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VARFPKPPEGSWTEHYPQLGTGPVSYEDSIDPEFYELERKAVFRRAWLNVGRVEQVPRKGSYFTKEMKVV NTSIIVVRTTSGEVKAYHNICRHRGNKLVWNDMPLEETSGVCRQFTCKYHAWRYDLDGNLTFVQQEGEFF DLDKSRYGLVPVHCEVWEGFIFVNFAPEPEQSLREFLGPMITNLEGYPFDRMTSRFHYRSEVKANWKLYM DAFQEFYHAPVLHANQSPTAYSKAAAQAGFEAPHYQIDGPHRLVSTSGVRAWEMADEMRKPIEDICQSGL FGPWDKPDLGEMPAGLNPAKCDPWGLDSFQLFPNFVILFWGQGWYLTYHYWPTSHNTHIFEGTVYFPQPR TPRERIAQELAAVSFKEYGLQDANTLEATQSMVESRVLDDFVLCDQEVLIRHLHTETAAWVDDYRRKTQQ VKEGV
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. gilvum PYR-GCK | Mflv_2464 | - | - | 100% (425) | Rieske (2Fe-2S) domain-containing protein |
| M. gilvum PYR-GCK | Mflv_2470 | - | e-152 | 58.75% (417) | Rieske (2Fe-2S) domain-containing protein |
| M. gilvum PYR-GCK | Mflv_2439 | - | e-149 | 56.74% (430) | Rieske (2Fe-2S) domain-containing protein |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb3186c | - | 2e-22 | 30.43% (207) | dioxygenase |
| M. tuberculosis H37Rv | Rv3161c | - | 8e-23 | 30.92% (207) | dioxygenase |
| M. leprae Br4923 | - | - | - | - | - |
| M. abscessus ATCC 19977 | MAB_4370c | - | 7e-20 | 33.17% (202) | putative large terminal subunit of phenylpropionate dioxygenase |
| M. marinum M | MMAR_4744 | - | 1e-154 | 59.33% (418) | dioxygenase |
| M. avium 104 | MAV_3967 | - | 0.0 | 87.02% (416) | Rieske (2Fe-2S) domain-containing protein |
| M. smegmatis MC2 155 | MSMEG_0299 | - | 0.0 | 85.51% (421) | Rieske (2Fe-2S) domain-containing protein |
| M. thermoresistible (build 8) | TH_3362 | - | 0.0 | 87.06% (425) | PUTATIVE Rieske (2Fe-2S) domain protein |
| M. ulcerans Agy99 | MUL_3820 | - | 6e-16 | 25.37% (201) | ring-hydroxylating dioxygenase, large terminal subunit |
| M. vanbaalenii PYR-1 | Mvan_4190 | - | 0.0 | 93.82% (421) | Rieske (2Fe-2S) domain-containing protein |
CLUSTAL 2.0.9 multiple sequence alignment
Mb3186c|M.bovis_AF2122/97 --MLSTDNRAELGDILTDIGDYLDDNPPALSLPP---AAYTS---SELWQ
Rv3161c|M.tuberculosis_H37Rv --MLSTDNRAELGDILTDIGDYLDDNPPALSLPP---AAYTS---SELWQ
Mflv_2464|M.gilvum_PYR-GCK -----------MARFPKPPEGSWTEHYPQLGTGP---VSYEDSIDPEFYE
Mvan_4190|M.vanbaalenii_PYR-1 -----------MARFPKPPEGSWTQHYPQLGTGP---VSYRDSVDPAFYE
MAV_3967|M.avium_104 -----------MARFPKPPEGSWTQHYPELGTAP---VSYEDSISPEVYE
TH_3362|M.thermoresistible__bu -----------MARFPKPPEGSWTEHHPELGTAP---VSYRDCTDPEIYQ
MSMEG_0299|M.smegmatis_MC2_155 -----------MARFPKPAEGSWTQHYPELGTGP---VSYEDSISPEIYE
MMAR_4744|M.marinum_M -----------MPHFTKPAAGSWTQNYPELGTAP---VDYTDSIDPQYFA
MUL_3820|M.ulcerans_Agy99 ------MDRDQLIDLTRRALKLARDKTTDLAPHQ---HAVDAREYTSLDR
MAB_4370c|M.abscessus_ATCC_199 MASLPRPVAVAQGALCRPQALVRDIDVSGLVDSHGGLLDRVIFTDGELYR
: . . *
Mb3186c|M.bovis_AF2122/97 LER-ERIFNRSWMLVAHVDQLAKTGDYVTVSVAGEPV----MVVRDVDGQ
Rv3161c|M.tuberculosis_H37Rv LER-ERIFNRSWMLVAHVDQVAKTGDYVTVSVAGEPV----MVVRDVDGQ
Mflv_2464|M.gilvum_PYR-GCK LER-KAVFRRAWLNVGRVEQVPRKGSYFTKEMKVVNT--SIIVVRTTSGE
Mvan_4190|M.vanbaalenii_PYR-1 LER-KAVFRRAWLNVGRVEQIPRKGSYFTKEMKVVNT--SIIVVRTTTGE
MAV_3967|M.avium_104 LER-EAIFKRAWLNVARVEQLPRKGSYLTRELKVVNT--SIILVRNGSGE
TH_3362|M.thermoresistible__bu KER-HAIFKRAWLNVGRVERLPRKGSYFTKEIKVVNT--SIIVVRTGSGE
MSMEG_0299|M.smegmatis_MC2_155 LER-QAIFKRAWLNVGRVELLPRKGSYFTKELKAANT--SIIVVRNKSGK
MMAR_4744|M.marinum_M DEQ-RAIFRRTWLNVGRIERLPRTGSYFTKELPSTGAGMSVIVVKGKDGV
MUL_3820|M.ulcerans_Agy99 HERDRAMVLDSPQLVGYSSELPGPDTYRTKTIMGRSI----LLTRAADRA
MAB_4370c|M.abscessus_ATCC_199 QEL-RRVFAPSWLFLAHADQFHRPGDFFTTYMGEDPV----IVSMDKNRR
* . :. : :. . . . : * : ::
Mb3186c|M.bovis_AF2122/97 LHALSPICRHR------LMLMVEPGAGRIDTLTCQYHLWRYGLDGRLRGA
Rv3161c|M.tuberculosis_H37Rv LHALSPICRHR------LMLMVEPGAGRIDTLTCQYHLWRYGLDGRLRGA
Mflv_2464|M.gilvum_PYR-GCK VKAYHNICRHRGNKLVWNDMPLEETSGVCRQFTCKYHAWRYDLDGNLTFV
Mvan_4190|M.vanbaalenii_PYR-1 VKAYHNICRHRGNKLVWNDMPLEETSGVCRQFTCKYHAWRYDLDGNLTFV
MAV_3967|M.avium_104 IKAFHNVCRHRGNKLVWNDMPLEETRGVCRQFTCKYHAWRYDLDGNLTFV
TH_3362|M.thermoresistible__bu IKAFHNICRHRGNKLVWNDMPLEETSGVCRQFTCKYHAWRYDLDGNLTFV
MSMEG_0299|M.smegmatis_MC2_155 INAFHNVCRHRGNKLVWDDLPQQETSGVCRQFTCKYHAWRYDLDGDLTFV
MMAR_4744|M.marinum_M IRAFHNICRHRGNKLVWNDFPHEETAGTCRQFTCKYHAWRYGLDGELTFI
MUL_3820|M.ulcerans_Agy99 VRAFDNVCLHR-------QSRVATGCGTALRFTCPYHGWTYDNGGRLVGL
MAB_4370c|M.abscessus_ATCC_199 IRAFLNSCRHRG------ARVCRADYGVTRGFTCTYHGWAYDLEGKLISV
:.* * ** * :** ** * *. * *
Mb3186c|M.bovis_AF2122/97 PHMAANLD-FNRRECRLPQF-AVATWNGLVWINLDAD---AEP-IAAHLD
Rv3161c|M.tuberculosis_H37Rv PHMAANLD-FNRRECRLPQF-AVATWNGLVWINLDAD---AEP-IAAHLD
Mflv_2464|M.gilvum_PYR-GCK QQEGEFFD-LDKSRYGLVPV-HCEVWEGFIFVNFAPE---PEQSLREFLG
Mvan_4190|M.vanbaalenii_PYR-1 QQEGEFFD-LDKSRYGLVPV-HCDVWEGFIFVNFAKQ---PEQSLRDFLG
MAV_3967|M.avium_104 QQEEEFFD-LDKSRYGLVPV-HCDVWEGFVFVNFAKT---PEQSLREFLG
TH_3362|M.thermoresistible__bu QQEEEFFD-LHKSRYGLVPV-HCDVWEGFIFVNFAEQ---PEQSLREFLG
MSMEG_0299|M.smegmatis_MC2_155 QQEEEFFD-LDKSRYGLVPV-HCQVWEGFIFVNFAQE---PEQSLRDFLG
MMAR_4744|M.marinum_M QQEDEFFD-VDKSQYGLKSV-RCEVWEGFIFINLDP----AAAPLSEYLG
MUL_3820|M.ulcerans_Agy99 PGREGFPG-IAVGSDGLTEL-PATEFAGFLWVSLHPA---ARLDVPAHLG
MAB_4370c|M.abscessus_ATCC_199 PNEEGYPDSFSTDDWGLVEVPHVDSYHGLIFGTWNPAPVGLRESLGDMAW
. . * . : *::: . :
Mb3186c|M.bovis_AF2122/97 LTDDEFAGYRLGEMVQVESWSHEWRANWKVAAENGHENYHVLGLHRQT--
Rv3161c|M.tuberculosis_H37Rv LTDDEFAGYRLGEMVQVESWSHEWRANWKVAAENGHENYHVLGLHRQT--
Mflv_2464|M.gilvum_PYR-GCK PMITNLEGYPFDRMTSRFHYRSEVKANWKLYMDAFQEFYHAPVLHANQSP
Mvan_4190|M.vanbaalenii_PYR-1 PMITDLEGYPFDRMTSRFHYRSEVKANWKLYMDAFQEFYHAPVLHANQSP
MAV_3967|M.avium_104 PMITALEGYPFDKMTSRWCYRSEVKANWKLYMDAFQEFYHAPVLHANQSP
TH_3362|M.thermoresistible__bu PMITGLEGYPFDRMTSRFTYRSEVKANWKLYMDAFQEFYHAPVLHANQSP
MSMEG_0299|M.smegmatis_MC2_155 PMITNLEGYPFDKLTSRFTYRSEVKANWKLYMDAFQEFYHAPVLHANQSP
MMAR_4744|M.marinum_M SMAKGLQGYPFHEMTEVYSYRAEVGSNWKLFIDAFAEFYHAPVLHQGQYT
MUL_3820|M.ulcerans_Agy99 PLAGELQSWGIGRWSPLGEKVLGSPIDWKLATDTFAENYHFATVHRRT--
MAB_4370c|M.abscessus_ATCC_199 YMDAMLDHDSEGTVVVGGVHKWVLDGNWKLAAEQFATDWYHVNMSHASAL
: :**: : :: :
Mb3186c|M.bovis_AF2122/97 -------------LEPFVPGGGDLDVRQYSRWALRLRVPFTVPVEAKS--
Rv3161c|M.tuberculosis_H37Rv -------------LEPFVPGGGDLDVRQYSRWALRLRVPFTVPVEAKS--
Mflv_2464|M.gilvum_PYR-GCK TAYSKAAAQAGFEAPHYQIDGPHRLVSTSGVRAWEMADEMRKPIEDICQS
Mvan_4190|M.vanbaalenii_PYR-1 TAYSKAAAEAGFEAPHYRIEGPHRLVSTSGIRAWELADEMRKPIEDICES
MAV_3967|M.avium_104 TAYSKAAAEAGFEAPHYRLDGPHRLVSTSGVRAWEMSPEMRKPIEDICRS
TH_3362|M.thermoresistible__bu TAYSKAAAEAGFEAPHYRIDGPHRLVSTSGVRAWEMSEEMRKPIEDICRS
MSMEG_0299|M.smegmatis_MC2_155 TAYSRAAAEAGFEAPHYRIEGPHRLVSTSGVRAWEMPDEMRKPIEDICQS
MMAR_4744|M.marinum_M KEEAAKIMKYGFEALHYELAGPHAMISVWGGQSPPADLKMVKPMDRVLRS
MUL_3820|M.ulcerans_Agy99 ---------------FATIARSNCTVFDSYGPHHRLIFPLNTILELEN--
MAB_4370c|M.abscessus_ATCC_199 MVMAPQGKAP--KDEIVHRTGRQYVDPNGHGAGFPVHPKNRFDARVVHQW
.
Mb3186c|M.bovis_AF2122/97 ---------------------LQLNEVQ-KSN--LVVLWTFPNSALAIAG
Rv3161c|M.tuberculosis_H37Rv ---------------------LQLNEVQ-KSN--LVVLWTFPNSALAIAG
Mflv_2464|M.gilvum_PYR-GCK GLFGPWDKP----DLGEMPAGLNPAKCD-PWG--LDSFQLFPNFVILFWG
Mvan_4190|M.vanbaalenii_PYR-1 GLFGPWDKP----DLGEMPVGLNPAKCD-PWG--LDSFQLFPNFVMLFWG
MAV_3967|M.avium_104 GLFGPWDKP----DLGPMPDGLNPAKCD-PWG--LDSFQLFPNFVILFWG
TH_3362|M.thermoresistible__bu GLFGPWDKP----DLGEMPKGLNPARCD-PWG--LDSFQLFPNFVILFWG
MSMEG_0299|M.smegmatis_MC2_155 GLFGPWDKP----DLGEMPVGLNPAKCD-PWG--LDSFQLFPNFVMLFWG
MMAR_4744|M.marinum_M GLFGPWDRPAPITDLEELPPGINPTKAK-QWG--IDSFLFFPNFMLLIWE
MUL_3820|M.ulcerans_Agy99 ---------------------VPEGQWD-PFHHMVVIYALFPNVVLSVTI
MAB_4370c|M.abscessus_ATCC_199 TDYGALRER------------LGEARVQGPLTN--GHATVFPNLSYLPVN
: . . ***
Mb3186c|M.bovis_AF2122/97 -ERVVWFGFIPQSIDRVQVLGGVLTTPELAADAAATAQTSQFVMAMINDE
Rv3161c|M.tuberculosis_H37Rv -ERVVWFGFIPQSIDRVQVLGGVLTTPELAADAAATAQTSQFVMAMINDE
Mflv_2464|M.gilvum_PYR-GCK QGWYLTYHYWPTSHNTHIFEGTVYFPQPRTPRERIAQELAAVSFKEYGLQ
Mvan_4190|M.vanbaalenii_PYR-1 QGWYLTYHYWPTSHNTHLFEGTVYFPQPRTPRERIAQELAAVSFKEYGLQ
MAV_3967|M.avium_104 QGWYLTYHYWPTSYHSHVFEGTLYFPAPRTPRERVAQELAAVSFKEYGLQ
TH_3362|M.thermoresistible__bu QGWYLTYHYWPTSYNTHVFEGNLYFPAARTPRERVAQELAAASFKEYGLQ
MSMEG_0299|M.smegmatis_MC2_155 QGWYLTYHYWPTSYNTHTFECTLYFPQARTPRERLAQELAAASFKEYGLQ
MMAR_4744|M.marinum_M PGWYLTYHYWPTTVDKHIFETTLYFVPPRNARERLAQELAAVTFKEYALQ
MUL_3820|M.ulcerans_Agy99 ANGELFRIYPGDRPGSSITVHQNSTPLDLSDESVATGAQAVFDYAHATVR
MAB_4370c|M.abscessus_ATCC_199 GSVRIWHPKGPDKMEVWAWTIVDRSMPEEVRDAQRLYNLRTFGPTGIFEQ
: .
Mb3186c|M.bovis_AF2122/97 DR--LGLEAVQVGAGSRFAERGHLSSKEWPGMLAFYRNLAMALVGDHPGA
Rv3161c|M.tuberculosis_H37Rv DR--LGLEAVQVGAGSRFAERGHLSSKEWPGMLAFYRNLAMALVGDHPGA
Mflv_2464|M.gilvum_PYR-GCK DA--NTLEATQSMVESRVLDDFVLCDQ--EVLIRHLHTETAAWVDDYRRK
Mvan_4190|M.vanbaalenii_PYR-1 DA--NTLEATQSMVESEVLDEFVLCDQ--EVLIRHLHTETAAWVDEYRRK
MAV_3967|M.avium_104 DA--NTLEATQTMLESRVVDKFVLNDQ--EILLRHLHKETAAWIDDYRRM
TH_3362|M.thermoresistible__bu DA--NTLEATQSMIESGVLDDFVLCDQ--EVLIRHLHKETADWIDRYERE
MSMEG_0299|M.smegmatis_MC2_155 DA--NTLEATQSMIETRVVEEFLLCDQ--EILLRHLHSETAAWIEDYQRK
MMAR_4744|M.marinum_M DS--NTLEATQTMIGTGVVKDFPLCDQ--EILLRHLHKMTGDYVKEYRNN
MUL_3820|M.ulcerans_Agy99 DEDYRLAETLQTNLESGARQRLVFGRN-----EPGLQHRHITWAQALETP
MAB_4370c|M.abscessus_ATCC_199 DDG-ENWSEVQSISRGFVTNSVALNYQMGLDSEREDGRHPGLTSELYSDA
* . * . : :
Mb3186c|M.bovis_AF2122/97 S----------------------
Rv3161c|M.tuberculosis_H37Rv S----------------------
Mflv_2464|M.gilvum_PYR-GCK TQQVKEGV---------------
Mvan_4190|M.vanbaalenii_PYR-1 TAGV-------------------
MAV_3967|M.avium_104 ST---AGV---------------
TH_3362|M.thermoresistible__bu STGTAAGVAGR------------
MSMEG_0299|M.smegmatis_MC2_155 TAGV-------------------
MMAR_4744|M.marinum_M GHAK-------------------
MUL_3820|M.ulcerans_Agy99 G----------------------
MAB_4370c|M.abscessus_ATCC_199 AGRSFYARWRDLMNTKAWHEEHV