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AFFKKPDVGSWTENWPELGTAPVNYEDSIDPEQWKLEQQAIFRKTWLQMGRIELIPKKGSYITRELPSVG PGTSIVIVNDGEQIRAFYNLCRHRGNKLVWNDYPGEETSGTCRQFVCKYHAWRYDLKGDLKFVQQEQEFF DLDKQDYPLKPVRCEVWEGFIFVNFDDDAVSLEEYLGEFGQGLKGYPFHEMTEHYSYRAEIKSNWKLFID AFVEFYHAPILHMKQAEKEEAEKLAKFGFEALHYDIKGDHSMISSWGGMSPPKDLNMVKPIERILHSGLF GPWDRPDIKGILPDELPPAINPGRHKTWGQDSFEFFPNFTLLFWVPGWYLTYNYWPTGVDTHIFEADLYF VPPKNLRERLSQELAAVTFKEYAFQDANTLEATQTQIGTRAVLDFPLCDQEVLLRHLHHTAHKYVDRYKA ELEAKSNGSSNGATTSSTTATQKDEVNA*
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. gilvum PYR-GCK | Mflv_2439 | - | - | 100% (449) | Rieske (2Fe-2S) domain-containing protein |
| M. gilvum PYR-GCK | Mflv_2464 | - | e-148 | 56.74% (430) | Rieske (2Fe-2S) domain-containing protein |
| M. gilvum PYR-GCK | Mflv_2470 | - | e-117 | 48.59% (426) | Rieske (2Fe-2S) domain-containing protein |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb3186c | - | 6e-24 | 28.70% (223) | dioxygenase |
| M. tuberculosis H37Rv | Rv3161c | - | 5e-24 | 28.70% (223) | dioxygenase |
| M. leprae Br4923 | - | - | - | - | - |
| M. abscessus ATCC 19977 | MAB_4370c | - | 2e-17 | 29.00% (200) | putative large terminal subunit of phenylpropionate dioxygenase |
| M. marinum M | MMAR_4744 | - | 1e-180 | 68.63% (424) | dioxygenase |
| M. avium 104 | MAV_0905 | - | 1e-178 | 67.22% (421) | Rieske (2Fe-2S) domain-containing protein |
| M. smegmatis MC2 155 | MSMEG_4840 | - | 0.0 | 84.09% (440) | Rieske (2Fe-2S) domain-containing protein |
| M. thermoresistible (build 8) | TH_3820 | - | 0.0 | 79.46% (443) | PUTATIVE Rieske [2Fe-2S] domain protein |
| M. ulcerans Agy99 | MUL_4884 | - | 6e-11 | 30.46% (174) | dioxygenase |
| M. vanbaalenii PYR-1 | Mvan_4213 | - | 0.0 | 80.40% (449) | Rieske (2Fe-2S) domain-containing protein |
CLUSTAL 2.0.9 multiple sequence alignment
Mb3186c|M.bovis_AF2122/97 --MLSTDNRAELGDILTDIGDYLDDNPPALSLPP---AAYTS---SELWQ
Rv3161c|M.tuberculosis_H37Rv --MLSTDNRAELGDILTDIGDYLDDNPPALSLPP---AAYTS---SELWQ
MSMEG_4840|M.smegmatis_MC2_155 -----------MAHFPKPAVGSWTENWPELGTAP---VDYTDSIDPEQWK
Mvan_4213|M.vanbaalenii_PYR-1 -----------MAHFPKPAAGSWTENWPELGTAP---VDYTDSIDPEQWK
TH_3820|M.thermoresistible__bu -----------VAHFPKPAVGSWTENWPELGTAP---VDYTDSIDPEQWK
Mflv_2439|M.gilvum_PYR-GCK -----------MAFFKKPDVGSWTENWPELGTAP---VNYEDSIDPEQWK
MMAR_4744|M.marinum_M -----------MPHFTKPAAGSWTQNYPELGTAP---VDYTDSIDPQYFA
MAV_0905|M.avium_104 -----------MPHFPKPAAGSWTENYPELGTGP---VDYTDSIDPAFFE
MUL_4884|M.ulcerans_Agy99 MSSPTKPLVAEDAEIVRRVLAHIDAGTTDEGEAW---REPVENYASPERF
MAB_4370c|M.abscessus_ATCC_199 MASLPRPVAVAQGALCRPQALVRDIDVSGLVDSHGGLLDRVIFTDGELYR
: . .
Mb3186c|M.bovis_AF2122/97 LERERIFNRSWMLVAHVDQLAKTGDYVTVSV----AGEPVMVVRDVDGQL
Rv3161c|M.tuberculosis_H37Rv LERERIFNRSWMLVAHVDQVAKTGDYVTVSV----AGEPVMVVRDVDGQL
MSMEG_4840|M.smegmatis_MC2_155 LEQQAIFRRTWLHVGRVERLPKTGSYFTREMPSVGKGTSIIVVKDKDGAV
Mvan_4213|M.vanbaalenii_PYR-1 LEQQAIFRKLWLHVGRVERLPKAGSYFTREMPSVGAGTSIIVNKDKDGQV
TH_3820|M.thermoresistible__bu LEQQAIFRRLWLHVGRVERLPKAGSYFTREMPSVGAGTSIIVNRDKDGTI
Mflv_2439|M.gilvum_PYR-GCK LEQQAIFRKTWLQMGRIELIPKKGSYITRELPSVGPGTSIVIVNDGE-QI
MMAR_4744|M.marinum_M DEQRAIFRRTWLNVGRIERLPRTGSYFTKELPSTGAGMSVIVVKGKDGVI
MAV_0905|M.avium_104 AEREAIFKKTWLNVGRVNRLPRTGSYFTRELPSAGKGTSVIIVKTKDGSV
MUL_4884|M.ulcerans_Agy99 AAELRLLRGMPSVFVPSAAIPNNGDHVERTA----FGVPLFAVRGADGRA
MAB_4370c|M.abscessus_ATCC_199 QELRRVFAPSWLFLAHADQFHRPGDFFTTYMG----EDPVIVSMDKNRRI
:: . . . *... .:. :
Mb3186c|M.bovis_AF2122/97 HALSPICRHR---LMLMVEPG---AGRIDTLTCQYHLWRYGLDGRLRGAP
Rv3161c|M.tuberculosis_H37Rv HALSPICRHR---LMLMVEPG---AGRIDTLTCQYHLWRYGLDGRLRGAP
MSMEG_4840|M.smegmatis_MC2_155 RAFYNMCRHRGNKLVWNDYPGEEVSGTCRQFTCKYHAWRYALNGDLTFIQ
Mvan_4213|M.vanbaalenii_PYR-1 RAFYNLCRHRGNKLVWNDYPGEEVSGSCRQFTCKYHAWRYALDGELTFIQ
TH_3820|M.thermoresistible__bu RAFYNLCRHRGNKLVWNDYPGEEVSGTCRQFTCKYHGWRYALNGDLTFIQ
Mflv_2439|M.gilvum_PYR-GCK RAFYNLCRHRGNKLVWNDYPGEETSGTCRQFVCKYHAWRYDLKGDLKFVQ
MMAR_4744|M.marinum_M RAFHNICRHRGNKLVWNDFPHEETAGTCRQFTCKYHAWRYGLDGELTFIQ
MAV_0905|M.avium_104 KAYHNVCRHRGNKLVWNDFPNEETSGTCRQFTCKYHAWRYSLDGDLTFVQ
MUL_4884|M.ulcerans_Agy99 RVFRNARRHRGFALVEN-------AGCSHALVCRYHGWIYRLDGSLSHVP
MAB_4370c|M.abscessus_ATCC_199 RAFLNSCRHRGARVCRADY------GVTRGFTCTYHGWAYDLEGKLISVP
:. *** : * :.* ** * * *.* *
Mb3186c|M.bovis_AF2122/97 HMAANLD-FNRRECRLPQFAVATWNGLVWIN-LDADAEPIAAHLDLTDDE
Rv3161c|M.tuberculosis_H37Rv HMAANLD-FNRRECRLPQFAVATWNGLVWIN-LDADAEPIAAHLDLTDDE
MSMEG_4840|M.smegmatis_MC2_155 QEDEFFD-VDKADYPLKPVRCEIWEGFIFVN-FDDDAMPLEEYLGEFGQG
Mvan_4213|M.vanbaalenii_PYR-1 QEDEFFD-VDKADYPLKPVRCEVWEGFIFVN-FDDDAEPLVDYLGDFAKG
TH_3820|M.thermoresistible__bu QEDEFFD-VDKADYGLKPIRCEVWEGFIFVN-FDDDAEPLEDYLGEFGQN
Mflv_2439|M.gilvum_PYR-GCK QEQEFFD-LDKQDYPLKPVRCEVWEGFIFVN-FDDDAVSLEEYLGEFGQG
MMAR_4744|M.marinum_M QEDEFFD-VDKSQYGLKSVRCEVWEGFIFIN-LDPAAAPLSEYLGSMAKG
MAV_0905|M.avium_104 QEEEFFD-LDKSNYGLAPVRCEVWEGFIFIN-FDDNAAPLTDYLGPLAKS
MUL_4884|M.ulcerans_Agy99 HAEAFPD-LDMSARGLVEVESHEVDGLIVIGPLDPVDAGLIEDEGAIGWL
MAB_4370c|M.abscessus_ATCC_199 NEEGYPDSFSTDDWGLVEVPHVDSYHGLIFGTWNPAPVGLRESLGDMAWY
: * .. * . : .. : : .
Mb3186c|M.bovis_AF2122/97 FAGYRLGEMVQVESWSHEWRA-----NWKVAAENGHENYHVLGLHRQTLE
Rv3161c|M.tuberculosis_H37Rv FAGYRLGEMVQVESWSHEWRA-----NWKVAAENGHENYHVLGLHRQTLE
MSMEG_4840|M.smegmatis_MC2_155 LKGYPFHEMTEVYSYRAEINS-----NWKLFIDAFVEFYHAPILHMKQAT
Mvan_4213|M.vanbaalenii_PYR-1 LEGYPFHEMTEVYSYRAEINS-----NWKLFIDAFVEFYHAPILHMKQAT
TH_3820|M.thermoresistible__bu LKGYPFHEMTEVYSYRAEVNA-----NWKLFIDAFTEFYHAPVLHNKQAV
Mflv_2439|M.gilvum_PYR-GCK LKGYPFHEMTEHYSYRAEIKS-----NWKLFIDAFVEFYHAPILHMKQAE
MMAR_4744|M.marinum_M LQGYPFHEMTEVYSYRAEVGS-----NWKLFIDAFAEFYHAPVLHQGQYT
MAV_0905|M.avium_104 IEGYPFGEMTETYSYRAEVGS-----NWKLFIDAFVEFYHAPILHQGQYT
MUL_4884|M.ulcerans_Agy99 TDGSPWRAKLVPAERLIVVESTLREINWKVLVEQFLEGYHIRSTHKDTFF
MAB_4370c|M.abscessus_ATCC_199 MDAMLDHDSEGTVVVGGVHKWVLDG-NWKLAAEQFATDWYHVNMSHASAL
. ***: : ::
Mb3186c|M.bovis_AF2122/97 P---------------FVPGGGDLDVRQYSRWALRLRVPFTVPVEAKS--
Rv3161c|M.tuberculosis_H37Rv P---------------FVPGGGDLDVRQYSRWALRLRVPFTVPVEAKS--
MSMEG_4840|M.smegmatis_MC2_155 PEEAAKLAKIGFEALHYDIKGDHSMISSWGGMSPPKDLNMVKPIERILHS
Mvan_4213|M.vanbaalenii_PYR-1 AEEAAKLAKVGFEALHYDIKDQHSMISSWGGMSPPKDLNMVKPIERILHS
TH_3820|M.thermoresistible__bu KEEAEKLAKYGYEALHYDIKGPHSMISSWGGMAPPKDPSMVKPIERILNS
Mflv_2439|M.gilvum_PYR-GCK KEEAEKLAKFGFEALHYDIKGDHSMISSWGGMSPPKDLNMVKPIERILHS
MMAR_4744|M.marinum_M KEEAAKIMKYGFEALHYELAGPHAMISVWGGQSPPADLKMVKPMDRVLRS
MAV_0905|M.avium_104 KEEAAKIQKFGYEALHYELAGPHSLQSTWGGQAPPPDMSMVKPMDQVLRS
MUL_4884|M.ulcerans_Agy99 P-------------------VQYDDLNVVEAFGPNTRITFPYRNIERLR-
MAB_4370c|M.abscessus_ATCC_199 MVMAPQGKAPKDEIVHRTGRQYVDPNGHGAGFPVHPKNRFDARVVHQWTD
:
Mb3186c|M.bovis_AF2122/97 ----------------LQLNEVQKSNLVVLWT------FPNSALAIAG-E
Rv3161c|M.tuberculosis_H37Rv ----------------LQLNEVQKSNLVVLWT------FPNSALAIAG-E
MSMEG_4840|M.smegmatis_MC2_155 GLFGPWDRPDIKGILPDELPPAVNPARQPTWGQDSFEFFPNFTLLLWVPG
Mvan_4213|M.vanbaalenii_PYR-1 GLFGPWDRPDIKGILPDELPPAVNPARQPTWGQDSFEFFPNFTLLLWAPG
TH_3820|M.thermoresistible__bu GLFGPWDRPDIKGIAPDELPPAINPSRQPFWGQDSFEFFPNFTLLIWAPG
Mflv_2439|M.gilvum_PYR-GCK GLFGPWDRPDIKGILPDELPPAINPGRHKTWGQDSFEFFPNFTLLFWVPG
MMAR_4744|M.marinum_M GLFGPWDRPAP-ITDLEELPPGINPTKAKQWGIDSFLFFPNFMLLIWEPG
MAV_0905|M.avium_104 GLFGPWDKPE--IIEKLDLPPGVNVKRVPQWGIDSWLFYPNFMLLIWEPG
MUL_4884|M.ulcerans_Agy99 -------------------DRPEHTWTTGARVTYLYQLFPNVMLATFP-D
MAB_4370c|M.abscessus_ATCC_199 YG--------------ALRERLGEARVQGPLTNGHATVFPNLSYLPVNGS
. :**
Mb3186c|M.bovis_AF2122/97 RVVWFGFIPQSIDRVQVLGGVLTTPELAADAAATAQTSQFVMAMIN----
Rv3161c|M.tuberculosis_H37Rv RVVWFGFIPQSIDRVQVLGGVLTTPELAADAAATAQTSQFVMAMIN----
MSMEG_4840|M.smegmatis_MC2_155 WYLTYNYWPTDVDKHIFECNLYFVPPKNTRQRLAQELAAVTFKEYA----
Mvan_4213|M.vanbaalenii_PYR-1 WYLTYNYWPTAVDKHIFECNLYFVPPKNTRQRLSQELAAVTFKEYA----
TH_3820|M.thermoresistible__bu WYLTYNYWPTDVTKHIFEASLYFVPPKTTRQRLAQELAAVTFKEYA----
Mflv_2439|M.gilvum_PYR-GCK WYLTYNYWPTGVDTHIFEADLYFVPPKNLRERLSQELAAVTFKEYA----
MMAR_4744|M.marinum_M WYLTYHYWPTTVDKHIFETTLYFVPPRNARERLAQELAAVTFKEYA----
MAV_0905|M.avium_104 WFLTYHYWPTAVDRHIFESTLYFVPPRNARERLAQELAAVTFKEYA----
MUL_4884|M.ulcerans_Agy99 VIAMVVIDPVSVDRTAVTIYSMVTPDVAERDSLDASSNLAGAGSFIGQGL
MAB_4370c|M.abscessus_ATCC_199 VRIWHPKGPDKMEVWAWTIVDRSMPEEVRDAQRLYNLRTFGPTGIFEQDD
* : * .
Mb3186c|M.bovis_AF2122/97 DEDRLGLEAVQVGAGSRFAERGHLSSKEWPGMLAFYRNLAMALVGDHP--
Rv3161c|M.tuberculosis_H37Rv DEDRLGLEAVQVGAGSRFAERGHLSSKEWPGMLAFYRNLAMALVGDHP--
MSMEG_4840|M.smegmatis_MC2_155 LQDANTLEATQTQINTRAVTEFPLCDQEV--LLRHLHHTAHKYVDAYKAE
Mvan_4213|M.vanbaalenii_PYR-1 LQDANTLEATQTQIGTRAVTEFPLCDQEI--LLRHLHHTAHKYVDKYKLE
TH_3820|M.thermoresistible__bu LQDANTLEATQTQIGTRAVTEFPLCDQEV--LLRHLHHTAHQYVDKYKEE
Mflv_2439|M.gilvum_PYR-GCK FQDANTLEATQTQIGTRAVLDFPLCDQEV--LLRHLHHTAHKYVDRYKAE
MMAR_4744|M.marinum_M LQDSNTLEATQTMIGTGVVKDFPLCDQEI--LLRHLHKMTGDYVKEYR--
MAV_0905|M.avium_104 LQDANTLEATQTMIGTRAVKEFLLCDQEV--LIRHLHKTTGDYVKEYQ--
MUL_4884|M.ulcerans_Agy99 FEDNEMSEGVQRGLAAGANAFVEFGRHES--AVGRFHAALGRRLARLT--
MAB_4370c|M.abscessus_ATCC_199 GENWSEVQSISRGFVTNSVALNYQMGLDSEREDGRHPGLTSELYSDAAGR
:: :. . : :
Mb3186c|M.bovis_AF2122/97 --------------GAS----------
Rv3161c|M.tuberculosis_H37Rv --------------GAS----------
MSMEG_4840|M.smegmatis_MC2_155 LAEKTQ---KAESNGSAVTSEKDAAHV
Mvan_4213|M.vanbaalenii_PYR-1 QAAK------AATNGSASKTEKEGANV
TH_3820|M.thermoresistible__bu LAQK-----AQQTNGSKKEGARSTAHV
Mflv_2439|M.gilvum_PYR-GCK LEAKSNGSSNGATTSSTTATQKDEVNA
MMAR_4744|M.marinum_M ------------NNGHAK---------
MAV_0905|M.avium_104 ------------NNGHVAAR-------
MUL_4884|M.ulcerans_Agy99 --------------SAG----------
MAB_4370c|M.abscessus_ATCC_199 SFYAR-------WRDLMNTKAWHEEHV
.