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VSNATLPAESDVLVVGAGPAGMVTAALLERLGMAVRILERFPSRLALPKAHVVGPVTLDICVQAGLDVDR MSADAHPPEWDQFVRFATRLFGEQLGTIAYEYQSPEWTARPRINLSQPRFEAIVEGDVRQRPGIEWVCGR WTGAQVDKGKVVSTATVDDRQVQFSSRYLIGADGANSAVRESLGISMHTYGQPSHQINAHVVADMREQLT DHPAVLYALVDPEVAGVAVIHGLGEPGHDNTIEWICGSPYDGENVPEMDTIVDRVRKVIGSDDIGFEVLK LSPWVLTARVADQFQAGPCFLIGDAAHRLPPTGGLGMNTAIQDGANLAWKLAAVIHGWAGEALLQSYSTE RRPIAIANTLRSLENFEIHIQALAELATAALDGTLHTMAAERLDNLVQQNSIGFNSPGLQLGYGYGPNQE VADATSFTPSAAVGRRLPHAQVKTGAVDVVPIIDLVDRAGFTVFIQGAADPWREASGGSEFPVTIVDLAG AVADQGWLELTGLAHEGAALVVRPDGHIAAKAAGPQSWPGIHSSLRNHLQTGQPLGTEAIA
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. marinum M | MMAR_3384 | - | - | 100% (551) | hydroxylase |
| M. marinum M | MMAR_3439 | - | 9e-32 | 27.70% (556) | hydroxylase |
| M. marinum M | MMAR_1503 | - | 6e-25 | 28.37% (363) | FAD-dependent oxidoreductase |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | - | - | - | - | - |
| M. gilvum PYR-GCK | Mflv_2401 | mhpA | 1e-22 | 27.86% (359) | 3-(3-hydroxyphenyl)propionate hydroxylase |
| M. tuberculosis H37Rv | - | - | - | - | - |
| M. leprae Br4923 | - | - | - | - | - |
| M. abscessus ATCC 19977 | MAB_4357c | - | 6e-51 | 30.52% (557) | putative monooxygenase |
| M. avium 104 | MAV_2189 | - | 3e-34 | 29.76% (531) | FAD binding domain-containing protein |
| M. smegmatis MC2 155 | MSMEG_2252 | - | 4e-27 | 27.33% (516) | flavin-type hydroxylase |
| M. thermoresistible (build 8) | TH_2800 | - | 2e-52 | 30.35% (537) | PUTATIVE putative monooxygenase, FAD-binding |
| M. ulcerans Agy99 | MUL_3663 | - | 1e-10 | 24.22% (351) | hypothetical protein MUL_3663 |
| M. vanbaalenii PYR-1 | Mvan_4406 | - | 3e-45 | 28.40% (581) | monooxygenase, FAD-binding |
CLUSTAL 2.0.9 multiple sequence alignment
MAB_4357c|M.abscessus_ATCC_199 ----------------MSTVITPVVIVGAGPTGLATAYVLGRLGVRSIVC
TH_2800|M.thermoresistible__bu ---------------VSGPEQAPVLIVGAGPAGLATAYVLGRNGIPSVVC
MAV_2189|M.avium_104 -----------------MTTRVPVLIVGAGVGGLSMSALLTQHGVRSLLV
Mvan_4406|M.vanbaalenii_PYR-1 -MPEFNDGIAETPLPDSAVIETDVLIVGSGPAGASAALFLSTLGIPNIMI
MMAR_3384|M.marinum_M ------------MSNATLPAESDVLVVGAGPAGMVTAALLERLGMAVRIL
MSMEG_2252|M.smegmatis_MC2_155 MADAESLAAHGSRVAGKGISVKDVVIVGGGPTGAMLAGELRLYEVDVMVL
Mflv_2401|M.gilvum_PYR-GCK ---------------MTEQTDVDVVIVGAGPAGLTLANILGLEGVRVLVV
MUL_3663|M.ulcerans_Agy99 --------------MPQPSETTSVLVAGGGPAGMVLGLLLARAGVDVTVM
*::.*.* * . * : :
MAB_4357c|M.abscessus_ATCC_199 DQYDGVNPHPRAHVVNTRSMELLRNWGIYDEIIADAIDFNR--GINVLWK
TH_2800|M.thermoresistible__bu DQHSGIHPHPRAHVINTRSMELFRSWGIADAIARDEQAFDAGAGVRIVWK
MAV_2189|M.avium_104 EKRREVFLYPKARNLSFRSLEILRGMGLGDEVHAVAEHVST-----VVAK
Mvan_4406|M.vanbaalenii_PYR-1 TKYRWTADTPRAHITNQRAMEIFRDMGIEDQVLADATRHALVG--DTVFC
MMAR_3384|M.marinum_M ERFPSRLALPKAHVVGPVTLDICVQAGLDVDRMS-ADAHPPEWDQFVRFA
MSMEG_2252|M.smegmatis_MC2_155 DKDIEPTKVVRSLGLHARSIEIMDQRGLLDRFLEVGRRHPVG--------
Mflv_2401|M.gilvum_PYR-GCK DERDKLIDYPRGVGLDDESLRTFQAIGLVDRVLPHTVPNQILR-------
MUL_3663|M.ulcerans_Agy99 EKHADFLRDSRGDTVHASTLRLLDELGLGSQFAQIPHRLIDT--------
. :. :: *:
MAB_4357c|M.abscessus_ATCC_199 HTVAGEEFGRLDLADVPEAHLARRLNASPVTIGSCAQDRVQGRLLEAVRS
TH_2800|M.thermoresistible__bu HSVSGEELGCIDLLDAPVESIERRRAASPVGLISCPQDRIQRLMVDAVLR
MAV_2189|M.avium_104 PALNSPEE--RPALDVEGIFAGLERFSPEPAAQYCPQSRLEPILLAATRR
Mvan_4406|M.vanbaalenii_PYR-1 TSIAGEEIGRIHTWGTRPDREADYRLASPCELVDIPQTYLEPILVRNATS
MMAR_3384|M.marinum_M TRLFGEQLGTIAYEYQSPEWTARPRIN-------LSQPRFEAIVEGDVRQ
MSMEG_2252|M.smegmatis_MC2_155 GYFAGITKAPPRTLDTAHPYVLG-----------IPQPVTDRILVEHAVA
Mflv_2401|M.gilvum_PYR-GCK --FFDAKRNLLAEMAPPDARFGWPKRNG------FVQPMVDAELFGGLQR
MUL_3663|M.ulcerans_Agy99 --ISMKVQGQPVDLDLKHLPGAHQHIAL------VPQWDFLELLAAAAEA
. * :
MAB_4357c|M.abscessus_ATCC_199 SGMTEVRYRTKVSAVGA----HEDHVDVYLE--KDGHAETLRAEYVVDAE
TH_2800|M.thermoresistible__bu QGMSRIDYDTRVVEITD----AATGVEVTAE--SRGKPRRFRADYVVNAE
MAV_2189|M.avium_104 -GGSDVRYGTELSSIDM----DEAGVTAVVRDAESGTAETIRADYLIGAD
Mvan_4406|M.vanbaalenii_PYR-1 -RGTQTRFSTEYVSHAQ----DTTGVDVQVRDRLTGNQYTIRAKYLIGAD
MMAR_3384|M.marinum_M RPGIEWVCGRWTGAQVD----KGKVVSTATVD---DRQVQFSSRYLIGAD
MSMEG_2252|M.smegmatis_MC2_155 -SGAVLRRGCEAVGLAQ----DDDGVSVTLAD-----GTQVRARYVVGCD
Mflv_2401|M.gilvum_PYR-GCK FDNVEVRFGHRMHQCTQ----TTDRVEVEFDG----GQASVSARYVVGCD
MUL_3663|M.ulcerans_Agy99 EPTFRLLRSTEVTGVLWGDGPDPGRVVSATYRAQSGETKHMHADLTVACD
* . : : .:
MAB_4357c|M.abscessus_ATCC_199 GAAARLRQSIGIDVEGIPEFGHQIN-MYFHADLSEWTDQESE-LLFWVLN
TH_2800|M.thermoresistible__bu GAGGRLRSALGIEMDGIPFIADQVT-SYFHADLSEWTGERPP-LLQWIIN
MAV_2189|M.avium_104 GVHSPLRNLLGITTSGYGALPIYVVFVYFRAPWRKFIPHLHDGDGVQVKN
Mvan_4406|M.vanbaalenii_PYR-1 GARSKVAADIGLPFEGAMDIAGSMN-ITFKADIASHVAHRPSVLYWVIQP
MMAR_3384|M.marinum_M GANSAVRESLGISMHTYGQPSHQIN-AHVVADMREQLTDHPAVLYALVDP
MSMEG_2252|M.smegmatis_MC2_155 GGRSTVRKLMGVGFPGEPSRVDTLIGEMQVGVTPEQVTAVVAEVRKMQLR
Mflv_2401|M.gilvum_PYR-GCK GGRSVTRRLMGVSFDGTTSSTRWLVVDIANDPLGHPNSEVGADPRRPYVS
MUL_3663|M.ulcerans_Agy99 GRGSTVRSALGMATRSFGAP---MDVWWFRLPRRPDDPTGLAGIFNSGHG
* . :*: :
MAB_4357c|M.abscessus_ATCC_199 ----TGCPGVFIRLGGNRWTFHTGFDPAR-EAVSDYTPERCRELIRLAVG
TH_2800|M.thermoresistible__bu ----TDVQGTFIAMGQNRWTFTIGFGATD-EARRALDPQRCVELIRLAIG
MAV_2189|M.avium_104 ----ADVDGIFLVAEGDLGMFITTYFPDEGETAEQFTPQRCRELLTKAIG
Mvan_4406|M.vanbaalenii_PYR-1 GANVGGIGAGLVRMVRPWNEWLIVWGYDINDEPPALDEDEAVNIVRQLLG
MMAR_3384|M.marinum_M --EVAGVAVIHGLGEPGHDNTIEWICGSPYDGENVPEMDTIVDRVRKVIG
MSMEG_2252|M.smegmatis_MC2_155 --------FGAGPLGDGLYRVMVPAAEVVEDRSATPTLDEFRQQLRATAG
Mflv_2401|M.gilvum_PYR-GCK -----------IAIAHGIRRFEFMIHPDETDEEADDP-----AFVRRMLG
MUL_3663|M.ulcerans_Agy99 ---------GIAIDRGDYYQIAYIIPKGSDAQLRAQGIEALHRALADLMP
:
MAB_4357c|M.abscessus_ATCC_199 --APELEIDMR--SVGTWVLGASTARQYRKGRVFLVGDAAHQFPPTGGLG
TH_2800|M.thermoresistible__bu PAGSDVDIDFR--SAGSWTLCATTARTYRAGRVFLVGDAAHQFPPTGGFG
MAV_2189|M.avium_104 ---EPLDIDVI--EVAAWQPYEQVADRFDCGRAFLVGDSAHTMPPFKAGG
Mvan_4406|M.vanbaalenii_PYR-1 --ISDLPVEIT--GASLWGNNEMYATHLQQGRVFCAGDAVHRHPPSNGLG
MMAR_3384|M.marinum_M --SDDIGFEVL--KLSPWVLTARVADQFQAGPCFLIGDAAHRLPPTGGLG
MSMEG_2252|M.smegmatis_MC2_155 ---TDLGVHSPR-WLSRFGDGTRLADRYRTGRVLLAGDAAHVHPPVGGQG
Mflv_2401|M.gilvum_PYR-GCK QLIPYPERVDMI-RHRVYTHHSRIAGSFREGRLMLAGDAAHLMPVWQGQG
MUL_3663|M.ulcerans_Agy99 WLADRVDALKSFDDVKLLDVQLNRLRRWYCDGALCIGDAAHAMSPVGGVG
. : **:.* . . *
MAB_4357c|M.abscessus_ATCC_199 MNTGLVDADNLGWKLAAVLQG-WAHTQLLDTYESERRPVALANAESSIAN
TH_2800|M.thermoresistible__bu MNTGLADADNLGWKLAAVLHG-WADEELLDTYESERRPVALANSEFSVTN
MAV_2189|M.avium_104 ANTAIQSAHNLAWKLAAVLRG-AAGPALLDTYHAERHPVGVFAARQSLT-
Mvan_4406|M.vanbaalenii_PYR-1 SNTSIQDSYNLAWKIAAVLRG-QADPALLETYSAERAPIAEQIVKRANKS
MMAR_3384|M.marinum_M MNTAIQDGANLAWKLAAVIHG-WAGEALLQSYSTERRPIAIANTLRSLEN
MSMEG_2252|M.smegmatis_MC2_155 LNLGVQDAFNLGWKLAASING-WAPDGLLDSYEAERRPVAAAVLDNTRAQ
Mflv_2401|M.gilvum_PYR-GCK YNSGIRDAANLGWKLAAVVTG-RAGEELLDTYDVERRKHARAMIDLSTMV
MUL_3663|M.ulcerans_Agy99 INLALADAVATARMLAPSLRVGRVSTGQLARVQARRWAPTAFLQGIQRAI
* .: .. . :*. : . * .*
MAB_4357c|M.abscessus_ATCC_199 VMKMADAGIGP--------NTMAVAAKLESDIPAVAAAERSRMAAAVPEQ
TH_2800|M.thermoresistible__bu ALKMASTGIGP--------TGPQFAAELESDDPSVAAAVRDRIRNAIPEQ
MAV_2189|M.avium_104 GPSMALLRPGG--------KRPQLPADEEAP-------------------
Mvan_4406|M.vanbaalenii_PYR-1 SREYGQFFEVLGLLDANDEQEMAARIEERKAATPRGAAMREALVAAMELK
MMAR_3384|M.marinum_M FEIHIQALAEL--------ATAALDGTLHTMAAER-------LDNLVQQN
MSMEG_2252|M.smegmatis_MC2_155 MELLSPEPGPQ--------AVRRLLAQLMAFEEVN--------------R
Mflv_2401|M.gilvum_PYR-GCK GRVISPTNRKVAALRDRVIHAASAVPSLKRYVLEMRFKPMPRYQQGAVLH
MUL_3663|M.ulcerans_Agy99 HAAIVAVAVTG---------------------------------------
MAB_4357c|M.abscessus_ATCC_199 RPHFDYLELELGYVYGGHNDAVDP--------------------ITTAVM
TH_2800|M.thermoresistible__bu RPHFDHLELEIGYVYPGDASDGDP--------------------VSTPVV
MAV_2189|M.avium_104 -----IFALMIGYRYGANGIELVD--------------------ELRAQP
Mvan_4406|M.vanbaalenii_PYR-1 NYEFNAHGVDLGQTYQSAAVVSEGSTLPIPARDPE------LFYEMSTVP
MMAR_3384|M.marinum_M SIGFNSPGLQLGYGYGPNQEVADATS-----------------FTPSAAV
MSMEG_2252|M.smegmatis_MC2_155 YLTEMITATGIRYDFGAEDD----------------------------LV
Mflv_2401|M.gilvum_PYR-GCK HAHAAPNSPTGTLFIQPRVDTRDTQNALLDDVLGTGFAVVCWSNNLRAVL
MUL_3663|M.ulcerans_Agy99 --------------------------------------------------
MAB_4357c|M.abscessus_ATCC_199 GGRMPHHWVN---LGDRRISTLDLVG-----VGFVLIAGPEGAEWIDAPK
TH_2800|M.thermoresistible__bu GGRLPHRWITRITPGQGTASTLDLLG-----DGFTLLTGPAGAAWTAG--
MAV_2189|M.avium_104 GTRVPHVWVR------DGVSTLDLVG-----PEFTVLTGDE---------
Mvan_4406|M.vanbaalenii_PYR-1 GSHLPHAWVG---DSRTKLALMDLAPY----TEWTVITGIAGEAWAGAAD
MMAR_3384|M.marinum_M GRRLPHAQVKTG--AVDVVPIIDLVDR----AGFTVFIQGAADPWREASG
MSMEG_2252|M.smegmatis_MC2_155 GRRLRDIRLR-------RGRLYELTR-----TGRGLLLDQTG--------
Mflv_2401|M.gilvum_PYR-GCK GEEAFGRWKALGAKFVEVRPMSQLRWPGHDDPDVAVIGDRTGALKAWFDV
MUL_3663|M.ulcerans_Agy99 GPPQPPPVVRLVSR------------------------------------
*
MAB_4357c|M.abscessus_ATCC_199 --IREMRIPLQVYLVG----RDLEPIG-GAIPGAEGN-GAVLVRPDGHIA
TH_2800|M.thermoresistible__bu --VRDVAVPVRTLTAG----VDFAFTGPSDTTFLADD-GAVLVRPDGHIA
MAV_2189|M.avium_104 ---RWCAAAASMSLPA----HRVYNDQWAAITGLAPD-GALLIRPDDFVG
Mvan_4406|M.vanbaalenii_PYR-1 KVGHELGIPLRTVVIGPGREVTDLYYDWAKLREVDED-GALLVRPDKHIA
MMAR_3384|M.marinum_M ----GSEFPVTIVDLAG----AVADQGWLELTGLAHEGAALVVRPDGHIA
MSMEG_2252|M.smegmatis_MC2_155 ----------QLSAAG-----WSDRVDHVVDVSDELDVPAVLLRPDGHIA
Mflv_2401|M.gilvum_PYR-GCK HTESVLFVRPDRCIAAACIAQRAPEISEGLFAALHLTSGAHLTQGGGTDG
MUL_3663|M.ulcerans_Agy99 --------------------------------------SVPVRRIMGYLV
. : :
MAB_4357c|M.abscessus_ATCC_199 WIGTGLAVDPVEEISLAVETLCGKPLSEIAT-------
TH_2800|M.thermoresistible__bu WIATHLPADPAAELTSALRGVHLSADAARSASGQGASR
MAV_2189|M.avium_104 WRADELPTDPQTALRQALSLCLGRAR------------
Mvan_4406|M.vanbaalenii_PYR-1 WRSMSMPDQPHAALRSAMAQILGRD-------------
MMAR_3384|M.marinum_M AKAAGPQSWPGIHSSLRNHLQTGQPLGTEAIA------
MSMEG_2252|M.smegmatis_MC2_155 WVGDE-----QGDLEGCLVRWFGVPA------------
Mflv_2401|M.gilvum_PYR-GCK EKPDRAVLHVAQPAAESSGTGAGTS-------------
MUL_3663|M.ulcerans_Agy99 AIGPLPEHAPKYPRRAR---------------------