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PEFNDGIAETPLPDSAVIETDVLIVGSGPAGASAALFLSTLGIPNIMITKYRWTADTPRAHITNQRAMEI FRDMGIEDQVLADATRHALVGDTVFCTSIAGEEIGRIHTWGTRPDREADYRLASPCELVDIPQTYLEPIL VRNATSRGTQTRFSTEYVSHAQDTTGVDVQVRDRLTGNQYTIRAKYLIGADGARSKVAADIGLPFEGAMD IAGSMNITFKADIASHVAHRPSVLYWVIQPGANVGGIGAGLVRMVRPWNEWLIVWGYDINDEPPALDEDE AVNIVRQLLGISDLPVEITGASLWGNNEMYATHLQQGRVFCAGDAVHRHPPSNGLGSNTSIQDSYNLAWK IAAVLRGQADPALLETYSAERAPIAEQIVKRANKSSREYGQFFEVLGLLDANDEQEMAARIEERKAATPR GAAMREALVAAMELKNYEFNAHGVDLGQTYQSAAVVSEGSTLPIPARDPELFYEMSTVPGSHLPHAWVGD SRTKLALMDLAPYTEWTVITGIAGEAWAGAADKVGHELGIPLRTVVIGPGREVTDLYYDWAKLREVDEDG ALLVRPDKHIAWRSMSMPDQPHAALRSAMAQILGRD*
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. vanbaalenii PYR-1 | Mvan_4406 | - | - | 100% (597) | monooxygenase, FAD-binding |
| M. vanbaalenii PYR-1 | Mvan_4245 | mhpA | 7e-24 | 25.52% (384) | 3-(3-hydroxyphenyl)propionate hydroxylase |
| M. vanbaalenii PYR-1 | Mvan_0593 | - | 4e-22 | 26.67% (390) | monooxygenase, FAD-binding |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | - | - | - | - | - |
| M. gilvum PYR-GCK | Mflv_2401 | mhpA | 1e-21 | 25.27% (368) | 3-(3-hydroxyphenyl)propionate hydroxylase |
| M. tuberculosis H37Rv | - | - | - | - | - |
| M. leprae Br4923 | - | - | - | - | - |
| M. abscessus ATCC 19977 | MAB_4357c | - | 4e-61 | 29.66% (590) | putative monooxygenase |
| M. marinum M | MMAR_3384 | - | 3e-45 | 28.40% (581) | hydroxylase |
| M. avium 104 | MAV_2189 | - | 5e-50 | 29.02% (579) | FAD binding domain-containing protein |
| M. smegmatis MC2 155 | MSMEG_2252 | - | 2e-31 | 31.04% (364) | flavin-type hydroxylase |
| M. thermoresistible (build 8) | TH_3923 | - | 7e-81 | 32.83% (594) | PUTATIVE putative monooxygenase, FAD-binding |
| M. ulcerans Agy99 | - | - | - | - | - |
CLUSTAL 2.0.9 multiple sequence alignment
MAV_2189|M.avium_104 -----------------MTTRVPVLIVGAGVGGLSMSALLTQHGVRSLLV
TH_3923|M.thermoresistible__bu ----------------MADLTVPVLVVGGGGCGLTTSILLSSHGIQHHLV
Mvan_4406|M.vanbaalenii_PYR-1 -MPEFNDGIAETPLPDSAVIETDVLIVGSGPAGASAALFLSTLGIPNIMI
MAB_4357c|M.abscessus_ATCC_199 ----------------MSTVITPVVIVGAGPTGLATAYVLGRLGVRSIVC
MMAR_3384|M.marinum_M ------------MSNATLPAESDVLVVGAGPAGMVTAALLERLGMAVRIL
MSMEG_2252|M.smegmatis_MC2_155 MADAESLAAHGSRVAGKGISVKDVVIVGGGPTGAMLAGELRLYEVDVMVL
Mflv_2401|M.gilvum_PYR-GCK ---------------MTEQTDVDVVIVGAGPAGLTLANILGLEGVRVLVV
*::**.* * : * : :
MAV_2189|M.avium_104 EKRREVFLYPKARNLSFRSLEILRGMGLG-DEVHAVAEHVSTVVAKPALN
TH_3923|M.thermoresistible__bu EQHSGTSPLPKAHYLNQRTMEVFRQYGLA-DSIYAVGTPPKNMGRTRWVT
Mvan_4406|M.vanbaalenii_PYR-1 TKYRWTADTPRAHITNQRAMEIFRDMGIE-DQVLADATRHALVGDTVFCT
MAB_4357c|M.abscessus_ATCC_199 DQYDGVNPHPRAHVVNTRSMELLRNWGIY-DEIIADAIDFNRGINVLWKH
MMAR_3384|M.marinum_M ERFPSRLALPKAHVVGPVTLDICVQAGLDVDRMSADAHPPEWDQFVRFAT
MSMEG_2252|M.smegmatis_MC2_155 DKDIEPTKVVRSLGLHARSIEIMDQRGLL-DRFLEVGRRHPVGGYFAGIT
Mflv_2401|M.gilvum_PYR-GCK DERDKLIDYPRGVGLDDESLRTFQAIGLV-DRVLPHTVPNQILRFFDAKR
. :. :: *: * .
MAV_2189|M.avium_104 SPEERPALDVEGIFAGLERFS-----------PEPAAQYCPQSRLEPILL
TH_3923|M.thermoresistible__bu SLGGDGELDARTLFT-LESFGGGGLENKYAQDSPCLSSNYPQIRLEPLLR
Mvan_4406|M.vanbaalenii_PYR-1 SIAGEEIGRIHTWGTRPDREADYR------LASPCELVDIPQTYLEPILV
MAB_4357c|M.abscessus_ATCC_199 TVAGEEFGRLDLADVPEAHLARRLN------ASPVTIGSCAQDRVQGRLL
MMAR_3384|M.marinum_M RLFGEQLGTIAYEYQSPEWTARPR-------------INLSQPRFEAIVE
MSMEG_2252|M.smegmatis_MC2_155 KAPPRTLDTAH-----------------------PYVLGIPQPVTDRILV
Mflv_2401|M.gilvum_PYR-GCK NLLAEMAPPDARFG-------------------WPKRNGFVQPMVDAELF
* : :
MAV_2189|M.avium_104 AATRRGGS-DVRYGTELSSIDMDEAGVTAVVRDAESGTAETIRADYLIGA
TH_3923|M.thermoresistible__bu DHAERRAPGAIHFNHKLMSFTLDDEGVEATILDQDNQRTYTVRAQYMVAA
Mvan_4406|M.vanbaalenii_PYR-1 RNATSRGT-QTRFSTEYVSHAQDTTGVDVQVRDRLTGNQYTIRAKYLIGA
MAB_4357c|M.abscessus_ATCC_199 EAVRSSGMTEVRYRTKVSAVGAHEDHVDVYLEK--DGHAETLRAEYVVDA
MMAR_3384|M.marinum_M GDVRQRPGIEWVCGRWTGAQVDKGKVVSTATVD---DRQVQFSSRYLIGA
MSMEG_2252|M.smegmatis_MC2_155 EHAVASGA-VLRRGCEAVGLAQDDDGVSVTLADG-----TQVRARYVVGC
Mflv_2401|M.gilvum_PYR-GCK GGLQRFDNVEVRFGHRMHQCTQTTDRVEVEFDGG----QASVSARYVVGC
* . . : *:: .
MAV_2189|M.avium_104 DG---VHSPLRNLLGITTSGYGALPIYVVFVYFRAPWR--KFIPHLHDGD
TH_3923|M.thermoresistible__bu DGGK-TIGPALGVVMEGPSGILDMVSTHFSADLSQYWEDDVLITWLLNPE
Mvan_4406|M.vanbaalenii_PYR-1 DGARSKVAADIGLPFEGAMDIAGSMNITFKADIASHVAHRPSVLYWVIQP
MAB_4357c|M.abscessus_ATCC_199 EGAAARLRQSIGIDVEGIPEFGHQINMYFHADLSEWTDQESELLFWVLNT
MMAR_3384|M.marinum_M DGANSAVRESLGISMHTYGQPSHQINAHVVADMREQLTDHPAVLYALVDP
MSMEG_2252|M.smegmatis_MC2_155 DGGRSTVRKLMGVGFPGEPSRVDTLIGEMQVGVTPEQVTAVVAEVRKMQL
Mflv_2401|M.gilvum_PYR-GCK DGGRSVTRRLMGVSFDGTTSSTRWLVVDIANDPLGHPNSEVGADPRR---
:* .: .
MAV_2189|M.avium_104 GVQVKNADVDGIFLVAEGDLGMFITTYFPDEGETAEQFTPQRCRELLTKA
TH_3923|M.thermoresistible__bu GAGSWHSGAMAAMGPTWGKHSEEFVIHFTFRPDDPARFDEEAIVPRLREL
Mvan_4406|M.vanbaalenii_PYR-1 GANVG--GIGAGLVRMVRPWNEWLIVWGYDINDEPPALDEDEAVNIVRQL
MAB_4357c|M.abscessus_ATCC_199 G--------CPGVFIRLGGNRWTFHTGFDPAREAVSDYTPERCRELIRLA
MMAR_3384|M.marinum_M EVAG----VAVIHGLGEPGHDNTIEWICGSPYDGENVPEMDTIVDRVRKV
MSMEG_2252|M.smegmatis_MC2_155 RFGAG---------PLGDGLYRVMVPAAEVVEDRSATPTLDEFRQQLRAT
Mflv_2401|M.gilvum_PYR-GCK ----------PYVSIAIAHGIRRFEFMIHPDETDEEADDPAFVRRMLGQL
: :
MAV_2189|M.avium_104 IGEP-LDIDVIEVAAWQPYEQVADRFDCGRAFLVGDSAHTMPPFKAGGAN
TH_3923|M.thermoresistible__bu LKLPNLKPKVHKVSHWILEGVLADRYQVGRVFLAGDAAHRHPPTTGLGLN
Mvan_4406|M.vanbaalenii_PYR-1 LGISDLPVEITGASLWGNNEMYATHLQQGRVFCAGDAVHRHPPSNGLGSN
MAB_4357c|M.abscessus_ATCC_199 VGAPELEIDMRSVGTWVLGASTARQYRKGRVFLVGDAAHQFPPTGGLGMN
MMAR_3384|M.marinum_M IGSDDIGFEVLKLSPWVLTARVADQFQAGPCFLIGDAAHRLPPTGGLGMN
MSMEG_2252|M.smegmatis_MC2_155 AGTDLGVHSPRWLSRFGDGTRLADRYRTGRVLLAGDAAHVHPPVGGQGLN
Mflv_2401|M.gilvum_PYR-GCK IPYP-ERVDMIRHRVYTHHSRIAGSFREGRLMLAGDAAHLMPVWQGQGYN
. : * * : **:.* * . * *
MAV_2189|M.avium_104 TAIQSAHNLAWKLAAVLRGAAGPALLDTYHAERHPVGVFAARQSLTGPSM
TH_3923|M.thermoresistible__bu TAIQDAHNLVWKLAAVLRGDAGPALLETYESERRPVGMRNVDWAMFTFLN
Mvan_4406|M.vanbaalenii_PYR-1 TSIQDSYNLAWKIAAVLRGQADPALLETYSAERAPIAEQIVKRANKSSRE
MAB_4357c|M.abscessus_ATCC_199 TGLVDADNLGWKLAAVLQGWAHTQLLDTYESERRPVALANAESSIAN--V
MMAR_3384|M.marinum_M TAIQDGANLAWKLAAVIHGWAGEALLQSYSTERRPIAIANTLRSLENFEI
MSMEG_2252|M.smegmatis_MC2_155 LGVQDAFNLGWKLAASINGWAPDGLLDSYEAERRPVAAAVLDNTRAQMEL
Mflv_2401|M.gilvum_PYR-GCK SGIRDAANLGWKLAAVVTGRAGEELLDTYDVERRKHARAMIDLSTMVGRV
.: .. ** **:** : * * **::* ** . :
MAV_2189|M.avium_104 ALLRPGGK--RPQLPADEEAPIF---------------------------
TH_3923|M.thermoresistible__bu HLTIDAGLGLSPDQPLEARVQVFRDY------FSDTPMGETRRARAFEVV
Mvan_4406|M.vanbaalenii_PYR-1 YGQFFEVLGLLDANDEQEMAARIEER------KAATPRGAAMREALVAAM
MAB_4357c|M.abscessus_ATCC_199 MKMADAGIGPNTMAVAAKLESDIP------------AVAAAERSRMAAAV
MMAR_3384|M.marinum_M HIQALAELATAALDGTLHTMAAER---------------------LDNLV
MSMEG_2252|M.smegmatis_MC2_155 LSPEPGPQAVRRLLAQLMAFEEVN--------------------------
Mflv_2401|M.gilvum_PYR-GCK ISPTNRKVAALRDRVIHAASAVPSLKRYVLEMRFKPMPRYQQGAVLHHAH
MAV_2189|M.avium_104 ----------ALMIGYRYG--------------ANGIELVDELRAQPGTR
TH_3923|M.thermoresistible__bu NTQRTEFQAHDLEIGFSYEQGALVPDG-TQPPPRDPMGAVYHPTTRPGHR
Mvan_4406|M.vanbaalenii_PYR-1 ELKNYEFNAHGVDLGQTYQSAAVVSEGSTLPIPARDPELFYEMSTVPGSH
MAB_4357c|M.abscessus_ATCC_199 PEQRPHFDYLELELGYVYG--------------GHNDAVDPITTAVMGGR
MMAR_3384|M.marinum_M QQNSIGFNSPGLQLGYGYG-----------PNQEVADATSFTPSAAVGRR
MSMEG_2252|M.smegmatis_MC2_155 --RYLTEMITATGIRYDFG----------------------AEDDLVGRR
Mflv_2401|M.gilvum_PYR-GCK AAPNSPTGTLFIQPRVDTRDTQNALLDDVLGTGFAVVCWSNNLRAVLGEE
* .
MAV_2189|M.avium_104 VPHVWVRDG---VSTLDLVGP--------EFTVLTG-------------D
TH_3923|M.thermoresistible__bu LPHVWLERDGRRISTHDLTGAGE------RFALITGPTIGPWAEAAQLAE
Mvan_4406|M.vanbaalenii_PYR-1 LPHAWVGDSRTKLALMDLAPYT-------EWTVITGIAGEAWAGAADKVG
MAB_4357c|M.abscessus_ATCC_199 MPHHWVNLGDRRISTLDLVGVG--------FVLIAGPEGAEWIDAP--KI
MMAR_3384|M.marinum_M LPHAQVKTGAVDVVPIIDLVDRA------GFTVFIQGAADPWREAS----
MSMEG_2252|M.smegmatis_MC2_155 LRDIRLRRG--RLYELTRTGRG----------LLLDQTG-----------
Mflv_2401|M.gilvum_PYR-GCK AFGRWKALGAKFVEVRPMSQLRWPGHDDPDVAVIGDRTGALKAWFDVHTE
. : ::
MAV_2189|M.avium_104 ERWCAAAASMSLPAHRVYND---QWAAITGLAPD-GALLIRPDDFVGWRA
TH_3923|M.thermoresistible__bu EKYRVQIVVVAVGNGGEYRDPTGTWARVRGYEDG-GAVLVRPDNHVGWRC
Mvan_4406|M.vanbaalenii_PYR-1 HELGIPLRTVVIGPGREVTDLYYDWAKLREVDED-GALLVRPDKHIAWRS
MAB_4357c|M.abscessus_ATCC_199 REMRIPLQVYLVGRDLEPIG-----GAIPGAEGN-GAVLVRPDGHIAWIG
MMAR_3384|M.marinum_M GGSEFPVTIVDLAGAVADQG----WLELTGLAHEGAALVVRPDGHIAAKA
MSMEG_2252|M.smegmatis_MC2_155 -QLSAAGWSDRVDHVVDVSD-----------ELDVPAVLLRPDGHIAWVG
Mflv_2401|M.gilvum_PYR-GCK SVLFVRPDRCIAAACIAQRAPEISEGLFAALHLTSGAHLTQGGGTDGEKP
* : : . .
MAV_2189|M.avium_104 DELPTDPQTALRQALSLCLGRAR------
TH_3923|M.thermoresistible__bu PAGIMSPGDELACALSNIVSR--------
Mvan_4406|M.vanbaalenii_PYR-1 MSMPDQPHAALRSAMAQILGRD-------
MAB_4357c|M.abscessus_ATCC_199 TGLAVDPVEEISLAVETLCGKPLSEIAT-
MMAR_3384|M.marinum_M AGPQSWPGIHSSLRNHLQTGQPLGTEAIA
MSMEG_2252|M.smegmatis_MC2_155 DE-----QGDLEGCLVRWFGVPA------
Mflv_2401|M.gilvum_PYR-GCK DRAVLHVAQPAAESSGTGAGTS-------
.