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PDNDTVTEGFTERFAAALDGYGERPCIEYDGRWYSGDEVMSYGAQIASVLHSAGVTGDAPVGLIVRNRLQ HAAAIAGFLAAGRTVAMIYSFQSPDAIARDIEQLNLAAVIADPEDWTEPVVTGAARVGSAGVAISLSAPT VAGVVGLERCDTARRHAPSEPGVALKILTSGTTGPPKRQSIRFNVLERTVFSVTAGEKASGDAPPEFAYW QFGGIGVCQLIAGLYNGRRIAMLERFTVDGWVDAVRRHRVTRCGVQPAVIRMLLDADVPKEDLASLEFLI SASGPLDPETRDEFEQQYGIPIRLAYGATEFAGSLCAWTPELLQQYGESKRNSVGRALPDTELRVVDPET GGERAVGEQGLLEAKVAPIGPDWIRTTDIASIDEDGFVTLHGRADGAINRGGFKVLPEAVRRVLITHPDV RDACVVGVPDARLGQVPFAAIEVSAGTAAPSDGELRELVRQSLPVYNVPVAFAAVEELPRNPALKVSLPA VAALYSGHTGD*
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. vanbaalenii PYR-1 | Mvan_5064 | - | - | 100% (502) | AMP-dependent synthetase and ligase |
| M. vanbaalenii PYR-1 | Mvan_1982 | - | 5e-76 | 36.51% (482) | AMP-dependent synthetase and ligase |
| M. vanbaalenii PYR-1 | Mvan_0943 | - | 5e-36 | 31.28% (374) | AMP-dependent synthetase and ligase |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb1462c | fadD12 | 5e-28 | 26.59% (504) | acyl-CoA synthetase |
| M. gilvum PYR-GCK | Mflv_1694 | - | 0.0 | 80.85% (496) | AMP-dependent synthetase and ligase |
| M. tuberculosis H37Rv | Rv1427c | fadD12 | 5e-28 | 26.59% (504) | acyl-CoA synthetase |
| M. leprae Br4923 | MLBr_02546 | fadD2 | 5e-22 | 24.42% (471) | acyl-CoA synthetase |
| M. abscessus ATCC 19977 | MAB_2539c | - | 6e-29 | 30.41% (365) | fatty-acid-CoA ligase FadD |
| M. marinum M | MMAR_4025 | - | 2e-71 | 36.73% (490) | long-chain fatty-acid CoA ligase |
| M. avium 104 | MAV_2596 | - | 3e-78 | 38.64% (484) | AMP-dependent synthetase and ligase |
| M. smegmatis MC2 155 | MSMEG_2217 | - | 2e-75 | 37.35% (498) | AMP-dependent synthetase and ligase |
| M. thermoresistible (build 8) | TH_3166 | - | 1e-87 | 39.22% (487) | PUTATIVE putative AMP-dependent synthetase and ligase |
| M. ulcerans Agy99 | MUL_3890 | - | 4e-70 | 36.73% (490) | long-chain fatty-acid CoA ligase |
CLUSTAL 2.0.9 multiple sequence alignment
Mvan_5064|M.vanbaalenii_PYR-1 ---------------------------------------MPDNDTVTEGF
Mflv_1694|M.gilvum_PYR-GCK ---------------------------------------MPHNGGVTEGL
MMAR_4025|M.marinum_M --------------------------------------------MSNHPL
MUL_3890|M.ulcerans_Agy99 --------------------------------------------MSNHPL
MSMEG_2217|M.smegmatis_MC2_155 ---------------------------------------------MAHPL
TH_3166|M.thermoresistible__bu --------------------------------------------------
MAV_2596|M.avium_104 ----------------------------------------------MTSI
Mb1462c|M.bovis_AF2122/97 --MRIRQAFG-------LIATMRRAGLIAPLRPDRYLRIVAAMRREGMGF
Rv1427c|M.tuberculosis_H37Rv --MRIRQAFG-------LIATMRRAGLIAPLRPDRYLRIVAAMRREGMGF
MLBr_02546|M.leprae_Br4923 -MTKLRHYLERGAAEVHYLRSIFESGALGFEPPLTYAAMAVAMRKWG-DL
MAB_2539c|M.abscessus_ATCC_199 MPNNLRESLRQWG---AAIPAAVTSGAFTPVSPRALASLVRSFWQFGPTP
Mvan_5064|M.vanbaalenii_PYR-1 TERFAAALDGYGERPCIEYDGRWYSGDEVMSYGAQIASVLHSAGVTGDAP
Mflv_1694|M.gilvum_PYR-GCK TESFAAALHSYADRPCIEFDGRWYTGDEVVAYGAAIASLLETAGVPGDAP
MMAR_4025|M.marinum_M SQRIADTLDLQPDARAVEYDRAWLSWGQLGAATRRVAA---LAARQPSPE
MUL_3890|M.ulcerans_Agy99 SQRIAVTLDLQPDARAVEYDRAWLSLGQLGAATRRVAA---LAAKQPSPE
MSMEG_2217|M.smegmatis_MC2_155 SQRIADVLDLDPDAGAIQFDGQWSCWGQIAALAGHIEK---ITG---PAR
TH_3166|M.thermoresistible__bu ------VLALDPSAEAVEYQGAWTDWKTVAHVAHAVESRLTAAGLGAGSP
MAV_2596|M.avium_104 DDALGRLWDADDGDRMLECDGRWASWGSVRSLTERIDRELTAAGCQTGGR
Mb1462c|M.bovis_AF2122/97 TAGFAGAARRCPDRPGLIDELGTLTWRQLDERGNALAAALQALPAGPPRV
Rv1427c|M.tuberculosis_H37Rv TAGFAGAARRCPDRPGLIDELGTLTWRQLDERGNALAAALQALPAGPPRV
MLBr_02546|M.leprae_Br4923 AMLPAMPARRSPHRAALIDEEGTLTYKELDRAAHALANGLIAKGVRGGDG
MAB_2539c|M.abscessus_ATCC_199 AMLLAASAIRFPDRAALIDDSGQLTYRDLQRRAEAVAAAVYARTPSAPRS
: : : :
Mvan_5064|M.vanbaalenii_PYR-1 VGLIVRNRLQHAAAIAGFLAAGRTVAMIYSFQSPDAIARDIEQLNLAAVI
Mflv_1694|M.gilvum_PYR-GCK VGLIVRNRLPHAAAIAGFLAAGRTVAMIYSFQSAGAIAADVERLNLAAVV
MMAR_4025|M.marinum_M IGILLRSRPAQMAAFLGVLLSGATVVTINPSRGDERTRADIAALELPLIA
MUL_3890|M.ulcerans_Agy99 IGILLRNRPAQMAAFLGVLLAGATVVTINPSRGDERTRADIAALELPLIA
MSMEG_2217|M.smegmatis_MC2_155 VGIMLRNHPVHVAALLGVLAAGGTVVVINPARGDDRTRADLEALALPVVV
TH_3166|M.thermoresistible__bu VGLILRNHPAMVAALLGVLLVNGCVVTINASSGDARLATDLEELRLPVVI
MAV_2596|M.avium_104 VAVVLSNRMESVAALIAIFRGGRTLVTISPLQPPDRLSDDLGASAAPFVL
Mb1462c|M.bovis_AF2122/97 VGIMCRNHRGFVDALLAVNRIGAHILLLNTSFAGPALAEVVTREGVDTVV
Rv1427c|M.tuberculosis_H37Rv VGIMCRNHRGFVDALLAVNRIGAHILLLNTSFAGPALAEVVTREGVDTVV
MLBr_02546|M.leprae_Br4923 IAILARNHRWFVIANYGCARVGARIILLNSEFSGPQIKEVSEREGAKVII
MAB_2539c|M.abscessus_ATCC_199 VGIICRNHRGFAESMLAGAQLGAELVFINTELTPQQLGAILQRHEPDVLV
:.:: .: : . . : : . :
Mvan_5064|M.vanbaalenii_PYR-1 ADPEDWTEPVVTGAARVGSAGVAISLSAPT-----VAGVVGLERCDTA--
Mflv_1694|M.gilvum_PYR-GCK ADAEDWTEPVVAAAARIGAAGVAISLGTPP-----VVAVPRLEHRDSS--
MMAR_4025|M.marinum_M GSDEDLG---LLVTNTTDSATVSLSDLF---------DHPGEPGDHT---
MUL_3890|M.ulcerans_Agy99 GSDEDLG---LLVTNTTDSATVSLSDLF---------DHPGEPGGHT---
MSMEG_2217|M.smegmatis_MC2_155 GLAEDLAH--LMPAHSPRTTRVAIAGLD---------TEPVVTAAESP--
TH_3166|M.thermoresistible__bu GLGADWGRPHIVEAAAGALGLEVAADPA---------SVRTVTGRGSVGA
MAV_2596|M.avium_104 APAALWSD-EVFNRVVADLGATGWRLDD---------GELDLQVRGTT--
Mb1462c|M.bovis_AF2122/97 YDEEFSATVDRALAEKPQATRIVAWTDEDH-----DLTVEKLVAAHAG--
Rv1427c|M.tuberculosis_H37Rv YDEEFSATVDRALAEKPQATRIVAWTDEDH-----DLTVEKLVAAHAG--
MLBr_02546|M.leprae_Br4923 YDDEYTKVVSKAEPALGKLRALGVNPDEEEPPGSTDETLAELIARSSS--
MAB_2539c|M.abscessus_ATCC_199 YDDEYSQAVEESQYQGLRVLAWRENPQENR------LTLDSLADQHHP--
Mvan_5064|M.vanbaalenii_PYR-1 RRHAPSEPGVALKILTSGTTGPPKRQSIRFNVLERTVFSVTAGE----KA
Mflv_1694|M.gilvum_PYR-GCK HAHAPAEPGVALKILTSGTTGPPKRQAIATHVLERTVFSVTAGE----KA
MMAR_4025|M.marinum_M --TRGAGADVAVRMLTSGTTGPPKRVDLSYDMLARSVMGPAPDR---DPA
MUL_3890|M.ulcerans_Agy99 --TRGAGADVAVRMLTSGTTGPPNRVDLSYDMLARSVMGPAPDR---DPA
MSMEG_2217|M.smegmatis_MC2_155 -ATDAGGPGVAVWMLTSGTTGPPKRVDLTYDMLARSVMGREPEK---CPA
TH_3166|M.thermoresistible__bu GPFRPDRPGVAVEMLTSGTTGPPKRVPLSYNAFQHTVAAAGAHYGSGDNG
MAV_2596|M.avium_104 -EAVSGDPAVAIEMLTSGTTGAPKRIPLTRAQLEASLASALRHN---DRP
Mb1462c|M.bovis_AF2122/97 RRPEHTGSHGKVILLTSGTTGTPKGA-RHSGGGIGTLKAI--LDRTPWRA
Rv1427c|M.tuberculosis_H37Rv RRPEHTGSHGKVILLTSGTTGTPKGA-RHSGGGIGTLKAI--LDRTPWRA
MLBr_02546|M.leprae_Br4923 APAPKVRKRASIIILTSGTTGTPKGANRNTPASLAPLGGI--LSKVPFRA
MAB_2539c|M.abscessus_ATCC_199 KPPP-VRRPVKLTLLTSGTTGLAKGVPRAIKPRQLALMCVTAMATVRLRS
: :******* .: .:
Mvan_5064|M.vanbaalenii_PYR-1 SGDAP------PEFAYWQFGGIG-VCQLIAGLYNGRRIAMLERFTVDGWV
Mflv_1694|M.gilvum_PYR-GCK DGDAP------PEFAYWQFGGIG-VCQLIAGLYNGRRVAMLERFSVEGWV
MMAR_4025|M.marinum_M PTEPR----RGVAIVNSPLVHIGGVYRVLQCIAQARPFVLLDRFELNAWA
MUL_3890|M.ulcerans_Agy99 PTEPR----RGVAIVNSPLVHIGGVYRVLQCIAQARPFVLLDRFELNARA
MSMEG_2217|M.smegmatis_MC2_155 PTELR----RGVAIVNSPLVHVGGVFRVLLCIAEARPFVLLPKFELQRWA
TH_3166|M.thermoresistible__bu QVRLR----SGVAIISSPLVHMSGLFRTLLNICEGRRIALLERFRVDDFV
MAV_2596|M.avium_104 EVRTKPPLTGAVGLVTLPIVHIGGLWSLLQSLVAARPIAMLDRFTVPGWH
Mb1462c|M.bovis_AF2122/97 E---------EVTVIVAPMFHAWGFSQLVLASSLACTIVTRRRFDPEATL
Rv1427c|M.tuberculosis_H37Rv E---------EVTVIVAPMFHAWGFSQLVLASSLACTIVTRRRFDPEATL
MLBr_02546|M.leprae_Br4923 H---------EVTLLPAPMFHALGYLHATLAMFLGSTLVLRRRFKPATVL
MAB_2539c|M.abscessus_ATCC_199 R---------DRVLVAPPFFHGFGLAALLGPLALGGTVMCRRRFDAQQAM
. : . . :*
Mvan_5064|M.vanbaalenii_PYR-1 DAVRRHRVTRCGVQPAVIRMLLDADVP---KEDLASLEFLISASGPLDPE
Mflv_1694|M.gilvum_PYR-GCK DAVKRHRVTRAGVQPAVIRMLLDAKVP---AEDLASLEFLISASGPLDPE
MMAR_4025|M.marinum_M AAVRKHRPRTVSLVPAALRTVLHSDLT---RADLASVRTVTCGTAPLAPD
MUL_3890|M.ulcerans_Agy99 AAVRKHRPRTVSLVPAALRTVLHSDPT---RADLASVRTVTCGTAPLAPD
MSMEG_2217|M.smegmatis_MC2_155 AAVREHRPRAVSLVPAALRTVLHSDLT---REDLAGVRVVTSGTAPLSAD
TH_3166|M.thermoresistible__bu DLVVRHQPKAVSLVPSALAMVLDAGVP---PEVFSSVKVVTSGTAPLPVE
MAV_2596|M.avium_104 AVVKQYRPAVAGLPPAAMRSVLDSDIP---REDLASIRAINAGTSPVDPA
Mb1462c|M.bovis_AF2122/97 DLIDRHHATGLVVVPVMFDRIMDLPAEIRNRYDGRSLRFAAASGSRMRPD
Rv1427c|M.tuberculosis_H37Rv DLIDRHHATGLVVVPVMFDRIMDLPAEIRNRYDGRSLRFAAASGSRMRPD
MLBr_02546|M.leprae_Br4923 EDIEKHQATAMVVVPVMLSRILDTLENLETKSDLSSLRVVFVSGSQLGAE
MAB_2539c|M.abscessus_ATCC_199 TDIRRQRVTVLMAVPVMLQRILALADLDRRDARQLSLRMIVTGAAPISSS
: . : * : :: .:. . . :
Mvan_5064|M.vanbaalenii_PYR-1 TRDEFEQQYGIPIRLAYGATEFAGSLCAWTPELLQQYGESKRNSVGRALP
Mflv_1694|M.gilvum_PYR-GCK TRDEFEARYRIPVRLAYGATEFAGSLCAWTPEMLDEFGKTKRESVGRALP
MMAR_4025|M.marinum_M DADAFTEKYGIPVLTSYAATEFGGGVAGWTLADHSRYWHTKRGSVGRANP
MUL_3890|M.ulcerans_Agy99 DADAFTEKYGIPVLTSYAATEFGGGVAGWTLADHSRYWHTKRGSVGRANP
MSMEG_2217|M.smegmatis_MC2_155 DADAFTEKFGIPVLTSYAATEFGGGVAGWTLPDHGKYWKDKRGSVGRANP
TH_3166|M.thermoresistible__bu LQEAFEERYDVAVLPSYGATEFAGGVAGWNLALHREWAKIKRGSVGRPQR
MAV_2596|M.avium_104 LVDAFFERYGIPILVVYGATEFSGAVAGWTVKDFHTRWTEKRGSVGRAFP
Mb1462c|M.bovis_AF2122/97 VVIAFMDQFGDVIYNNYNATEAG-----MIATATPADLRTAPDTAGRPAE
Rv1427c|M.tuberculosis_H37Rv VVIAFMDQFGDVIYNNYNATEAG-----MIATATPADLRTAPDTAGRPAE
MLBr_02546|M.leprae_Br4923 LATRALEELGPVIYNMYGSTEIA-----FATIAGPKDLQINSATVGPVVK
MAB_2539c|M.abscessus_ATCC_199 TVSGVLEAFGPILVNGYGSTEAG-----VVAIASPKDLVASPNTIGRTAL
: * :** . : *
Mvan_5064|M.vanbaalenii_PYR-1 DTELRVVDPETGGERAVGEQGLLEAKVAP-----------------IGPD
Mflv_1694|M.gilvum_PYR-GCK DTELRIVDAETGRERPPGERGLLEAKVGP-----------------LGPD
MMAR_4025|M.marinum_M GTQLRIVD-TEGAPVERASLGLLEVKPAQ---------------LGVSAP
MUL_3890|M.ulcerans_Agy99 GTQLRIVD-TEGAPVERASLGLLEVKPAQ---------------LGVSAP
MSMEG_2217|M.smegmatis_MC2_155 GAQLRVVD-ENGAPLGPDEPGLLEVIPAQ---------------MGPGSP
TH_3166|M.thermoresistible__bu GREVRIVSPEDGAELPPGTQGQIEVR-------------------TRGGE
MAV_2596|M.avium_104 GVRLRVVA-DDGAAVSRGVTGRLQVATLQ---------------AGLAGD
Mb1462c|M.bovis_AF2122/97 GTEIRILD-QQFTEVPTGEVGTIYVRNDSQFDGYTSGAA-----KDFHAG
Rv1427c|M.tuberculosis_H37Rv GTEIRILD-QQFTEVPTGEVGTIYVRNDSQFDGYTSGAA-----KDFHAG
MLBr_02546|M.leprae_Br4923 GVKVKILD-DSGKEVPRGQVGRIFVGNAFPFEGYTGGGG-----KQIIDG
MAB_2539c|M.abscessus_ATCC_199 GVSVRILR-EDRRPAAPGETGMIFVRGGLEYEGYTPDKATRPTAKEIVDG
. :::: * : .
Mvan_5064|M.vanbaalenii_PYR-1 WIRTTDIASIDEDGFVTLHGRADGAINRGGFKVLPEAVRRVLITHPDVRD
Mflv_1694|M.gilvum_PYR-GCK WIRTTDIASIDEDGFVTLHGRADGAINRGGFKVQPEEVRRVLISHPGVRD
MMAR_4025|M.marinum_M WVRTTDVARIDADGFVWIVGRADQAIIRGGFKVLPDDVGAALESHPAVAA
MUL_3890|M.ulcerans_Agy99 WVRTTDVARIDADGFVWIVGRADQAIIRGGFKVLPDDVRAALESHPAVAA
MSMEG_2217|M.smegmatis_MC2_155 WMRTTDLARIDADGFLWVLGRADQAIIRGGFKVMPDDVRAALESHPAVLG
TH_3166|M.thermoresistible__bu WVRTTDLGRLDSDGFLFVDGRTDDAIIRGGFKISPADIANALRSHPAVRD
MAV_2596|M.avium_104 WITTSDLGHLDEDGFLYIDGRADDVIVRGGFKVSPETVVRTLRAHPAVAD
Mb1462c|M.bovis_AF2122/97 FMSSGDVGYLDENGRLFVVGRDDEMIVSGGENIYPIEVEKTLATHPDVAE
Rv1427c|M.tuberculosis_H37Rv FMSSGDVGYLDENGRLFVVGRDDEMIVSGGENIYPIEVEKTLATHPDVAE
MLBr_02546|M.leprae_Br4923 LLSSGDVGYFDEHDLLYISGRDDEMIVSGGENVFPAEVEDLISGHPEVVE
MAB_2539c|M.abscessus_ATCC_199 HVNTGDMGHFDADGRLYIDGRSDDMIVSGGENVFPGEVEDRLTTYPGITD
: : *:. :* .. : : ** * * ** :: * : : :* :
Mvan_5064|M.vanbaalenii_PYR-1 ACVVGVPDARLGQVPFAAIEVSAGTAAPSDGELRELVRQSLPVYNVPVAF
Mflv_1694|M.gilvum_PYR-GCK ACVVGVPDARLGEVPFAAIEVVPGADTPSDDELESLVRQELPVYAVPVAF
MMAR_4025|M.marinum_M AAVVGRSDQRLGQTPVAMVELRG-SADIGGAELVEYLRTRLSGYEIPTQI
MUL_3890|M.ulcerans_Agy99 AAVVGRSDQRLGQTPVAMVELRG-SADIGGAELVEYLRTRLSGYEIPTQI
MSMEG_2217|M.smegmatis_MC2_155 ASVIGVADERLGETPVAMVELRG-PATP--DELAGYLQGRLARYEIPTRI
TH_3166|M.thermoresistible__bu AGVTGLPDPRLGAVPVAAVELAG-GAQAEPDELLDFLRQRLTRYQVPARL
MAV_2596|M.avium_104 AAVAPMPDQRLGQIPVAAVELRP-GAAADGEALREHCRATLTPYEVPARI
Mb1462c|M.bovis_AF2122/97 AAVIGVDDQQYGQRLAAFVVLKPGVSAT-PETLKQHVRDNLANYKVPRDI
Rv1427c|M.tuberculosis_H37Rv AAVIGVDDQQYGQRLAAFVVLKPGVSAT-PETLKQHVRDNLANYKVPRDI
MLBr_02546|M.leprae_Br4923 ATAIGVDDKEWGARLRAFVVKKQDATID-EDAIKAYVRDHLARYKVPREV
MAB_2539c|M.abscessus_ATCC_199 AVVIGVPDDDFGQVLHAFVVAADGAPAPSEEVLKTHVRQGLERYKVPKRF
* . * * * : : : * * :* .
Mvan_5064|M.vanbaalenii_PYR-1 AAVEELPRNPALKVSLPAVAALYSGHTGD--------
Mflv_1694|M.gilvum_PYR-GCK TMVDALPRNPALKVSLPAVAALYGAV-----------
MMAR_4025|M.marinum_M SIVAEIPRTASGKPDLGAIRHYFGAAGSRTDHGG---
MUL_3890|M.ulcerans_Agy99 SIVAEIPRTASGKPDLGAIRHYFGAAGSRTDHGG---
MSMEG_2217|M.smegmatis_MC2_155 TIVDAIPRTPSGKADLRAVRDHLESGG----------
TH_3166|M.thermoresistible__bu LVLDELPRTPSLKVSQPALRELFEQEPT---------
MAV_2596|M.avium_104 FVVDELPRGAALKVDRRRLIALLEDLGVPIGQPAAAG
Mb1462c|M.bovis_AF2122/97 AVLDELPRGITGKILRTELQSRVGS------------
Rv1427c|M.tuberculosis_H37Rv AVLDELPRGITGKILRTELQSRVGS------------
MLBr_02546|M.leprae_Br4923 IFLEELPRNPTGKVLKRELRDFEVH------------
MAB_2539c|M.abscessus_ATCC_199 IVLDEIPRNASGKVLRAKLNSPGAC------------
: :** : * :