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PHFPKPAAGSWTENYPELGTGPVDYTDSIDPAFFEAEREAIFKKTWLNVGRVNRLPRTGSYFTRELPSAG KGTSVIIVKTKDGSVKAYHNVCRHRGNKLVWNDFPNEETSGTCRQFTCKYHAWRYSLDGDLTFVQQEEEF FDLDKSNYGLAPVRCEVWEGFIFINFDDNAAPLTDYLGPLAKSIEGYPFGEMTETYSYRAEVGSNWKLFI DAFVEFYHAPILHQGQYTKEEAAKIQKFGYEALHYELAGPHSLQSTWGGQAPPPDMSMVKPMDQVLRSGL FGPWDKPEIIEKLDLPPGVNVKRVPQWGIDSWLFYPNFMLLIWEPGWFLTYHYWPTAVDRHIFESTLYFV PPRNARERLAQELAAVTFKEYALQDANTLEATQTMIGTRAVKEFLLCDQEVLIRHLHKTTGDYVKEYQNN GHVAAR*
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. avium 104 | MAV_0905 | - | - | 100% (427) | Rieske (2Fe-2S) domain-containing protein |
| M. avium 104 | MAV_3967 | - | e-155 | 59.95% (417) | Rieske (2Fe-2S) domain-containing protein |
| M. avium 104 | MAV_3961 | - | e-129 | 53.01% (415) | Rieske (2Fe-2S) domain-containing protein |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb3186c | - | 4e-27 | 32.55% (212) | dioxygenase |
| M. gilvum PYR-GCK | Mflv_2439 | - | 1e-178 | 67.22% (421) | Rieske (2Fe-2S) domain-containing protein |
| M. tuberculosis H37Rv | Rv3161c | - | 1e-26 | 32.08% (212) | dioxygenase |
| M. leprae Br4923 | - | - | - | - | - |
| M. abscessus ATCC 19977 | MAB_4370c | - | 2e-16 | 26.87% (227) | putative large terminal subunit of phenylpropionate dioxygenase |
| M. marinum M | MMAR_4744 | - | 0.0 | 79.01% (424) | dioxygenase |
| M. smegmatis MC2 155 | MSMEG_4840 | - | 0.0 | 71.26% (421) | Rieske (2Fe-2S) domain-containing protein |
| M. thermoresistible (build 8) | TH_3820 | - | 0.0 | 70.07% (421) | PUTATIVE Rieske [2Fe-2S] domain protein |
| M. ulcerans Agy99 | MUL_3820 | - | 1e-16 | 26.11% (203) | ring-hydroxylating dioxygenase, large terminal subunit |
| M. vanbaalenii PYR-1 | Mvan_4213 | - | 0.0 | 71.60% (419) | Rieske (2Fe-2S) domain-containing protein |
CLUSTAL 2.0.9 multiple sequence alignment
Mb3186c|M.bovis_AF2122/97 --MLSTDNRAELGDILTDIGDYLDDNPPALSLPP---AAYTS---SELWQ
Rv3161c|M.tuberculosis_H37Rv --MLSTDNRAELGDILTDIGDYLDDNPPALSLPP---AAYTS---SELWQ
MAV_0905|M.avium_104 -----------MPHFPKPAAGSWTENYPELGTGP---VDYTDSIDPAFFE
MMAR_4744|M.marinum_M -----------MPHFTKPAAGSWTQNYPELGTAP---VDYTDSIDPQYFA
MSMEG_4840|M.smegmatis_MC2_155 -----------MAHFPKPAVGSWTENWPELGTAP---VDYTDSIDPEQWK
Mvan_4213|M.vanbaalenii_PYR-1 -----------MAHFPKPAAGSWTENWPELGTAP---VDYTDSIDPEQWK
TH_3820|M.thermoresistible__bu -----------VAHFPKPAVGSWTENWPELGTAP---VDYTDSIDPEQWK
Mflv_2439|M.gilvum_PYR-GCK -----------MAFFKKPDVGSWTENWPELGTAP---VNYEDSIDPEQWK
MAB_4370c|M.abscessus_ATCC_199 MASLPRPVAVAQGALCRPQALVRDIDVSGLVDSHGGLLDRVIFTDGELYR
MUL_3820|M.ulcerans_Agy99 ------MDRDQLIDLTRRALKLARDKTTDLAPHQH-AVDAREYTSLDRHE
: . . *
Mb3186c|M.bovis_AF2122/97 LERERIFNRSWMLVAHVDQLAKTGDYVTVSV----AGEPVMVVRDVDGQL
Rv3161c|M.tuberculosis_H37Rv LERERIFNRSWMLVAHVDQVAKTGDYVTVSV----AGEPVMVVRDVDGQL
MAV_0905|M.avium_104 AEREAIFKKTWLNVGRVNRLPRTGSYFTRELPSAGKGTSVIIVKTKDGSV
MMAR_4744|M.marinum_M DEQRAIFRRTWLNVGRIERLPRTGSYFTKELPSTGAGMSVIVVKGKDGVI
MSMEG_4840|M.smegmatis_MC2_155 LEQQAIFRRTWLHVGRVERLPKTGSYFTREMPSVGKGTSIIVVKDKDGAV
Mvan_4213|M.vanbaalenii_PYR-1 LEQQAIFRKLWLHVGRVERLPKAGSYFTREMPSVGAGTSIIVNKDKDGQV
TH_3820|M.thermoresistible__bu LEQQAIFRRLWLHVGRVERLPKAGSYFTREMPSVGAGTSIIVNRDKDGTI
Mflv_2439|M.gilvum_PYR-GCK LEQQAIFRKTWLQMGRIELIPKKGSYITRELPSVGPGTSIVIVNDGE-QI
MAB_4370c|M.abscessus_ATCC_199 QELRRVFAPSWLFLAHADQFHRPGDFFTTYMG----EDPVIVSMDKNRRI
MUL_3820|M.ulcerans_Agy99 RDRAMVLD-SPQLVGYSSELPGPDTYRTKTIMG----RSILLTRAADRAV
: :: :. . . . : * : .::: : :
Mb3186c|M.bovis_AF2122/97 HALSPICRHR---LMLMVEPG---AGRIDTLTCQYHLWRYGLDGRLRGAP
Rv3161c|M.tuberculosis_H37Rv HALSPICRHR---LMLMVEPG---AGRIDTLTCQYHLWRYGLDGRLRGAP
MAV_0905|M.avium_104 KAYHNVCRHRGNKLVWNDFPNEETSGTCRQFTCKYHAWRYSLDGDLTFVQ
MMAR_4744|M.marinum_M RAFHNICRHRGNKLVWNDFPHEETAGTCRQFTCKYHAWRYGLDGELTFIQ
MSMEG_4840|M.smegmatis_MC2_155 RAFYNMCRHRGNKLVWNDYPGEEVSGTCRQFTCKYHAWRYALNGDLTFIQ
Mvan_4213|M.vanbaalenii_PYR-1 RAFYNLCRHRGNKLVWNDYPGEEVSGSCRQFTCKYHAWRYALDGELTFIQ
TH_3820|M.thermoresistible__bu RAFYNLCRHRGNKLVWNDYPGEEVSGTCRQFTCKYHGWRYALNGDLTFIQ
Mflv_2439|M.gilvum_PYR-GCK RAFYNLCRHRGNKLVWNDYPGEETSGTCRQFVCKYHAWRYDLKGDLKFVQ
MAB_4370c|M.abscessus_ATCC_199 RAFLNSCRHRGARVCRADY------GVTRGFTCTYHGWAYDLEGKLISVP
MUL_3820|M.ulcerans_Agy99 RAFDNVCLHRQSRVATGCG-------TALRFTCPYHGWTYDNGGRLVGLP
:* * ** : :.* ** * * * *
Mb3186c|M.bovis_AF2122/97 HMAANLD-FNRRECRLPQFA-VATWNGLVWINLDADA-EPIAAHLDLTDD
Rv3161c|M.tuberculosis_H37Rv HMAANLD-FNRRECRLPQFA-VATWNGLVWINLDADA-EPIAAHLDLTDD
MAV_0905|M.avium_104 QEEEFFD-LDKSNYGLAPVR-CEVWEGFIFINFDDNA-APLTDYLGPLAK
MMAR_4744|M.marinum_M QEDEFFD-VDKSQYGLKSVR-CEVWEGFIFINLDPAA-APLSEYLGSMAK
MSMEG_4840|M.smegmatis_MC2_155 QEDEFFD-VDKADYPLKPVR-CEIWEGFIFVNFDDDA-MPLEEYLGEFGQ
Mvan_4213|M.vanbaalenii_PYR-1 QEDEFFD-VDKADYPLKPVR-CEVWEGFIFVNFDDDA-EPLVDYLGDFAK
TH_3820|M.thermoresistible__bu QEDEFFD-VDKADYGLKPIR-CEVWEGFIFVNFDDDA-EPLEDYLGEFGQ
Mflv_2439|M.gilvum_PYR-GCK QEQEFFD-LDKQDYPLKPVR-CEVWEGFIFVNFDDDA-VSLEEYLGEFGQ
MAB_4370c|M.abscessus_ATCC_199 NEEGYPDSFSTDDWGLVEVPHVDSYHGLIFGTWNPAP-VGLRESLGDMAW
MUL_3820|M.ulcerans_Agy99 GREGFPG-IAVGSDGLTELP-ATEFAGFLWVSLHPAARLDVPAHLGPLAG
. . . * . : *::: . . . : *.
Mb3186c|M.bovis_AF2122/97 EFAGYRLGEMVQVESWS----HEWRANWKVAAENGHENYHVLGLHRQTLE
Rv3161c|M.tuberculosis_H37Rv EFAGYRLGEMVQVESWS----HEWRANWKVAAENGHENYHVLGLHRQTLE
MAV_0905|M.avium_104 SIEGYPFGEMTETYSYR----AEVGSNWKLFIDAFVEFYHAPILHQGQYT
MMAR_4744|M.marinum_M GLQGYPFHEMTEVYSYR----AEVGSNWKLFIDAFAEFYHAPVLHQGQYT
MSMEG_4840|M.smegmatis_MC2_155 GLKGYPFHEMTEVYSYR----AEINSNWKLFIDAFVEFYHAPILHMKQAT
Mvan_4213|M.vanbaalenii_PYR-1 GLEGYPFHEMTEVYSYR----AEINSNWKLFIDAFVEFYHAPILHMKQAT
TH_3820|M.thermoresistible__bu NLKGYPFHEMTEVYSYR----AEVNANWKLFIDAFTEFYHAPVLHNKQAV
Mflv_2439|M.gilvum_PYR-GCK GLKGYPFHEMTEHYSYR----AEIKSNWKLFIDAFVEFYHAPILHMKQAE
MAB_4370c|M.abscessus_ATCC_199 YMDAMLDHDSEGTVVVGGVHKWVLDGNWKLAAEQFATDWYHVNMSHASAL
MUL_3820|M.ulcerans_Agy99 ELQSWGIGRWSPLGEKV----LGSPIDWKLATDTFAENYHFATVHRRTFA
: . :**: : :: :
Mb3186c|M.bovis_AF2122/97 P---------------FVPGGGDLDVRQYSRWALRLRVPFTVPVEAKS--
Rv3161c|M.tuberculosis_H37Rv P---------------FVPGGGDLDVRQYSRWALRLRVPFTVPVEAKS--
MAV_0905|M.avium_104 KEEAAKIQKFGYEALHYELAGPHSLQSTWGGQAPPPDMSMVKPMDQVLRS
MMAR_4744|M.marinum_M KEEAAKIMKYGFEALHYELAGPHAMISVWGGQSPPADLKMVKPMDRVLRS
MSMEG_4840|M.smegmatis_MC2_155 PEEAAKLAKIGFEALHYDIKGDHSMISSWGGMSPPKDLNMVKPIERILHS
Mvan_4213|M.vanbaalenii_PYR-1 AEEAAKLAKVGFEALHYDIKDQHSMISSWGGMSPPKDLNMVKPIERILHS
TH_3820|M.thermoresistible__bu KEEAEKLAKYGYEALHYDIKGPHSMISSWGGMAPPKDPSMVKPIERILNS
Mflv_2439|M.gilvum_PYR-GCK KEEAEKLAKFGFEALHYDIKGDHSMISSWGGMSPPKDLNMVKPIERILHS
MAB_4370c|M.abscessus_ATCC_199 MVMAPQGKAPKDEIVHRTGRQYVDPNGHGAGFPVHPKNRFDARVVHQWTD
MUL_3820|M.ulcerans_Agy99 T----------------IARSNCTVFDSYG-----PHHRLIFPLNTIL--
. : :
Mb3186c|M.bovis_AF2122/97 ----------------LQLNEVQKSNLVV-LWT------FPNSALAIAG-
Rv3161c|M.tuberculosis_H37Rv ----------------LQLNEVQKSNLVV-LWT------FPNSALAIAG-
MAV_0905|M.avium_104 GLFGPWDKPE--IIEKLDLPPGVNVKRVP-QWGIDSWLFYPNFMLLIWEP
MMAR_4744|M.marinum_M GLFGPWDRPAP-ITDLEELPPGINPTKAK-QWGIDSFLFFPNFMLLIWEP
MSMEG_4840|M.smegmatis_MC2_155 GLFGPWDRPDIKGILPDELPPAVNPARQP-TWGQDSFEFFPNFTLLLWVP
Mvan_4213|M.vanbaalenii_PYR-1 GLFGPWDRPDIKGILPDELPPAVNPARQP-TWGQDSFEFFPNFTLLLWAP
TH_3820|M.thermoresistible__bu GLFGPWDRPDIKGIAPDELPPAINPSRQP-FWGQDSFEFFPNFTLLIWAP
Mflv_2439|M.gilvum_PYR-GCK GLFGPWDRPDIKGILPDELPPAINPGRHK-TWGQDSFEFFPNFTLLFWVP
MAB_4370c|M.abscessus_ATCC_199 YG---------------ALRERLGEARVQGPLTNGHATVFPNLSYLPVNG
MUL_3820|M.ulcerans_Agy99 -----------------ELENVPEGQWDPFHHMVVIYALFPNVVLSVTIA
* :**
Mb3186c|M.bovis_AF2122/97 ERVVWFGFIPQSIDRVQVLGGVLTTPELAADAAATAQTSQFVMAMINDED
Rv3161c|M.tuberculosis_H37Rv ERVVWFGFIPQSIDRVQVLGGVLTTPELAADAAATAQTSQFVMAMINDED
MAV_0905|M.avium_104 GWFLTYHYWPTAVDRHIFESTLYFVPPRNARERLAQELAAVTFKEYALQD
MMAR_4744|M.marinum_M GWYLTYHYWPTTVDKHIFETTLYFVPPRNARERLAQELAAVTFKEYALQD
MSMEG_4840|M.smegmatis_MC2_155 GWYLTYNYWPTDVDKHIFECNLYFVPPKNTRQRLAQELAAVTFKEYALQD
Mvan_4213|M.vanbaalenii_PYR-1 GWYLTYNYWPTAVDKHIFECNLYFVPPKNTRQRLSQELAAVTFKEYALQD
TH_3820|M.thermoresistible__bu GWYLTYNYWPTDVTKHIFEASLYFVPPKTTRQRLAQELAAVTFKEYALQD
Mflv_2439|M.gilvum_PYR-GCK GWYLTYNYWPTGVDTHIFEADLYFVPPKNLRERLSQELAAVTFKEYAFQD
MAB_4370c|M.abscessus_ATCC_199 SVRIWHPKGPDKMEVWAWTIVDRSMPEEVRDAQRLYNLRTFGPTGIFEQD
MUL_3820|M.ulcerans_Agy99 NGELFRIYPGDRPGSSITVHQNSTPLDLSDESVATGAQAVFDYAHATVRD
: . .*
Mb3186c|M.bovis_AF2122/97 RLGLEAVQVGAGSRFAERGHLSSKEWPGMLAFYRNLAMALVGDHP-----
Rv3161c|M.tuberculosis_H37Rv RLGLEAVQVGAGSRFAERGHLSSKEWPGMLAFYRNLAMALVGDHP-----
MAV_0905|M.avium_104 ANTLEATQTMIGTRAVKEFLLCDQEV--LIRHLHKTTGDYVKEYQ-----
MMAR_4744|M.marinum_M SNTLEATQTMIGTGVVKDFPLCDQEI--LLRHLHKMTGDYVKEYR-----
MSMEG_4840|M.smegmatis_MC2_155 ANTLEATQTQINTRAVTEFPLCDQEV--LLRHLHHTAHKYVDAYKAELAE
Mvan_4213|M.vanbaalenii_PYR-1 ANTLEATQTQIGTRAVTEFPLCDQEI--LLRHLHHTAHKYVDKYKLEQAA
TH_3820|M.thermoresistible__bu ANTLEATQTQIGTRAVTEFPLCDQEV--LLRHLHHTAHQYVDKYKEELAQ
Mflv_2439|M.gilvum_PYR-GCK ANTLEATQTQIGTRAVLDFPLCDQEV--LLRHLHHTAHKYVDRYKAELEA
MAB_4370c|M.abscessus_ATCC_199 DGENWSEVQSISRGFVTNSVALNYQMGLDSEREDGRHPGLTSELYSDAAG
MUL_3820|M.ulcerans_Agy99 EDYRLAETLQTNLESGARQRLVFGRNEPGLQHRHITWAQALETPG-----
: . .
Mb3186c|M.bovis_AF2122/97 -----------GAS----------
Rv3161c|M.tuberculosis_H37Rv -----------GAS----------
MAV_0905|M.avium_104 ---------NNGHVAAR-------
MMAR_4744|M.marinum_M ---------NNGHAK---------
MSMEG_4840|M.smegmatis_MC2_155 KTQ---KAESNGSAVTSEKDAAHV
Mvan_4213|M.vanbaalenii_PYR-1 K------AATNGSASKTEKEGANV
TH_3820|M.thermoresistible__bu K-----AQQTNGSKKEGARSTAHV
Mflv_2439|M.gilvum_PYR-GCK KSNGSSNGATTSSTTATQKDEVNA
MAB_4370c|M.abscessus_ATCC_199 RSFYARWRDLMNTKAWHEEHV---
MUL_3820|M.ulcerans_Agy99 ------------------------