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LRTELGRVLVTGGSGFVGANLVTELLDRGLHVRSFDRVASALPAHARLEIVEGDITDADDVAAAVEGVDT VFHTAAIIDLMGGASVSEEYRQRSFAVNVTGTQNLVHAAQKAGVTRFVYTASNSVVMGGQRIAGGDETLP YTERFNDLYTETKVVAEKFVLSQNGVSGLLTCSIRPSGIWGRGDQTMFRKVFESVLAGHVKVLVGNKETK LDNSYVHNLVHGFILAAEHLVEGGSAPGQAYFINDGEPINMFEFARPVVQACGEPFPKFRVPGRLVWFAM TIWQFLHFKFGLPKPLLEPLAVERLYLDNYFSIAKAQRDLGYQPLFTTEQALAQCIPYYVELFDRMKREA GNPVVQAAAPAPPKG*
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. gilvum PYR-GCK | Mflv_2075 | - | - | 100% (366) | 3-beta hydroxysteroid dehydrogenase/isomerase |
| M. gilvum PYR-GCK | Mflv_1635 | - | 3e-15 | 41.53% (118) | NAD-dependent epimerase/dehydratase |
| M. gilvum PYR-GCK | Mflv_2375 | - | 3e-13 | 25.49% (357) | NAD-dependent epimerase/dehydratase |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb1136c | - | 1e-163 | 78.29% (350) | cholesterol dehydrogenase |
| M. tuberculosis H37Rv | Rv1106c | - | 1e-163 | 78.29% (350) | cholesterol dehydrogenase |
| M. leprae Br4923 | MLBr_01942 | - | 1e-154 | 71.39% (367) | putative cholesterol dehydrogenase |
| M. abscessus ATCC 19977 | MAB_0503 | - | 3e-21 | 29.29% (280) | UDP-glucose 4-epimerase GalE1 |
| M. marinum M | MMAR_4359 | - | 1e-157 | 77.65% (349) | cholesterol dehydrogenase |
| M. avium 104 | MAV_1225 | - | 1e-162 | 78.29% (350) | 3-beta hydroxysteroid dehydrogenase/isomerase family protein |
| M. smegmatis MC2 155 | MSMEG_5228 | - | 1e-163 | 80.75% (348) | 3-beta hydroxysteroid dehydrogenase/isomerase family protein |
| M. thermoresistible (build 8) | TH_1114 | - | 1e-161 | 78.22% (349) | PROBABLE CHOLESTEROL DEHYDROGENASE |
| M. ulcerans Agy99 | MUL_0172 | - | 1e-157 | 77.36% (349) | cholesterol dehydrogenase |
| M. vanbaalenii PYR-1 | Mvan_4635 | - | 0.0 | 87.74% (367) | 3-beta hydroxysteroid dehydrogenase/isomerase |
CLUSTAL 2.0.9 multiple sequence alignment
Mflv_2075|M.gilvum_PYR-GCK --------MLRTELGRVLVTGGSGFVGANLVTELLDRGLHVRSFDRVAS-
Mvan_4635|M.vanbaalenii_PYR-1 ---MGDATTPTTELGRVLVTGGSGFVGANLVTELLDRGLQVRSFDRVPS-
MSMEG_5228|M.smegmatis_MC2_155 ------MADSTTDLGRILVTGGSGFVGANLVTELLDRGYAVRSFDRVPS-
Mb1136c|M.bovis_AF2122/97 MLRRMGDASLTTELGRVLVTGGAGFVGANLVTTLLDRGHWVRSFDRAPS-
Rv1106c|M.tuberculosis_H37Rv MLRRMGDASLTTELGRVLVTGGAGFVGANLVTTLLDRGHWVRSFDRAPS-
MAV_1225|M.avium_104 MIGPMGDPSLTAELGRVLVTGGSGFVGANLVTTLLERGYQVRSFDRAPS-
MMAR_4359|M.marinum_M MLRPMGDASLTTELGRVLVTGGSGFVGSNLVTTLLDRGHHVRSFDRAPS-
MUL_0172|M.ulcerans_Agy99 MLRPMGDASLTTELGRVLVTGGSGFVGSNLVTTLLDRGHHVRSFDRAPS-
MLBr_01942|M.leprae_Br4923 MLRGVGNASLTTELGRVLVTGGSGFVGANLVTALLERGYQVRSFDRAPM-
TH_1114|M.thermoresistible__bu ----MGDPTLRTDLGRVLVTGGSGFVGTNLVTTLLERGHHVRSCDRVGS-
MAB_0503|M.abscessus_ATCC_1997 --------------MRAMVTGAAGFIGSTLVDRLLADGHEVTGLDNLATG
* :***.:**:*:.** ** * * . *.
Mflv_2075|M.gilvum_PYR-GCK ------ALPAHARLEIVEGDITDADDVAAAVEG----VDTVFHTAAIIDL
Mvan_4635|M.vanbaalenii_PYR-1 ------PLPDHPGLEVVQGDITDVDDVARAVGTGADKADTVFHTAAIIDL
MSMEG_5228|M.smegmatis_MC2_155 ------PLPAHAGLEVATGDICDLDNVTNAVAG----VDTVFHTAAIIDL
Mb1136c|M.bovis_AF2122/97 ------LLPAHPQLEVLQGDITDADVCAAAVDG----IDTIFHTAAIIEL
Rv1106c|M.tuberculosis_H37Rv ------LLPAHPQLEVLQGDITDADVCAAAVDG----IDTIFHTAAIIEL
MAV_1225|M.avium_104 ------PLPAHPHLEVLQGDITDAGVCAAAVEG----IDTIFHTAAIIDL
MMAR_4359|M.marinum_M ------PLPPHPQLEVLQGDITDAATCATAVDG----VDTVFHTAAIIEL
MUL_0172|M.ulcerans_Agy99 ------PLPPHPQLEVLQGDITDAATCATAVDG----VDTVFHTAAIIEL
MLBr_01942|M.leprae_Br4923 ------PLPQHPQLEVLQGDITDATVCTTAMDS----IDTVFHTAAIIEL
TH_1114|M.thermoresistible__bu ------PLPDHPRLEVIDGDICDADAVAAAVDG----IDTVFHTAAVIDL
MAB_0503|M.abscessus_ATCC_1997 KVANLEAAEANAKFTFVKDDIVEADLDAVVAEYS---PEVIFHLAAQIDV
:. : . .** : : . :.:** ** *::
Mflv_2075|M.gilvum_PYR-GCK MGGASVSEEYRQRSFAVNVTGTQNLVHAAQKAGVTRFVYTASNSVVMGGQ
Mvan_4635|M.vanbaalenii_PYR-1 MGGASVTEEYRQRSFAVNVTGTKNLVHAAQKAGVQRFVYTASNSVVMGGQ
MSMEG_5228|M.smegmatis_MC2_155 MGGASVTAEYRQRSFAVNVGGTENLVRAAQSAGVKRFVYTASNSVVMGGQ
Mb1136c|M.bovis_AF2122/97 MGGASVTDEYRQRSFAVNVGGTENLLHAGQRAGVQRFVYTSSNSVVMGGQ
Rv1106c|M.tuberculosis_H37Rv MGGASVTDEYRQRSFAVNVGGTENLLHAGQRAGVQRFVYTSSNSVVMGGQ
MAV_1225|M.avium_104 MGGASVTEEYRQRSFAVNVGGTENLVRAGQAAGVQRFVYTSSNSVVMGGQ
MMAR_4359|M.marinum_M MGGASVTDEYRKRSFSVNVGGTENLVREGQQAGVKRFVYTSSNSVVMGGQ
MUL_0172|M.ulcerans_Agy99 MGGASVTDEYRKRSFSVNVGGTENLVRAGQQAGVKRFVYTSSNSVVMGGQ
MLBr_01942|M.leprae_Br4923 MGGASVTDEYRQRSYTVNVGGTENLLRAGQKSGVKRFVYTASNSVVMGGT
TH_1114|M.thermoresistible__bu MGGAGVTDEYRERSFAVNVHGTERLVRAAQQAGVRRFVYTASNSVVMGGQ
MAB_0503|M.abscessus_ATCC_1997 R--HSVADPQFDS--SVNVVGTVRLAEAARKAGVRKIVHTSSGGSIYGTP
.*: . :*** ** .* . .: :** ::*:*:*.. : *
Mflv_2075|M.gilvum_PYR-GCK RIAGGDETLPYTERFNDLYTETKVVAEKFVLSQNGV---SGLLTCSIRPS
Mvan_4635|M.vanbaalenii_PYR-1 RIAGGDETLPYTERFNDLYTETKVVAEKFVLSQNGV---SGMLTCSIRPS
MSMEG_5228|M.smegmatis_MC2_155 HIVHGDETLPYTERFNDLYTETKVVAEKFVLGRNGV---AGMLTCSIRPS
Mb1136c|M.bovis_AF2122/97 NIAGGDETLPYTDRFNDLYTETKVVAERFVLAQNGV---DGMLTCAIRPS
Rv1106c|M.tuberculosis_H37Rv NIAGGDETLPYTDRFNDLYTETKVVAERFVLAQNGV---DGMLTCAIRPS
MAV_1225|M.avium_104 NIVGGDETLPYTDRFNDLYTETKVLAERFVLGQNGV---DGMLTCAIRPS
MMAR_4359|M.marinum_M IIAGGDETLPYTNRFNDLYTETKVVAEKFVLSQNGN---NEMLTCAIRPS
MUL_0172|M.ulcerans_Agy99 IIAGGDETLPYTNRFNDLYTETKVVAEKFVLSQNGN---NEMLTCAIRPS
MLBr_01942|M.leprae_Br4923 PITGGDETMPYTKRFNDLYTETKVVAEKFVLSQNGVPDGETMLTCSIRPS
TH_1114|M.thermoresistible__bu PIADGDETLPYTDRFNDLYTETKVIAERFVLGQNGI---EGMLTCSIRPS
MAB_0503|M.abscessus_ATCC_1997 KQIPTSETVPTDP--HSPYAAGKVAGEIYLNTFRHL---YGLECSHIAPA
.**:* :. *: ** .* :: . : . * *:
Mflv_2075|M.gilvum_PYR-GCK GIWG-----RGDQTMFRKVFESVLAGHVKVLVGNKETKLDNSYVHNLVHG
Mvan_4635|M.vanbaalenii_PYR-1 GIWG-----RGDQTMFRKVFESVLAGHVKVLVGNKNVKLDNSYVHNLVHG
MSMEG_5228|M.smegmatis_MC2_155 GIWG-----RGDQTMFRKVFESVLAGHVKVLVGRKSTLLDNSYVHNLVHG
Mb1136c|M.bovis_AF2122/97 GIWG-----NGDQTMFRKLFESVLKGHVKVLVGRKSARLDNSYVHNLIHG
Rv1106c|M.tuberculosis_H37Rv GIWG-----NGDQTMFRKLFESVLKGHVKVLVGRKSARLDNSYVHNLIHG
MAV_1225|M.avium_104 GIWG-----RGDQTMFRKLFESVIAGHVKVLIGRKSARLDNSYVHNLIHG
MMAR_4359|M.marinum_M GIWG-----TGDQLMFRKLFESVIKGHVKVLVGPKSALLDNSYVDNLIHG
MUL_0172|M.ulcerans_Agy99 GIWG-----TGDQLMFRKLFESVIKGHVKVLVGPKSVLLDNSYVDNLIHG
MLBr_01942|M.leprae_Br4923 GIWG-----RGDQTMFRKAFESVVSGHVKVLIGSKNAKLDNSYVHNLVHG
TH_1114|M.thermoresistible__bu GIWG-----PGDQTMFRKLFESVHAGHVKVLIGSRHARLDNSYVHNLVHG
MAB_0503|M.abscessus_ATCC_1997 NVYGPRQDPHGEAGVVAIFAQALLSGRSTKVFGDGGNTRDYVFVDDVVEA
.::* *: :. ::: *: . :.* * :*.:::..
Mflv_2075|M.gilvum_PYR-GCK FILAAEHLVEGGSAPGQAYFINDGEPINMFEFARPVVQACGEPFPKFRVP
Mvan_4635|M.vanbaalenii_PYR-1 FILAAQHLVDGGTAPGQAYFINDGEPINMFEFARPVMEACGEPWPTFRVS
MSMEG_5228|M.smegmatis_MC2_155 FILAAEHLTPNGTAPGQAYFINDGEPVNMFEFARPVIEACGRKLPRVRVP
Mb1136c|M.bovis_AF2122/97 FILAAAHLVPDGTAPGQAYFINDAEPINMFEFARPVLEACGQRWPKMRIS
Rv1106c|M.tuberculosis_H37Rv FILAAAHLVPDGTAPGQAYFINDAEPINMFEFARPVLEACGQRWPKMRIS
MAV_1225|M.avium_104 FILAAQHLTPGGTAPGQAYFINDAEPINMFEFARPVVEACGVNWPRVRVN
MMAR_4359|M.marinum_M FILAAQHLVPGGSAPGQAYFINDAEPINMFEFARPVVEACGEKWPRVRVS
MUL_0172|M.ulcerans_Agy99 FMLAAQHLVPGGSAPGQAYFINDAEPINMFDFARPVVEACGEKWPRVRVS
MLBr_01942|M.leprae_Br4923 LILAAEHLVPGGTAPGQAYFINDGEPINFFDFMGPIIKACGENWPRVRIS
TH_1114|M.thermoresistible__bu FILAAEHLVPGGTAPGQAYFINDGEPINMFEFARPVVTACGQRWPTLRVS
MAB_0503|M.abscessus_ATCC_1997 FVRAS-----GPDGGGQRFNVGTGVETSDRQLHSLVAAAAGAPD------
:: *: . . ** : :. . . :: : *.*
Mflv_2075|M.gilvum_PYR-GCK GRLVWFAMTIWQFLHFKFGLPKPLLEPLAVERLYLDNYFSIAKAQRDLGY
Mvan_4635|M.vanbaalenii_PYR-1 GRLVWFAMTIWQFLHFRFGLPKPLLEPLAVERLYLDNYFSIAKAERDLGY
MSMEG_5228|M.smegmatis_MC2_155 GRAVHAAMSGWQRLHFRFGIPEPLLEPLAVERLYLNNYFSIAKATRDLGY
Mb1136c|M.bovis_AF2122/97 GPAVRWVMTGWQRLHFRFGFPAPLLEPLAVERLYLDNYFSIAKARRDLGY
Rv1106c|M.tuberculosis_H37Rv GPAVRWVMTGWQRLHFRFGFPAPLLEPLAVERLYLDNYFSIAKARRDLGY
MAV_1225|M.avium_104 GPIVRAAMTGWQRLHFRFGIPAPLLEPLAVERLYLDNFFSIAKASRDLGY
MMAR_4359|M.marinum_M GPMVHRAMTGWQRLHFRFGLPAPLLEPLAVERLYLDNYFSVDKARRDLGY
MUL_0172|M.ulcerans_Agy99 GPMVHRAMTGWQRLHFRFGLPAPLLEPLAVERLYLDNYFSVDKARRDLGY
MLBr_01942|M.leprae_Br4923 GRLVRNVMAVWQRLHFGFGLPKPPMEPLAVERVYLDNYFSIEKAHKELGY
TH_1114|M.thermoresistible__bu GPLVRAVMSLWQRLHFRFGLPKPPLEPLAVERLYLDNYFSIDKARRELGY
MAB_0503|M.abscessus_ATCC_1997 ---------------------DPEFHPPRLGDLRQS-CLDVGLAKRVLGW
* :.* : : . :.: * : **:
Mflv_2075|M.gilvum_PYR-GCK QPLFTTEQALAQCIPYYVELFDRMKREAGNPVVQAAAPAPPKG
Mvan_4635|M.vanbaalenii_PYR-1 RPLYTTEQALEHCIPYYVDLFGRMKAEAAQPAVQAAAPVPPAG
MSMEG_5228|M.smegmatis_MC2_155 RPLFTTEQARVDCLPYYVDLFKQMEAQAR-PA-----------
Mb1136c|M.bovis_AF2122/97 EPLFTTQQALTECLPYYVSLFEQMKNEARAEKTAATVKP----
Rv1106c|M.tuberculosis_H37Rv EPLFTTQQALTECLPYYVSLFEQMKNEARAEKTAATVKP----
MAV_1225|M.avium_104 QPLFTTEQAMSECLPYYVGMFEQMKRQALAGKASA--------
MMAR_4359|M.marinum_M EPKFTTEQALKECLPYYVDLFRQMKSEVKAGGR----------
MUL_0172|M.ulcerans_Agy99 EPKFTTEQALKECLPYYVDLFRQMKSEVKAGGR----------
MLBr_01942|M.leprae_Br4923 RPLFTTEQAMAECLPYYTELFEQIKVAAKPHLASIAATPRPE-
TH_1114|M.thermoresistible__bu RPRYTTEQALQECLPYYVDLFHRMKAEPATV------------
MAB_0503|M.abscessus_ATCC_1997 EPRVPLADGIRRTVDFFRS------------------------
.* . :. : ::