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SRGKRANPASGSGSRGTLLGMAELTEPRFLDERLAHWATVNPDGEAMDYLERSWTWSQWNDRVRRLAGAL TERGISRGDVVAFLDKNHPACVELTFAAASLGAATAIVNCRLAPDELDYVLNDSGAKLLIVGEEFTGSID KIRDKLGRVEHVISVKPDGDDEYEAMLEASSPVDRADDVEPGDVCIIMYSSGTTGRPKGVELTQANIIAH TVNAHEGFEFDEGDKNMVSMPLFHVGGSSYVQFGIHDGFPSVMTRDVDGMTLAGAILKGANRTFLVPAVL AKVLDAGEDAVKLFGSLKTFAYGASPMPLPLLRQALEAWPNTDFMQAYGLTELCGVISHLLPEAHRDPGR EERLSSAGTLVPNAELRVVDPDTLKDVPTGEQGELWFRSPQLMKGYHNKPEATAESITEDGWFRTGDVGR VDDGGYIFVEDRLKDMIISGGENIYSIEVERVLAEHDAVVEVAVIGVPDDKWGEVVKAVVVIEGETSESE LIAFARERLAAYKCPKSVDITDELPRKPTGKILKKELRKPHWDGRDRATV*
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. gilvum PYR-GCK | Mflv_5271 | - | - | 100% (541) | AMP-dependent synthetase and ligase |
| M. gilvum PYR-GCK | Mflv_0027 | - | e-106 | 42.77% (498) | AMP-dependent synthetase and ligase |
| M. gilvum PYR-GCK | Mflv_0714 | - | 2e-81 | 37.34% (533) | acyl-CoA synthetase |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb0172 | fadD5 | 7e-80 | 37.30% (504) | acyl-CoA synthetase |
| M. tuberculosis H37Rv | Rv0166 | fadD5 | 7e-80 | 37.50% (504) | acyl-CoA synthetase |
| M. leprae Br4923 | MLBr_02546 | fadD2 | 1e-48 | 29.54% (501) | acyl-CoA synthetase |
| M. abscessus ATCC 19977 | MAB_4569c | - | 3e-86 | 40.08% (509) | fatty-acid-CoA ligase FadD |
| M. marinum M | MMAR_0409 | fadD5 | 1e-83 | 38.69% (504) | fatty-acid-CoA ligase FadD5 |
| M. avium 104 | MAV_3523 | - | 1e-152 | 52.02% (496) | acyl-CoA synthase |
| M. smegmatis MC2 155 | MSMEG_0131 | - | 6e-89 | 40.62% (512) | acyl-CoA synthetase |
| M. thermoresistible (build 8) | TH_2878 | - | 6e-87 | 40.58% (515) | PUTATIVE AMP-binding enzyme, putative |
| M. ulcerans Agy99 | MUL_0650 | fadD8 | 5e-63 | 32.67% (502) | acyl-CoA synthetase |
| M. vanbaalenii PYR-1 | Mvan_0970 | - | 0.0 | 92.12% (520) | AMP-dependent synthetase and ligase |
CLUSTAL 2.0.9 multiple sequence alignment
Mflv_5271|M.gilvum_PYR-GCK -----------------MSRGKRANPASGSGSRGTLLGMAELTEPRFLDE
Mvan_0970|M.vanbaalenii_PYR-1 --------------------------------------MADLPEPRFLDE
MAV_3523|M.avium_104 --------------------------------------MPAAETRETLAG
MSMEG_0131|M.smegmatis_MC2_155 -----------------------------MTAEPSQIEQPYLARRQNWVN
TH_2878|M.thermoresistible__bu ------------------------------------LQQPYLARRQNWVN
MAB_4569c|M.abscessus_ATCC_199 -----------------------MTDTIDATVQSAGLAQPYRARRQNWCN
Mb0172|M.bovis_AF2122/97 --------------------MTAQLASHLTRALTLAQQQPYLARRQNWVN
Rv0166|M.tuberculosis_H37Rv --------------------MTAQLASHLTRALTLAQQQPYLARRQNWVN
MMAR_0409|M.marinum_M --------------------MTAELASHLPQAA-HALEQPYLARRQNWVN
MUL_0650|M.ulcerans_Agy99 ------------------------------------MSDDLLRNPTHNGH
MLBr_02546|M.leprae_Br4923 MTKLRHYLERGAAEVHYLRSIFESGALGFEPPLTYAAMAVAMRKWGDLAM
Mflv_5271|M.gilvum_PYR-GCK RLAHWATVNPDGEAMDYLERSWTWSQWNDRVRRLAGALTERGISRGDVVA
Mvan_0970|M.vanbaalenii_PYR-1 RIAHWAQVKPDDEAMDYLDRSWTWSQWNDRVRRLAGALTDRGVKRGDVVA
MAV_3523|M.avium_104 IVERHAQRRPDAIAIRYGERQWSWAEWSSRIRRAAGALRGDGIERGQCVA
MSMEG_0131|M.smegmatis_MC2_155 QLTRHAMMQPDKTALRFLGRTTTWRELDDRVTRLAGALNRRGVGFGDRVL
TH_2878|M.thermoresistible__bu HLARHALMQPDATALRFMGQTTTWADLHHRVNRLAGALRRRGVEFGDRVL
MAB_4569c|M.abscessus_ATCC_199 QLARHALMQPDATAIRYLGHSISWAELHQRVQALADALSRRGVGFGDRVL
Mb0172|M.bovis_AF2122/97 QLERHAMMQPDAPALRFVGNTMTWADLRRRVAALAGALSGRGVGFGDRVM
Rv0166|M.tuberculosis_H37Rv QLERHAMMQPDAPALRFVGNTMTWADLRRRVAALAGALSGRGVGFGDRVM
MMAR_0409|M.marinum_M QLERHAMMQPRATAIRYLGHTVTWADLRYRVAALAGSLSRRGVGFGDRVM
MUL_0650|M.ulcerans_Agy99 LLVGALERHKNRPVLFLGDTTLTGGQLAERISQYIQAFEALGAGTGVTVG
MLBr_02546|M.leprae_Br4923 LPAMPARRSPHRAALIDEEGTLTYKELDRAAHALANGLIAKGVRGGDGIA
.: : : .: * * :
Mflv_5271|M.gilvum_PYR-GCK FLDKNHPACVELTFAAASLGAATAIVNCRLAPDELDYVLNDSGAKLLIVG
Mvan_0970|M.vanbaalenii_PYR-1 FLDKNHPACVELTFAAASLGAANAIINFRLAADELDYVLNDSGAKLLIVG
MAV_3523|M.avium_104 FLDKNHPACLEVLIGGASVGAVTTVVNWRVIGDELVHVLADSGARVLVVG
MSMEG_0131|M.smegmatis_MC2_155 ILMLNRTEFVEAMLAANRLGAIAVPVNFRMTPPEIAYLVSDCEAHVVVTE
TH_2878|M.thermoresistible__bu ILMLNRTEFIETMLAANRLGAIAVPVNFRMTPPEIAFLAKDCEAKVIATE
MAB_4569c|M.abscessus_ATCC_199 ILMLNRPEYVESWLAINELGAIAIPVNFRMTPPEVAFLVENSGAKVAITE
Mb0172|M.bovis_AF2122/97 ILMLNRTEFVESVLAANMIGAIAVPLNFRLTPTEIAVLVEDCAAHVMLTE
Rv0166|M.tuberculosis_H37Rv ILMLNRTEFVESVLAANMIGAIAVPLNFRLTPTEIAVLVEDCVAHVMLTE
MMAR_0409|M.marinum_M ILMLNRTEFVESVLAANMLGAIAVPLNFRLTPSEIAFLVEDCAPRLVITE
MUL_0650|M.ulcerans_Agy99 LLSLNRPEVLMILGASQARGYRRTALHPLGSLDDHAYVLYDAGASALIID
MLBr_02546|M.leprae_Br4923 ILARNHRWFVIANYGCARVGARIILLNSEFSGPQIKEVSEREGAKVIIYD
:* *: : . * :: : : .
Mflv_5271|M.gilvum_PYR-GCK E--EFTGSIDKIRDKLGRVEHVISVKPDGDDE------YEAMLEASSP-V
Mvan_0970|M.vanbaalenii_PYR-1 E--EFKPSIDKIRDRLEHIEHVISVKPEGDDE------YEAMLEASSP-V
MAV_3523|M.avium_104 A--ELRPAAEAAARRVPSLERIIEVG----DE------YESLLAAAEP-A
MSMEG_0131|M.smegmatis_MC2_155 P--VLAGVATAVRDLDATLQTVIVAGGATEDG---VLGYDDLIAEEGE-P
TH_2878|M.thermoresistible__bu P--VLAGVATAVRDLAPSLSSVIVAGAADGSDTPDVLDYEALIAEPGE-D
MAB_4569c|M.abscessus_ATCC_199 T--VLAPVAAAVRQQAPALETVIVAGSASENG---VLGYEEVIAEPGE-P
Mb0172|M.bovis_AF2122/97 A--ALAPVAIGVRNIQPLLSVIVVAGGSSQDS---VFGYEDLLNEAGD-V
Rv0166|M.tuberculosis_H37Rv A--ALAPVAIGVRNIQPLLSVIVVAGGSSQDS---VFGYEDLLNEAGD-V
MMAR_0409|M.marinum_M E--VLAQVAVGVREIAPALSTIVVAGGASDPT---VVGYDELISETGD-P
MUL_0650|M.ulcerans_Agy99 PNPMFVERALGLLEKVDSLEQVLTIGPVPEALEDRAVDLSAEAAKYQPKP
MLBr_02546|M.leprae_Br4923 D-----EYTKVVSKAEPALGKLRALGVNPDEEEPPGSTDETLAELIARSS
: : .
Mflv_5271|M.gilvum_PYR-GCK DRADDVEPGDVCIIMYSSGTTGRPKGVELTQANIIAHTVNAHEGFEFDE-
Mvan_0970|M.vanbaalenii_PYR-1 GRSADVEPGDVCIIMYSSGTTGRPKGVELTQANIIAHTVNAHEGFEFDE-
MAV_3523|M.avium_104 PSDAGVDTDETALVIYSSGTTGRPKGVLLSQRALVNHAANLAPAFPFGD-
MSMEG_0131|M.smegmatis_MC2_155 PAPVDIPNDSPALIMYTSGTTGRPKGAVLTHTNLSAQAMMHLFTTGADLN
TH_2878|M.thermoresistible__bu PPPVDIPDDSPALIMYTSGTTGNPKGAVLTHSNLTGQAMTNLFTIGVDLA
MAB_4569c|M.abscessus_ATCC_199 HAEVDLPGDTPALIMYTSGTTGRPKGAVLTHLNLSGQAMTYLLSTTVDLN
Mb0172|M.bovis_AF2122/97 HEPVDIPNDSPALIMYTAGTTGRPKGAVLTHANLTGQAMTALYTSGANIN
Rv0166|M.tuberculosis_H37Rv HEPVDIPNDSPALIMYTSGTTGRPKGAVLTHANLTGQAMTALYTSGANIN
MMAR_0409|M.marinum_M PEPVDIPNDSPALIMYTSGTTGRPKGAVLTHTNLTGQVMTALYTGGANIN
MUL_0650|M.ulcerans_Agy99 LVVADLPPNHIGGLTYTGGTTGKPKGVIGTAGNIT--AMTSIQLAEWEWP
MLBr_02546|M.leprae_Br4923 SAPAPKVRKRASIIILTSGTTGTPKGANRNTPASLAPLGGILSKVPFRA-
: :.**** ***. .
Mflv_5271|M.gilvum_PYR-GCK GDKNMVSMPLFHVGGSS-YVQFGIHDGFPSVMT--RDVDGMTLAGAILKG
Mvan_0970|M.vanbaalenii_PYR-1 GDKNMVSMPLFHVGGSS-YVQFGIHDGVPSVMT--RDVDGMTLAGAILKG
MAV_3523|M.avium_104 GDANLVAMPLFHVGGIG-YALFGIRAGAPTIMT--REPDAAALIGAVRAG
MSMEG_0131|M.smegmatis_MC2_155 SDVGFVGVPLFHIAGVAGSVLSGVTLGVPTVLYPLGAFDPDGLLDVLESE
TH_2878|M.thermoresistible__bu NDVGFIGVPLFHIAGIAGNVLSGLTLGLPTVLYPLGAFDPGGLLDVLEAE
MAB_4569c|M.abscessus_ATCC_199 SDVGFIGVPMFHIAGVG-NMITGLLLGLPTVIHPLGAFDPGALLDVLESE
Mb0172|M.bovis_AF2122/97 SDVGFVGVPLFHIAGIG-NMLTGLLLGLPTVIYPLGAFDPGQLLDVLEAE
Rv0166|M.tuberculosis_H37Rv SDVGFVGVPLFHIAGIG-NMLTGLLLGLPTVIYPLGAFDPGQLLDVLEAE
MMAR_0409|M.marinum_M SDVGFIGVPFFHIAGIG-NMLSGMLLGVPTVIYPLGAFNPGQLLDVLEAE
MUL_0650|M.ulcerans_Agy99 EHPRFLMCTPLSHAGAAFFTPTLVKGGE---MIVLAKFDPGEVLRVIEEQ
MLBr_02546|M.leprae_Br4923 HEVTLLPAPMFHALGYL-HATLAMFLGSTLVLR--RRFKPATVLEDIEKH
. :: . : * : * : . : :
Mflv_5271|M.gilvum_PYR-GCK ANRTFLVPAVLAKVLDAGED--AVKLFGS-LKTFAYGASPMPLPLLRQAL
Mvan_0970|M.vanbaalenii_PYR-1 ANRTFLVPAVLAKVLEAGED--AVKLFGS-LKTFAYGASPMPLPLLRQAL
MAV_3523|M.avium_104 ATHAFFVPPVIARFLDAGEA--ARASIAG-LRYIVYGAAPMPLPLLHRAL
MSMEG_0131|M.smegmatis_MC2_155 QVTGIFLVPAQWQAVCAAQR--ARPRNLK-LRALSWGAAPASDTLLREMN
TH_2878|M.thermoresistible__bu KVTGIFLVPAQWQAVCAEQR--ARPRDLR-LRALSWGAAPASDTLLQEMT
MAB_4569c|M.abscessus_ATCC_199 GVTGIFLVPAQWQAVCAAQQ--ANPRKVR-LKTLSWGAAPATDVLLRTMS
Mb0172|M.bovis_AF2122/97 KVTGIFLVPAQWQAVCTEQQ--ARPRDLR-LRVLSWGAAPAPDALLRQMS
Rv0166|M.tuberculosis_H37Rv KVTGIFLVPAQWQAVCTEQQ--ARPRDLR-LRVLSWGAAPAPDALLRQMS
MMAR_0409|M.marinum_M RVSGIFLVPAQWQAVCAEQQ--ARPRELS-LRVMSWGAAPAPDALLRQMS
MUL_0650|M.ulcerans_Agy99 RITATMLVPSMLYALMDHPD--PHTRDLSSLETVYYGASAINPVRLAEAI
MLBr_02546|M.leprae_Br4923 QATAMVVVPVMLSRILDTLENLETKSDLSSLRVVFVSGSQLGAELATRAL
.: . : *. . ..:
Mflv_5271|M.gilvum_PYR-GCK EAWPNTDFMQAYGLTELCGVISHLLPEAHRDPGREERLSSAGTLVPNAEL
Mvan_0970|M.vanbaalenii_PYR-1 QAWPNTDFMQAYGLTELCGVISHLLPEAHRDPGREERLSSAGTLVPNAEL
MAV_3523|M.avium_104 STWPGTKFVQVYGQTELCGAVTALSDDDHRDAARPQLQLSAGKAVQGCEI
MSMEG_0131|M.smegmatis_MC2_155 EVFPDAQIFAAFGQTEMSPVTCMLLGDDAIR-----KLGSVGRVIPTVSA
TH_2878|M.thermoresistible__bu EVFPGTKIFAAFGQTEMSPVTCMLLAEDAVR-----KLGSVGKVIPTVAA
MAB_4569c|M.abscessus_ATCC_199 ETFPDARILAAFGQTEMSPVTCMLMGDDAIR-----KMGSVGRVIPTVSA
Mb0172|M.bovis_AF2122/97 ATFPETQILAAFGQTEMSPVTCMLLGEDAIA-----KRGSVGRVIPTVAA
Rv0166|M.tuberculosis_H37Rv ATFPETQILAAFGQTEMSPVTCMLLGEDAIA-----KRGSVGRVIPTVAA
MMAR_0409|M.marinum_M EVFPGTQIMAAFGQTEMSPVTCMLLGEDAIR-----KRGSVGKVIPTVSA
MUL_0650|M.ulcerans_Agy99 DRFG-PIFAQYYGQSEAPMVITYLPKADHDE----KRLTSCGRPTLFARV
MLBr_02546|M.leprae_Br4923 EELG-PVIYNMYGSTEIAFATIAGPKDLQIN------SATVGPVVKGVKV
. : :* :* . : *
Mflv_5271|M.gilvum_PYR-GCK RVVDPDTLKDVPTGEQGELWFRSPQLMKGYHNKPEATAESITEDGWFRTG
Mvan_0970|M.vanbaalenii_PYR-1 RVVDPDTLEDVPTGEQGELWFRSPQLMKGYHNKPEATAEAITEDGWFRTG
MAV_3523|M.avium_104 RIVDPNNCAELPAGRSGEVWVRSNQNMSGYLNRAEATAETITADGWVRTG
MSMEG_0131|M.smegmatis_MC2_155 RVVD-DNMNDVPVGEVGEIVYRGPNLMVGYWNNPKATAEAFAG-GWFHSG
TH_2878|M.thermoresistible__bu RIVD-DTMNDVPVGEVGEIVYRAPTLMAGYWNNPQATAEAFAG-GWFHSG
MAB_4569c|M.abscessus_ATCC_199 RVVD-DNMNDVPVGEVGEIVYRAPTLMQGYWNNPEATADAFAG-GWFHSG
Mb0172|M.bovis_AF2122/97 RVVD-QNMNDVPVGEVGEIVYRAPTLMSCYWNNPEATAEAFAG-GWFHSG
Rv0166|M.tuberculosis_H37Rv RVVD-QNMNDVPVGEVGEIVYRAPTLMSCYWNNPEATAEAFAG-GWFHSG
MMAR_0409|M.marinum_M RVVD-DEMNDVPIGQVGEIVYRAPTLMSGYWNNPDATAEAFAG-GWFHSG
MUL_0650|M.ulcerans_Agy99 ALLG-EDGKPVPQGEPGEICVSGPLLAGGYWNKPDDTAQTFRD-GWLHTG
MLBr_02546|M.leprae_Br4923 KILD-DSGKEVPRGQVGRIFVGNAFPFEGYTG---GGGKQIID-GLLSSG
::. : :* *. *.: * . .. : * . :*
Mflv_5271|M.gilvum_PYR-GCK DVGRVDDGGYIFVEDRLKDMIISGGENIYSIEVERVLAEHDAVVEVAVIG
Mvan_0970|M.vanbaalenii_PYR-1 DVGRVDDGGYIFVEDRLKDMIISGGENIYSIEVERVLAEHEAVVEVAVIG
MAV_3523|M.avium_104 DVGRLDADGYVYIEDRLKDMIITGGENVYGPEVESVLIEHPAVVDAAVIG
MSMEG_0131|M.smegmatis_MC2_155 DLVRQDEEGYVWVVDRKKDMIISGGENIYCAEVENVLAAHPAIMEVAVIG
TH_2878|M.thermoresistible__bu DLVRQDEEGYIWVVDRKKDMIISGGENIYCAEVENVLAAHPAVAEVAVVG
MAB_4569c|M.abscessus_ATCC_199 DLVRQDEDGFVWVVDRKKDMIISGGENIYCAEVENALAEHEQIAEVAVIG
Mb0172|M.bovis_AF2122/97 DLVRMDSDGYVWVVDRKKDMIISGGENIYCAELENVLASHPDIAEVAVIG
Rv0166|M.tuberculosis_H37Rv DLVRMDSDGYVWVVDRKKDMIISGGENIYCAELENVLASHPDIAEVAVIG
MMAR_0409|M.marinum_M DLVRMDEDGYVWVVDRKKDMIISGGENVYCAEVENVLASHPSIVEVAVIG
MUL_0650|M.ulcerans_Agy99 DMAREDEDGFYFIVDRVKDMIVTGGFNVFPREVEDVVAEHSAVAQVCVVG
MLBr_02546|M.leprae_Br4923 DVGYFDEHDLLYISGRDDEMIVSGGENVFPAEVEDLISGHPEVVEATAIG
*: * . :: .* .:**::** *:: *:* : * : :. .:*
Mflv_5271|M.gilvum_PYR-GCK VPDDKWGEVVKAVVVIEG---------ETSESELIAFARERLAAYKCPKS
Mvan_0970|M.vanbaalenii_PYR-1 IPDDRWGEVVKAVVVLER---------EVTETELIAFARERLAAYKCPKT
MAV_3523|M.avium_104 VPDDFWGESVKAIVVADG---------DVDAADVIEFCRRHLAGFKCPRT
MSMEG_0131|M.smegmatis_MC2_155 RPHEKWGEVPVAVVALNSA-----TAESISLGELDSFLTERLARYKHPKA
TH_2878|M.thermoresistible__bu RPHEKWGEVPVAVVALN-------PDAELTMAELEEFLADRLARYKHPKV
MAB_4569c|M.abscessus_ATCC_199 RPHEKWGEVPVAVAAIH--------GAALTLAELDGFLTARLARYKHPKD
Mb0172|M.bovis_AF2122/97 RADEKWGEVPIAVAAVT--------NDDLRIEDLGEFLTDRLARYKHPKA
Rv0166|M.tuberculosis_H37Rv RADEKWGEVPIAVAAVT--------NDDLRIEDLGEFLTDRLARYKHPKA
MMAR_0409|M.marinum_M RADEKWGEVPIAVAAVT--------KDHLRIEDLDEYLTERLARYKHPKA
MUL_0650|M.ulcerans_Agy99 APDEKWGEAVTAVVVLRADAARDDAAIETMTAEIQAAVKQRKGSVQSPKQ
MLBr_02546|M.leprae_Br4923 VDDKEWGARLRAFVVKKQ-------DATIDEDAIKAYVRDHLARYKVPRE
.. ** *... : : . : *:
Mflv_5271|M.gilvum_PYR-GCK VDITDELPRKPTGKILKKELRKPHWDGRDRATV----------------
Mvan_0970|M.vanbaalenii_PYR-1 VDITDELPRNPTGKILKKELRKPHWEGRDRANV----------------
MAV_3523|M.avium_104 VDFVAELPRNASGKILKTQLREPFWRDRDRRV-----------------
MSMEG_0131|M.smegmatis_MC2_155 LEVVDALPRNPSGKVLKTELRERFGRATSLDGGESNSATTVSAG-----
TH_2878|M.thermoresistible__bu LEIVDALPRNPAGKVLKTELRARFAAGPSAEKEQSSSGATTSAGSS---
MAB_4569c|M.abscessus_ATCC_199 LVIVDALPRNPSGKVLKTELRQRFGIAD---------------------
Mb0172|M.bovis_AF2122/97 LEIVDALPRNPAGKVLKTELRLRYGACVNVERRSASAGFTERRENRQKL
Rv0166|M.tuberculosis_H37Rv LEIVDALPRNPAGKVLKTELRLRYGACVNVERRSASAGFTERRENRQKL
MMAR_0409|M.marinum_M LEIVDALPRNPSGKVLKTELRLRYGVGVYSESRSVSRSFEFREGS----
MUL_0650|M.ulcerans_Agy99 VVVVDSLPLTGLGKPDKKAVRARFWEGAGRAVG----------------
MLBr_02546|M.leprae_Br4923 VIFLEELPRNPTGKVLKRELRDFEVH-----------------------
: . ** . ** * :*