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TAPTERRLANVLNGQYTPIDDDTAANWRAAGWWENRSIRSLLADAAQAHPDRIALVGRRADGGRVARTYQ EFDRNANQVASVLASLGVRPDDAVVVMLPNWVEYPEFLFGINELGAIYAGIPVAYGDQQAAAILRRSRAR VLVIPRRWRGNNILEQSRRLRDQIPTLQQVIVLDDDGTDLRDGESLWSDHAHVAARQFPPPDPGQICYLG FTSGTTGEPKGAMHSHNTLIYSARRQAEHIGTEAFGEPVVNLVASPMGHHTGYVWGGVFTVMLAGTAVHV DRWDPTWGAQVVREEGVTTFFGAPTFLQDIIRTELAGDPACPLRCMVVAGAPVPRNLPAQAAEALGAYVA PAWGMTECSILTSCTPDEPDAILRTDGSVFAGSEVKIVDDTGAAVAAGVVGDLLMRGPGVVYGYYDRPDA TRDAYLPGLWFKTGDRADVDENGWLRLRGRSKDIIIRGGENIPVTDVESAIFDHPDVLNAAVIGLPDERL GERVCAVLVTKSGCPELTVDTLGEYLLGQGLSKHYLPEKVVHLDELPMTPSGKIQKFKLREQYS*
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. smegmatis MC2 155 | MSMEG_4112 | - | - | 100% (555) | cyclohexanecarboxylate-CoA ligase |
| M. smegmatis MC2 155 | MSMEG_4516 | - | 3e-62 | 32.35% (541) | 2,3-dihydroxybenzoate-AMP ligase |
| M. smegmatis MC2 155 | MSMEG_5649 | - | 2e-60 | 33.92% (513) | acyl-CoA synthetase |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb2405 | mbtA | 2e-60 | 30.80% (539) | bifunctional salicyl-AMP ligase/salicyl-S-arcp synthetase |
| M. gilvum PYR-GCK | Mflv_2691 | - | 7e-59 | 30.90% (547) | AMP-dependent synthetase and ligase |
| M. tuberculosis H37Rv | Rv2384 | mbtA | 2e-60 | 30.80% (539) | bifunctional salicyl-AMP ligase/salicyl-S-arcp synthetase |
| M. leprae Br4923 | MLBr_01051 | xclC | 2e-42 | 33.71% (356) | acyl-CoA synthetase |
| M. abscessus ATCC 19977 | MAB_4377c | - | 1e-54 | 31.40% (516) | AMP-binding domain protein |
| M. marinum M | MMAR_0303 | - | 2e-60 | 32.43% (555) | acyl-CoA synthetase |
| M. avium 104 | MAV_2638 | - | 8e-68 | 34.71% (533) | AMP-dependent synthetase and ligase |
| M. thermoresistible (build 8) | TH_2907 | - | 3e-62 | 32.68% (563) | PUTATIVE AMP-dependent synthetase and ligase |
| M. ulcerans Agy99 | MUL_3641 | mbtA | 6e-58 | 31.07% (544) | bifunctional enzyme MbtA: salicyl-AMP ligase (SAL-AMP |
| M. vanbaalenii PYR-1 | Mvan_3850 | - | 2e-60 | 31.47% (556) | AMP-dependent synthetase and ligase |
CLUSTAL 2.0.9 multiple sequence alignment
Mb2405|M.bovis_AF2122/97 --MPPKAADGRRPSPDGGLGGFVPFPA-DRAASYRAAGYWSGRTLDTVLS
Rv2384|M.tuberculosis_H37Rv --MPPKAADGRRPSPDGGLGGFVPFPA-DRAASYRAAGYWSGRTLDTVLS
MUL_3641|M.ulcerans_Agy99 ------------------MAGFIPYPP-ERVASYRAAGYWTGRSIGSLLR
Mflv_2691|M.gilvum_PYR-GCK MTTGFPIEYGHGLTNGDLKHGFVPFPE-DRAEAYRRAGYWTGRPLDSILT
Mvan_3850|M.vanbaalenii_PYR-1 MNTGLPADQGN-----DLTRGFVPFPP-DRAEVYRRAGYWTGRPLDSILT
TH_2907|M.thermoresistible__bu MTTEFQPVESDLTS--DFRTGFVPFPP-DRVEEYRRAGYWTGRRLDSILS
MSMEG_4112|M.smegmatis_MC2_155 -----MTAPTERRLANVLNGQYTPIDD-DTAANWRAAGWWENRSIRSLLA
MMAR_0303|M.marinum_M ------MGGPKVPQRRLIDGRWVRWDA-DRAAGAYAHRWWVRRTLADALR
MAV_2638|M.avium_104 ----------------------MPFDHRQLRDKWYSAGWYSDRTCLDAFE
MAB_4377c|M.abscessus_ATCC_199 ---------------------------MKAYDAGATLSPIIEETIGENFE
MLBr_01051|M.leprae_Br4923 -------------------------------------------MLLASLN
:
Mb2405|M.bovis_AF2122/97 DAARRWPDRLAVADAGDRPGHGGLSYAELDQRADRAAAALHGLGITPGDR
Rv2384|M.tuberculosis_H37Rv DAARRWPDRLAVADAGDRPGHGGLSYAELDQRADRAAAALHGLGITPGDR
MUL_3641|M.ulcerans_Agy99 QAAARWPDRVGVVDAG-----ASHTFCELDKLADRVAAGFVSLGIAPGDR
Mflv_2691|M.gilvum_PYR-GCK DAASRWPDKPAIIDAT-----RSYTFAELDAAADRAAAGLADLGIRPGER
Mvan_3850|M.vanbaalenii_PYR-1 EAAATWPDKPAVIDSA-----QTYTFRELDAAADRVAAGLAALGIRPGDR
TH_2907|M.thermoresistible__bu DAAAAQPDKAAVIDPD-----RSHTFAELDALADRVAAGLADLGIAPGDR
MSMEG_4112|M.smegmatis_MC2_155 DAAQAHPDRIALVGRRADGGRVARTYQEFDRNANQVASVLASLGVRPDDA
MMAR_0303|M.marinum_M EAAQSTPQRVALVDAD-----RQLDCQELHRQAMALAQHLRSR-MPAGSV
MAV_2638|M.avium_104 AGAVEHKDVPLTFVIG--DSVSSPTVCEIHDRAVALAASLQRLGVRDGDA
MAB_4377c|M.abscessus_ATCC_199 RIARAHPDVGALVDVS---GDRRWTYRELDAEINRVARGLMSLGVAAGDR
MLBr_01051|M.leprae_Br4923 PSVVTATDIADAVCVDG---------VVLSRSGLVGAATSVAERVGGARL
. : : * :
Mb2405|M.bovis_AF2122/97 VLLQLPNGCQFAVALFALLRAGAIPVMCLPGHRAAELGHFAAVSAATGLV
Rv2384|M.tuberculosis_H37Rv VLLQLPNGCQFAVALFALLRAGAIPVMCLPGHRAAELGHFAAVSAATGLV
MUL_3641|M.ulcerans_Agy99 VLLQLPNSCRFAVALFGLLRAGAIPVMCLPGHRLAELHHFAEVSGAVGLI
Mflv_2691|M.gilvum_PYR-GCK VLLQLPNSCQFAVALFALLRAGVVPVMCLTGHRTAELGHFADVSGAVAVI
Mvan_3850|M.vanbaalenii_PYR-1 VLLQLPNSCQFAIALFGLLRAAAVPVMCLPGHRTAELGHFAEVSGAVALI
TH_2907|M.thermoresistible__bu VLLQMPNDTRFAVAFFGLLRAGAIPVMCLPGHRSAEMSHFAEVSGAVGLI
MSMEG_4112|M.smegmatis_MC2_155 VVVMLPNWVEYPEFLFGINELGAIYAGIPVAYGDQQAAAILRRSRARVLV
MMAR_0303|M.marinum_M VSFMLPNWHEAAVIYLASTLAAMVANPVLPSLREHELRFILEDAGSRMIF
MAV_2638|M.avium_104 VAVQLTNRVECAVAYQAVLLCGAVLVPIVHIYGMAEVAFVLDQSAAKVLI
MAB_4377c|M.abscessus_ATCC_199 VGIWAPNCAEWVLVQYATAKIGAILVNINPAYRTHELAYALNQSGVRTLI
MLBr_01051|M.leprae_Br4923 VAVLATPTASTVLAITGCLIAGVPVVPVPADIGVVERRHMLTDSGAQAWL
* . . . . : : .
Mb2405|M.bovis_AF2122/97 VADVASGFDYRPMARELVADHPTLRHVIVDGDPGP-----FVSWAQLCAQ
Rv2384|M.tuberculosis_H37Rv VADVASGFDYRPMARELVADHPTLRHVIVDGDPGP-----FVSWAQLCAQ
MUL_3641|M.ulcerans_Agy99 VAQHAGGFDYRSMAERLVAAHPQLRHVIIDGESGP-----FLPWSSLSEG
Mflv_2691|M.gilvum_PYR-GCK VPDRAGGFDYRDMAASLAAQRPALRHVIVDGEPGD-----QVSWQSILDF
Mvan_3850|M.vanbaalenii_PYR-1 VPDEAGGFDYRDMAGRLAAEHPSLQHVIVDGENGP-----FVAWSSIRDF
TH_2907|M.thermoresistible__bu IPERANGFDYRELARALTASRPRLRHVIVDGATDPSG-DGFVPWSALAEF
MSMEG_4112|M.smegmatis_MC2_155 IPRRWRGNNILEQSRRLRDQIPTLQQVIVLDDDGTDLRDGESLWSDHAHV
MMAR_0303|M.marinum_M APADFRGHDYTSMLRRVAAQLPSRPEVIEVRGQCPDG---VSTFQSLLVG
MAV_2638|M.avium_104 MPERHGSIVYSKRLQDLSRIDSLQHVVMLDAEPGEG----YLAWSQLDTP
MAB_4377c|M.abscessus_ATCC_199 CAKAFKSSDYVAMASQVMPDAPGLRDVVFIGTSDWT---ELVAGAERVTE
MLBr_01051|M.leprae_Br4923 GPGPSADSAPDG-----------------------------LPHIPVQLD
. .
Mb2405|M.bovis_AF2122/97 AGTGSPAPPADPGSPALLLVSGGTTGMPKLIPRTHDDYVFNATASAALC-
Rv2384|M.tuberculosis_H37Rv AGTGSPAPPADPGSPALLLVSGGTTGMPKLIPRTHDDYVFNATASAALC-
MUL_3641|M.ulcerans_Agy99 A---TPDIDVDTTSPALLLVSGGTTGTPKLIPRTHDDYVYNATASAQLC-
Mflv_2691|M.gilvum_PYR-GCK AGPRLGRTPVDPSLPALLLVSGGTTGLPKLIARTHDDYVYNAHACAQAC-
Mvan_3850|M.vanbaalenii_PYR-1 DGPAVERAAVDPGLPALLLVSGGTTGLPKLIARTHDDYVYNAVASAQAC-
TH_2907|M.thermoresistible__bu AGPAVPRPPVDTTAPALLLVSGGTTGLPKLIPRTHDDYVYTAVASARAY-
MSMEG_4112|M.smegmatis_MC2_155 AARQFPPP--DPGQICYLGFTSGTTGEPKGAMHSHNTLIYSARRQAEHIG
MMAR_0303|M.marinum_M RETAAPLPTADPDAVRMVLYTSGTTGRPKGVLHTHNSIHALICQIRDHW-
MAV_2638|M.avium_104 AAR-YRRPAVAADDVCLLLYTSGTTSAPKGVQHTNNTVLAEQRTMPALLA
MAB_4377c|M.abscessus_ATCC_199 TALRARMSQLSNTDPINIQYTSGTTGYPKGATLSHRNVLNNGFFVAESI-
MLBr_01051|M.leprae_Br4923 ARSWNRYPEPSPDATAMVIYTSGTTGPPKGVLLSRRAIAVDLDALAQAW-
: :.***. ** :.
Mb2405|M.bovis_AF2122/97 --RLSADDVYLVVLAAGHNFPLACPGLLGAMTVGATAVFAP-DPSPEAAF
Rv2384|M.tuberculosis_H37Rv --RLSADDVYLVVLAAGHNFPLACPGLLGAMTVGATAVFAP-DPSPEAAF
MUL_3641|M.ulcerans_Agy99 --RLTADDVYLVSLPAAHNFPLACPGILGSMAVGATIVFST-DPSPEAAF
Mflv_2691|M.gilvum_PYR-GCK --EMTGEDAYLVALPAGHNFPLACPGLLGSMTVGAPTVFTA-DPSPENAF
Mvan_3850|M.vanbaalenii_PYR-1 --EMTAEDAYLVALPAGHNFPLACPGMLGSMTVGAPTVFTA-DPSPENAF
TH_2907|M.thermoresistible__bu --EMTSDDVYLVVLPAAHNFPLACPGMLGAMTVGASTVFTA-DPSPEAAF
MSMEG_4112|M.smegmatis_MC2_155 TEAFGEPVVNLVASPMGHHTGYVWGGVFTVMLAGTAVHVDR-WDPTWGAQ
MMAR_0303|M.marinum_M --MVEPGDGFLVASPIAHIGGSIYAFECPLLLGTTAVLLDR-WDADRAVQ
MAV_2638|M.avium_104 G---KPDDVSLVTFPPGHIAGVGS-MLRPLLSGARTVFMDG-WDPARAVE
MAB_4377c|M.abscessus_ATCC_199 --QLQAGDRLCIPVPFYHCFGMVMGNLGCTTHGATIVMPAPGFDPGRTLE
MLBr_01051|M.leprae_Br4923 --QWTADDVLVHGLPLFHVHGLVLGLLGSLRIGNRFVHTGK-PTPTAYAQ
. * .
Mb2405|M.bovis_AF2122/97 AAIERHGVTVTALVPALAKLWAQSCEWEPVTPKSLRLLQVGGSKLEPEDA
Rv2384|M.tuberculosis_H37Rv AAIERHGVTVTALVPALAKLWAQSCEWEPVTPKSLRLLQVGGSKLEPEDA
MUL_3641|M.ulcerans_Agy99 TLIERHGVTVTALVPALAKLWTQACDWEPVIPKSLRLLQVGGSRLEPEDA
Mflv_2691|M.gilvum_PYR-GCK ALIDKHRITVTGLVNALAKLWAQACDWEPVLPTSLRFVQVGGSRMSPEEA
Mvan_3850|M.vanbaalenii_PYR-1 ALIDKFRITVTGLVNALAKLWAQACEWEPVLPTSLRFVQVGGSRMSPEEA
TH_2907|M.thermoresistible__bu PLINRHRVTVTGLVNALAKLWAQACEWEPELPESLRVVQVGGSRMSPQEA
MSMEG_4112|M.smegmatis_MC2_155 VVREEGVTTFFGAPTFLQDIIRTELAGDPACP--LRCMVVAGAPVPRNLP
MMAR_0303|M.marinum_M LMTSKRCTHMAGATPFLEQLLAAAQRAGTRLP-DLKFFVCGGASVSPSLI
MAV_2638|M.avium_104 VVHRFGVTCTAGTPFHLAGILDLGDTGDKLAS--LREFLVGAAPVAEEIG
MAB_4377c|M.abscessus_ATCC_199 AIERERCVGVYGVPTMFIAMLADPGFAHRDLSTLRTGIMAGSVCPVEVMK
MLBr_01051|M.leprae_Br4923 ACSEAGGSLYFGVPTVWSRLVADEAVARALRP--ARLLVSGSASLPVPVF
. : . . ..
Mb2405|M.bovis_AF2122/97 RRVRTALTPGLQQVFGMAEGLLNFTR--IGDPPEVVEHTQGRPLCPADEL
Rv2384|M.tuberculosis_H37Rv RRVRTALTPGLQQVFGMAEGLLNFTR--IGDPPEVVEHTQGRPLCPADEL
MUL_3641|M.ulcerans_Agy99 RQAREALTPGLQQVFGMAEGLLNFTR--LEDPPDLLEHTQGRPLCSADEL
Mflv_2691|M.gilvum_PYR-GCK RFILDRLTPGMSQIFGMAEGMLNFTR--PGDPEDVVVHTQGRPMSPHDEM
Mvan_3850|M.vanbaalenii_PYR-1 RFILERLTPGMSQIFGMAEGMLNFTR--PGDPEDVVVHTQGRPMSPHDEV
TH_2907|M.thermoresistible__bu QFILDRLTPGMSQIFGMAEGMLNFTR--PGDPIDVVVHTQGRPMSPHDEM
MSMEG_4112|M.smegmatis_MC2_155 AQAAEALGAYVAPAWGMTECSILTSC--TPDEPDAILRTDG-SVFAGSEV
MMAR_0303|M.marinum_M RCATDYFDK------------AVVTR--VYGSTEVPVTTIGAPDSSAHAA
MAV_2638|M.avium_104 RRAAQAGISTFRSYGATEHPTVTGEH--EGQPQWARLGTDGKPLPGS-EI
MAB_4377c|M.abscessus_ATCC_199 RCIDEMHMAGVAIAYGMTETSPVSCQTLFDDDLDRRTATVGRAHPHIEIK
MLBr_01051|M.leprae_Br4923 DRLAHLTGHRPIERYGSTESLITLST---LADGERRAGWVGLPLAGVQTR
* .
Mb2405|M.bovis_AF2122/97 RIVNADGEPVGPGE-EGELLVRGPYTLNGYFAAERDNERCFDPDGFYRSG
Rv2384|M.tuberculosis_H37Rv RIVNADGEPVGPGE-EGELLVRGPYTLNGYFAAERDNERCFDPDGFYRSG
MUL_3641|M.ulcerans_Agy99 RIVDGAGAPVAPGA-EGELLVRGPYTINGYFCADRDNERCFDPAGFYRSG
Mflv_2691|M.gilvum_PYR-GCK RVVDEDGVEVAPGE-EGELLVRGPYTLNGYYRADDANARSFTPDGFYRTG
Mvan_3850|M.vanbaalenii_PYR-1 RVVDESGDGVPPGE-EGELLVRGPYTLYGYYRDDDANARSFSPDGFYRTG
TH_2907|M.thermoresistible__bu RVVDESGAEVAPGE-EGELLVRGPNTLNGYYRDDEANARSFSPDGFYRTG
MSMEG_4112|M.smegmatis_MC2_155 KIVDDTGAAVAAGV-VGDLLMRGPGVVYGYYDRPDATRDAYLPGLWFKTG
MMAR_0303|M.marinum_M ATDGRVGAADVKLV-AGEIRVRGPQMLVGYRHPEDEAG-SFDAQGYFRTG
MAV_2638|M.avium_104 RIVGSDGTDCPIGI-GGEVVVRGPDQFIGYQDS-ALNTEAFTDDGWFRTG
MAB_4377c|M.abscessus_ATCC_199 IVDPNSGETVQRGQ-SGELCTRGYSVMLGYWNDEAHTREVLDTDGWMHTG
MLBr_01051|M.leprae_Br4923 LVDESGGPVPYDGETVGRLQVRSPTMFGGYLNRPEATAEAFDEDGWYRTG
* * : *. . ** : ::*
Mb2405|M.bovis_AF2122/97 DLVRRRDDG----NLVVTGR-VKDVICRAGETIAASDLEEQLLSHPAIFS
Rv2384|M.tuberculosis_H37Rv DLVRRRDDG----NLVVTGR-VKDVICRAGETIAASDLEEQLLSHPAIFS
MUL_3641|M.ulcerans_Agy99 DLVRLRDDG----YLQVTGR-VKDVICRCGETISAQDLEEQLLSHPAIRS
Mflv_2691|M.gilvum_PYR-GCK DRVRIFTDGPRAGYVEVTGR-VKDVIHRGGETVSATDLEDHLHTHPSVYA
Mvan_3850|M.vanbaalenii_PYR-1 DRVRIFADGPRAGYVEVVGR-IKDVIHRGGETVSATDLEDHLHTHPAIYA
TH_2907|M.thermoresistible__bu DRVRIFADGPRAGHVEVTGR-VKDVIHRGGETVSATDLEDHLLTHPAIYA
MSMEG_4112|M.smegmatis_MC2_155 DRADVDENG----WLRLRGR-SKDIIIRGGENIPVTDVESAIFDHPDVLN
MMAR_0303|M.marinum_M DLGHWVDDE----YLVVTGR-AKDIIIRNGENISPKEIEDILITHPGIAD
MAV_2638|M.avium_104 DLGHLDAHG----RLTITDR-IKDVIIRGGETISSGQVEEVLNAHPAVAD
MAB_4377c|M.abscessus_ATCC_199 DLAVMRDDG----YCTIIGR-LKDMVIRGGENIYPREIEEFLLTHPDIED
MLBr_01051|M.leprae_Br4923 DVAVVDSGG----MHRIVGRESVDLIKLGGYRIGAGEIEMALLGHPDVRE
* : .* *:: * : ::* : ** :
Mb2405|M.bovis_AF2122/97 AAAVGLPDQYLGEKICAAVVFAG---APITLAELNGYLDRRGVAAHTRPD
Rv2384|M.tuberculosis_H37Rv AAAVGLPDQYLGEKICAAVVFAG---APITLAELNGYLDRRGVAAHTRPD
MUL_3641|M.ulcerans_Agy99 AAAVPLPDPDMGELICAAIVFAGPPQTPQTLSGLNDYLEQRGVAAHIRID
Mflv_2691|M.gilvum_PYR-GCK AAAVALPDEYLGEKICAAVVFTG---PPLTLADLNAFLDERGASTHARPD
Mvan_3850|M.vanbaalenii_PYR-1 AAAVALPDEYLGEKICAAVVFRG---KPITLAELNAFLDERGASKHARPD
TH_2907|M.thermoresistible__bu AAAVPLPDEFLGEKICAAVVFRG---APVSLTELNGYLDERGVATHARPD
MSMEG_4112|M.smegmatis_MC2_155 AAVIGLPDERLGERVCAVLVTKSG-CPELTVDTLGEYLLGQGLSKHYLPE
MMAR_0303|M.marinum_M VAIVGLPDDRTGERACAVIVAAAD--PPPGLPGVRAFLEGAGVARFKIPE
MAV_2638|M.avium_104 GVAVAAPDSRYGDVVAAVVVLKPG--YALNLDDLRAHFAASGLARQKTPE
MAB_4377c|M.abscessus_ATCC_199 VHVVGVPDEKYGEELCAWVRMRPD-RVVIDAVAIRAFASGR-LAHYKIPR
MLBr_01051|M.leprae_Br4923 VAVVGLPDEDLGQRIVAFVVGAEA----LDADELINYVAQQ-LSIHKRPR
: ** *: * : : . :
Mb2405|M.bovis_AF2122/97 QLVAMPALPTTPIGKIDKRAIVRQLGIATGPVTTQRCH--
Rv2384|M.tuberculosis_H37Rv QLVAMPALPTTPIGKIDKRAIVRQLGIATGPVTTQRCH--
MUL_3641|M.ulcerans_Agy99 RLVAMPSLPTTPVGKIDKKAIASQILTTTAT---------
Mflv_2691|M.gilvum_PYR-GCK VLVPVPSLPTTAVGKVDKKALVAQLTANS-----------
Mvan_3850|M.vanbaalenii_PYR-1 VLMPVRSLPTTAVGKVDKKQLGAQLTS-------------
TH_2907|M.thermoresistible__bu VLVALPSLPMTAVGKVDKKKVVEQVLSQ------------
MSMEG_4112|M.smegmatis_MC2_155 KVVHLDELPMTPSGKIQKFKLREQYS--------------
MMAR_0303|M.marinum_M QVVIWDHLPKNHAGKIVKHRIKAALMKAEQ----------
MAV_2638|M.avium_104 RLAIVDELPRTSLGKVRKAQLRSDHFGAARR---------
MAB_4377c|M.abscessus_ATCC_199 YVHVVESFPMTVTGKVRKIEMRQQTIQIFGLPEPGRTDEQ
MLBr_01051|M.leprae_Br4923 EVRFVDALPRNAMGKVLKKQLLFEG---------------
: :* . **: * :