For questions or suggestions e-mail us at: ioerger@cs.tamu.edu
VSSPTKPLVAEDAEIVRRVLAHIDAGTTDEGEAWREPVENYVSPERFAAELRLLRGMPSVFVPSAAIPNK GDHVERTAFGVPLFAVRGADGRARVFRNACRHRGFALVENAGCSHALVCRYHGWIYRLDGSLSHVPHAEA FPDLDMSARGLIEVESHEVDGLIVIGPLDPVDAGLIEDEGAIGWLTDGSPWRAKLVPAERLIVVESTLRE INWKVLVEQFLEGYHIRSTHKDTFFPVQYDDLNVVEAFGPNTRITFPYRNIERLRDRPEHTWTTGARVTY LYQLFPNVMLATFPDVIAMVVIDPVSVDRTAVTIYSMVTPDVAERDSLDASSNLAGAGSFIGQGLSEDNE MSEGVQRGLAAGANAFVEFGRHESAVGRFHAALGRRLARLTSVG
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. marinum M | MMAR_0230 | - | - | 100% (394) | dioxygenase |
| M. marinum M | MMAR_3964 | - | 3e-33 | 26.43% (367) | ring-hydroxylating dioxygenase, large terminal subunit |
| M. marinum M | MMAR_4744 | - | 3e-19 | 31.88% (207) | dioxygenase |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb3186c | - | 2e-16 | 26.44% (382) | dioxygenase |
| M. gilvum PYR-GCK | Mflv_2464 | - | 1e-15 | 28.57% (203) | Rieske (2Fe-2S) domain-containing protein |
| M. tuberculosis H37Rv | Rv3161c | - | 1e-16 | 26.44% (382) | dioxygenase |
| M. leprae Br4923 | - | - | - | - | - |
| M. abscessus ATCC 19977 | MAB_4370c | - | 6e-16 | 25.07% (347) | putative large terminal subunit of phenylpropionate dioxygenase |
| M. avium 104 | MAV_1644 | - | 3e-35 | 26.15% (390) | Rieske (2Fe-2S) domain-containing protein |
| M. smegmatis MC2 155 | MSMEG_0657 | - | 3e-22 | 29.74% (343) | Rieske (2Fe-2S) domain-containing protein |
| M. thermoresistible (build 8) | TH_4380 | - | 2e-16 | 28.62% (283) | PUTATIVE putative Rieske (2Fe-2S) domain protein |
| M. ulcerans Agy99 | MUL_4884 | - | 0.0 | 98.48% (394) | dioxygenase |
| M. vanbaalenii PYR-1 | Mvan_4910 | - | 1e-32 | 31.15% (305) | Rieske (2Fe-2S) domain-containing protein |
CLUSTAL 2.0.9 multiple sequence alignment
Mb3186c|M.bovis_AF2122/97 -------MLSTDNRAELGDILTDIGDYLDDNPP---ALSLPPAAYTSSEL
Rv3161c|M.tuberculosis_H37Rv -------MLSTDNRAELGDILTDIGDYLDDNPP---ALSLPPAAYTSSEL
MMAR_0230|M.marinum_M -MSSPTKPLVAEDAEIVRRVLAHIDAGTTDEGE---AWREPVENYVSPER
MUL_4884|M.ulcerans_Agy99 -MSSPTKPLVAEDAEIVRRVLAHIDAGTTDEGE---AWREPVENYASPER
MAV_1644|M.avium_104 -------MQHDQLIDLTRRALKLARDRTTDLAPT--AHVVDARDYTCVQR
Mvan_4910|M.vanbaalenii_PYR-1 MTAIADGPVSNQDEELTRRALRHAVDKTTDMADR--ELRVPLHYYRDPKI
MSMEG_0657|M.smegmatis_MC2_155 MDHFPAADYPDCMFKQIGPAPVPADDLAAALAPFGHSRMLPREAYVDPAV
MAB_4370c|M.abscessus_ATCC_199 -MASLPRPVAVAQGALCRPQALVRDIDVSGLVDS-HGGLLDRVIFTDGEL
Mflv_2464|M.gilvum_PYR-GCK ----------------MARFPKPPEGSWTEHYPQLGTGPVSYEDSIDPEF
TH_4380|M.thermoresistible__bu --------------------MTDLTAQRVPQKYACGETFVPKSRYIDPEF
Mb3186c|M.bovis_AF2122/97 WQLERERIFNRSWMLVAHVDQLAKTGDYVTVSVAGE--PVMVVRDVDGQL
Rv3161c|M.tuberculosis_H37Rv WQLERERIFNRSWMLVAHVDQVAKTGDYVTVSVAGE--PVMVVRDVDGQL
MMAR_0230|M.marinum_M FAAELR-LLRGMPSVFVPSAAIPNKGDHVERTAFGV--PLFAVRGADGRA
MUL_4884|M.ulcerans_Agy99 FAAELR-LLRGMPSVFVPSAAIPNNGDHVERTAFGV--PLFAVRGADGRA
MAV_1644|M.avium_104 HQQDRAMLLA-SPQLVGYVSELPAPGTYCTKTVMGR--SVLLTRTSDGSV
Mvan_4910|M.vanbaalenii_PYR-1 TEIEESQVLRRVPLAIVPSAQIPAVNDYVVRSVLGD--SLLVTRDRDGVS
MSMEG_0657|M.smegmatis_MC2_155 FAWEQQAIFS-GWTCVGHAADLETPGAQRAVGSGAN--GVLLVRGEDNRV
MAB_4370c|M.abscessus_ATCC_199 YRQELRRVFAPSWLFLAHADQFHRPGDFFTTYMGED--PVIVSMDKNRRI
Mflv_2464|M.gilvum_PYR-GCK YELERKAVFRRAWLNVGRVEQVPRKGSYFTKEMKVVNTSIIVVRTTSGEV
TH_4380|M.thermoresistible__bu LQLELDRLFTQVWQPACREEEIPDPGSYYEYCIGRQ--SIMVVRQKDRSI
: :: . . :: .
Mb3186c|M.bovis_AF2122/97 HALSPICRHR------LMLMVEPGAGRIDTLTCQYHLWRYGLDGRLRGAP
Rv3161c|M.tuberculosis_H37Rv HALSPICRHR------LMLMVEPGAGRIDTLTCQYHLWRYGLDGRLRGAP
MMAR_0230|M.marinum_M RVFRNACRHR------GFALVEN-AGCSHALVCRYHGWIYRLDGSLSHVP
MUL_4884|M.ulcerans_Agy99 RVFRNARRHR------GFALVEN-AGCSHALVCRYHGWIYRLDGSLSHVP
MAV_1644|M.avium_104 KAFNNVCLHR------QSQVATG-CGTASRFSCPYHAWTYDNTGRLVGLP
Mvan_4910|M.vanbaalenii_PYR-1 HVFLNYCRHR------GAKPACG-AGNTSRFTCPYHAWSYKNTGELFTVP
MSMEG_0657|M.smegmatis_MC2_155 RAFANACRHRG-----HELLSCGASAKRRSIVCPYHAWSYRLDGSVRNAP
MAB_4370c|M.abscessus_ATCC_199 RAFLNSCRHR------GARVCRADYGVTRGFTCTYHGWAYDLEGKLISVP
Mflv_2464|M.gilvum_PYR-GCK KAYHNICRHRGNKLVWNDMPLEETSGVCRQFTCKYHAWRYDLDGNLTFVQ
TH_4380|M.thermoresistible__bu KAFHNTCAHRG-----MKIVCGTGVEAEGELRCRFHGWRYSLDGRSSFVP
:. ** : * :* * * *
Mb3186c|M.bovis_AF2122/97 HMAANLD-FNRRECRLPQFAVATWNGLVWINLDADAEP-IAAHLDLTDDE
Rv3161c|M.tuberculosis_H37Rv HMAANLD-FNRRECRLPQFAVATWNGLVWINLDADAEP-IAAHLDLTDDE
MMAR_0230|M.marinum_M HAEAFPD-LDMSARGLIEVESHEVDGLIVIGPLDPVDAGLIEDEGAIGWL
MUL_4884|M.ulcerans_Agy99 HAEAFPD-LDMSARGLVEVESHEVDGLIVIGPLDPVDAGLIEDEGAIGWL
MAV_1644|M.avium_104 GREGFPD-VTLRSAGLTELPATEFAGFLWVSLDPGATLDVATHLGPLADE
Mvan_4910|M.vanbaalenii_PYR-1 GKAGFDS-MDKADYGLVELPNQERHGFIWAVLTAGATIDVDAHLGAIGPE
MSMEG_0657|M.smegmatis_MC2_155 GFKDVDG-FDPGGFGLVELRLVDWHGWLFVDP-SGQDADFATHVAGLEEV
MAB_4370c|M.abscessus_ATCC_199 NEEGYPDSFSTDDWGLVEVPHVDSYHGLIFGTWNPAPVGLRESLGDMAWY
Mflv_2464|M.gilvum_PYR-GCK QEGEFFD-LDKSRYGLVPVHCEVWEGFIFVNFAPEPEQSLREFLGPMITN
TH_4380|M.thermoresistible__bu GREEFAN-RPREQWGLRPVHVGTWGGWVFISMADEPPE-LLEWLDPLPTA
. * . : .
Mb3186c|M.bovis_AF2122/97 FAGYRLGE----MVQVESWSHEWR-----ANWKVAAENGHEN-YHVLGLH
Rv3161c|M.tuberculosis_H37Rv FAGYRLGE----MVQVESWSHEWR-----ANWKVAAENGHEN-YHVLGLH
MMAR_0230|M.marinum_M TDGSPWRA----KLVPAERLIVVESTLREINWKVLVEQFLEG-YHIRSTH
MUL_4884|M.ulcerans_Agy99 TDGSPWRA----KLVPAERLIVVESTLREINWKVLVEQFLEG-YHIRSTH
MAV_1644|M.avium_104 LDSWGIGR----WSPLGEKVLDCA-----INWKLAIDTFAEN-YHFATVH
Mvan_4910|M.vanbaalenii_PYR-1 LELMNYAS----YGYHTEKEFQSQ-----VSWKGALEAFAEG-YHFPFVH
MSMEG_0657|M.smegmatis_MC2_155 VSPYRPED----LTIVARHSYELA-----ANWKIIAENYQEC-YHCSSIH
MAB_4370c|M.abscessus_ATCC_199 MDAMLDHDSEGTVVVGGVHKWVLDG-----NWKLAAEQFATDWYHVNMSH
Mflv_2464|M.gilvum_PYR-GCK LEGYPFDR----MTSRFHYRSEVK-----ANWKLYMDAFQEF-YHAPVLH
TH_4380|M.thermoresistible__bu LAPFRLQD----MRFRWRKRTTLP-----ANYKTVIDAFIEG-YHTPGTH
.:* : ** *
Mb3186c|M.bovis_AF2122/97 ---RQTLEPFVPGGGDLDVRQY------------SRWALRLRVPFTVPVE
Rv3161c|M.tuberculosis_H37Rv ---RQTLEPFVPGGGDLDVRQY------------SRWALRLRVPFTVPVE
MMAR_0230|M.marinum_M ---KDTFFPVQYDDLNVVEAFG------------PNTRITFPYRNIERLR
MUL_4884|M.ulcerans_Agy99 ---KDTFFPVQYDDLNVVEAFG------------PNTRITFPYRNIERLR
MAV_1644|M.avium_104 ---RQTFATIARSNCTVFDSYG------------PHHRLVFPLNTILELD
Mvan_4910|M.vanbaalenii_PYR-1 G--ESVIGQNTLPNTGVYDEYG------------KHHRIGFPFNWVTNLA
MSMEG_0657|M.smegmatis_MC2_155 PELSRISPPTSGENLELPGAWM------------GGWMSIIDGAETMSLD
MAB_4370c|M.abscessus_ATCC_199 ASALMVMAPQGKAPKDEIVHRTGRQYVD-----PNGHGAGFPVHPKNRFD
Mflv_2464|M.gilvum_PYR-GCK ANQSPTAYSKAAAQAGFEAPHYQIDG--------PHRLVSTSGVRAWEMA
TH_4380|M.thermoresistible__bu PQTLRTAQSPHPSTEPAKPQEYVYAPYTPTIAYKNHSRFIYTQRPETAER
Mb3186c|M.bovis_AF2122/97 AKS----------------------------------LQLNEVQKSNLVV
Rv3161c|M.tuberculosis_H37Rv AKS----------------------------------LQLNEVQKSNLVV
MMAR_0230|M.marinum_M DRP----------------------------------EHTWTTGARVTYL
MUL_4884|M.ulcerans_Agy99 DRP----------------------------------EHTWTTGARVTYL
MAV_1644|M.avium_104 DIP----------------------------------EDQWNPFHNMVVI
Mvan_4910|M.vanbaalenii_PYR-1 DDA----------------------------------SGSWDPHQNMGVI
MSMEG_0657|M.smegmatis_MC2_155 GRSGGVA-----------------------------IAGLSEHELRTVMY
MAB_4370c|M.abscessus_ATCC_199 ARVVHQWTDYGAL--------------------RERLGEARVQGPLTNGH
Mflv_2464|M.gilvum_PYR-GCK DEMRKPIEDICQSGL-------FGPWDKPDLGEMPAGLNPAKCDPWGLDS
TH_4380|M.thermoresistible__bu DKGRQEQASRPEVFANAMQYNYIGVNALVTERDYRAAQKLATMEPSEVPP
Mb3186c|M.bovis_AF2122/97 LWTFPNS---------------------------------ALAIAGERVV
Rv3161c|M.tuberculosis_H37Rv LWTFPNS---------------------------------ALAIAGERVV
MMAR_0230|M.marinum_M YQLFPNV---------------------------------MLATFPDVIA
MUL_4884|M.ulcerans_Agy99 YQLFPNV---------------------------------MLATFPDVIA
MAV_1644|M.avium_104 YALFPNI---------------------------------VLSVTIANGE
Mvan_4910|M.vanbaalenii_PYR-1 YWIYPNL---------------------------------ILANSPIGVE
MSMEG_0657|M.smegmatis_MC2_155 LVGYPNL---------------------------------LVSLHPDYVM
MAB_4370c|M.abscessus_ATCC_199 ATVFPNLS--------------------------------YLPVNGSVRI
Mflv_2464|M.gilvum_PYR-GCK FQLFPNFV--------------------------------ILFWGQGWYL
TH_4380|M.thermoresistible__bu FVLYHQLCEELALAEGVDFPRMSMEEYFAGNGDWHIFPTMVILVEKSCLL
: : :
Mb3186c|M.bovis_AF2122/97 WFGFIPQSIDRVQVLGGVLTTPELAADAAATAQT-------SQFVMAMIN
Rv3161c|M.tuberculosis_H37Rv WFGFIPQSIDRVQVLGGVLTTPELAADAAATAQT-------SQFVMAMIN
MMAR_0230|M.marinum_M MVVIDPVSVDRTAVTIYSMVTPDVAERDSLDASSNLA---GAGSFIGQGL
MUL_4884|M.ulcerans_Agy99 MVVIDPVSVDRTAVTIYSMVTPDVAERDSLDASSNLA---GAGSFIGQGL
MAV_1644|M.avium_104 LFRVYPGDRPGRSITVHQNATPQDLSDESVAAGVQAV---FD-YAHATVR
Mvan_4910|M.vanbaalenii_PYR-1 IIDILPAGEPTRCSVRHSWMGRVPATTDEMREMYDQV---YD-SVHAAVV
MSMEG_0657|M.smegmatis_MC2_155 THLMTPLAVDRTHVECAWAFPREAAERPGFDPAY-------AVDFWDLTN
MAB_4370c|M.abscessus_ATCC_199 WHPKGPDKMEVWAWTIVDRSMPEEVRDAQRLYNLRTF---GPTGIFEQDD
Mflv_2464|M.gilvum_PYR-GCK TYHYWPTSHNTHIFEGTVYFPQPRTPRERIAQELAAVS-FKEYGLQDANT
TH_4380|M.thermoresistible__bu GYRMLPDAEDPDKCTFEMFTLEHFAPGEVPETSWQVFERWQDHDGWGELP
*
Mb3186c|M.bovis_AF2122/97 DEDRLGLEAVQVGAGSRFAERGHLSSKEWPGMLAFYRNLAMALVGDHPGA
Rv3161c|M.tuberculosis_H37Rv DEDRLGLEAVQVGAGSRFAERGHLSSKEWPGMLAFYRNLAMALVGDHPGA
MMAR_0230|M.marinum_M SEDNEMSEGVQRGLAAGANAFVEFGRHES-AVGRFHAALGRRLARLTSVG
MUL_4884|M.ulcerans_Agy99 FEDNEMSEGVQRGLAAGANAFVEFGRHES-AVGRFHAALGRRLARLTSAG
MAV_1644|M.avium_104 DEDYRLVESLQANLESGARDHLVFGRNEP-GLQHRHITWAKALAASTG--
Mvan_4910|M.vanbaalenii_PYR-1 DEDFAMLPQCGEGVRHGQHDHMIIGRNEI-GVQHMVKVFAQELGVALS--
MSMEG_0657|M.smegmatis_MC2_155 RQDWAACESVQRGLSSPHARPGPLAPDED-GVYQFVTRVARAYQGA----
MAB_4370c|M.abscessus_ATCC_199 GENWSEVQSISRGFVTNSVALNYQMGLDSEREDGRHPGLTSELYSDAAGR
Mflv_2464|M.gilvum_PYR-GCK LEATQSMVESRVLDDFVLCDQEVLIRHLHTETAAWVDDYRRKTQQVKEGV
TH_4380|M.thermoresistible__bu TQDLQNIEAIHAGMHSHGFEGLWLNTAQEMSIRNEHAIADRFIFGLDKQ-
:
Mb3186c|M.bovis_AF2122/97 S-------------------
Rv3161c|M.tuberculosis_H37Rv S-------------------
MMAR_0230|M.marinum_M --------------------
MUL_4884|M.ulcerans_Agy99 --------------------
MAV_1644|M.avium_104 --------------------
Mvan_4910|M.vanbaalenii_PYR-1 --------------------
MSMEG_0657|M.smegmatis_MC2_155 --------------------
MAB_4370c|M.abscessus_ATCC_199 SFYARWRDLMNTKAWHEEHV
Mflv_2464|M.gilvum_PYR-GCK --------------------
TH_4380|M.thermoresistible__bu --------------------