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SISLLLEMATDAGPDRTAVVSDDTRLTTAELSALADGGAGVIAASGARHVAYVGTGGALLPLLLFASARA AVPVTPLNYRLSAEGLRALLDRLPDPLVVVDDEYRDAVGDSYRTMGSREFFEASRTAEPAAEFADPDSVG VVLFTSGTTSKPKAVELTHTNLTSYIMGTVEFASAEPDDAALICVPPYHIAGVSAALSNLYAGRKMVYLR HFDAEKWVRLVADEGVTSATVVPTMLDRIVTVLETTDTALPTLRTLAYGGSKVPLPLVRKALSLLPDVGF VNAYGLTETSSTIAVLTPDDHRAALAAADDAALRRLGSVGQPVPGIEVEVRAEDGTVLGPGETGELFVRG EQVSGKYTDIGSVLDENGWFPTKDVAYLDSEGYLFIGGRSDDTIIRGGENIAPAEIEDVLVEHPHVRDCA VVGADDPQWGQIIVAVVVPHQDTAPDPEDLRAHVRSQLRGSRTPDRVVFRDELPTNATGKVLRRELVSEL NQDRSPAQPAV*
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. gilvum PYR-GCK | Mflv_2385 | - | - | 100% (502) | AMP-dependent synthetase and ligase |
| M. gilvum PYR-GCK | Mflv_0027 | - | 1e-58 | 31.17% (494) | AMP-dependent synthetase and ligase |
| M. gilvum PYR-GCK | Mflv_5271 | - | 2e-58 | 33.18% (443) | AMP-dependent synthetase and ligase |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb3116 | fadD13 | 2e-53 | 29.59% (507) | chain-fatty-acid-CoA ligase |
| M. tuberculosis H37Rv | Rv3089 | fadD13 | 2e-53 | 29.59% (507) | chain-fatty-acid-CoA ligase |
| M. leprae Br4923 | MLBr_02546 | fadD2 | 1e-52 | 28.35% (508) | acyl-CoA synthetase |
| M. abscessus ATCC 19977 | MAB_2539c | - | 1e-52 | 31.65% (515) | fatty-acid-CoA ligase FadD |
| M. marinum M | MMAR_0466 | - | 0.0 | 73.19% (496) | long-chain-fatty-acid-CoA ligase |
| M. avium 104 | MAV_4950 | - | 0.0 | 74.02% (508) | AMP-dependent synthetase and ligase |
| M. smegmatis MC2 155 | MSMEG_4883 | - | 0.0 | 80.69% (492) | AMP-dependent synthetase and ligase |
| M. thermoresistible (build 8) | TH_2995 | - | 0.0 | 74.13% (491) | PUTATIVE AMP-dependent synthetase and ligase |
| M. ulcerans Agy99 | MUL_1115 | - | 0.0 | 72.58% (496) | long-chain-fatty-acid-CoA ligase |
| M. vanbaalenii PYR-1 | Mvan_4263 | - | 0.0 | 91.04% (502) | AMP-dependent synthetase and ligase |
CLUSTAL 2.0.9 multiple sequence alignment
Mflv_2385|M.gilvum_PYR-GCK -----------------------------------------------MSI
Mvan_4263|M.vanbaalenii_PYR-1 -----------------------------------------------MSI
MSMEG_4883|M.smegmatis_MC2_155 -----------------------------------------------MSI
MMAR_0466|M.marinum_M -----------------------------------------------MSV
MUL_1115|M.ulcerans_Agy99 -----------------------------------------------MSV
MAV_4950|M.avium_104 -----------------------------------------------MSI
TH_2995|M.thermoresistible__bu --------------------------------------------------
MLBr_02546|M.leprae_Br4923 -MTKLRHYLERGAAEVHYLRSIFESGALGFEPPLTYAAMAVAMRKWG-DL
MAB_2539c|M.abscessus_ATCC_199 MPNNLRESLRQWGAAIP---AAVTSGAFTPVSPRALASLVRSFWQFGPTP
Mb3116|M.bovis_AF2122/97 ----------------------------------------------MKNI
Rv3089|M.tuberculosis_H37Rv ----------------------------------------------MKNI
Mflv_2385|M.gilvum_PYR-GCK SLLLEMATDAGPDRTAVVS--DDTRLTTAELSALADGGAGVIAAS---GA
Mvan_4263|M.vanbaalenii_PYR-1 SLLLEMAQDAGPDRTAVVS--DDLRLTTAELSALADGGAGVIAAS---GA
MSMEG_4883|M.smegmatis_MC2_155 SLLLEMASSGDPDRTAVVS--DDTRLTAGELSTLADGAAGVIAGS---GA
MMAR_0466|M.marinum_M SLLLEMAASASPDRIAVVS--GDLRLTTQQLSDLADGGAGAIAAS---DA
MUL_1115|M.ulcerans_Agy99 SLLLEMAASASPDRIAVVS--GDLRLKTQQLSDLADGGAGAIAAS---DA
MAV_4950|M.avium_104 SLLLEMAASSNAERTAVVS--QDVRLTTQELSDLADGGAAVVAGS---NA
TH_2995|M.thermoresistible__bu -----MAVSGDPDRVAVID--RDVRLTTTELARLAGGGAQVIAGS---GV
MLBr_02546|M.leprae_Br4923 AMLPAMPARRSPHRAALID--EEGTLTYKELDRAAHALANGLIAKGVRGG
MAB_2539c|M.abscessus_ATCC_199 AMLLAASAIRFPDRAALID--DSGQLTYRDLQRRAEAVAAAVYARTPSAP
Mb3116|M.bovis_AF2122/97 GWMLRQRATVSPRLQAYVEPSTDVRMTYAQMNALANRCADVLTALGIAKG
Rv3089|M.tuberculosis_H37Rv GWMLRQRATVSPRLQAYVEPSTDVRMTYAQMNALANRCADVLTALGIAKG
. * :. . :. :: * * : .
Mflv_2385|M.gilvum_PYR-GCK RHVAYVGTGGALLPLLLFASARAAVPVTPLNYRLSAEGLRALLDRLPDPL
Mvan_4263|M.vanbaalenii_PYR-1 RHVAYVGAGGAMLPLLLFASARAAVPVTPLNYRLSADGLRALLDRLPDPL
MSMEG_4883|M.smegmatis_MC2_155 AHVAYVGTGGALLPLLLFASARAAIPFTPLNYRLSAEGLRELVDRLPTPL
MMAR_0466|M.marinum_M QHVVYVGTGGAMLPLLIFASARAGLPFTSINYRLSADGIAALIRRLPRPL
MUL_1115|M.ulcerans_Agy99 QHVVYVGTGGAMLPLLIFASARAGLPFTSINYRLSADGIAALIRRLPRPL
MAV_4950|M.avium_104 KHLVYVGTGGVLLPVLIFTAARAGVAFTPINYRLSAEGIRALIERLPEPL
TH_2995|M.thermoresistible__bu QHVAYVGLGGAMLPLLIFASTHATVPFTPLNYRLSAEGLHQLIERLPSPL
MLBr_02546|M.leprae_Br4923 DGIAILARNHRWFVIANYGCARVGARIILLNSEFSGPQIKEVSEREGAKV
MAB_2539c|M.abscessus_ATCC_199 RSVGIICRNHRGFAESMLAGAQLGAELVFINTELTPQQLGAILQRHEPDV
Mb3116|M.bovis_AF2122/97 DRVALLMPNSVEFCCLFYGAAKLGAVAVPINTRLAAPEVSFILSDSGSKV
Rv3089|M.tuberculosis_H37Rv DRVALLMPNSVEFCCLFYGAAKLGAVAVPINTRLAAPEVSFILSDSGSKV
: : . : :: :* .:: : : :
Mflv_2385|M.gilvum_PYR-GCK VVVDDEYRDAVGDSYR-TMGSREFFEASRTAEPAA------------EFA
Mvan_4263|M.vanbaalenii_PYR-1 VVVDDEYRDAVGDGYR-TIGSAEFLDAARTAEPAA------------EFA
MSMEG_4883|M.smegmatis_MC2_155 VIADGEYAGMLAGAGRPVLTSEEFLSQARTADPAA------------EFA
MMAR_0466|M.marinum_M VVVDERYRDTIAADQS--LSSQQFLELAGRAEPVA------------EFP
MUL_1115|M.ulcerans_Agy99 VVVDERYRETIAADQS--LSSQQFLELAGRAEPVA------------EFP
MAV_4950|M.avium_104 VVVDARYRDMVGEAPGGVMDSDDFLAAARAAEPAAD-----------AFA
TH_2995|M.thermoresistible__bu VIADDEYVDAVAGTGKQVMRSSEFVEAARAAEPLE------------GFA
MLBr_02546|M.leprae_Br4923 IIYDDEYTKVVSKAEPALGKLRALGVNPDEEEPPGSTDETLAELIARSSS
MAB_2539c|M.abscessus_ATCC_199 LVYDDEYSQAVEESQYQGLRVLAWRENPQENR------LTLDSLADQHHP
Mb3116|M.bovis_AF2122/97 VIYGAPSAPVIDAIRAQADPPGTVTDWIGADSLAERLRSAAADEPAVECG
Rv3089|M.tuberculosis_H37Rv VIYGAPSAPVIDAIRAQADPPGTVTDWIGADSLAERLRSAAADEPAVECG
:: . :
Mflv_2385|M.gilvum_PYR-GCK DPDS-----VGVVLFTSGTTSKPKAVELTH--TNLTSYIMGTVEFASAEP
Mvan_4263|M.vanbaalenii_PYR-1 DPDA-----VGVVLFTSGTTSKPKAVELSH--ANLTSYIMGTVEFASAEP
MSMEG_4883|M.smegmatis_MC2_155 DPDA-----VAVVLFTSGTTSRPKAVELTH--NNLTSYITGTVEFGSAAP
MMAR_0466|M.marinum_M DPDS-----VAIVLFTSGTTSQPKAVELTH--SNLTSYVTTTVEFGCAAP
MUL_1115|M.ulcerans_Agy99 DPDS-----VAIVLFTSGTTSQPKAVELTH--SNLTSYVTTTVEFGCAAP
MAV_4950|M.avium_104 DPDS-----VAIVLFTSGTTSQPKAVELSH--NNLTSYITGTVEFGAADE
TH_2995|M.thermoresistible__bu DPDS-----VGIVLFTSGTTSRPKAVELTH--NNLTSYVTGTVEFADADA
MLBr_02546|M.leprae_Br4923 APAPKVRKRASIIILTSGTTGTPKGANRNT-PASLAPLGG-ILSKVPFRA
MAB_2539c|M.abscessus_ATCC_199 KPPP-VRRPVKLTLLTSGTTGLAKGVPRAIKPRQLALMCVTAMATVRLRS
Mb3116|M.bovis_AF2122/97 GDDN------LFIMYTSGTTGHPKGVVHTH--ESVHSAASSWASTIDVRY
Rv3089|M.tuberculosis_H37Rv GDDN------LFIMYTSGTTGHPKGVVHTH--ESVHSAASSWASTIDVRY
. : *****. .*.. .:
Mflv_2385|M.gilvum_PYR-GCK DDAALICVPPYHIAGVSAALSNLYAGRKMVYLRHFDAEKWVRLVADEGVT
Mvan_4263|M.vanbaalenii_PYR-1 DDAALICVPPYHIAGVSAALSNLYAGRKMVYLTQFDAGRWVDLVTGEGVT
MSMEG_4883|M.smegmatis_MC2_155 EDAALICVPPYHIAGVSAAMSNLYAGRKMVYLRNFDAHRWVELVRTEGVT
MMAR_0466|M.marinum_M SDAALICVPPYHIAGVSAVLSNLYAGRKMVYLTNFDAHEWVRLTNAEQVT
MUL_1115|M.ulcerans_Agy99 SDAALICVPPYHIAGVSAVLSNLYAGRKMVYLNNFDAHEWVRLTNAEQVT
MAV_4950|M.avium_104 TDAALICVPPYHIAGVSAALSNLYAGRKMVYLPNFDAREWVRLINAENVT
TH_2995|M.thermoresistible__bu EDAALVCVPPYHIAGVGAALSNLYAGRKIVCLSRFDPAEWVRLVADEKVT
MLBr_02546|M.leprae_Br4923 HEVTLLPAPMFHALGYLHATLAMFLGSTLVLRRRFKPATVLEDIEKHQAT
MAB_2539c|M.abscessus_ATCC_199 RDRVLVAPPFFHGFGLAALLGPLALGGTVMCRRRFDAQQAMTDIRRQRVT
Mb3116|M.bovis_AF2122/97 RDRLLLPLPMFHVAALTTVIFSAMRGVTLISMPQFDATKVWSLIVEERVC
Rv3089|M.tuberculosis_H37Rv RDRLLLPLPMFHVAALTTVIFSAMRGVTLISMPQFDATKVWSLIVEERVC
: *: * :* . * .:: .*.. . .
Mflv_2385|M.gilvum_PYR-GCK SATVVPTMLDRIVTVLETTDT------ALPTLRTLAYGGSKVPLPLVRKA
Mvan_4263|M.vanbaalenii_PYR-1 SATVVPTMLDRIVSVLETRGT------TLPTLRTLAYGGSKVPLPLVRKA
MSMEG_4883|M.smegmatis_MC2_155 SATVVPTMLDRIVTALETTRA------ELPTLRNLAYGGSKVALPLVRKA
MMAR_0466|M.marinum_M TATVVPTMLDRIVAVLETGEH------KLPSLRNLAYGGSKVGLPLVRRA
MUL_1115|M.ulcerans_Agy99 TATVVPTMLDRIVAVLETGEH------KLPSLRNLAYGCSKVGLPLVRRA
MAV_4950|M.avium_104 TATVVPTMLDRIVTVLENGDPDTGAPIELPSLRNLAYGGSKVGLPLVRRA
TH_2995|M.thermoresistible__bu TATVVPTMLDRIVTELEARPR------ELPSLRNLAYGGSKVGLPLVRKA
MLBr_02546|M.leprae_Br4923 AMVVVPVMLSRILDTLENLETK----SDLSSLRVVFVSGSQLGAELATRA
MAB_2539c|M.abscessus_ATCC_199 VLMAVPVMLQRILALADLDRRD----ARQLSLRMIVTGAAPISSSTVSGV
Mb3116|M.bovis_AF2122/97 IGGAVPAILNFMRQVPEFAELD------APDFRYFITGGAPMPEALIK--
Rv3089|M.tuberculosis_H37Rv IGGAVPAILNFMRQVPEFAELD------APDFRYFITGGAPMPEALIK--
.**.:*. : : :* . . : :
Mflv_2385|M.gilvum_PYR-GCK LSLLPDVGFVNAYGLTETSSTIAVLTPDDHRAALAAADDAALRRLGSVGQ
Mvan_4263|M.vanbaalenii_PYR-1 LSLLPAVGFVNAYGLTETSSTIAVLTPDDHRAALAASDEAAIRRLGSVGQ
MSMEG_4883|M.smegmatis_MC2_155 LELMPNVGFVNAYGLTETSSTIAVLGPDDHRAALASDDPGVTRRLGSVGQ
MMAR_0466|M.marinum_M LELLPDVGFVNAYGLTETSSTIAVLTPDDHRAAQSAAAAGLARRLGSVGR
MUL_1115|M.ulcerans_Agy99 LELLPDVGFVNAYRLTETSSTIAVLTPDDHRAAQSAAAAGLARRLGSVGR
MAV_4950|M.avium_104 LELLPHVGFVNAYGLTETSSTIAVLTPDDHRAALAATTPEAARRLGSVGQ
TH_2995|M.thermoresistible__bu LELLPDVGFVNAYGLTETSSTIAVLGPEDHRIALASDDEKVVRRLGSVGQ
MLBr_02546|M.leprae_Br4923 LEELGPV-IYNMYGSTEIA------------FATIAGPKDLQINSATVGP
MAB_2539c|M.abscessus_ATCC_199 LEAFGPI-LVNGYGSTEAG------------VVAIASPKDLVASPNTIGR
Mb3116|M.bovis_AF2122/97 IYAAKNIEVVQGYALTESCG-----------GGTLLLSEDALRKAGSAGR
Rv3089|M.tuberculosis_H37Rv IYAAKNIEVVQGYALTESCG-----------GGTLLLSEDALRKAGSAGR
: : . : * ** : *
Mflv_2385|M.gilvum_PYR-GCK PVPGIEVEVRAEDGTVLGPGETGELFVRGEQVSGKYTDIGS-----VLDE
Mvan_4263|M.vanbaalenii_PYR-1 PVPGIEVEIRADDGTVLGAGETGELFVRGEQVSGKYTDIGS-----VLDE
MSMEG_4883|M.smegmatis_MC2_155 VVPGIEVQIRGEDGTVLGPGETGELFVRGEQVSGRYTEIGS-----VLDE
MMAR_0466|M.marinum_M PVPGVELQIRGQDGRVLGPGETGELFVRGGQVSGRYTGIGS-----VLDG
MUL_1115|M.ulcerans_Agy99 PVPGVELQIRGQDGRVLGPGETGELFVRGEQVSGRYTGIGS-----VLDG
MAV_4950|M.avium_104 AVAGIELQIRDEAGNVLPPGETGELFVRGEQVSGRYTGIGS-----VLDE
TH_2995|M.thermoresistible__bu PVPGIEIQIRSKNGEVLGPGETGELFVRGPQVSGRYTGIGS-----VLDD
MLBr_02546|M.leprae_Br4923 VVKGVKVKILDDSGKEVPRGQVGRIFVGNAFPFEGYTGGGG-----KQII
MAB_2539c|M.abscessus_ATCC_199 TALGVSVRILREDRRPAAPGETGMIFVRGGLEYEGYTPDKATRPTAKEIV
Mb3116|M.bovis_AF2122/97 ATMFTDVAVRGDDGVIREHGEGEVVIKSDILLKEYWNRPEATR---DAFD
Rv3089|M.tuberculosis_H37Rv ATMFTDVAVRGDDGVIREHGEGEVVIKSDILLKEYWNRPEATR---DAFD
. .: : . *: :: . :. .
Mflv_2385|M.gilvum_PYR-GCK NGWFPTKDVAYLDSEGYLFIGGRSDDTIIRGGENIAPAEIEDVLVEHPHV
Mvan_4263|M.vanbaalenii_PYR-1 QGWFPTKDVAFLDEEGYLFIGGRSDDTIIRGGENIAPAEIEDVLVEHPHV
MSMEG_4883|M.smegmatis_MC2_155 DGWFPTKDVAMLDQDGYLFIGGRSDDTIIRGGENIAPAEIEDVLVEHPDV
MMAR_0466|M.marinum_M HGWFGTKDIAMLDEDGYLFIGGRSDDTIIRGGENIAPAELEEVLIEHPQV
MUL_1115|M.ulcerans_Agy99 HGWFGTKDIAMLDEDGYLFIGGRSDDTIIRGGENIAPAELEEVLIEHPQV
MAV_4950|M.avium_104 DGWFPTRDIAMLDDEGYLFIGGRSDDTIIRGGENIAPAELEEVLVEHPHV
TH_2995|M.thermoresistible__bu DGWFPTKDVAMVDEDGYLFIGGRSDDTIIRGGENIAPAEIENVLVEHPQV
MLBr_02546|M.leprae_Br4923 DGLLSSGDVGYFDEHDLLYISGRDDEMIVSGGENVFPAEVEDLISGHPEV
MAB_2539c|M.abscessus_ATCC_199 DGHVNTGDMGHFDADGRLYIDGRSDDMIVSGGENVFPGEVEDRLTTYPGI
Mb3116|M.bovis_AF2122/97 NGWFRTGDIGEIDDEGYLYIKDRLKDMIISGGENVYPAEIESVIIGVPGV
Rv3089|M.tuberculosis_H37Rv NGWFRTGDIGEIDDEGYLYIKDRLKDMIISGGENVYPAEIESVIIGVPGV
.* . : *:. .* .. *:* .* .: *: ****: *.*:*. : * :
Mflv_2385|M.gilvum_PYR-GCK RDCAVVGADDPQWGQIIVAVVVPHQDTAPD-PEDLRAHVRSQLRGSRTPD
Mvan_4263|M.vanbaalenii_PYR-1 RDCAVVGADDPEWGQIIVAVVVAQPGTEPD-VEDLRSHVRSQLRGSRTPD
MSMEG_4883|M.smegmatis_MC2_155 RDVAVVGPEDPQWGQIIVAVVVPADGAEPD-ADVLREHVRKHLRGSRTPD
MMAR_0466|M.marinum_M RDVAVVGVEDAQWGQEIVAVVVPVAGVDPD-PEELREFVRRSLRGSRTPD
MUL_1115|M.ulcerans_Agy99 RDVAVVGVEDAQWGQEIVAVVVPVAGVDPD-PEELREFVRRSLRGSRTPD
MAV_4950|M.avium_104 RDVAVVGVEDPQWGQAIVAVVVPAAGVEPD-PEELREFVRKSLRGSRTPD
TH_2995|M.thermoresistible__bu REAVVVGPEDPQWGQILVAVVVPAEGETPD-PEELRAHVRKHLRGSRTPD
MLBr_02546|M.leprae_Br4923 VEATAIGVDDKEWGARLRAFVVKKQDATID-EDAIKAYVRDHLARYKVPR
MAB_2539c|M.abscessus_ATCC_199 TDAVVIGVPDDDFGQVLHAFVVAADGAPAPSEEVLKTHVRQGLERYKVPK
Mb3116|M.bovis_AF2122/97 SEVAVIGLPDEKWGEIAAAIVVADQNEVSE--QQIVEYCGTRLARYKLPK
Rv3089|M.tuberculosis_H37Rv SEVAVIGLPDEKWGEIAAAIVVADQNEVSE--QQIVEYCGTRLARYKLPK
: ..:* * .:* *.** . : : . * : *
Mflv_2385|M.gilvum_PYR-GCK RVVFRDELPTNATGKVLRRELVSELNQDRSPAQPAV
Mvan_4263|M.vanbaalenii_PYR-1 RVVFRDELPTNATGKVLRRELVDELNQDPSPAQPAV
MSMEG_4883|M.smegmatis_MC2_155 RVVFRAELPTNATGKVLRRQLVDEL-------QPIS
MMAR_0466|M.marinum_M RVVFRHELPTTATGKILRREIIEELAGTKS------
MUL_1115|M.ulcerans_Agy99 RVVFRRELPTTATGKILRREIIEELAGTKS------
MAV_4950|M.avium_104 EVVFRDELPTTATGKVLRREIIATLAGLRAAPAQQ-
TH_2995|M.thermoresistible__bu KVVFRTELPVNALGKVLRRQLVDELQTKEPA-----
MLBr_02546|M.leprae_Br4923 EVIFLEELPRNPTGKVLKRELRDFEVH---------
MAB_2539c|M.abscessus_ATCC_199 RFIVLDEIPRNASGKVLRAKLNSPGAC---------
Mb3116|M.bovis_AF2122/97 KVIFAEAIPRNPTGKILKTVLREQYSATVPK-----
Rv3089|M.tuberculosis_H37Rv KVIFAEAIPRNPTGKILKTVLREQYSATVPK-----
..:. :* .. **:*: :