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SAHVLGAQIDRKVRPVDSMAPDATTMRTLENARGSILKGRLPASLIANAALYELELKRVFGRTWQFLCHE DEIPNAGDYVVRYIADNSIIVARQQDMTIRAMSNSCRHRGTLLCRTESGNESAFQCPYHGWTYRNNGDLI AIPAQQAVYGAAFDKSRLGLRALPMLDSYAGLVFGCVSDEAPGLDEYLGDMRWYLDLMMKKSPAGLEAWG APQRWVIDANWKTGADNFVGDGYHTVMTHRSMCELGLLPPDNVAVSPAHVSLSGGHGAGVLGAPPGIPAP PYMGYPEEVVSGLSEGYGDDVHGELLKRTMFIHGNVFPNLSFLNAFIAKDGESMPVPILTLRQWRPLDAA RMEVWSWFFVERNAPEEFKQQSFETYVRTFGVGGVFEQDDAEIFQAITKGTRGELAGGVELNLEMGLDNL APDPTWLGPGRPLASGYAEQNQREYWKQYFDYLATPRRDENV*
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. gilvum PYR-GCK | Mflv_0640 | - | - | 100% (463) | ring hydroxylating dioxygenase, alpha subunit |
| M. gilvum PYR-GCK | Mflv_0558 | - | 0.0 | 100.00% (463) | ring hydroxylating dioxygenase, alpha subunit |
| M. gilvum PYR-GCK | Mflv_0537 | - | e-165 | 63.27% (422) | ring hydroxylating dioxygenase, alpha subunit |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb3186c | - | 7e-18 | 29.91% (214) | dioxygenase |
| M. tuberculosis H37Rv | Rv3161c | - | 5e-18 | 29.91% (214) | dioxygenase |
| M. leprae Br4923 | - | - | - | - | - |
| M. abscessus ATCC 19977 | MAB_4370c | - | 2e-65 | 35.20% (429) | putative large terminal subunit of phenylpropionate dioxygenase |
| M. marinum M | MMAR_0212 | - | 2e-20 | 27.95% (254) | oxidoreductase |
| M. avium 104 | MAV_3509 | - | 7e-81 | 39.22% (459) | phthalate dioxygenase large subunit |
| M. smegmatis MC2 155 | MSMEG_1908 | - | 4e-39 | 26.42% (424) | benzoate 1,2-dioxygenase alpha subunit |
| M. thermoresistible (build 8) | TH_2976 | - | 4e-37 | 29.79% (423) | PUTATIVE dioxygenase large subunit |
| M. ulcerans Agy99 | MUL_3820 | - | 2e-16 | 30.73% (179) | ring-hydroxylating dioxygenase, large terminal subunit |
| M. vanbaalenii PYR-1 | Mvan_0525 | - | 0.0 | 99.78% (463) | ring hydroxylating dioxygenase, alpha subunit |
CLUSTAL 2.0.9 multiple sequence alignment
Mb3186c|M.bovis_AF2122/97 ------------MLSTDNRAELGDILTDIGDYLDDNPPALSLPP--AAYT
Rv3161c|M.tuberculosis_H37Rv ------------MLSTDNRAELGDILTDIGDYLDDNPPALSLPP--AAYT
Mflv_0640|M.gilvum_PYR-GCK MSAHVLGAQIDRKVRPVDSMAPDATTMRTLENARGSILKGRLPA--SLIA
Mvan_0525|M.vanbaalenii_PYR-1 MSAHVLGAQIDRKVRPVDSMAPDATTMRTLENARGSILKGRLPA--SLIA
MAV_3509|M.avium_104 ------------------MAGPDGGLLGVLENVR----RGMIPA--HIYN
MAB_4370c|M.abscessus_ATCC_199 ----MASLPRPVAVAQGALCRPQALVRDIDVSGLVDSHGGLLDR--VIFT
MSMEG_1908|M.smegmatis_MC2_155 ---------------MTTHLSPDHLENVLADAVIEDPAAGVYRANRRIFT
TH_2976|M.thermoresistible__bu ------------------LIRPDRVHG-------------------SLYT
MMAR_0212|M.marinum_M -----------------MSTAAGNPARTRDEDAIGVPPASPTLVPAQRYH
MUL_3820|M.ulcerans_Agy99 -------------MDRDQLIDLTRRALKLARDKTTDLAPHQHAVDAREYT
Mb3186c|M.bovis_AF2122/97 SSELWQLERERIFNRSWMLVAHVDQLAKTGDYVTVSVAGEPVMVVRDVDG
Rv3161c|M.tuberculosis_H37Rv SSELWQLERERIFNRSWMLVAHVDQVAKTGDYVTVSVAGEPVMVVRDVDG
Mflv_0640|M.gilvum_PYR-GCK NAALYELELKRVFGRTWQFLCHEDEIPNAGDYVVRYIADNSIIVARQQDM
Mvan_0525|M.vanbaalenii_PYR-1 NAALYALELKRVFGRTWQFLCHEDEIPNAGDYVVRYIADNSIIVARQQDM
MAV_3509|M.avium_104 DPELFALEKRRLFARAWTFVGHESEIPHDGDYMVRRVLDDSFIITRGSDG
MAB_4370c|M.abscessus_ATCC_199 DGELYRQELRRVFAPSWLFLAHADQFHRPGDFFTTYMGEDPVIVSMDKNR
MSMEG_1908|M.smegmatis_MC2_155 DDEIFELEMKHIFEGNWVYLAHESQVPNPGDYFTTYIGRQPVVITRDRDG
TH_2976|M.thermoresistible__bu DPAIFAEELRKIWYRTWVFVGHESEVSQPNDYVRKKLGLQDVIMTRDRDG
MMAR_0212|M.marinum_M SPAFAQLEIERMWPKVWQLACMLDHVAAPGDYFEYRCGPYSVLIVHDCDG
MUL_3820|M.ulcerans_Agy99 SLDRHERDRAMVLD-SPQLVGYSSELPGPDTYRTKTIMGRSILLTRAADR
. : : ... . : .:: :
Mb3186c|M.bovis_AF2122/97 QLHALSPICRHRLMLMVEPGAGRIDTLTCQYHLWRYGLDGRLRGAPHMAA
Rv3161c|M.tuberculosis_H37Rv QLHALSPICRHRLMLMVEPGAGRIDTLTCQYHLWRYGLDGRLRGAPHMAA
Mflv_0640|M.gilvum_PYR-GCK TIRAMSNSCRHRGTLLCRTESGNESAFQCPYHGWTYRNNGDLIAIPAQQA
Mvan_0525|M.vanbaalenii_PYR-1 TIRAMSNSCRHRGTLLCRTESGNESAFQCPYHGWTYRNNGDLIAIPAQQA
MAV_3509|M.avium_104 RVRALFNMCLHRGMQVCRAELGNASNFRCPYHGWTYRNDGRLTGLPFHRE
MAB_4370c|M.abscessus_ATCC_199 RIRAFLNSCRHRGARVCRADYGVTRGFTCTYHGWAYDLEGKLISVPNEEG
MSMEG_1908|M.smegmatis_MC2_155 DLHCLINACAHRGAMICRRKTDNRTTLTCPFHGWTFRNDGTLLKVKDPDG
TH_2976|M.thermoresistible__bu EIHLLLNRCSHRGNQVCADAKGNSSTFRCPYHGWTFRNTGELVGFPFFKG
MMAR_0212|M.marinum_M VLRAFQNVCRHRGNSLCAGSGSGLRELKCGYHGWTWDLTGELRRVPNRKG
MUL_3820|M.ulcerans_Agy99 AVRAFDNVCLHRQSRVATG-CGTALRFTCPYHGWTYDNGGRLVGLPGREG
:: : * ** : . : * :* * : * *
Mb3186c|M.bovis_AF2122/97 NLD-----FNRRECRLPQFAVATWNGLVWINLDADA-EPIAAHLDLTDDE
Rv3161c|M.tuberculosis_H37Rv NLD-----FNRRECRLPQFAVATWNGLVWINLDADA-EPIAAHLDLTDDE
Mflv_0640|M.gilvum_PYR-GCK VYG--AAFDKSRLGLRALPMLDSYAGLVFGCVSDEA-PGLDEYLGDMRWY
Mvan_0525|M.vanbaalenii_PYR-1 VYG--AAFDKSRLGLRALPMLDSYAGLVFGCVSDEA-PGLDEYLGDMRWY
MAV_3509|M.avium_104 AYGGDDGFVKDQT-LLPAPNFAGYNGLLFISLDPAA-PPLQDFLGDFRFY
MAB_4370c|M.abscessus_ATCC_199 YPD---SFSTDDWGLVEVPHVDSYHGLIFGTWNPAP-VGLRESLGDMAWY
MSMEG_1908|M.smegmatis_MC2_155 AGYPDTFNSNGSHNMTNVARFEGYRGFLFGSLNPDV-LPLTEHLGDTTTV
TH_2976|M.thermoresistible__bu YGT-----RKLDLPLGRVPRVDSYGGFVFGSFATEG-PTLREHLGAAAGE
MMAR_0212|M.marinum_M FGS-----LHMPDFPLLPVRVETWEGLVFVSLDVTA-MPLMDYLDPIPED
MUL_3820|M.ulcerans_Agy99 FPG-----IAVGSDGLTELPATEFAGFLWVSLHPAARLDVPAHLGPLAGE
: *::: : *.
Mb3186c|M.bovis_AF2122/97 FAGYRLGEMVQ-VESWS----HEWRANWKVAAENGHEN-YHVLGLHRQTL
Rv3161c|M.tuberculosis_H37Rv FAGYRLGEMVQ-VESWS----HEWRANWKVAAENGHEN-YHVLGLHRQTL
Mflv_0640|M.gilvum_PYR-GCK LDLMMKKSPAG-LEAWGAPQRWVIDANWKTGADNFVGDGYHTVMTHRSMC
Mvan_0525|M.vanbaalenii_PYR-1 LDLMMKKSPAG-LEAWGAPQRWVIDANWKTGADNFVGDGYHTVMTHRSMC
MAV_3509|M.avium_104 LDYYTRQSAGG-VELRG-PQRWRIRANWKIGAENFAGDMYHTPHTHASIV
MAB_4370c|M.abscessus_ATCC_199 MDAMLDHDSEG-TVVVGGVHKWVLDGNWKLAAEQFATDWYHVNMSHASAL
MSMEG_1908|M.smegmatis_MC2_155 IDMLVDQSPDG-LEVLRGASTYTYDGNWKVQAEN-GADGYHVTATHWNYA
TH_2976|M.thermoresistible__bu IDRLLRLSPEGKVALDAGWLRHRTRANWKLLVEN-ETDGYHPQFVHGGIF
MMAR_0212|M.marinum_M IAWCGLSDFRC-----YATLTVEVDANWKTIADG-YSETYHIQTLHPELL
MUL_3820|M.ulcerans_Agy99 LQSWGIGRWSP-----LGEKVLGSPIDWKLATDTFAEN-YHFATVHRRTF
: :** .: : ** *
Mb3186c|M.bovis_AF2122/97 EP---------------------------FVPGGGDLDVRQYSRWALRLR
Rv3161c|M.tuberculosis_H37Rv EP---------------------------FVPGGGDLDVRQYSRWALRLR
Mflv_0640|M.gilvum_PYR-GCK ELGLLPPDNVAVSPAHVSLSGGHGAG-VLGAPPG--IPAPPYMGYPEEVV
Mvan_0525|M.vanbaalenii_PYR-1 ELGLLPPDNVAVSPAHVSLSGGHGAG-VLGAPPG--IPAPPYMGYPEEVV
MAV_3509|M.avium_104 DIGLFREPKAQKRKDGATYWAHRGGGTTYKLPPGGFEERMRYVGYPDEMI
MAB_4370c|M.abscessus_ATCC_199 MVMAPQGKAPKDEIVHRTGRQYVDPN-----GHGAGFPVHPKNRFDARVV
MSMEG_1908|M.smegmatis_MC2_155 ATTSRRTCGESKNDTKALDAGSWGKSGGGYWSFPHGHLCLWTWAANPQDR
TH_2976|M.thermoresistible__bu GVTGSTIGPLYSDSSTAVTRDLGGGHSENDLRPEFRKFAEPLRWFGTTES
MMAR_0212|M.marinum_M RCVDDIHAPQQIWRHAGKSDQPYGVPSPRFEGTLSDQEVWDAYVYTQGAL
MUL_3820|M.ulcerans_Agy99 ATIAR-------------------------------SNCTVFDSYGPHHR
Mb3186c|M.bovis_AF2122/97 VPFTVP--VEAKSLQLNEVQKSN-LVVLWTFPNSALAIAG----------
Rv3161c|M.tuberculosis_H37Rv VPFTVP--VEAKSLQLNEVQKSN-LVVLWTFPNSALAIAG----------
Mflv_0640|M.gilvum_PYR-GCK SGLSEG--YGDDVHGELLKRTMF-IHGN-VFPNLSFLNAF-IAKDG-ESM
Mvan_0525|M.vanbaalenii_PYR-1 SGLSEG--YGDDVHGELLKRTMF-IHGN-VFPNLSFLNAF-IAKDG-ESM
MAV_3509|M.avium_104 GRIKQV--WTPRQQRVVGEDGFM-VSAATCFPNLSFVHNWPKVRDGRDDE
MAB_4370c|M.abscessus_ATCC_199 HQWTDYGALRERLGEARVQGPLT-NGHATVFPNLSYLPVN----------
MSMEG_1908|M.smegmatis_MC2_155 PLWDRLDELKARFGDAKGEFMVKGSRNLCLYPNVYVMDQF----------
TH_2976|M.thermoresistible__bu RVPDYVAAMRARYGAEADQILIEGSPHVMIFPNLFIAEIQ----------
MMAR_0212|M.marinum_M MGAAEGTPLPADERRPGQSVQDLIAARTRAFAATRGVDLEWADTDRITRL
MUL_3820|M.ulcerans_Agy99 LIFPLNTILELENVPEGQWDPFHHMVVIYALFPNVVLSVT----------
Mb3186c|M.bovis_AF2122/97 --ERVVWFGFIPQSIDRVQVLGGVLTTP----ELAADAAATAQTSQFVMA
Rv3161c|M.tuberculosis_H37Rv --ERVVWFGFIPQSIDRVQVLGGVLTTP----ELAADAAATAQTSQFVMA
Mflv_0640|M.gilvum_PYR-GCK PVPILTLRQWRPLDAARMEVWSWFFVERNAPEEFKQQSFETYVRTFGVGG
Mvan_0525|M.vanbaalenii_PYR-1 PVPILTLRQWRPLDAARMEVWSWFFVERNAPEEFKQQSFETYVRTFGVGG
MAV_3509|M.avium_104 TLPFISIRLWQPISENETEVCSWFAVDSAAPAHYKQDSYKAYLMCFGSTG
MAB_4370c|M.abscessus_ATCC_199 ----GSVRIWHPKGPDKMEVWAWTIVDRSMPEEVRDAQRLYNLRTFGPTG
MSMEG_1908|M.smegmatis_MC2_155 ---STQIRHFRPIAPDKTEVTIYCIAPKGESAAARAHRIRQYEDFFNASG
TH_2976|M.thermoresistible__bu ------VFNIQPVAVNECVQYSTAVQLVGAP-ELNRRMVSQCIGSVGPAG
MMAR_0212|M.marinum_M HQYNVFPNMTFLANADHLTIMCSRPQPEHFADPDKGEMVMFLMTRMPPDA
MUL_3820|M.ulcerans_Agy99 ----IANGELFRIYPGDRPGSSITVHQNSTPLDLSDESVATGAQAVFDYA
.
Mb3186c|M.bovis_AF2122/97 MINDEDRLGLEAVQVGAGSRFAERGHLSSKE-------------------
Rv3161c|M.tuberculosis_H37Rv MINDEDRLGLEAVQVGAGSRFAERGHLSSKE-------------------
Mflv_0640|M.gilvum_PYR-GCK VFEQDDAEIFQAITKGTRGELAGGVELNLEMGLDN--------LAPDPTW
Mvan_0525|M.vanbaalenii_PYR-1 VFEQDDAEIFQAITKGTRGELAGGVELNLEMGLDN--------LAPDPTW
MAV_3509|M.avium_104 MFEQDDVENWVSLTTTAGGSMARRLLLNSRMGLYDDGRPVVEALAPS-AF
MAB_4370c|M.abscessus_ATCC_199 IFEQDDGENWSEVQSISRGFVTNSVALNYQMGLDS-----------ERED
MSMEG_1908|M.smegmatis_MC2_155 MATPDDLEEFRSCQLTFRAQAAPWNDMSRGAQHWLSG------PDPVAES
TH_2976|M.thermoresistible__bu MLLADDTEMYERNQQGLEALTPEWLDIRRGLNREH--------------V
MMAR_0212|M.marinum_M PHNKPTDVRMAADQAEPGIVLTQDIRVLPGVQRGLHQPG-----FTHLVL
MUL_3820|M.ulcerans_Agy99 HATVRDEDYRLAETLQTNLESGARQRLVFGR-------------------
:
Mb3186c|M.bovis_AF2122/97 -------WPGMLAFYRNLAMALVGDHPGAS-----------
Rv3161c|M.tuberculosis_H37Rv -------WPGMLAFYRNLAMALVGDHPGAS-----------
Mflv_0640|M.gilvum_PYR-GCK LGPGRPLASGYAEQNQREYWKQYFDYLATPRRDENV-----
Mvan_0525|M.vanbaalenii_PYR-1 LGPGRPLASGYAEQNQREYWKQYFDYLATPRRDENV-----
MAV_3509|M.avium_104 HGPGR-AQVGYNEHNQRALLAMWADYLQEGDACENR-----
MAB_4370c|M.abscessus_ATCC_199 GRHPGLTSELYSDAAGRSFYARWRDLMNTKAWHEEHV----
MSMEG_1908|M.smegmatis_MC2_155 LGMHGIVSSGLRNEDEGLYPVQHGYWLETLRRAVTSNPTEH
TH_2976|M.thermoresistible__bu DDHGFTVGGPTDETGMRAFWSEYRRLMEAE-----------
MMAR_0212|M.marinum_M SGEERRIINMHRNLERYLDLPEPERMSGGGLRPGPGAPRV-
MUL_3820|M.ulcerans_Agy99 --------NEPGLQHRHITWAQALETPG-------------