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MQDHGEVLAAVRTGMIPAHVYNDKQIFSLEKERLFSRAWLFVAHESEIPQPGDYVVRQVLQDSFIIARDS AGEVRVMFNMCLHRGMQVCRAEMGNASNFRCPYHGWSYRNDGRIIGLPFHQEAYGGDAGFNKTGQTLLPA PSVASYNGLIFLSMDPDAESLEDYLGDFRFYLDFYTRQGPNGLEVQGPQRWRVKANWKIAAENFAGDMYH TPQTHTSVVEIGLFREPKAHKRKDGATYWAGRGGGTTYKLPEGSFEDRMSYVGYPAEMISRAKATWTEQQ RQVVGADGFMISAATCFPNISFVHNWPKVEDGEHVLPFISIRVWQPISENETEVLSWFAVDSDAPEAFKA ASYKAYLMCFGSTGMFEQDDVENWVSLTNTAGGSMARRLRLNSRMGLLADDARVVDTLSSAQFHGPGYAQ LGYNENNQRQLLRLWADYLDMPPLRVDPATVLTDNPQGIEPMVQTNGGAVAGIDSESATTSVTL
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. vanbaalenii PYR-1 | Mvan_0463 | - | - | 100% (484) | ring hydroxylating dioxygenase, alpha subunit |
| M. vanbaalenii PYR-1 | Mvan_0488 | - | 1e-92 | 41.40% (442) | ring hydroxylating dioxygenase, alpha subunit |
| M. vanbaalenii PYR-1 | Mvan_0546 | - | 9e-89 | 40.84% (453) | ring hydroxylating dioxygenase, alpha subunit |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb3186c | - | 1e-26 | 33.01% (209) | dioxygenase |
| M. gilvum PYR-GCK | Mflv_0685 | - | 0.0 | 97.93% (484) | ring hydroxylating dioxygenase, alpha subunit |
| M. tuberculosis H37Rv | Rv3161c | - | 1e-26 | 33.01% (209) | dioxygenase |
| M. leprae Br4923 | - | - | - | - | - |
| M. abscessus ATCC 19977 | MAB_4370c | - | 9e-54 | 31.21% (439) | putative large terminal subunit of phenylpropionate dioxygenase |
| M. marinum M | MMAR_0307 | - | 3e-23 | 28.63% (248) | dioxygenase |
| M. avium 104 | MAV_3509 | - | 0.0 | 75.17% (435) | phthalate dioxygenase large subunit |
| M. smegmatis MC2 155 | MSMEG_1908 | - | 5e-41 | 28.34% (374) | benzoate 1,2-dioxygenase alpha subunit |
| M. thermoresistible (build 8) | TH_2976 | - | 2e-37 | 31.03% (377) | PUTATIVE dioxygenase large subunit |
| M. ulcerans Agy99 | MUL_3820 | - | 3e-18 | 34.46% (177) | ring-hydroxylating dioxygenase, large terminal subunit |
CLUSTAL 2.0.9 multiple sequence alignment
Mvan_0463|M.vanbaalenii_PYR-1 ----------MQDHG----EVLAAVRTGM--------IPAHVYNDKQIFS
Mflv_0685|M.gilvum_PYR-GCK ----------MQDHG----EVLEAVRTGM--------IPAHVYNDKQIFS
MAV_3509|M.avium_104 ----------MAGPDGGLLGVLENVRRGM--------IPAHIYNDPELFA
Mb3186c|M.bovis_AF2122/97 --------MLSTDNRAELGDILTDIGDYLDDNPPALSLPPAAYTSSELWQ
Rv3161c|M.tuberculosis_H37Rv --------MLSTDNRAELGDILTDIGDYLDDNPPALSLPPAAYTSSELWQ
MAB_4370c|M.abscessus_ATCC_199 MASLPRPVAVAQGALCRPQALVRDIDVSGLVDSHGGLLDRVIFTDGELYR
TH_2976|M.thermoresistible__bu --------------------LIRPDRVHG-----------SLYTDPAIFA
MSMEG_1908|M.smegmatis_MC2_155 ---MTTHLSPDHLENVLADAVIEDPAAGVYR------ANRRIFTDDEIFE
MUL_3820|M.ulcerans_Agy99 -------MDRDQLIDLTRRALKLARDKTTDLAPHQHAVDAREYTSLDRHE
MMAR_0307|M.marinum_M --------MTDHNDGRAEPMDAAHHDDVEATLTGAVTFGVDAYTCADYAR
:.
Mvan_0463|M.vanbaalenii_PYR-1 LEKERLFSRAWLFVAHESEIPQPGDYVVRQVLQDSFIIARDSAGEVRVMF
Mflv_0685|M.gilvum_PYR-GCK LEKERLFSRAWLFVAHESEIPQPGDYVVRQVLQDSFIVARDSAGEVRVMF
MAV_3509|M.avium_104 LEKRRLFARAWTFVGHESEIPHDGDYMVRRVLDDSFIITRGSDGRVRALF
Mb3186c|M.bovis_AF2122/97 LERERIFNRSWMLVAHVDQLAKTGDYVTVSVAGEPVMVVRDVDGQLHALS
Rv3161c|M.tuberculosis_H37Rv LERERIFNRSWMLVAHVDQVAKTGDYVTVSVAGEPVMVVRDVDGQLHALS
MAB_4370c|M.abscessus_ATCC_199 QELRRVFAPSWLFLAHADQFHRPGDFFTTYMGEDPVIVSMDKNRRIRAFL
TH_2976|M.thermoresistible__bu EELRKIWYRTWVFVGHESEVSQPNDYVRKKLGLQDVIMTRDRDGEIHLLL
MSMEG_1908|M.smegmatis_MC2_155 LEMKHIFEGNWVYLAHESQVPNPGDYFTTYIGRQPVVITRDRDGDLHCLI
MUL_3820|M.ulcerans_Agy99 RDRAMVLD-SPQLVGYSSELPGPDTYRTKTIMGRSILLTRAADRAVRAFD
MMAR_0307|M.marinum_M AERDKLWRKVWQQVGRVEDLPKVGSYLTYDILDDSIVVVRSAADTFKAHH
: : :. .:. . : : .:: .:
Mvan_0463|M.vanbaalenii_PYR-1 NMCLHRGM------QVCRAEMGNASNFRCPYHGWSYRNDGRIIGLPFHQE
Mflv_0685|M.gilvum_PYR-GCK NMCLHRGM------QVCRAEMGNASNFRCPYHGWSYRNDGRIIGLPFHQE
MAV_3509|M.avium_104 NMCLHRGM------QVCRAELGNASNFRCPYHGWTYRNDGRLTGLPFHRE
Mb3186c|M.bovis_AF2122/97 PICRHRLM------LMVEPGAGRIDTLTCQYHLWRYGLDGRLRGAPHMAA
Rv3161c|M.tuberculosis_H37Rv PICRHRLM------LMVEPGAGRIDTLTCQYHLWRYGLDGRLRGAPHMAA
MAB_4370c|M.abscessus_ATCC_199 NSCRHRGA------RVCRADYGVTRGFTCTYHGWAYDLEGKLISVPNEEG
TH_2976|M.thermoresistible__bu NRCSHRGN------QVCADAKGNSSTFRCPYHGWTFRNTGELVGFPFFKG
MSMEG_1908|M.smegmatis_MC2_155 NACAHRGA------MICRRKTDNRTTLTCPFHGWTFRNDGTLLKVKDPDG
MUL_3820|M.ulcerans_Agy99 NVCLHRQS------RVATG-CGTALRFTCPYHGWTYDNGGRLVGLPGREG
MMAR_0307|M.marinum_M NVCMHRGRRLVDIPPGAKNVCGHKKSFVCGFHGWTYDQDGNCIHIPEKPD
* ** . : * :* * : *
Mvan_0463|M.vanbaalenii_PYR-1 AYGGDAGFNKTGQTLLPAPSVASYNGLIFLSMDPDA-ESLEDYLGDFRFY
Mflv_0685|M.gilvum_PYR-GCK AYGGDAGFNKAGQTLLPAPSVASYNGLIFLSMDPDA-ESLEEYLGDFRFY
MAV_3509|M.avium_104 AYGGDDGFVKD-QTLLPAPNFAGYNGLLFISLDPAA-PPLQDFLGDFRFY
Mb3186c|M.bovis_AF2122/97 NLD----FNRR-ECRLPQFAVATWNGLVWINLDADA-EPIAAHL---DLT
Rv3161c|M.tuberculosis_H37Rv NLD----FNRR-ECRLPQFAVATWNGLVWINLDADA-EPIAAHL---DLT
MAB_4370c|M.abscessus_ATCC_199 YPDS---FSTDDWGLVEVPHVDSYHGLIFGTWNPAP-VGLRESLGDMAWY
TH_2976|M.thermoresistible__bu YGTR-----KLDLPLGRVPRVDSYGGFVFGSFATEG-PTLREHLGAAAGE
MSMEG_1908|M.smegmatis_MC2_155 AGYPDTFNSNGSHNMTNVARFEGYRGFLFGSLNPDV-LPLTEHLGDTTTV
MUL_3820|M.ulcerans_Agy99 FPG-----IAVGSDGLTELPATEFAGFLWVSLHPAARLDVPAHLGPLAGE
MMAR_0307|M.marinum_M WQG----ALTPENTHLAAVNVDTWGGWIWINMDPAA-QPLRQYLEPAATM
: * :: . . : *
Mvan_0463|M.vanbaalenii_PYR-1 LDFY--TRQGPNGLEVQG--PQRWRVKANWKIAAENFAGDMYHTPQTHTS
Mflv_0685|M.gilvum_PYR-GCK LDFY--TRQGPNGLEVRG--PQRWRVKANWKIAAENFAGDMYHTPQTHTS
MAV_3509|M.avium_104 LDYY--TRQSAGGVELRG--PQRWRIRANWKIGAENFAGDMYHTPHTHAS
Mb3186c|M.bovis_AF2122/97 DDEF--AGYRLGEMVQVE--SWSHEWRANWKVAAEN-GHENYHVLGLHRQ
Rv3161c|M.tuberculosis_H37Rv DDEF--AGYRLGEMVQVE--SWSHEWRANWKVAAEN-GHENYHVLGLHRQ
MAB_4370c|M.abscessus_ATCC_199 MDAM--LDHDSEGTVVVGG-VHKWVLDGNWKLAAEQFATDWYHVNMSHAS
TH_2976|M.thermoresistible__bu IDRL--LRLSPEGKVALDAGWLRHRTRANWKLLVEN-ETDGYHPQFVHGG
MSMEG_1908|M.smegmatis_MC2_155 IDML--VDQSPDGLEVLRG-ASTYTYDGNWKVQAEN-GADGYHVTATHWN
MUL_3820|M.ulcerans_Agy99 LQSWGIGRWSPLGEKVLG-------SPIDWKLATDT-FAENYHFATVHRR
MMAR_0307|M.marinum_M LDPFRLQDMRCKWRKWLT-------FQCNWKVAMEA-FNETYHVATTHPQ
: :**: : : ** *
Mvan_0463|M.vanbaalenii_PYR-1 VVEIGLFREPKAHKRKDGATYWAGRGGGTTYKLPEGSFEDRMSYVGYPAE
Mflv_0685|M.gilvum_PYR-GCK VVEIGLFREPKANKRKDGATYWAGRGGGTTYKLPEGSFEDRMSYVGYPAE
MAV_3509|M.avium_104 IVDIGLFREPKAQKRKDGATYWAHRGGGTTYKLPPGGFEERMRYVGYPDE
Mb3186c|M.bovis_AF2122/97 TLEP---------------------------FVPGGGDLDVRQYSRWALR
Rv3161c|M.tuberculosis_H37Rv TLEP---------------------------FVPGGGDLDVRQYSRWALR
MAB_4370c|M.abscessus_ATCC_199 ALMVMAPQ-----------------GKAPKDEIVHRTGRQYVDPNGHGAG
TH_2976|M.thermoresistible__bu IFGVTGSTIG-------------PLYSDSSTAVTRDLGGGHSENDLRPEF
MSMEG_1908|M.smegmatis_MC2_155 YAATTSRRTCG--------------ESKNDTKALDAGSWGKSGGGYWSFP
MUL_3820|M.ulcerans_Agy99 TFAT----------------------------IARSNCTVFDSYGPHHRL
MMAR_0307|M.marinum_M FNKFGNFRGWAAAHG---------RHSNIGYDAPKDLAQTKSKIRLGTGA
Mvan_0463|M.vanbaalenii_PYR-1 MISRAKATWTEQQR-QVVGADGFMISAATCFPNISFVHNWPKVEDG--EH
Mflv_0685|M.gilvum_PYR-GCK MISRAKATWTEQQQ-QVVGTDGFMISAATCFPNISFVHNWPKVEDG--EH
MAV_3509|M.avium_104 MIGRIKQVWTPRQQ-RVVGEDGFMVSAATCFPNLSFVHNWPKVRDGRDDE
Mb3186c|M.bovis_AF2122/97 LRVPFTVPVEAKSL-QLNEVQKSNLVVLWTFPNSALAIAG--------ER
Rv3161c|M.tuberculosis_H37Rv LRVPFTVPVEAKSL-QLNEVQKSNLVVLWTFPNSALAIAG--------ER
MAB_4370c|M.abscessus_ATCC_199 FPVHPKNRFDARVV-HQWTDYGALRERLGEARVQGPLTNGHATVFPNLSY
TH_2976|M.thermoresistible__bu RKFAEPLRWFGTTE-SRVPDYVAAMRARYGAEADQILIEGSPHVMIFPNL
MSMEG_1908|M.smegmatis_MC2_155 HGHLCLWTWAANPQDRPLWDRLDELKARFGDAKGEFMVKGSRNLCLYPNV
MUL_3820|M.ulcerans_Agy99 IFPLNTILELENVPEGQWDPFHHMVVIYALFPNVVLSVTIANG-----EL
MMAR_0307|M.marinum_M DPRVSTAEMQRYTLEETNATTTRTLVDAALRLVEELPEDAPAGQVLDHWL
Mvan_0463|M.vanbaalenii_PYR-1 VLPFIS---IRVWQPISENETEVLSWFAVDSDAPEAFKAASYKAYLMCFG
Mflv_0685|M.gilvum_PYR-GCK VLPFIS---IRVWQPISENETEVLSWFAVDSDAPEAFKADSYKAYLMCFG
MAV_3509|M.avium_104 TLPFIS---IRLWQPISENETEVCSWFAVDSAAPAHYKQDSYKAYLMCFG
Mb3186c|M.bovis_AF2122/97 VVWFG-------FIPQSIDRVQVLGGVLTTPELAADAAATAQTSQFVMA-
Rv3161c|M.tuberculosis_H37Rv VVWFG-------FIPQSIDRVQVLGGVLTTPELAADAAATAQTSQFVMA-
MAB_4370c|M.abscessus_ATCC_199 LPVNGS---VRIWHPKGPDKMEVWAWTIVDRSMPEEVRDAQRLYNLRTFG
TH_2976|M.thermoresistible__bu FIAEIQ---VFNIQPVAVNECVQYSTAVQLVGAPELNRR-MVSQCIGSVG
MSMEG_1908|M.smegmatis_MC2_155 YVMDQFSTQIRHFRPIAPDKTEVTIYCIAPKGESAAARAHRIRQYEDFFN
MUL_3820|M.ulcerans_Agy99 FRIYPG------DRPGSSITVHQNSTPLDLSDESVATGAQAVFDYAHAT-
MMAR_0307|M.marinum_M TSARRDDAARGVLWPTIDPEHLAQSGTAWQIFPNFQIGQGLTTALCYSAR
*
Mvan_0463|M.vanbaalenii_PYR-1 STGMFEQDDVENWVSLTNTAGGSMARRLRLNSRMGLLADDARVVDTLSSA
Mflv_0685|M.gilvum_PYR-GCK STGMFEQDDVENWVSLTNTAGGSMARRLRLNSRMGLLADDARVVDTLSSA
MAV_3509|M.avium_104 STGMFEQDDVENWVSLTTTAGGSMARRLLLNSRMGLYDDGRPVVEALAPS
Mb3186c|M.bovis_AF2122/97 ---MINDEDRLGLEAVQVGAGSRFAERGHLSSKE----------------
Rv3161c|M.tuberculosis_H37Rv ---MINDEDRLGLEAVQVGAGSRFAERGHLSSKE----------------
MAB_4370c|M.abscessus_ATCC_199 PTGIFEQDDGENWSEVQSISRGFVTNSVALNYQMGLDSEREDG-------
TH_2976|M.thermoresistible__bu PAGMLLADDTEMYERNQQGLEALTPEWLDIRRGLNREHVDDHG-------
MSMEG_1908|M.smegmatis_MC2_155 ASGMATPDDLEEFRSCQLTFRAQAAPWNDMSRGAQHWLSGPDPVAESLG-
MUL_3820|M.ulcerans_Agy99 ----VRDEDYRLAETLQTNLESGARQRLVFGRNE----------------
MMAR_0307|M.marinum_M PHGYDPDQCVFEASCYELYPKGEEPHTEWVYAPPDDPGWRSVLPQDFSNM
: . .
Mvan_0463|M.vanbaalenii_PYR-1 QFHGPGYAQLGYNENNQRQLLRLWADYLDMPPLRVDPATVLTDNPQGIEP
Mflv_0685|M.gilvum_PYR-GCK QFHGPGYAQLGYNENNQRELLRLWADYLDMPPLRVDPATVLTDNPQGIEP
MAV_3509|M.avium_104 AFHGPGRAQVGYNEHNQRALLAMWADYLQE--------------------
Mb3186c|M.bovis_AF2122/97 ---WPG--MLAFYRNLAMALVGDHPGAS----------------------
Rv3161c|M.tuberculosis_H37Rv ---WPG--MLAFYRNLAMALVGDHPGAS----------------------
MAB_4370c|M.abscessus_ATCC_199 --RHPGLTSELYSDAAGRSFYARWRDLMNTKAWHEEHV------------
TH_2976|M.thermoresistible__bu -----FTVGGPTDETGMRAFWSEYRRLMEAE-------------------
MSMEG_1908|M.smegmatis_MC2_155 ---MHGIVSSGLRNEDEGLYPVQHGYWLETLRRAVTSNPTEH--------
MUL_3820|M.ulcerans_Agy99 --------------PGLQHRHITWAQALETPG------------------
MMAR_0307|M.marinum_M AAVQQGMKSGGFGGPKPNPYMERSTANLHRNLAEYMGTGAPREL------
Mvan_0463|M.vanbaalenii_PYR-1 MVQTNGGAVAGIDSESATTSVTL
Mflv_0685|M.gilvum_PYR-GCK MVQTNGGAVAGIDSESATTSVTL
MAV_3509|M.avium_104 -----GDACENR-----------
Mb3186c|M.bovis_AF2122/97 -----------------------
Rv3161c|M.tuberculosis_H37Rv -----------------------
MAB_4370c|M.abscessus_ATCC_199 -----------------------
TH_2976|M.thermoresistible__bu -----------------------
MSMEG_1908|M.smegmatis_MC2_155 -----------------------
MUL_3820|M.ulcerans_Agy99 -----------------------
MMAR_0307|M.marinum_M -----------------------