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QDHGEVLAAVRTGMIPAHVYNDKQIFSLEKERLFSRAWLFVAHESEIPQPGDYVVRQVLQDSFIVARDSA GEVRVMFNMCLHRGMQVCRAEMGNASNFRCPYHGWSYRNDGRIIGLPFHQEAYGGDAGFNKAGQTLLPAP SVASYNGLIFLSMDPDAESLEDYLGDFRFYLDFYTKQGPNGLEVRGPQRWRVKANWKIAAENFAGDMYHT PQTHTSVVEIGLFREPKANKRKDGATYWAGRGGGTTYKLPEGSFEDRMSYVGYPADMISRAKATWTEQQQ QVVGTDGFMISAATCFPNISFVHNWPKVEDGEHVLPFISIRVWQPISENETEVLSWFAVDSDAPADFKAD SYKAYLMCFGSTGMFEQDDVENWVSLTNTAGGSMARRLRLNSRMGLLADDVRVVDTLSSAQFHGPGYAQL GYNENNQRQLLRLWADYLDMPPLRVDPATVLSDNPHGIEPMVQTNGAAAADIDSGSAESVML*
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. gilvum PYR-GCK | Mflv_0595 | - | - | 100% (483) | ring hydroxylating dioxygenase, alpha subunit |
| M. gilvum PYR-GCK | Mflv_0685 | - | 0.0 | 97.07% (478) | ring hydroxylating dioxygenase, alpha subunit |
| M. gilvum PYR-GCK | Mflv_0662 | - | 1e-90 | 40.72% (442) | ring hydroxylating dioxygenase, alpha subunit |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb3186c | - | 3e-26 | 33.01% (209) | dioxygenase |
| M. tuberculosis H37Rv | Rv3161c | - | 2e-26 | 33.01% (209) | dioxygenase |
| M. leprae Br4923 | - | - | - | - | - |
| M. abscessus ATCC 19977 | MAB_4370c | - | 2e-53 | 31.21% (439) | putative large terminal subunit of phenylpropionate dioxygenase |
| M. marinum M | MMAR_0307 | - | 4e-23 | 28.70% (230) | dioxygenase |
| M. avium 104 | MAV_3509 | - | 0.0 | 75.40% (435) | phthalate dioxygenase large subunit |
| M. smegmatis MC2 155 | MSMEG_1908 | - | 4e-42 | 28.15% (373) | benzoate 1,2-dioxygenase alpha subunit |
| M. thermoresistible (build 8) | TH_2976 | - | 4e-37 | 29.57% (372) | PUTATIVE dioxygenase large subunit |
| M. ulcerans Agy99 | MUL_3820 | - | 1e-17 | 33.90% (177) | ring-hydroxylating dioxygenase, large terminal subunit |
| M. vanbaalenii PYR-1 | Mvan_0463 | - | 0.0 | 96.23% (478) | ring hydroxylating dioxygenase, alpha subunit |
CLUSTAL 2.0.9 multiple sequence alignment
Mflv_0595|M.gilvum_PYR-GCK ----------MQDHG----EVLAAVRTGM--------IPAHVYNDKQIFS
Mvan_0463|M.vanbaalenii_PYR-1 ----------MQDHG----EVLAAVRTGM--------IPAHVYNDKQIFS
MAV_3509|M.avium_104 ----------MAGPDGGLLGVLENVRRGM--------IPAHIYNDPELFA
Mb3186c|M.bovis_AF2122/97 --------MLSTDNRAELGDILTDIGDYLDDNPPALSLPPAAYTSSELWQ
Rv3161c|M.tuberculosis_H37Rv --------MLSTDNRAELGDILTDIGDYLDDNPPALSLPPAAYTSSELWQ
MAB_4370c|M.abscessus_ATCC_199 MASLPRPVAVAQGALCRPQALVRDIDVSGLVDSHGGLLDRVIFTDGELYR
TH_2976|M.thermoresistible__bu --------------------LIRPDRVHG-----------SLYTDPAIFA
MSMEG_1908|M.smegmatis_MC2_155 ---MTTHLSPDHLENVLADAVIEDPAAGVYR------ANRRIFTDDEIFE
MUL_3820|M.ulcerans_Agy99 -------MDRDQLIDLTRRALKLARDKTTDLAPHQHAVDAREYTSLDRHE
MMAR_0307|M.marinum_M --------MTDHNDGRAEPMDAAHHDDVEATLTGAVTFGVDAYTCADYAR
:.
Mflv_0595|M.gilvum_PYR-GCK LEKERLFSRAWLFVAHESEIPQPGDYVVRQVLQDSFIVARDSAGEVRVMF
Mvan_0463|M.vanbaalenii_PYR-1 LEKERLFSRAWLFVAHESEIPQPGDYVVRQVLQDSFIIARDSAGEVRVMF
MAV_3509|M.avium_104 LEKRRLFARAWTFVGHESEIPHDGDYMVRRVLDDSFIITRGSDGRVRALF
Mb3186c|M.bovis_AF2122/97 LERERIFNRSWMLVAHVDQLAKTGDYVTVSVAGEPVMVVRDVDGQLHALS
Rv3161c|M.tuberculosis_H37Rv LERERIFNRSWMLVAHVDQVAKTGDYVTVSVAGEPVMVVRDVDGQLHALS
MAB_4370c|M.abscessus_ATCC_199 QELRRVFAPSWLFLAHADQFHRPGDFFTTYMGEDPVIVSMDKNRRIRAFL
TH_2976|M.thermoresistible__bu EELRKIWYRTWVFVGHESEVSQPNDYVRKKLGLQDVIMTRDRDGEIHLLL
MSMEG_1908|M.smegmatis_MC2_155 LEMKHIFEGNWVYLAHESQVPNPGDYFTTYIGRQPVVITRDRDGDLHCLI
MUL_3820|M.ulcerans_Agy99 RDRAMVLD-SPQLVGYSSELPGPDTYRTKTIMGRSILLTRAADRAVRAFD
MMAR_0307|M.marinum_M AERDKLWRKVWQQVGRVEDLPKVGSYLTYDILDDSIVVVRSAADTFKAHH
: : :. .:. . : : .:: .:
Mflv_0595|M.gilvum_PYR-GCK NMCLHRGM------QVCRAEMGNASNFRCPYHGWSYRNDGRIIGLPFHQE
Mvan_0463|M.vanbaalenii_PYR-1 NMCLHRGM------QVCRAEMGNASNFRCPYHGWSYRNDGRIIGLPFHQE
MAV_3509|M.avium_104 NMCLHRGM------QVCRAELGNASNFRCPYHGWTYRNDGRLTGLPFHRE
Mb3186c|M.bovis_AF2122/97 PICRHRLM------LMVEPGAGRIDTLTCQYHLWRYGLDGRLRGAPHMAA
Rv3161c|M.tuberculosis_H37Rv PICRHRLM------LMVEPGAGRIDTLTCQYHLWRYGLDGRLRGAPHMAA
MAB_4370c|M.abscessus_ATCC_199 NSCRHRGA------RVCRADYGVTRGFTCTYHGWAYDLEGKLISVPNEEG
TH_2976|M.thermoresistible__bu NRCSHRGN------QVCADAKGNSSTFRCPYHGWTFRNTGELVGFPFFKG
MSMEG_1908|M.smegmatis_MC2_155 NACAHRGA------MICRRKTDNRTTLTCPFHGWTFRNDGTLLKVKDPDG
MUL_3820|M.ulcerans_Agy99 NVCLHRQS------RVATG-CGTALRFTCPYHGWTYDNGGRLVGLPGREG
MMAR_0307|M.marinum_M NVCMHRGRRLVDIPPGAKNVCGHKKSFVCGFHGWTYDQDGNCIHIPEKPD
* ** . : * :* * : *
Mflv_0595|M.gilvum_PYR-GCK AYGGDAGFNKAGQTLLPAPSVASYNGLIFLSMDPDAESLEDYLGDFRFYL
Mvan_0463|M.vanbaalenii_PYR-1 AYGGDAGFNKTGQTLLPAPSVASYNGLIFLSMDPDAESLEDYLGDFRFYL
MAV_3509|M.avium_104 AYGGDDGFVKD-QTLLPAPNFAGYNGLLFISLDPAAPPLQDFLGDFRFYL
Mb3186c|M.bovis_AF2122/97 NLD----FNRR-ECRLPQFAVATWNGLVWINLDADAEPIAAHL---DLTD
Rv3161c|M.tuberculosis_H37Rv NLD----FNRR-ECRLPQFAVATWNGLVWINLDADAEPIAAHL---DLTD
MAB_4370c|M.abscessus_ATCC_199 YPDS---FSTDDWGLVEVPHVDSYHGLIFGTWNPAPVGLRESLGDMAWYM
TH_2976|M.thermoresistible__bu YGTR-----KLDLPLGRVPRVDSYGGFVFGSFATEGPTLREHLGAAAGEI
MSMEG_1908|M.smegmatis_MC2_155 AGYPDTFNSNGSHNMTNVARFEGYRGFLFGSLNPDVLPLTEHLGDTTTVI
MUL_3820|M.ulcerans_Agy99 FPG-----IAVGSDGLTELPATEFAGFLWVSLHPAAR--LDVPAHLGPLA
MMAR_0307|M.marinum_M WQG----ALTPENTHLAAVNVDTWGGWIWINMDPAAQPLRQYLEPAATML
: * :: . .
Mflv_0595|M.gilvum_PYR-GCK DFYTKQGPNGLEVRG--PQRWRVKANWKIAAENFAGDMYHTPQTHTSVVE
Mvan_0463|M.vanbaalenii_PYR-1 DFYTRQGPNGLEVQG--PQRWRVKANWKIAAENFAGDMYHTPQTHTSVVE
MAV_3509|M.avium_104 DYYTRQSAGGVELRG--PQRWRIRANWKIGAENFAGDMYHTPHTHASIVD
Mb3186c|M.bovis_AF2122/97 DEFAGYRLGEMVQVE--SWSHEWRANWKVAAEN-GHENYHVLGLHRQTLE
Rv3161c|M.tuberculosis_H37Rv DEFAGYRLGEMVQVE--SWSHEWRANWKVAAEN-GHENYHVLGLHRQTLE
MAB_4370c|M.abscessus_ATCC_199 DAMLDHDSEGTVVVGG-VHKWVLDGNWKLAAEQFATDWYHVNMSHASALM
TH_2976|M.thermoresistible__bu DRLLRLSPEGKVALDAGWLRHRTRANWKLLVEN-ETDGYHPQFVHGGIFG
MSMEG_1908|M.smegmatis_MC2_155 DMLVDQSPDGLEVLRG-ASTYTYDGNWKVQAEN-GADGYHVTATHWNYAA
MUL_3820|M.ulcerans_Agy99 GELQSWGIGRWSPLG--EKVLGSPIDWKLATDT-FAENYHFATVHRRTFA
MMAR_0307|M.marinum_M DPFRLQDMR-----CKWRKWLTFQCNWKVAMEA-FNETYHVATTHPQFNK
. :**: : : ** *
Mflv_0595|M.gilvum_PYR-GCK IGLFREPKANKRKDGATYWAGRGGGTTYKLPEGSFEDRMSY-VGYPADMI
Mvan_0463|M.vanbaalenii_PYR-1 IGLFREPKAHKRKDGATYWAGRGGGTTYKLPEGSFEDRMSY-VGYPAEMI
MAV_3509|M.avium_104 IGLFREPKAQKRKDGATYWAHRGGGTTYKLPPGGFEERMRY-VGYPDEMI
Mb3186c|M.bovis_AF2122/97 P---------------------------FVPGGGDLDVRQY-SRWALRLR
Rv3161c|M.tuberculosis_H37Rv P---------------------------FVPGGGDLDVRQY-SRWALRLR
MAB_4370c|M.abscessus_ATCC_199 VMAPQ-----------------GKAPKDEIVHRTGRQYVDP-NGHGAGFP
TH_2976|M.thermoresistible__bu VTGSTIG-------------PLYSDSSTAVTRDLGGGHSEN-DLRPEFRK
MSMEG_1908|M.smegmatis_MC2_155 TTSRRTCG------------ESKNDTKALDAGSWGKSGGGY-WSFPHGHL
MUL_3820|M.ulcerans_Agy99 T----------------------------IARSNCTVFDSYGPHHRLIFP
MMAR_0307|M.marinum_M FGNFRG-------------WAAAHGRHSNIGYDAPKDLAQTKSKIRLGTG
Mflv_0595|M.gilvum_PYR-GCK SRAKATWTEQQQQVVGTDGFMISAATCFPNISFVHNWPKVEDG--EHVLP
Mvan_0463|M.vanbaalenii_PYR-1 SRAKATWTEQQRQVVGADGFMISAATCFPNISFVHNWPKVEDG--EHVLP
MAV_3509|M.avium_104 GRIKQVWTPRQQRVVGEDGFMVSAATCFPNLSFVHNWPKVRDGRDDETLP
Mb3186c|M.bovis_AF2122/97 VPFTVPVEAKSLQLNEVQKSNLVVLWTFPNSALAIAG--------ERVVW
Rv3161c|M.tuberculosis_H37Rv VPFTVPVEAKSLQLNEVQKSNLVVLWTFPNSALAIAG--------ERVVW
MAB_4370c|M.abscessus_ATCC_199 VHPKNRFDARVVHQWTDYGALRERLGEARVQGPLTNGHATVFPNLSYLPV
TH_2976|M.thermoresistible__bu FAEPLRWFGTTESRVPDYVAAMRARYGAEADQILIEGSPHVMIFPNLFIA
MSMEG_1908|M.smegmatis_MC2_155 CLWTWAANPQDRPLWDRLDELKARFGDAKGEFMVKGSRNLCLYPNVYVMD
MUL_3820|M.ulcerans_Agy99 LNTILELENVPEGQWDPFHHMVVIYALFPNVVLSVTIAN------GELFR
MMAR_0307|M.marinum_M ADPRVSTAEMQRYTLEETNATTTRTLVDAALRLVEELPEDAPAGQVLDHW
Mflv_0595|M.gilvum_PYR-GCK FIS-------IRVWQPISENETEVLSWFAVDSDAPADFKADSYKAYLMCF
Mvan_0463|M.vanbaalenii_PYR-1 FIS-------IRVWQPISENETEVLSWFAVDSDAPEAFKAASYKAYLMCF
MAV_3509|M.avium_104 FIS-------IRLWQPISENETEVCSWFAVDSAAPAHYKQDSYKAYLMCF
Mb3186c|M.bovis_AF2122/97 FG-----------FIPQSIDRVQVLGGVLTTPELAADAAATAQTSQFVMA
Rv3161c|M.tuberculosis_H37Rv FG-----------FIPQSIDRVQVLGGVLTTPELAADAAATAQTSQFVMA
MAB_4370c|M.abscessus_ATCC_199 NGS-------VRIWHPKGPDKMEVWAWTIVDRSMPEEVRDAQRLYNLRTF
TH_2976|M.thermoresistible__bu EIQ-------VFNIQPVAVNECVQYS-TAVQLVGAPELNRRMVSQCIGSV
MSMEG_1908|M.smegmatis_MC2_155 QFSTQ-----IRHFRPIAPDKTEVTIYCIAPKGESAAARAHRIRQYEDFF
MUL_3820|M.ulcerans_Agy99 IYP------------GDRPGSSITVHQNSTPLDLSDESVATGAQAVFDYA
MMAR_0307|M.marinum_M LTSARRDDAARGVLWPTIDPEHLAQSGTAWQIFPNFQIGQGLTTALCYSA
Mflv_0595|M.gilvum_PYR-GCK GSTGMFEQDDVENWVSLTNTAGGSMARRLRLNSRMGLLADDVRVVDTLSS
Mvan_0463|M.vanbaalenii_PYR-1 GSTGMFEQDDVENWVSLTNTAGGSMARRLRLNSRMGLLADDARVVDTLSS
MAV_3509|M.avium_104 GSTGMFEQDDVENWVSLTTTAGGSMARRLLLNSRMGLYDDGRPVVEALAP
Mb3186c|M.bovis_AF2122/97 ----MINDEDRLGLEAVQVGAGSRFAERGHLSSKE---------------
Rv3161c|M.tuberculosis_H37Rv ----MINDEDRLGLEAVQVGAGSRFAERGHLSSKE---------------
MAB_4370c|M.abscessus_ATCC_199 GPTGIFEQDDGENWSEVQSISRGFVTNSVALNYQMGLDSE---------R
TH_2976|M.thermoresistible__bu GPAGMLLADDTEMYERNQQGLEALTPEWLDIRRGLN-------------R
MSMEG_1908|M.smegmatis_MC2_155 NASGMATPDDLEEFRSCQLTFRAQAAPWNDMSRGAQHWLSG----PDPVA
MUL_3820|M.ulcerans_Agy99 HAT--VRDEDYRLAETLQTNLESGARQRLVFGRNE---------------
MMAR_0307|M.marinum_M RPHGYDPDQCVFEASCYELYPKGEEPHTEWVYAPPDDPGWRSVLPQDFSN
: . .
Mflv_0595|M.gilvum_PYR-GCK AQFHGPGYAQLGYNENNQRQLLRLWADYLDMPPLRVDPATVLSDNPHGIE
Mvan_0463|M.vanbaalenii_PYR-1 AQFHGPGYAQLGYNENNQRQLLRLWADYLDMPPLRVDPATVLTDNPQGIE
MAV_3509|M.avium_104 SAFHGPGRAQVGYNEHNQRALLAMWADYLQE-------------------
Mb3186c|M.bovis_AF2122/97 ----WPG--MLAFYRN---LAMALVGDHPGAS------------------
Rv3161c|M.tuberculosis_H37Rv ----WPG--MLAFYRN---LAMALVGDHPGAS------------------
MAB_4370c|M.abscessus_ATCC_199 EDGRHPGLTSELYSDAAGRSFYARWRDLMNTKAWHEEHV-----------
TH_2976|M.thermoresistible__bu EHVDDHGFTVGGPTDETG--MRAFWSEYRRLMEAE---------------
MSMEG_1908|M.smegmatis_MC2_155 ESLGMHGIVSSGLRNEDEGLYPVQHGYWLETLRRAVTSNPTEH-------
MUL_3820|M.ulcerans_Agy99 ----------PGLQHR-----HITWAQALETPG-----------------
MMAR_0307|M.marinum_M MAAVQQGMKSGGFGGPKPNPYMERSTANLHRNLAEYMGTGAPREL-----
Mflv_0595|M.gilvum_PYR-GCK PMVQTNGAAAADIDSGSAES-VML
Mvan_0463|M.vanbaalenii_PYR-1 PMVQTNGGAVAGIDSESATTSVTL
MAV_3509|M.avium_104 ------GDACENR-----------
Mb3186c|M.bovis_AF2122/97 ------------------------
Rv3161c|M.tuberculosis_H37Rv ------------------------
MAB_4370c|M.abscessus_ATCC_199 ------------------------
TH_2976|M.thermoresistible__bu ------------------------
MSMEG_1908|M.smegmatis_MC2_155 ------------------------
MUL_3820|M.ulcerans_Agy99 ------------------------
MMAR_0307|M.marinum_M ------------------------