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MTAQPRQLHLNAFLMGVGHHEAAWRHPRTDARNLTDVKHFQRLAQLAERGRLDSVFFADTLAVGPRVKHN SQTIFEPITLLTAMAAVTEHIGLIATASTGYNAPYTLARAFASLDHISGGRAGWNIVTSAGEDEAANFGY HGIPDHTGRYARAAEFLDVTTALWDSWEDDALVLDEHSGIFADPTKVHRIDHDGERFRIRGPLNSPRSPQ GHPLLVQAGSSETGKDFAARYAEAIFTAQRSLDEGKAFYRDVKARAVRFGRSPDDVKILPGVVVYLGETA RAAQELEQEFTDLISPEYSLRQLSQMLGVDLTAHSLDAPLPPLPPIEQIQGNKSRYQLVKDLAAGESLTV RQLIAKLGGGRGHRTFAGTPEQVADNLEEWFTEGAADGFNVMPPYLPGGLEDFIEHVVPILQRRGLFRTE YTATTLRGHYGLAYQPSRHSRTAAESTA
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. smegmatis MC2 155 | MSMEG_1162 | - | - | 100% (448) | nitrilotriacetate monooxygenase component A |
| M. smegmatis MC2 155 | MSMEG_4590 | ssuD | e-103 | 45.66% (449) | nitrilotriacetate monooxygenase component A |
| M. smegmatis MC2 155 | MSMEG_2842 | ssuD | 2e-99 | 44.96% (427) | nitrilotriacetate monooxygenase component A |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb0978c | - | 2e-08 | 29.63% (189) | oxidoreductase |
| M. gilvum PYR-GCK | Mflv_0250 | - | 3e-69 | 35.25% (434) | hypothetical protein Mflv_0250 |
| M. tuberculosis H37Rv | Rv0953c | - | 2e-08 | 29.63% (189) | oxidoreductase |
| M. leprae Br4923 | MLBr_00269 | - | 4e-06 | 22.76% (268) | putative F420-dependent glucose-6-phosphate dehydrogenase |
| M. abscessus ATCC 19977 | MAB_4125 | - | 6e-86 | 42.46% (431) | putative nitrilotriacetate monooxygenase |
| M. marinum M | MMAR_0703 | - | 2e-86 | 39.01% (446) | FMNH2-utilizing oxygenase |
| M. avium 104 | MAV_0340 | - | 1e-91 | 43.03% (423) | nitrilotriacetate monooxygenase component A |
| M. thermoresistible (build 8) | TH_4079 | - | 1e-127 | 55.50% (409) | PUTATIVE nitrilotriacetate monooxygenase component A |
| M. ulcerans Agy99 | MUL_0292 | - | 7e-09 | 28.72% (195) | hypothetical protein MUL_0292 |
| M. vanbaalenii PYR-1 | Mvan_6020 | - | 6e-91 | 41.61% (435) | xenobiotic compound DszA family monooxygenase |
CLUSTAL 2.0.9 multiple sequence alignment
MSMEG_1162|M.smegmatis_MC2_155 -MTAQPRQLHLNAFLMG--VGHHEAAWRHPRTD-ARNLTDVKHFQRLAQL
TH_4079|M.thermoresistible__bu ----------------------------LPETR-LSGITDIDHYLELARI
Mvan_6020|M.vanbaalenii_PYR-1 -------MMHLITWLMG--NSYHAAGWRHQLAW-ERTAMRLDVLIEIAKI
MAB_4125|M.abscessus_ATCC_1997 --MSSRNGRQLHLLAFG--NVRAGGAWRLPGVR-NGPANQLNTLVATAKA
Mflv_0250|M.gilvum_PYR-GCK MTDSAKRKAHLLGFVQHGVMNHASTMWAHPRDKVGYHWSRPEYWRDLGRT
MAV_0340|M.avium_104 ---MRTDKMALVAFMQAGSTSVYAGSWRHPAN--EHRYLDAAYYAKIGRQ
MMAR_0703|M.marinum_M ---MIRKPVHLAAHFPG---VNNTTVWADPGAG---SQIEFESFVHLART
Mb0978c|M.bovis_AF2122/97 ------MHYGLVLFTSDRGITPAAAARLAESHGFRTFYVPEHTHIPVKRQ
Rv0953c|M.tuberculosis_H37Rv ------MHYGLVLFTSDRGITPAAAARLAESHGFRTFYVPEHTHIPVKRQ
MUL_0292|M.ulcerans_Agy99 ----------------------------MDLVG-IAPEQHWAVMRDLATY
MLBr_00269|M.leprae_Br4923 ------------------------MAELRLGYKASAEQFAPRELVELGVA
MSMEG_1162|M.smegmatis_MC2_155 AERGRLDSVFFADTLAVG---------PRVKHNSQTIFEPITLLTAMAAV
TH_4079|M.thermoresistible__bu AERGKLDAIFFADHPVLK---------GKTEFRPFDALDPVTLVTALSTA
Mvan_6020|M.vanbaalenii_PYR-1 AEEAKLDALFVADGNGVRNMDKVGLFEANTPSARPTVYDPVTLMAAISQH
MAB_4125|M.abscessus_ATCC_1997 AEAAKFDAVFFADGLNFG-------PKATWAHKSTEDFEPLTATSYLAAV
Mflv_0250|M.gilvum_PYR-GCK MERGLFDAMFIADELAPYTTYKGSSDPVVKFAGQCPVHEPASVVPIVGAF
MAV_0340|M.avium_104 LEEGCFDLMFFDDRLAMPGVYGGSVGEAVRYGARPVKLDLGVILGVLAQA
MMAR_0703|M.marinum_M AERGLFDFFFLAEGLRLREHRG-----RIHDLDVVGRPDTFTLLAALAGV
Mb0978c|M.bovis_AF2122/97 AAHPTTGDASLPDDRYMR------------------TLDPWVSLGAASAV
Rv0953c|M.tuberculosis_H37Rv AAHPTTGDASLPDDRYMR------------------TLDPWVSLGAASAV
MUL_0292|M.ulcerans_Agy99 ADASAWDSLWVYDHFHTVP-----------LPSDEATHEAWSLMAAYAAV
MLBr_00269|M.leprae_Br4923 AEAHGMDSATVSDHFQPWR------------HQGGHASFSLSWMTAVGER
. . : .
MSMEG_1162|M.smegmatis_MC2_155 TEHIGLIATAST--GYNAPYTLARAFASLDHISGGRAGWNIVTSAGEDEA
TH_4079|M.thermoresistible__bu TTHIGLVATAST--TYTEPYGLARRFATLDHVSRGRAGWNIVTTANAAAA
Mvan_6020|M.vanbaalenii_PYR-1 TKHIGLVGTAST--TYESPWVVARRFASLDHLSNGRASWNVVTGSNPGDS
MAB_4125|M.abscessus_ATCC_1997 TERLGLVVTGSS--TFQPPYHLARQLLSLDHLSSGRAGWNLVTSFAQAAA
Mflv_0250|M.gilvum_PYR-GCK TKHLGIGITLST--SFVPPYMMARQLSTLDHLTGGRVGWNIVTSYSKSEF
MAV_0340|M.avium_104 TSRIGLGATYST--TYYPPFHVARTFASLDHLSGGRAAWNVVTSVNDSEA
MMAR_0703|M.marinum_M TDRIGLTGTINT--TFNEPFEVARQFATLDHLSEGRAGWNIVTSSDAFTG
Mb0978c|M.bovis_AF2122/97 TSRIRLATAVALP-VEHDPITLAKSIATLDHLSHGRVSVGVGFGWNTDEL
Rv0953c|M.tuberculosis_H37Rv TSRIRLATAVALP-VEHDPITLAKSIATLDHLSHGRVSVGVGFGWNTDEL
MUL_0292|M.ulcerans_Agy99 TSRIKLGQMCTAM-SYRNPVYLAKVAATADIISGGRIQMGIGGGWYEHEW
MLBr_00269|M.leprae_Br4923 TNRILLGTSVLTPTFRYNPAVIGQAFATMGCLYPNRVFLGVGTGEALNEV
* :: : * :.: : . : .* .:
MSMEG_1162|M.smegmatis_MC2_155 ANFGYHGIPDHTGRYARAAEFLDVTTALWDSWEDDALVLDEHSGIFADPT
TH_4079|M.thermoresistible__bu ANFGRPAHPDPEHRYSRADEFLQVALRLWDSWEDDAIVGDKQAPRFVDPD
Mvan_6020|M.vanbaalenii_PYR-1 ENFGLAHHPDRDSRYARAEEFVSACKALWNSWDEDAFVERKDTGQYLNAR
MAB_4125|M.abscessus_ATCC_1997 ANFGERGFLPHDERYRLAEETLQVVKSLWHSLEPDTVIEDRAAGIYSDIN
Mflv_0250|M.gilvum_PYR-GCK QAMGKENLTPRDKRYEVVEEYMQLLYQLWDSWDDDAIVYDRDNGVFADPA
MAV_0340|M.avium_104 QNFGVDAHLGHDERYDRADEFMDVVTGLWDTWEDDAVLHDRVSGRYADPA
MMAR_0703|M.marinum_M ANFRRGGYLDHADRYQRAEEFLTVARKFWDSWAPGAVLADIENGDYVDPE
Mb0978c|M.bovis_AF2122/97 VDHG----VPPGRRRTMLREYLEAMRALWT--------------------
Rv0953c|M.tuberculosis_H37Rv VDHG----VPPGRRRTMLREYLEAMRALWT--------------------
MUL_0292|M.ulcerans_Agy99 RAYG-YGFPSAGVRLGRLDEGVQIMRDAWRD-------------------
MLBr_00269|M.leprae_Br4923 ATGYQGAWPEFKERFARLRESVRLMRELWRG-------------------
* * : *
MSMEG_1162|M.smegmatis_MC2_155 KVHRIDHDGERFRIRGPLNSPRSPQ--GHPLLVQAGSSETGKDFAARYAE
TH_4079|M.thermoresistible__bu KIHAIDHRGEHFSVAGPLEVPRSPQ--GHPVLFQAGSSEPGKQLAAKYAE
Mvan_6020|M.vanbaalenii_PYR-1 KVRVPDYKGRHLSVKGPLNVSRSPQ--GRPVLFHAGQSEGGRRLAARHAD
MAB_4125|M.abscessus_ATCC_1997 KIHVANHDGEFHKVKGPLGVTPSRQ--GHPVIFQAGSSTTGRAFAARHAE
Mflv_0250|M.gilvum_PYR-GCK KVREVNFQGEFFQSRGRHFVAPSPQ--KRPVLWQAGSSAQGREFASRHAE
MAV_0340|M.avium_104 KVHELGHVGEFFSARGPLTVPRTPQ--GRPVIIQAGSSGRGREFASRWAE
MMAR_0703|M.marinum_M RIRSVEHSGPQFEVRGFATLPAGPQ--GHPVLLQAGDSEQGRAFGARHAD
Mb0978c|M.bovis_AF2122/97 -QEEACYDGEFVKFGPSWAWPKPVQP-HIPVLVGAAGTEKNFKWIARSAD
Rv0953c|M.tuberculosis_H37Rv -QEEACYDGEFVKFGPSWAWPKPVQP-HIPVLVGAAGTEKNFKWIARSAD
MUL_0292|M.ulcerans_Agy99 --GKVSFDGKHYQVDGAIVAPKPLQNNGIPLWVAGGGEKVTLRIAAQYAQ
MLBr_00269|M.leprae_Br4923 --DRVDFDGDYYQLKGASIYDVPEG--GVPIYIAAGGPEVAKYAGRAGEG
. * *: ..
MSMEG_1162|M.smegmatis_MC2_155 AIFTAQRSLDEGKAFYR-DVKARAVRFGRSPDDVKILPGVVVYLGETARA
TH_4079|M.thermoresistible__bu AIFTAQPTVEEARQFYA-DVKARVRQHGRDPDRVHILPGLSAIIGGTEEE
Mvan_6020|M.vanbaalenii_PYR-1 CIFEAAASVEAAREFYA-DLKRRRVEIGGEPDHLRIIVSVAVYLGRTESE
MAB_4125|M.abscessus_ATCC_1997 VVFTGQTDVERAKNFYR-QLHQEARAAGRTFAPL-VTPSLGVVVGSTEAE
Mflv_0250|M.gilvum_PYR-GCK SVFGIFPTPKSMRAYAT-DIRTRASDAGRDPESVKLIYGLQTIIDRDKGR
MAV_0340|M.avium_104 LIFTGDPGIDVARSHYA-DQKDRIADAGRDPAAVRICPMAYAVVGESEAH
MMAR_0703|M.marinum_M AMFTLHSSLRDGQRYYA-DVKGRARSYGRDPDQLKVFPAATFVIGDTPDQ
Mb0978c|M.bovis_AF2122/97 GWITTPRDVDID-------------------EPVKLLQDIWAAAGRDG--
Rv0953c|M.tuberculosis_H37Rv GWITTPRDVDID-------------------EPVKLLQDIWAAAGRDG--
MUL_0292|M.ulcerans_Agy99 YTNFTPQ-PEQFAHKSQ-VLAEHCRKLGTEFDAIVRSANFTAVLGTAEAD
MLBr_00269|M.leprae_Br4923 FVCTSGKGEELYTEKLIPAVLEGAAVAGRDADDIDKMIEIKMSYDPDPEQ
: .
MSMEG_1162|M.smegmatis_MC2_155 AQELEQEFTDL-ISPEYSLRQLSQMLGVDLTAHSLDAPLPPLPPIEQIQG
TH_4079|M.thermoresistible__bu ARRIQRRLEDL-TVPDYGLEQLSRIVGYRVGRDDLDRRLQFGSDDPPAIH
Mvan_6020|M.vanbaalenii_PYR-1 AVELYSELNSL-ISPDLGVDFLAKAVSEDLTGYPVDGPMPDLS--APVVG
MAB_4125|M.abscessus_ATCC_1997 ALAAESTIYEH-FIPEYQAGWLLEVD-VNVVGADLDGPVPASAFPDSTET
Mflv_0250|M.gilvum_PYR-GCK ANDRYAEFVEN-VQIESALGILSGHTGFDFSTLGLDDNVVD----ADVQG
MAV_0340|M.avium_104 AKDREAMLLNDLVHPMASLTLLSELMNYDFAQHDLDDPVTDDIL-ASVSG
MMAR_0703|M.marinum_M AGEKARQISYQQVSGATAIAMLEQVWGRDLSDYDPDGPLPDIDPIADAAI
Mb0978c|M.bovis_AF2122/97 ---------------------LPQIVALDVKP------------------
Rv0953c|M.tuberculosis_H37Rv ---------------------LPQIVALDVKP------------------
MUL_0292|M.ulcerans_Agy99 VKDRLGRVRDR------LVGYVPEAVADSMVGGLPDS-------------
MLBr_00269|M.leprae_Br4923 ALSNIRFWAPLSLAAEQKHSIDDPIEMEKVADALPIEQVAKR--------
.
MSMEG_1162|M.smegmatis_MC2_155 NKSRYQLVKDLAAGESLTVRQLIAKLGG------GRGHRTFAGTPEQVAD
TH_4079|M.thermoresistible__bu MQSRYALVRRLVSTENLTVRELLLRLSG------GRGHRVFAGTPEQVAD
Mvan_6020|M.vanbaalenii_PYR-1 GNSIRGQLDAIAKAEQLTVRQMYERVVP------TMGNTALIGTATQIAD
MAB_4125|M.abscessus_ATCC_1997 HQTALAGYKHLATVGNPTVREFLYRTLN------AFGTR-FVGSAEQVAD
Mflv_0250|M.gilvum_PYR-GCK IRGLFDAILEAKDGAPVTVREAAQIYGV------SMGAPVAVGTPADVAD
MAV_0340|M.avium_104 IRGLVQNLRAHIGGDAVTVRDLANHRAT------LLQGPRFVGTGPQVAD
MMAR_0703|M.marinum_M SQGRVRHADPVAVARRYRERSQAENLSIRELVIAVTSRQQFVGTPSHIAD
Mb0978c|M.bovis_AF2122/97 -----------------------------------------------VPD
Rv0953c|M.tuberculosis_H37Rv -----------------------------------------------VPD
MUL_0292|M.ulcerans_Agy99 ----------------------------------------ATGTPEQVIE
MLBr_00269|M.leprae_Br4923 --------------------------------------WIVVSDPDEAVA
MSMEG_1162|M.smegmatis_MC2_155 NLEEWFTEGAADGFNVMPPYLPG--GLEDFIEHVVPILQRRGLFRTEYTA
TH_4079|M.thermoresistible__bu TIEEWFTTGAADGFNLIPPALPS--SLTAFVDHVVPELQRRGLFRTEYTG
Mvan_6020|M.vanbaalenii_PYR-1 VMEEWYTTGACDGFVLGASISPF--TLLLIRDELVPELQRRGLFRRHYTG
MAB_4125|M.abscessus_ATCC_1997 QIELWFSEGAADGFILSTSGLPG--QFELFTEHVVPILVRRGLFRAEYTG
Mflv_0250|M.gilvum_PYR-GCK QMEQYLDEGDCNGFMMLATDTPG--CFNDMTELLVPELQKRGRFRTRYPG
MAV_0340|M.avium_104 QMADWFQTGACDGFVLAATHLPG--AFEDVVRMVVPELQRRGLFRTEYRS
MMAR_0703|M.marinum_M EIDRYVQADACDGFILVPHLTPH--GLDEFVDRVVPLLQERGAFRTAYTG
Mb0978c|M.bovis_AF2122/97 KLARWAELGVTEVLFGMPDRSAD--DAAAYVERLAAKLACCV--------
Rv0953c|M.tuberculosis_H37Rv KLARWAELGVTEVLFGMPDRSAD--DAAAYVERLAAKLACCV--------
MUL_0292|M.ulcerans_Agy99 RLAQIRDLGCEYAICYFPEAAYDRSGIELFEREVIPALS-----------
MLBr_00269|M.leprae_Br4923 RVGQYVTWGLNHLVFHAPGHNQRR-FLELFEKDLAPRLRRLG--------
: . . . : . *
MSMEG_1162|M.smegmatis_MC2_155 TTLRGHYGLAYQPSRHSRTAAESTA-
TH_4079|M.thermoresistible__bu TTLRDHLGLPRPRNRFTTGRPVAAAS
Mvan_6020|M.vanbaalenii_PYR-1 STLRENLGLPPVDN-----SPFAT--
MAB_4125|M.abscessus_ATCC_1997 STLREHLALPWPVP----ARPPALLA
Mflv_0250|M.gilvum_PYR-GCK TTLRESLREY----------------
MAV_0340|M.avium_104 ATLRGHLGLPRPANARVAHA------
MMAR_0703|M.marinum_M QTLRDHLGLAGLPS------------
Mb0978c|M.bovis_AF2122/97 --------------------------
Rv0953c|M.tuberculosis_H37Rv --------------------------
MUL_0292|M.ulcerans_Agy99 --------------------------
MLBr_00269|M.leprae_Br4923 --------------------------