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NLFALLEQAAARHGERGAVYHGVQQIHSWASLRERALRLAGSLRDLGPGARIAVASENRPEIVELMFAIW AAECVFVPLNFKLHPREMEQILDDSGAARVFASPKIAAQLTPVTSAPVEVTGSAEYRSRCAADPLSVPRY TEAASLAWLFYTSGTTGRSKGAMLSHRNLMAMTVSHLADFDSPDENSSLIHGAPMSHGSGLYVPPYVSRG ARQVVPASGAFDPDEFLDLCGQHPRCSAFLAPTMVARLVQTGRPSPAHLDTVVYGGGPMYVDSLKKAMAA FGPIFVQLYGQGEAPMTITGLRRGDHVTADGWAPDAVLGSVGYARSGVDVAVLGEDGEPAGVDEIGEIVC RGDVVMSGYWNNLGATQATLKDGWLRTGDMGSFDAHGFLTLRDRSKDVVISGGSNIYPREVEEVLLEHPG VLEVGVVGAPDTDWGEVVVAFVVGDVGVAELDAHLLERIARFKRPKRYEFVDELPKNSYGKVLKRELRER LK*
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. gilvum PYR-GCK | Mflv_1782 | - | - | 100% (493) | AMP-dependent synthetase and ligase |
| M. gilvum PYR-GCK | Mflv_0027 | - | 8e-68 | 37.14% (482) | AMP-dependent synthetase and ligase |
| M. gilvum PYR-GCK | Mflv_4616 | - | 3e-62 | 33.53% (504) | AMP-dependent synthetase and ligase |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb0172 | fadD5 | 2e-62 | 34.02% (488) | acyl-CoA synthetase |
| M. tuberculosis H37Rv | Rv0166 | fadD5 | 2e-62 | 34.22% (488) | acyl-CoA synthetase |
| M. leprae Br4923 | MLBr_01051 | xclC | 8e-40 | 31.32% (463) | acyl-CoA synthetase |
| M. abscessus ATCC 19977 | MAB_4569c | - | 6e-63 | 36.05% (491) | fatty-acid-CoA ligase FadD |
| M. marinum M | MMAR_0409 | fadD5 | 5e-63 | 34.29% (490) | fatty-acid-CoA ligase FadD5 |
| M. avium 104 | MAV_3523 | - | 9e-65 | 35.24% (508) | acyl-CoA synthase |
| M. smegmatis MC2 155 | MSMEG_5633 | - | 0.0 | 75.56% (495) | feruloyl-CoA synthetase |
| M. thermoresistible (build 8) | TH_3645 | - | 0.0 | 75.71% (494) | PUTATIVE AMP-dependent synthetase and ligase |
| M. ulcerans Agy99 | MUL_0650 | fadD8 | 1e-61 | 33.27% (520) | acyl-CoA synthetase |
| M. vanbaalenii PYR-1 | Mvan_4968 | - | 0.0 | 84.35% (492) | AMP-dependent synthetase and ligase |
CLUSTAL 2.0.9 multiple sequence alignment
Mflv_1782|M.gilvum_PYR-GCK -------------------------MNLFALLEQAAARHGERGAVYHGVQ
Mvan_4968|M.vanbaalenii_PYR-1 -------------------------MNLFALLDQTAARHGDRGAVYHGER
TH_3645|M.thermoresistible__bu ---------------------MGAAVNLFALLDQTATRFGDRGAVFHGER
MSMEG_5633|M.smegmatis_MC2_155 -------------------------MNLFGLLDQTAARHGDRGAVFCGER
MLBr_01051|M.leprae_Br4923 ------------------MLLASLNPSVVTATDIADAVCVDGVVLSRSG-
MAV_3523|M.avium_104 ------------------MPAAETRETLAGIVERHAQRRPDAIAIRYGER
Mb0172|M.bovis_AF2122/97 MTAQLASHLTRALTLAQQQPYLARRQNWVNQLERHAMMQPDAPALRFVGN
Rv0166|M.tuberculosis_H37Rv MTAQLASHLTRALTLAQQQPYLARRQNWVNQLERHAMMQPDAPALRFVGN
MMAR_0409|M.marinum_M MTAELASHLPQAA-HALEQPYLARRQNWVNQLERHAMMQPRATAIRYLGH
MAB_4569c|M.abscessus_ATCC_199 MTDTIDATVQSAG---LAQPYRARRQNWCNQLARHALMQPDATAIRYLGH
MUL_0650|M.ulcerans_Agy99 ----------------MSDDLLRNPTHNGHLLVGALERHKNRPVLFLGDT
.:
Mflv_1782|M.gilvum_PYR-GCK QIHSWASLRERALRLAGSLR-DLGPGARIAVASENRPEIVELMFAIWAAE
Mvan_4968|M.vanbaalenii_PYR-1 LVHTWSSLRERALRLASSLR-EFGPGARIAVASENRPEIVELMFAIWAAE
TH_3645|M.thermoresistible__bu QVTTWSGLRDRALRLAGSIRREVDAGARIAIASENRPEIVELMFAVWAAE
MSMEG_5633|M.smegmatis_MC2_155 ELYTWTQLRDRAARLGSTLT---EPGTRVAVASENRPEIIEIMFGVWAAE
MLBr_01051|M.leprae_Br4923 -----------LVGAATSVAERVGGARLVAVLATPTASTVLAITGCLIAG
MAV_3523|M.avium_104 QWSWAEWSSRIRRAAGALRGDGIERGQCVAFLDKNHPACLEVLIGGASVG
Mb0172|M.bovis_AF2122/97 TMTWADLRRRVAALAGALSGRGVGFGDRVMILMLNRTEFVESVLAANMIG
Rv0166|M.tuberculosis_H37Rv TMTWADLRRRVAALAGALSGRGVGFGDRVMILMLNRTEFVESVLAANMIG
MMAR_0409|M.marinum_M TVTWADLRYRVAALAGSLSRRGVGFGDRVMILMLNRTEFVESVLAANMLG
MAB_4569c|M.abscessus_ATCC_199 SISWAELHQRVQALADALSRRGVGFGDRVLILMLNRPEYVESWLAINELG
MUL_0650|M.ulcerans_Agy99 TLTGGQLAERISQYIQAFEALGAGTGVTVGLLSLNRPEVLMILGASQARG
. : . . : .
Mflv_1782|M.gilvum_PYR-GCK CVFVPLNFKLHPREMEQILDDSGAARVFASPK--IAAQLTPVTSAPVEVT
Mvan_4968|M.vanbaalenii_PYR-1 CVFVPLNYKLHVREMEQILSDAGAARVFTSPK--IGAELAPVASTGIEII
TH_3645|M.thermoresistible__bu CVVVPINFKLHPREMAQILDDAEASRVFTSAS--IGAALAPATEVPVETV
MSMEG_5633|M.smegmatis_MC2_155 CVYVPINYKLHPREMADILADSGAALVFASPK--IAPGLFEVTDVPVEVV
MLBr_01051|M.leprae_Br4923 VPVVPVPADIGVVERRHMLTDSGAQAWLG-----PGPSADSAPDGLP---
MAV_3523|M.avium_104 AVTTVVNWRVIGDELVHVLADSGARVLVVGAE--LRPAAEAAARRVPSLE
Mb0172|M.bovis_AF2122/97 AIAVPLNFRLTPTEIAVLVEDCAAHVMLTEAA--LAPVAIGVRNIQPLLS
Rv0166|M.tuberculosis_H37Rv AIAVPLNFRLTPTEIAVLVEDCVAHVMLTEAA--LAPVAIGVRNIQPLLS
MMAR_0409|M.marinum_M AIAVPLNFRLTPSEIAFLVEDCAPRLVITEEV--LAQVAVGVREIAPALS
MAB_4569c|M.abscessus_ATCC_199 AIAIPVNFRMTPPEVAFLVENSGAKVAITETV--LAPVAAAVRQQAPALE
MUL_0650|M.ulcerans_Agy99 YRRTALHPLGSLDDHAYVLYDAGASALIIDPNPMFVERALGLLEKVDSLE
: : :: :. . .
Mflv_1782|M.gilvum_PYR-GCK GSAEYRS------------RCAAD----PLSVPRYTEAASLAWLFYTSGT
Mvan_4968|M.vanbaalenii_PYR-1 GAAEYES------------RCAAM----PSPAPRDTDPASPAWLFYTSGT
TH_3645|M.thermoresistible__bu VGDEYAR------------RLTAD----PAPVPTGTDPAALAWLFYTSGT
MSMEG_5633|M.smegmatis_MC2_155 GTDAYTQ------------RFESE----PVPAP-LTDPAELAWLFYTSGT
MLBr_01051|M.leprae_Br4923 -HIPVQ-------LDARSWNRYPE----PSP-------DATAMVIYTSGT
MAV_3523|M.avium_104 RIIEVG-------DEYESLLAAAE----PAPSDAGVDTDETALVIYSSGT
Mb0172|M.bovis_AF2122/97 VIVVAGGSSQDSVFGYEDLLNEAG----DVHEPVDIPNDSPALIMYTAGT
Rv0166|M.tuberculosis_H37Rv VIVVAGGSSQDSVFGYEDLLNEAG----DVHEPVDIPNDSPALIMYTSGT
MMAR_0409|M.marinum_M TIVVAGGASDPTVVGYDELISETG----DPPEPVDIPNDSPALIMYTSGT
MAB_4569c|M.abscessus_ATCC_199 TVIVAGSASENGVLGYEEVIAEPG----EPHAEVDLPGDTPALIMYTSGT
MUL_0650|M.ulcerans_Agy99 QVLTIGPVPEALEDRAVDLSAEAAKYQPKPLVVADLPPNHIGGLTYTGGT
. . : *:.**
Mflv_1782|M.gilvum_PYR-GCK TGRSKGAMLSHRNLMAMTVSHLADFDSPDENSSLIHGAPMSHGSGL-YVP
Mvan_4968|M.vanbaalenii_PYR-1 TGRSKGAMLSHRNLMAMTVSHLADFDCPDENSSLIHGAPMSHGSGL-YVP
TH_3645|M.thermoresistible__bu TGRSKGAMLSHRNLMAMTVAHLADFDDPDQNCSLIHGAPMSHGSGL-YIP
MSMEG_5633|M.smegmatis_MC2_155 TGKSKGAMLSHRNLMAMTVAHLADFDAPDEFCSLVHGAPMSHGSGL-YIP
MLBr_01051|M.leprae_Br4923 TGPPKGVLLSRR-AIAVDLDALAQAWQWTADDVLVHGLPLFHVHGLVLGL
MAV_3523|M.avium_104 TGRPKGVLLSQR-ALVNHAANLAPAFPFGDGDANLVAMPLFHVGGIGYAL
Mb0172|M.bovis_AF2122/97 TGRPKGAVLTHANLTGQAMTALYTSGANINSDVGFVGVPLFHIAGIGNML
Rv0166|M.tuberculosis_H37Rv TGRPKGAVLTHANLTGQAMTALYTSGANINSDVGFVGVPLFHIAGIGNML
MMAR_0409|M.marinum_M TGRPKGAVLTHTNLTGQVMTALYTGGANINSDVGFIGVPFFHIAGIGNML
MAB_4569c|M.abscessus_ATCC_199 TGRPKGAVLTHLNLSGQAMTYLLSTTVDLNSDVGFIGVPMFHIAGVGNMI
MUL_0650|M.ulcerans_Agy99 TGKPKGVIGTAGNITAMTSIQLAEWEWP-EHPRFLMCTPLSHAGAAFFTP
** .**.: : * . *: * .
Mflv_1782|M.gilvum_PYR-GCK PYVSRGARQVVPASGAFDPDEFLDLCGQHPRCSAFLAPTMVARLVQTGR-
Mvan_4968|M.vanbaalenii_PYR-1 PYVSRGARQVVPASGTFDPDEFLDLCESHPGCSAFLAPTMVARLVQTGR-
TH_3645|M.thermoresistible__bu PYVARGARQVIPASGVFDPGEFLDLCEHHPGCSAFLAPTMVQRLIDTGR-
MSMEG_5633|M.smegmatis_MC2_155 PYVLRGARQVIPESGAFEPDEFLDLCDHHPGCSAFLAPTMVQRLIQTGR-
MLBr_01051|M.leprae_Br4923 LGSLRIGNRFVHTG-KPTPTAYAQACSEAGGSLYFGVPTVWSRLVADEA-
MAV_3523|M.avium_104 FG-IRAGAPTIMTR-EPDAAALIGAVRAGATHAFFVPPVIARFLDAGEA-
Mb0172|M.bovis_AF2122/97 TGLLLGLPTVIYPLGAFDPGQLLDVLEAEKVTGIFLVPAQWQAVCTEQQ-
Rv0166|M.tuberculosis_H37Rv TGLLLGLPTVIYPLGAFDPGQLLDVLEAEKVTGIFLVPAQWQAVCTEQQ-
MMAR_0409|M.marinum_M SGMLLGVPTVIYPLGAFNPGQLLDVLEAERVSGIFLVPAQWQAVCAEQQ-
MAB_4569c|M.abscessus_ATCC_199 TGLLLGLPTVIHPLGAFDPGALLDVLESEGVTGIFLVPAQWQAVCAAQQ-
MUL_0650|M.ulcerans_Agy99 TLVKGGE---MIVLAKFDPGEVLRVIEEQRITATMLVPSMLYALMDHPDP
: . : * :
Mflv_1782|M.gilvum_PYR-GCK PSP--AHLDTVVYGGGPMYVDSLKKAMAAFG-PIFVQLYGQGEAPMTITG
Mvan_4968|M.vanbaalenii_PYR-1 ACP--ANLRTIVYGGGPMYVDSLKKAMAAFG-PVFVQLYGQGEAPMTITG
TH_3645|M.thermoresistible__bu SAP--ANLRTIVYGGGPMYVESLKKALAAFG-PVFVQLYGQGEAPMTITG
MSMEG_5633|M.smegmatis_MC2_155 PRP--ANLRTVVYGGGPMYVDSLKKALAAYG-QIFVQLYGQGEAPMTITG
MLBr_01051|M.leprae_Br4923 VARALRPARLLVSGSASLPVPVFDR-LAHLTGHRPIERYGSTESLITLST
MAV_3523|M.avium_104 ARASIAGLRYIVYGAAPMPLPLLHRALSTWPGTKFVQVYGQTELCGAVTA
Mb0172|M.bovis_AF2122/97 ARPRDLRLRVLSWGAAPAPDALLRQMSATFPETQILAAFGQTEMSPVTCM
Rv0166|M.tuberculosis_H37Rv ARPRDLRLRVLSWGAAPAPDALLRQMSATFPETQILAAFGQTEMSPVTCM
MMAR_0409|M.marinum_M ARPRELSLRVMSWGAAPAPDALLRQMSEVFPGTQIMAAFGQTEMSPVTCM
MAB_4569c|M.abscessus_ATCC_199 ANPRKVRLKTLSWGAAPATDVLLRTMSETFPDARILAAFGQTEMSPVTCM
MUL_0650|M.ulcerans_Agy99 HTRDLSSLETVYYGASAINPVRLAEAIDRFG-PIFAQYYGQSEAPMVITY
: *... : :*. * .
Mflv_1782|M.gilvum_PYR-GCK LRRGDHVTAD-GWAPDAVLGSVGYARSGVDVAVLGEDGEPAG--VDEIGE
Mvan_4968|M.vanbaalenii_PYR-1 LRRRDHLDA-----PDAVLGSVGYARSGVDVAVLGPDGAPVA--VGEIGE
TH_3645|M.thermoresistible__bu LRRADHFDETGAVAEDAVLGSVGHPRSGVQVAVLRPDGRPAA--VGEVGE
MSMEG_5633|M.smegmatis_MC2_155 LRRDDHLDAD-----DATLGSVGYPRSGVQVAVLRSDGTPAD--IGEIGE
MLBr_01051|M.leprae_Br4923 LADGERRAG-----------WVGLPLAGVQTRLVDESGGPVPYDGETVGR
MAV_3523|M.avium_104 LSDDDHRDAARP----QLQLSAGKAVQGCEIRIVDPNNCAELPAGR-SGE
Mb0172|M.bovis_AF2122/97 LLGEDAIAKRG---------SVGRVIPTVAARVVDQNMNDVP--VGEVGE
Rv0166|M.tuberculosis_H37Rv LLGEDAIAKRG---------SVGRVIPTVAARVVDQNMNDVP--VGEVGE
MMAR_0409|M.marinum_M LLGEDAIRKRG---------SVGKVIPTVSARVVDDEMNDVP--IGQVGE
MAB_4569c|M.abscessus_ATCC_199 LMGDDAIRKMG---------SVGRVIPTVSARVVDDNMNDVP--VGEVGE
MUL_0650|M.ulcerans_Agy99 LPKADHDEKRLT--------SCGRPTLFARVALLGEDGKPVP--QGEPGE
* : * :: . *.
Mflv_1782|M.gilvum_PYR-GCK IVCRGDVVMSGYWNNLGATQATLKD-GWLRTGDMGSFDAHGFLTLRDR-S
Mvan_4968|M.vanbaalenii_PYR-1 IVCRGDVVMSGYWQNPEATEAALQH-GWLRTGDMGSFDAHGFLTLRDR-S
TH_3645|M.thermoresistible__bu IVCRGDVVMSGYWRNPTATADTLRD-GWLYTGDMGSFDDRGHLTLRDR-S
MSMEG_5633|M.smegmatis_MC2_155 IVCRGDVVMSGYWNNPEATAATLKD-GWLHTGDMGSFDARGHLTLRDR-S
MLBr_01051|M.leprae_Br4923 LQVRSPTMFGGYLNRPEATAEAFDEDGWYRTGDVAVVDSGGMHRIVGRES
MAV_3523|M.avium_104 VWVRSNQNMSGYLNRAEATAETITADGWVRTGDVGRLDADGYVYIEDR-L
Mb0172|M.bovis_AF2122/97 IVYRAPTLMSCYWNNPEATAEAFAG-GWFHSGDLVRMDSDGYVWVVDR-K
Rv0166|M.tuberculosis_H37Rv IVYRAPTLMSCYWNNPEATAEAFAG-GWFHSGDLVRMDSDGYVWVVDR-K
MMAR_0409|M.marinum_M IVYRAPTLMSGYWNNPDATAEAFAG-GWFHSGDLVRMDEDGYVWVVDR-K
MAB_4569c|M.abscessus_ATCC_199 IVYRAPTLMQGYWNNPEATADAFAG-GWFHSGDLVRQDEDGFVWVVDR-K
MUL_0650|M.ulcerans_Agy99 ICVSGPLLAGGYWNKPDDTAQTFRD-GWLHTGDMAREDEDGFYFIVDR-V
: . * .. * :: ** :**: * * : .*
Mflv_1782|M.gilvum_PYR-GCK KDVVISGGSNIYPREVEEVLLEHPGVLEVGVVGAPDTDWGEVVVAFVVG-
Mvan_4968|M.vanbaalenii_PYR-1 KDVVISGGSNIYPREVEEVLLEHPGVVEAGVVGAPDEEWGEVVVAFIVG-
TH_3645|M.thermoresistible__bu KDVVISGGSNIYPREVEEVLIEHPGVAEACVVGAPDPEWGEIVVAFIVG-
MSMEG_5633|M.smegmatis_MC2_155 KDVVISGGSNIYPREVEEVLLEHPDVVEAGVVGAPDAEWGEIVVAFVVTN
MLBr_01051|M.leprae_Br4923 VDLIKLGGYRIGAGEIEMALLGHPDVREVAVVGLPDEDLGQR-IVAFVVG
MAV_3523|M.avium_104 KDMIITGGENVYGPEVESVLIEHPAVVDAAVIGVPDDFWGES-VKAIVVA
Mb0172|M.bovis_AF2122/97 KDMIISGGENIYCAELENVLASHPDIAEVAVIGRADEKWGEVPIAVAAVT
Rv0166|M.tuberculosis_H37Rv KDMIISGGENIYCAELENVLASHPDIAEVAVIGRADEKWGEVPIAVAAVT
MMAR_0409|M.marinum_M KDMIISGGENVYCAEVENVLASHPSIVEVAVIGRADEKWGEVPIAVAAVT
MAB_4569c|M.abscessus_ATCC_199 KDMIISGGENIYCAEVENALAEHEQIAEVAVIGRPHEKWGEVPVAVAAIH
MUL_0650|M.ulcerans_Agy99 KDMIVTGGFNVFPREVEDVVAEHSAVAQVCVVGAPDEKWGEAVTAVVVLR
*:: ** .: *:* .: * : :. *:* .. *: .
Mflv_1782|M.gilvum_PYR-GCK --DVGVAELDAHLLERIARFKR--------PKRYEFVDELPKNSYGKVLK
Mvan_4968|M.vanbaalenii_PYR-1 --AASAADLDAHLLERIARFKR--------PKRYEFVDELPKNSYGKVLK
TH_3645|M.thermoresistible__bu --SATAEELDAHLLDRIARFKR--------PKRYEFIDELPKSSYGKVLK
MSMEG_5633|M.smegmatis_MC2_155 GTPVDAAALDAHLLERIARFKR--------PKRYEFIDALPKNSYGKVLK
MLBr_01051|M.leprae_Br4923 AEALDADELINYVAQQLSIHKR--------PREVRFVDALPRNAMGKVLK
MAV_3523|M.avium_104 DGDVDAADVIEFCRRHLAGFKC--------PRTVDFVAELPRNASGKILK
Mb0172|M.bovis_AF2122/97 NDDLRIEDLGEFLTDRLARYKH--------PKALEIVDALPRNPAGKVLK
Rv0166|M.tuberculosis_H37Rv NDDLRIEDLGEFLTDRLARYKH--------PKALEIVDALPRNPAGKVLK
MMAR_0409|M.marinum_M KDHLRIEDLDEYLTERLARYKH--------PKALEIVDALPRNPSGKVLK
MAB_4569c|M.abscessus_ATCC_199 GAALTLAELDGFLTARLARYKH--------PKDLVIVDALPRNPSGKVLK
MUL_0650|M.ulcerans_Agy99 ADAARDDAAIETMTAEIQAAVKQRKGSVQSPKQVVVVDSLPLTGLGKPDK
.: *: .: ** . ** *
Mflv_1782|M.gilvum_PYR-GCK RELRERLK------------------------
Mvan_4968|M.vanbaalenii_PYR-1 RELRARLG------------------------
TH_3645|M.thermoresistible__bu RELRARLS------------------------
MSMEG_5633|M.smegmatis_MC2_155 RELRERVTG-----------------------
MLBr_01051|M.leprae_Br4923 KQLLFEG-------------------------
MAV_3523|M.avium_104 TQLREPFWRDRDRRV-----------------
Mb0172|M.bovis_AF2122/97 TELRLRYGACVNVERRSASAGFTERRENRQKL
Rv0166|M.tuberculosis_H37Rv TELRLRYGACVNVERRSASAGFTERRENRQKL
MMAR_0409|M.marinum_M TELRLRYGVGVYSESRSVSRSFEFREGS----
MAB_4569c|M.abscessus_ATCC_199 TELRQRFGIAD---------------------
MUL_0650|M.ulcerans_Agy99 KAVRARFWEGAGRAVG----------------
: