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MSISLLLEMASSGDPDRTAVVSDDTRLTAGELSTLADGAAGVIAGSGAAHVAYVGTGGALLPLLLFASAR AAIPFTPLNYRLSAEGLRELVDRLPTPLVIADGEYAGMLAGAGRPVLTSEEFLSQARTADPAAEFADPDA VAVVLFTSGTTSRPKAVELTHNNLTSYITGTVEFGSAAPEDAALICVPPYHIAGVSAAMSNLYAGRKMVY LRNFDAHRWVELVRTEGVTSATVVPTMLDRIVTALETTRAELPTLRNLAYGGSKVALPLVRKALELMPNV GFVNAYGLTETSSTIAVLGPDDHRAALASDDPGVTRRLGSVGQVVPGIEVQIRGEDGTVLGPGETGELFV RGEQVSGRYTEIGSVLDEDGWFPTKDVAMLDQDGYLFIGGRSDDTIIRGGENIAPAEIEDVLVEHPDVRD VAVVGPEDPQWGQIIVAVVVPADGAEPDADVLREHVRKHLRGSRTPDRVVFRAELPTNATGKVLRRQLVD ELQPIS
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. smegmatis MC2 155 | MSMEG_4883 | - | - | 100% (496) | AMP-dependent synthetase and ligase |
| M. smegmatis MC2 155 | MSMEG_3694 | - | 9e-68 | 35.43% (494) | feruloyl-CoA synthetase |
| M. smegmatis MC2 155 | MSMEG_4065 | - | 5e-58 | 30.91% (508) | feruloyl-CoA synthetase |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb0276 | fadD2 | 4e-54 | 31.87% (502) | acyl-CoA synthetase |
| M. gilvum PYR-GCK | Mflv_2385 | - | 0.0 | 80.69% (492) | AMP-dependent synthetase and ligase |
| M. tuberculosis H37Rv | Rv0270 | fadD2 | 4e-54 | 31.87% (502) | acyl-CoA synthetase |
| M. leprae Br4923 | MLBr_02546 | fadD2 | 5e-53 | 30.49% (515) | acyl-CoA synthetase |
| M. abscessus ATCC 19977 | MAB_4559 | - | 2e-54 | 32.89% (377) | putative long-chain-fatty-acid CoA ligase |
| M. marinum M | MMAR_0466 | - | 0.0 | 73.78% (492) | long-chain-fatty-acid-CoA ligase |
| M. avium 104 | MAV_2574 | - | 0.0 | 75.10% (490) | feruloyl-CoA synthetase |
| M. thermoresistible (build 8) | TH_2995 | - | 0.0 | 77.11% (485) | PUTATIVE AMP-dependent synthetase and ligase |
| M. ulcerans Agy99 | MUL_1115 | - | 0.0 | 73.37% (492) | long-chain-fatty-acid-CoA ligase |
| M. vanbaalenii PYR-1 | Mvan_4263 | - | 0.0 | 80.08% (492) | AMP-dependent synthetase and ligase |
CLUSTAL 2.0.9 multiple sequence alignment
Mflv_2385|M.gilvum_PYR-GCK --------------------------------------------------
Mvan_4263|M.vanbaalenii_PYR-1 --------------------------------------------------
MSMEG_4883|M.smegmatis_MC2_155 --------------------------------------------------
TH_2995|M.thermoresistible__bu --------------------------------------------------
MAV_2574|M.avium_104 --------------------------------------------------
MMAR_0466|M.marinum_M --------------------------------------------------
MUL_1115|M.ulcerans_Agy99 --------------------------------------------------
Mb0276|M.bovis_AF2122/97 MPNLTDLPGQAVSKLQKSIGQYVARGTAELHYLRKIIESGAIGLEPPLNY
Rv0270|M.tuberculosis_H37Rv MPNLTDLPGQAVSKLQKSIGQYVARGTAELHYLRKIIESGAIGLEPPLNY
MLBr_02546|M.leprae_Br4923 -----------MTKLR----HYLERGAAEVHYLRSIFESGALGFEPPLTY
MAB_4559|M.abscessus_ATCC_1997 --------------------------------------------------
Mflv_2385|M.gilvum_PYR-GCK ----------MSISLLLEMATDAGPDRTAVVSDDTRLTTAELSALADGGA
Mvan_4263|M.vanbaalenii_PYR-1 ----------MSISLLLEMAQDAGPDRTAVVSDDLRLTTAELSALADGGA
MSMEG_4883|M.smegmatis_MC2_155 ----------MSISLLLEMASSGDPDRTAVVSDDTRLTAGELSTLADGAA
TH_2995|M.thermoresistible__bu ------------------MAVSGDPDRVAVIDRDVRLTTTELARLAGGGA
MAV_2574|M.avium_104 --------------MLLEMAASGDPDRVAVVDGDIRLTTVDLSELADGGA
MMAR_0466|M.marinum_M ----------MSVSLLLEMAASASPDRIAVVSGDLRLTTQQLSDLADGGA
MUL_1115|M.ulcerans_Agy99 ----------MSVSLLLEMAASASPDRIAVVSGDLRLKTQQLSDLADGGA
Mb0276|M.bovis_AF2122/97 AALAADIRKWGEVGMLPSHNARRAPNRAAVIDEEGTLTFSELDEAAHAVA
Rv0270|M.tuberculosis_H37Rv AALAADIRKWGEVGMLPSHNARRAPNRAAVIDEEGTLTFSELDEAAHAVA
MLBr_02546|M.leprae_Br4923 AAMAVAMRKWGDLAMLPAMPARRSPHRAALIDEEGTLTYKELDRAAHALA
MAB_4559|M.abscessus_ATCC_1997 ----------MQFTQWLHRALQQYPDRLITIDQGREHTVEQFADRVARLS
*.* :. . :: . :
Mflv_2385|M.gilvum_PYR-GCK GVIAASGAR---HVAYVGTGGALLPLLLFASARAAVPVTPLNYRLSAEGL
Mvan_4263|M.vanbaalenii_PYR-1 GVIAASGAR---HVAYVGAGGAMLPLLLFASARAAVPVTPLNYRLSADGL
MSMEG_4883|M.smegmatis_MC2_155 GVIAGSGAA---HVAYVGTGGALLPLLLFASARAAIPFTPLNYRLSAEGL
TH_2995|M.thermoresistible__bu QVIAGSGVQ---HVAYVGLGGAMLPLLIFASTHATVPFTPLNYRLSAEGL
MAV_2574|M.avium_104 GVIAASGAS---HVAYVGTGGVLLPLLLFSSARANTAFTPLNYRLSPEGL
MMAR_0466|M.marinum_M GAIAASDAQ---HVVYVGTGGAMLPLLIFASARAGLPFTSINYRLSADGI
MUL_1115|M.ulcerans_Agy99 GAIAASDAQ---HVVYVGTGGAMLPLLIFASARAGLPFTSINYRLSADGI
Mb0276|M.bovis_AF2122/97 NGLLAKGVRAGDGVAILARNHRWFVIANYGAARVGARIILLNSEFSGPQI
Rv0270|M.tuberculosis_H37Rv NGLLAKGVRAGDGVAILARNHRWFVIANYGAARVGARIILLNSEFSGPQI
MLBr_02546|M.leprae_Br4923 NGLIAKGVRGGDGIAILARNHRWFVIANYGCARVGARIILLNSEFSGPQI
MAB_4559|M.abscessus_ATCC_1997 AGLRANGVRPGDRVGMLSLNSDRYIEYLTAVPWLGAALNAVNIRWSLAEI
: .... : :. . . . . :* . * :
Mflv_2385|M.gilvum_PYR-GCK RALLDRLPDPLVVVDDEYRDAVGDSYR-T-----------------MGSR
Mvan_4263|M.vanbaalenii_PYR-1 RALLDRLPDPLVVVDDEYRDAVGDGYR-T-----------------IGSA
MSMEG_4883|M.smegmatis_MC2_155 RELVDRLPTPLVIADGEYAGMLAGAGRPV-----------------LTSE
TH_2995|M.thermoresistible__bu HQLIERLPSPLVIADDEYVDAVAGTGKQV-----------------MRSS
MAV_2574|M.avium_104 RELIDRLPSPLVVVDAEYRDIGAGFGQRV-----------------MTSD
MMAR_0466|M.marinum_M AALIRRLPRPLVVVDERYRDTIAADQS-------------------LSSQ
MUL_1115|M.ulcerans_Agy99 AALIRRLPRPLVVVDERYRETIAADQS-------------------LSSQ
Mb0276|M.bovis_AF2122/97 KEVSDREGAKVIIYDDEYTKAVSLAQPPLGKLRALGVNPDDDKPSGSSDE
Rv0270|M.tuberculosis_H37Rv KEVSDREGAKVIIYDDEYTKAVSLAQPPLGKLRALGVNPDDDKPSGSSDE
MLBr_02546|M.leprae_Br4923 KEVSEREGAKVIIYDDEYTKVVSKAEPALGKLRALGVNPDEEEPPGSTDE
MAB_4559|M.abscessus_ATCC_1997 GYSLRESGTRVLLVDDTFKAAAAPLRAACACLETVIYCGAG--PAPQDMI
. ::: * : .
Mflv_2385|M.gilvum_PYR-GCK EFFEASRTAEPAAEFADP-DSVGVVLFTSGTTSKPKAVELTHTNLTSYIM
Mvan_4263|M.vanbaalenii_PYR-1 EFLDAARTAEPAAEFADP-DAVGVVLFTSGTTSKPKAVELSHANLTSYIM
MSMEG_4883|M.smegmatis_MC2_155 EFLSQARTADPAAEFADP-DAVAVVLFTSGTTSRPKAVELTHNNLTSYIT
TH_2995|M.thermoresistible__bu EFVEAARAAEPLEGFADP-DSVGIVLFTSGTTSRPKAVELTHNNLTSYVT
MAV_2574|M.avium_104 EFIEAARSTEPVSVFADP-DDVAVVLFTSGTTSRPKAVELTHNNLTSYVT
MMAR_0466|M.marinum_M QFLELAGRAEPVAEFPDP-DSVAIVLFTSGTTSQPKAVELTHSNLTSYVT
MUL_1115|M.ulcerans_Agy99 QFLELAGRAEPVAEFPDP-DSVAIVLFTSGTTSQPKAVELTHSNLTSYVT
Mb0276|M.bovis_AF2122/97 TLAELIAHSSTAPAPKAS-RRASIIILTSGTTGTPKGANRNTPPTLAPIG
Rv0270|M.tuberculosis_H37Rv TLAELIAHSSTAPAPKAS-RRASIIILTSGTTGTPKGANRNTPPTLAPIG
MLBr_02546|M.leprae_Br4923 TLAELIARSSSAPAPKVR-KRASIIILTSGTTGTPKGANRNTPASLAPLG
MAB_4559|M.abscessus_ATCC_1997 GYEDLLASHEPIDDTRTGGDSLLGVFYTGGTTGNPKGVMLSHNNVLASAM
. .. :: *.***. **.. . :
Mflv_2385|M.gilvum_PYR-GCK GTVEFASAEP-DDAALICVPPYHIAGVSAALSNLYAGRKMVYLRHFDAEK
Mvan_4263|M.vanbaalenii_PYR-1 GTVEFASAEP-DDAALICVPPYHIAGVSAALSNLYAGRKMVYLTQFDAGR
MSMEG_4883|M.smegmatis_MC2_155 GTVEFGSAAP-EDAALICVPPYHIAGVSAAMSNLYAGRKMVYLRNFDAHR
TH_2995|M.thermoresistible__bu GTVEFADADA-EDAALVCVPPYHIAGVGAALSNLYAGRKIVCLSRFDPAE
MAV_2574|M.avium_104 GTVEFGSADP-ADAALVCVPPYHIAGVGAALSNLYAGRAIVYLRRFDPHE
MMAR_0466|M.marinum_M TTVEFGCAAP-SDAALICVPPYHIAGVSAVLSNLYAGRKMVYLTNFDAHE
MUL_1115|M.ulcerans_Agy99 TTVEFGCAAP-SDAALICVPPYHIAGVSAVLSNLYAGRKMVYLNNFDAHE
Mb0276|M.bovis_AF2122/97 GILSHVPFKA-GEVTLLPSPMFHALGYMHAALAMFLGSTLVLRRRFKPAL
Rv0270|M.tuberculosis_H37Rv GILSHVPFKA-GEVTLLPSPMFHALGYMHAALAMFLGSTLVLRRRFKPAL
MLBr_02546|M.leprae_Br4923 GILSKVPFRA-HEVTLLPAPMFHALGYLHATLAMFLGSTLVLRRRFKPAT
MAB_4559|M.abscessus_ATCC_1997 GSLSTGNFLTRGGRLLHSAPMFHMADFSAVLAGNLSGSTHVIVPSFSPQG
:. . * * :* . . * * *..
Mflv_2385|M.gilvum_PYR-GCK WVRLVADEGVTSATVVPTMLDRIVTVLETTDTA--LPTLRTLAYGGSKVP
Mvan_4263|M.vanbaalenii_PYR-1 WVDLVTGEGVTSATVVPTMLDRIVSVLETRGTT--LPTLRTLAYGGSKVP
MSMEG_4883|M.smegmatis_MC2_155 WVELVRTEGVTSATVVPTMLDRIVTALETTRAE--LPTLRNLAYGGSKVA
TH_2995|M.thermoresistible__bu WVRLVADEKVTTATVVPTMLDRIVTELEARPRE--LPSLRNLAYGGSKVG
MAV_2574|M.avium_104 WVRLVRDERVTTATVVPTMLSRIVAVLEEQSAE--LPTLRNFAYGGSKVA
MMAR_0466|M.marinum_M WVRLTNAEQVTTATVVPTMLDRIVAVLETGEHK--LPSLRNLAYGGSKVG
MUL_1115|M.ulcerans_Agy99 WVRLTNAEQVTTATVVPTMLDRIVAVLETGEHK--LPSLRNLAYGCSKVG
Mb0276|M.bovis_AF2122/97 VLEDIEKHKATSMVVVPVMLSRILDQLEKTEPKPDLSSLKIVFVSGSQLG
Rv0270|M.tuberculosis_H37Rv VLEDIEKHKATSMVVVPVMLSRILDQLEKTEPKPDLSSLKIVFVSGSQLG
MLBr_02546|M.leprae_Br4923 VLEDIEKHQATAMVVVPVMLSRILDTLENLETKSDLSSLRVVFVSGSQLG
MAB_4559|M.abscessus_ATCC_1997 VLDAIVAHDVQDMLLVPTMIQMLVDHPGAAQLD--LSGIRSITYGASVIS
: . . :**.*:. :: *. :: . . * :
Mflv_2385|M.gilvum_PYR-GCK LPLVRKALSLLPDVGFVNAYGLTETSSTIAVLTPDDHRAALAAADDAALR
Mvan_4263|M.vanbaalenii_PYR-1 LPLVRKALSLLPAVGFVNAYGLTETSSTIAVLTPDDHRAALAASDEAAIR
MSMEG_4883|M.smegmatis_MC2_155 LPLVRKALELMPNVGFVNAYGLTETSSTIAVLGPDDHRAALASDDPGVTR
TH_2995|M.thermoresistible__bu LPLVRKALELLPDVGFVNAYGLTETSSTIAVLGPEDHRIALASDDEKVVR
MAV_2574|M.avium_104 RPLVRRALDLLPQVGFVNAYGLTETSSTIAVLTPEDHRLAHEARDDAVAR
MMAR_0466|M.marinum_M LPLVRRALELLPDVGFVNAYGLTETSSTIAVLTPDDHRAAQSAAAAGLAR
MUL_1115|M.ulcerans_Agy99 LPLVRRALELLPDVGFVNAYRLTETSSTIAVLTPDDHRAAQSAAAAGLAR
Mb0276|M.bovis_AF2122/97 AELATRALGDLGPVIYN-------------MYGSTEVAFATIAGPKDLQF
Rv0270|M.tuberculosis_H37Rv AELATRALGDLGPVIYN-------------MYGSTEVAFATIAGPKDLQF
MLBr_02546|M.leprae_Br4923 AELATRALEELGPVIYN-------------MYGSTEIAFATIAGPKDLQI
MAB_4559|M.abscessus_ATCC_1997 EAVLQRATRVFPNARFTQAYG---------MTEVSPVATLLLPDDHDDPA
: :* : . : : .
Mflv_2385|M.gilvum_PYR-GCK RLGSVGQPVPGIEVEVRAEDGTVLGPGETGELFVRGEQVSGKYTDI---G
Mvan_4263|M.vanbaalenii_PYR-1 RLGSVGQPVPGIEVEIRADDGTVLGAGETGELFVRGEQVSGKYTDI---G
MSMEG_4883|M.smegmatis_MC2_155 RLGSVGQVVPGIEVQIRGEDGTVLGPGETGELFVRGEQVSGRYTEI---G
TH_2995|M.thermoresistible__bu RLGSVGQPVPGIEIQIRSKNGEVLGPGETGELFVRGPQVSGRYTGI---G
MAV_2574|M.avium_104 RLTSVGRPVPGIEVQIRAEDGTVLGPGETGELFVRGPQVSGRYTEI---G
MMAR_0466|M.marinum_M RLGSVGRPVPGVELQIRGQDGRVLGPGETGELFVRGGQVSGRYTGI---G
MUL_1115|M.ulcerans_Agy99 RLGSVGRPVPGVELQIRGQDGRVLGPGETGELFVRGEQVSGRYTGI---G
Mb0276|M.bovis_AF2122/97 NPSTVGPVVKGVTVKILDENGNEVPQGAVGRIFVGNAFPFEGYTGG---G
Rv0270|M.tuberculosis_H37Rv NPSTVGPVVKGVTVKILDENGNEVPQGAVGRIFVGNAFPFEGYTGG---G
MLBr_02546|M.leprae_Br4923 NSATVGPVVKGVKVKILDDSGKEVPRGQVGRIFVGNAFPFEGYTGG---G
MAB_4559|M.abscessus_ATCC_1997 LLRSAGRAAPHCEVRIVDPDDNEVPRGEIGEVIVKGDNVMLGYWELPEES
:.* . :.: .. : * *.::* . * .
Mflv_2385|M.gilvum_PYR-GCK SVLDENGWFPTKDVAYLDSEGYLFIGGRSDDTIIRGGENIAPAEIEDVLV
Mvan_4263|M.vanbaalenii_PYR-1 SVLDEQGWFPTKDVAFLDEEGYLFIGGRSDDTIIRGGENIAPAEIEDVLV
MSMEG_4883|M.smegmatis_MC2_155 SVLDEDGWFPTKDVAMLDQDGYLFIGGRSDDTIIRGGENIAPAEIEDVLV
TH_2995|M.thermoresistible__bu SVLDDDGWFPTKDVAMVDEDGYLFIGGRSDDTIIRGGENIAPAEIENVLV
MAV_2574|M.avium_104 SVLDDHGWFATRDVAMLDEDGYLFIGGRSDDTIIRGGENIAPSEVEDVLI
MMAR_0466|M.marinum_M SVLDGHGWFGTKDIAMLDEDGYLFIGGRSDDTIIRGGENIAPAELEEVLI
MUL_1115|M.ulcerans_Agy99 SVLDGHGWFGTKDIAMLDEDGYLFIGGRSDDTIIRGGENIAPAELEEVLI
Mb0276|M.bovis_AF2122/97 GKQIIDGLLSSGDVGYFDERGLLYVSGRDDEMIVSGGENVFPAEVEDLIS
Rv0270|M.tuberculosis_H37Rv GKQIIDGLLSSGDVGYFDERGLLYVSGRDDEMIVSGGENVFPAEVEDLIS
MLBr_02546|M.leprae_Br4923 GKQIIDGLLSSGDVGYFDEHDLLYISGRDDEMIVSGGENVFPAEVEDLIS
MAB_4559|M.abscessus_ATCC_1997 AAAIRDGWMHTGDAGRMDDRGYVYIVDRIKDMVVTGGENVYSAEVENALA
. .* : : * . .*. . ::: .* .: :: ****: .:*:*: :
Mflv_2385|M.gilvum_PYR-GCK EHPHVRDCAVVGADDPQWGQIIVAVVVPHQDTAPDPEDLRAHVRSQLRGS
Mvan_4263|M.vanbaalenii_PYR-1 EHPHVRDCAVVGADDPEWGQIIVAVVVAQPGTEPDVEDLRSHVRSQLRGS
MSMEG_4883|M.smegmatis_MC2_155 EHPDVRDVAVVGPEDPQWGQIIVAVVVPADGAEPDADVLREHVRKHLRGS
TH_2995|M.thermoresistible__bu EHPQVREAVVVGPEDPQWGQILVAVVVPAEGETPDPEELRAHVRKHLRGS
MAV_2574|M.avium_104 EHPDVREVVVVGLDDAEWGQAIVAVIVPESGTAPAPERLREFARSKLRGS
MMAR_0466|M.marinum_M EHPQVRDVAVVGVEDAQWGQEIVAVVVPVAGVDPDPEELREFVRRSLRGS
MUL_1115|M.ulcerans_Agy99 EHPQVRDVAVVGVEDAQWGQEIVAVVVPVAGVDPDPEELREFVRRSLRGS
Mb0276|M.bovis_AF2122/97 GHPDVVEAAAIGVDDKEFGARLRAFVVKKPGADLDEDTIKQYVRDHLARY
Rv0270|M.tuberculosis_H37Rv GHPDVVEAAAIGVDDKEFGARLRAFVVKKPGADLDEDTIKQYVRDHLARY
MLBr_02546|M.leprae_Br4923 GHPEVVEATAIGVDDKEWGARLRAFVVKKQDATIDEDAIKAYVRDHLARY
MAB_4559|M.abscessus_ATCC_1997 KHPAVAACAVIGIPDEQWGERVHAVVVKQAQSECCDGDLQEHCREHIANY
** * ..:* * ::* : *.:* :: . * :
Mflv_2385|M.gilvum_PYR-GCK RTPDRVVFRDELPTNATGKVLRRELVSELNQDRSPAQPAV
Mvan_4263|M.vanbaalenii_PYR-1 RTPDRVVFRDELPTNATGKVLRRELVDELNQDPSPAQPAV
MSMEG_4883|M.smegmatis_MC2_155 RTPDRVVFRAELPTNATGKVLRRQLVDEL-------QPIS
TH_2995|M.thermoresistible__bu RTPDKVVFRTELPVNALGKVLRRQLVDELQT----KEPA-
MAV_2574|M.avium_104 RTPDRIVFRDELPTTPTGKVLRREILEDLKP----AAAEH
MMAR_0466|M.marinum_M RTPDRVVFRHELPTTATGKILRREIIEELAG----TKS--
MUL_1115|M.ulcerans_Agy99 RTPDRVVFRRELPTTATGKILRREIIEELAG----TKS--
Mb0276|M.bovis_AF2122/97 KVPREVIFLDELPRNPTGKVLKRELRKL------------
Rv0270|M.tuberculosis_H37Rv KVPREVIFLDELPRNPTGKVLKRELRKL------------
MLBr_02546|M.leprae_Br4923 KVPREVIFLEELPRNPTGKVLKRELRDFEVH---------
MAB_4559|M.abscessus_ATCC_1997 KVPRSFEFVDELPLSGAGKILKRVLRQKYWESRDAGVS--
:.* . * *** . **:*:* : .