_104 -------------------------MPFDHRQLRDKWYSAGWYSDRTCLD
MMAR_0303|M.marinum_M ----------MGGPKVPQRRLIDGRWVRWDADRAAGAYAHRWWVRRTLAD
Mb3591|M.bovis_AF2122/97 ----------------------------------------MINDLRTVPA
Rv3561|M.tuberculosis_H37Rv ----------------------------------------MINDLRTVPA
MUL_4126|M.ulcerans_Agy99 ----------------------------------------MTSDPRTVPT
MAB_4377c|M.abscessus_ATCC_199 ------------------------------MKAYDAGATLSPIIEETIGE
MLBr_02546|M.leprae_Br4923 MTKLRHYLERGAAEVHYLRSIFESGALGFEPPLTYAAMAVAMRKWGDLAM
Mflv_2523|M.gilvum_PYR-GCK LLARGLDSAPGAGFHVHSAVRPYAGTFAEVERDARRLAAGLRERGVAAGD
Mvan_4135|M.vanbaalenii_PYR-1 MLARGLAGSPEAGFCVHSSIRPYRGTFGEVERDARRLAAGLRERGVGPGD
MSMEG_4774|M.smegmatis_MC2_155 MLAEGLAAGPDVVFCVHSEVRPYTGTFGEVETLARRLAAGLRARGVGPGD
TH_2816|M.thermoresistible__bu LLAEGLRAAPDAGFRVHSDVRPYTGTFADVEERARRLAAGLRARGVGPGD
MAV_2638|M.avium_104 AFEAGAVEHKDVPLTFVIGDSVSSPTVCEIHDRAVALAASLQRLGVRDGD
MMAR_0303|M.marinum_M ALREAAQSTPQRVALVDADR---QLDCQELHRQAMALAQHLRSR-MPAGS
Mb3591|M.bovis_AF2122/97 ALDRLVRQLPDHTALIAE---DRRFTSTELRDAVYGAAAALIALGVEPAD
Rv3561|M.tuberculosis_H37Rv ALDRLVRQLPDHTALIAE---DRRFTSTELRDAVYGAAAALIALGVEPAD
MUL_4126|M.ulcerans_Agy99 ALDRLVRRLPNHDALVTD---ERSFTVSELNDEVYRAAAALIELGVKPAA
MAB_4377c|M.abscessus_ATCC_199 NFERIARAHPDVGALVDVSG-DRRWTYRELDAEINRVARGLMSLGVAAGD
MLBr_02546|M.leprae_Br4923 LPAMPARRSPHRAALIDEEG---TLTYKELDRAAHALANGLIAKGVRGGD
. :: * * : .
Mflv_2523|M.gilvum_PYR-GCK VVALQLPNWREAAVTFWASAFLGAVVVPVVHFYGRKELAHILTTAKPRVF
Mvan_4135|M.vanbaalenii_PYR-1 VVAMQLPNWREAAVTFWASAFLGAVVVPIVHFYGRRELAHIMATAKPRVF
MSMEG_4774|M.smegmatis_MC2_155 VIAFQLPNWMEAAAAFWATSFLGAVTVPIVHFYGRKELAHILSTAKPKVF
TH_2816|M.thermoresistible__bu VIAFQLPNWVEAAMTFWASAFLGAVVVPVVHFYGRKELGHILRTARPKVF
MAV_2638|M.avium_104 AVAVQLTNRVECAVAYQAVLLCGAVLVPIVHIYGMAEVAFVLDQSAAKVL
MMAR_0303|M.marinum_M VVSFMLPNWHEAAVIYLASTLAAMVANPVLPSLREHELRFILEDAGSRMI
Mb3591|M.bovis_AF2122/97 RVAIWSPNTWHWVVACLAIHHAGAAVVPLNTRYTATEATDILDRAGAPVL
Rv3561|M.tuberculosis_H37Rv RVAIWSPNTWHWVVACLAIHHAGAAVVPLNTRYTATEATDILDRAGAPVL
MUL_4126|M.ulcerans_Agy99 RVGIWSPNTWHWVVACLAIHHVGAAMVPLNTRYTADEAADILARTEAPVL
MAB_4377c|M.abscessus_ATCC_199 RVGIWAPNCAEWVLVQYATAKIGAILVNINPAYRTHELAYALNQSGVRTL
MLBr_02546|M.leprae_Br4923 GIAILARNHRWFVIANYGCARVGARIILLNSEFSGPQIKEVSEREGAKVI
:.. * . . . : : :
Mflv_2523|M.gilvum_PYR-GCK VTTAQFGRLQFHPDVC-AGVPIV-ALVGDGPDSGADG-GWEPFEDLLAAE
Mvan_4135|M.vanbaalenii_PYR-1 VTAEEFGRMRYQPDLC-GDVAVV-GLVG--AAHPAAG-NEHAFEQLLADE
MSMEG_4774|M.smegmatis_MC2_155 LTTERFGRMSFQPDLC-AQVPIV-GLVG-----------ESSFTDLLAER
TH_2816|M.thermoresistible__bu ITAAEFGRMRYQPDLC-ADVPIV-GVVG-----------QHGFDDLVADE
MAV_2638|M.avium_104 IMPERHGSIVYSKRLQ-DLSRID-SLQHVVMLDAEPGEGYLAWSQLDTPA
MMAR_0303|M.marinum_M FAPADFRGHDYTSMLRRVAAQLP-SRPEVIEVRGQCPDGVSTFQSLLVGR
Mb3591|M.bovis_AF2122/97 FAAGLFLGADRAAGLDRAALPALRHVVRVPVEADDGTWDEFIATGAGALD
Rv3561|M.tuberculosis_H37Rv FAAGLFLGADRAAGLDRAALPALRHVVRVPVEADDGTWDEFIATGAGALD
MUL_4126|M.ulcerans_Agy99 FAMGHFLGSDRVAGLDRKALPALRHIVRIPIEEPDGTWDEFIETGT-DTG
MAB_4377c|M.abscessus_ATCC_199 ICAKAFKSSDYVA-MASQVMPDAPGLRDVVFIGTSDWTELVAGAERVTET
MLBr_02546|M.leprae_Br4923 IYDDEYTKVVSKAEPALGKLRALGVNPD---EEEPPGSTDETLAELIARS
. .
Mflv_2523|M.gilvum_PYR-GCK PMAG-TVAADPAGPALIAFTSGTTRDPKGVIHSHQTLSFETR---QLLEN
Mvan_4135|M.vanbaalenii_PYR-1 PMAG-TVAADPAAPTLIAFTSGTTRDPKGVIHSHQTLSCETR---QLLEN
MSMEG_4774|M.smegmatis_MC2_155 PMSG-TLDTDPAAPALIAFTSGTTRDPKGVIHSHQTLSCETR---QLLAN
TH_2816|M.thermoresistible__bu PMTG-TLATDPAGPALIAFTSGTTRDPKGVIHSHQTLTFETR---QLLAN
MAV_2638|M.avium_104 ARYR-RPAVAADDVCLLLYTSGTTSAPKGVQHTNNTVLAEQRTMPALLAG
MMAR_0303|M.marinum_M ETAAPLPTADPDAVRMVLYTSGTTGRPKGVLHTHNSIHALICQIRDHWMV
Mb3591|M.bovis_AF2122/97 AVAARAAAVAPQDVSDILFTSGTTGRSKGVLCAHRQSLSASASWAANGKI
Rv3561|M.tuberculosis_H37Rv AVAARAAAVAPQDVSDILFTSGTTGRSKGVLCAHRQSLSASASWAANGKI
MUL_4126|M.ulcerans_Agy99 AVTARAAAVSPDDVSDILFTSGTTGRSKGVLCAHRQSLSASASWAANGKI
MAB_4377c|M.abscessus_ATCC_199 ALRARMSQLSNTDPINIQYTSGTTGYPKGATLSHRNVLNNGFFVAESIQL
MLBr_02546|M.leprae_Br4923 SSAP--APKVRKRASIIILTSGTTGTPKGANRNTPASLAPLGGILSKVPF
: ***** .**.
Mflv_2523|M.gilvum_PYR-GCK YPPDRGRQLTATPVGHFIGMLGAFLIPVLEGSPIDLCDV-WDPGRVLQLM
Mvan_4135|M.vanbaalenii_PYR-1 YPPDRGRQLTATPVGHFIGMLGAFLIPVLEGAPIDLCDV-WDPGRVLKLI
MSMEG_4774|M.smegmatis_MC2_155 YPPNRGRQLTATPVGHFIGMVGAFLIPVLEGAPVDLCDV-WDPAKVLRLM
TH_2816|M.thermoresistible__bu YPPDRGRQLTATPVGHFIGMVGAFLIPVLEGAPIDLADV-WDPGRVLRLM
MAV_2638|M.avium_104 KPDD--VSLVTFPPGHIAG-VGSMLRPLLSGARTVFMDG-WDPARAVEVV
MMAR_0303|M.marinum_M EPGD--GFLVASPIAHIGGSIYAFECPLLLGTTAVLLDR-WDADRAVQLM
Mb3591|M.bovis_AF2122/97 TSDD--RYLCINPFFHNFGYKAGILACLQTGATLIPHVT-FDPLHALRAI
Rv3561|M.tuberculosis_H37Rv TSDD--RYLCINPFFHNFGYKAGILACLQTGATLIPHVT-FDPLHALRAI
MUL_4126|M.ulcerans_Agy99 TADD--RHLCINPFFHNFGYKAGILACLQTGATLFPHLT-FDPLRTLQAI
MAB_4377c|M.abscessus_ATCC_199 QAGD--RLCIPVPFYHCFGMVMGNLGCTTHGATIVMPAPGFDPGRTLEAI
MLBr_02546|M.leprae_Br4923 RAHE--VTLLPAPMFHALGYLHATLAMFL-GSTLVLRRR-FKPATVLEDI
. : * * * . *: :.. .:. :
Mflv_2523|M.gilvum_PYR-GCK ETEGLSIGGGPPYFVTSLLD--HPQCRPEHVRRFKTVGLGGSTVPAAVTR
Mvan_4135|M.vanbaalenii_PYR-1 ESDGLSIGGGPPYFVTSLLD--HPDFRPEHLRRFKTVGLGGSTVPAAVTQ
MSMEG_4774|M.smegmatis_MC2_155 ESDGLSIGGGPPYFVTSLLD--HPDFADRHLARIRTVGLGGSTVPAAVTR
TH_2816|M.thermoresistible__bu EAEDISIGGGPPYFVTSLLD--HPDCTPEHLARFKTVGLGGSTVPTTVTR
MAV_2638|M.avium_104 HRFGVTCTAGTPFHLAGILD--LGDTG-DKLASLREFLVGAAPVAEEIGR
MMAR_0303|M.marinum_M TSKRCTHMAGATPFLEQLLA--AAQRAGTRLPDLKFFVCGGASVSPSLIR
Mb3591|M.bovis_AF2122/97 ERHRITVLPGPPTIYQSLLD--HPARKDFDLSSLRFAVTGAATVPVVLVE
Rv3561|M.tuberculosis_H37Rv ERHRITVLPGPPTIYQSLLD--HPARKDFDLSSLRFAVTGAATVPVVLVE
MUL_4126|M.ulcerans_Agy99 ERHRITVLPGPPTIYQTLLD--HPARDDYDLSSLRFAVTGAATVPVVLVE
MAB_4377c|M.abscessus_ATCC_199 ERERCVGVYGVPTMFIAMLA--DPGFAHRDLSTLRTGIMAGSVCPVEVMK
MLBr_02546|M.leprae_Br4923 EKHQATAMVVVPVMLSRILDTLENLETKSDLSSLRVVFVSGSQLGAELAT
. :* : :: ..: :
Mflv_2523|M.gilvum_PYR-GCK RLSDLG--MFVFRSYGSTEHPSITGSR-PSAPEDKRLFTDGDARPGVEIK
Mvan_4135|M.vanbaalenii_PYR-1 RLTDLG--LFVFRSYGSTEHPSITGSR-PDAPEVKRLFTDGNVRPGVEIR
MSMEG_4774|M.smegmatis_MC2_155 RLADLG--IFVFRSYGSTEHPSITGSS-PSAPEGKRLYTDGNPRPGVEIR
TH_2816|M.thermoresistible__bu RLAELG--MFVFRSYGSTEHPSITGSG-PAAPEEKRLYTDGNPRPGVEIR
MAV_2638|M.avium_104 RAAQAG--ISTFRSYGATEHPTVTGEH-EGQPQWARLGTDGKPLPGSEIR
MMAR_0303|M.marinum_M CATDYFDKAVVTRVYGSTEVPVTTIGA-PDSS-AHAAATDGRVG-AADVK
Mb3591|M.bovis_AF2122/97 RMQSELDIDIVLTAYGLTEANGMGTMCRPEDDAVTVATTCGRPFADFELR
Rv3561|M.tuberculosis_H37Rv RMQSELDIDIVLTAYGLTEANGMGTMCRPEDDAVTVATTCGRPFADFELR
MUL_4126|M.ulcerans_Agy99 RMQSELDIDIVLTAYGLTEANGMGTMCRADDDALTVATTCGRPFADFELR
MAB_4377c|M.abscessus_ATCC_199 RCIDEMHMAGVAIAYGMTETSPVSCQTLFDDDLDRRTATVGRAHPHIEIK
MLBr_02546|M.leprae_Br4923 RALEELG-PVIYNMYGSTEIAFATIAG--PKDLQINSATVGPVVKGVKVK
. ** ** * * .::
Mflv_2523|M.gilvum_PYR-GCK LGPD-----------GEIFSRGPD--LCLG---YVDD-DLTAQAFDEDGW
Mvan_4135|M.vanbaalenii_PYR-1 LGED-----------GEIFSRGPD--LCLG---YTDD-ELTARAFDDDGW
MSMEG_4774|M.smegmatis_MC2_155 LGPD-----------GEIFSRGPD--LCLG---YTDD-ALTAKAFDDEGW
TH_2816|M.thermoresistible__bu IAED-----------GEILSRGPD--LCLG---YTDD-ALTARAFDDDGW
MAV_2638|M.avium_104 IVGSD-GTDCPIGIGGEVVVRGPD--QFIG---YQDS-ALNTEAFTDDGW
MMAR_0303|M.marinum_M LVAG------------EIRVRGPQ--MLVG---YRHP-EDEAGSFDAQGY
Mb3591|M.bovis_AF2122/97 IADD-----------GEVLLRGPN--VMVG---YLDDTEATAAAIDADGW
Rv3561|M.tuberculosis_H37Rv IADD-----------GEVLLRGPN--VMVG---YLDDTEATAAAIDADGW
MUL_4126|M.ulcerans_Agy99 IGPDT----------GEVLLRGPN--VMLG---YLDDPRATAAAIDADGW
MAB_4377c|M.abscessus_ATCC_199 IVDPNSGETVQRGQSGELCTRGYS--VMLG---YWNDEAHTREVLDTDGW
MLBr_02546|M.leprae_Br4923 ILDDS----------GKEVPRGQVGRIFVGNAFPFEGYTGGGGKQIIDGL
: : ** :* . :*
Mflv_2523|M.gilvum_PYR-GCK YHTGDIGVLDEDGYLTITDRKADVIIRGGENISALEVEEILLGMPAVAEA
Mvan_4135|M.vanbaalenii_PYR-1 YRTGDVGVLDDDGYLTITDRKADVIIRGGENISALEVEEVLLAMPAVAEA
MSMEG_4774|M.smegmatis_MC2_155 YRTGDVGFLDDDGYLTITDRTADLIIRGGENISALEVEEVLLGIPGVIEA
TH_2816|M.thermoresistible__bu YHTGDIGVLDDDGYLTITDRKADLIIRGGENISALEVEEVLLGMPAVAEA
MAV_2638|M.avium_104 FRTGDLGHLDAHGRLTITDRIKDVIIRGGETISSGQVEEVLNAHPAVADG
MMAR_0303|M.marinum_M FRTGDLGHWVDDEYLVVTGRAKDIIIRNGENISPKEIEDILITHPGIADV
Mb3591|M.bovis_AF2122/97 LHTGDIGAVDQAGNLRINDRLKDMYICGGFNVYPAEVEQVLARMDGVADA
Rv3561|M.tuberculosis_H37Rv LHTGDIGAVDQAGNLRITDRLKDMYICGGFNVYPAEVEQVLARMDGVADA
MUL_4126|M.ulcerans_Agy99 LHTGDIGVLDEAGNLRITDRLKDMYICGGFNVYPAEVEQVLARLDGVADV
MAB_4377c|M.abscessus_ATCC_199 MHTGDLAVMRDDGYCTIIGRLKDMVIRGGENIYPREIEEFLLTHPDIEDV
MLBr_02546|M.leprae_Br4923 LSSGDVGYFDEHDLLYISGRDDEMIVSGGENVFPAEVEDLISGHPEVVEA
:**:. : .* :: : .* .: . ::*:.: : :
Mflv_2523|M.gilvum_PYR-GCK VVVAAPDERLGEHTAAVLRIRDGHVMPSLDDVRAHFRAAGAATQKWPEEL
Mvan_4135|M.vanbaalenii_PYR-1 VVVAAPDARLGEHTAAVLRIREGQSMPALGDVREHFKQAGVATQKWPEEL
MSMEG_4774|M.smegmatis_MC2_155 IVVAAPDARLGERVAAVLRTRSGAPLPTLDDVRAHFERVGIARQKWPEEL
TH_2816|M.thermoresistible__bu VVVAAPDPRLGERTAAVVRVKPGHAMPTLDEVRDHFERTGVARQKWPEEL
MAV_2638|M.avium_104 VAVAAPDSRYGDVVAAVVVLKPGYALN-LDDLRAHFAASGLARQKTPERL
MMAR_0303|M.marinum_M AIVGLPDDRTGERACAVIVAAAD-PPPGLPGVRAFLEGAGVARFKIPEQV
Mb3591|M.bovis_AF2122/97 AVIGVPDQRLGEVGRAFVVARPG-TGLDEASVIAYTREH-LANFKTPRSV
Rv3561|M.tuberculosis_H37Rv AVIGVPDQRLGEVGRAFVVARPG-TGLDEASVIAYTREH-LANFKTPRSV
MUL_4126|M.ulcerans_Agy99 AVIGIPDHRLGEVGRAFIVARPG-FNLDEKSVIDYTREH-LANFKAPRSV
MAB_4377c|M.abscessus_ATCC_199 HVVGVPDEKYGEELCAWVRMRPDRVVIDAVAIRAFASGR-LAHYKIPRYV
MLBr_02546|M.leprae_Br4923 TAIGVDDKEWGARLRAFVVKKQD-ATIDEDAIKAYVRDH-LARYKVPREV
:. * . * * : . : . * * *. :
Mflv_2523|M.gilvum_PYR-GCK HRVPEGQDFPRTASGKVQKYVIRQQVAAGMHGVVAASQK--
Mvan_4135|M.vanbaalenii_PYR-1 HRVPEGQDFPRTASGKVQKFVIRQQLAAGELSAIATGQE--
MSMEG_4774|M.smegmatis_MC2_155 HHVPDGQDFPRTASGKVQKYRVRQAVRNPAAVMDIATSAN-
TH_2816|M.thermoresistible__bu HEVS---DYPRTASGKVQKYRVRQSIAEIAAAQQ-------
MAV_2638|M.avium_104 AIVD---ELPRTSLGKVRKAQLRSDHFGAARR---------
MMAR_0303|M.marinum_M VIWD---HLPKNHAGKIVKHRIKAALMKAEQ----------
Mb3591|M.bovis_AF2122/97 RFVD---VLPRNAAGKVSKPQLRELG---------------
Rv3561|M.tuberculosis_H37Rv RFVD---VLPRNAAGKVSKPQLRELG---------------
MUL_4126|M.ulcerans_Agy99 RFVD---TLPRNAGGKVVKPQLRELA---------------
MAB_4377c|M.abscessus_ATCC_199 HVVE---SFPMTVTGKVRKIEMRQQTIQIFGLPEPGRTDEQ
MLBr_02546|M.leprae_Br4923 IFLE---ELPRNPTGKVLKRELRDFEVH-------------
* . **: * ::