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VTFWSLIAEAARRGSPRPLLADEHGRSMTARQLYDAACVAAAALAERGVRPGAVVSWQLPTTLETMVLMA ALARLGAVQNPIIPVLRESEVRFITGQLNTEYFVAPGLWRGFDHGGLARALSAERGFEVITVDLAAPPAA GALRLPGADPDSLPAPPQSADEARWIYYSSGTTAAPKGIRHTDSSVIAGSAGVVGMVGATSSDVDPIAFP VAHIGGAAMLATALLTGMRLALFEAFDPAATPLAIAAHNPTFLGTATPFFVAYLEAQRAQGNRPLFPSLR GCLAGGAPITAELSRRVRDTFGVAGIANAWGMTEFPCATSPSLTAAPEVLDHTVGPPVPGVEVRVVDGAE NELAAGQEGELRLKGPQCFLGYADPTLDADAFDDQGWLRTGDLGLIDADGNVRVTGRTKDAIIRNAENIS ALEIENALAAHPAVADVAVIGIPDPRTGERVCAVVVPATADGVTLESLVQHCRSRGLSRYKHPEHLVVVD TLPRNQFGKVIKKDLRDAFG
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. avium 104 | MAV_2116 | - | - | 100% (510) | AMP-binding enzyme, putative |
| M. avium 104 | MAV_2571 | - | 1e-82 | 38.31% (509) | cyclohex-1-ene-1-carboxylate:CoA ligase |
| M. avium 104 | MAV_1839 | - | 1e-80 | 36.33% (523) | cyclohex-1-ene-1-carboxylate:CoA ligase |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb2405 | mbtA | 4e-52 | 31.38% (513) | bifunctional salicyl-AMP ligase/salicyl-S-arcp synthetase |
| M. gilvum PYR-GCK | Mflv_1784 | - | 2e-74 | 35.83% (508) | AMP-dependent synthetase and ligase |
| M. tuberculosis H37Rv | Rv2384 | mbtA | 4e-52 | 31.38% (513) | bifunctional salicyl-AMP ligase/salicyl-S-arcp synthetase |
| M. leprae Br4923 | MLBr_02546 | fadD2 | 4e-42 | 28.76% (532) | acyl-CoA synthetase |
| M. abscessus ATCC 19977 | MAB_2247c | - | 2e-53 | 33.33% (513) | bifunctional salicyl-AMP ligase/salicyl-S-arcp synthetase |
| M. marinum M | MMAR_0303 | - | 9e-71 | 35.21% (514) | acyl-CoA synthetase |
| M. smegmatis MC2 155 | MSMEG_5631 | - | 8e-64 | 34.31% (513) | AMP-dependent synthetase and ligase |
| M. thermoresistible (build 8) | TH_3874 | - | 1e-162 | 70.32% (401) | PUTATIVE AMP-binding enzyme, putative |
| M. ulcerans Agy99 | MUL_4126 | fadD3 | 3e-47 | 32.87% (502) | acyl-CoA synthetase |
| M. vanbaalenii PYR-1 | Mvan_4966 | - | 1e-73 | 35.18% (506) | AMP-dependent synthetase and ligase |
CLUSTAL 2.0.9 multiple sequence alignment
MAV_2116|M.avium_104 ------------------------------------------MTFWSLIA
TH_3874|M.thermoresistible__bu -------------------------------------------VPGTMRT
Mb2405|M.bovis_AF2122/97 ------MPPKAADGRRPSPDGGLGGFVPFPADRAASYRAAGYWSGRTLDT
Rv2384|M.tuberculosis_H37Rv ------MPPKAADGRRPSPDGGLGGFVPFPADRAASYRAAGYWSGRTLDT
MAB_2247c|M.abscessus_ATCC_199 -----MTQPRSTTTASSAATPALDGFVPFPSVRADEYRRAGYWTGNTVES
Mflv_1784|M.gilvum_PYR-GCK -------------------------------------------MTGTLVE
Mvan_4966|M.vanbaalenii_PYR-1 -------------------------------------------MT-TLAD
MSMEG_5631|M.smegmatis_MC2_155 -------------------------------MRAEDAYERGLWVRTTLAD
MMAR_0303|M.marinum_M ----------MGGPKVPQRRLIDGRWVRWDADRAAGAYAHRWWVRRTLAD
MUL_4126|M.ulcerans_Agy99 ----------------------------------------MTSDPRTVPT
MLBr_02546|M.leprae_Br4923 MTKLRHYLERGAAEVHYLRSIFESGALGFEPPLTYAAMAVAMRKWGDLAM
:
MAV_2116|M.avium_104 EAARRGSPRPLLADEHGR-----SMTARQLYDAACVAAAALAERGVR--P
TH_3874|M.thermoresistible__bu SVLNCRVTKPTSASRQIG-----STGFCTAPSRASATITTMVSRVVGSCQ
Mb2405|M.bovis_AF2122/97 VLSDAARRWPDRLAVADAGDRPGHGGLSYAELDQRADRAAAALHGLGITP
Rv2384|M.tuberculosis_H37Rv VLSDAARRWPDRLAVADAGDRPGHGGLSYAELDQRADRAAAALHGLGITP
MAB_2247c|M.abscessus_ATCC_199 LLRVSAARRPCHPAVIDE-----HRALSYRELDEAADNAAHAFLRLGIAP
Mflv_1784|M.gilvum_PYR-GCK ALRDAARDTPDRVLVRDD-----AVALTCAELLSAATGLAHALTRR-MPA
Mvan_4966|M.vanbaalenii_PYR-1 ALRDAARDTPDRILLRDD-----EASLSSAELLSAATNLAHALVRR-MPV
MSMEG_5631|M.smegmatis_MC2_155 TLAEAARDTPQRVLLIDG-----DVRLTCAELHSRAKALAHEMAAR-MPA
MMAR_0303|M.marinum_M ALREAAQSTPQRVALVDA-----DRQLDCQELHRQAMALAQHLRSR-MPA
MUL_4126|M.ulcerans_Agy99 ALDRLVRRLPNHDALVTD-----ERSFTVSELNDEVYRAAAALIELGVKP
MLBr_02546|M.leprae_Br4923 LPAMPARRSPHRAALIDE-----EGTLTYKELDRAAHALANGLIAKGVRG
* . :
MAV_2116|M.avium_104 GAVVSWQLPTTLETMVLMAALAR-LGAVQNPIIPVLRESEVRFITGQLNT
TH_3874|M.thermoresistible__bu DTTVPGPTPSAVRAAAVPAAASRNCAAVSRRPKSSSSTTRSGCSSAVRST
Mb2405|M.bovis_AF2122/97 GDRVLLQLPNGCQFAVALFALLR-AGAIPVMCLPGHRAAELGHFAAVSAA
Rv2384|M.tuberculosis_H37Rv GDRVLLQLPNGCQFAVALFALLR-AGAIPVMCLPGHRAAELGHFAAVSAA
MAB_2247c|M.abscessus_ATCC_199 GDRVLLQLPNRASFAVALFGLMR-AGAIPVMCLPGHRAAELSHFIEVADA
Mflv_1784|M.gilvum_PYR-GCK GSVVSFTVPNWHEAAIIYLGATL-AGMVVNPILPSLRERELSFILDDADS
Mvan_4966|M.vanbaalenii_PYR-1 GSVVSFAVPNWHEAAVIYLGATL-AGMVVNPILPSLRERELSFILADAGS
MSMEG_5631|M.smegmatis_MC2_155 GSVVSFMLPNWHEAAIVYLASTL-AGMVINPILPSLRDHELRFILDDAAS
MMAR_0303|M.marinum_M GSVVSFMLPNWHEAAVIYLASTL-AAMVANPVLPSLREHELRFILEDAGS
MUL_4126|M.ulcerans_Agy99 AARVGIWSPNTWHWVVACLAIHH-VGAAMVPLNTRYTADEAADILARTEA
MLBr_02546|M.leprae_Br4923 GDGIAILARNHRWFVIANYGCAR-VGARIILLNSEFSGPQIKEVSEREGA
: . . . . . :
MAV_2116|M.avium_104 EYFVAPGLWRGFDHGGLARALSAERGFEVITVDLAAPP---AAGALRLPG
TH_3874|M.thermoresistible__bu SPQNVSXTGRGHDHVALAKSLAAERDMRFMVIDHGADP---AGPALRLPV
Mb2405|M.bovis_AF2122/97 TGLVVADVASGFDYRPMARELVADHPTLRHVIVDGDPGP--FVSWAQLCA
Rv2384|M.tuberculosis_H37Rv TGLVVADVASGFDYRPMARELVADHPTLRHVIVDGDPGP--FVSWAQLCA
MAB_2247c|M.abscessus_ATCC_199 VGLVIPDSAGGFDYRALAVALSQRHPRLRTVVVEGDAGP--FASWTSLLK
Mflv_1784|M.gilvum_PYR-GCK RMIFVPESFGGHDYAAMLDRVAPGLPSPPEVVVVRGE----GRVTLADLL
Mvan_4966|M.vanbaalenii_PYR-1 RAIFIPQTFGGHDYAAMLDRVVAGLPSPPEVVVVRGEP---RRPALTDLV
MSMEG_5631|M.smegmatis_MC2_155 RMIFIPGEFGQHDYAAMLQRVCAHLPTPPQVVVVRGEP---GPHTAFTAL
MMAR_0303|M.marinum_M RMIFAPADFRGHDYTSMLRRVAAQLPSRPEVIEVRGQCPDGVSTFQSLLV
MUL_4126|M.ulcerans_Agy99 PVLFAMGHFLGSDRVAGLDRKALPALRHIVRIPIEEPDGTWDEFIETGTD
MLBr_02546|M.leprae_Br4923 KVIIYDDEYT---KVVSKAEPALGKLRALGVNPDEEEPPGSTDETLAELI
MAV_2116|M.avium_104 ADPDSLPAPPQSADEARWIYYSSGTTAAPKGIRHTDSSVIAGSAGVVGMV
TH_3874|M.thermoresistible__bu GETSSLPPAPASGDEARWIYYSSGTTAAPKGVCHTDRSVMAGSAGVVGMI
Mb2405|M.bovis_AF2122/97 QAGTGSPAPPADPGSPALLLVSGGTTGMPKLIPRTHDDYVFNATASAALC
Rv2384|M.tuberculosis_H37Rv QAGTGSPAPPADPGSPALLLVSGGTTGMPKLIPRTHDDYVFNATASAALC
MAB_2247c|M.abscessus_ATCC_199 SVEPQGPLPTVQTDTPALLLVSGGTTAAPKLIPRTHEDYVYNATQAARVC
Mflv_1784|M.gilvum_PYR-GCK AEPADGVLPAPRPTDTRMILYTSGTTGRPKGVLHTHESLAALIAQLGRFW
Mvan_4966|M.vanbaalenii_PYR-1 SEPRDGTLPDLDPAATRMILYTSGTTGRPKGVLHSHKSLGALIAQLGRYW
MSMEG_5631|M.smegmatis_MC2_155 GAP-GGDLPALDPDSVRMMMHTSGTTGRPKGVLHSHNSIHALICQLRENW
MMAR_0303|M.marinum_M GRETAAPLPTADPDAVRMVLYTSGTTGRPKGVLHTHNSIHALICQIRDHW
MUL_4126|M.ulcerans_Agy99 TGAVTARAAAVSPDDVSDILFTSGTTGRSKGVLCAHRQSLSASASWAANG
MLBr_02546|M.leprae_Br4923 ARSSSAPAPKVR-KRASIIILTSGTTGTPKGANRNTPASLAPLGGILSKV
. : :.***. .*
MAV_2116|M.avium_104 GATSSDVDPIAFPVAHIGG-AAMLATALLTG-----MRLALFEAFDPAAT
TH_3874|M.thermoresistible__bu GATSDDVNPIAFPISHIGG-AAMLAASLLTG-----MRLVLFDVFDPEAT
Mb2405|M.bovis_AF2122/97 RLSADDVYLVVLAAGHN----FPLACPGLLGAMTVGATAVFAPDPSPEAA
Rv2384|M.tuberculosis_H37Rv RLSADDVYLVVLAAGHN----FPLACPGLLGAMTVGATAVFAPDPSPEAA
MAB_2247c|M.abscessus_ATCC_199 GLTQDDVYLVALPAGHN----FPLACPGLLGAISTGATTVFLSDPSPESA
Mflv_1784|M.gilvum_PYR-GCK RIDRGDTFLVPSPIAHIGGSIYAFECPLLLG-----TEAVLMQRWDPDAA
Mvan_4966|M.vanbaalenii_PYR-1 RIDPGDTFLVPSPIAHIGGSIYAFECPLLLG-----TQAVLMQRWDPDAA
MSMEG_5631|M.smegmatis_MC2_155 MIDPGDTFLVPSPIAHIGGSIYAFECPLLLG-----TTAVLMQRWNADDG
MMAR_0303|M.marinum_M MVEPGDGFLVASPIAHIGGSIYAFECPLLLG-----TTAVLLDRWDADRA
MUL_4126|M.ulcerans_Agy99 KITADDRHLCINPFFHNFGYKAGILACLQTG-----ATLFPHLTFDPLRT
MLBr_02546|M.leprae_Br4923 PFRAHEVTLLPAPMFHALG-YLHATLAMFLG-----STLVLRRRFKPATV
: . * * ..
MAV_2116|M.avium_104 PLAIAAHNPTFLGTATPFFVAYLEAQRAQGNRPLFPSLRGCLAGGAPITA
TH_3874|M.thermoresistible__bu PLAIARHRPTLLGTATPFYVAYMAAQRRHGERPLFPALRACVAGGAPITP
Mb2405|M.bovis_AF2122/97 FAAIERHGVTVTALVPALAKLWAQSCEWEPVT--PKSLRLLQVGGSKLEP
Rv2384|M.tuberculosis_H37Rv FAAIERHGVTVTALVPALAKLWAQSCEWEPVT--PKSLRLLQVGGSKLEP
MAB_2247c|M.abscessus_ATCC_199 FATIARHRVSVTAVVPSLAQLWAQATAWEPVL--PETLRLLQVGGAKLGA
Mflv_1784|M.gilvum_PYR-GCK VGLMLEHRVTHMAGATPFLVGLLAAAQRAGTR--LPDLKVFICGGASVPP
Mvan_4966|M.vanbaalenii_PYR-1 VALMLRHRCTHMAGATPFLDGLLAAAERAGTR--LPDLKVFICGGASVPP
MSMEG_5631|M.smegmatis_MC2_155 VALMSEHHCTHMAGATPFLDQLLNAAERAGTR--LPDLKVFICGGASVPP
MMAR_0303|M.marinum_M VQLMTSKRCTHMAGATPFLEQLLAAAQRAGTR--LPDLKFFVCGGASVSP
MUL_4126|M.ulcerans_Agy99 LQAIERHRITVLPGPPTIYQTLLDHPARDDYD--LSSLRFAVTGAATVPV
MLBr_02546|M.leprae_Br4923 LEDIEKHQATAMVVVPVMLSRILDTLENLETKSDLSSLRVVFVSGSQLGA
: : : . : *: ..: :
MAV_2116|M.avium_104 ELSRRVRDTFGVAGIANAWGMTEFP-CATSPSLTAAPEVLDHTVGPPVPG
TH_3874|M.thermoresistible__bu ELGRRVRDTFGVAGIANSWGLTEFP-VATSPPPDADPAVLDHTVGPPVSG
Mb2405|M.bovis_AF2122/97 EDARRVRTALTPG-LQQVFGMAEGL-LNFTRIGDPPEVVEHTQGRPLCPA
Rv2384|M.tuberculosis_H37Rv EDARRVRTALTPG-LQQVFGMAEGL-LNFTRIGDPPEVVEHTQGRPLCPA
MAB_2247c|M.abscessus_ATCC_199 DDARAVRESLTQG-LQQVFGMAEGL-LCLTQPGDPTDILDNSQGRPMCDA
Mflv_1784|M.gilvum_PYR-GCK TLIRSAAAYFDKAAVSRVYGSTEVP-VTTVGALD--DIDRAADTDGRPGI
Mvan_4966|M.vanbaalenii_PYR-1 SLIRRAADYFDKAAVSRVYGSTEVP-VTTVGSLD--DVDRAADTDGRPGI
MSMEG_5631|M.smegmatis_MC2_155 SLIRRASTCFEKAVVSRVYGSTEVP-VTTVGSIEPGDVEHAACTDGRPGL
MMAR_0303|M.marinum_M SLIRCATDYFDKAVVTRVYGSTEVP-VTTIGAPD--SSAHAAATDGRVGA
MUL_4126|M.ulcerans_Agy99 VLVERMQSELDIDIVLTAYGLTEANGMGTMCRADDDALTVATTCGRPFAD
MLBr_02546|M.leprae_Br4923 ELATRALEELGPV-IYNMYGSTEIAFATIAGPKD--LQINSATVGPVVKG
: : :* :*
MAV_2116|M.avium_104 VEVRVVDGAENELAAGQEGELRLKGPQCFLGYADP-TLDADAFDDQGWLR
TH_3874|M.thermoresistible__bu VRVRVVGDDGREVEPGEEGELRLKGPQCFLGYVDS-ALDDDAFDADGWFR
Mb2405|M.bovis_AF2122/97 DELRIVNADGEPVGPGEEGELLVRGPYTLNGYFAAERDNERCFDPDGFYR
Rv2384|M.tuberculosis_H37Rv DELRIVNADGEPVGPGEEGELLVRGPYTLNGYFAAERDNERCFDPDGFYR
MAB_2247c|M.abscessus_ATCC_199 DEVRIVDEDGADVPAGETGELLVRGPYTLNGYYRADADNMRSFTVDGFYR
Mflv_1784|M.gilvum_PYR-GCK AEVRIVD-----------GEIRARGPQMFTGYLHP-EDDRESFDDAGYFR
Mvan_4966|M.vanbaalenii_PYR-1 ADVTLVD-----------GEIRARGPQMLTGYLHP-EDNSESFDAEGFFR
MSMEG_5631|M.smegmatis_MC2_155 AEIRLVD-----------GEIRARGPQMLLGYLHA-EDN--AFDEQGFFR
MMAR_0303|M.marinum_M ADVKLVA-----------GEIRVRGPQMLVGYRHP-EDEAGSFDAQGYFR
MUL_4126|M.ulcerans_Agy99 FELRIGP---------DTGEVLLRGPNVMLGYLDDPRATAAAIDADGWLH
MLBr_02546|M.leprae_Br4923 VKVKILDDSGKEVPRGQVGRIFVGNAFPFEGYTGG----GGKQIIDGLLS
: : *.: .. : ** *
MAV_2116|M.avium_104 TGDLGLIDADGNVRVTGRTKDAIIRNAENISALEIENALAAHPAVADVAV
TH_3874|M.thermoresistible__bu SGDRGRIDAAGNVVVTGRIKDAIIRNAENISALEIEGVLATHPGVADVAV
Mb2405|M.bovis_AF2122/97 SGDLVRRRDDGNLVVTGRVKDVICRAGETIAASDLEEQLLSHPAIFSAAA
Rv2384|M.tuberculosis_H37Rv SGDLVRRRDDGNLVVTGRVKDVICRAGETIAASDLEEQLLSHPAIFSAAA
MAB_2247c|M.abscessus_ATCC_199 SGDRVRALPGGYLEVTGRIKDVILRGGESIAALDLESHLQTHPAVYAAAA
Mflv_1784|M.gilvum_PYR-GCK TGDLGRFTEDNHLVVTGRAKDIIIRNGENISPKEVEDILVTHPRIAEIAV
Mvan_4966|M.vanbaalenii_PYR-1 TGDLGHLTDDGYLVVTGRAKDLIIRNGENISPKEVEDILVTHPQIAEIAI
MSMEG_5631|M.smegmatis_MC2_155 TGDLGRWVDGDYLTVTGRAKDLIIRNGENISPKEIEDILVGELGINEIAV
MMAR_0303|M.marinum_M TGDLGHWVDDEYLVVTGRAKDIIIRNGENISPKEIEDILITHPGIADVAI
MUL_4126|M.ulcerans_Agy99 TGDIGVLDEAGNLRITDRLKDMYICGGFNVYPAEVEQVLARLDGVADVAV
MLBr_02546|M.leprae_Br4923 SGDVGYFDEHDLLYISGRDDEMIVSGGENVFPAEVEDLISGHPEVVEATA
:** : ::.* .: . .: . ::* : : :
MAV_2116|M.avium_104 IGIPDPRTGERVCAVVVPATADGVTLESLVQHCRSRGLSRYKHPEHLVVV
TH_3874|M.thermoresistible__bu IGVPDPRTGERVCAVVVPTGEQPVTLEDLAEHCRINGVARFKFPERVEFV
Mb2405|M.bovis_AF2122/97 VGLPDQYLGEKICAAVVFA-GAPITLAELNGYLDRRGVAAHTRPDQLVAM
Rv2384|M.tuberculosis_H37Rv VGLPDQYLGEKICAAVVFA-GAPITLAELNGYLDRRGVAAHTRPDQLVAM
MAB_2247c|M.abscessus_ATCC_199 VGLPDQYLGEIVCAAIVFK-GKPVAAAELNQHLQARGAATHSRVDKLVAV
Mflv_1784|M.gilvum_PYR-GCK VGIPDPRTGERACAALVTTEDPPPDIAELKDFLTDRGVARFKVPEQIHVV
Mvan_4966|M.vanbaalenii_PYR-1 VGIPDLRTGERACAVIVAADSAAPDVAEIRDFLVARGVAKFKAPERVVVV
MSMEG_5631|M.smegmatis_MC2_155 VGLPDERTGERACAVVVTEDGARPDVSALAEVLTKAGVAKFKIPEQVEIW
MMAR_0303|M.marinum_M VGLPDDRTGERACAVIVAAADPPPGLPGVRAFLEGAGVARFKIPEQVVIW
MUL_4126|M.ulcerans_Agy99 IGIPDHRLGEVGRAFIVARPGFNLDEKSVIDYTR-EHLANFKAPRSVRFV
MLBr_02546|M.leprae_Br4923 IGVDDKEWGARLRAFVVKKQDATIDEDAIKAYVR-DHLARYKVPREVIFL
:*: * * * :* : : .. :
MAV_2116|M.avium_104 DTLPRNQFGKVIKKDLRDAFG------------
TH_3874|M.thermoresistible__bu DALPRNLTGKVLKAELRKRFGG-----------
Mb2405|M.bovis_AF2122/97 PALPTTPIGKIDKRAIVRQLGIATGPVTTQRCH
Rv2384|M.tuberculosis_H37Rv PALPTTPIGKIDKRAIVRQLGIATGPVTTQRCH
MAB_2247c|M.abscessus_ATCC_199 SALPLTAVGKIDKRALLASL---TG--------
Mflv_1784|M.gilvum_PYR-GCK GALPKNDAGKVLKHVLKAELTKES---------
Mvan_4966|M.vanbaalenii_PYR-1 DALPKNDAGKVLKHAIRAVLLEKPREGEP----
MSMEG_5631|M.smegmatis_MC2_155 DALPKNDAGKVLKHQIRTQLSKVE---------
MMAR_0303|M.marinum_M DHLPKNHAGKIVKHRIKAALMKAEQ--------
MUL_4126|M.ulcerans_Agy99 DTLPRNAGGKVVKPQLRELA-------------
MLBr_02546|M.leprae_Br4923 EELPRNPTGKVLKRELRDFEVH-----------
** . **: * :