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ANHGRKHATLAALPGVRAVRRPVAPGADEQFDVFYVRAGRKSRHPVVLIPGGPGVASIQQYRGLRRRLAT SGLDVIMVEHRGIGMSRRTDAGADLPAEAITVEQVLDDIAAVLDDAGVGTAVVYGTSYGSYLASGVGVRH PGRVAAMVLDSPVLSAHDLDEMRTAIRNLLWEGTGPDTAALAPKVRKLVETGRLTGGQLAGLLYGYGGAR LLDRQLDLLLDGHTLLWRTLGRLGRVSTRKAPYRNEIDLVGRIAFRELNYAGVPDGLPLDPALEMAEMAR ATKFHEPFEAEPYDLTAEMPKFTWPTVVVSGGRDLITPPALAQRVASLIPGATPVCMATAAHSIVDTRER AAIAIMQAVCNGAAEQLPHTADELDALPPRMPIRLLVAAISAAVAAEGMADSVLPATS*
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. smegmatis MC2 155 | MSMEG_4998 | - | - | 100% (409) | hydrolase, alpha/beta fold family protein |
| M. smegmatis MC2 155 | MSMEG_6106 | - | 7e-07 | 30.88% (136) | epoxide hydrolase |
| M. smegmatis MC2 155 | MSMEG_0220 | - | 7e-07 | 29.56% (159) | monoglyceride lipase |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb0665c | lipG | 2e-05 | 28.93% (121) | lipase/esterase LipG |
| M. gilvum PYR-GCK | Mflv_2257 | - | 1e-125 | 58.33% (408) | alpha/beta hydrolase fold |
| M. tuberculosis H37Rv | Rv3617 | ephA | 2e-06 | 31.67% (120) | epoxide hydrolase EphA |
| M. leprae Br4923 | MLBr_01899 | lipG | 1e-06 | 26.20% (187) | putative hydrolase |
| M. abscessus ATCC 19977 | MAB_4366c | - | 2e-06 | 33.64% (107) | putative hydrolase, alpha/beta fold |
| M. marinum M | MMAR_4140 | - | 1e-135 | 60.64% (404) | hydrolase |
| M. avium 104 | MAV_1430 | - | 1e-133 | 62.00% (400) | hydrolase, alpha/beta fold family protein |
| M. thermoresistible (build 8) | TH_1861 | lipG | 1e-09 | 30.00% (190) | PROBABLE LIPASE/ESTERASE LIPG |
| M. ulcerans Agy99 | MUL_4193 | ephA | 8e-07 | 32.20% (118) | epoxide hydrolase EphA |
| M. vanbaalenii PYR-1 | Mvan_4434 | - | 1e-123 | 58.72% (407) | alpha/beta hydrolase fold |
CLUSTAL 2.0.9 multiple sequence alignment
Mb0665c|M.bovis_AF2122/97 ----------MDIRSGTA-VSG-----------------DVKLYYEDMGD
MLBr_01899|M.leprae_Br4923 ----------MEVRSGYA-TSG-----------------DLKLYYEDMGN
TH_1861|M.thermoresistible__bu ----------MQIRTGFANVDGS--------------PEPVDLYYEDLGD
MMAR_4140|M.marinum_M ---MTMSDKRRRAHEKLAGMPGVRPVRRPVSPG---NDSEFDLYYVRAGR
MAV_1430|M.avium_104 ---MTMNAKRRRMQQRLAKLPGVRAVRRPISPD---TADEFDLYYVRAGR
MSMEG_4998|M.smegmatis_MC2_155 ------MANHGRKHATLAALPGVRAVRRPVAPG---ADEQFDVFYVRAGR
Mflv_2257|M.gilvum_PYR-GCK MSVMTMNARRRRVHDRLAALPGVRPVRRPVHPG---ADGHFDVFYVRTGR
Mvan_4434|M.vanbaalenii_PYR-1 MRVMTMNGRRRRAHDKLAALPGVRPVRRPVRPGVGDADEKFDVYYVRTGR
Rv3617|M.tuberculosis_H37Rv ---------MGAPTERLVDTNG------------------VRLRVVEAGE
MUL_4193|M.ulcerans_Agy99 --------MVATSTERLVDTNG------------------VRLRVTEAGE
MAB_4366c|M.abscessus_ATCC_199 -------MTIHTHLQSISTTLG----------------EIAVHDVPAAGG
* *
Mb0665c|M.bovis_AF2122/97 LDHPPVLLIMG-LGAQMLLWRTDFCARLVAKGLRVIRYDNRDVGLSTKT-
MLBr_01899|M.leprae_Br4923 VDDPPVLLIMG-LGAQLVLWRTAFCEKLVAQGLRVVRYDNRDVGLSSRTD
TH_1861|M.thermoresistible__bu PADPPVLLIMG-LGAQLILWRDGFCRKLVERGLRVIRFDNRDVGLSSKL-
MMAR_4140|M.marinum_M KSAHPLVIIPGGPGVASMLVYKGLRRRAAAAGLDVIMIEHRGVGMSRHD-
MAV_1430|M.avium_104 KSRHPVVIIPGGPGVASVQMYKGLRRRAAAAGLDVIMIEHRGVGMSRHD-
MSMEG_4998|M.smegmatis_MC2_155 KSRHPVVLIPGGPGVASIQQYRGLRRRLATSGLDVIMVEHRGIGMSRRT-
Mflv_2257|M.gilvum_PYR-GCK KSAHPLVVIPGGPGAASIALYRSFRRHAAVAGLDVIMVEHRGVGLSRHD-
Mvan_4434|M.vanbaalenii_PYR-1 KSAHPLVIIPGGPGAASIALYRAFRRRAAVAGLDVIMVEHRGVGLSRHD-
Rv3617|M.tuberculosis_H37Rv PGAPVVILAHG--FPELAYSWRHQIPALADAGYHVLAPDQRGYGGSSRP-
MUL_4193|M.ulcerans_Agy99 RGAPVVILAHG--FPELAYSWRHQIPVLAEAGYHVLAPDQRGYGGSSRP-
MAB_4366c|M.abscessus_ATCC_199 ADAPTLVMLHGGGPGASGLSNYEQNIPALSRTFRILLPDQPGFGGSYRP-
::: * :: :: . * * :
Mb0665c|M.bovis_AF2122/97 --ERHRPGQPLATRLVRSWLGLPSQAAYTLEDMAADAAALLDHLDVKHAH
MLBr_01899|M.leprae_Br4923 STEQPCPSQPLTARLIRFWLGQRNACAYTLEDMTDDAVALLDHLSIERAH
TH_1861|M.thermoresistible__bu --DGRRERTPLPWRMLRSFAGLPSPAVYTLEDMADDAAGLLDHLQIDAAH
MMAR_4140|M.marinum_M -----DSGADLPPEAFTIDQVVDDIAAVLDDAHVDSAVIYGTSYGTYIAA
MAV_1430|M.avium_104 -----DAGADLPPEAMTVNQVVDDVAAVLDDARVDSAVVYGASYGTYIAS
MSMEG_4998|M.smegmatis_MC2_155 -----DAGADLPAEAITVEQVLDDIAAVLDDAGVGTAVVYGTSYGSYLAS
Mflv_2257|M.gilvum_PYR-GCK -----DDGADLPPEALTVDAAVGDIAAVLDDAGVERAVVYGASYGSYLAA
Mvan_4434|M.vanbaalenii_PYR-1 -----DSGADLPPEALTVDAAVDDIAAVLDDAQVERASVYGTSYGSYLAA
Rv3617|M.tuberculosis_H37Rv ----------EAIEAYDIHRLTADLVGLLDDVGAERAVWVGHDWGAVVVW
MUL_4193|M.ulcerans_Agy99 ----------EAIEDYDIHQLTADLVGLLDDVGAERAAWVGHDWGAVVVW
MAB_4366c|M.abscessus_ATCC_199 ------TGADLDERSITEIT-VDALFQVLDDLAVGSFHLLGNSLGGAAAI
. : . .
Mb0665c|M.bovis_AF2122/97 VVGASMGGMIAQIFAARFAQRTKTLAVIFSSNNHRFLPP-------PAPR
MLBr_01899|M.leprae_Br4923 IVGASMGGMIAQIFAARFPTRTRSLAVFFSSNNRPFLPP-------PAPR
TH_1861|M.thermoresistible__bu IVGASMGGMVAQVFAGRHAHRTRSLGVIFSSNNRPFLPP-------PGPR
MMAR_4140|M.marinum_M GVGVRHPARVRSMILDSPLLSRDDIALARETIRRRLLDGDSPETAALAAK
MAV_1430|M.avium_104 GVGVRHPARVHGMVLDSPLLSRHDIVIVRRAIRRLLLRGDSPETASLAPK
MSMEG_4998|M.smegmatis_MC2_155 GVGVRHPGRVAAMVLDSPVLSAHDLDEMRTAIRNLLWEGTGPDTAALAPK
Mflv_2257|M.gilvum_PYR-GCK GVGVRHPGRIHAMVLDSPLLSAHDIDAVRAQTRRVLWED-----TDLAPR
Mvan_4434|M.vanbaalenii_PYR-1 GVGVRHPGRVQSMVLDSPLLSADDIDAVRAQTRRVLWDG-----ADLAPR
Rv3617|M.tuberculosis_H37Rv NAPLLHADRVAAVAALSVPALPRAQVPPTQAFRSRFGENFFYILYFQEPG
MUL_4193|M.ulcerans_Agy99 NAPLLHPDRVAAVAGLSVPVLPRAQVPPIQAFRQRFGEHFFYILYFQQPG
MAB_4366c|M.abscessus_ATCC_199 RMAQLRPERVTRLVLMAPGGGWLPFGPTPTE----------------GQK
: :
Mb0665c|M.bovis_AF2122/97 ALLALLTGPPPD--------------------------SPRDVIVDNAVR
MLBr_01899|M.leprae_Br4923 ALLALLTGPPTG--------------------------SRRDVVVDNVVR
TH_1861|M.thermoresistible__bu QLLSIITGPAPD--------------------------APRDEVIENAVK
MMAR_4140|M.marinum_M VRQLVDAGVMTARASQVAAAVYGYGGARLLERQLDLLLCGRMILWHVLFR
MAV_1430|M.avium_104 MRKLVDAGLMTATATQVVATIYGYGGARLLERQLDLLLDGRKLLWWTLSR
MSMEG_4998|M.smegmatis_MC2_155 VRKLVETGRLTG--GQLAGLLYGYGGARLLDRQLDLLLDGHTLLWRTLGR
Mflv_2257|M.gilvum_PYR-GCK VRTLADEGALTPQDVLLMIGLYELAGPQVLRRQLDLRVADRNRLWKIAER
Mvan_4434|M.vanbaalenii_PYR-1 MRRLAADGVLRSEDVLLAIGLYELAGPEGLGRQLDLLLARRDWLWRVAES
Rv3617|M.tuberculosis_H37Rv IADAELNGDPARTMRRMIGGLRP--------------PGDQSAAMRMLAP
MUL_4193|M.ulcerans_Agy99 VADAELNGDPARTMRRMIGGQRS--------------PTDQGAALRMVAP
MAB_4366c|M.abscessus_ATCC_199 EMFRYFNGEGPT--------------------------EKKMAAFIRAMV
* :
Mb0665c|M.bovis_AF2122/97 VSKII--GSPAYPIPEDQVRAEAAESYDRNFHPWG----IAQQFSAILGS
MLBr_01899|M.leprae_Br4923 VTKIT--GSPLYRMPEEQVRTNAAEIYDRSFYPLG----VSRQFSAILGS
TH_1861|M.thermoresistible__bu VGRLN--GSPAYPVPDEQLRADAATAFDRSYHPVG----VARQFDAILGS
MMAR_4140|M.marinum_M FSAMY-ARKVPYRYEMDLVGRIAFRELDYAAEPDGLPLDPAQAIRETIAA
MAV_1430|M.avium_104 FTTL---RNLPFRYEEDLVSRIALRELDYAAEPDGLPLDPAVAEREVRTQ
MSMEG_4998|M.smegmatis_MC2_155 LGRVS-TRKAPYRNEIDLVGRIAFRELNYAGVPDGLPLDPALEMAEMARA
Mflv_2257|M.gilvum_PYR-GCK VTRLVTERKTPYHHEPDLVERIAYQELNYGAVPDGGPVDPAVALREMASG
Mvan_4434|M.vanbaalenii_PYR-1 VTKLVTERKTAYRHEPDLVERIAYRELNYGAVPDGGPLDPSVALRQMATG
Rv3617|M.tuberculosis_H37Rv GPDGFIDRLPEPAGLPAWISQEELDHYIGEFTRTG--FTGGLNWYRNFDR
MUL_4193|M.ulcerans_Agy99 GPEGFIDRLPEPDAPPSWISRDELDHYISEFTRTG--FTGGLNWYRNFDR
MAB_4366c|M.abscessus_ATCC_199 FDHKQFGRDVVSRRYQASLDEGHIEFYHHYN----------AAFAKRNGM
:
Mb0665c|M.bovis_AF2122/97 G-----------SLLRYDRRIVAPTVVIHGRADKLMRPFGGRAVARAING
MLBr_01899|M.leprae_Br4923 G-----------SLLHYNQRIIAPTVVIHGRADKLVRPSSGRAVARAITG
TH_1861|M.thermoresistible__bu G-----------SLRRYNRRTTSPTVVIHGRADKLMRPTGGRAVARAIPG
MMAR_4140|M.marinum_M T---DQFESEPYDLVAEMPRFGWPTVVVSGGHDLTTPAMVAEHIASLLPN
MAV_1430|M.avium_104 T---ATFEDEPYDLPAEMPSFDWPTAVVSGGRDLITPPAVAERVASLLPH
MSMEG_4998|M.smegmatis_MC2_155 TKFHEPFEAEPYDLTAEMPKFTWPTVVVSGGRDLITPPALAQRVASLIPG
Mflv_2257|M.gilvum_PYR-GCK T---VEFVGEPYDLPAAMPGFGWPTAVLSGGRDLTTPPEVARRVADLIPD
Mvan_4434|M.vanbaalenii_PYR-1 D---VDFVAEPYDLTSEMPGFAWPTVVVSGGRDLTTPPEVARRIADLIPD
Rv3617|M.tuberculosis_H37Rv N--------WETTADLAGKTISVPSLFIAGTADPVLTFTRTDRAAEVISG
MUL_4193|M.ulcerans_Agy99 N--------WETTADLEGATITVPSLFMAGTKDPVLSFARSDRASEVITG
MAB_4366c|M.abscessus_ATCC_199 D-----------PLWRDLHTITAPTLLLWGRDDRTITLEGAQMMLKHISD
*: .: * * :
Mb0665c|M.bovis_AF2122/97 -ARLVLIDGMGHDLPRQLWDRVIGELTRNFSEAG----------------
MLBr_01899|M.leprae_Br4923 -ARLVLFDGMGHDLPQQLWDQAVGVLMSNFAKAS----------------
TH_1861|M.thermoresistible__bu -ARLTLFDGMGHDLPEPLWDEIVAELDKNITAGR----------------
MMAR_4140|M.marinum_M -AVLIELPTMAHSALDFREPAALAIAGAVCRGEFGALTGRGEELDAMPAR
MAV_1430|M.avium_104 -AVLLSLPDMAHSALDFHEAVALASADAVLRGDLAGLAARACTLNALPPR
MSMEG_4998|M.smegmatis_MC2_155 -ATPVCMATAAHSIVDTRERAAIAIMQAVCNGAAEQLPHTADELDALPPR
Mflv_2257|M.gilvum_PYR-GCK -SVLVSLPTAGHSVLDIRERAAVEICKAVCSGELHELPSRAAELDALPAN
Mvan_4434|M.vanbaalenii_PYR-1 -AALLELPTAGHSILDTREHAALRICEAVQAGQSHDLPSRSTELDALPAN
Rv3617|M.tuberculosis_H37Rv PYREVLIDGAGHWLQQERPGEVTAALLEFLTGLELR--------------
MUL_4193|M.ulcerans_Agy99 PYREVMVEGAGHWLQQERPDEVNATLLDFLKGVQW---------------
MAB_4366c|M.abscessus_ATCC_199 -VQLHVFGRCGHWVQLERQAEFDRLVADFLGEPA----------------
. .*
Mb0665c|M.bovis_AF2122/97 ----------------------------------
MLBr_01899|M.leprae_Br4923 ----------------------------------
TH_1861|M.thermoresistible__bu ----------------------------------
MMAR_4140|M.marinum_M LPIRALWKAIELAAMAEHALPAAPLLHRRVSSA-
MAV_1430|M.avium_104 PEVRLLWALIAAAAMIEGALPIPAPLRRRLSCG-
MSMEG_4998|M.smegmatis_MC2_155 MPIRLLVAAISAAVAAEGMADSVLPATS------
Mflv_2257|M.gilvum_PYR-GCK LTVRLLVGAITVAARAESAVPGSAP----TAPTS
Mvan_4434|M.vanbaalenii_PYR-1 LTVRLLVRAITVAARAESAVPGAIPRVVRQLATS
Rv3617|M.tuberculosis_H37Rv ----------------------------------
MUL_4193|M.ulcerans_Agy99 ----------------------------------
MAB_4366c|M.abscessus_ATCC_199 ----------------------------------