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PGNDVRCVVTGATGYIGGRLVPELLARGLQVRAMARTPSKLDRTEWRDRVEVVRGDLTEADSLTAAFNGA DVVYYLVHSMGTSRDFVAEERRSAHNVVEAAKKAGVRRIVYLSGLHPDRKELSRHLASRVEVGEILMQSG IETVVLQAGIVIGSGSASFEMVRHLTDRLPVMTTPKWVHNKIQPISISDVLHYLAEAATAEFGESRTWDI GGPDVMEYGDAMQGYADVAGLRRRVMVVLPFLTPTIASWWVGLVTPIPSGLARPLVESLECDAICHEHDI DAVIPPPKGGLIGYREAVAKELRSEGGSSAGRRHLLRFSPAASD*
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. gilvum PYR-GCK | Mflv_1635 | - | - | 100% (325) | NAD-dependent epimerase/dehydratase |
| M. gilvum PYR-GCK | Mflv_2122 | - | 6e-57 | 44.33% (300) | NAD-dependent epimerase/dehydratase |
| M. gilvum PYR-GCK | Mflv_2075 | - | 3e-15 | 41.53% (118) | 3-beta hydroxysteroid dehydrogenase/isomerase |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb1136c | - | 2e-12 | 35.83% (120) | cholesterol dehydrogenase |
| M. tuberculosis H37Rv | Rv1106c | - | 2e-12 | 35.83% (120) | cholesterol dehydrogenase |
| M. leprae Br4923 | MLBr_01942 | - | 2e-12 | 34.75% (118) | putative cholesterol dehydrogenase |
| M. abscessus ATCC 19977 | MAB_3360c | - | 5e-11 | 27.85% (237) | hypothetical protein MAB_3360c |
| M. marinum M | MMAR_4310 | - | 1e-120 | 68.87% (302) | oxidoreductase |
| M. avium 104 | MAV_1281 | - | 2e-59 | 44.04% (302) | hypothetical protein MAV_1281 |
| M. smegmatis MC2 155 | MSMEG_5799 | - | 1e-127 | 70.92% (306) | nucleoside-diphosphate-sugar epimerase |
| M. thermoresistible (build 8) | TH_0069 | - | 1e-110 | 72.22% (270) | nucleoside-diphosphate-sugar epimerase |
| M. ulcerans Agy99 | MUL_0982 | - | 4e-49 | 46.96% (230) | hypothetical protein MUL_0982 |
| M. vanbaalenii PYR-1 | Mvan_5113 | - | 1e-145 | 81.39% (317) | NAD-dependent epimerase/dehydratase |
CLUSTAL 2.0.9 multiple sequence alignment
Mflv_1635|M.gilvum_PYR-GCK --------------------------------MPGNDVRCVVTGATGYIG
Mvan_5113|M.vanbaalenii_PYR-1 --------------------------------MSTEQVRCLVTGATGYIG
MSMEG_5799|M.smegmatis_MC2_155 --MTSRRWGRFRSECFGQIRGGPTASAPT--RKETVLARCLVTGATGYVG
TH_0069|M.thermoresistible__bu ---------------------------------MPDLPRCLVTGATGYIG
MMAR_4310|M.marinum_M --------------------------------MSAGQVNCLVTGATGYIG
MAV_1281|M.avium_104 -------------------------------------MRVLVTGATGYVG
MUL_0982|M.ulcerans_Agy99 MCSCGQSRCRFRIGSDRLLPDGPASPRPHRLGSSVETMRILVTGATGYVG
Mb1136c|M.bovis_AF2122/97 -----------------------MLRRMGDASLTTELGRVLVTGGAGFVG
Rv1106c|M.tuberculosis_H37Rv -----------------------MLRRMGDASLTTELGRVLVTGGAGFVG
MLBr_01942|M.leprae_Br4923 -----------------------MLRGVGNASLTTELGRVLVTGGSGFVG
MAB_3360c|M.abscessus_ATCC_199 -------------------------------------MKITVVGATGQIG
. *.*.:* :*
Mflv_1635|M.gilvum_PYR-GCK GRLVPELLARGLQVRAMARTPSKLDRTEWRDRVEVVRGDLTEADSLTAAF
Mvan_5113|M.vanbaalenii_PYR-1 RRLVPELLDRGHTVRAMARTPAKLDDAAWRDRVEVVRGDLTEPDSLASAF
MSMEG_5799|M.smegmatis_MC2_155 GRLTPLLVAAGHDVRVLARTPEKLSGVPWRDDVEVARGDLSDLDSLRSAF
TH_0069|M.thermoresistible__bu GRLIPRLLDRGHPVQAMARNPEKLAGAPWRDRVDVVQGDLTDGDSLTAAF
MMAR_4310|M.marinum_M ARLVPRLLDEGHRVRALARDPGKLADVPWRDRAEVVRGDLGDTDSLEAAF
MAV_1281|M.avium_104 SRLVTALLAGGHDVLAATRNPLRLKRFGWFDEITPVPLDAADPASLRSAF
MUL_0982|M.ulcerans_Agy99 SRLVTALLADGHEVLAATRNMARLSRLAWFDDVTPVILDATDRASAQAAM
Mb1136c|M.bovis_AF2122/97 ANLVTTLLDRGHWVRSFDRAPSLLPAHPQLEVLQGDITDADVCAAAVDGI
Rv1106c|M.tuberculosis_H37Rv ANLVTTLLDRGHWVRSFDRAPSLLPAHPQLEVLQGDITDADVCAAAVDGI
MLBr_01942|M.leprae_Br4923 ANLVTALLERGYQVRSFDRAPMPLPQHPQLEVLQGDITDATVCTTAMDSI
MAB_3360c|M.abscessus_ATCC_199 SRVVSLLIADGHDVVAASLSSG---------------ANVLTGEGLVDAL
.: . *: * * : .:
Mflv_1635|M.gilvum_PYR-GCK NG---ADVVYYLVHSMGTSRDFVAE----ERRSAHNVVEAAKKAGVRRIV
Mvan_5113|M.vanbaalenii_PYR-1 DG---VDVIYYLVHSMGTSRDFVAE----EARSARNVVAAAEKAGVRRLV
MSMEG_5799|M.smegmatis_MC2_155 TG---IDVLYYLVHSMGTATDFADS----EREAAANVAEVAKDAGVRRIV
TH_0069|M.thermoresistible__bu AG---VDVVYYLVHSMGSADDFAAE----EARSAREVVAAARRTGVRRLV
MMAR_4310|M.marinum_M AG---MDVVYYLVHSMGSAKHFADE----EARAAHNVVLAARRSGVRRVV
MAV_1281|M.avium_104 AGCGPVEVVYYLVHAIGQP-GFRDA----DKAAAANLAAAAKDAGVRRIV
MUL_0982|M.ulcerans_Agy99 NAAGQIDVVYYLVHGIGQP-DFRDR----DKTAAANLAVAARDTGVRRIV
Mb1136c|M.bovis_AF2122/97 DTIFHTAAIIELMGGASVTDEYRQRSFAVNVGGTENLLHAGQRAGVQRFV
Rv1106c|M.tuberculosis_H37Rv DTIFHTAAIIELMGGASVTDEYRQRSFAVNVGGTENLLHAGQRAGVQRFV
MLBr_01942|M.leprae_Br4923 DTVFHTAAIIELMGGASVTDEYRQRSYTVNVGGTENLLRAGQKSGVKRFV
MAB_3360c|M.abscessus_ATCC_199 TG---SAVVIDVVNSPSFEDGPVMD---FFTASARNLVDAAKATGVGHYV
.: :: . . .: :: ... :** : *
Mflv_1635|M.gilvum_PYR-GCK YLSG---------------LHPD-RKELSRHLASRVEVGEILMQS-GI--
Mvan_5113|M.vanbaalenii_PYR-1 YLGG---------------LHPQGKTDLSRHLASRVEVGDILLGS-TV--
MSMEG_5799|M.smegmatis_MC2_155 YLGG---------------LHPD-DAQLSPHLASRTLVGETLMAS-GV--
TH_0069|M.thermoresistible__bu YLGG---------------LHPP-GTGLSTHLESRVEVGDILLDS-GI--
MMAR_4310|M.marinum_M YLSG---------------LHPE-QSKLSPHLQSRMAVGDALIGS-GI--
MAV_1281|M.avium_104 YLGG---------------FVPA-GEVLSEHLTSRAEVAEALAVPDGP--
MUL_0982|M.ulcerans_Agy99 YLGG---------------FVPE-CEALSQHLSSRAVVAEALAVDDGA--
Mb1136c|M.bovis_AF2122/97 YTSSNSVVMGGQNIAGGDETLPYTDRFNDLYTETKVVAERFVLAQNGV-D
Rv1106c|M.tuberculosis_H37Rv YTSSNSVVMGGQNIAGGDETLPYTDRFNDLYTETKVVAERFVLAQNGV-D
MLBr_01942|M.leprae_Br4923 YTASNSVVMGGTPITGGDETMPYTKRFNDLYTETKVVAEKFVLSQNGVPD
MAB_3360c|M.abscessus_ATCC_199 ALSI----------------VGADGLPESGYMRAKVAQEKIIVGSGLP--
. . : :: :
Mflv_1635|M.gilvum_PYR-GCK ----ETVVLQAGIVIGSGS-ASFEMVRHLTDRLPVMTTPKWVHNKIQPIS
Mvan_5113|M.vanbaalenii_PYR-1 ----ETVVLQAGIVVGSGS-ASFEMVRHLTDRLPVMTTPKWVHNTIQPIA
MSMEG_5799|M.smegmatis_MC2_155 ----ETVVLQAGVVVGSGS-ASFEMIRHLTDRLPVMTTPKWVHNRIQPIA
TH_0069|M.thermoresistible__bu ----ETVVLQAGIVIGSGS-ASFEMIRHLTDRLPVMTTPKWVRNRIQPIA
MMAR_4310|M.marinum_M ----ETIVLQAGVVVGSGS-ASFEMIRHLTDRLPVMTTPKWVHNRIQPIA
MAV_1281|M.avium_104 ----ELVWLGAAMIIGAGS-TSFEMMRYVGDRFPLMPIPSWMDNPIDPIS
MUL_0982|M.ulcerans_Agy99 ----ELVWLGAAMIIGAGS-TLFEMLRYVGDRFPVLPMPDWMYNPIDPIS
Mb1136c|M.bovis_AF2122/97 G--MLTCAIRPSGIWGNGDQTMFRKLFESVLKGHVKVLVGRKSARLDNSY
Rv1106c|M.tuberculosis_H37Rv G--MLTCAIRPSGIWGNGDQTMFRKLFESVLKGHVKVLVGRKSARLDNSY
MLBr_01942|M.leprae_Br4923 GETMLTCSIRPSGIWGRGDQTMFRKAFESVVSGHVKVLIGSKNAKLDNSY
MAB_3360c|M.abscessus_ATCC_199 -----YTIVRAT--------QFQEFAEAITDTLVVGDEVRVPDARIQLIA
: . . : ::
Mflv_1635|M.gilvum_PYR-GCK ISDVLHYLAEAATAEFGE----SRTWDIGGPDVMEYGDAMQGYADVAGLR
Mvan_5113|M.vanbaalenii_PYR-1 IADVLYYLAEAATAEVPE----SRTWDIGGPDVMEYGEAMQIYADIAHLR
MSMEG_5799|M.smegmatis_MC2_155 VRDILHYLVQSASVPFPT----TRAWDVGGPDVLKYGDMMQVYAEEAGLR
TH_0069|M.thermoresistible__bu VDDVLHYLAEAATAAVPG----SRAWDVGGPDVLEYGEAIQVYAEEAGRR
MMAR_4310|M.marinum_M IRDVLHYLVAAATATVPDTAR-ARTWDVGGPDVLEYGDMMRVYAEVAGLH
MAV_1281|M.avium_104 IRDVLHYLLAAADRDLVP----AGAYDIAGPDTTSYRRLLKTYARLSG-R
MUL_0982|M.ulcerans_Agy99 IRDVLHYLVAAADVDQVP----AGAYDICGPDTTSYRELLKTYARIRG-R
Mb1136c|M.bovis_AF2122/97 VHNLIHGFILAAAHLVPDGTAPGQAYFINDAEPINMFEFARPVLEACGQR
Rv1106c|M.tuberculosis_H37Rv VHNLIHGFILAAAHLVPDGTAPGQAYFINDAEPINMFEFARPVLEACGQR
MLBr_01942|M.leprae_Br4923 VHNLVHGLILAAEHLVPGGTAPGQAYFINDGEPINFFDFMGPIIKACGEN
MAB_3360c|M.abscessus_ATCC_199 VDDVSTQVAHAAAAAPRN-----GIINIGGPEKFSFADMAGAVLAARGDD
: :: . :* : . : .
Mflv_1635|M.gilvum_PYR-GCK RRVMVVLPFLTPTIASWWVGLVTPIPSGLARPLVESLECDAICHEHDIDA
Mvan_5113|M.vanbaalenii_PYR-1 RRVMVVLPFLTPTIASLWVGLVTPIPSGLARPLVESLECDAVAHEHDIDT
MSMEG_5799|M.smegmatis_MC2_155 RRRMVVLPWLTPTIASWWVGLVTPIPSGLARPLVESLHCDAICGEHDIDA
TH_0069|M.thermoresistible__bu RRVIVPLPWLTPRIASWWVGLVTPLPPGLAPPX---PRCSATCRS-----
MMAR_4310|M.marinum_M RRYLIVLPFLTPTIASLWVGTVTPIPPGLARPLIESLECDAVMRNSDIDT
MAV_1281|M.avium_104 WHTGLPVGRVDTGLASLVTGVALPVPPGLAGDLVESLDHPMVASGSDLRQ
MUL_0982|M.ulcerans_Agy99 WHAALPVGRLDTAVASRIT----------AG-------HPAGPAWSGRR-
Mb1136c|M.bovis_AF2122/97 WPKMRISGPAVRWVMTGWQRLHFR--FGFPAPLLEPLAVERLYLDNYFSI
Rv1106c|M.tuberculosis_H37Rv WPKMRISGPAVRWVMTGWQRLHFR--FGFPAPLLEPLAVERLYLDNYFSI
MLBr_01942|M.leprae_Br4923 WPRVRISGRLVRNVMAVWQRLHFG--FGLPKPPMEPLAVERVYLDNYFSI
MAB_3360c|M.abscessus_ATCC_199 RPVVVDSGATYFGTPVDDFSLVTG--------------------------
Mflv_1635|M.gilvum_PYR-GCK VIPPPKGGLIGYREAVAKELRSEGGSS-AGRRHLLR---FSPAASD----
Mvan_5113|M.vanbaalenii_PYR-1 VIPPPEGGVTGYRDAVTQALRDDGTAAKGGRRHLLR---FSKVVPQ----
MSMEG_5799|M.smegmatis_MC2_155 VIPPPEGGLTSYRDAVALALACQSRPDTGAAWFNEP---ADSLPSDPDWS
TH_0069|M.thermoresistible__bu --PPSS-------SAPAWSRSGSGCSSAAS--------------------
MMAR_4310|M.marinum_M IIAPPAGGLTNYRRAVELALNRAAHGLPDATWASLQSEPAEPLPSDPRWA
MAV_1281|M.avium_104 RVPDPPGGLLGVDDAIARALDDRSYRRPRPVNALADPHHLADTDP-----
MUL_0982|M.ulcerans_Agy99 -----PGGIAGSPDGGIRGRPAR--QGPRATRRTTEYRRCHYVGA-----
Mb1136c|M.bovis_AF2122/97 AKARRDLGYEPLFTTQQALTECLPYYVSLFEQMKNEAR--AEKTAATVKP
Rv1106c|M.tuberculosis_H37Rv AKARRDLGYEPLFTTQQALTECLPYYVSLFEQMKNEAR--AEKTAATVKP
MLBr_01942|M.leprae_Br4923 EKAHKELGYRPLFTTEQAMAECLPYYTELFEQIKVAAKPHLASIAATPRP
MAB_3360c|M.abscessus_ATCC_199 ------------DDGVLTQTRFADWMARR---------------------
Mflv_1635|M.gilvum_PYR-GCK --------------------------------------------------
Mvan_5113|M.vanbaalenii_PYR-1 --------------------------------------------------
MSMEG_5799|M.smegmatis_MC2_155 --------------GAPAEVAEFRTPSRGADPTATLRNVAEAAWERQGWR
TH_0069|M.thermoresistible__bu --------------------------------------------------
MMAR_4310|M.marinum_M GEIVYTDVRDATTTAAPEAVWKATERAINSRRWYSLALAPRRRHRAPRWR
MAV_1281|M.avium_104 -----------------------GWAGGDARRIRQLAGRVTPAIARPTLG
MUL_0982|M.ulcerans_Agy99 -----------------------GRP-----RERAPVARRRPDRSPPTR-
Mb1136c|M.bovis_AF2122/97 --------------------------------------------------
Rv1106c|M.tuberculosis_H37Rv --------------------------------------------------
MLBr_01942|M.leprae_Br4923 E-------------------------------------------------
MAB_3360c|M.abscessus_ATCC_199 --------------------------------------------------
Mflv_1635|M.gilvum_PYR-GCK --------------------------------------------------
Mvan_5113|M.vanbaalenii_PYR-1 --------------------------------------------------
MSMEG_5799|M.smegmatis_MC2_155 VLPDDPSGPLTLTKDARLGRAWLQFTAEGDGAV----VHRSVLRPRGLLG
TH_0069|M.thermoresistible__bu --------------------------------------------------
MMAR_4310|M.marinum_M VAQRTPTTLRLGAGTRVPGPTWLEVTLTPRGTAGSTYHQRAIFFPRGIPG
MAV_1281|M.avium_104 LVNKVPGPVAGAVRTGLDILIALTPKVRPA--------------------
MUL_0982|M.ulcerans_Agy99 -----------IHRSGVGRR------------------------------
Mb1136c|M.bovis_AF2122/97 --------------------------------------------------
Rv1106c|M.tuberculosis_H37Rv --------------------------------------------------
MLBr_01942|M.leprae_Br4923 --------------------------------------------------
MAB_3360c|M.abscessus_ATCC_199 --------------------------------------------------
Mflv_1635|M.gilvum_PYR-GCK ---------------------------
Mvan_5113|M.vanbaalenii_PYR-1 ---------------------------
MSMEG_5799|M.smegmatis_MC2_155 QVYAATVWPLRRRMLHRRADALFSG--
TH_0069|M.thermoresistible__bu ---------------------------
MMAR_4310|M.marinum_M RLSWLVLRHLYAAALRALARDVTAADR
MAV_1281|M.avium_104 ---------------------------
MUL_0982|M.ulcerans_Agy99 ---------------------------
Mb1136c|M.bovis_AF2122/97 ---------------------------
Rv1106c|M.tuberculosis_H37Rv ---------------------------
MLBr_01942|M.leprae_Br4923 ---------------------------
MAB_3360c|M.abscessus_ATCC_199 ---------------------------