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SISLLLEMAASSNAERTAVVSQDVRLTTQELSDLADGGAAVVAGSNAKHLVYVGTGGVLLPVLIFTAARA GVAFTPINYRLSAEGIRALIERLPEPLVVVDARYRDMVGEAPGGVMDSDDFLAAARAAEPAADAFADPDS VAIVLFTSGTTSQPKAVELSHNNLTSYITGTVEFGAADETDAALICVPPYHIAGVSAALSNLYAGRKMVY LPNFDAREWVRLINAENVTTATVVPTMLDRIVTVLENGDPDTGAPIELPSLRNLAYGGSKVGLPLVRRAL ELLPHVGFVNAYGLTETSSTIAVLTPDDHRAALAATTPEAARRLGSVGQAVAGIELQIRDEAGNVLPPGE TGELFVRGEQVSGRYTGIGSVLDEDGWFPTRDIAMLDDEGYLFIGGRSDDTIIRGGENIAPAELEEVLVE HPHVRDVAVVGVEDPQWGQAIVAVVVPAAGVEPDPEELREFVRKSLRGSRTPDEVVFRDELPTTATGKVL RREIIATLAGLRAAPAQQ*
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. avium 104 | MAV_4950 | - | - | 100% (509) | AMP-dependent synthetase and ligase |
| M. avium 104 | MAV_1843 | - | 0.0 | 74.21% (504) | AMP-dependent synthetase and ligase |
| M. avium 104 | MAV_2574 | - | 0.0 | 71.72% (495) | feruloyl-CoA synthetase |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb0276 | fadD2 | 5e-52 | 30.62% (516) | acyl-CoA synthetase |
| M. gilvum PYR-GCK | Mflv_2385 | - | 0.0 | 74.02% (508) | AMP-dependent synthetase and ligase |
| M. tuberculosis H37Rv | Rv0270 | fadD2 | 5e-52 | 30.62% (516) | acyl-CoA synthetase |
| M. leprae Br4923 | MLBr_02546 | fadD2 | 3e-51 | 29.42% (520) | acyl-CoA synthetase |
| M. abscessus ATCC 19977 | MAB_4559 | - | 1e-57 | 33.10% (429) | putative long-chain-fatty-acid CoA ligase |
| M. marinum M | MMAR_0466 | - | 0.0 | 77.78% (504) | long-chain-fatty-acid-CoA ligase |
| M. smegmatis MC2 155 | MSMEG_4883 | - | 0.0 | 74.75% (499) | AMP-dependent synthetase and ligase |
| M. thermoresistible (build 8) | TH_2995 | - | 0.0 | 71.89% (491) | PUTATIVE AMP-dependent synthetase and ligase |
| M. ulcerans Agy99 | MUL_1115 | - | 0.0 | 77.18% (504) | long-chain-fatty-acid-CoA ligase |
| M. vanbaalenii PYR-1 | Mvan_4263 | - | 0.0 | 73.15% (499) | AMP-dependent synthetase and ligase |
CLUSTAL 2.0.9 multiple sequence alignment
MMAR_0466|M.marinum_M --------------------------------------------------
MUL_1115|M.ulcerans_Agy99 --------------------------------------------------
MAV_4950|M.avium_104 --------------------------------------------------
Mflv_2385|M.gilvum_PYR-GCK --------------------------------------------------
Mvan_4263|M.vanbaalenii_PYR-1 --------------------------------------------------
MSMEG_4883|M.smegmatis_MC2_155 --------------------------------------------------
TH_2995|M.thermoresistible__bu --------------------------------------------------
Mb0276|M.bovis_AF2122/97 MPNLTDLPGQAVSKLQKSIGQYVARGTAELHYLRKIIESGAIGLEPPLNY
Rv0270|M.tuberculosis_H37Rv MPNLTDLPGQAVSKLQKSIGQYVARGTAELHYLRKIIESGAIGLEPPLNY
MLBr_02546|M.leprae_Br4923 -----------MTKLR----HYLERGAAEVHYLRSIFESGALGFEPPLTY
MAB_4559|M.abscessus_ATCC_1997 --------------------------------------------------
MMAR_0466|M.marinum_M ----------MSVSLLLEMAASASPDRIAVVSGDLRLTTQQLSDLADGGA
MUL_1115|M.ulcerans_Agy99 ----------MSVSLLLEMAASASPDRIAVVSGDLRLKTQQLSDLADGGA
MAV_4950|M.avium_104 ----------MSISLLLEMAASSNAERTAVVSQDVRLTTQELSDLADGGA
Mflv_2385|M.gilvum_PYR-GCK ----------MSISLLLEMATDAGPDRTAVVSDDTRLTTAELSALADGGA
Mvan_4263|M.vanbaalenii_PYR-1 ----------MSISLLLEMAQDAGPDRTAVVSDDLRLTTAELSALADGGA
MSMEG_4883|M.smegmatis_MC2_155 ----------MSISLLLEMASSGDPDRTAVVSDDTRLTAGELSTLADGAA
TH_2995|M.thermoresistible__bu ------------------MAVSGDPDRVAVIDRDVRLTTTELARLAGGGA
Mb0276|M.bovis_AF2122/97 AALAADIRKWGEVGMLPSHNARRAPNRAAVIDEEGTLTFSELDEAAHAVA
Rv0270|M.tuberculosis_H37Rv AALAADIRKWGEVGMLPSHNARRAPNRAAVIDEEGTLTFSELDEAAHAVA
MLBr_02546|M.leprae_Br4923 AAMAVAMRKWGDLAMLPAMPARRSPHRAALIDEEGTLTYKELDRAAHALA
MAB_4559|M.abscessus_ATCC_1997 ----------MQFTQWLHRALQQYPDRLITIDQGREHTVEQFADRVARLS
..* :. . :: . :
MMAR_0466|M.marinum_M GAIAASDAQ---HVVYVGTGGAMLPLLIFASARAGLPFTSINYRLSADGI
MUL_1115|M.ulcerans_Agy99 GAIAASDAQ---HVVYVGTGGAMLPLLIFASARAGLPFTSINYRLSADGI
MAV_4950|M.avium_104 AVVAGSNAK---HLVYVGTGGVLLPVLIFTAARAGVAFTPINYRLSAEGI
Mflv_2385|M.gilvum_PYR-GCK GVIAASGAR---HVAYVGTGGALLPLLLFASARAAVPVTPLNYRLSAEGL
Mvan_4263|M.vanbaalenii_PYR-1 GVIAASGAR---HVAYVGAGGAMLPLLLFASARAAVPVTPLNYRLSADGL
MSMEG_4883|M.smegmatis_MC2_155 GVIAGSGAA---HVAYVGTGGALLPLLLFASARAAIPFTPLNYRLSAEGL
TH_2995|M.thermoresistible__bu QVIAGSGVQ---HVAYVGLGGAMLPLLIFASTHATVPFTPLNYRLSAEGL
Mb0276|M.bovis_AF2122/97 NGLLAKGVRAGDGVAILARNHRWFVIANYGAARVGARIILLNSEFSGPQI
Rv0270|M.tuberculosis_H37Rv NGLLAKGVRAGDGVAILARNHRWFVIANYGAARVGARIILLNSEFSGPQI
MLBr_02546|M.leprae_Br4923 NGLIAKGVRGGDGIAILARNHRWFVIANYGCARVGARIILLNSEFSGPQI
MAB_4559|M.abscessus_ATCC_1997 AGLRANGVRPGDRVGMLSLNSDRYIEYLTAVPWLGAALNAVNIRWSLAEI
: .... : :. . . . :* . * :
MMAR_0466|M.marinum_M AALIRRLPRPLVVVDERYRDTIAADQS------------------LSSQQ
MUL_1115|M.ulcerans_Agy99 AALIRRLPRPLVVVDERYRETIAADQS------------------LSSQQ
MAV_4950|M.avium_104 RALIERLPEPLVVVDARYRDMVGEAPGGV----------------MDSDD
Mflv_2385|M.gilvum_PYR-GCK RALLDRLPDPLVVVDDEYRDAVGDSYR-T----------------MGSRE
Mvan_4263|M.vanbaalenii_PYR-1 RALLDRLPDPLVVVDDEYRDAVGDGYR-T----------------IGSAE
MSMEG_4883|M.smegmatis_MC2_155 RELVDRLPTPLVIADGEYAGMLAGAGRPV----------------LTSEE
TH_2995|M.thermoresistible__bu HQLIERLPSPLVIADDEYVDAVAGTGKQV----------------MRSSE
Mb0276|M.bovis_AF2122/97 KEVSDREGAKVIIYDDEYTKAVSLAQPPLGKLRALGVNPDDDKPSGSSDE
Rv0270|M.tuberculosis_H37Rv KEVSDREGAKVIIYDDEYTKAVSLAQPPLGKLRALGVNPDDDKPSGSSDE
MLBr_02546|M.leprae_Br4923 KEVSEREGAKVIIYDDEYTKVVSKAEPALGKLRALGVNPDEEEPPGSTDE
MAB_4559|M.abscessus_ATCC_1997 GYSLRESGTRVLLVDDTFKAAAAPLRAACACLETVIYCG-AGPAPQDMIG
. ::: * : .
MMAR_0466|M.marinum_M FLELAGRAEPVA-EFPDPDSVAIVLFTSGTTSQPKAVELTHSNLTSYVTT
MUL_1115|M.ulcerans_Agy99 FLELAGRAEPVA-EFPDPDSVAIVLFTSGTTSQPKAVELTHSNLTSYVTT
MAV_4950|M.avium_104 FLAAARAAEPAADAFADPDSVAIVLFTSGTTSQPKAVELSHNNLTSYITG
Mflv_2385|M.gilvum_PYR-GCK FFEASRTAEPAA-EFADPDSVGVVLFTSGTTSKPKAVELTHTNLTSYIMG
Mvan_4263|M.vanbaalenii_PYR-1 FLDAARTAEPAA-EFADPDAVGVVLFTSGTTSKPKAVELSHANLTSYIMG
MSMEG_4883|M.smegmatis_MC2_155 FLSQARTADPAA-EFADPDAVAVVLFTSGTTSRPKAVELTHNNLTSYITG
TH_2995|M.thermoresistible__bu FVEAARAAEPLE-GFADPDSVGIVLFTSGTTSRPKAVELTHNNLTSYVTG
Mb0276|M.bovis_AF2122/97 TLAELIAHSSTAPAPKASRRASIIILTSGTTGTPKGANRNTPPTLAPIGG
Rv0270|M.tuberculosis_H37Rv TLAELIAHSSTAPAPKASRRASIIILTSGTTGTPKGANRNTPPTLAPIGG
MLBr_02546|M.leprae_Br4923 TLAELIARSSSAPAPKVRKRASIIILTSGTTGTPKGANRNTPASLAPLGG
MAB_4559|M.abscessus_ATCC_1997 YEDLLASHEPIDDTRTGGDSLLGVFYTGGTTGNPKGVMLSHNNVLASAMG
.. :: *.***. **.. . :
MMAR_0466|M.marinum_M TVEFGCAAP-SDAALICVPPYHIAGVSAVLSNLYAGRKMVYLTNFDAHEW
MUL_1115|M.ulcerans_Agy99 TVEFGCAAP-SDAALICVPPYHIAGVSAVLSNLYAGRKMVYLNNFDAHEW
MAV_4950|M.avium_104 TVEFGAADE-TDAALICVPPYHIAGVSAALSNLYAGRKMVYLPNFDAREW
Mflv_2385|M.gilvum_PYR-GCK TVEFASAEP-DDAALICVPPYHIAGVSAALSNLYAGRKMVYLRHFDAEKW
Mvan_4263|M.vanbaalenii_PYR-1 TVEFASAEP-DDAALICVPPYHIAGVSAALSNLYAGRKMVYLTQFDAGRW
MSMEG_4883|M.smegmatis_MC2_155 TVEFGSAAP-EDAALICVPPYHIAGVSAAMSNLYAGRKMVYLRNFDAHRW
TH_2995|M.thermoresistible__bu TVEFADADA-EDAALVCVPPYHIAGVGAALSNLYAGRKIVCLSRFDPAEW
Mb0276|M.bovis_AF2122/97 ILSHVPFKA-GEVTLLPSPMFHALGYMHAALAMFLGSTLVLRRRFKPALV
Rv0270|M.tuberculosis_H37Rv ILSHVPFKA-GEVTLLPSPMFHALGYMHAALAMFLGSTLVLRRRFKPALV
MLBr_02546|M.leprae_Br4923 ILSKVPFRA-HEVTLLPAPMFHALGYLHATLAMFLGSTLVLRRRFKPATV
MAB_4559|M.abscessus_ATCC_1997 SLSTGNFLTRGGRLLHSAPMFHMADFSAVLAGNLSGSTHVIVPSFSPQGV
:. * * :* . . * . * *..
MMAR_0466|M.marinum_M VRLTNAEQVTTATVVPTMLDRIVAVLETGEH------KLPSLRNLAYGGS
MUL_1115|M.ulcerans_Agy99 VRLTNAEQVTTATVVPTMLDRIVAVLETGEH------KLPSLRNLAYGCS
MAV_4950|M.avium_104 VRLINAENVTTATVVPTMLDRIVTVLENGDPDTGAPIELPSLRNLAYGGS
Mflv_2385|M.gilvum_PYR-GCK VRLVADEGVTSATVVPTMLDRIVTVLETTDT------ALPTLRTLAYGGS
Mvan_4263|M.vanbaalenii_PYR-1 VDLVTGEGVTSATVVPTMLDRIVSVLETRGT------TLPTLRTLAYGGS
MSMEG_4883|M.smegmatis_MC2_155 VELVRTEGVTSATVVPTMLDRIVTALETTRA------ELPTLRNLAYGGS
TH_2995|M.thermoresistible__bu VRLVADEKVTTATVVPTMLDRIVTELEARPR------ELPSLRNLAYGGS
Mb0276|M.bovis_AF2122/97 LEDIEKHKATSMVVVPVMLSRILDQLEKTEPKP----DLSSLKIVFVSGS
Rv0270|M.tuberculosis_H37Rv LEDIEKHKATSMVVVPVMLSRILDQLEKTEPKP----DLSSLKIVFVSGS
MLBr_02546|M.leprae_Br4923 LEDIEKHQATAMVVVPVMLSRILDTLENLETKS----DLSSLRVVFVSGS
MAB_4559|M.abscessus_ATCC_1997 LDAIVAHDVQDMLLVPTMIQMLVDHPGAAQL------DLSGIRSITYGAS
: . . :**.*:. :: *. :: : . *
MMAR_0466|M.marinum_M KVGLPLVRRALELLPDVGFVNAYGLTETSSTIAVLTPDDHRAAQSAAAAG
MUL_1115|M.ulcerans_Agy99 KVGLPLVRRALELLPDVGFVNAYRLTETSSTIAVLTPDDHRAAQSAAAAG
MAV_4950|M.avium_104 KVGLPLVRRALELLPHVGFVNAYGLTETSSTIAVLTPDDHRAALAATTPE
Mflv_2385|M.gilvum_PYR-GCK KVPLPLVRKALSLLPDVGFVNAYGLTETSSTIAVLTPDDHRAALAAADDA
Mvan_4263|M.vanbaalenii_PYR-1 KVPLPLVRKALSLLPAVGFVNAYGLTETSSTIAVLTPDDHRAALAASDEA
MSMEG_4883|M.smegmatis_MC2_155 KVALPLVRKALELMPNVGFVNAYGLTETSSTIAVLGPDDHRAALASDDPG
TH_2995|M.thermoresistible__bu KVGLPLVRKALELLPDVGFVNAYGLTETSSTIAVLGPEDHRIALASDDEK
Mb0276|M.bovis_AF2122/97 QLGAELATRALGDLGPVIYN-------------MYGSTEVAFATIAGPKD
Rv0270|M.tuberculosis_H37Rv QLGAELATRALGDLGPVIYN-------------MYGSTEVAFATIAGPKD
MLBr_02546|M.leprae_Br4923 QLGAELATRALEELGPVIYN-------------MYGSTEIAFATIAGPKD
MAB_4559|M.abscessus_ATCC_1997 VISEAVLQRATRVFPNARFTQAYG---------MTEVSPVATLLLPDDHD
: : :* : . : : .
MMAR_0466|M.marinum_M LARRLGSVGRPVPGVELQIRGQDGRVLGPGETGELFVRGGQVSGRYTGI-
MUL_1115|M.ulcerans_Agy99 LARRLGSVGRPVPGVELQIRGQDGRVLGPGETGELFVRGEQVSGRYTGI-
MAV_4950|M.avium_104 AARRLGSVGQAVAGIELQIRDEAGNVLPPGETGELFVRGEQVSGRYTGI-
Mflv_2385|M.gilvum_PYR-GCK ALRRLGSVGQPVPGIEVEVRAEDGTVLGPGETGELFVRGEQVSGKYTDI-
Mvan_4263|M.vanbaalenii_PYR-1 AIRRLGSVGQPVPGIEVEIRADDGTVLGAGETGELFVRGEQVSGKYTDI-
MSMEG_4883|M.smegmatis_MC2_155 VTRRLGSVGQVVPGIEVQIRGEDGTVLGPGETGELFVRGEQVSGRYTEI-
TH_2995|M.thermoresistible__bu VVRRLGSVGQPVPGIEIQIRSKNGEVLGPGETGELFVRGPQVSGRYTGI-
Mb0276|M.bovis_AF2122/97 LQFNPSTVGPVVKGVTVKILDENGNEVPQGAVGRIFVGNAFPFEGYTGG-
Rv0270|M.tuberculosis_H37Rv LQFNPSTVGPVVKGVTVKILDENGNEVPQGAVGRIFVGNAFPFEGYTGG-
MLBr_02546|M.leprae_Br4923 LQINSATVGPVVKGVKVKILDDSGKEVPRGQVGRIFVGNAFPFEGYTGG-
MAB_4559|M.abscessus_ATCC_1997 DPALLRSAGRAAPHCEVRIVDPDDNEVPRGEIGEVIVKGDNVMLGYWELP
:.* . :.: . : * *.::* . *
MMAR_0466|M.marinum_M --GSVLDGHGWFGTKDIAMLDEDGYLFIGGRSDDTIIRGGENIAPAELEE
MUL_1115|M.ulcerans_Agy99 --GSVLDGHGWFGTKDIAMLDEDGYLFIGGRSDDTIIRGGENIAPAELEE
MAV_4950|M.avium_104 --GSVLDEDGWFPTRDIAMLDDEGYLFIGGRSDDTIIRGGENIAPAELEE
Mflv_2385|M.gilvum_PYR-GCK --GSVLDENGWFPTKDVAYLDSEGYLFIGGRSDDTIIRGGENIAPAEIED
Mvan_4263|M.vanbaalenii_PYR-1 --GSVLDEQGWFPTKDVAFLDEEGYLFIGGRSDDTIIRGGENIAPAEIED
MSMEG_4883|M.smegmatis_MC2_155 --GSVLDEDGWFPTKDVAMLDQDGYLFIGGRSDDTIIRGGENIAPAEIED
TH_2995|M.thermoresistible__bu --GSVLDDDGWFPTKDVAMVDEDGYLFIGGRSDDTIIRGGENIAPAEIEN
Mb0276|M.bovis_AF2122/97 --GGKQIIDGLLSSGDVGYFDERGLLYVSGRDDEMIVSGGENVFPAEVED
Rv0270|M.tuberculosis_H37Rv --GGKQIIDGLLSSGDVGYFDERGLLYVSGRDDEMIVSGGENVFPAEVED
MLBr_02546|M.leprae_Br4923 --GGKQIIDGLLSSGDVGYFDEHDLLYISGRDDEMIVSGGENVFPAEVED
MAB_4559|M.abscessus_ATCC_1997 EESAAAIRDGWMHTGDAGRMDDRGYVYIVDRIKDMVVTGGENVYSAEVEN
.. .* : : * . .*. . ::: .* .: :: ****: .**:*:
MMAR_0466|M.marinum_M VLIEHPQVRDVAVVGVEDAQWGQEIVAVVVPVAGVDPDPEELREFVRRSL
MUL_1115|M.ulcerans_Agy99 VLIEHPQVRDVAVVGVEDAQWGQEIVAVVVPVAGVDPDPEELREFVRRSL
MAV_4950|M.avium_104 VLVEHPHVRDVAVVGVEDPQWGQAIVAVVVPAAGVEPDPEELREFVRKSL
Mflv_2385|M.gilvum_PYR-GCK VLVEHPHVRDCAVVGADDPQWGQIIVAVVVPHQDTAPDPEDLRAHVRSQL
Mvan_4263|M.vanbaalenii_PYR-1 VLVEHPHVRDCAVVGADDPEWGQIIVAVVVAQPGTEPDVEDLRSHVRSQL
MSMEG_4883|M.smegmatis_MC2_155 VLVEHPDVRDVAVVGPEDPQWGQIIVAVVVPADGAEPDADVLREHVRKHL
TH_2995|M.thermoresistible__bu VLVEHPQVREAVVVGPEDPQWGQILVAVVVPAEGETPDPEELRAHVRKHL
Mb0276|M.bovis_AF2122/97 LISGHPDVVEAAAIGVDDKEFGARLRAFVVKKPGADLDEDTIKQYVRDHL
Rv0270|M.tuberculosis_H37Rv LISGHPDVVEAAAIGVDDKEFGARLRAFVVKKPGADLDEDTIKQYVRDHL
MLBr_02546|M.leprae_Br4923 LISGHPEVVEATAIGVDDKEWGARLRAFVVKKQDATIDEDAIKAYVRDHL
MAB_4559|M.abscessus_ATCC_1997 ALAKHPAVAACAVIGIPDEQWGERVHAVVVKQAQSECCDGDLQEHCREHI
: ** * ..:* * ::* : *.** :: . * :
MMAR_0466|M.marinum_M RGSRTPDRVVFRHELPTTATGKILRREIIEELAGTKS------
MUL_1115|M.ulcerans_Agy99 RGSRTPDRVVFRRELPTTATGKILRREIIEELAGTKS------
MAV_4950|M.avium_104 RGSRTPDEVVFRDELPTTATGKVLRREIIATLAGLRAAPAQQ-
Mflv_2385|M.gilvum_PYR-GCK RGSRTPDRVVFRDELPTNATGKVLRRELVSELNQDRSPAQPAV
Mvan_4263|M.vanbaalenii_PYR-1 RGSRTPDRVVFRDELPTNATGKVLRRELVDELNQDPSPAQPAV
MSMEG_4883|M.smegmatis_MC2_155 RGSRTPDRVVFRAELPTNATGKVLRRQLVDEL-------QPIS
TH_2995|M.thermoresistible__bu RGSRTPDKVVFRTELPVNALGKVLRRQLVDELQT----KEPA-
Mb0276|M.bovis_AF2122/97 ARYKVPREVIFLDELPRNPTGKVLKRELRKL------------
Rv0270|M.tuberculosis_H37Rv ARYKVPREVIFLDELPRNPTGKVLKRELRKL------------
MLBr_02546|M.leprae_Br4923 ARYKVPREVIFLEELPRNPTGKVLKRELRDFEVH---------
MAB_4559|M.abscessus_ATCC_1997 ANYKVPRSFEFVDELPLSGAGKILKRVLRQKYWESRDAGVS--
:.* . * *** . **:*:* :