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MTARAAPDTAQTSLAYRRGWWVRSTLADAARAAAQRTPERIALVDNEVRLNCATLYAQAGELAAALLARI PAGSVVSFMLPNWHEAAVIYLAATLAGMVVNPILPSLRDHDLRFVLEDAGAAMVFVPHRYGGHDYPAMLD RVTAAMSAAPQVVVLRGPDTGRTGRHTPYRCMLGGPPGGLPALDPDAVRMILYTSGTTSRPKGVLHTHNS IHALICQIRDHWAIDPGDTFLVPSPLAHIGGSIYAFECPLLLGTTAVLMDRWDPARAVALMTAHRCTHMA GATPFLQQLLSAAANAGTRLPDLKVFICGGASVSPSLIRRAAAYFDRAVVTRVYGCTEVPVATVGAPRPQ EADYAADTDGRPGIAEIKLVAHPAAPTGDGEICVRGPQMLRGYRHPEDDAESFDAAGFFRTGDLGRWALA DSADRYLVVTGRAKDVIIRSGENISAKEVEDLLADHPGIAEIAVVGLPDERTGERACAVIVPTPGASPDV ASLLALLVSKGVAKFKAPEQVVLWDALPKNDAGKVLKHRIRAALSKDG
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. avium 104 | MAV_5212 | - | - | 100% (538) | AMP-binding enzyme, putative |
| M. avium 104 | MAV_2116 | - | 5e-73 | 36.52% (512) | AMP-binding enzyme, putative |
| M. avium 104 | MAV_1957 | - | 3e-66 | 35.56% (540) | AMP-dependent synthetase and ligase |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb2405 | mbtA | 6e-48 | 32.01% (553) | bifunctional salicyl-AMP ligase/salicyl-S-arcp synthetase |
| M. gilvum PYR-GCK | Mflv_1784 | - | 0.0 | 64.73% (516) | AMP-dependent synthetase and ligase |
| M. tuberculosis H37Rv | Rv3561 | fadD3 | 2e-48 | 32.30% (514) | acyl-CoA synthetase |
| M. leprae Br4923 | MLBr_01051 | xclC | 4e-34 | 32.14% (364) | acyl-CoA synthetase |
| M. abscessus ATCC 19977 | MAB_2247c | - | 1e-47 | 31.72% (536) | bifunctional salicyl-AMP ligase/salicyl-S-arcp synthetase |
| M. marinum M | MMAR_0303 | - | 0.0 | 66.42% (533) | acyl-CoA synthetase |
| M. smegmatis MC2 155 | MSMEG_5631 | - | 0.0 | 68.20% (522) | AMP-dependent synthetase and ligase |
| M. thermoresistible (build 8) | TH_3067 | - | 0.0 | 71.10% (526) | PUTATIVE AMP-binding enzyme, putative |
| M. ulcerans Agy99 | MUL_3641 | mbtA | 9e-49 | 31.33% (533) | bifunctional enzyme MbtA: salicyl-AMP ligase (SAL-AMP |
| M. vanbaalenii PYR-1 | Mvan_4966 | - | 0.0 | 68.16% (512) | AMP-dependent synthetase and ligase |
CLUSTAL 2.0.9 multiple sequence alignment
Mb2405|M.bovis_AF2122/97 -MPPKAADGRRPSPDGGLGGFVPFPADRAASYRAAGYWSGRTLDTVLSDA
MUL_3641|M.ulcerans_Agy99 -----------------MAGFIPYPPERVASYRAAGYWTGRSIGSLLRQA
MAB_2247c|M.abscessus_ATCC_199 MTQPRSTTTASSAATPALDGFVPFPSVRADEYRRAGYWTGNTVESLLRVS
Mflv_1784|M.gilvum_PYR-GCK --------------------------------------MTGTLVEALRDA
Mvan_4966|M.vanbaalenii_PYR-1 --------------------------------------MT-TLADALRDA
TH_3067|M.thermoresistible__bu ---------MTETPGAGYRTDRPGHAERAAQAYARGLWVPTTLAEALAEA
MSMEG_5631|M.smegmatis_MC2_155 --------------------------MRAEDAYERGLWVRTTLADTLAEA
MAV_5212|M.avium_104 -----------------MTARAAPDTAQTSLAYRRGWWVRSTLADAARAA
MMAR_0303|M.marinum_M -----MGGPKVPQRRLIDGRWVRWDADRAAGAYAHRWWVRRTLADALREA
Rv3561|M.tuberculosis_H37Rv -----------------------------------MINDLRTVPAALDRL
MLBr_01051|M.leprae_Br4923 -----------------------------------------MLLASLNPS
:
Mb2405|M.bovis_AF2122/97 ARRWPDRLAVADAGDRPGHGGLSYAELDQRADRAAAALHGLGITPGDRVL
MUL_3641|M.ulcerans_Agy99 AARWPDRVGVVDAG-----ASHTFCELDKLADRVAAGFVSLGIAPGDRVL
MAB_2247c|M.abscessus_ATCC_199 AARRPCHPAVIDEH-----RALSYRELDEAADNAAHAFLRLGIAPGDRVL
Mflv_1784|M.gilvum_PYR-GCK ARDTPDRVLVRDDA-----VALTCAELLSAATGLAHALTRR-MPAGSVVS
Mvan_4966|M.vanbaalenii_PYR-1 ARDTPDRILLRDDE-----ASLSSAELLSAATNLAHALVRR-MPVGSVVS
TH_3067|M.thermoresistible__bu ARTTPDRTVLVDGA-----VRLDCRSLHARATALAGALLRR-IPAGAVVS
MSMEG_5631|M.smegmatis_MC2_155 ARDTPQRVLLIDGD-----VRLTCAELHSRAKALAHEMAAR-MPAGSVVS
MAV_5212|M.avium_104 AQRTPERIALVDNE-----VRLNCATLYAQAGELAAALLAR-IPAGSVVS
MMAR_0303|M.marinum_M AQSTPQRVALVDAD-----RQLDCQELHRQAMALAQHLRSR-MPAGSVVS
Rv3561|M.tuberculosis_H37Rv VRQLPDHTALIAED-----RRFTSTELRDAVYGAAAALIALGVEPADRVA
MLBr_01051|M.leprae_Br4923 VVTATDIADAVCVDG---------VVLSRSGLVGAATSVAERVGGARLVA
. . * * : . *
Mb2405|M.bovis_AF2122/97 LQLPNGCQFAVALFALLRAGAIPVMCLPGHRAAELGHFAAVSAATGLVVA
MUL_3641|M.ulcerans_Agy99 LQLPNSCRFAVALFGLLRAGAIPVMCLPGHRLAELHHFAEVSGAVGLIVA
MAB_2247c|M.abscessus_ATCC_199 LQLPNRASFAVALFGLMRAGAIPVMCLPGHRAAELSHFIEVADAVGLVIP
Mflv_1784|M.gilvum_PYR-GCK FTVPNWHEAAIIYLGATLAGMVVNPILPSLRERELSFILDDADSRMIFVP
Mvan_4966|M.vanbaalenii_PYR-1 FAVPNWHEAAVIYLGATLAGMVVNPILPSLRERELSFILADAGSRAIFIP
TH_3067|M.thermoresistible__bu FMLPNWHEAAVIYLGATLAGMVVNPILPSLRDRELRFILDDADSRMIFIP
MSMEG_5631|M.smegmatis_MC2_155 FMLPNWHEAAIVYLASTLAGMVINPILPSLRDHELRFILDDAASRMIFIP
MAV_5212|M.avium_104 FMLPNWHEAAVIYLAATLAGMVVNPILPSLRDHDLRFVLEDAGAAMVFVP
MMAR_0303|M.marinum_M FMLPNWHEAAVIYLASTLAAMVANPVLPSLREHELRFILEDAGSRMIFAP
Rv3561|M.tuberculosis_H37Rv IWSPNTWHWVVACLAIHHAGAAVVPLNTRYTATEATDILDRAGAPVLFAA
MLBr_01051|M.leprae_Br4923 VLATPTASTVLAITGCLIAGVPVVPVPADIGVVERRHMLTDSGAQAWLGP
. . .: . *. . : . : : . .
Mb2405|M.bovis_AF2122/97 DVASGFDYRPMARELVADHPTLRHVIVDGDPGP-----FVSWAQLCAQAG
MUL_3641|M.ulcerans_Agy99 QHAGGFDYRSMAERLVAAHPQLRHVIIDGESGP-----FLPWSSLSEGA-
MAB_2247c|M.abscessus_ATCC_199 DSAGGFDYRALAVALSQRHPRLRTVVVEGDAGP-----FASWTSLLKSVE
Mflv_1784|M.gilvum_PYR-GCK ESFGGHDYAAMLDRVAPGLPSPPEVVVVRGE------GRVTLADLLAEPA
Mvan_4966|M.vanbaalenii_PYR-1 QTFGGHDYAAMLDRVVAGLPSPPEVVVVRGEP-----RRPALTDLVSEPR
TH_3067|M.thermoresistible__bu ETFGNHDYAAMLDRVTAELPNPPQVVVVRGAVR---PDRVSYDALLSGPV
MSMEG_5631|M.smegmatis_MC2_155 GEFGQHDYAAMLQRVCAHLPTPPQVVVVRGEP-----GPHTAFTALGAPG
MAV_5212|M.avium_104 HRYGGHDYPAMLDRVTAAMSAAPQVVVLRGPDTGRTGRHTPYRCMLGGPP
MMAR_0303|M.marinum_M ADFRGHDYTSMLRRVAAQLPSRPEVIEVRGQCP---DGVSTFQSLLVGRE
Rv3561|M.tuberculosis_H37Rv GLFLGADRAAGLDR--AALPALRHVVRVPVEADDGTWDEFIATGAGALDA
MLBr_01051|M.leprae_Br4923 GPS--------ADSAPDGLPHIPVQLDARSWNR-----------------
. :
Mb2405|M.bovis_AF2122/97 TGSPAPPADPGSPALLLVSGGTTGMPKLIPRTHDDYVFNATASAALCRLS
MUL_3641|M.ulcerans_Agy99 --TPDIDVDTTSPALLLVSGGTTGTPKLIPRTHDDYVYNATASAQLCRLT
MAB_2247c|M.abscessus_ATCC_199 PQGPLPTVQTDTPALLLVSGGTTAAPKLIPRTHEDYVYNATQAARVCGLT
Mflv_1784|M.gilvum_PYR-GCK DG-VLPAPRPTDTRMILYTSGTTGRPKGVLHTHESLAALIAQLGRFWRID
Mvan_4966|M.vanbaalenii_PYR-1 DG-TLPDLDPAATRMILYTSGTTGRPKGVLHSHKSLGALIAQLGRYWRID
TH_3067|M.thermoresistible__bu DRPVLPALDPDAVRMILYTSGTTGRPKGVLHSHNSIHALIHQLREHWLID
MSMEG_5631|M.smegmatis_MC2_155 GD--LPALDPDSVRMMMHTSGTTGRPKGVLHSHNSIHALICQLRENWMID
MAV_5212|M.avium_104 GG--LPALDPDAVRMILYTSGTTSRPKGVLHTHNSIHALICQIRDHWAID
MMAR_0303|M.marinum_M TAAPLPTADPDAVRMVLYTSGTTGRPKGVLHTHNSIHALICQIRDHWMVE
Rv3561|M.tuberculosis_H37Rv VAARAAAVAPQDVSDILFTSGTTGRSKGVLCAHRQSLSASASWAANGKIT
MLBr_01051|M.leprae_Br4923 ----YPEPSPDATAMVIYTSGTTGPPKGVLLSRRAIAVDLDALAQAWQWT
. :: :.***. .* : ::
Mb2405|M.bovis_AF2122/97 ADDVYLVVLAAGHN----FPLACPGLLGAMTVGATAVFAPDPSPEAAFAA
MUL_3641|M.ulcerans_Agy99 ADDVYLVSLPAAHN----FPLACPGILGSMAVGATIVFSTDPSPEAAFTL
MAB_2247c|M.abscessus_ATCC_199 QDDVYLVALPAGHN----FPLACPGLLGAISTGATTVFLSDPSPESAFAT
Mflv_1784|M.gilvum_PYR-GCK RGDTFLVPSPIAHIGGSIYAFECP-----LLLGTEAVLMQRWDPDAAVGL
Mvan_4966|M.vanbaalenii_PYR-1 PGDTFLVPSPIAHIGGSIYAFECP-----LLLGTQAVLMQRWDPDAAVAL
TH_3067|M.thermoresistible__bu PGDTFLVPSPIAHIGGSIYAFECP-----LLAGTTAVLMDRWRPDEAVRL
MSMEG_5631|M.smegmatis_MC2_155 PGDTFLVPSPIAHIGGSIYAFECP-----LLLGTTAVLMQRWNADDGVAL
MAV_5212|M.avium_104 PGDTFLVPSPLAHIGGSIYAFECP-----LLLGTTAVLMDRWDPARAVAL
MMAR_0303|M.marinum_M PGDGFLVASPIAHIGGSIYAFECP-----LLLGTTAVLLDRWDADRAVQL
Rv3561|M.tuberculosis_H37Rv SDDRYLCINPFFHN----FGYKAG-ILACLQTGATLIPHVTFDPLHALRA
MLBr_01051|M.leprae_Br4923 ADDVLVHGLPLFHVHGLVLGLLGS-----LRIGNRFVHTGKPTPTAYAQA
.* : . * : * : .
Mb2405|M.bovis_AF2122/97 IERHGVTVTALVPALAKLWAQSCEWEPVTPKSLRLLQVGGSKLEPEDARR
MUL_3641|M.ulcerans_Agy99 IERHGVTVTALVPALAKLWTQACDWEPVIPKSLRLLQVGGSRLEPEDARQ
MAB_2247c|M.abscessus_ATCC_199 IARHRVSVTAVVPSLAQLWAQATAWEPVLPETLRLLQVGGAKLGADDARA
Mflv_1784|M.gilvum_PYR-GCK MLEHRVTHMAGATPFLVGLLAAAQRAGTRLPDLKVFICGGASVPPTLIRS
Mvan_4966|M.vanbaalenii_PYR-1 MLRHRCTHMAGATPFLDGLLAAAERAGTRLPDLKVFICGGASVPPSLIRR
TH_3067|M.thermoresistible__bu MRAEHCTHMAGATPFLDGLLTAARQAGTRLPDLKVFICGGASVSPSLIRS
MSMEG_5631|M.smegmatis_MC2_155 MSEHHCTHMAGATPFLDQLLNAAERAGTRLPDLKVFICGGASVPPSLIRR
MAV_5212|M.avium_104 MTAHRCTHMAGATPFLQQLLSAAANAGTRLPDLKVFICGGASVSPSLIRR
MMAR_0303|M.marinum_M MTSKRCTHMAGATPFLEQLLAAAQRAGTRLPDLKFFVCGGASVSPSLIRC
Rv3561|M.tuberculosis_H37Rv IERHRITVLPGPPTIYQSLLDHPARKDFDLSSLRFAVTGAATVPVVLVER
MLBr_01051|M.leprae_Br4923 CSEAGGSLYFGVPTVWSRLVADEAVARALRP-ARLLVSGSASLPVPVFDR
: ... :. *.: :
Mb2405|M.bovis_AF2122/97 VRTALTPG-LQQVFGMAEGL-LNFTRIGDPPEVVEHTQGRPLCPADELRI
MUL_3641|M.ulcerans_Agy99 AREALTPG-LQQVFGMAEGL-LNFTRLEDPPDLLEHTQGRPLCSADELRI
MAB_2247c|M.abscessus_ATCC_199 VRESLTQG-LQQVFGMAEGL-LCLTQPGDPTDILDNSQGRPMCDADEVRI
Mflv_1784|M.gilvum_PYR-GCK AAAYFDKAAVSRVYGSTEVP-VTTVGALD--DIDRAADTDGRPGIAEVRI
Mvan_4966|M.vanbaalenii_PYR-1 AADYFDKAAVSRVYGSTEVP-VTTVGSLD--DVDRAADTDGRPGIADVTL
TH_3067|M.thermoresistible__bu AADYFERAVVTRVYGSTEVP-VTTVGVLGPADADRAAETDGRPGIAEVRL
MSMEG_5631|M.smegmatis_MC2_155 ASTCFEKAVVSRVYGSTEVP-VTTVGSIEPGDVEHAACTDGRPGLAEIRL
MAV_5212|M.avium_104 AAAYFDRAVVTRVYGCTEVP-VATVGAPRPQEADYAADTDGRPGIAEIKL
MMAR_0303|M.marinum_M ATDYFDKAVVTRVYGSTEVP-VTTIGAPD--SSAHAAATDGRVGAADVKL
Rv3561|M.tuberculosis_H37Rv MQSELDIDIVLTAYGLTEANGMGTMCRPEDDAVTVATTCGRPFADFELRI
MLBr_01051|M.leprae_Br4923 LAHLTGHRPIERYGSTESLITLSTLADGE----RRAGWVGLPLAGVQTRL
: . . : : :
Mb2405|M.bovis_AF2122/97 VNADGEPVGPG--EEGELLVRGPYTLNGYFAAERDNERCFDPDGFYRSGD
MUL_3641|M.ulcerans_Agy99 VDGAGAPVAPG--AEGELLVRGPYTINGYFCADRDNERCFDPAGFYRSGD
MAB_2247c|M.abscessus_ATCC_199 VDEDGADVPAG--ETGELLVRGPYTLNGYYRADADNMRSFTVDGFYRSGD
Mflv_1784|M.gilvum_PYR-GCK VD-------------GEIRARGPQMFTGYLHP-EDDRESFDDAGYFRTGD
Mvan_4966|M.vanbaalenii_PYR-1 VD-------------GEIRARGPQMLTGYLHP-EDNSESFDAEGFFRTGD
TH_3067|M.thermoresistible__bu ADHAAAPPG-----AGEVLARGPQMLVGYLHP-EDERDAFDADGFFRTGD
MSMEG_5631|M.smegmatis_MC2_155 VD-------------GEIRARGPQMLLGYLHA-EDN--AFDEQGFFRTGD
MAV_5212|M.avium_104 VAHPAAPTG-----DGEICVRGPQMLRGYRHP-EDDAESFDAAGFFRTGD
MMAR_0303|M.marinum_M VA-------------GEIRVRGPQMLVGYRHP-EDEAGSFDAQGYFRTGD
Rv3561|M.tuberculosis_H37Rv AD------------DGEVLLRGPNVMVGYLDDTEATAAAIDADGWLHTGD
MLBr_01051|M.leprae_Br4923 VDESGGPVPYDGETVGRLQVRSPTMFGGYLNRPEATAEAFDEDGWYRTGD
. *.: *.* : ** .: *: ::**
Mb2405|M.bovis_AF2122/97 LVRRRDDG----NLVVTGRVKDVICRAGETIAASDLEEQLLSHPAIFSAA
MUL_3641|M.ulcerans_Agy99 LVRLRDDG----YLQVTGRVKDVICRCGETISAQDLEEQLLSHPAIRSAA
MAB_2247c|M.abscessus_ATCC_199 RVRALPGG----YLEVTGRIKDVILRGGESIAALDLESHLQTHPAVYAAA
Mflv_1784|M.gilvum_PYR-GCK LGRFTEDN----HLVVTGRAKDIIIRNGENISPKEVEDILVTHPRIAEIA
Mvan_4966|M.vanbaalenii_PYR-1 LGHLTDDG----YLVVTGRAKDLIIRNGENISPKEVEDILVTHPQIAEIA
TH_3067|M.thermoresistible__bu LARWVDGD----HLVITGRAKDIIIRNGENISPKEVEDLLIGHPGVAEIA
MSMEG_5631|M.smegmatis_MC2_155 LGRWVDGD----YLTVTGRAKDLIIRNGENISPKEIEDILVGELGINEIA
MAV_5212|M.avium_104 LGRWALADSADRYLVVTGRAKDVIIRSGENISAKEVEDLLADHPGIAEIA
MMAR_0303|M.marinum_M LGHWVDDE----YLVVTGRAKDIIIRNGENISPKEIEDILITHPGIADVA
Rv3561|M.tuberculosis_H37Rv IGAVDQAG----NLRITDRLKDMYICGGFNVYPAEVEQVLARMDGVADAA
MLBr_01051|M.leprae_Br4923 VAVVDSGG---MHRIVGRESVDLIKLGGYRIGAGEIEMALLGHPDVREVA
: . *: * : . ::* * : *
Mb2405|M.bovis_AF2122/97 AVGLPDQYLGEKICAAVVFAG---APITLAELNGYLDRRGVAAHTRPDQL
MUL_3641|M.ulcerans_Agy99 AVPLPDPDMGELICAAIVFAGPPQTPQTLSGLNDYLEQRGVAAHIRIDRL
MAB_2247c|M.abscessus_ATCC_199 AVGLPDQYLGEIVCAAIVFKG---KPVAAAELNQHLQARGAATHSRVDKL
Mflv_1784|M.gilvum_PYR-GCK VVGIPDPRTGERACAALVTTE--DPPPDIAELKDFLTDRGVARFKVPEQI
Mvan_4966|M.vanbaalenii_PYR-1 IVGIPDLRTGERACAVIVAAD--SAAPDVAEIRDFLVARGVAKFKAPERV
TH_3067|M.thermoresistible__bu VVGVPDPRTGERACAAIVPTG--RPGPDVAELREFLLARGVAKFKAPEQV
MSMEG_5631|M.smegmatis_MC2_155 VVGLPDERTGERACAVVVTED--GARPDVSALAEVLTKAGVAKFKIPEQV
MAV_5212|M.avium_104 VVGLPDERTGERACAVIVPTP--GASPDVASLLALLVSKGVAKFKAPEQV
MMAR_0303|M.marinum_M IVGLPDDRTGERACAVIVAAA--DPPPGLPGVRAFLEGAGVARFKIPEQV
Rv3561|M.tuberculosis_H37Rv VIGVPDQRLGEVGRAFVVARP--GTGLDEASVIAYTR-EHLANFKTPRSV
MLBr_01051|M.leprae_Br4923 VVGLPDEDLGQRIVAFVVGAE----ALDADELINYVA-QQLSIHKRPREV
: :** *: * :* : : . :
Mb2405|M.bovis_AF2122/97 VAMPALPTTPIGKIDKRAIVRQLGIATGPVTTQRCH
MUL_3641|M.ulcerans_Agy99 VAMPSLPTTPVGKIDKKAIASQILTTTAT-------
MAB_2247c|M.abscessus_ATCC_199 VAVSALPLTAVGKIDKRALLASLTG-----------
Mflv_1784|M.gilvum_PYR-GCK HVVGALPKNDAGKVLKHVLKAELTKES---------
Mvan_4966|M.vanbaalenii_PYR-1 VVVDALPKNDAGKVLKHAIRAVLLEKPREGEP----
TH_3067|M.thermoresistible__bu VFFDALPKNDAGKVLKHQIQATLTAAEVSG------
MSMEG_5631|M.smegmatis_MC2_155 EIWDALPKNDAGKVLKHQIRTQLSKVE---------
MAV_5212|M.avium_104 VLWDALPKNDAGKVLKHRIRAALSKDG---------
MMAR_0303|M.marinum_M VIWDHLPKNHAGKIVKHRIKAALMKAEQ--------
Rv3561|M.tuberculosis_H37Rv RFVDVLPRNAAGKVSKPQLRELG-------------
MLBr_01051|M.leprae_Br4923 RFVDALPRNAMGKVLKKQLLFEG-------------
** . **: * :