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KDKKFHLGWFMNFTSDLWTGPFEQAGGAPWDGQFYVEMAKNLERACFDFIMMEDKLAVSEAYGGTKETVL KHAFGMVPKHDPAPLATLISASTQHLGVVATLSTLGYPPFLLARLCSTIDHISHGRFGWNIVTSAENAAA QNFGLDELPPREDRYAMADEYMDLVYQLWDSWEPDAVVIDRERGVHTDFTKVHEINFHGKYYKCRGPLNT APSPQGRPTFLQAGGSPRGRDFAAKHSDAIITLADGVDGMKAYREDIRGRAAAQGRNPDEIKVMYLVIPT LGETTEEAVARRDRLTTSQAFAEQTLSLLSSITDIDFSQFDLDSTLPHLTTNGEQGSLDKFQQSGSGKTL RELIYDSADFCSSYPLCGTPSSVADEMEWLMDEVGGDGFLITLHNQGVSRRHVIEVTDGLIPELQGRGVV RTEYTQNTLRETLREF*
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. smegmatis MC2 155 | MSMEG_6734 | - | - | 100% (437) | dibenzothiophene desulfurization enzyme A |
| M. smegmatis MC2 155 | MSMEG_4389 | - | 3e-68 | 36.43% (431) | monooxygenase, NtaA/SnaA/SoxA family protein |
| M. smegmatis MC2 155 | MSMEG_1162 | - | 1e-67 | 37.44% (406) | nitrilotriacetate monooxygenase component A |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb0815c | - | 3e-05 | 20.53% (190) | hypothetical protein Mb0815c |
| M. gilvum PYR-GCK | Mflv_0250 | - | 3e-80 | 37.56% (442) | hypothetical protein Mflv_0250 |
| M. tuberculosis H37Rv | Rv0791c | - | 3e-05 | 20.53% (190) | hypothetical protein Rv0791c |
| M. leprae Br4923 | - | - | - | - | - |
| M. abscessus ATCC 19977 | MAB_2482 | - | 1e-63 | 36.58% (421) | monooxygenase |
| M. marinum M | MMAR_0703 | - | 2e-57 | 30.32% (432) | FMNH2-utilizing oxygenase |
| M. avium 104 | MAV_0340 | - | 2e-68 | 35.06% (405) | nitrilotriacetate monooxygenase component A |
| M. thermoresistible (build 8) | TH_4268 | - | 2e-58 | 32.78% (424) | PUTATIVE putative monooxygenase |
| M. ulcerans Agy99 | MUL_4507 | fgd2 | 7e-07 | 23.92% (209) | F420-dependent glucose-6-phosphate dehydrogenase Fgd2 |
| M. vanbaalenii PYR-1 | Mvan_6020 | - | 2e-54 | 32.67% (404) | xenobiotic compound DszA family monooxygenase |
CLUSTAL 2.0.9 multiple sequence alignment
MSMEG_6734|M.smegmatis_MC2_155 ----------MKD---KKFHLG-----WFMNFTSDLWTGPFEQAGGAPWD
Mflv_0250|M.gilvum_PYR-GCK ----------MTDSAKRKAHLLGFVQHGVMNHASTMWAHPRDKVG-YHWS
MAB_2482|M.abscessus_ATCC_1997 MCGTRNLPGPDRRFPVSRIYLNAFDMACVGHQSPGLWRHPDDQGH--RYK
TH_4268|M.thermoresistible__bu -------------------------MNCVAHQSPGLWKHPEDQSW--RYK
MAV_0340|M.avium_104 -------------MRTDKMALVAFMQAGSTSVYAGSWRHPANEHR---YL
MMAR_0703|M.marinum_M ----------------MIRKPVHLAAHFPGVNNTTVWADPGAGSQ----I
Mvan_6020|M.vanbaalenii_PYR-1 -----------------MMHLITWLMGNSYHAAG--WRHQLAWER--TAM
Mb0815c|M.bovis_AF2122/97 ------------MNAKDDPHFGLMLAATVNGLAVGSYREMVVVSQTAEEY
Rv0791c|M.tuberculosis_H37Rv ------------MNAKDDPHFGLMLAATVNGLAVGSYREMVVVSQTAEEY
MUL_4507|M.ulcerans_Agy99 --------------MALLFHWFLPTYGDSRGLVAGGHGLPMSGNR---PA
MSMEG_6734|M.smegmatis_MC2_155 G-QFYVEMAKNLERACFDFIMMEDKLAVSEAYGGTKETVLKHAF--GMVP
Mflv_0250|M.gilvum_PYR-GCK RPEYWRDLGRTMERGLFDAMFIADELAPYTTYKGSSDPVVKFA---GQCP
MAB_2482|M.abscessus_ATCC_1997 SLGYWTDLAKTLEAGGFDLLFLADVLGAYDVYGGSRDAAVADA---AQFP
TH_4268|M.thermoresistible__bu DLEYWTELARLLERGRFDGLFIADVLGTYDVYGAGDEAAIRQA---AQIP
MAV_0340|M.avium_104 DAAYYAKIGRQLEEGCFDLMFFDDRLAMPGVYGGSVGEAVRYG---ARPV
MMAR_0703|M.marinum_M EFESFVHLARTAERGLFDFFFLAEGLRLREHRGRIHDLDVVGR-------
Mvan_6020|M.vanbaalenii_PYR-1 RLDVLIEIAKIAEEAKLDALFVADGNGVRNMDKVGLFEANTPS---ARPT
Mb0815c|M.bovis_AF2122/97 GFDSVWLCDHFLTISPGEYAKVAGIAADTGSATGTETGGAGQCAPSRSLP
Rv0791c|M.tuberculosis_H37Rv GFDSVWLCDHFLTISPGEYAKVAGIAADTGSATGTETGGAGQCAPSRSLP
MUL_4507|M.ulcerans_Agy99 TLRYLNQIAAAAESNGFEAVLTPAG-----------------------LW
:
MSMEG_6734|M.smegmatis_MC2_155 KHDPAPLATLISASTQHLGVV-ATLSTLGYPPFLLARLCSTIDHISHGRF
Mflv_0250|M.gilvum_PYR-GCK VHEPASVVPIVGAFTKHLGIG-ITLSTSFVPPYMMARQLSTLDHLTGGRV
MAB_2482|M.abscessus_ATCC_1997 VNDPTLAVSAMAAVTETLGFG-LTLSLTYEQPYALARRLTTLDHLTEGRV
TH_4268|M.thermoresistible__bu VNDPMMLVSAMALVTEHLGFG-ITTGTGFEHPYPFARRMSTLDHLTKGRI
MAV_0340|M.avium_104 KLDLGVILGVLAQATSRIGLG-ATYSTTYYPPFHVARTFASLDHLSGGRA
MMAR_0703|M.marinum_M -PDTFTLLAALAGVTDRIGLT-GTINTTFNEPFEVARQFATLDHLSEGRA
Mvan_6020|M.vanbaalenii_PYR-1 VYDPVTLMAAISQHTKHIGLV-GTASTTYESPWVVARRFASLDHLSNGRA
Mb0815c|M.bovis_AF2122/97 LLECWTALAALSRDTTKLRLGTSVLCNSYRHPSVLAKMAATLDVISQGRL
Rv0791c|M.tuberculosis_H37Rv LLECWTALAALSRDTTKLRLGTSVLCNSYRHPSVLAKMAATLDVISQGRL
MUL_4507|M.ulcerans_Agy99 CEDAWLTTAMLVGTTKTLKFL-VALRPGLVSPTLAAQMATTFQQQSGGRL
: : * : . . * *: :::: : **
MSMEG_6734|M.smegmatis_MC2_155 GWNIVTSAENAAAQNFGLDELPPREDRYAMADEYMDLVYQLWD-SWEPDA
Mflv_0250|M.gilvum_PYR-GCK GWNIVTSYSKSEFQAMGKENLTPRDKRYEVVEEYMQLLYQLWD-SWDDDA
MAB_2482|M.abscessus_ATCC_1997 AWNIVTSYLDSAARNLGLDAQIPHDQRYDIAEEYLQVCYKLWEGSWESGA
TH_4268|M.thermoresistible__bu GWNVVTGYLPAAARNMGQSDQPAHDARYDHADEYLEVLYKLWEGSWEDDA
MAV_0340|M.avium_104 AWNVVTSVNDSEAQNFGVDAHLGHDERYDRADEFMDVVTGLWD-TWEDDA
MMAR_0703|M.marinum_M GWNIVTSSDAFTGANFRRGGYLDHADRYQRAEEFLTVARKFWD-SWAPGA
Mvan_6020|M.vanbaalenii_PYR-1 SWNVVTGSNPGDSENFGLAHHPDRDSRYARAEEFVSACKALWN-SWDEDA
Mb0815c|M.bovis_AF2122/97 DLGLGAGWFRRESQAYGIP-FPPVGDRVSALAESLQVIKAVWT-------
Rv0791c|M.tuberculosis_H37Rv DLGLGAGWFRRESQAYGIP-FPPVGDRVSALAESLQVIKAVWT-------
MUL_4507|M.ulcerans_Agy99 LVNVVTGGEAHEQR--AYGDFLDKQQRYRRTGEFLHVVRQLWA-------
.: :. * * : .*
MSMEG_6734|M.smegmatis_MC2_155 VVIDRERGVHTDFTKVHEINFHGKYYKCRGPLNTAPSPQG-RPTFLQAGG
Mflv_0250|M.gilvum_PYR-GCK IVYDRDNGVFADPAKVREVNFQGEFFQSRGRHFVAPSPQK-RPVLWQAGS
MAB_2482|M.abscessus_ATCC_1997 VVRDRGRGVFTDPAKVHDIEHHGQYFTVPGPFLCEPSAQR-TPLLFQAGA
TH_4268|M.thermoresistible__bu VIRDRERGVFTDPDKVHHIGHRGRHFSVPGVHLSEPSPQR-TPVIYQAGS
MAV_0340|M.avium_104 VLHDRVSGRYADPAKVHELGHVGEFFSARGPLTVPRTPQG-RPVIIQAGS
MMAR_0703|M.marinum_M VLADIENGDYVDPERIRSVEHSGPQFEVRGFATLPAGPQG-HPVLLQAGD
Mvan_6020|M.vanbaalenii_PYR-1 FVERKDTGQYLNARKVRVPDYKGRHLSVKGPLNVSRSPQG-RPVLFHAGQ
Mb0815c|M.bovis_AF2122/97 ---------------EPNPTYAGRFYTLDGATCDPPPVQRPHPPLWIGGE
Rv0791c|M.tuberculosis_H37Rv ---------------EPNPTYAGRFYTLDGATCDPPPVQRPHPPLWIGGE
MUL_4507|M.ulcerans_Agy99 --------------SGQPVTFEGEHIHVHQAQLGRLPDPP--PPVFFGGS
. * * . .*
MSMEG_6734|M.smegmatis_MC2_155 SPRGRDFAAKHSDAIITLADGVDGMKAYREDIRGRAAAQGRNPDEIKVMY
Mflv_0250|M.gilvum_PYR-GCK SAQGREFASRHAESVFGIFPTPKSMRAYATDIRTRASDAGRDPESVKLIY
MAB_2482|M.abscessus_ATCC_1997 SARGVRFAAAHAEAVFVSGPTPDVVRAPVRALRAEAARLGRDPRSVKVFT
TH_4268|M.thermoresistible__bu SPRGVRFAAENAEAIFTASPTKEILRRTVSTIRQELEIAGRDPYSVKIFN
MAV_0340|M.avium_104 SGRGREFASRWAELIFTGDPGIDVARSHYADQKDRIADAGRDPAAVRICP
MMAR_0703|M.marinum_M SEQGRAFGARHADAMFTLHSSLRDGQRYYADVKGRARSYGRDPDQLKVFP
Mvan_6020|M.vanbaalenii_PYR-1 SEGGRRLAARHADCIFEAAASVEAAREFYADLKRRRVEIGGEPDHLRIIV
Mb0815c|M.bovis_AF2122/97 GDRVQRIAAKHAQGLNVRWWSPQQVTQRRGFLTQASEAAGRDPDTLRLSV
Rv0791c|M.tuberculosis_H37Rv GDRVQRIAAKHAQGLNVRWWSPQQVTQRRGFLTQASEAAGRDPDTLRLSV
MUL_4507|M.ulcerans_Agy99 SESAGAVAARYADTYLTWGEPLSAVGKKIDWIRGLAADHGR---ALSYGL
. ..: :: * :
MSMEG_6734|M.smegmatis_MC2_155 LVIPTLGETTEEAVARRDRLTTSQAFAEQTLSLLSSITDIDFSQFDLDST
Mflv_0250|M.gilvum_PYR-GCK GLQTIIDRDKGRANDRYAEFVEN-VQIESALGILSGHTGFDFSTLGLDDN
MAB_2482|M.abscessus_ATCC_1997 MVTPIVAESHDAAVAKLLEYRRF-VSAAGALALFGGWTGVDLARLDPDEP
TH_4268|M.thermoresistible__bu LTTIITAETDEEAHARHAEYLAY-GDPEGALTFMSGWMGVDLARYGLDEP
MAV_0340|M.avium_104 MAYAVVGESEAHAKDREAMLLNDLVHPMASLTLLSELMNYDFAQHDLDDP
MMAR_0703|M.marinum_M AATFVIGDTPDQAGEKARQISYQQVSGATAIAMLEQVWGRDLSDYDPDGP
Mvan_6020|M.vanbaalenii_PYR-1 SVAVYLGRTESEAVELYSELNSL-ISPDLGVDFLAKAVSEDLTGYPVDGP
Mb0815c|M.bovis_AF2122/97 TLLLAPTQSGEEEVRIREEFASI--------------------------P
Rv0791c|M.tuberculosis_H37Rv TLLLAPTQSGEEEVRIREEFASI--------------------------P
MUL_4507|M.ulcerans_Agy99 RIHVITRDTAEEAWRVADRLLAG-IDPADIERMQANLARSESEGQRRMAE
MSMEG_6734|M.smegmatis_MC2_155 LPH---LTTNGEQGSLDKFQQ-SGSGK--TLRELIYDSAD------FCSS
Mflv_0250|M.gilvum_PYR-GCK VVD---ADVQGIRGLFDAILE-AKDGAPVTVREAAQIYGV------SMGA
MAB_2482|M.abscessus_ATCC_1997 LRH---VETEANRSALASFTN-DPK-RTWTTRELAQEIGI------GGRG
TH_4268|M.thermoresistible__bu VGN---VDSNAILSAVAAFQSADPDGREWAVRDIAEWGKI------GGMG
MAV_0340|M.avium_104 VTDDILASVSGIRGLVQNLRA-HIGGDAVTVRDLANHRAT------LLQG
MMAR_0703|M.marinum_M LPDIDPIADAAISQGRVRHADPVAVARRYRERSQAENLSIRELVIAVTSR
Mvan_6020|M.vanbaalenii_PYR-1 MPDLSAPVVGG--NSIRGQLDAIAKAEQLTVRQMYERVVP------TMGN
Mb0815c|M.bovis_AF2122/97 EPG-----------------------------------------------
Rv0791c|M.tuberculosis_H37Rv EPG-----------------------------------------------
MUL_4507|M.ulcerans_Agy99 LHG-------------GVLDQLEIAPNLWAGVGLVRGGAG----------
MSMEG_6734|M.smegmatis_MC2_155 YPLCGTPSSVADEMEWLMDEVGGDGFLITLHNQGVSRRHVIEVTDGLIPE
Mflv_0250|M.gilvum_PYR-GCK PVAVGTPADVADQMEQYLDEGDCNGFMMLATDT---PGCFNDMTELLVPE
MAB_2482|M.abscessus_ATCC_1997 PVLVGTGSSIADELERWSDEADIDGFNVAYVTT---PGTFADFARLVIPE
TH_4268|M.thermoresistible__bu PRIVGSGFTVADTLQEWVEETDVDGFNLAYAIT---PGSFAQFIEHVVPV
MAV_0340|M.avium_104 PRFVGTGPQVADQMADWFQTGACDGFVLAATHL---PGAFEDVVRMVVPE
MMAR_0703|M.marinum_M QQFVGTPSHIADEIDRYVQADACDGFILVPHLT---PHGLDEFVDRVVPL
Mvan_6020|M.vanbaalenii_PYR-1 TALIGTATQIADVMEEWYTTGACDGFVLGASIS---PFTLLLIRDELVPE
Mb0815c|M.bovis_AF2122/97 -LIVGTPDRCVERIREYQDRGVGHFLFTIPHVVK--SDYLHIIGSDIIPR
Rv0791c|M.tuberculosis_H37Rv -LIVGTPDRCVERIREYQDRGVGHFLFTIPHVVK--SDYLHIIGSDIIPR
MUL_4507|M.ulcerans_Agy99 TALVGSHQEVAERIIEYARLGISHFILSGYPHL----EEAYWFGEGVLPI
*: .: : . : . ::*
MSMEG_6734|M.smegmatis_MC2_155 LQGRGVVRTEYTQNTLRETLREF---------------------------
Mflv_0250|M.gilvum_PYR-GCK LQKRGRFRTRYPGTTLRESLREY---------------------------
MAB_2482|M.abscessus_ATCC_1997 LRRRGRVPHQRERCTLREQLGGSGPYLADTHPGTGYRR------------
TH_4268|M.thermoresistible__bu LTERGAYQPAYTPGTLRHKLFGKGDRLPDDHRGSRYRVGGPLSTIIDRPS
MAV_0340|M.avium_104 LQRRGLFRTEYRSATLRGHLGLPRPANARVAHA-----------------
MMAR_0703|M.marinum_M LQERGAFRTAYTGQTLRDHLGLAGLPS-----------------------
Mvan_6020|M.vanbaalenii_PYR-1 LQRRGLFRRHYTGSTLRENLGLPPVDNSPFAT------------------
Mb0815c|M.bovis_AF2122/97 VKTEVTIP------------------------------------------
Rv0791c|M.tuberculosis_H37Rv VKTEVTIP------------------------------------------
MUL_4507|M.ulcerans_Agy99 LERKRLWHRPHGSSPHSAALPVAHSSVN----------------------
: .
MSMEG_6734|M.smegmatis_MC2_155 -----------------
Mflv_0250|M.gilvum_PYR-GCK -----------------
MAB_2482|M.abscessus_ATCC_1997 -----------APLPG-
TH_4268|M.thermoresistible__bu TLPSSPASAASQPISGR
MAV_0340|M.avium_104 -----------------
MMAR_0703|M.marinum_M -----------------
Mvan_6020|M.vanbaalenii_PYR-1 -----------------
Mb0815c|M.bovis_AF2122/97 -----------------
Rv0791c|M.tuberculosis_H37Rv -----------------
MUL_4507|M.ulcerans_Agy99 -----------------