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MPKWPKPAEGSWTEHYPELGTGPVSFRDSTSPEFYELEREAVFKRAWLNVGRVEELPRVGSYLTKDIDAA RTSVIVVKGRDGQIRAFHNVCRHRGNKLVWNDFPSEEVKGACRQFTCKYHGWRYGLDGELTFVQQPGEFF GLDTAKYGLKSVHCDVWNGFIFINFDAEPRQTLREFLGPMITALDDYPFELMTERYDFVAHNNSNWKIFA DAFQEYYHVPSLHSQQVPTEVRQPNATFECGHFQIDGPHRLVSTAGTRRWLLAPEYMYPVERATHSGLVG PWRTPETHQSAGLNPGGIEPWGITNFQIFPNLEILIYHGWYLLYRYWPTSHNTHKFEAYNAFHPARTVRE RVEHEVASVVLKEFALQDAGMLGGTQAALEYGLGEPIVDDYPLNDQEILVRHLHHVAVDWVDRYKAERDA QRAERDPVGV
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. gilvum PYR-GCK | Mflv_2470 | - | - | 100% (430) | Rieske (2Fe-2S) domain-containing protein |
| M. gilvum PYR-GCK | Mflv_2464 | - | e-152 | 58.75% (417) | Rieske (2Fe-2S) domain-containing protein |
| M. gilvum PYR-GCK | Mflv_2439 | - | e-117 | 48.59% (426) | Rieske (2Fe-2S) domain-containing protein |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb3186c | - | 4e-25 | 32.86% (213) | dioxygenase |
| M. tuberculosis H37Rv | Rv3161c | - | 9e-25 | 32.39% (213) | dioxygenase |
| M. leprae Br4923 | - | - | - | - | - |
| M. abscessus ATCC 19977 | MAB_4370c | - | 2e-19 | 32.87% (216) | putative large terminal subunit of phenylpropionate dioxygenase |
| M. marinum M | MMAR_4744 | - | 1e-125 | 51.53% (425) | dioxygenase |
| M. avium 104 | MAV_3961 | - | 0.0 | 80.95% (420) | Rieske (2Fe-2S) domain-containing protein |
| M. smegmatis MC2 155 | MSMEG_0293 | - | 0.0 | 74.52% (416) | Rieske (2Fe-2S) domain-containing protein |
| M. thermoresistible (build 8) | TH_3877 | - | 0.0 | 87.86% (420) | PUTATIVE Rieske (2Fe-2S) domain protein |
| M. ulcerans Agy99 | MUL_3820 | - | 9e-20 | 28.94% (235) | ring-hydroxylating dioxygenase, large terminal subunit |
| M. vanbaalenii PYR-1 | Mvan_4184 | - | 0.0 | 88.84% (430) | Rieske (2Fe-2S) domain-containing protein |
CLUSTAL 2.0.9 multiple sequence alignment
Mb3186c|M.bovis_AF2122/97 -----MLSTDNRAELGDILTDIGDYLDDNPPALSLPPAAY---TSSELWQ
Rv3161c|M.tuberculosis_H37Rv -----MLSTDNRAELGDILTDIGDYLDDNPPALSLPPAAY---TSSELWQ
Mflv_2470|M.gilvum_PYR-GCK --------------MPKWPKPAEGSWTEHYPELGTGPVSFRDSTSPEFYE
Mvan_4184|M.vanbaalenii_PYR-1 --------------MAKWPKPVEGSWTQHYPDLGTGPVSFRDSTSPEFYE
TH_3877|M.thermoresistible__bu --------------MKKWPKPAEGSWTEHYPELGTGPVSFRDSTSPEFYE
MAV_3961|M.avium_104 --------------MTRSGRPPEGSWTEHYPELGTGPVSFKDSTSPEFYE
MSMEG_0293|M.smegmatis_MC2_155 -------------MATKWPKPAEGSWTEHYPELGTGPISFRDSTSREFYA
MMAR_4744|M.marinum_M --------------MPHFTKPAAGSWTQNYPELGTAPVDYTDSIDPQYFA
MUL_3820|M.ulcerans_Agy99 ---------MDRDQLIDLTRRALKLARDKTTDLAPHQHAVDAREYTSLDR
MAB_4370c|M.abscessus_ATCC_199 MASLPRPVAVAQGALCRPQALVRDIDVSGLVDSHGGLLDRVIFTDGELYR
. .
Mb3186c|M.bovis_AF2122/97 LERE-RIFNRSWMLVAHVDQLAKTGDYVTVSVAGEPV----MVVRDVDGQ
Rv3161c|M.tuberculosis_H37Rv LERE-RIFNRSWMLVAHVDQVAKTGDYVTVSVAGEPV----MVVRDVDGQ
Mflv_2470|M.gilvum_PYR-GCK LERE-AVFKRAWLNVGRVEELPRVGSYLTKDIDAART--SVIVVKGRDGQ
Mvan_4184|M.vanbaalenii_PYR-1 LERE-AIFKRAWLNVARVEELPRAGSYLTKEIEVAKT--SVIVVKGRDEQ
TH_3877|M.thermoresistible__bu LERE-AVFKRAWLHVGRVEELPRAGSYLTKNIDVAKT--SVIVVRGRDEQ
MAV_3961|M.avium_104 LERE-AIFKRAWLNVARVEELPRVGSYLTKEIEAART--SVIVVKGRDER
MSMEG_0293|M.smegmatis_MC2_155 LERE-AIFKRAWLNISRVEETPDAGSYITRRVEAADA--SLLLVKGADGR
MMAR_4744|M.marinum_M DEQR-AIFRRTWLNVGRIERLPRTGSYFTKELPSTGAGMSVIVVKGKDGV
MUL_3820|M.ulcerans_Agy99 HERDRAMVLDSPQLVGYSSELPGPDTYRTKTIMGRSI----LLTRAADRA
MAB_4370c|M.abscessus_ATCC_199 QELR-RVFAPSWLFLAHADQFHRPGDFFTTYMGEDPV----IVSMDKNRR
* :. : :. .. . : * : :: :
Mb3186c|M.bovis_AF2122/97 LHALSPICRHR------LMLMVEPGAGRIDTLTCQYHLWRYGLDGRLRGA
Rv3161c|M.tuberculosis_H37Rv LHALSPICRHR------LMLMVEPGAGRIDTLTCQYHLWRYGLDGRLRGA
Mflv_2470|M.gilvum_PYR-GCK IRAFHNVCRHRGNKLVWNDFPSEEVKGACRQFTCKYHGWRYGLDGELTFV
Mvan_4184|M.vanbaalenii_PYR-1 IRAFHNICRHRGNKLVWNDYPNEEVKGTCRQFTCKYHGWRYNLTGDLTFV
TH_3877|M.thermoresistible__bu IRAFYNICRHRGNKLVWNDYPHEEVKGACRQFTCKYHGWRYDLKGDLTFV
MAV_3961|M.avium_104 IRAFYNVCRHRGNKLVWNDFPGEETRGTCRQFTCKYHGWRYDLTGALKFV
MSMEG_0293|M.smegmatis_MC2_155 IRAFHNVCRRRGHTVAAPDFRATELRNTGTEFACGHCGWRYGLDGSLIAA
MMAR_4744|M.marinum_M IRAFHNICRHRGNKLVWNDFPHEETAGTCRQFTCKYHAWRYGLDGELTFI
MUL_3820|M.ulcerans_Agy99 VRAFDNVCLHR-------QSRVATGCGTALRFTCPYHGWTYDNGGRLVGL
MAB_4370c|M.abscessus_ATCC_199 IRAFLNSCRHRG------ARVCRADYGVTRGFTCTYHGWAYDLEGKLISV
::*: * :* . ::* : * *. * *
Mb3186c|M.bovis_AF2122/97 PHMAANLD-FNRRECRLPQF-AVATWNGLVWINLDA----DAEPIAAHLD
Rv3161c|M.tuberculosis_H37Rv PHMAANLD-FNRRECRLPQF-AVATWNGLVWINLDA----DAEPIAAHLD
Mflv_2470|M.gilvum_PYR-GCK QQPGEFFG-LDTAKYGLKSV-HCDVWNGFIFINFDAE---PRQTLREFLG
Mvan_4184|M.vanbaalenii_PYR-1 QQPGEFFG-LDEKDYGLAPV-HCDVWNGFVFINFDRE---PRQTLREFLG
TH_3877|M.thermoresistible__bu QQESEFFD-LDTARYGLKPV-NCDVWNGFIFINFDPE---PKWSLREFLG
MAV_3961|M.avium_104 QQESEFFD-LDPAEYGLRPV-HCDVWNGFVFINFDPQ---PRQSLREFLG
MSMEG_0293|M.smegmatis_MC2_155 PDADRFTG-FDATELGLAEV-HCDVWAGFVFVNFDTT---PRQSLREFLG
MMAR_4744|M.marinum_M QQEDEFFD-VDKSQYGLKSV-RCEVWEGFIFINLDP----AAAPLSEYLG
MUL_3820|M.ulcerans_Agy99 PGREGFPG-IAVGSDGLTEL-PATEFAGFLWVSLHPA---ARLDVPAHLG
MAB_4370c|M.abscessus_ATCC_199 PNEEGYPDSFSTDDWGLVEVPHVDSYHGLIFGTWNPAPVGLRESLGDMAW
. . * . : *::: . . :
Mb3186c|M.bovis_AF2122/97 LTDDEFAGYRLGEMVQVESWSHEWRANWKVAAENGH-ENYHVLGLHRQTL
Rv3161c|M.tuberculosis_H37Rv LTDDEFAGYRLGEMVQVESWSHEWRANWKVAAENGH-ENYHVLGLHRQTL
Mflv_2470|M.gilvum_PYR-GCK PMITALDDYPFELMTERYDFVAHNNSNWKIFADAFQ-EYYHVPSLHSQQV
Mvan_4184|M.vanbaalenii_PYR-1 PMITALDDYPFESMTERYDFVAHNNSNWKIFADAFQ-EYYHVPSLHSQQV
TH_3877|M.thermoresistible__bu PMITALDDYPFGLMTERYEFEAHNNSNWKIFADAFQ-EYYHVPSLHSQQV
MAV_3961|M.avium_104 PMITGLDGYPFDKLTERYDWVAHNNSNWKIFADAFQ-EYYHVPALHSQQV
MSMEG_0293|M.smegmatis_MC2_155 PMVTGLDDYPFEKLTERYDWVAHNNSNWKIFADAFQ-EYYHVPSLHTQQV
MMAR_4744|M.marinum_M SMAKGLQGYPFHEMTEVYSYRAEVGSNWKLFIDAFA-EFYHAPVLHQGQY
MUL_3820|M.ulcerans_Agy99 PLAGELQSWGIGRWSPLGEKVLGSPIDWKLATDTFA-ENYHFATVHRRTF
MAB_4370c|M.abscessus_ATCC_199 YMDAMLDHDSEGTVVVGGVHKWVLDGNWKLAAEQFATDWYHVNMSHASAL
: :**: : : ** *
Mb3186c|M.bovis_AF2122/97 EP---------------FVPGGGDLDVRQYSRWALRLRVPFTVPVEAKS-
Rv3161c|M.tuberculosis_H37Rv EP---------------FVPGGGDLDVRQYSRWALRLRVPFTVPVEAKS-
Mflv_2470|M.gilvum_PYR-GCK P--TEVRQPNATFECGHFQIDGPHRLVSTAGTRRWLLAPEYMYPVERATH
Mvan_4184|M.vanbaalenii_PYR-1 P--SAVRQPNATFECGHFQIDGPHRLVSTAGTRRWLLAPEYMYPVERVTR
TH_3877|M.thermoresistible__bu P--SAVRQPNATFECGHFQIDGPHRLVSTAGTRRWLMPPEYMYPVERATR
MAV_3961|M.avium_104 P--PEVRDSNAVFTCGHFQLDGPHRLVSTAGRRRWLMPPEYMYPIERATR
MSMEG_0293|M.smegmatis_MC2_155 P--PEVRDPNAGFTCGHFQIDGPHRLVSTAGTRRWLLAPEYMYPIERATQ
MMAR_4744|M.marinum_M TKEEAAKIMKYGFEALHYELAGPHAMISVWGGQSPPADLKMVKPMDRVLR
MUL_3820|M.ulcerans_Agy99 AT---------------IARSNCTVFDSYGPHHRLIFPLNTILELENVPE
MAB_4370c|M.abscessus_ATCC_199 MV---MAPQGKAPKDEIVHRTGRQYVDPNGHGAGFPVHPKNRFDARVVHQ
.
Mb3186c|M.bovis_AF2122/97 ----------------------LQLNEVQKSNLVVLWTFPNSALAIAG-E
Rv3161c|M.tuberculosis_H37Rv ----------------------LQLNEVQKSNLVVLWTFPNSALAIAG-E
Mflv_2470|M.gilvum_PYR-GCK SGLVGPWRTPET-----HQSAGLNPGGIEPWGITNFQIFPNLEILIYH-G
Mvan_4184|M.vanbaalenii_PYR-1 SGLVGPWRTPET-----HQSAGLNPGGIEPWGITNFQIFPNLEILIYH-G
TH_3877|M.thermoresistible__bu SGLVGPWRTPKT-----HFSAGLNPGGIEPWGITNFQIFPNLEILIYH-G
MAV_3961|M.avium_104 SGLVGPWRTPDIG----ELPAGLNPGRIEQWGISNFQIFPNLEILIYG-G
MSMEG_0293|M.smegmatis_MC2_155 SGLVGPWRTPDIG----KLPTGLNPGGIEPWGISNFQIFPNIEILIYG-G
MMAR_4744|M.marinum_M SGLFGPWDRPAPITDLEELPPGINPTKAKQWGIDSFLFFPNFMLLIWEPG
MUL_3820|M.ulcerans_Agy99 -----------------------GQWDPFHHMVVIYALFPNVVLSVTI-A
MAB_4370c|M.abscessus_ATCC_199 WTDYGALRERLG-----------EARVQGPLTNGHATVFPNLSYLPVNGS
***
Mb3186c|M.bovis_AF2122/97 RVVWFGFIPQSIDRVQVLGGVLTTPELAADAAATAQTSQFVMAMIN---D
Rv3161c|M.tuberculosis_H37Rv RVVWFGFIPQSIDRVQVLGGVLTTPELAADAAATAQTSQFVMAMIN---D
Mflv_2470|M.gilvum_PYR-GCK WYLLYRYWPTSHNTHKFEAYNAFHPARTVRERVEHEVASVVLKEFA---L
Mvan_4184|M.vanbaalenii_PYR-1 WYLLYRYWPTSHSTHKFEAYNAFHPARTVRERIEHEVASVVLKEFA---L
TH_3877|M.thermoresistible__bu WYLLYQYWPTSYNTHRFEAYTAFHPARTVRERIEHEVASVVLKEFA---L
MAV_3961|M.avium_104 WYLLYRYWPTSHNTHRFEAYTYFHPARSVRERIEHEVAAVVLKEFA---L
MSMEG_0293|M.smegmatis_MC2_155 WYLLYRYWPTSHNTHRYEAFTYFHPARTVRERIEHEVAAVVLKEFA---L
MMAR_4744|M.marinum_M WYLTYHYWPTTVDKHIFETTLYFVPPRNARERLAQELAAVTFKEYA---L
MUL_3820|M.ulcerans_Agy99 NGELFRIYPGDRPGSSITVHQNSTPLDLSDESVATGAQAVFDYAHATVRD
MAB_4370c|M.abscessus_ATCC_199 VRIWHPKGPDKMEVWAWTIVDRSMPEEVRDAQRLYNLRTFGPTGIFE--Q
. * * .
Mb3186c|M.bovis_AF2122/97 EDR--LGLEAVQVGAGSRFAERGHLSSKEWPGMLAFYRNLAMALVGDHPG
Rv3161c|M.tuberculosis_H37Rv EDR--LGLEAVQVGAGSRFAERGHLSSKEWPGMLAFYRNLAMALVGDHPG
Mflv_2470|M.gilvum_PYR-GCK QDAGMLGGTQAALEYGLGEPIVDDYPLNDQEILVRHLHHVAVDWVDRYKA
Mvan_4184|M.vanbaalenii_PYR-1 QDAGMLGGTQAALEYGLDEPIVDDYPLNDQEILVRHLHKTAVDWVEEYQN
TH_3877|M.thermoresistible__bu QDAGMLGGTQAALEYGLDEPIVDEFPLSDQEILVRHLHKVVVDWVEEYQS
MAV_3961|M.avium_104 QDAGMLGGTQAALEYG----VVEDFPLNDQEILVRHLHKVVVDWVDAYRR
MSMEG_0293|M.smegmatis_MC2_155 QDAGMLGGTQAALEYD----VIDDYPLNDQEILVRHLHKVAVDWVEAYQR
MMAR_4744|M.marinum_M QDSNTLEATQTMIGTG----VVKDFPLCDQEILLRHLHKMTGDYVKEYRN
MUL_3820|M.ulcerans_Agy99 EDYRLAETLQTNLESG----ARQRLVFGRNEPGLQHRHITWAQALETPG-
MAB_4370c|M.abscessus_ATCC_199 DDGENWSEVQSISRGFVTNSVALNYQMGLDSEREDGRHPGLTSELYSDAA
:* : :
Mb3186c|M.bovis_AF2122/97 AS--------------------
Rv3161c|M.tuberculosis_H37Rv AS--------------------
Mflv_2470|M.gilvum_PYR-GCK ERDAQRAERDPVGV--------
Mvan_4184|M.vanbaalenii_PYR-1 E-------RRPVGV--------
TH_3877|M.thermoresistible__bu E-------RTAVGV--------
MAV_3961|M.avium_104 E-------RETVGV--------
MSMEG_0293|M.smegmatis_MC2_155 E-------PAPAGV--------
MMAR_4744|M.marinum_M N--------GHAK---------
MUL_3820|M.ulcerans_Agy99 ----------------------
MAB_4370c|M.abscessus_ATCC_199 GRSFYARWRDLMNTKAWHEEHV