For questions or suggestions e-mail us at: ioerger@cs.tamu.edu
ADMTEPHSAVIARLSADFAAISHQLTRVSGDLAALDRILTDRAPAAAAPRPAPQPAAYWPQYPPQPAPFR PWPQAPAPAPTQALTPAPRKERSEGWIGKLLAVAGVAVTLIGVVLLLVLAAQAGILRPEFRVSAGGALAA ALVGAGWWLNTRPGGRVGAIALVATGVAAAYMDVIAVTTIYDWVPAPAGLVLAAVIAGAGLTLARRWDSE HLGLLVLVPLIVLAPIVTDGVSLLLVGFMLALAAVSLPVQLGKDWIWLHAARIAAAVLPLLVALAARHID SREDLWLAGACGIAAVIALTAALILLPGTGNRVVMALLTVAGIVPVLCVNLAVDRVVAALMAAALAAALL AVALLADRLPGVDGVVRRVFAVTSAIAALIAVTTAFDGRVGGPVLLAMAVVVAIAVGRDVVGRWVAIGFA VIGAAAHLSYAPLAVLLQPVEVSTATGISTLVSSLLLATAAVTIVWMWDRTETALWVGAAVVTVYAVTSC TVTAGMLVIGGDGGFYAGHMVATILWIAMAAVLFWYAARLPREDRSVPLGGGLGLVAAAMAKLFLFDLGT LDGIFRVVVFIVVGLTLLGIGAGYARLLERQDKQAATRTFDTT*
Operon Prediction Model: Genebank
Paralogs
species | id | gene | e-value | identity (len) | annotation |
M. gilvum PYR-GCK | Mflv_3578 | - | - | 100% (604) | hypothetical protein Mflv_3578 |
M. gilvum PYR-GCK | Mflv_0954 | - | 3e-06 | 27.49% (342) | hypothetical protein Mflv_0954 |
M. gilvum PYR-GCK | Mflv_1175 | - | 6e-06 | 23.52% (625) | cell wall arabinan synthesis protein |
Closest Orthologs (e-value cutoff: 1e-4)
species | id | gene | e-value | identity (len) | annotation |
M. bovis AF2122 / 97 | Mb0298 | - | 1e-06 | 23.24% (413) | transmembrane protein |
M. tuberculosis H37Rv | Rv0290 | - | 4e-07 | 23.49% (413) | transmembrane protein |
M. leprae Br4923 | MLBr_02582 | - | 3e-05 | 24.80% (371) | hypothetical protein MLBr_02582 |
M. abscessus ATCC 19977 | MAB_4509c | - | 1e-75 | 36.92% (577) | hypothetical protein MAB_4509c |
M. marinum M | MMAR_3960 | - | 1e-178 | 56.62% (627) | hypothetical protein MMAR_3960 |
M. avium 104 | MAV_4116 | - | 2e-09 | 28.49% (358) | hypothetical protein MAV_4116 |
M. smegmatis MC2 155 | MSMEG_3241 | - | 1e-180 | 57.86% (598) | hypothetical protein MSMEG_3241 |
M. thermoresistible (build 8) | TH_4594 | - | 1e-124 | 60.31% (393) | PUTATIVE putative conserved hypothetical protein |
M. ulcerans Agy99 | MUL_1004 | - | 3e-05 | 56.52% (46) | hypothetical protein MUL_1004 |
M. vanbaalenii PYR-1 | Mvan_2837 | - | 0.0 | 74.35% (616) | hypothetical protein Mvan_2837 |
CLUSTAL 2.0.9 multiple sequence alignment Mb0298|M.bovis_AF2122/97 -------------------------------------------------- Rv0290|M.tuberculosis_H37Rv -------------------------------------------------- Mflv_3578|M.gilvum_PYR-GCK MADMTEPHSAVIARLSADFAAISHQLTRVSGDLAALDRILTD-----RAP Mvan_2837|M.vanbaalenii_PYR-1 ---MTEPHTAVITRLSADFAAISRQLARVSTDLVELDRILAD-----RPQ TH_4594|M.thermoresistible__bu -------------------------------------------------- MMAR_3960|M.marinum_M ---MTESQHAVISRLSAEFDSLARQMARVSGELSQLDRLISDSVGAPQPA MSMEG_3241|M.smegmatis_MC2_155 ---MTEPQRAVIARVTADLTAVSAYLNRMAGDLATLDRLVAQQSAAPRPE MAB_4509c|M.abscessus_ATCC_199 ----MFTSHRTASTLRGMSDPQLQQMTATLDELDGRERALRAEVDALRGR MAV_4116|M.avium_104 -------------------------------------------------- MLBr_02582|M.leprae_Br4923 ---MSRELYHRSMSWSRPSYALGMALLVVGPLMRPGYLLLRDAVSTPRSY MUL_1004|M.ulcerans_Agy99 -------------------------------------------------- Mb0298|M.bovis_AF2122/97 -------------------------------------------------- Rv0290|M.tuberculosis_H37Rv -------------------------------------------------- Mflv_3578|M.gilvum_PYR-GCK AAAAPR-------------------PAPQPAAYWPQYP-PQPAPFRPWPQ Mvan_2837|M.vanbaalenii_PYR-1 SAPAPWPQPVSVP-------SPVPVPSPVPVAQPVPVPSPAPVPQPVAPY TH_4594|M.thermoresistible__bu -------------------------------------------------- MMAR_3960|M.marinum_M AAAPPQPQPQPVAPYWYPYWQQWAPVAPAPAAAPPRQYAPPPTARPAPQG MSMEG_3241|M.smegmatis_MC2_155 AVAPQWS--------------------PQAPQQPPRQWTPQPVAPVAPVA MAB_4509c|M.abscessus_ATCC_199 MVEWTQAEYLKAQR------------YWDDYRRAQGVAQAVAPAPVVMPP MAV_4116|M.avium_104 -------------------------------------------------- MLBr_02582|M.leprae_Br4923 LSDAALG-------------------LTSAPRSTPQDFAVAMASHLVDGG MUL_1004|M.ulcerans_Agy99 -------------------------------------------------- Mb0298|M.bovis_AF2122/97 -----------------------------------MSGTVMQIVRVAILA Rv0290|M.tuberculosis_H37Rv -----------------------------------MSGTVMQIVRVAILA Mflv_3578|M.gilvum_PYR-GCK APAPAPTQALTPAP---RKERSE-GWIGKLLAVAGVAVTLIGVVLLLVLA Mvan_2837|M.vanbaalenii_PYR-1 RPAYPAYPAYPPASARVRKERSD-GWIGKLLAVAGVAVTLIGVVLLLVLA TH_4594|M.thermoresistible__bu -------------------------------------------------- MMAR_3960|M.marinum_M PPAPAPAAAPPVSAPRRPAESTESGWIGKLLAVAGVAVTLTGVALLLVLA MSMEG_3241|M.smegmatis_MC2_155 VPARQPDTPP--------KPRSE-GWIGKVLAMAGVAVTLVGVVSLLVLA MAB_4509c|M.abscessus_ATCC_199 ARMPVPARAPHPAPPQLEPWWMREGFASKAMALAGAVVTLAGVVMLLVLA MAV_4116|M.avium_104 ---------------------------------------MPKRSLLAQAR MLBr_02582|M.leprae_Br4923 IVVKSALVLGLWLAGWGAARLVVTALPSAGVAGQFVASTLAVWNPYVAER MUL_1004|M.ulcerans_Agy99 --------------------------------------------MASTWS Mb0298|M.bovis_AF2122/97 DSR-----LTEMALPAELPLREILPAVQRLVVPSAQNGDGGQADSGAAVQ Rv0290|M.tuberculosis_H37Rv DSR-----LTEMALPAELPLREILPAVQRLVVPSAQNGDGGQADSGAAVQ Mflv_3578|M.gilvum_PYR-GCK AQAGILRPEFRVSAGGALAAALVGAGWWLNTRPGGRVGAIALVATGVAAA Mvan_2837|M.vanbaalenii_PYR-1 AQAGILRPEFRVAAGAVLAGALVAAGWWLNGRPGGRVGAIALVATGVAAA TH_4594|M.thermoresistible__bu -------------------------------------------------- MMAR_3960|M.marinum_M AQAGLLRPEIRVAGGTALSLALVGVAVRLRGRPGGRIGAIALAATGIATA MSMEG_3241|M.smegmatis_MC2_155 AQAGILRPEVRVAAGAALAVALVAVAMWLDRRPGGRVGAVALAATGVAAA MAB_4509c|M.abscessus_ATCC_199 AKSGYFGPVPRMVSGAALAGGLVALGIRVYSRPGGRVGGIATAATGFAAA MAV_4116|M.avium_104 PESGSGEYVLGTWRAAALSMAVIAPAAWYG------LGALLGAAVMIPVI MLBr_02582|M.leprae_Br4923 LLQGHWSLLVGYGCLPWVAEAMLMLRSSDNASRPGLLGFFALACWIALAG MUL_1004|M.ulcerans_Agy99 LSKGLAAVVVTSAAAFGLCPAAAADPAAPQPNPNPQLPGLPALAQLSP-- Mb0298|M.bovis_AF2122/97 LS--------------------LAPVGGQPFSLDASLDTVGVVDGDLLVL Rv0290|M.tuberculosis_H37Rv LS--------------------LAPVGGQPFSLDASLDTVGVVDGDLLVL Mflv_3578|M.gilvum_PYR-GCK YMDVIAVTTIYDWVPAPAGLVLAAVIAGAGLTLARRWDSEHLGLLVLVPL Mvan_2837|M.vanbaalenii_PYR-1 YMDVIAVTTIYEWTSAPAGLVFASLIAGGGLTLARRWDSEHLGLLVLVPL TH_4594|M.thermoresistible__bu -------------------------------MLARRWDSEHLGLLVLVPL MMAR_3960|M.marinum_M YLDVIAATTIYQWVPAPAGLVLAALVGGAGLALARRWDSEHLGLLVLVPL MSMEG_3241|M.smegmatis_MC2_155 YMDVIAVTAIYEWVPAPVGLALAGVVGACGLMLARWWGSEQLGLLVFVPL MAB_4509c|M.abscessus_ATCC_199 FFDVLALTVIYDRIPVVAGLVLGLGIAGVGLVLARHWNSQPFASGVVAAI MAV_4116|M.avium_104 YA----------------------------LARLRRHAPQARSTAELIGA MLBr_02582|M.leprae_Br4923 LT--------------PTGLMLAATVALICVAVPVEGPGEPRPRWLCAAA MUL_1004|M.ulcerans_Agy99 -----------------------------IIQQAAGNPEQATQLLMAAAQ Mb0298|M.bovis_AF2122/97 QPVPAGPAAPGIVEDIADAAMIFSTSRLKPWGIAHIQRGALAAVIAVALL Rv0290|M.tuberculosis_H37Rv QPVPAGPAAPGIVEDIADAAMIFSTSRLKPWGIAHIQRGALAAVIAVALL Mflv_3578|M.gilvum_PYR-GCK IVLAPIVTDGVSLLLVGFMLALAAVSLPVQLGKDWIWLHAARIAAAVLPL Mvan_2837|M.vanbaalenii_PYR-1 IGLAPIVTDGISLLLVGFMLALAAVSLPVQLGKDWIWLHAARIAAAALPV TH_4594|M.thermoresistible__bu VLLAPVITDGITVVLIGFMLALGAASLPVQLGRDWIWLHAARTASVTLPI MMAR_3960|M.marinum_M IGLAPVLTRGADLLLVSFMLALSAAALPVQLGKDWVWLHAARVAAPTVPL MSMEG_3241|M.smegmatis_MC2_155 IALAPVITDGVTLLLIGFMLALSAASLPVQFGRDWFWLHAARTAAVTIPL MAB_4509c|M.abscessus_ATCC_199 TVLAPFLTDGFTVELAAFALILLIASLTAQVGRNWPVLHAFRTVGVSLTL MAV_4116|M.avium_104 MLGRKAGTAAGIVQLLAYLVLAAQFAATLGLQLLQLLSPGDDPAAIVSWL MLBr_02582|M.leprae_Br4923 TLGSALGAALPWLTASAVGTSLTAHTVANTLGVTVFAPRAEPGLGTLASL MUL_1004|M.ulcerans_Agy99 AFAHNPAAPTESRNVASSVNQFVQEPVNPAPGSPPLGVPAPAAAPVEVAP : . . . Mb0298|M.bovis_AF2122/97 ATGLTVTYRVATGVLAGLLAVAGIAVASALAGLLITIRSPRSGIALSIAA Rv0290|M.tuberculosis_H37Rv ATGLTVTYRVATGVLAGLLAVAGIAVASALAGLLITIRSPRSGIALSIAA Mflv_3578|M.gilvum_PYR-GCK LVALAARHID-SREDLWLAGACGIAAVIALTAALILLPGTGNRVVMALLT Mvan_2837|M.vanbaalenii_PYR-1 LTALLARHFESARQDLWLAGASGIAAAVALVAALILLPGTKNRAAMALLT TH_4594|M.thermoresistible__bu LGALLGASFAQDRVPALTA-ACGIAAVLALAAALLLAPSTRNHTALGLLT MMAR_3960|M.marinum_M LIALVAAGKH--DNTWLLGAACGVAAILAIVSGLIVLPGGKNAAALAVLT MSMEG_3241|M.smegmatis_MC2_155 LVALVSAAIG-GREDLRLALVCALAAVPALVGGVTVSRFSTRPVATTVVS MAB_4509c|M.abscessus_ATCC_199 LTAIHVSYTA----SLALLAMSVMALLVTLVGSLWLLTGEHDDITSSVMI MAV_4116|M.avium_104 PVGAAAAALVVGATACWASTRAVASLAMPLVLAGLLLYVCLAVAVTALVT MLBr_02582|M.leprae_Br4923 ASLGGIWNGEAVPTSRTTLFAVLSATVLLGVVVAGLPVAVRRPAVVPLLV MUL_1004|M.ulcerans_Agy99 PASAPVPHVPAPGAEPGTLAHLPAGIDPAHAAGPAPVAAPAGAPPASAPA . . Mb0298|M.bovis_AF2122/97 LVPIGAALALAVPGKFGPAQVLLGAAGVAAWSLIALM-------IPSAER Rv0290|M.tuberculosis_H37Rv LVPIGAALALAVPGKFGPAQVLLGAAGVAAWSLIALM-------IPSAER Mflv_3578|M.gilvum_PYR-GCK VAGIVPVLCVNLAVDRVVAALMAAALAAALLAVALLA--DRLPGV-DGVV Mvan_2837|M.vanbaalenii_PYR-1 AAGVLPVLCVSLAVDRVVAALMAATLAAALLAIVLFG--ERLPGV-AGVV TH_4594|M.thermoresistible__bu AAGTLPVLCVSWAERPAVSALMAAALSAALLAMAAGAGLIRVELVRTGPL MMAR_3960|M.marinum_M VVGTLPVLAAAVAVDRVFAALLAGTLAAALLAIVLVG--SELPTI----F MSMEG_3241|M.smegmatis_MC2_155 ALGTVPLLCVSATADRVPAVLLIAGLAAAALAVAAIG--DRLPGI-GAPV MAB_4509c|M.abscessus_ATCC_199 AVASSPVLYGALFFPVWPLGVLVPVGVAVVMGAVLLL-----VGALPVHA MAV_4116|M.avium_104 LSTDPVVIGSAATPRPLSGQLVGFGLGMVGVELLTVR------------T MLBr_02582|M.leprae_Br4923 LAAVAVATPAALATGPGIDMLKAVVNAVPGLGVLRDG------------Q MUL_1004|M.ulcerans_Agy99 PAAAAPASAPGTAPAPAAAPGPAPAAPAPAPAAAAPAP------------ Mb0298|M.bovis_AF2122/97 ERVVAFFTAAAVVGASVALAAG---AQLLWQLPLLSIGCGLIVAALLVTI Rv0290|M.tuberculosis_H37Rv ERVVAFFTAAAVVGASVALAAG---AQLLWQLPLLSIGCGLIVAALLVTI Mflv_3578|M.gilvum_PYR-GCK RRVFAVTSAIAALIAVTTAFDGRVGGPVLLAMAVVVAIAV--GRDVVGRW Mvan_2837|M.vanbaalenii_PYR-1 RQVFSATSAVAALIAVTVAFDGMIAGPVLLAMAVVVALVG--RRDEVARW TH_4594|M.thermoresistible__bu RRILSVWAAVAALIAVTVAFDAPVAAPVLLAMATVVAVAG--RHDRTARW MMAR_3960|M.marinum_M TRVLAAWSAISALVAVTVAFDGHVEGPILLALAIVVAIAG--RHDRMALW MSMEG_3241|M.smegmatis_MC2_155 RRVWAATSAAAALIAVLVAFDGTVAAPVLLAMSIAIAVGG--QRDPVGRC MAB_4509c|M.abscessus_ATCC_199 RITVAAVAGVALLQASVDGARDALLAVVLLAIALSCCAIGYQLRDRVALV MAV_4116|M.avium_104 PRIARPGRSMSLATAVVA------AAAVVLWVGDHRGVAG------PWRW MLBr_02582|M.leprae_Br4923 KWVALAVPGYSLAGAGAVVTLGRWLRPSRPLSPVVTALAC--CLALILAL MUL_1004|M.ulcerans_Agy99 --APAPAAPPAAPVAAPVAAPAPVPAPAPAAAPAPEAAAP-------APA : * Mb0298|M.bovis_AF2122/97 QAAQLSALWARFPLPVIPAPGDPTPSAPPLRLLEDLPRRVRVSDAHQSGF Rv0290|M.tuberculosis_H37Rv QAAQLSALWARFPLPVIPAPGDPTPSAPPLRLLEDLPRRVRVSDAHQSGF Mflv_3578|M.gilvum_PYR-GCK VAIGFAVIGAAAHLSYAPLAVLLQPVEVSTATGISTLVSSLLLATAAVTI Mvan_2837|M.vanbaalenii_PYR-1 AAIGFAVVGAGVHLSYSPPSMLLEATRISAAAGISTLVSAVLLAAAAVAV TH_4594|M.thermoresistible__bu IALGFAVVGGLYYLGVAPPYRVLRPIAVAAPTAVAVLIASVLLICCVVAI MMAR_3960|M.marinum_M AAAGFGIVGLGLYYSYAPLHALLTATVMPTSIAVSTLAASLLSIAFVVAM MSMEG_3241|M.smegmatis_MC2_155 AAIGFALIGAMFYLDHAAPAMLVEATPLDGPTVASVVIGSVMLIGAAAAN MAB_4509c|M.abscessus_ATCC_199 LGQVFGGLGAAVYLAYVRPELLTDSASAVLYAGPLLVIASVLMAATVGMV MAV_4116|M.avium_104 SATMLAEAVPEFYTDEGRRWMAAAGIALAVAAALVTGWAATRAAAGLAAM MLBr_02582|M.leprae_Br4923 PDLAWGVWGKVQPVHYPSGWAAVAATINDRGEGP--GWVAVLPAGTMRRF MUL_1004|M.ulcerans_Agy99 PAPAPAAAPGFGPDAPPTQDFMYPSIGTNCLADGSNIIATALSVAGPATI . Mb0298|M.bovis_AF2122/97 IAAAVLLSVLGSVAIAVRPEALSVVGWYLVAATAAAATLRARVWDSAACK Rv0290|M.tuberculosis_H37Rv IAAAVLLSVLGSVAIAVRPEALSVVGWYLVAATAAAATLRARVWDSAACK Mflv_3578|M.gilvum_PYR-GCK VWMWDRT----ETALWVGAAV---VTVYAVTSCTVTAGMLVIGGDGGFYA Mvan_2837|M.vanbaalenii_PYR-1 VWAWDHTGGATGLAIWIVPAA---VVVYAVTSFTVTAGMLIGGGDGGFYA TH_4594|M.thermoresistible__bu VWTWRAHVPAAGPLPWLGAAT---VLLYAVTTFTVTAGVLAGGVGGGFFA MMAR_3960|M.marinum_M SRACAGIGGQNRDIRRILAAVGGVLVIYAVTAFTVTAGVLIAGTEAGFLA MSMEG_3241|M.smegmatis_MC2_155 GWTWSRTVSDTEVVRLVWVAVS-AVIGYAATALTVTVGVALGGAEVGFLA MAB_4509c|M.abscessus_ATCC_199 LATMARVGWASPQSAPTRAVLAGVSMLYSGTAAVVLSGTALLGNNDGFLL MAV_4116|M.avium_104 RGATPNAG-----LRLTVMGLVAVVAVVVSAHGMRSIGMVILG------- MLBr_02582|M.leprae_Br4923 SWSGTAP-VLDPLPRWVRDDVLTTGDLIISGVMVAGEGNHARAAQDLLLS MUL_1004|M.ulcerans_Agy99 PTPGPGPG----------------QTAYVFTAVGTPG------------- Mb0298|M.bovis_AF2122/97 AWLLAQPYLVAGVLLVFYTTTGRY-------VAAFGAVLVLAVLMLAWVV Rv0290|M.tuberculosis_H37Rv AWLLAQPYLVAGVLLVFYTATGRY-------VAAFGAVLVLAVLMLAWVV Mflv_3578|M.gilvum_PYR-GCK GHMVATILWIAMAAVLFWYAARLP---REDRSVPLGGGLGLVAAAMAKLF Mvan_2837|M.vanbaalenii_PYR-1 GHMAATICWIAMAAALFGYAARLP---REDRSMLIGGGLGLVAAAMAKLF TH_4594|M.thermoresistible__bu GHMAATICWIGLAAALLWYAARRP---RADRAMPLGGGLALVAAAVAKLF MMAR_3960|M.marinum_M GHMAATICWIAVAAALFVYALRVAD--RERRTAPIGAGLALTGAATAKLF MSMEG_3241|M.smegmatis_MC2_155 GHMAATLSWIVAAALAFGYAARRP---GASRSVLIGGGLVLVAAATGKLF MAB_4509c|M.abscessus_ATCC_199 GHGLATVSWMAVSVALLLAGLRRY----RGRSWVTRTGFVLAAMAVAKLF MAV_4116|M.avium_104 ---AAPLLLMALYVLVAEANSRIP----GDSVVAWWVRLVMPALAVVAVV MLBr_02582|M.leprae_Br4923 GPNPSALTAAGVAWLVVESDTAGDMGASARTLAALQPTYRDDAIGLYRIG MUL_1004|M.ulcerans_Agy99 ---PAEVQRLPLNVTWVNLTTGKS------GSVTLKPRSDINPEGPTTLT : : Mb0298|M.bovis_AF2122/97 VALNPGIASPE---SYSLPLRRLLGLVAAGLDVSLIPVMAYLVGLFAWVL Rv0290|M.tuberculosis_H37Rv VALNPGIASPE---SYSLPLRRLLGLVAAGLDVSLIPVMAYLVGLFAWVL Mflv_3578|M.gilvum_PYR-GCK LFDLGTLDG-----IFRVVVFIVVGLTLLGIGAGYARLLERQDKQAATRT Mvan_2837|M.vanbaalenii_PYR-1 LFDLGTLDG-----IFRVAVFIVVGLILLGMGAGYARLLEKQDKKL---- TH_4594|M.thermoresistible__bu LFDLGTLDG-----IFRVVVFIVVGLVLLGTGGGYARWLTHAAGESHPAG MMAR_3960|M.marinum_M LFDLATLDG-----IFRVAAFIVVGLVLLAMGTGYARSLAR--------- MSMEG_3241|M.smegmatis_MC2_155 LFDLGTLDG-----MYRVVLFIVGGLVLLGMGAGYARFLAQQSDGRSDAQ MAB_4509c|M.abscessus_ATCC_199 LFDLATLDG-----VARIGAFIVTGLLLLGGGTLYAREYATRSEEPSAER MAV_4116|M.avium_104 KPLVDSQFAPV---QVATVVAAAIGVGAAGAAPLLARHRERTAKSE---- MLBr_02582|M.leprae_Br4923 GSNAKTAPSPYRGLLIAAHLTWLVILVMAVVGMQITRHTHFRDVSSVALL MUL_1004|M.ulcerans_Agy99 GIADTGSGS--------IMSTIFGQVTTKERQCQFMPTIGSTVVP----- . : Mb0298|M.bovis_AF2122/97 NR------------- Rv0290|M.tuberculosis_H37Rv NR------------- Mflv_3578|M.gilvum_PYR-GCK FDTT----------- Mvan_2837|M.vanbaalenii_PYR-1 --------------- TH_4594|M.thermoresistible__bu ESRGTGKHQADNEPV MMAR_3960|M.marinum_M --------------- MSMEG_3241|M.smegmatis_MC2_155 PGTDHEAHST----- MAB_4509c|M.abscessus_ATCC_199 PVT------------ MAV_4116|M.avium_104 --------------- MLBr_02582|M.leprae_Br4923 SRR------------ MUL_1004|M.ulcerans_Agy99 ---------------