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ETIGSKTGPALPIDSSAFYDEAVYQRELDSIFKRSWLFVGHESMIPKPGDFRTTYMADDAVIVCRDKESR VRVLLNKCRHRGNKVCQFDKGNANIFHCSYHGWSYDTAGRLRSVPLAESAYGPQFDKASMGLVSPRVATY KGLIFACWDQSAPPLEEYLGEGLLWYLDNFLLDCDPNGLQVVPGLHRYLMPVNWKLLAENFGGDQYHFAA THGSVSALSKAGQTARINFSIDEGQHYSVVLDGCAPHGLLQLAVGKNFYQDDLAQAETLGTEAVDWLTER QRLQDERLATSPVQPYSFHVANIFPNFSMIGMGTAFYGRGFIMWQPRGPRLTEVWEWCLVESSAPRAVKE RMVFVLSQRQSAAGLVTPDDHENFERLSDALDTGVARDVPFNYSLGEDVEPMESLVAELPGNVRPQISEA YQREFYRHWHRTMTEPA*
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. gilvum PYR-GCK | Mflv_0626 | - | - | 100% (438) | Rieske (2Fe-2S) domain-containing protein |
| M. gilvum PYR-GCK | Mflv_0544 | - | 0.0 | 99.77% (438) | Rieske (2Fe-2S) domain-containing protein |
| M. gilvum PYR-GCK | Mflv_0632 | - | 1e-65 | 35.19% (432) | ring hydroxylating dioxygenase, alpha subunit |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb3186c | - | 2e-21 | 31.43% (210) | dioxygenase |
| M. tuberculosis H37Rv | Rv3161c | - | 1e-21 | 31.43% (210) | dioxygenase |
| M. leprae Br4923 | - | - | - | - | - |
| M. abscessus ATCC 19977 | MAB_4370c | - | 3e-61 | 34.66% (427) | putative large terminal subunit of phenylpropionate dioxygenase |
| M. marinum M | MMAR_4744 | - | 5e-22 | 29.34% (259) | dioxygenase |
| M. avium 104 | MAV_3509 | - | 2e-47 | 31.19% (436) | phthalate dioxygenase large subunit |
| M. smegmatis MC2 155 | MSMEG_1908 | - | 1e-33 | 27.49% (371) | benzoate 1,2-dioxygenase alpha subunit |
| M. thermoresistible (build 8) | TH_2976 | - | 2e-32 | 37.25% (204) | PUTATIVE dioxygenase large subunit |
| M. ulcerans Agy99 | MUL_3820 | - | 6e-14 | 26.34% (243) | ring-hydroxylating dioxygenase, large terminal subunit |
| M. vanbaalenii PYR-1 | Mvan_0539 | - | 0.0 | 99.77% (438) | Rieske (2Fe-2S) domain-containing protein |
CLUSTAL 2.0.9 multiple sequence alignment
Mflv_0626|M.gilvum_PYR-GCK ---------------------METIGSKTG---PALPIDSSAFYDEAVYQ
Mvan_0539|M.vanbaalenii_PYR-1 ---------------------METIGSKTG---SALPIDSSAFYDEAVYQ
Mb3186c|M.bovis_AF2122/97 --------MLSTDNRAELGDILTDIGDYLDDNPPALSLPPAAYTSSELWQ
Rv3161c|M.tuberculosis_H37Rv --------MLSTDNRAELGDILTDIGDYLDDNPPALSLPPAAYTSSELWQ
MAB_4370c|M.abscessus_ATCC_199 MASLPRPVAVAQGALCRPQALVRDIDVSGLVDSHGGLLDRVIFTDGELYR
MAV_3509|M.avium_104 --------------MAGPDGGLLGVLENVR----RGMIPAHIYNDPELFA
MMAR_4744|M.marinum_M --------------MPHFTKPAAGSWTQNYPELGTAPVDYTDSIDPQYFA
TH_2976|M.thermoresistible__bu -------------------------------LIRPDRVHGSLYTDPAIFA
MSMEG_1908|M.smegmatis_MC2_155 ---------MTTHLSPDHLENVLADAVIEDPAAGVYRANRRIFTDDEIFE
MUL_3820|M.ulcerans_Agy99 -------MDRDQLIDLTRRALKLARDKTTDLAPHQHAVDAREYTSLDRHE
.
Mflv_0626|M.gilvum_PYR-GCK RELDSIFKRSWLFVGHESMIPKPGDFRTTYMADD----AVIVCRDKESRV
Mvan_0539|M.vanbaalenii_PYR-1 RELDSIFKRSWLFVGHESMIPKPGDFRTTYMADD----AVIVCRDKESRV
Mb3186c|M.bovis_AF2122/97 LERERIFNRSWMLVAHVDQLAKTGDYVTVSVAGE----PVMVVRDVDGQL
Rv3161c|M.tuberculosis_H37Rv LERERIFNRSWMLVAHVDQVAKTGDYVTVSVAGE----PVMVVRDVDGQL
MAB_4370c|M.abscessus_ATCC_199 QELRRVFAPSWLFLAHADQFHRPGDFFTTYMGED----PVIVSMDKNRRI
MAV_3509|M.avium_104 LEKRRLFARAWTFVGHESEIPHDGDYMVRRVLDD----SFIITRGSDGRV
MMAR_4744|M.marinum_M DEQRAIFRRTWLNVGRIERLPRTGSYFTKELPSTGAGMSVIVVKGKDGVI
TH_2976|M.thermoresistible__bu EELRKIWYRTWVFVGHESEVSQPNDYVRKKLGLQ----DVIMTRDRDGEI
MSMEG_1908|M.smegmatis_MC2_155 LEMKHIFEGNWVYLAHESQVPNPGDYFTTYIGRQ----PVVITRDRDGDL
MUL_3820|M.ulcerans_Agy99 RDRAMVLD-SPQLVGYSSELPGPDTYRTKTIMGR----SILLTRAADRAV
: : :. . . . : : .:: : :
Mflv_0626|M.gilvum_PYR-GCK RVLLNKCRHRGNKVCQFDK------GNANIFHCSYHGWSYDTAGRLRSVP
Mvan_0539|M.vanbaalenii_PYR-1 RVLLNKCRHRGNKVCQFDK------GNANIFHCSYHGWSYDTAGRLRSVP
Mb3186c|M.bovis_AF2122/97 HALSPICRHRLMLMVEPGA------GRIDTLTCQYHLWRYGLDGRLRGAP
Rv3161c|M.tuberculosis_H37Rv HALSPICRHRLMLMVEPGA------GRIDTLTCQYHLWRYGLDGRLRGAP
MAB_4370c|M.abscessus_ATCC_199 RAFLNSCRHRGARVCRADY------GVTRGFTCTYHGWAYDLEGKLISVP
MAV_3509|M.avium_104 RALFNMCLHRGMQVCRAEL------GNASNFRCPYHGWTYRNDGRLTGLP
MMAR_4744|M.marinum_M RAFHNICRHRGNKLVWNDFPHEETAGTCRQFTCKYHAWRYGLDGELTFIQ
TH_2976|M.thermoresistible__bu HLLLNRCSHRGNQVCADAK------GNSSTFRCPYHGWTFRNTGELVGFP
MSMEG_1908|M.smegmatis_MC2_155 HCLINACAHRGAMICRRKT------DNRTTLTCPFHGWTFRNDGTLLKVK
MUL_3820|M.ulcerans_Agy99 RAFDNVCLHRQSRVATGCG-------TALRFTCPYHGWTYDNGGRLVGLP
: : * ** : : * :* * : * *
Mflv_0626|M.gilvum_PYR-GCK LAESAYG--PQFDKASMGLVSP-RVATYKGLIFACWDQSA-PPLEEYLGE
Mvan_0539|M.vanbaalenii_PYR-1 LAESAYG--PQFDKASMGLVSP-RVATYKGLIFACWDQSA-PPLEEYLGE
Mb3186c|M.bovis_AF2122/97 HMAAN----LDFNRRECRLPQF-AVATWNGLVWINLDADA-EPIAAHL--
Rv3161c|M.tuberculosis_H37Rv HMAAN----LDFNRRECRLPQF-AVATWNGLVWINLDADA-EPIAAHL--
MAB_4370c|M.abscessus_ATCC_199 NEEGYP---DSFSTDDWGLVEVPHVDSYHGLIFGTWNPAP-VGLRESLG-
MAV_3509|M.avium_104 FHREAYGGDDGFVKDQTLLPAP-NFAGYNGLLFISLDPAA-PPLQDFLG-
MMAR_4744|M.marinum_M QEDEFFD----VDKSQYGLKSV-RCEVWEGFIFINLDPAA-APLSEYLG-
TH_2976|M.thermoresistible__bu FFKGYG-----TRKLDLPLGRVPRVDSYGGFVFGSFATEG-PTLREHLG-
MSMEG_1908|M.smegmatis_MC2_155 DPDGAGYPDTFNSNGSHNMTNVARFEGYRGFLFGSLNPDV-LPLTEHLG-
MUL_3820|M.ulcerans_Agy99 GREGFPG----IAVGSDGLTEL-PATEFAGFLWVSLHPAARLDVPAHLG-
. : : *::: : *
Mflv_0626|M.gilvum_PYR-GCK GLLWYLDNFLLDCDPNGLQVVPG-LHRYLMPVNWKLLAENFGGDQYHFAA
Mvan_0539|M.vanbaalenii_PYR-1 GLLWYLDNFLLDCDPNGLQVVPG-LHRYLMPVNWKLLAENFGGDQYHFAA
Mb3186c|M.bovis_AF2122/97 ----DLTDDEFAGYRLGEMVQVE-SWSHEWRANWKVAAEN-GHENYHVLG
Rv3161c|M.tuberculosis_H37Rv ----DLTDDEFAGYRLGEMVQVE-SWSHEWRANWKVAAEN-GHENYHVLG
MAB_4370c|M.abscessus_ATCC_199 -DMAWYMDAMLDHDSEGTVVVGG-VHKWVLDGNWKLAAEQFATDWYHVNM
MAV_3509|M.avium_104 --DFRFYLDYYTRQSAGGVELRG-PQRWRIRANWKIGAENFAGDMYHTPH
MMAR_4744|M.marinum_M -----SMAKGLQGYPFHEMTEVY-SYRAEVGSNWKLFIDAFAEFYHAPVL
TH_2976|M.thermoresistible__bu -AAAGEIDRLLRLSPEGKVALDAGWLRHRTRANWKLLVEN-ETDGYHPQF
MSMEG_1908|M.smegmatis_MC2_155 -DTTTVIDMLVDQSPDGLEVLRG-ASTYTYDGNWKVQAEN-GADGYHVTA
MUL_3820|M.ulcerans_Agy99 -----PLAGELQSWGIGRWSPLG-EKVLGSPIDWKLATDTFAENYHFATV
:**: : :
Mflv_0626|M.gilvum_PYR-GCK THGSVSALSKAGQTARINFSIDEGQHYSVVLDGCAPHGLLQLAVGKNFYQ
Mvan_0539|M.vanbaalenii_PYR-1 THGSVSALSKAGQTARINFSIDEGQHYSVVLDGCAPHGLLQLAVGKNFYQ
Mb3186c|M.bovis_AF2122/97 LHRQTLEPFVPGGGD---LDVRQYSRWALRLR------------------
Rv3161c|M.tuberculosis_H37Rv LHRQTLEPFVPGGGD---LDVRQYSRWALRLR------------------
MAB_4370c|M.abscessus_ATCC_199 SHASALMVMAPQGKAPKDEIVHRTGRQYVDPNGHGAG-------------
MAV_3509|M.avium_104 THASIVDIGLFREPK---AQKRKDGATYWAHRGGGTTYKLPPGGFEERMR
MMAR_4744|M.marinum_M HQGQYTKEEAAKIMKYGFEALHYELAGPHAMISVWGG-------------
TH_2976|M.thermoresistible__bu VHGGIFGVTGSTIGP---LYSDSSTAVTRDLGGGHSE-------------
MSMEG_1908|M.smegmatis_MC2_155 THWNYAATTSRRTCGESKNDTKALDAGSWGKSGGGYWS------------
MUL_3820|M.ulcerans_Agy99 HRRTFATIARSNCTVFDSYGPHHR--------------------------
:
Mflv_0626|M.gilvum_PYR-GCK DDLAQAETLGTEAVDWLTERQRLQDE--RLATSPVQPYS-FHVANIFPN-
Mvan_0539|M.vanbaalenii_PYR-1 DDLAQAETLGTEAVDWLTERQRLQDE--RLATSPVQPYS-FHVANIFPN-
Mb3186c|M.bovis_AF2122/97 ----------------VPFTVPVEAK--SLQLNEVQKSN-LVVLWTFPNS
Rv3161c|M.tuberculosis_H37Rv ----------------VPFTVPVEAK--SLQLNEVQKSN-LVVLWTFPNS
MAB_4370c|M.abscessus_ATCC_199 FPVHPKNRFDARVVHQWTDYGALRER--LGEARVQGPLT-NGHATVFPNL
MAV_3509|M.avium_104 YVGYPDEMIGRIKQVWTPRQQRVVGEDGFMVSAATCFPN-LSFVHNWPKV
MMAR_4744|M.marinum_M -------------QSPPADLKMVKPMDRVLRSGLFGPWDRPAPITDLEEL
TH_2976|M.thermoresistible__bu ---NDLRPEFRKFAEPLRWFGTTESRVPDYVAAMRARYGAEADQILIEGS
MSMEG_1908|M.smegmatis_MC2_155 ---FPHGHLCLWTWAANPQDRPLWDRLDELKARFGDAKG-EFMVKGSRNL
MUL_3820|M.ulcerans_Agy99 ---------------LIFPLNTILELENVPEGQWDPFHHMVVIYALFPNV
Mflv_0626|M.gilvum_PYR-GCK -FSMIGMGTAFYGRGFIMWQPRGPRLTEVWEWCLVESSAPRAVKERMVFV
Mvan_0539|M.vanbaalenii_PYR-1 -FSMIGMGTAFYGRGFIMWQPRGPRLTEVWEWCLVESSAPRAVKERMVFV
Mb3186c|M.bovis_AF2122/97 ALAIAGERVVWFG-----FIPQSIDRVQVLGGVLTTP----ELAADAAAT
Rv3161c|M.tuberculosis_H37Rv ALAIAGERVVWFG-----FIPQSIDRVQVLGGVLTTP----ELAADAAAT
MAB_4370c|M.abscessus_ATCC_199 SYLPVNGSVRIWH-------PKGPDKMEVWAWTIVDRSMPEEVRDAQRLY
MAV_3509|M.avium_104 RDGRDDETLPFISIR--LWQPISENETEVCSWFAVDSAAPAHYKQDSYKA
MMAR_4744|M.marinum_M PPGINPTKAKQWGIDSFLFFPNFMLLIWEPGWYLTYHYWPTTVDKHIFET
TH_2976|M.thermoresistible__bu PHVMIFPNLFIAEIQVFNIQPVAVNECVQYSTAVQLVGAP-ELNRRMVSQ
MSMEG_1908|M.smegmatis_MC2_155 CLYPNVYVMDQFSTQIRHFRPIAPDKTEVTIYCIAPKGESAAARAHRIRQ
MUL_3820|M.ulcerans_Agy99 VLSVTIANGELFR--------IYPGDRPGSSITVHQNSTPLDLSDESVAT
Mflv_0626|M.gilvum_PYR-GCK LSQRQS--AAGLVTPDDHENFERLSDALDTGVARDVPFNYSLGED-----
Mvan_0539|M.vanbaalenii_PYR-1 LSQRQS--AAGLVTPDDHENFERLSDALDTGVARDVPFNYSLGED-----
Mb3186c|M.bovis_AF2122/97 AQTSQF--VMAMINDEDRLGLEAVQVGAGSRFAERGHLSS----------
Rv3161c|M.tuberculosis_H37Rv AQTSQF--VMAMINDEDRLGLEAVQVGAGSRFAERGHLSS----------
MAB_4370c|M.abscessus_ATCC_199 NLRTFG--PTGIFEQDDGENWSEVQSISRGFVTNSVALNYQMGLD-----
MAV_3509|M.avium_104 YLMCFG--STGMFEQDDVENWVSLTTTAGGSMARRLLLNSRMGLYDDGRP
MMAR_4744|M.marinum_M TLYFVP--PRNARERLAQELAAVTFKEYALQDSNTLEATQTMIGTG----
TH_2976|M.thermoresistible__bu CIGSVG--PAGMLLADDTEMYERNQQGLEALTPEWLDIRRGLNREH----
MSMEG_1908|M.smegmatis_MC2_155 YEDFFN--ASGMATPDDLEEFRSCQLTFRAQAAPWNDMSRGAQHWLSG-P
MUL_3820|M.ulcerans_Agy99 GAQAVFDYAHATVRDEDYRLAETLQTNLESGARQRLVFGR----------
Mflv_0626|M.gilvum_PYR-GCK -VEPMESLVAELPGNVRPQISEAYQREFYRHWHRTMTEPA-------
Mvan_0539|M.vanbaalenii_PYR-1 -VEPMESLVAELPGNVRPQISEAYQREFYRHWHRTMTEPA-------
Mb3186c|M.bovis_AF2122/97 ---------KEWPG------MLAFYRNLAMALVGDHPGAS-------
Rv3161c|M.tuberculosis_H37Rv ---------KEWPG------MLAFYRNLAMALVGDHPGAS-------
MAB_4370c|M.abscessus_ATCC_199 -SERED---GRHPGLTSELYSDAAGRSFYARWRDLMNTKAWHEEHV-
MAV_3509|M.avium_104 VVEALAPSAFHGPGRAQVGYNEHNQRALLAMWADYLQEGDACENR--
MMAR_4744|M.marinum_M -------VVKDFPLCDQEILLRHLHKMTGDYVKEYRNNGHAK-----
TH_2976|M.thermoresistible__bu --------VDDHGFTVGGPTDETGMRAFWSEYRRLMEAE--------
MSMEG_1908|M.smegmatis_MC2_155 DPVAESLGMHGIVSSGLRNEDEGLYPVQHGYWLETLRRAVTSNPTEH
MUL_3820|M.ulcerans_Agy99 --------------------NEPGLQHRHITWAQALETPG-------