For questions or suggestions e-mail us at: ioerger@cs.tamu.edu
TQTTASPQPRTHLAAVLADAVIDDPEAGVFRANRRIFTDEEIFELEMKHIFEGNWIYLAHESQVANPGDY FTTYIGRQPVVITRSKDGSLNCLINACAHRGAMICRRKTDNRMTLTCPFHGWTFRNDGALLKVKDPDGAG YPEAFNVNGSHNMTTVARFESYRGFLFGSLNPDVLPLTEHLGDATKIIDMLVDQSPDGLEVLRGSSTYTY DGNWKVQAENGADGYHVTATHWNYAATTSRRGTGESKNETKALDAGGWGKSGGGYWSFPHGHLCLWTWAA NPQDRPLWDKLDELKATHGDAKGEFMVKGSRNLCVYPNVYVMDQFSTQIRHFRPIAPDKTEVTIYCIAPK SESAQARANRIRQYEDFFNASGMATPDDLEEFRSCQLTFRAEAAPWNDMSRGAQHWLDGPDDVARSLGLD AVISAGVKNEDEGLYPVQHGYWLETMRAAAQKEAR*
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. gilvum PYR-GCK | Mflv_1355 | - | - | 100% (456) | Rieske (2Fe-2S) domain-containing protein |
| M. gilvum PYR-GCK | Mflv_0595 | - | 1e-41 | 28.61% (374) | ring hydroxylating dioxygenase, alpha subunit |
| M. gilvum PYR-GCK | Mflv_0685 | - | 7e-41 | 28.02% (389) | ring hydroxylating dioxygenase, alpha subunit |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb3186c | - | 1e-21 | 30.85% (201) | dioxygenase |
| M. tuberculosis H37Rv | Rv3161c | - | 4e-22 | 31.34% (201) | dioxygenase |
| M. leprae Br4923 | - | - | - | - | - |
| M. abscessus ATCC 19977 | MAB_4370c | - | 3e-45 | 29.87% (395) | putative large terminal subunit of phenylpropionate dioxygenase |
| M. marinum M | MMAR_4422 | - | 3e-20 | 27.96% (211) | dioxygenase |
| M. avium 104 | MAV_2612 | - | 1e-51 | 32.03% (434) | dioxygenase large subunit |
| M. smegmatis MC2 155 | MSMEG_1908 | - | 0.0 | 89.04% (438) | benzoate 1,2-dioxygenase alpha subunit |
| M. thermoresistible (build 8) | TH_2976 | - | 9e-44 | 30.34% (379) | PUTATIVE dioxygenase large subunit |
| M. ulcerans Agy99 | MUL_3820 | - | 1e-13 | 29.57% (186) | ring-hydroxylating dioxygenase, large terminal subunit |
| M. vanbaalenii PYR-1 | Mvan_4415 | - | 2e-41 | 28.79% (389) | ring hydroxylating dioxygenase, alpha subunit |
CLUSTAL 2.0.9 multiple sequence alignment
Mb3186c|M.bovis_AF2122/97 --------MLSTDNRAELGDILTDIGDYLDDNPPALSLPPAAYTSSELWQ
Rv3161c|M.tuberculosis_H37Rv --------MLSTDNRAELGDILTDIGDYLDDNPPALSLPPAAYTSSELWQ
Mflv_1355|M.gilvum_PYR-GCK ---MTQTTASPQPRTHLAAVLADAVIDDPEAGVFR--ANRRIFTDEEIFE
MSMEG_1908|M.smegmatis_MC2_155 ----MTTHLSP---DHLENVLADAVIEDPAAGVYR--ANRRIFTDDEIFE
MAV_2612|M.avium_104 ------------MTTLESAPAVDDFDVGSVIRHDR--VHGTVYTSTEIFR
TH_2976|M.thermoresistible__bu -----------------------------LIRPDR--VHGSLYTDPAIFA
MAB_4370c|M.abscessus_ATCC_199 MASLPRPVAVAQGALCRPQALVRDIDVSGLVDSHGGLLDRVIFTDGELYR
Mvan_4415|M.vanbaalenii_PYR-1 -----------------------MIDFTDLVDPEHGWVSPQIYTDPEIYE
MMAR_4422|M.marinum_M ------------------------MTAVRQPSALLPTLGGAYYTSAAVFA
MUL_3820|M.ulcerans_Agy99 -------MDRDQLIDLTRRALKLARDKTTDLAPHQHAVDAREYTSLDRHE
:*.
Mb3186c|M.bovis_AF2122/97 LERERIFNRSWMLVAHVDQLAKTGDYVTVSVAGEPVMVVRDVDGQLHALS
Rv3161c|M.tuberculosis_H37Rv LERERIFNRSWMLVAHVDQVAKTGDYVTVSVAGEPVMVVRDVDGQLHALS
Mflv_1355|M.gilvum_PYR-GCK LEMKHIFEGNWIYLAHESQVANPGDYFTTYIGRQPVVITRSKDGSLNCLI
MSMEG_1908|M.smegmatis_MC2_155 LEMKHIFEGNWVYLAHESQVPNPGDYFTTYIGRQPVVITRDRDGDLHCLI
MAV_2612|M.avium_104 REMDTIFKTGWVYVAHESEVSEPGDYLTRMIGHDPVVVARGKDGVVRVML
TH_2976|M.thermoresistible__bu EELRKIWYRTWVFVGHESEVSQPNDYVRKKLGLQDVIMTRDRDGEIHLLL
MAB_4370c|M.abscessus_ATCC_199 QELRRVFAPSWLFLAHADQFHRPGDFFTTYMGEDPVIVSMDKNRRIRAFL
Mvan_4415|M.vanbaalenii_PYR-1 RELQHVFGRSWLFLAHDSQLPKPGSFLQTYMGEDPVLVVRQRDGSVRAFL
MMAR_4422|M.marinum_M AEQQHVFESMWFCVVRAADLNEPGQFKAVQVGRESVLLVRGRDRRLRAFL
MUL_3820|M.ulcerans_Agy99 RDRAMVLD-SPQLVGYSSELPGPDTYRTKTIMGRSILLTRAADRAVRAFD
: : : :. .. : : ::: : :. :
Mb3186c|M.bovis_AF2122/97 PICRHRLMLMVEPGAGRID-TLTCQYHLWRYGLDGRLRGAPHMAANLDFN
Rv3161c|M.tuberculosis_H37Rv PICRHRLMLMVEPGAGRID-TLTCQYHLWRYGLDGRLRGAPHMAANLDFN
Mflv_1355|M.gilvum_PYR-GCK NACAHRGAMICRRKTDNRM-TLTCPFHGWTFRNDGALLKVKDPDGAGYPE
MSMEG_1908|M.smegmatis_MC2_155 NACAHRGAMICRRKTDNRT-TLTCPFHGWTFRNDGTLLKVKDPDGAGYPD
MAV_2612|M.avium_104 NRCTHRANKLCNAEKGNAN-SFRCPYHGWTFGNTGALQGVPMREGYGEEF
TH_2976|M.thermoresistible__bu NRCSHRGNQVCADAKGNSS-TFRCPYHGWTFRNTGELVGFPFFKGYGTR-
MAB_4370c|M.abscessus_ATCC_199 NSCRHRGARVCRADYGVTR-GFTCTYHGWAYDLEGKLISVPNEE-GYPDS
Mvan_4415|M.vanbaalenii_PYR-1 NQCRHRGMRICRSEAGVSK-AFTCTYHGWSYDLAGNLINVPLEERAYHSS
MMAR_4422|M.marinum_M NICRHRGALLCTEPEGQVRRNLRCPYHSWTYGLDGTLIAAPNIAELTDTD
MUL_3820|M.ulcerans_Agy99 NVCLHRQSRVATGCGTALR--FTCPYHGWTYDNGGRLVGLPGREGFPGIA
* ** : : * :* * : * *
Mb3186c|M.bovis_AF2122/97 ----RRECRLPQFA-VATWNGLVWINLDADA-EPIAAHLD-LTDDEFAGY
Rv3161c|M.tuberculosis_H37Rv ----RRECRLPQFA-VATWNGLVWINLDADA-EPIAAHLD-LTDDEFAGY
Mflv_1355|M.gilvum_PYR-GCK AFNVNGSHNMTTVARFESYRGFLFGSLNPDV-LPLTEHLG-DATKIIDML
MSMEG_1908|M.smegmatis_MC2_155 TFNSNGSHNMTNVARFEGYRGFLFGSLNPDV-LPLTEHLG-DTTTVIDML
MAV_2612|M.avium_104 NK-VRSELGLAQAPRVDSYGGFVFASLAPDG-ISLTEHLG-KATRAIDRL
TH_2976|M.thermoresistible__bu ----KLDLPLGRVPRVDSYGGFVFGSFATEG-PTLREHLG-AAAGEIDRL
MAB_4370c|M.abscessus_ATCC_199 FS--TDDWGLVEVPHVDSYHGLIFGTWNPAP-VGLRESLG-DMAWYMDAM
Mvan_4415|M.vanbaalenii_PYR-1 ID--KKEWGAMKVPRVANYRGFYFGTWSEET-PEFDAYLG-DMAFYFDAI
MMAR_4422|M.marinum_M GASIDRHRYGLVAVALREWLGYAWVCLAEDP-PSFEDDVVGSVTARLGDV
MUL_3820|M.ulcerans_Agy99 VG-----SDGLTELPATEFAGFLWVSLHPAARLDVPAHLG-PLAGELQSW
: * : . : :
Mb3186c|M.bovis_AF2122/97 RLG-----EMVQVES-WSHEWRANWKVAAEN-GHENYHVLGLHRQTLEPF
Rv3161c|M.tuberculosis_H37Rv RLG-----EMVQVES-WSHEWRANWKVAAEN-GHENYHVLGLHRQTLEPF
Mflv_1355|M.gilvum_PYR-GCK VDQSPDG-LEVLRGS-STYTYDGNWKVQAEN-GADGYHVTATHWN-YAAT
MSMEG_1908|M.smegmatis_MC2_155 VDQSPDG-LEVLRGA-STYTYDGNWKVQAEN-GADGYHVTATHWN-YAAT
MAV_2612|M.avium_104 LNLSPTREIDLRANW-MKHLHHANWKMVVEN-NVDGYHALFTHASVYDAI
TH_2976|M.thermoresistible__bu LRLSPEGKVALDAGW-LRHRTRANWKLLVEN-ETDGYHPQFVHGGIFGVT
MAB_4370c|M.abscessus_ATCC_199 LDHDSEG-TVVVGGV-HKWVLDGNWKLAAEQFATDWYHVNMSHASALMVM
Mvan_4415|M.vanbaalenii_PYR-1 VDRFDSG-LEFVKGT-TKWVIDCNWKFASEQFASDMYHTQSAHASALLAL
MMAR_4422|M.marinum_M SAIDTYRIEALQVGRRVSYDVAANWKLIVEN-FMECYHCATIHPELTRVI
MUL_3820|M.ulcerans_Agy99 GIGRWSPLGEKVLGS------PIDWKLATDT-FAENYHFATVHRRTFATI
:**. : : ** *
Mb3186c|M.bovis_AF2122/97 VPGGGDLD-----------------------------VRQYSRWALRLRV
Rv3161c|M.tuberculosis_H37Rv VPGGGDLD-----------------------------VRQYSRWALRLRV
Mflv_1355|M.gilvum_PYR-GCK TSRRGTGESKNE--TKALDAGGWGKSGGGYWSFPHGHLCLWTWAANPQDR
MSMEG_1908|M.smegmatis_MC2_155 TSRRTCGESKND--TKALDAGSWGKSGGGYWSFPHGHLCLWTWAANPQDR
MAV_2612|M.avium_104 KPAKVSHVPTKV--DVLVRDLGNGHSEIDYSDEYRKLDEEFVWYGRIPRA
TH_2976|M.thermoresistible__bu GSTIGPLYSDSS--TAVTRDLGGGHSENDLRPEFRKFAEPLRWFG-TTES
MAB_4370c|M.abscessus_ATCC_199 A--PQGKAPKDEIVHRTGRQYVDPNGHGAG-----FPVHPKNRFDARVVH
Mvan_4415|M.vanbaalenii_PYR-1 TDDPNPIGPLND-PNVPGRQFSG-NGHGSGGYFLPAPVVKTPEMTDTMFE
MMAR_4422|M.marinum_M PEFARGQAAQRS-------------------------VGRGAEFGSAVAG
MUL_3820|M.ulcerans_Agy99 ARSNCTVFDSYG----------------------------------PHHR
Mb3186c|M.bovis_AF2122/97 PFTVPVEAKSLQLNEVQKSNLVVLWT-----FPNSALAIAG--ERVVWFG
Rv3161c|M.tuberculosis_H37Rv PFTVPVEAKSLQLNEVQKSNLVVLWT-----FPNSALAIAG--ERVVWFG
Mflv_1355|M.gilvum_PYR-GCK PLWDKLDELKATHG-DAKGEFMVKGSRNLCVYPNVYVMDQFSTQIRHFRP
MSMEG_1908|M.smegmatis_MC2_155 PLWDRLDELKARFG-DAKGEFMVKGSRNLCLYPNVYVMDQFSTQIRHFRP
MAV_2612|M.avium_104 KLADYVGALEGAYGPEQAHDALVVGPPHTLIWPNLFLAEMN---VMFVEP
TH_2976|M.thermoresistible__bu RVPDYVAAMRARYG-AEADQILIEGSPHVMIFPNLFIAEIQ---VFNIQP
MAB_4370c|M.abscessus_ATCC_199 QWTDYG-ALRERLGEARVQGPLTNG--HATVFPNLSYLPVNG-SVRIWHP
Mvan_4415|M.vanbaalenii_PYR-1 WFKSREEEMVARIGADRLS-KVSIT--HNTIFPNFSWLGAHS-TMRVWHP
MMAR_4422|M.marinum_M FTVDGRAGFTALPGIRPEQDRRYFA---ITVKPTVFINLVP--DHAIIHR
MUL_3820|M.ulcerans_Agy99 LIFPLNTILELENVPEGQWDPFHHMVVIYALFPNVVLSVTIA--NGELFR
*.
Mb3186c|M.bovis_AF2122/97 FIPQSIDRVQVLGGVLTTPELAADAAATAQTSQFVMA-MINDEDRLGLEA
Rv3161c|M.tuberculosis_H37Rv FIPQSIDRVQVLGGVLTTPELAADAAATAQTSQFVMA-MINDEDRLGLEA
Mflv_1355|M.gilvum_PYR-GCK IAPDKTEVTIYCIAPKSESAQARANRIRQYEDFFNASGMATPDDLEEFRS
MSMEG_1908|M.smegmatis_MC2_155 IAPDKTEVTIYCIAPKGESAAARAHRIRQYEDFFNASGMATPDDLEEFRS
MAV_2612|M.avium_104 QAPDRTIAYTTAVLIPGQDALN-ERTLRRSEGAMGPAGFLIADDGEIGMR
TH_2976|M.thermoresistible__bu VAVNECVQYSTAVQLVGAPELN-RRMVSQCIGSVGPAGMLLADDTEMYER
MAB_4370c|M.abscessus_ATCC_199 KGPDKMEVWAWTIVDRSMPEEVRDAQRLYNLRTFGPTGIFEQDDGENWSE
Mvan_4415|M.vanbaalenii_PYR-1 RGPGQIEVWAWTYVPKDAPPKVKNEIRELTQRTFSPAGSFETDDGENWTE
MMAR_4422|M.marinum_M MFPIAADRTIVECDWLYAPEVAAAGDIAHSVELFHRV---NEQDFDACQR
MUL_3820|M.ulcerans_Agy99 IYPGDRPGSSITVHQNSTPLDLSDESVATGAQAVFDYAHATVRDEDYRLA
. *
Mb3186c|M.bovis_AF2122/97 VQVGAGSRFAERGHLSSK-------------------------------E
Rv3161c|M.tuberculosis_H37Rv VQVGAGSRFAERGHLSSK-------------------------------E
Mflv_1355|M.gilvum_PYR-GCK CQLTFRAEAAPWNDMSRGAQHWLDGPDDVARSLGLDAVISAGVKNEDEGL
MSMEG_1908|M.smegmatis_MC2_155 CQLTFRAQAAPWNDMSRGAQHWLSGPDPVAESLGMHGIVSSGLRNEDEGL
MAV_2612|M.avium_104 NQAGLAAELPEWLMLARG--------------IESDVDDMTGIINRDKSA
TH_2976|M.thermoresistible__bu NQQGLEALTPEWLDIRRG--------------LNREHVDDHGFTVGGPTD
MAB_4370c|M.abscessus_ATCC_199 VQSISRGFVTNSVALNYQMG---------LDSEREDGR-HPGLTS-ELYS
Mvan_4415|M.vanbaalenii_PYR-1 IQQVLRGSQARRNRLHTAMG---------VGYEERDAFGLPGLGN-DVYS
MMAR_4422|M.marinum_M TQPAMSSRAYRTGGVLVP-------------------------------A
MUL_3820|M.ulcerans_Agy99 ETLQTNLESGARQRLVFG------------------------------RN
:
Mb3186c|M.bovis_AF2122/97 WPGMLAFYRNLAMALVGDHPGAS-------------------
Rv3161c|M.tuberculosis_H37Rv WPGMLAFYRNLAMALVGDHPGAS-------------------
Mflv_1355|M.gilvum_PYR-GCK YPVQHGYWLETMRAAAQKEAR---------------------
MSMEG_1908|M.smegmatis_MC2_155 YPVQHGYWLETLRRAVTSNPTEH-------------------
MAV_2612|M.avium_104 ETPQRGFYSHWASVVGGRSGA---------------------
TH_2976|M.thermoresistible__bu ETGMRAFWSEYRRLMEAE------------------------
MAB_4370c|M.abscessus_ATCC_199 DAAGRSFYARWRDLMNTKAWHEEHV-----------------
Mvan_4415|M.vanbaalenii_PYR-1 ETAARGFYRHWLDMLTGKPWSEIQKWTPNGNHGELRDEGVTA
MMAR_4422|M.marinum_M EHHIAEFHQWVIARIGTPAVTG--------------------
MUL_3820|M.ulcerans_Agy99 EPGLQHRHITWAQALETPG-----------------------