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M. smegmatis MC2 155 MSMEG_0578 (-)

annotation: acyl-CoA dehydrogenase middle domain-containing protein
coordinates: 654135 - 655271
length: 378

MDFSRVELSDEDTRFRDELRAFLAEIVTDEVIRRDRETGENFDESVHLALGGAGYLAADYRCADDGGFSA
VRRRIWELEIGRAHTPWFHWGTTAMAAHTVEKFGTPELVEEVLPGVLSGRVRICLGYTEPEGGSDVATCK
TRAVRDGDAWIINGSKMFTSNAHNAQYVFLVTNTDPEAPKHNSLTMFLVPLGTPGVEIQGIRTVDGDRTN
ITYYSDVRVSDRYRVGDVNGGWGVLRGALDVEHGTFERDTHGLQKIATMTEHITLMAEALDLVAAQAPGD
GASRFRLGRGIACTEAAMSTPDMYGRVAIAQTMRDVTPDLMDILGPAGALTDGVEYIFRLAGPTGIYGGT
LEVFRNMIAQHGLGLGKPNYAQPVKKVS

Operon Prediction Model: Genebank

Paralogs

species id gene e-value identity (len) annotation
M. smegmatis MC2 155 MSMEG_0578 - - 100% (378) acyl-CoA dehydrogenase middle domain-containing protein
M. smegmatis MC2 155 MSMEG_2234 - e-146 64.65% (396) acyl-CoA dehydrogenase middle domain-containing protein
M. smegmatis MC2 155 MSMEG_5993 - 1e-36 35.00% (240) putative acyl-CoA dehydrogenase

Closest Orthologs (e-value cutoff: 1e-4)

species id gene e-value identity (len) annotation
M. bovis AF2122 / 97 Mb3573c fadE29 6e-33 33.33% (240) acyl-CoA dehydrogenase FADE29
M. gilvum PYR-GCK Mflv_4100 - 1e-161 72.89% (380) acyl-CoA dehydrogenase domain-containing protein
M. tuberculosis H37Rv Rv3543c fadE29 6e-33 33.33% (240) acyl-CoA dehydrogenase FADE29
M. leprae Br4923 MLBr_00737 fadE25 1e-15 23.99% (396) putative acyl-CoA dehydrogenase
M. abscessus ATCC 19977 MAB_0615 - 5e-41 38.02% (242) putative acyl-CoA dehydrogenase
M. marinum M MMAR_4010 - 1e-143 66.07% (389) acyl-CoA dehydrogenase
M. avium 104 MAV_5127 - 1e-150 67.52% (391) acyl-CoA dehydrogenase middle domain-containing protein
M. thermoresistible (build 8) TH_2587 - 1e-159 70.78% (397) PUTATIVE acyl-CoA dehydrogenase domain protein
M. ulcerans Agy99 MUL_3874 - 1e-142 65.81% (389) acyl-CoA dehydrogenase
M. vanbaalenii PYR-1 Mvan_2246 - 1e-158 69.97% (383) acyl-CoA dehydrogenase domain-containing protein

CLUSTAL 2.0.9 multiple sequence alignment


Mb3573c|M.bovis_AF2122/97           -------------MFIDLTPEQRQLQAEIRQYFSNLISPDERTEMEKDRH
Rv3543c|M.tuberculosis_H37Rv        -------------MFIDLTPEQRQLQAEIRQYFSNLISPDERTEMEKDRH
MAB_0615|M.abscessus_ATCC_1997      -------------MLIDLTPEQAKLQNDLRHYFSNLVTAEETREMMIDRH
Mflv_4100|M.gilvum_PYR-GCK          MG------VAVDFSEVELCDADKAFRDEVREFLAAVVT-DDVIRRDLESG
Mvan_2246|M.vanbaalenii_PYR-1       MHGQEVGAVTMDFSEVELSDDDRAFQAELRGFLARVVT-DDVIERDRRTG
MSMEG_0578|M.smegmatis_MC2_155      ----------MDFSRVELSDEDTRFRDELRAFLAEIVT-DEVIRRDRETG
TH_2587|M.thermoresistible__bu      ----------MDFSTVELSPEDQRFRAELRDFLTRHVT-AEVIRRDRETG
MAV_5127|M.avium_104                ----------MDFSRVELSTQDAAFQESTRAFLAEHVT-DEVRRRDRETG
MMAR_4010|M.marinum_M               ----------MDFSRVQISDADREFRETARSFLATHVT-EEVRRHDRETG
MUL_3874|M.ulcerans_Agy99           ----------MDFSRVQISDADREFRETARSFLATHVT-EEVRRHDRETG
MLBr_00737|M.leprae_Br4923          ---MVGWSGNPLFDLFKLPEEHNELRATIRALAEKEIA---PHAADVDQR
                                                   ..:   .  ::   *      ::            

Mb3573c|M.bovis_AF2122/97           GPAYRAVIRRMGRDGRLGVGWPKEFGGLGFGPIEQQIFVNEAHRADVP-L
Rv3543c|M.tuberculosis_H37Rv        GPAYRAVIRRMGRDGRLGVGWPKEFGGLGFGPIEQQIFVNEAHRADVP-L
MAB_0615|M.abscessus_ATCC_1997      GSSYEAVVRRMGQDGWLGVGWPKEYGGHGFGPLEQQIFMNEVMRADVP-M
Mflv_4100|M.gilvum_PYR-GCK          DNFDEQVHLALGSAGYLERDWRAEADG-GFTAVQRRIFELEIGRAHTPWF
Mvan_2246|M.vanbaalenii_PYR-1       DNFDETVHLALGAAGYLERDWRAGQDG-GFTAVQRRIFELEIGRAHTPWF
MSMEG_0578|M.smegmatis_MC2_155      ENFDESVHLALGGAGYLAADYRCADDG-GFSAVRRRIWELEIGRAHTPWF
TH_2587|M.thermoresistible__bu      DNFDEGVHTALGAAGYLEKDFRSEAEG-GWNALRRRIWELEIGRAHTPWF
MAV_5127|M.avium_104                ENFSEPVHVALGEAGYLESDWKLESEG-GFDPVRRRIFQLEIGRAHTPWF
MMAR_4010|M.marinum_M               DNFHEGLHLALGAAGYLAAEWKPEADG-GFSRVRRRIWELEKRRAHVPWV
MUL_3874|M.ulcerans_Agy99           DNFHEGLHLALGAAGYLAAEWKPEADG-GFSRVRRRIWELEKRRAHVPWV
MLBr_00737|M.leprae_Br4923          ARFPEEALAALNASGFNAIHVPEEYGGQGADSVAACIVIEEVARVDASAS
                                        .     :.  *           * *   :   *   *  *....  

Mb3573c|M.bovis_AF2122/97           PAVTLQTVGPT-LQAHGSELQKKKFLPAILAGEAHFAIGYTEPEAGTDLA
Rv3543c|M.tuberculosis_H37Rv        PAVTLQTVGPT-LQAHGSELQKKKFLPAILAGEAHFAIGYTEPEAGTDLA
MAB_0615|M.abscessus_ATCC_1997      PLVTLQTVGPT-LQKYGTEEQKKKFLPGILAGEIHFAIGYTEPEAGTDLA
Mflv_4100|M.gilvum_PYR-GCK          HWPTTAMVANA-VDHFGSDELKDEVLPQVLAGRYRLCLGYTEPEGGSDIA
Mvan_2246|M.vanbaalenii_PYR-1       HWGTTSMVARA-LDQFGSDELKDEVLDRVLSGEFRLCLGYTEPDGGSDVA
MSMEG_0578|M.smegmatis_MC2_155      HWGTTAMAAHT-VEKFGTPELVEEVLPGVLSGRVRICLGYTEPEGGSDVA
TH_2587|M.thermoresistible__bu      HWGTTAMVAQS-VEKFGTPELRDEVIPGVLTGEIRLCLGYTEPEGGSDVA
MAV_5127|M.avium_104                HWGTTAVVARL-VKQFGAPELADAILPGVLSGKIRLCLGYTEPEGGSDVA
MMAR_4010|M.marinum_M               TWGTTAMVARS-VAKFGSPELVEEVLPGVFSGHVRMCLGYTEPEGGSDVA
MUL_3874|M.ulcerans_Agy99           TWGTTAMVARS-VAKFGSPELVEEVLPGVFSGHVRMCLGYTEPEGGSDVA
MLBr_00737|M.leprae_Br4923          LIPAVNKLGTMGLILRGSEELKKQVLPSLAAEGAMASYALSEREAGSDAA
                                       :    .   :   *:    . .:  : :     . . :* :.*:* *

Mb3573c|M.bovis_AF2122/97           SLRTTAVRDGDH--YIVNGQKVFTTGAHDADYIWLACRTDPNAAKHKGIS
Rv3543c|M.tuberculosis_H37Rv        SLRTTAVRDGDH--YIVNGQKVFTTGAHDADYIWLACRTDPNAAKHKGIS
MAB_0615|M.abscessus_ATCC_1997      SLRTTAVRDGDH--YVVNGQKIFTTGAHQAQYIWLACRTDPSAPKHKGIS
Mflv_4100|M.gilvum_PYR-GCK          TCKTRAVREADGSTWIINGSKMFTSNAHHAQYVYLITNTDPQAPKHQSLT
Mvan_2246|M.vanbaalenii_PYR-1       TCKTRAVRDGDG--WIVNGSKMFTSNAHHAQYVFLITNTDPAAPKHQSLT
MSMEG_0578|M.smegmatis_MC2_155      TCKTRAVRDGDA--WIINGSKMFTSNAHNAQYVFLVTNTDPEAPKHNSLT
TH_2587|M.thermoresistible__bu      TCRTRAVREADGSSWIINGAKMFTSNAHHAQYVFLLTNTDPAAPKHQSLT
MAV_5127|M.avium_104                TCKTRAVRDGSG--WIINGSKMFTSNAQNAEYVFLLTNTDPRRPKHKNLT
MMAR_4010|M.marinum_M               TCKTRALRDGDS--WLINGSKMFTTGAHNCQYVFLITNTDPQARKHKSLT
MUL_3874|M.ulcerans_Agy99           TCKTRALRDGDS--WLINGSKMFTTGAHNCQYVFLITNTDPQARKHKSLT
MLBr_00737|M.leprae_Br4923          SMRTRAKADGDD--WILNGFKCWITNGGKSTWYTVMAVTDPDKG-ANGIS
                                    : :* *  :..   :::** * : :.. .. :  :   ***     :.::

Mb3573c|M.bovis_AF2122/97           ILIVDTKDPGYSWTP--IILADGAHHTNATYYNDVRVPVDMLVGKENDGW
Rv3543c|M.tuberculosis_H37Rv        ILIVDTKDPGYSWTP--IILADGAHHTNATYYNDVRVPVDMLVGKENDGW
MAB_0615|M.abscessus_ATCC_1997      ILIVDTKDPGYSWTP--IITNDGAHHTNATYYTDVRVPVEMLVGEENAGW
Mflv_4100|M.gilvum_PYR-GCK          MFLVPLDSPGVEIQP--IRTVD-GDRTNITFYSDVRVDDRYRIGPVNGGW
Mvan_2246|M.vanbaalenii_PYR-1       MFLVPLDATGVEIQP--IRTVD-GDRTNITFYSDVRIDDRYRVGAVNGGW
MSMEG_0578|M.smegmatis_MC2_155      MFLVPLGTPGVEIQG--IRTVD-GDRTNITYYSDVRVSDRYRVGDVNGGW
TH_2587|M.thermoresistible__bu      MFLVPLDTPGIEIGP--IRTVD-GDRTNIVYYSDVRVDDHYRIGEVNGGW
MAV_5127|M.avium_104                MFLVPLDSPGIEIQG--IRTVD-GDRTNIVYYSDVRVDDLYRIGEVNGGW
MMAR_4010|M.marinum_M               MFLVPLDSAGIEIQG--IRTVD-GDRTNIVYYSDVRIDDKYRLGAVNGGW
MUL_3874|M.ulcerans_Agy99           MFLVPLDSAGIEIQG--IRTVD-GDRTNIVYYSDVRIDDKYRLGAVNGGW
MLBr_00737|M.leprae_Br4923          AFIVHKDDEGFSIGPKEKKLGIKGSPTTELYFDKCRIPGDRIIGEPGTGF
                                     ::*     * .           .  *.  :: . *:     :*  . *:

Mb3573c|M.bovis_AF2122/97           RLITTQLNNERVMLGPAGR-----FASIYDRVHAWASVPGGNGVTPIDHD
Rv3543c|M.tuberculosis_H37Rv        RLITTQLNNERVMLGPAGR-----FASIYDRVHAWASVPGGNGVTPIDHD
MAB_0615|M.abscessus_ATCC_1997      KLITTQLNHERVSLGPSGR-----IAGMYDRVYEWASKPGPDGVAPLSHE
Mflv_4100|M.gilvum_PYR-GCK          GVLREALNAEHGTVERDAS-----GLQKIAVMTEHALLLAEEVDRVASVV
Mvan_2246|M.vanbaalenii_PYR-1       AVLREALNAEHGTVEHDDS-----GLNKIAVMTEHATLLADEVDRIAGEM
MSMEG_0578|M.smegmatis_MC2_155      GVLRGALDVEHGTFERDTH-----GLQKIATMTEHITLMAEALDLVAAQA
TH_2587|M.thermoresistible__bu      TVLREALNVEHGTVERDSQ-----GLQKLATMTEHALLTAEGLDWAVAVA
MAV_5127|M.avium_104                TVMRFALDAEHGITDPQDH-----GLQNISMLSEHGHLMAEAADGVAAIL
MMAR_4010|M.marinum_M               TVLREPLNVEHGAVAAAPD-----GLQDTSIMMHQAGSMAEAVDRAASCV
MUL_3874|M.ulcerans_Agy99           TVLREPLNVEHGAVAAAPD-----GLQDTSIMMHQAGSMAEAVDRAASCV
MLBr_00737|M.leprae_Br4923          KTALATLDHTRPTIGAQAVGIAQGALDAAIVYTKDRKQFGESISTFQSIQ
                                          *:  :                            .          

Mb3573c|M.bovis_AF2122/97           DVKRALG-EIRAIWRINELLNWQVASAGEDINMADAAATKVFGTERVQRA
Rv3543c|M.tuberculosis_H37Rv        DVKRALG-EIRAIWRINELLNWQVASAGEDINMADAAATKVFGTERVQRA
MAB_0615|M.abscessus_ATCC_1997      DVRRVLG-EMKAVWRLNELLNWQVASSGDDISMADAAATKILATEWIQKL
Mflv_4100|M.gilvum_PYR-GCK          G-------DRLGDGEVAYRLGRSVARLEAALSTPEMFGRVAIAQTLRDIT
Mvan_2246|M.vanbaalenii_PYR-1       YRS-----GRLDDDSIGYRLGRSIARLEAALSTPEMFGRVAIAQTLRDIT
MSMEG_0578|M.smegmatis_MC2_155      P----------GDGASRFRLGRGIACTEAAMSTPDMYGRVAIAQTMRDVT
TH_2587|M.thermoresistible__bu      ASPGPDGRRLLDDESVQYRLGRSVARLEAALSTPAEYGRVAIAQMMRDIT
MAV_5127|M.avium_104                ARPDAAGRRPIDDESVKYRLGRGMARIEAAMSTPGMYGRVALIQTMREVS
MMAR_4010|M.marinum_M               MRPDPDGRRPIDDTSVAYRLGRSVARMEAALSAPSIFGRVAIAQTMRDIS
MUL_3874|M.ulcerans_Agy99           MRPDPDGRRPIDDTSVAYRLGRSVARMEAALSAPSIFGRVAIAQTMRDIS
MLBr_00737|M.leprae_Br4923          FMLADMAMKVEAARLIVYAAAARAERGEPDLGFISAASKCFASDIAMEVT
                                                                  :.     .         :  

Mb3573c|M.bovis_AF2122/97           GRLAEEIVGKYGNPAEPDTAELLRWLDAQTKRNLVITFGGGVNEVMREMI
Rv3543c|M.tuberculosis_H37Rv        GRLAEEIVGKYGNPAEPDTAELLRWLDAQTKRNLVITFGGGVNEVMREMI
MAB_0615|M.abscessus_ATCC_1997      GRLSEGVVGRYGDPADANTAETLEWLDAQTKRHLVITFGGGVNEVMREMV
Mflv_4100|M.gilvum_PYR-GCK          PDLMDILGPASVLPSGRS-------AEYLFRLALPMGIYGGTLDVFRNMI
Mvan_2246|M.vanbaalenii_PYR-1       PDLMDVLGTTSVVPEGLN-------AEYLFRLALPTGIYGGTLDVFRNMI
MSMEG_0578|M.smegmatis_MC2_155      PDLMDILGPAGALTDG---------VEYIFRLAGPTGIYGGTLEVFRNMI
TH_2587|M.thermoresistible__bu      PELMDVLGAASALPVDTTGAAADGAVEYLFRLASPVAIYGGTVEVFRNMI
MAV_5127|M.avium_104                QELMDLLGTASALPTDTPGSPDDGAIEFIFRHAVPAGIYGGTMEVFRNMI
MMAR_4010|M.marinum_M               PDLMDILGSAAALPFGTDGAVDDGGAEYVYRFAPLVGIYGGTLEVFRNMI
MUL_3874|M.ulcerans_Agy99           PDLMDILGSAAALPFGTDGAVDDGGAEYVYRFAPLVGIYGGTHEVFRNMI
MLBr_00737|M.leprae_Br4923          TDAVQLFGGAGYTSDFP--------VERFMRDAKITQIYEGTNQIQRVVM
                                        : .      .            :   :      :  *. :: * ::

Mb3573c|M.bovis_AF2122/97           AASGLKVPRVPR--------
Rv3543c|M.tuberculosis_H37Rv        AASGLKVPRVPR--------
MAB_0615|M.abscessus_ATCC_1997      STAGLGTPRVPR--------
Mflv_4100|M.gilvum_PYR-GCK          AQHALGLGKPNYSPPR----
Mvan_2246|M.vanbaalenii_PYR-1       AQHALGLGRPNYSPPVQRP-
MSMEG_0578|M.smegmatis_MC2_155      AQHGLGLGKPNYAQPVKKVS
TH_2587|M.thermoresistible__bu      AQHALGLGKPSYGTPVKR--
MAV_5127|M.avium_104                AQHELGLGRPSYGR------
MMAR_4010|M.marinum_M               AQHVLGLGKPSYPHRT----
MUL_3874|M.ulcerans_Agy99           AQHVLGLGKPSYPHRT----
MLBr_00737|M.leprae_Br4923          SRALLR--------------
                                    :   *

species	id	gene	e-value	identity (len)	annotation
M. smegmatis MC2 155	MSMEG_0578	-	-	100% (378)	acyl-CoA dehydrogenase middle domain-containing protein
M. smegmatis MC2 155	MSMEG_2234	-	e-146	64.65% (396)	acyl-CoA dehydrogenase middle domain-containing protein
M. smegmatis MC2 155	MSMEG_5993	-	1e-36	35.00% (240)	putative acyl-CoA dehydrogenase