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TTLETAPTLDEFDVSKIVRSDRVHGSVYTSPEIFRREMDTIFKTGWVYVAHESEVSERGDYLTRMIGYDP VVVVRGKDGVVRVVLNRCTHRANKLCNAEKGNANSFRCPYHGWTFNNTGALQGVPMREGYGEAFNEIRAE LGLAQAPRVDSYGGFIFASLAAEGVSLREHLGNATHAIDRLLNLSPNRQIDLRANWMKHLHHANWKMVVE NNVDGYHALFTHASVYDAIKPAKVSHVPTKVDVLVRDLGNGHSEIDYSDEYRKLDEEFVWYGRIPRAKLA KYVDALEKAYGPEQAHDALVVGPPHTLIWPNLFLAEMNVMFVEPQAPDRTIAYTTAVLIPGQDELNERTL RRSEGAMGPAGFLIADDGEIGMRNQAGLAAELPEWLVLARGTENDITDDSGIINRDKSAETPQRGFYAQW AAVVGGGVRA*
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. avium 104 | MAV_1901 | - | - | 100% (431) | dioxygenase large subunit |
| M. avium 104 | MAV_2612 | - | 0.0 | 89.93% (427) | dioxygenase large subunit |
| M. avium 104 | MAV_5084 | - | e-103 | 43.77% (409) | dioxygenase large subunit |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb3186c | - | 6e-21 | 29.13% (206) | dioxygenase |
| M. gilvum PYR-GCK | Mflv_1355 | - | 3e-49 | 30.71% (420) | Rieske (2Fe-2S) domain-containing protein |
| M. tuberculosis H37Rv | Rv3161c | - | 3e-21 | 29.61% (206) | dioxygenase |
| M. leprae Br4923 | - | - | - | - | - |
| M. abscessus ATCC 19977 | MAB_4370c | - | 7e-37 | 27.78% (432) | putative large terminal subunit of phenylpropionate dioxygenase |
| M. marinum M | MMAR_0307 | - | 1e-19 | 30.20% (202) | dioxygenase |
| M. smegmatis MC2 155 | MSMEG_1908 | - | 2e-45 | 28.64% (419) | benzoate 1,2-dioxygenase alpha subunit |
| M. thermoresistible (build 8) | TH_2976 | - | 1e-103 | 45.05% (404) | PUTATIVE dioxygenase large subunit |
| M. ulcerans Agy99 | MUL_3820 | - | 9e-14 | 26.81% (235) | ring-hydroxylating dioxygenase, large terminal subunit |
| M. vanbaalenii PYR-1 | Mvan_0488 | - | 3e-40 | 35.85% (265) | ring hydroxylating dioxygenase, alpha subunit |
CLUSTAL 2.0.9 multiple sequence alignment
Mb3186c|M.bovis_AF2122/97 --MLSTDNRAELGDILTDIGDYLDDNPP-------ALSLPPAAYTSSELW
Rv3161c|M.tuberculosis_H37Rv --MLSTDNRAELGDILTDIGDYLDDNPP-------ALSLPPAAYTSSELW
MAV_1901|M.avium_104 ----MTTLETAPTLDEFDVSKIVRSD-----------RVHGSVYTSPEIF
TH_2976|M.thermoresistible__bu ---------------------LIRPD-----------RVHGSLYTDPAIF
Mflv_1355|M.gilvum_PYR-GCK MTQTTASPQPRTHLAAVLADAVIDDPEAG------VFRANRRIFTDEEIF
MSMEG_1908|M.smegmatis_MC2_155 -MTTHLSP---DHLENVLADAVIEDPAAG------VYRANRRIFTDDEIF
MAB_4370c|M.abscessus_ATCC_199 -MASLPRPVAVAQGALCRPQALVRDIDVSGLVDSHGGLLDRVIFTDGELY
Mvan_0488|M.vanbaalenii_PYR-1 -MTTETTGTADATDPYLR--RALREVADG----LKVGRLPARVVSDPALH
MUL_3820|M.ulcerans_Agy99 --MDRDQLIDLTRRALKLARDKTTDLAPH------QHAVDAREYTSLDRH
MMAR_0307|M.marinum_M -MTDHNDGRAEPMDAAHHDDVEATLTG--------AVTFGVDAYTCADYA
:
Mb3186c|M.bovis_AF2122/97 QLERERIFNRSWMLVAHVDQLAKTGDYVTVSVAGEPVMVVRDVDGQLHAL
Rv3161c|M.tuberculosis_H37Rv QLERERIFNRSWMLVAHVDQVAKTGDYVTVSVAGEPVMVVRDVDGQLHAL
MAV_1901|M.avium_104 RREMDTIFKTGWVYVAHESEVSERGDYLTRMIGYDPVVVVRGKDGVVRVV
TH_2976|M.thermoresistible__bu AEELRKIWYRTWVFVGHESEVSQPNDYVRKKLGLQDVIMTRDRDGEIHLL
Mflv_1355|M.gilvum_PYR-GCK ELEMKHIFEGNWIYLAHESQVANPGDYFTTYIGRQPVVITRSKDGSLNCL
MSMEG_1908|M.smegmatis_MC2_155 ELEMKHIFEGNWVYLAHESQVPNPGDYFTTYIGRQPVVITRDRDGDLHCL
MAB_4370c|M.abscessus_ATCC_199 RQELRRVFAPSWLFLAHADQFHRPGDFFTTYMGEDPVIVSMDKNRRIRAF
Mvan_0488|M.vanbaalenii_PYR-1 TIEMERIFGRAWVFLGHESELAKSGDFVVRHIGADSVIVCRDNSGRIQAL
MUL_3820|M.ulcerans_Agy99 ERDRAMVLD-SPQLVGYSSELPGPDTYRTKTIMGRSILLTRAADRAVRAF
MMAR_0307|M.marinum_M RAERDKLWRKVWQQVGRVEDLPKVGSYLTYDILDDSIVVVRSAADTFKAH
: : :. .:. . : : ::: ..
Mb3186c|M.bovis_AF2122/97 SPICRHRLMLMVEPG------AGRIDTLTCQYHLWRYGLDGRLRGAPHMA
Rv3161c|M.tuberculosis_H37Rv SPICRHRLMLMVEPG------AGRIDTLTCQYHLWRYGLDGRLRGAPHMA
MAV_1901|M.avium_104 LNRCTHRANKLCNAE------KGNANSFRCPYHGWTFNNTGALQGVPMR-
TH_2976|M.thermoresistible__bu LNRCSHRGNQVCADA------KGNSSTFRCPYHGWTFRNTGELVGFPFF-
Mflv_1355|M.gilvum_PYR-GCK INACAHRGAMICRRK------TDNRMTLTCPFHGWTFRNDGALLKVKDP-
MSMEG_1908|M.smegmatis_MC2_155 INACAHRGAMICRRK------TDNRTTLTCPFHGWTFRNDGTLLKVKDP-
MAB_4370c|M.abscessus_ATCC_199 LNSCRHRGARVCRAD------YGVTRGFTCTYHGWAYDLEGKLISVPNE-
Mvan_0488|M.vanbaalenii_PYR-1 SNSCRHRGALVCRAE------MGNTAHFQCPYHGWVYSNTGELVGVPAMS
MUL_3820|M.ulcerans_Agy99 DNVCLHRQSRVATG-------CGTALRFTCPYHGWTYDNGGRLVGLPGR-
MMAR_0307|M.marinum_M HNVCMHRGRRLVDIPPGAKNVCGHKKSFVCGFHGWTYDQDGNCIHIPEKP
* ** : . : * :* * : *
Mb3186c|M.bovis_AF2122/97 ANLDFN-----RRECRLPQFA-VATWNGLVWINLDADA-EPIAAHLDLTD
Rv3161c|M.tuberculosis_H37Rv ANLDFN-----RRECRLPQFA-VATWNGLVWINLDADA-EPIAAHLDLTD
MAV_1901|M.avium_104 EGYGEAFNE-IRAELGLAQAPRVDSYGGFIFASLAAEG-VSLREHLGNAT
TH_2976|M.thermoresistible__bu KGYGTR-----KLDLPLGRVPRVDSYGGFVFGSFATEG-PTLREHLGAAA
Mflv_1355|M.gilvum_PYR-GCK DGAGYPEAFNVNGSHNMTTVARFESYRGFLFGSLNPDV-LPLTEHLGDAT
MSMEG_1908|M.smegmatis_MC2_155 DGAGYPDTFNSNGSHNMTNVARFEGYRGFLFGSLNPDV-LPLTEHLGDTT
MAB_4370c|M.abscessus_ATCC_199 EGYPDSFS---TDDWGLVEVPHVDSYHGLIFGTWNPAP-VGLRESLGDMA
Mvan_0488|M.vanbaalenii_PYR-1 EAYPGGFD---KSQWGLRHIPHVDSYAGFIFGSVDPKA-PSLTDYLGDTT
MUL_3820|M.ulcerans_Agy99 EGFPGIAVG----SDGLTELP-ATEFAGFLWVSLHPAARLDVPAHLGPLA
MMAR_0307|M.marinum_M DWQGALTP-----ENTHLAAVNVDTWGGWIWINMDPAA-QPLRQYLEPAA
. : * :: . . : *
Mb3186c|M.bovis_AF2122/97 DEFAGYRLG-----EMVQVESWSHEWRANWKVAAEN-GHENYHVLGLHRQ
Rv3161c|M.tuberculosis_H37Rv DEFAGYRLG-----EMVQVESWSHEWRANWKVAAEN-GHENYHVLGLHRQ
MAV_1901|M.avium_104 HAIDRLLNLSPNRQIDLRANWMKHLHHANWKMVVEN-NVDGYHALFTHAS
TH_2976|M.thermoresistible__bu GEIDRLLRLSPEGKVALDAGWLRHRTRANWKLLVEN-ETDGYHPQFVHGG
Mflv_1355|M.gilvum_PYR-GCK KIIDMLVDQSPDG-LEVLRGSSTYTYDGNWKVQAEN-GADGYHVTATHWN
MSMEG_1908|M.smegmatis_MC2_155 TVIDMLVDQSPDG-LEVLRGASTYTYDGNWKVQAEN-GADGYHVTATHWN
MAB_4370c|M.abscessus_ATCC_199 WYMDAMLDHDSEG-TVVVGGVHKWVLDGNWKLAAEQFATDWYHVNMSHAS
Mvan_0488|M.vanbaalenii_PYR-1 FYLDLIAKKTAGG-LEVIGAPHRWVMSANWKTAADNFVGDSYHTLFAHR-
MUL_3820|M.ulcerans_Agy99 GELQSWGIG-----RWSPLGEKVLGSPIDWKLATDT-FAENYHFATVHRR
MMAR_0307|M.marinum_M TMLDPFRLQD-----MRCKWRKWLTFQCNWKVAMEA-FNETYHVATTHPQ
: :** : : ** *
Mb3186c|M.bovis_AF2122/97 T-----LEPFVP--------------------GGGDLDVRQYSRWALRLR
Rv3161c|M.tuberculosis_H37Rv T-----LEPFVP--------------------GGGDLDVRQYSRWALRLR
MAV_1901|M.avium_104 V-----YDAIKP-AKVSHVPTKVDVLVRDLGNGHSEIDYSDEYRKLDEEF
TH_2976|M.thermoresistible__bu I-----FGVTGS-TIGPLYSDSSTAVTRDLGGGHSENDLRPEFRKFAEPL
Mflv_1355|M.gilvum_PYR-GCK ------YAATTS-RRGTGESKNETKALDAGGWGKSGGGYWSFPHGHLCLW
MSMEG_1908|M.smegmatis_MC2_155 ------YAATTS-RRTCGESKNDTKALDAGSWGKSGGGYWSFPHGHLCLW
MAB_4370c|M.abscessus_ATCC_199 A-----LMVMAPQGKAPKDEIVHRTGRQYVDPNGHGAGFPVHPKNRFDAR
Mvan_0488|M.vanbaalenii_PYR-1 --------SMVELGMAPGDPNFASAPAEISLQNGHGVGVLGFPPTLADFP
MUL_3820|M.ulcerans_Agy99 T-----FATIAR-------------------SNCTVFDSYGPHHRLIFPL
MMAR_0307|M.marinum_M FNKFGNFRGWAAAHGRHSNIGYDAPKDLAQTKSKIRLGTGADPRVSTAEM
. .
Mb3186c|M.bovis_AF2122/97 VPFTVPVEAKSLQLNEVQKSNLV---------------------------
Rv3161c|M.tuberculosis_H37Rv VPFTVPVEAKSLQLNEVQKSNLV---------------------------
MAV_1901|M.avium_104 VWYGRIPRAKLAKYVDALEKAYGPEQAHDALVV--------------GPP
TH_2976|M.thermoresistible__bu RWFG-TTESRVPDYVAAMRARYG-AEADQILIE--------------GSP
Mflv_1355|M.gilvum_PYR-GCK TWAANPQDRPLWDKLDELKATHG-DAKGEFMVK--------------GSR
MSMEG_1908|M.smegmatis_MC2_155 TWAANPQDRPLWDRLDELKARFG-DAKGEFMVK--------------GSR
MAB_4370c|M.abscessus_ATCC_199 VVHQWTDYGALRERLG-EARVQGPLTNG----------------------
Mvan_0488|M.vanbaalenii_PYR-1 EYEGYPDEVVDQMATSYPSPVHKDLMRRSSFI------------------
MUL_3820|M.ulcerans_Agy99 NTILELENVPEGQWDPFHHMVVI---------------------------
MMAR_0307|M.marinum_M QRYTLEETNATTTRTLVDAALRLVEELPEDAPAGQVLDHWLTSARRDDAA
Mb3186c|M.bovis_AF2122/97 VLWTFPNSALAIAGER----------VVWFGFIPQSIDRVQVLGGVLT--
Rv3161c|M.tuberculosis_H37Rv VLWTFPNSALAIAGER----------VVWFGFIPQSIDRVQVLGGVLT--
MAV_1901|M.avium_104 HTLIWPNLFLAEMN--------------VMFVEPQAPDRTIAYTTAVLIP
TH_2976|M.thermoresistible__bu HVMIFPNLFIAEIQ--------------VFNIQPVAVNECVQYSTAVQLV
Mflv_1355|M.gilvum_PYR-GCK NLCVYPNVYVMDQFST-----------QIRHFRPIAPDKTEVTIYCIAPK
MSMEG_1908|M.smegmatis_MC2_155 NLCLYPNVYVMDQFST-----------QIRHFRPIAPDKTEVTIYCIAPK
MAB_4370c|M.abscessus_ATCC_199 HATVFPNLSYLPVNG------------SVRIWHPKGPDKMEVWAWTIVDR
Mvan_0488|M.vanbaalenii_PYR-1 HGTVFPNLSFINVTLAQDHMSPPTPFITFRVWHPLSHDRMEVLSWFLVER
MUL_3820|M.ulcerans_Agy99 -YALFPNVVLSVTIAN-------------GELFRIYPGDRPGSSITVHQN
MMAR_0307|M.marinum_M RGVLWPTIDPEHLAQSG---------TAWQIFPNFQIGQGLTTALCYSAR
:*. .
Mb3186c|M.bovis_AF2122/97 --TPELAADAAATAQTSQFVMAMINDEDRLGLEAVQVGAGSRFAERGHLS
Rv3161c|M.tuberculosis_H37Rv --TPELAADAAATAQTSQFVMAMINDEDRLGLEAVQVGAGSRFAERGHLS
MAV_1901|M.avium_104 GQ-DELNERTLRRSEGAMGPAGFLIADDGEIGMRNQAGLAAELPEWLVLA
TH_2976|M.thermoresistible__bu GA-PELNRRMVSQCIGSVGPAGMLLADDTEMYERNQQGLEALTPEWLDIR
Mflv_1355|M.gilvum_PYR-GCK SESAQARANRIRQYEDFFNASGMATPDDLEEFRSCQLTFRAEAAPWNDMS
MSMEG_1908|M.smegmatis_MC2_155 GESAAARAHRIRQYEDFFNASGMATPDDLEEFRSCQLTFRAQAAPWNDMS
MAB_4370c|M.abscessus_ATCC_199 SMPEEVRDAQRLYNLRTFGPTGIFEQDDGENWSEVQSISRGFVTNSVALN
Mvan_0488|M.vanbaalenii_PYR-1 DAPEWLRDASQASYVNNFGPGGVFEQDDAEAWKAITESVQGPFAGEGLLN
MUL_3820|M.ulcerans_Agy99 STPLDLSDESVATGAQAVFDYAHATVRDEDYRLAETLQTNLESGARQRLV
MMAR_0307|M.marinum_M PHGYDPDQCVFEASCYELYPKGEEPHTEWVYAPPDDPGWRSVLPQDFSNM
. :
Mb3186c|M.bovis_AF2122/97 -------------------------------SKEWPGMLAFYRNLAMALV
Rv3161c|M.tuberculosis_H37Rv -------------------------------SKEWPGMLAFYRNLAMALV
MAV_1901|M.avium_104 RGTENDITD--------------DSGIINRDKSAETPQRGFYAQWAAVVG
TH_2976|M.thermoresistible__bu RGLNREHVD--------------DHGFTVGGPTDETGMRAFWSEYRRLME
Mflv_1355|M.gilvum_PYR-GCK RGAQHWLDGPDDVARSLGLDAVISAGVKNEDEGLYPVQHGYWLETMRAAA
MSMEG_1908|M.smegmatis_MC2_155 RGAQHWLSGPDPVAESLGMHGIVSSGLRNEDEGLYPVQHGYWLETLRRAV
MAB_4370c|M.abscessus_ATCC_199 YQMGLDSE-REDGR---------HPG-LTSELYSDAAGRSFYARWRDLMN
Mvan_0488|M.vanbaalenii_PYR-1 YEMGMDLTPLTDWP---------GPGEALPSGYAEQNQRRFWGRWLEYMG
MUL_3820|M.ulcerans_Agy99 FG------------------------------RNEPGLQHRHITWAQALE
MMAR_0307|M.marinum_M AAVQQGMKSG-------------GFGGPKPNPYMERSTANLHRNLAEYMG
Mb3186c|M.bovis_AF2122/97 GDHPGAS--
Rv3161c|M.tuberculosis_H37Rv GDHPGAS--
MAV_1901|M.avium_104 GGVRA----
TH_2976|M.thermoresistible__bu AE-------
Mflv_1355|M.gilvum_PYR-GCK QKEAR----
MSMEG_1908|M.smegmatis_MC2_155 TSNPTEH--
MAB_4370c|M.abscessus_ATCC_199 TKAWHEEHV
Mvan_0488|M.vanbaalenii_PYR-1 QPPAFGGRA
MUL_3820|M.ulcerans_Agy99 TPG------
MMAR_0307|M.marinum_M TGAPREL--