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ETIGSKTGSALPIDSSAFYDEAVYQRELDSIFKRSWLFVGHESMIPKPGDFRTTYMADDAVIVCRDKESR VRVLLNKCRHRGNKVCQFDKGNANIFHCSYHGWSYDTAGRLRSVPLAESAYGPQFDKASMGLVSPRVATY KGLIFACWDQSAPPLEEYLGEGLLWYLDNFLLDCDPNGLQVVPGLHRYLMPVNWKLLAENFGGDQYHFAA THGSVSALSKAGQTARINFSIDEGQHYSVVLDGCAPHGLLQLAVGKNFYQDDLAQAETLGTEAVDWLTER QRLQDERLATSPVQPYSFHVANIFPNFSMIGMGTAFYGRGFIMWQPRGPRLTEVWEWCLVESSAPRAVKE RMVFVLSQRQSAAGLVTPDDHENFERLSDALDTGVARDVPFNYSLGEDVEPMESLVAELPGNVRPQISEA YQREFYRHWHRTMTEPA*
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. gilvum PYR-GCK | Mflv_0544 | - | - | 100% (438) | Rieske (2Fe-2S) domain-containing protein |
| M. gilvum PYR-GCK | Mflv_0626 | - | 0.0 | 99.77% (438) | Rieske (2Fe-2S) domain-containing protein |
| M. gilvum PYR-GCK | Mflv_0632 | - | 1e-65 | 35.19% (432) | ring hydroxylating dioxygenase, alpha subunit |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb3186c | - | 1e-20 | 31.10% (209) | dioxygenase |
| M. tuberculosis H37Rv | Rv3161c | - | 8e-21 | 31.10% (209) | dioxygenase |
| M. leprae Br4923 | - | - | - | - | - |
| M. abscessus ATCC 19977 | MAB_4370c | - | 3e-61 | 34.66% (427) | putative large terminal subunit of phenylpropionate dioxygenase |
| M. marinum M | MMAR_4744 | - | 5e-22 | 29.34% (259) | dioxygenase |
| M. avium 104 | MAV_3509 | - | 2e-47 | 31.19% (436) | phthalate dioxygenase large subunit |
| M. smegmatis MC2 155 | MSMEG_1908 | - | 1e-33 | 27.49% (371) | benzoate 1,2-dioxygenase alpha subunit |
| M. thermoresistible (build 8) | TH_2976 | - | 2e-32 | 37.25% (204) | PUTATIVE dioxygenase large subunit |
| M. ulcerans Agy99 | MUL_3820 | - | 6e-14 | 26.34% (243) | ring-hydroxylating dioxygenase, large terminal subunit |
| M. vanbaalenii PYR-1 | Mvan_0539 | - | 0.0 | 100.00% (438) | Rieske (2Fe-2S) domain-containing protein |
CLUSTAL 2.0.9 multiple sequence alignment
Mflv_0544|M.gilvum_PYR-GCK ------------------------METIGSKTGSALPIDSSAFYDEAVYQ
Mvan_0539|M.vanbaalenii_PYR-1 ------------------------METIGSKTGSALPIDSSAFYDEAVYQ
MAB_4370c|M.abscessus_ATCC_199 MASLPRPVAVAQGALCRPQALVRDIDVSGLVDSHGGLLDRVIFTDGELYR
Mb3186c|M.bovis_AF2122/97 --------MLSTDNRAELGDILTDIGDYLDDNPPALSLPPAAYTSSELWQ
Rv3161c|M.tuberculosis_H37Rv --------MLSTDNRAELGDILTDIGDYLDDNPPALSLPPAAYTSSELWQ
MAV_3509|M.avium_104 ------------------MAGPDGGLLGVLENVRRGMIPAHIYNDPELFA
MMAR_4744|M.marinum_M --------------MPHFTKPAAGSWTQNYPELGTAPVDYTDSIDPQYFA
TH_2976|M.thermoresistible__bu -------------------------------LIRPDRVHGSLYTDPAIFA
MSMEG_1908|M.smegmatis_MC2_155 ---------MTTHLSPDHLENVLADAVIEDPAAGVYRANRRIFTDDEIFE
MUL_3820|M.ulcerans_Agy99 -------MDRDQLIDLTRRALKLARDKTTDLAPHQHAVDAREYTSLDRHE
.
Mflv_0544|M.gilvum_PYR-GCK RELDSIFKRSWLFVGHESMIPKPGDFRTTYMADD----AVIVCRDKESRV
Mvan_0539|M.vanbaalenii_PYR-1 RELDSIFKRSWLFVGHESMIPKPGDFRTTYMADD----AVIVCRDKESRV
MAB_4370c|M.abscessus_ATCC_199 QELRRVFAPSWLFLAHADQFHRPGDFFTTYMGED----PVIVSMDKNRRI
Mb3186c|M.bovis_AF2122/97 LERERIFNRSWMLVAHVDQLAKTGDYVTVSVAGE----PVMVVRDVDGQL
Rv3161c|M.tuberculosis_H37Rv LERERIFNRSWMLVAHVDQVAKTGDYVTVSVAGE----PVMVVRDVDGQL
MAV_3509|M.avium_104 LEKRRLFARAWTFVGHESEIPHDGDYMVRRVLDD----SFIITRGSDGRV
MMAR_4744|M.marinum_M DEQRAIFRRTWLNVGRIERLPRTGSYFTKELPSTGAGMSVIVVKGKDGVI
TH_2976|M.thermoresistible__bu EELRKIWYRTWVFVGHESEVSQPNDYVRKKLGLQ----DVIMTRDRDGEI
MSMEG_1908|M.smegmatis_MC2_155 LEMKHIFEGNWVYLAHESQVPNPGDYFTTYIGRQ----PVVITRDRDGDL
MUL_3820|M.ulcerans_Agy99 RDRAMVLD-SPQLVGYSSELPGPDTYRTKTIMGR----SILLTRAADRAV
: : :. . . . : : .:: : :
Mflv_0544|M.gilvum_PYR-GCK RVLLNKCRHRGNKVCQFDK------GNANIFHCSYHGWSYDTAGRLRSVP
Mvan_0539|M.vanbaalenii_PYR-1 RVLLNKCRHRGNKVCQFDK------GNANIFHCSYHGWSYDTAGRLRSVP
MAB_4370c|M.abscessus_ATCC_199 RAFLNSCRHRGARVCRADY------GVTRGFTCTYHGWAYDLEGKLISVP
Mb3186c|M.bovis_AF2122/97 HALSPICRHRLMLMVEPGA------GRIDTLTCQYHLWRYGLDGRLRGAP
Rv3161c|M.tuberculosis_H37Rv HALSPICRHRLMLMVEPGA------GRIDTLTCQYHLWRYGLDGRLRGAP
MAV_3509|M.avium_104 RALFNMCLHRGMQVCRAEL------GNASNFRCPYHGWTYRNDGRLTGLP
MMAR_4744|M.marinum_M RAFHNICRHRGNKLVWNDFPHEETAGTCRQFTCKYHAWRYGLDGELTFIQ
TH_2976|M.thermoresistible__bu HLLLNRCSHRGNQVCADAK------GNSSTFRCPYHGWTFRNTGELVGFP
MSMEG_1908|M.smegmatis_MC2_155 HCLINACAHRGAMICRRKT------DNRTTLTCPFHGWTFRNDGTLLKVK
MUL_3820|M.ulcerans_Agy99 RAFDNVCLHRQSRVATGCG-------TALRFTCPYHGWTYDNGGRLVGLP
: : * ** : : * :* * : * *
Mflv_0544|M.gilvum_PYR-GCK -LAESAYGPQF-DKASMGLVS-PRVATYKGLIFACWDQSA-PPLEEYLGE
Mvan_0539|M.vanbaalenii_PYR-1 -LAESAYGPQF-DKASMGLVS-PRVATYKGLIFACWDQSA-PPLEEYLGE
MAB_4370c|M.abscessus_ATCC_199 -NEE-GYPDSF-STDDWGLVEVPHVDSYHGLIFGTWNPAP-VGLRESLGD
Mb3186c|M.bovis_AF2122/97 -HMAANLDFNR-RECRLPQFA---VATWNGLVWINLDADA-EPIAAHLDL
Rv3161c|M.tuberculosis_H37Rv -HMAANLDFNR-RECRLPQFA---VATWNGLVWINLDADA-EPIAAHLDL
MAV_3509|M.avium_104 -FHREAYGGDDGFVKDQTLLPAPNFAGYNGLLFISLDPAA-PPLQDFLGD
MMAR_4744|M.marinum_M -QEDEFFDVDK-SQYGLKSVR---CEVWEGFIFINLDPAA-APLSEYLGS
TH_2976|M.thermoresistible__bu -FFK-GYGTRK---LDLPLGRVPRVDSYGGFVFGSFATEG-PTLREHLGA
MSMEG_1908|M.smegmatis_MC2_155 DPDGAGYPDTFNSNGSHNMTNVARFEGYRGFLFGSLNPDV-LPLTEHLGD
MUL_3820|M.ulcerans_Agy99 --GREGFPGIAVGSDGLTELP---ATEFAGFLWVSLHPAARLDVPAHLGP
: *::: : *.
Mflv_0544|M.gilvum_PYR-GCK GLLWYLDNFLLDCDPNGLQVVPG-LHRYLMPVNWKLLAENFGGDQYHFAA
Mvan_0539|M.vanbaalenii_PYR-1 GLLWYLDNFLLDCDPNGLQVVPG-LHRYLMPVNWKLLAENFGGDQYHFAA
MAB_4370c|M.abscessus_ATCC_199 -MAWYMD-AMLDHDSEGTVVVGG-VHKWVLDGNWKLAAEQFATDWYHVNM
Mb3186c|M.bovis_AF2122/97 TDDEFAG------YRLGEMVQVE-SWSHEWRANWKVAAEN-GHENYHVLG
Rv3161c|M.tuberculosis_H37Rv TDDEFAG------YRLGEMVQVE-SWSHEWRANWKVAAEN-GHENYHVLG
MAV_3509|M.avium_104 -FRFYLD--YYTRQSAGGVELRG-PQRWRIRANWKIGAENFAGDMYHTPH
MMAR_4744|M.marinum_M MAKGLQG------YPFHEMTEVY-SYRAEVGSNWKLFIDAFAEFYHAPVL
TH_2976|M.thermoresistible__bu AAGEIDR--LLRLSPEGKVALDAGWLRHRTRANWKLLVEN-ETDGYHPQF
MSMEG_1908|M.smegmatis_MC2_155 TTTVIDM--LVDQSPDGLEVLRG-ASTYTYDGNWKVQAEN-GADGYHVTA
MUL_3820|M.ulcerans_Agy99 LAGELQSWGIGRWSPLGEKVLGS-------PIDWKLATDTFAENYHFATV
:**: : :
Mflv_0544|M.gilvum_PYR-GCK THGSVSALSKAGQTARINFSIDEGQHYSVVLDGCAPHGLLQLAVGKNFYQ
Mvan_0539|M.vanbaalenii_PYR-1 THGSVSALSKAGQTARINFSIDEGQHYSVVLDGCAPHGLLQLAVGKNFYQ
MAB_4370c|M.abscessus_ATCC_199 SHASALMVMAPQGKAPKDEIVHRTGRQYVDPNGHGAG--FPVHPKNRFDA
Mb3186c|M.bovis_AF2122/97 LHRQTLEPFVPGG---GDLDVRQYSRWALRLR-------VPFTVPVEAKS
Rv3161c|M.tuberculosis_H37Rv LHRQTLEPFVPGG---GDLDVRQYSRWALRLR-------VPFTVPVEAKS
MAV_3509|M.avium_104 THASIVDIGLFRE---PKAQKRKDGATYWAHRGGGTTYKLPPGGFEERMR
MMAR_4744|M.marinum_M HQGQYTKEEAAKIMKYGFEALHYELAGPHAMISVWGGQSPPADLKMVKPM
TH_2976|M.thermoresistible__bu VHGGIFGVTGSTIG---PLYSDSSTAVTRDLGGGHSENDLRPEFRKFAEP
MSMEG_1908|M.smegmatis_MC2_155 THWNYAATTSRRTCGESKNDTKALDAGSWGKSGGGYWSFPHGHLCLWTWA
MUL_3820|M.ulcerans_Agy99 HRRTFATIARSNCTVFDSYGPHHR-------------LIFPLNTILELEN
:
Mflv_0544|M.gilvum_PYR-GCK DDLAQAETLGTEAVDWLTERQRLQDERLATSPVQPYSFHVANIFPNFSMI
Mvan_0539|M.vanbaalenii_PYR-1 DDLAQAETLGTEAVDWLTERQRLQDERLATSPVQPYSFHVANIFPNFSMI
MAB_4370c|M.abscessus_ATCC_199 RVVHQWTDYG-------ALRERLGEARVQG----PLTNGHATVFPNLSYL
Mb3186c|M.bovis_AF2122/97 LQLNEVQKSN---------------------------LVVLWTFPNSALA
Rv3161c|M.tuberculosis_H37Rv LQLNEVQKSN---------------------------LVVLWTFPNSALA
MAV_3509|M.avium_104 YVGYPDEMIG-------RIKQVWTPRQQRVVGEDGFMVSAATCFPNLSFV
MMAR_4744|M.marinum_M DRVLRSGLFGPWDRPAPITDLEELPPGINPTKAKQWGIDSFLFFPNFMLL
TH_2976|M.thermoresistible__bu LRWFGTTES----------RVPDYVAAMRARYGAEADQILIEGSPHVMIF
MSMEG_1908|M.smegmatis_MC2_155 ANPQDRPLWDR-------------LDELKARFGDAKGEFMVKGSRNLCLY
MUL_3820|M.ulcerans_Agy99 VPEGQWDPFH-------------------------HMVVIYALFPNVVLS
:
Mflv_0544|M.gilvum_PYR-GCK G---------MGTAFYGRGFIMWQPRGPRLTEVWEWCLVESSAPRAVKER
Mvan_0539|M.vanbaalenii_PYR-1 G---------MGTAFYGRGFIMWQPRGPRLTEVWEWCLVESSAPRAVKER
MAB_4370c|M.abscessus_ATCC_199 P---------VNGSVR-----IWHPKGPDKMEVWAWTIVDRSMPEEVRDA
Mb3186c|M.bovis_AF2122/97 I---------AGERVVWFG---FIPQSIDRVQVLGGVLTTP----ELAAD
Rv3161c|M.tuberculosis_H37Rv I---------AGERVVWFG---FIPQSIDRVQVLGGVLTTP----ELAAD
MAV_3509|M.avium_104 HNWPKVRDGRDDETLPFISIRLWQPISENETEVCSWFAVDSAAPAHYKQD
MMAR_4744|M.marinum_M I----------WEPGWYLTYHYWPTTVDKHIFETTLYFVPP-----RNAR
TH_2976|M.thermoresistible__bu P----------NLFIAEIQVFNIQPVAVNECVQYSTAVQLVGAP-ELNRR
MSMEG_1908|M.smegmatis_MC2_155 PN-------VYVMDQFSTQIRHFRPIAPDKTEVTIYCIAPKGESAAARAH
MUL_3820|M.ulcerans_Agy99 VT---------------IANGELFRIYPGDRPGSSITVHQNSTPLDLSDE
Mflv_0544|M.gilvum_PYR-GCK MVFVLSQRQSAAGLVTPDDHENFERLSDALDTGVARDVPFNYSLGEDV--
Mvan_0539|M.vanbaalenii_PYR-1 MVFVLSQRQSAAGLVTPDDHENFERLSDALDTGVARDVPFNYSLGEDV--
MAB_4370c|M.abscessus_ATCC_199 QRLYNLRTFGPTGIFEQDDGENWSEVQSISRGFVTNSVALNYQMGLDS--
Mb3186c|M.bovis_AF2122/97 AAATAQTSQFVMAMINDEDRLGLEAVQVGAGSRFAERGHLSSKEWPG---
Rv3161c|M.tuberculosis_H37Rv AAATAQTSQFVMAMINDEDRLGLEAVQVGAGSRFAERGHLSSKEWPG---
MAV_3509|M.avium_104 SYKAYLMCFGSTGMFEQDDVENWVSLTTTAGGSMARRLLLNSRMGLYDDG
MMAR_4744|M.marinum_M ERLAQELAAVTFKEYALQDSNTLEATQTMIGTGVVKDFPLCDQEILLR--
TH_2976|M.thermoresistible__bu MVSQCIGSVGPAGMLLADDTEMYERNQQGLEALTPEWLDIRRGLNREH--
MSMEG_1908|M.smegmatis_MC2_155 RIRQYEDFFNASGMATPDDLEEFRSCQLTFRAQAAPWNDMSRGAQHWLSG
MUL_3820|M.ulcerans_Agy99 SVATGAQAVFDYAHATVRDEDYRLAETLQTNLESGARQRLVFGRNEPG--
* :
Mflv_0544|M.gilvum_PYR-GCK ----EPMESLVAELPGNVRPQISEAYQREFYRH--WHRTMTEPA------
Mvan_0539|M.vanbaalenii_PYR-1 ----EPMESLVAELPGNVRPQISEAYQREFYRH--WHRTMTEPA------
MAB_4370c|M.abscessus_ATCC_199 ----ERED---GRHPGLTSELYSDAAGRSFYAR--WRDLMNTKAWHEEHV
Mb3186c|M.bovis_AF2122/97 ---------MLAFYRNLAMALVGDHPGAS---------------------
Rv3161c|M.tuberculosis_H37Rv ---------MLAFYRNLAMALVGDHPGAS---------------------
MAV_3509|M.avium_104 RPVVEALAPSAFHGPGRAQVGYNEHNQRALLAM--WADYLQEGDACENR-
MMAR_4744|M.marinum_M ---------HLHKMTGDYVKEYRNNGHAK---------------------
TH_2976|M.thermoresistible__bu ----------VDDHGFTVGGPTDETGMRAFWSE--YRRLMEAE-------
MSMEG_1908|M.smegmatis_MC2_155 P---DPVAESLGMHGIVSSGLRNEDEGLYPVQHGYWLETLRRAVTSNPTE
MUL_3820|M.ulcerans_Agy99 -----------LQHRHITWAQALETPG-----------------------
:
Mflv_0544|M.gilvum_PYR-GCK -
Mvan_0539|M.vanbaalenii_PYR-1 -
MAB_4370c|M.abscessus_ATCC_199 -
Mb3186c|M.bovis_AF2122/97 -
Rv3161c|M.tuberculosis_H37Rv -
MAV_3509|M.avium_104 -
MMAR_4744|M.marinum_M -
TH_2976|M.thermoresistible__bu -
MSMEG_1908|M.smegmatis_MC2_155 H
MUL_3820|M.ulcerans_Agy99 -