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MSAGGLHTESSPAFSDEDVARFRAAGWWCDSTLSDAVRRNAQRTPERPAYIDHGGRTFTWHEFDCAASNL AETLAGLGVGPGDRVAVWHGDCAAIHVLFVAIERRGAVIVGIGERAGAREAGAILRNAQPKILLSDQPRS KPAAEVAGELRIPALVLTQPLDTEPRAFDRDCQLGPDDVFLINSTSGTTGLPKCVVHTQNRWYYFHQKAV ANGMLTPDDVFLPVIPTPFGFGIWTSHTTPIHLGATAVILDRFTPAATCEAIARHGVTVLCCVSTQLTML MADPASRRHDLSSLRIVYTGGEALPYRPAAEFEELTGATILQFYGSNETGMLSATTVHDPRDRRLGTAGR VVPEMAVRLFDGDTDVTATGRGQPACRGPATSLGYLGGTDHDKLFTRDGWMRMGDICEIDADGYLRVTGR TSDFILRGGKNISASQVEDAAMTHPAVAIAAAVAMPDPVFGEKVCLYAELAGPATDPATDPATLDLADLT EHLLAQGFSKELLPERLVVVDELPRSSGGKVAKGRLREDIRARMEAGDERS*
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. avium 104 | MAV_2995 | - | - | 100% (542) | adenylate-forming enzyme |
| M. avium 104 | MAV_2638 | - | 1e-54 | 33.15% (534) | AMP-dependent synthetase and ligase |
| M. avium 104 | MAV_2008 | - | 4e-51 | 30.56% (540) | 2,3-dihydroxybenzoate-AMP ligase |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb2405 | mbtA | 5e-50 | 30.58% (533) | bifunctional salicyl-AMP ligase/salicyl-S-arcp synthetase |
| M. gilvum PYR-GCK | Mflv_2691 | - | 3e-48 | 31.19% (561) | AMP-dependent synthetase and ligase |
| M. tuberculosis H37Rv | Rv2384 | mbtA | 5e-50 | 30.58% (533) | bifunctional salicyl-AMP ligase/salicyl-S-arcp synthetase |
| M. leprae Br4923 | MLBr_02546 | fadD2 | 9e-35 | 26.20% (523) | acyl-CoA synthetase |
| M. abscessus ATCC 19977 | MAB_2247c | - | 7e-48 | 32.20% (528) | bifunctional salicyl-AMP ligase/salicyl-S-arcp synthetase |
| M. marinum M | MMAR_2616 | - | 0.0 | 73.02% (556) | hypothetical protein MMAR_2616 |
| M. smegmatis MC2 155 | MSMEG_4112 | - | 7e-56 | 32.18% (550) | cyclohexanecarboxylate-CoA ligase |
| M. thermoresistible (build 8) | TH_3821 | - | 5e-52 | 32.76% (467) | PUTATIVE putative adenylate-forming enzyme |
| M. ulcerans Agy99 | MUL_3145 | - | 0.0 | 75.42% (476) | hypothetical protein MUL_3145 |
| M. vanbaalenii PYR-1 | Mvan_4135 | - | 1e-48 | 30.28% (535) | AMP-dependent synthetase and ligase |
CLUSTAL 2.0.9 multiple sequence alignment
Mb2405|M.bovis_AF2122/97 -----MPPKAADGRRPSPDGGLG-GFVPFPADRAASYRAAGYWSGRTLDT
Rv2384|M.tuberculosis_H37Rv -----MPPKAADGRRPSPDGGLG-GFVPFPADRAASYRAAGYWSGRTLDT
MAB_2247c|M.abscessus_ATCC_199 ----MTQPRSTTTASSAATPALD-GFVPFPSVRADEYRRAGYWTGNTVES
Mflv_2691|M.gilvum_PYR-GCK ----MTTGFPIEYGHGLTNGDLKHGFVPFPEDRAEAYRRAGYWTGRPLDS
MMAR_2616|M.marinum_M ---------------MAVGRRDMAGSPAFSTQEAIRYHAAGWWSDSTLSD
MUL_3145|M.ulcerans_Agy99 --------------------------------------------------
MAV_2995|M.avium_104 --------------MMSAGGLHTESSPAFSDEDVARFRAAGWWCDSTLSD
TH_3821|M.thermoresistible__bu -------------------------MLEADPDAVALIRGDQRWSR-----
MSMEG_4112|M.smegmatis_MC2_155 ---------MTAPTERRLANVLNGQYTPIDDDTAANWRAAGWWENRSIRS
Mvan_4135|M.vanbaalenii_PYR-1 -------------------------MRRIPAQLVERYQREGYWTQETLGD
MLBr_02546|M.leprae_Br4923 MTKLRHYLERGAAEVHYLRSIFESGALGFEPPLTYAAMAVAMRKWGDLAM
Mb2405|M.bovis_AF2122/97 VLSDAARRWPDRLAVADAGDRPGHGGLSYAELDQRADRAAAALHGLGITP
Rv2384|M.tuberculosis_H37Rv VLSDAARRWPDRLAVADAGDRPGHGGLSYAELDQRADRAAAALHGLGITP
MAB_2247c|M.abscessus_ATCC_199 LLRVSAARRPCHPAVIDE-----HRALSYRELDEAADNAAHAFLRLGIAP
Mflv_2691|M.gilvum_PYR-GCK ILTDAASRWPDKPAIIDA-----TRSYTFAELDAAADRAAAGLADLGIRP
MMAR_2616|M.marinum_M AVRRNAECSPHHAAYIDYP----ASLLTWSEFDCAATNLAEQLAGAGVLP
MUL_3145|M.ulcerans_Agy99 -----------------------------------------------MLP
MAV_2995|M.avium_104 AVRRNAQRTPERPAYIDHG----GRTFTWHEFDCAASNLAETLAGLGVGP
TH_3821|M.thermoresistible__bu -----------------------------EQLWELSGRAVAKLRSLGAEP
MSMEG_4112|M.smegmatis_MC2_155 LLADAAQAHPDRIALVGRRADGGRVARTYQEFDRNANQVASVLASLGVRP
Mvan_4135|M.vanbaalenii_PYR-1 MLARGLAGSPEAGFCVHSSIR--PYRGTFGEVERDARRLAAGLRERGVGP
MLBr_02546|M.leprae_Br4923 LPAMPARRSPHRAALIDEE-----GTLTYKELDRAAHALANGLIAKGVRG
Mb2405|M.bovis_AF2122/97 GDRVLLQLPNGCQFAVALFALLRAGAIPVMCLPGHRAAELGHFAAVSAAT
Rv2384|M.tuberculosis_H37Rv GDRVLLQLPNGCQFAVALFALLRAGAIPVMCLPGHRAAELGHFAAVSAAT
MAB_2247c|M.abscessus_ATCC_199 GDRVLLQLPNRASFAVALFGLMRAGAIPVMCLPGHRAAELSHFIEVADAV
Mflv_2691|M.gilvum_PYR-GCK GERVLLQLPNSCQFAVALFALLRAGVVPVMCLTGHRTAELGHFADVSGAV
MMAR_2616|M.marinum_M GDRVAVWHGDCAALHALFIAIERCGAVVVGIGARAGIREATQILRAAQVK
MUL_3145|M.ulcerans_Agy99 GDRVAVWHGDCAALHALFIAIERCGAVVVGIGARAGVREATQILRAAQVK
MAV_2995|M.avium_104 GDRVAVWHGDCAAIHVLFVAIERRGAVIVGIGERAGAREAGAILRNAQPK
TH_3821|M.thermoresistible__bu GDAVVAHYDTGAEEMAIALAASRLGCVFMPIPRRLGARELNYVVGLADPV
MSMEG_4112|M.smegmatis_MC2_155 DDAVVVMLPNWVEYPEFLFGINELGAIYAGIPVAYGDQQAAAILRRSRAR
Mvan_4135|M.vanbaalenii_PYR-1 GDVVAMQLPNWREAAVTFWASAFLGAVVVPIVHFYGRRELAHIMATAKPR
MLBr_02546|M.leprae_Br4923 GDGIAILARNHRWFVIANYGCARVGARIILLNSEFSGPQIKEVSEREGAK
.: : . * : .
Mb2405|M.bovis_AF2122/97 GLVVADVASGFDYRPMARELVADHPTLRHVIVDGDPGPFVSWAQLCAQAG
Rv2384|M.tuberculosis_H37Rv GLVVADVASGFDYRPMARELVADHPTLRHVIVDGDPGPFVSWAQLCAQAG
MAB_2247c|M.abscessus_ATCC_199 GLVIPDSAGGFDYRALAVALSQRHPRLRTVVVEGDAGPFASWTSLLKSVE
Mflv_2691|M.gilvum_PYR-GCK AVIVPDRAGGFDYRDMAASLAAQRPALRHVIVDGEPGDQVSWQSILDFAG
MMAR_2616|M.marinum_M LLVSDAPRSAAATEVSAQLPVPSGVLVREGKTLRFDGKPVPVAPENGLES
MUL_3145|M.ulcerans_Agy99 LLVSDAPRSAAATEVSAQLPVPSGVLVREGKTLRFDGKPVPVAPENGLES
MAV_2995|M.avium_104 ILLSDQPRSKPAAEVAGELRIPALVLT----------QPLDTEPR-----
TH_3821|M.thermoresistible__bu LVAVQDRDHVGVVENPGRARVLT------------------LADSLAPTG
MSMEG_4112|M.smegmatis_MC2_155 VLVIPRRWRGNNILEQSRRLRDQIPTLQQVIVLDDDGTDLRDGESLWSDH
Mvan_4135|M.vanbaalenii_PYR-1 VFVTAEEFGRMRYQP---DLCGDVAVVGLVGAAHPAAGNEHAFEQLLADE
MLBr_02546|M.leprae_Br4923 VIIYDDEYTKVVSKAEPALGKLRALGVNPDEEEPPGSTDETLAELIARSS
.
Mb2405|M.bovis_AF2122/97 TGSPA---PPADPGSPALLLVSGGTTGMPKLIPRTHDDYVFNATASAALC
Rv2384|M.tuberculosis_H37Rv TGSPA---PPADPGSPALLLVSGGTTGMPKLIPRTHDDYVFNATASAALC
MAB_2247c|M.abscessus_ATCC_199 PQGPL---PTVQTDTPALLLVSGGTTAAPKLIPRTHEDYVYNATQAARVC
Mflv_2691|M.gilvum_PYR-GCK PRLGR---TPVDPSLPALLLVSGGTTGLPKLIARTHDDYVYNAHACAQAC
MMAR_2616|M.marinum_M ELGNE---RRLGPDDVFLINSTSGTTGAPKCVVHTQNRWHYFHQHAVANG
MUL_3145|M.ulcerans_Agy99 ELGNE---RRLGPDDVFLINSTSGTTGAPKCVVHTQNRWHYFHQHAVANG
MAV_2995|M.avium_104 AFDRD---CQLGPDDVFLINSTSGTTGLPKCVVHTQNRWYYFHQKAVANG
TH_3821|M.thermoresistible__bu AEVDP---YRWRPGEAALVGFTSGSTGHPKGVMHGVPAMEWVADLIVERG
MSMEG_4112|M.smegmatis_MC2_155 AHVAARQFPPPDPGQICYLGFTSGTTGEPKGAMHSHNTLIYSARRQAEHI
Mvan_4135|M.vanbaalenii_PYR-1 PMAGT---VAADPAAPTLIAFTSGTTRDPKGVIHSHQTLSCETRQLLENY
MLBr_02546|M.leprae_Br4923 SAPAP-----KVRKRASIIILTSGTTGTPKGANRNTPASLAPLGGILSKV
: :.*:* ** :
Mb2405|M.bovis_AF2122/97 ---RLSADDVYLVVLAAGHNFPLACPGLLGAMTVGATAVFAPDPSPEAAF
Rv2384|M.tuberculosis_H37Rv ---RLSADDVYLVVLAAGHNFPLACPGLLGAMTVGATAVFAPDPSPEAAF
MAB_2247c|M.abscessus_ATCC_199 ---GLTQDDVYLVALPAGHNFPLACPGLLGAISTGATTVFLSDPSPESAF
Mflv_2691|M.gilvum_PYR-GCK ---EMTGEDAYLVALPAGHNFPLACPGLLGSMTVGAPTVFTADPSPENAF
MMAR_2616|M.marinum_M ---MLTPQDVVLPVIPMPFGFGIWTS-HTTPIYLGASAVILDRFTTLAAC
MUL_3145|M.ulcerans_Agy99 ---MLTPQDVVLPVIPMPFGFGIWTS-HTTPIYLGASAVILDRFTTLAAC
MAV_2995|M.avium_104 ---MLTPDDVFLPVIPTPFGFGIWTS-HTTPIHLGATAVILDRFTPAATC
TH_3821|M.thermoresistible__bu ---SLRPGEP-ICVTGAGAGAPGFTFYTYLGLTRGLPIVKSEKWDPKRVL
MSMEG_4112|M.smegmatis_MC2_155 GTEAFGEPVVNLVASPMGHHTGYVWGGVFTVMLAG-TAVHVDRWDPTWGA
Mvan_4135|M.vanbaalenii_PYR-1 P----PDRGRQLTATPVGH-FIGMLGAFLIPVLEGAPIDLCDVWDPGRVL
MLBr_02546|M.leprae_Br4923 P---FRAHEVTLLPAPMFHALGYLHA--TLAMFLGSTLVLRRRFKPATVL
: : * . .
Mb2405|M.bovis_AF2122/97 AAIERHGVTVTALVPALAKLWAQSC--EWEPVTPKSLRLLQVGGSKLEPE
Rv2384|M.tuberculosis_H37Rv AAIERHGVTVTALVPALAKLWAQSC--EWEPVTPKSLRLLQVGGSKLEPE
MAB_2247c|M.abscessus_ATCC_199 ATIARHRVSVTAVVPSLAQLWAQAT--AWEPVLPETLRLLQVGGAKLGAD
Mflv_2691|M.gilvum_PYR-GCK ALIDKHRITVTGLVNALAKLWAQAC--DWEPVLPTSLRFVQVGGSRMSPE
MMAR_2616|M.marinum_M EAIARHRVTVLCCVSTQLTMLMADH--WCRAYDLSSLRVVFAGGEALPYR
MUL_3145|M.ulcerans_Agy99 EAIARHRVTVLCCVSTQLTMLMADH--WCRAYDLSSLRVVFAGGEALPYR
MAV_2995|M.avium_104 EAIARHGVTVLCCVSTQLTMLMADP--ASRRHDLSSLRIVYTGGEALPYR
TH_3821|M.thermoresistible__bu ELMARERCVWSTMVPTMLHMLMTAQRDSGRAYDLSAMRTVSMGGAPMSEE
MSMEG_4112|M.smegmatis_MC2_155 QVVREEGVTTFFGAPTFLQDIIRTE---LAGDPACPLRCMVVAGAPVPRN
Mvan_4135|M.vanbaalenii_PYR-1 KLIESDGLSIGGGPPYFVTSLLDHP--DFRPEHLRRFKTVGLGGSTVP-A
MLBr_02546|M.leprae_Br4923 EDIEKHQATAMVVVPVMLSRILDTLENLETKSDLSSLRVVFVSGSQLGAE
: . :: : .* :
Mb2405|M.bovis_AF2122/97 DARRVRTALTPGLQQVFG-MAEGLLNFTRIGDPPEVVEHTQGRPLCPADE
Rv2384|M.tuberculosis_H37Rv DARRVRTALTPGLQQVFG-MAEGLLNFTRIGDPPEVVEHTQGRPLCPADE
MAB_2247c|M.abscessus_ATCC_199 DARAVRESLTQGLQQVFG-MAEGLLCLTQPGDPTDILDNSQGRPMCDADE
Mflv_2691|M.gilvum_PYR-GCK EARFILDRLTPGMSQIFG-MAEGMLNFTRPGDPEDVVVHTQGRPMSPHDE
MMAR_2616|M.marinum_M RATEFEDLTGAAILQFYGSNETGLLSATTLRDSRERRLRTGGR-VVPEMS
MUL_3145|M.ulcerans_Agy99 RATEFEDLTGAAILQFYGSNETGLLSATTLRDSRERRLRTGGR-VVPEMS
MAV_2995|M.avium_104 PAAEFEELTGATILQFYGSNETGMLSATTVHDPRDRRLGTAGR-VVPEMA
TH_3821|M.thermoresistible__bu FISEARELMTFEPLRVYAMAECMMHSHSRADDPIEIRNGMDGR-PGPGAE
MSMEG_4112|M.smegmatis_MC2_155 LPAQAAEALGAYVAPAWGMTECSILTSCTPDEP-DAILRTDGS-VFAGSE
Mvan_4135|M.vanbaalenii_PYR-1 AVTQRLTDLGLFVFRSYGSTEHPSITGSRPDAPEVKRLFTDGN-------
MLBr_02546|M.leprae_Br4923 LATRALEELGPVIYNMYGSTEIAFATIAGPKDLQINSATVGPV--VKGVK
:.
Mb2405|M.bovis_AF2122/97 LRIVNADGEPVGPGEEGELLVRGPYTLNGYFAAERDNERCFDPDGFYRSG
Rv2384|M.tuberculosis_H37Rv LRIVNADGEPVGPGEEGELLVRGPYTLNGYFAAERDNERCFDPDGFYRSG
MAB_2247c|M.abscessus_ATCC_199 VRIVDEDGADVPAGETGELLVRGPYTLNGYYRADADNMRSFTVDGFYRSG
Mflv_2691|M.gilvum_PYR-GCK MRVVDEDGVEVAPGEEGELLVRGPYTLNGYYRADDANARSFTPDGFYRTG
MMAR_2616|M.marinum_M VRLFDGD-RDVTESGRGQPACRGPATSLGYLGG-TDHEKLFTRDGWMRMG
MUL_3145|M.ulcerans_Agy99 VRLFDGD-RDVTASGRGQPGCRGPATSLGYLGG-TDHEKLFTRDGWMRMG
MAV_2995|M.avium_104 VRLFDGD-TDVTATGRGQPACRGPATSLGYLGG-TDHDKLFTRDGWMRMG
TH_3821|M.thermoresistible__bu IATYDSEGRRLPTGETGEIGMRGPSLMLGYLGD-APGSLPLTSDGFFLSG
MSMEG_4112|M.smegmatis_MC2_155 VKIVDDTGAAVAAGVVGDLLMRGPGVVYGYYDRPDATRDAYLPGLWFKTG
Mvan_4135|M.vanbaalenii_PYR-1 ----VRPGVEIRLGEDGEIFSRGPDLCLGYTDD-ELTARAFDDDGWYRTG
MLBr_02546|M.leprae_Br4923 VKILDDSGKEVPRGQVGRIFVGNAFPFEGYTGG----GGKQIIDGLLSSG
: * .. ** . *
Mb2405|M.bovis_AF2122/97 DLVRRRDDG----NLVVTGRVKDVICRAGETIAASDLEEQLLSHPAIFSA
Rv2384|M.tuberculosis_H37Rv DLVRRRDDG----NLVVTGRVKDVICRAGETIAASDLEEQLLSHPAIFSA
MAB_2247c|M.abscessus_ATCC_199 DRVRALPGG----YLEVTGRIKDVILRGGESIAALDLESHLQTHPAVYAA
Mflv_2691|M.gilvum_PYR-GCK DRVRIFTDGPRAGYVEVTGRVKDVIHRGGETVSATDLEDHLHTHPSVYAA
MMAR_2616|M.marinum_M DICEIDPDG----YLTVTGRTSDFILRGGKNISAGQVEDAALTHPAVAIA
MUL_3145|M.ulcerans_Agy99 DICEIDPDG----YLTVTGRTSDFILRGGKNISAGQVEDAALTHPAVAIA
MAV_2995|M.avium_104 DICEIDADG----YLRVTGRTSDFILRGGKNISASQVEDAAMTHPAVAIA
TH_3821|M.thermoresistible__bu DLGRVEDNG----CIKVVGRLKDMIIRGGYNIDPGEVEELLRAHPEVRDV
MSMEG_4112|M.smegmatis_MC2_155 DRADVDENG----WLRLRGRSKDIIIRGGENIPVTDVESAIFDHPDVLNA
Mvan_4135|M.vanbaalenii_PYR-1 DVGVLDDDG----YLTITDRKADVIIRGGENISALEVEEVLLAMPAVAEA
MLBr_02546|M.leprae_Br4923 DVGYFDEHD----LLYISGRDDEMIVSGGENVFPAEVEDLISGHPEVVEA
* . : : .* :.* .* .: ::*. * : .
Mb2405|M.bovis_AF2122/97 AAVGLPDQYLGEKICAAVVFAG---------APITLAELNGYLDRRGVAA
Rv2384|M.tuberculosis_H37Rv AAVGLPDQYLGEKICAAVVFAG---------APITLAELNGYLDRRGVAA
MAB_2247c|M.abscessus_ATCC_199 AAVGLPDQYLGEIVCAAIVFKG---------KPVAAAELNQHLQARGAAT
Mflv_2691|M.gilvum_PYR-GCK AAVALPDEYLGEKICAAVVFTG---------PPLTLADLNAFLDERGAST
MMAR_2616|M.marinum_M AAVSMPDPVFGEKVCLYVELA--------DSQRLDRTELVEHLLALGYSK
MUL_3145|M.ulcerans_Agy99 AAVSMPDPVFGEKVCLYVELA--------DSQRLDRTELVEHLLALGYSK
MAV_2995|M.avium_104 AAVAMPDPVFGEKVCLYAELAGPATDPATDPATLDLADLTEHLLAQGFSK
TH_3821|M.thermoresistible__bu AIVGYPDPVYGERACAVLWTDG---------VVLGLSDIAEFLLERNLSK
MSMEG_4112|M.smegmatis_MC2_155 AVIGLPDERLGERVCAVLVTKSG-------CPELTVDTLGEYLLGQGLSK
Mvan_4135|M.vanbaalenii_PYR-1 VVVAAPDARLGEHTAAVLRIREG-------QSMPALGDVREHFKQAGVAT
MLBr_02546|M.leprae_Br4923 TAIGVDDKEWGARLRAFVVKKQD--------ATIDEDAIKAYVR-DHLAR
. :. * * : .. :
Mb2405|M.bovis_AF2122/97 HTRPDQLVAMP---ALPTTPIGKIDKRAIVRQLGIATGPVTTQRCH
Rv2384|M.tuberculosis_H37Rv HTRPDQLVAMP---ALPTTPIGKIDKRAIVRQLGIATGPVTTQRCH
MAB_2247c|M.abscessus_ATCC_199 HSRVDKLVAVS---ALPLTAVGKIDKRALLASL---TG--------
Mflv_2691|M.gilvum_PYR-GCK HARPDVLVPVP---SLPTTAVGKVDKKALVAQLTANS---------
MMAR_2616|M.marinum_M ELLPERLVVVD---ELPRSSGGKIAKGQLRDDIRARMEAEHERS--
MUL_3145|M.ulcerans_Agy99 ELLPERLVVVD---ELPRSSGGKIAKGQLRDDIRARMEAEHERS--
MAV_2995|M.avium_104 ELLPERLVVVD---ELPRSSGGKVAKGRLREDIRARMEAGDERS--
TH_3821|M.thermoresistible__bu DKIPEMVVCRD---ELPQSPDGKILKGQLRAEIAALVATRSG----
MSMEG_4112|M.smegmatis_MC2_155 HYLPEKVVHLD---ELPMTPSGKIQKFKLREQYS------------
Mvan_4135|M.vanbaalenii_PYR-1 QKWPEELHRVPEGQDFPRTASGKVQKFVIRQQLAAGELSAIATGQE
MLBr_02546|M.leprae_Br4923 YKVPREVIFLE---ELPRNPTGKVLKRELRDFEVH-----------
: :* .. **: * :