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MNPLPTAWSGRLVLPVIAAPMTSVSGPELVIAACRAGVIGSFPTHNATSPEELDSWLRRITAQAGGGAPV APNLVVHRSNARLQADLDCLIRHRVELVITSVGSPAAVVDRLHGIGAQLYADVASLRHAWAALDAGADGL VLLTAGAGGQTGWANPLAFLSAVRERFDGPLVLAGGIADGRSILAAQAAGADLAYVGTRFIATSKSRASD EYRRAVVDSTLDDVKLSDRVGGIPASLLSAWLDRQADPEGASDGFAQDRLLSNGDAWSAGHSVSGVHGVT TVEELVAELAGQYQAARAEFAALCEAGGPLR
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. smegmatis MC2 155 | MSMEG_0332 | - | - | 100% (311) | 2-nitropropane dioxygenase, NPD |
| M. smegmatis MC2 155 | MSMEG_6004 | - | 2e-17 | 27.76% (245) | oxidoreductase, 2-nitropropane dioxygenase family protein |
| M. smegmatis MC2 155 | MSMEG_1660 | - | 9e-17 | 30.64% (235) | oxidoreductase, 2-nitropropane dioxygenase family protein |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb3583 | - | 3e-19 | 28.16% (245) | oxidoreductase |
| M. gilvum PYR-GCK | Mflv_1222 | - | 6e-22 | 33.05% (239) | 2-nitropropane dioxygenase, NPD |
| M. tuberculosis H37Rv | Rv3553 | - | 3e-19 | 28.16% (245) | oxidoreductase |
| M. leprae Br4923 | - | - | - | - | - |
| M. abscessus ATCC 19977 | MAB_4001c | - | 5e-18 | 32.29% (223) | putative 2-nitropropane dioxygenase, NPD |
| M. marinum M | MMAR_5042 | - | 6e-19 | 27.46% (244) | 2-nitropropane dioxygenase |
| M. avium 104 | MAV_2769 | - | 9e-22 | 36.18% (199) | oxidoreductase, 2-nitropropane dioxygenase family protein |
| M. thermoresistible (build 8) | TH_4534 | - | 8e-22 | 34.88% (215) | PUTATIVE oxidoreductase, 2-nitropropane dioxygenase family |
| M. ulcerans Agy99 | MUL_4116 | - | 5e-19 | 27.46% (244) | 2-nitropropane dioxygenase |
| M. vanbaalenii PYR-1 | Mvan_0347 | - | 1e-115 | 69.31% (303) | 2-nitropropane dioxygenase, NPD |
CLUSTAL 2.0.9 multiple sequence alignment
MSMEG_0332|M.smegmatis_MC2_155 -MNPLPT---AWSGRLVLPVIAAPMT-SVSGPELVIAACRAGVIGSFPTH
Mvan_0347|M.vanbaalenii_PYR-1 MHVPIPSRILPWAGRLRLPVIAAPMT-SVSTPELVIGACRSGAIGSFPTH
MMAR_5042|M.marinum_M --MRLRTPLTDLVG-VEHPVVQTGMG-WVAGARLVSATANAGGLGILASA
MUL_4116|M.ulcerans_Agy99 --MRLRTPLTDLVG-VEHPVVQTGMG-WVAGARLVSATANAGGLGILASA
Mb3583|M.bovis_AF2122/97 --MRLRTPLTELIG-IEHPVVQTGMG-WVAGARLVSATANAGGLGILASA
Rv3553|M.tuberculosis_H37Rv --MRLRTPLTELIG-IEHPVVQTGMG-WVAGARLVSATANAGGLGILASA
Mflv_1222|M.gilvum_PYR-GCK ----MTNRIQDLLG-VEFPVVQAPMT-YIARAELAAAVSDGGGLGMIETL
MAV_2769|M.avium_104 ------------------------MT-YIAGAQLAAAVSNAGALGIIETT
MAB_4001c|M.abscessus_ATCC_199 ---MITNRVTELLG-VERPIVQAPMG-WIARSQLASAVCNAGGLGIIETS
TH_4534|M.thermoresistible__bu ------VDVLDRLR-LDVPVAQAGMGGGLAGPELAVAVADAGGLGTLGLT
* :: ..*. ... .* :* :
MSMEG_0332|M.smegmatis_MC2_155 NATSPEELDSWLRRIT-AQAGG-GAPVAPNLVVHRSNARLQADLDCLIRH
Mvan_0347|M.vanbaalenii_PYR-1 NAATTRELDAWLARAKSELAAE-AAPVAPNLVVHRSNPRRDDDLAVLIHH
MMAR_5042|M.marinum_M TMT----LDELATAIGKVKATT-DKPFGVNIRAD--AADASDRVELMIRE
MUL_4116|M.ulcerans_Agy99 TMT----LDELATAIGKVKATT-DKPFGVNIRAD--AADASDRVELMIRE
Mb3583|M.bovis_AF2122/97 TMT----LDELAAAITKVKAVT-DKPFGVNIRAD--AADAGDRVELMIRE
Rv3553|M.tuberculosis_H37Rv TMT----LDELAAAITKVKAVT-DKPFGVNIRAD--AADAGDRVELMIRE
Mflv_1222|M.gilvum_PYR-GCK T-------DEGRADLLRVRDLT-DRPVAANLMIQG-WKRDPSIVDVLAAA
MAV_2769|M.avium_104 S-------DQGRADLRRVRELT-AGAVGANIALL--FNRDPAMLDLLVAN
MAB_4001c|M.abscessus_ATCC_199 SGE----LDAIKDEIRAMREFT-DKPFGVNIAQA--FVRDPSIAQFVVDQ
TH_4534|M.thermoresistible__bu AP------DRLRDGIRRVRAEAPGRAVAVNLLMP--FVRRP-HVRVCVEE
* ... *:
MSMEG_0332|M.smegmatis_MC2_155 RVELVITSVGSPAAVVDRLHGIGAQLYADVASLRHAWAALDAGADGLVLL
Mvan_0347|M.vanbaalenii_PYR-1 KVELVITSVGSPAPVIEPLHNAGARVFADVASLEHAWRALDAGADGLVLL
MMAR_5042|M.marinum_M GVKVASFALAPKPELIARLKEAGAVVIPSIGAAKHARKVAGWGADAMIVQ
MUL_4116|M.ulcerans_Agy99 GVKVASFALAPKPELIARLKEAGAVVIPSIGAAKHARKVAGWGADAMIVQ
Mb3583|M.bovis_AF2122/97 GVRVASFALAPKQQLIARLKEAGAVVIPSIGAAKHARKVAAWGADAMIVQ
Rv3553|M.tuberculosis_H37Rv GVRVASFALAPKQQLIARLKEAGAVVIPSIGAAKHARKVAAWGADAMIVQ
Mflv_1222|M.gilvum_PYR-GCK GVRHVFTSAGDPALFTDRLHDAGMTVVHVIGSLKGALKAADAGVDALVVE
MAV_2769|M.avium_104 DIRFVTTSAGDPALFTDRLHDAGITVFHVVGTLAAAKKAVDAGVDGLVVE
MAB_4001c|M.abscessus_ATCC_199 GVTFVTTSAGDPNKYTRVLKDNGLTVFHVVPTLAAALKAVDAGVDGLVVE
TH_4534|M.thermoresistible__bu RIDVAVVAFGGDRALVRELRDAGVFVFTMVGTADQVRRALHWGVDALIAQ
: . : . *: * : : : . . *.*.::
MSMEG_0332|M.smegmatis_MC2_155 TAGAGGQTGW--ANPLAFLSAVRERFDG---PLVLAGGIADGRSILAAQA
Mvan_0347|M.vanbaalenii_PYR-1 TAGAGGQTGW--ANPFAFVRAVRENFDG---PVVLAGGISDGRAILAAQI
MMAR_5042|M.marinum_M GGEGGGHTGP--VATTLLLPSVLDAVKGTGIPVIAAGGFFDGRGLAAALS
MUL_4116|M.ulcerans_Agy99 GGEGGGHTGP--VATTLLLPSVLDAVKGTGIPVIAAGGFFDGHGLAAALS
Mb3583|M.bovis_AF2122/97 GGEGGGHTGP--VATTLLLPSVLDAVAGTGIPVIAAGGFFDGRGLAAALC
Rv3553|M.tuberculosis_H37Rv GGEGGGHTGP--VATTLLLPSVLDAVAGTGIPVIAAGGFFDGRGLAAALC
Mflv_1222|M.gilvum_PYR-GCK GVEGGGFKSALGASTMVLLPLVAERVD---LPLICAGGICDARSAAAAVV
MAV_2769|M.avium_104 GVEGGGFKNRFGASTMVLLPLVAAHVD---VPIVAAGGICDARSMAAAFV
MAB_4001c|M.abscessus_ATCC_199 GVEGGGFKDPKGASTMVLLPLVRSHVD---IPIIAAGGICDGVSMAAAFA
TH_4534|M.thermoresistible__bu GAEAGGHLVG-ESPAAAFLPKARDLAGG--RPVFLAGGVATAADTRAALD
.** . :: . *:. ***. . **
MSMEG_0332|M.smegmatis_MC2_155 AGADLAYVGTRFIATSKSRASDEYRRAVVDSTLDDVKLSDRVGG-IPASL
Mvan_0347|M.vanbaalenii_PYR-1 LGADFAYLGTRFIATRQSAAGDDYRDALVAATLDDVRLSDRVGG-VPASL
MMAR_5042|M.marinum_M YGAAGVAMGTRFLLTSDSTVPEAVKRRYLQAALDGTVVTTRVDG-MPHRV
MUL_4116|M.ulcerans_Agy99 YGAAGVAMGTRFLLTSDSTVPDAVKRRYLQAALDGTVVTTRVDG-MPHRV
Mb3583|M.bovis_AF2122/97 YGAAGVAMGTRFLLTSDSTVPDAVKRRYLQAGLDGTVVTTRVDG-MPHRV
Rv3553|M.tuberculosis_H37Rv YGAAGVAMGTRFLLTSDSTVPDAVKRRYLQAGLDGTVVTTRVDG-MPHRV
Mflv_1222|M.gilvum_PYR-GCK LGAEGIQMGTRMLASAEALVHANFKDAIVAADDAGTILLDVPGN-PTMRV
MAV_2769|M.avium_104 LGAEAVQMGTRLLASADSPVHVNLKQAVVAADETATVLLPLDGK-RMMRV
MAB_4001c|M.abscessus_ATCC_199 LGAEGVQMGTRMMSAAESPIHHNWKAAVVAARETDTVLLNRLTK-PGLRA
TH_4534|M.thermoresistible__bu AGADAVVAGTRFLLTHESGAHPEYQRRLLEAETTYRTMLFGLGWPAPHRV
** ***:: : .: : : : :
MSMEG_0332|M.smegmatis_MC2_155 LSAWLDRQADP--EGASDGFAQDRLLSNG---------------------
Mvan_0347|M.vanbaalenii_PYR-1 LSCWLDAQSTPGADGAGSGFTQKQLLANR---------------------
MMAR_5042|M.marinum_M LRTGLVEKLEGGSRARGFTAAIRNAAKFKQMSQMSWASMIKDGLAMRHGK
MUL_4116|M.ulcerans_Agy99 LRTGLVEKLEGGSRARGFTAAIRNAAKFKQMSQMSWASMIKDGLAMRHGK
Mb3583|M.bovis_AF2122/97 LRTELVEKLESGSRARGFAAALRNAGKFRRMSQMTWRSMIRDGLTMRHGK
Rv3553|M.tuberculosis_H37Rv LRTELVEKLESGSRARGFAAALRNAGKFRRMSQMTWRSMIRDGLTMRHGK
Mflv_1222|M.gilvum_PYR-GCK LRTGLAARIDEQDAD---AKLLGRITDLY---------------------
MAV_2769|M.avium_104 IRTPAAERLEAAGAAGEGAAALQRVQRLY---------------------
MAB_4001c|M.abscessus_ATCC_199 LRSARTEEMERRDLVS--LLETGDPLDLY---------------------
TH_4534|M.thermoresistible__bu VGNAATRRWCDASGTAKRLPVWINARSEFMARLG----------------
: .
MSMEG_0332|M.smegmatis_MC2_155 ----------------------------DAWSAGHSVSGVHGVTTVEELV
Mvan_0347|M.vanbaalenii_PYR-1 ----------------------------EAWSAGHSVSGVHGVVDVADLV
MMAR_5042|M.marinum_M ELTWSQVVMAANTPMLLKAGLVDGNPEAGVLASGQVAGILDDLPSCAQLV
MUL_4116|M.ulcerans_Agy99 ELTWSQVLMAANTPMLLKAGLVDGNPEAGVLASGQVAGILDDLPSCAQLV
Mb3583|M.bovis_AF2122/97 ELTWSQVLMAANTPMLLKAGLVDGNTEAGVLASGQVAGILDDLPSCKELI
Rv3553|M.tuberculosis_H37Rv ELTWSQVLMAANTPMLLKAGLVDGNTEAGVLASGQVAGILDDLPSCKELI
Mflv_1222|M.gilvum_PYR-GCK ---------------------FGGDLEASVANTGQVSSRITGLAPAAEIV
MAV_2769|M.avium_104 ---------------------FEGDLDASVANTGQVAGRIRDLPPAADII
MAB_4001c|M.abscessus_ATCC_199 ---------------------FGGNMETFVPLGGQVAGRIGGVESVKDIL
TH_4534|M.thermoresistible__bu -----------DVPPAVLVRWQRPALPLFSPAAPTRAMPAAWVDRTALYA
:
MSMEG_0332|M.smegmatis_MC2_155 AELAGQYQAARAEFAALCEAGGPLR-
Mvan_0347|M.vanbaalenii_PYR-1 EELLTGYTVARRELKLVIGN------
MMAR_5042|M.marinum_M ESIVHDAIEHLRAAAALVE-------
MUL_4116|M.ulcerans_Agy99 ESIVHDAIEHLRAAAALVE-------
Mb3583|M.bovis_AF2122/97 ESIVLDAITHLQTASALVE-------
Rv3553|M.tuberculosis_H37Rv ESIVLDAITHLQTASALVE-------
Mflv_1222|M.gilvum_PYR-GCK RQTWCEIQTVLDDTRARLG-------
MAV_2769|M.avium_104 KQMWKGCREVLAATSARLDWADDA--
MAB_4001c|M.abscessus_ATCC_199 DATMDEFTAVIGKLAAQYV-------
TH_4534|M.thermoresistible__bu GESVLRMAAVIPARDAVAELSPAQGV