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LIGDIATNNARRYPQKRALVDADRVHTWTQVDERARRLAGYLAGRGLEPGERVMVIARNCIEWPEISFGL AKAGLIAVPVNIRLAPDEVAHVRDDCGARGVIVHADHLDKFSSEIDRLDVVLPIGDEYESALAAAEPVAP QVAISPDDVAVILYTSGTTGRAKGVMHTHRGLLYQAADTNLVTEANRSDVMLATTPFFTAGGMVRTVSWL YLGQTMVIHQRFDPQAVIDEIERSSITFTTFIPTMLHRTLAILEDGPPRDMSSLRRISYGSAPVPPGLAR KAMDLLGCDLQQRYGLTECGGQATILTPQDHRDIVAGRTSIATSCGRETPMCVIRVIDAEGNDAPVDEVG EIVIVSPANAVGYWNQPEQTAETFRPDGLRTGDLGYLDADGYLHITGRRTDLIISGGFNVYPAEIERVIA QHPGVDMVAVVGVPDPEWGETPVAAVIPKSHVRDQQALTEELAALCRAELAGYKQPRRFEYRDEFPLGPA GKILKREIANQVRHVGSGA*
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. avium 104 | MAV_1899 | - | - | 100% (510) | feruloyl-CoA synthetase |
| M. avium 104 | MAV_2610 | - | 0.0 | 85.30% (517) | acyl-CoA synthase |
| M. avium 104 | MAV_3523 | - | 1e-79 | 36.38% (503) | acyl-CoA synthase |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb0172 | fadD5 | 7e-72 | 35.84% (505) | acyl-CoA synthetase |
| M. gilvum PYR-GCK | Mflv_0027 | - | 1e-93 | 38.15% (519) | AMP-dependent synthetase and ligase |
| M. tuberculosis H37Rv | Rv0166 | fadD5 | 7e-72 | 36.04% (505) | acyl-CoA synthetase |
| M. leprae Br4923 | MLBr_02546 | fadD2 | 4e-55 | 30.68% (502) | acyl-CoA synthetase |
| M. abscessus ATCC 19977 | MAB_4559 | - | 2e-70 | 32.74% (507) | putative long-chain-fatty-acid CoA ligase |
| M. marinum M | MMAR_0409 | fadD5 | 5e-71 | 34.85% (505) | fatty-acid-CoA ligase FadD5 |
| M. smegmatis MC2 155 | MSMEG_0131 | - | 2e-72 | 36.54% (509) | acyl-CoA synthetase |
| M. thermoresistible (build 8) | TH_2854 | - | 4e-80 | 37.40% (508) | PUTATIVE putative AMP-dependent synthetase and ligase |
| M. ulcerans Agy99 | MUL_4126 | fadD3 | 2e-57 | 33.14% (510) | acyl-CoA synthetase |
| M. vanbaalenii PYR-1 | Mvan_0943 | - | 1e-87 | 37.45% (518) | AMP-dependent synthetase and ligase |
CLUSTAL 2.0.9 multiple sequence alignment
MAV_1899|M.avium_104 ---------------------------------------------MLIGD
TH_2854|M.thermoresistible__bu ----------------------------------------VSRSHLLIGD
Mb0172|M.bovis_AF2122/97 --------------------MTAQLASHLTRALTLAQQQPYLARRQNWVN
Rv0166|M.tuberculosis_H37Rv --------------------MTAQLASHLTRALTLAQQQPYLARRQNWVN
MMAR_0409|M.marinum_M --------------------MTAELASHLPQAA-HALEQPYLARRQNWVN
MSMEG_0131|M.smegmatis_MC2_155 --------------------MTAEPS---------QIEQPYLARRQNWVN
MAB_4559|M.abscessus_ATCC_1997 ---------------------------------------------MQFTQ
Mflv_0027|M.gilvum_PYR-GCK -------------------------------------------MFHNLAD
Mvan_0943|M.vanbaalenii_PYR-1 -------------------------------------------MIHNLAD
MUL_4126|M.ulcerans_Agy99 ----------------------------------------MTSDPRTVPT
MLBr_02546|M.leprae_Br4923 MTKLRHYLERGAAEVHYLRSIFESGALGFEPPLTYAAMAVAMRKWGDLAM
MAV_1899|M.avium_104 IATNNARRYPQKRALVDADRVHTWTQVDERARRLAGYLAGRGLEPGERVM
TH_2854|M.thermoresistible__bu LITRAARTYGAAPALVHGDRVMSFREFDDATNRLGNALLARGLRPGDHVA
Mb0172|M.bovis_AF2122/97 QLERHAMMQPDAPALRFVGNTMTWADLRRRVAALAGALSGRGVGFGDRVM
Rv0166|M.tuberculosis_H37Rv QLERHAMMQPDAPALRFVGNTMTWADLRRRVAALAGALSGRGVGFGDRVM
MMAR_0409|M.marinum_M QLERHAMMQPRATAIRYLGHTVTWADLRYRVAALAGSLSRRGVGFGDRVM
MSMEG_0131|M.smegmatis_MC2_155 QLTRHAMMQPDKTALRFLGRTTTWRELDDRVTRLAGALNRRGVGFGDRVL
MAB_4559|M.abscessus_ATCC_1997 WLHRALQQYPDRLITIDQGREHTVEQFADRVARLSAGLRANGVRPGDRVG
Mflv_0027|M.gilvum_PYR-GCK IVRSHGRNRPDAPALIVGDRTVSYRELDERSNRTAQAFSRTGVGPGDRVA
Mvan_0943|M.vanbaalenii_PYR-1 IIRVHGRERPGAPALVVGDRTVTYGELDDRSSRAAQAFAQAGVGVGDRVA
MUL_4126|M.ulcerans_Agy99 ALDRLVRRLPNHDALVTDERSFTVSELNDEVYRAAAALIELGVKPAARVG
MLBr_02546|M.leprae_Br4923 LPAMPARRSPHRAALIDEEGTLTYKELDRAAHALANGLIAKGVRGGDGIA
: :. . : *: . :
MAV_1899|M.avium_104 VIARNCIEWPEISFGLAKAGLIAVPVNIRLAPDEVAHVRDDCGARGVIVH
TH_2854|M.thermoresistible__bu VLAPNGIDGVVTYYALAKAGLVRVPLNSRETDFELAYKIDDSQARALVTD
Mb0172|M.bovis_AF2122/97 ILMLNRTEFVESVLAANMIGAIAVPLNFRLTPTEIAVLVEDCAAHVMLTE
Rv0166|M.tuberculosis_H37Rv ILMLNRTEFVESVLAANMIGAIAVPLNFRLTPTEIAVLVEDCVAHVMLTE
MMAR_0409|M.marinum_M ILMLNRTEFVESVLAANMLGAIAVPLNFRLTPSEIAFLVEDCAPRLVITE
MSMEG_0131|M.smegmatis_MC2_155 ILMLNRTEFVEAMLAANRLGAIAVPVNFRMTPPEIAYLVSDCEAHVVVTE
MAB_4559|M.abscessus_ATCC_1997 MLSLNSDRYIEYLTAVPWLGAALNAVNIRWSLAEIGYSLRESGTRVLLVD
Mflv_0027|M.gilvum_PYR-GCK FVERNGIEFFDVAFGSAKLGAVAVPVNWRLTAPEMRHVIADCGASVVVVG
Mvan_0943|M.vanbaalenii_PYR-1 FVDKNGAEFFEVTFGLAKVGAVGVPVNWRLAAPEMRHIIADSGAKIVVVG
MUL_4126|M.ulcerans_Agy99 IWSPNTWHWVVACLAIHHVGAAMVPLNTRYTADEAADILARTEAPVLFAM
MLBr_02546|M.leprae_Br4923 ILARNHRWFVIANYGCARVGARIILLNSEFSGPQIKEVSEREGAKVIIYD
. * . * :* . : : . :.
MAV_1899|M.avium_104 ADHLD------KFSSEIDRLDVVLPIG--------DEYESALAAAEPVAP
TH_2854|M.thermoresistible__bu G----------RIPAET---ELVIHT---------EELAGMIAGADPGPC
Mb0172|M.bovis_AF2122/97 AALAP---VAIGVRNIQPLLSVIVVAG-GSSQDSVFGYEDLLNEAGDVHE
Rv0166|M.tuberculosis_H37Rv AALAP---VAIGVRNIQPLLSVIVVAG-GSSQDSVFGYEDLLNEAGDVHE
MMAR_0409|M.marinum_M EVLAQ---VAVGVREIAPALSTIVVAG-GASDPTVVGYDELISETGDPPE
MSMEG_0131|M.smegmatis_MC2_155 PVLAG---VATAVRDLDATLQTVIVAG-GATEDGVLGYDDLIAEEGEPPA
MAB_4559|M.abscessus_ATCC_1997 DTFKA---AAAPLRAACACLETVIYCGAGPAPQDMIGYEDLLAS-HEPID
Mflv_0027|M.gilvum_PYR-GCK QEFTG---RIEAIADD--LDVEVVVIG---KHRHWPTFDDLLAAAPAVDP
Mvan_0943|M.vanbaalenii_PYR-1 QDFAG---HLEAIEDG--LDADIVVIG---DHPRWPAFDDWVASHPPVDP
MUL_4126|M.ulcerans_Agy99 GHFLGSDRVAGLDRKALPALRHIVRIPIEEPDGTWDEFIETGTDTGAVTA
MLBr_02546|M.leprae_Br4923 DEYTK---VVSKAEPALGKLRALGVNP-DEEEPPGSTDETLAELIARSSS
:
MAV_1899|M.avium_104 -QVAISPDDVAVILYTSGTTGRAKGVMHTHRGLLYQAAD--TNLVTEANR
TH_2854|M.thermoresistible__bu -RIERDPEDPLRLAYTGGTTGKPKAVVLTTRSELAEIANFLVDLLPDLTP
Mb0172|M.bovis_AF2122/97 -PVDIPNDSPALIMYTAGTTGRPKGAVLTHANLTGQAMTALYTSGANINS
Rv0166|M.tuberculosis_H37Rv -PVDIPNDSPALIMYTSGTTGRPKGAVLTHANLTGQAMTALYTSGANINS
MMAR_0409|M.marinum_M -PVDIPNDSPALIMYTSGTTGRPKGAVLTHTNLTGQVMTALYTGGANINS
MSMEG_0131|M.smegmatis_MC2_155 -PVDIPNDSPALIMYTSGTTGRPKGAVLTHTNLSAQAMMHLFTTGADLNS
MAB_4559|M.abscessus_ATCC_1997 -DTRTGGDSLLGVFYTGGTTGNPKGVMLSHNNVLASAMGSLSTGNFLTRG
Mflv_0027|M.gilvum_PYR-GCK -GVVTGPDDLVFLMYTSGTTGAPKGVMLSNANVVCKTAGVGGPWKFDADS
Mvan_0943|M.vanbaalenii_PYR-1 -GVVTGPDDVVLLMYTSGTTGAPKGVMLSNTNYVYKTGGVAGPWQFDADA
MUL_4126|M.ulcerans_Agy99 RAAAVSPDDVSDILFTSGTTGRSKGVLCAHR-QSLSASASWAANGKITAD
MLBr_02546|M.leprae_Br4923 APAPKVRKRASIIILTSGTTGTPKGANRNTPASLAPLGGILSKVPFRAHE
. : *.**** .*..
MAV_1899|M.avium_104 SDVMLATTPFFTAGGMVRTVSWLYLGQTMVIHQR--FDPQAVIDEIERSS
TH_2854|M.thermoresistible__bu DSVMLHAAP-ITHGSGAFFLPHLVKGARNVILPR--FTPQGFVDAAVEHG
Mb0172|M.bovis_AF2122/97 DVGFVGVPLFHIAGIG-NMLTGLLLGLPTVIYPLGAFDPGQLLDVLEAEK
Rv0166|M.tuberculosis_H37Rv DVGFVGVPLFHIAGIG-NMLTGLLLGLPTVIYPLGAFDPGQLLDVLEAEK
MMAR_0409|M.marinum_M DVGFIGVPFFHIAGIG-NMLSGMLLGVPTVIYPLGAFNPGQLLDVLEAER
MSMEG_0131|M.smegmatis_MC2_155 DVGFVGVPLFHIAGVAGSVLSGVTLGVPTVLYPLGAFDPDGLLDVLESEQ
MAB_4559|M.abscessus_ATCC_1997 GRLLHSAPMFHMADFS-AVLAGNLSGSTHVIVPS--FSPQGVLDAIVAHD
Mflv_0027|M.gilvum_PYR-GCK -VSLAVMPLFHMAGFG-WALAGLWEGAVTVVLRD--VDPGAILDAVARHR
Mvan_0943|M.vanbaalenii_PYR-1 -VSLAVMPLFHMAGSG-WALAGLWQGATTVVLRD--VEPAAILDAIARHR
MUL_4126|M.ulcerans_Agy99 DRHLCINPFFHNFGYKAGILACLQTGAT--LFPHLTFDPLRTLQAIERHR
MLBr_02546|M.leprae_Br4923 VTLLP-APMFHALGYLHATLA-MFLGSTLVLRRR--FKPATVLEDIEKHQ
: . . :. * : . * ::
MAV_1899|M.avium_104 ITFTTFIPTMLHRTLAILEDGPP-RDMSSLRRISYGSAPVPPGLARKAMD
TH_2854|M.thermoresistible__bu ATATFMVPTMIAMMLEDAEID---RDRLKLRRLCYGGAPIAPTLLARGLE
Mb0172|M.bovis_AF2122/97 VTGIFLVPAQWQAVCTEQQARP--RDLR-LRVLSWGAAPAPDALLRQMSA
Rv0166|M.tuberculosis_H37Rv VTGIFLVPAQWQAVCTEQQARP--RDLR-LRVLSWGAAPAPDALLRQMSA
MMAR_0409|M.marinum_M VSGIFLVPAQWQAVCAEQQARP--RELS-LRVMSWGAAPAPDALLRQMSE
MSMEG_0131|M.smegmatis_MC2_155 VTGIFLVPAQWQAVCAAQRARP--RNLK-LRALSWGAAPASDTLLREMNE
MAB_4559|M.abscessus_ATCC_1997 VQDMLLVPTMIQMLVDHPGAAQ--LDLSGIRSITYGASVISEAVLQRATR
Mflv_0027|M.gilvum_PYR-GCK VTNMLLVPAVIQFLLDTTGLDD--TDLSRLRIVVYGASPITDDVLMRGID
Mvan_0943|M.vanbaalenii_PYR-1 ITNMLLVPAVIQFLLDTDGVAE--VDLSTLRVIVYGASPISDDVLVRGIE
MUL_4126|M.ulcerans_Agy99 ITVLPGPPTIYQTLLDHPARDD--YDLSSLRFAVTGAATVPVVLVERMQS
MLBr_02546|M.leprae_Br4923 ATAMVVVPVMLSRILDTLENLETKSDLSSLRVVFVSGSQLGAELATRALE
*. : :* ..: : .
MAV_1899|M.avium_104 LLG-CDLQQRYGLTECGGQATILTPQDHRDIVAGRTSIATSCGRETPMCV
TH_2854|M.thermoresistible__bu TLG-PVFVQLYGQAEAPLAITCLQPWEH------TPERLTSAGKPYTFVE
Mb0172|M.bovis_AF2122/97 TFPETQILAAFGQTEMSPVTCMLLGEDAIA-------KRGSVGRVIPTVA
Rv0166|M.tuberculosis_H37Rv TFPETQILAAFGQTEMSPVTCMLLGEDAIA-------KRGSVGRVIPTVA
MMAR_0409|M.marinum_M VFPGTQIMAAFGQTEMSPVTCMLLGEDAIR-------KRGSVGKVIPTVS
MSMEG_0131|M.smegmatis_MC2_155 VFPDAQIFAAFGQTEMSPVTCMLLGDDAIR-------KLGSVGRVIPTVS
MAB_4559|M.abscessus_ATCC_1997 VFPNARFTQAYGMTEVSPVATLLLPDDHDDP-----ALLRSAGRAAPHCE
Mflv_0027|M.gilvum_PYR-GCK RFG-GIFAQVYGMTESTGSVTQLDGDEHLP------QLLRSCGRPYPWVQ
Mvan_0943|M.vanbaalenii_PYR-1 RFG-PIFAQVYGMTETTGSITQLDGPDHVP------ALLRSCGRPYPWVQ
MUL_4126|M.ulcerans_Agy99 ELDIDIVLTAYGLTEANGMGTMCRADDDAL------TVATTCGRPFADFE
MLBr_02546|M.leprae_Br4923 ELG-PVIYNMYGSTEIAFATIAGPKDLQIN--------SATVGPVVKGVK
: . :* :* : *
MAV_1899|M.avium_104 IRVIDAEGNDAPVDEVGEIVIVSPANAVGYWNQPEQTAETFRPDG-LRTG
TH_2854|M.thermoresistible__bu MDIRDEEDNSVPPGTPGEVVTRGPHTMSGYWRRPEATAETLGEDGWLRTG
Mb0172|M.bovis_AF2122/97 ARVVDQNMNDVPVGEVGEIVYRAPTLMSCYWNNPEATAEAFA-GGWFHSG
Rv0166|M.tuberculosis_H37Rv ARVVDQNMNDVPVGEVGEIVYRAPTLMSCYWNNPEATAEAFA-GGWFHSG
MMAR_0409|M.marinum_M ARVVDDEMNDVPIGQVGEIVYRAPTLMSGYWNNPDATAEAFA-GGWFHSG
MSMEG_0131|M.smegmatis_MC2_155 ARVVDDNMNDVPVGEVGEIVYRGPNLMVGYWNNPKATAEAFA-GGWFHSG
MAB_4559|M.abscessus_ATCC_1997 VRIVDPDDNEVPRGEIGEVIVKGDNVMLGYWELPEESAAAIR-DGWMHTG
Mflv_0027|M.gilvum_PYR-GCK IRVVDPTGRDVAPGTVGEVWTRSDQNMLGYWNNPEATAATLTPDGWLRTG
Mvan_0943|M.vanbaalenii_PYR-1 IRIVDETGADAVAGTVGEVWTRSEQNMLGYWNNPDATASTLTADGWLKTG
MUL_4126|M.ulcerans_Agy99 LRIG---------PDTGEVLLRGPNVMLGYLDDPRATAAAIDADGWLHTG
MLBr_02546|M.leprae_Br4923 VKILDDSGKEVPRGQVGRIFVGNAFPFEGYTGG----GGKQIIDGLLSSG
: *.: * . .* : :*
MAV_1899|M.avium_104 DLGYLDADGYLHITGRRTDLIISGGFNVYPAEIERVIAQHPGVDMVAVVG
TH_2854|M.thermoresistible__bu DIGQVDEEGYLYLLDRRNDVIISGGFNVYPREVEDALLTHPAVREAVVVG
Mb0172|M.bovis_AF2122/97 DLVRMDSDGYVWVVDRKKDMIISGGENIYCAELENVLASHPDIAEVAVIG
Rv0166|M.tuberculosis_H37Rv DLVRMDSDGYVWVVDRKKDMIISGGENIYCAELENVLASHPDIAEVAVIG
MMAR_0409|M.marinum_M DLVRMDEDGYVWVVDRKKDMIISGGENVYCAEVENVLASHPSIVEVAVIG
MSMEG_0131|M.smegmatis_MC2_155 DLVRQDEEGYVWVVDRKKDMIISGGENIYCAEVENVLAAHPAIMEVAVIG
MAB_4559|M.abscessus_ATCC_1997 DAGRMDDRGYVYIVDRIKDMVVTGGENVYSAEVENALAKHPAVAACAVIG
Mflv_0027|M.gilvum_PYR-GCK DAGYLDRDGYLYLHDRIKDMIVSGAENVYPAEVENVLMTHPAVTDVAVIG
Mvan_0943|M.vanbaalenii_PYR-1 DAGYVDDDGYLFLHDRIKDMIVSGGENVYPVEVENVLMTHPAVADAAVIG
MUL_4126|M.ulcerans_Agy99 DIGVLDEAGNLRITDRLKDMYICGGFNVYPAEVEQVLARLDGVADVAVIG
MLBr_02546|M.leprae_Br4923 DVGYFDEHDLLYISGRDDEMIVSGGENVFPAEVEDLISGHPEVVEATAIG
* * . : : .* :: : *. *:: *:* : : ..:*
MAV_1899|M.avium_104 VPDPEWGETPVAAVIPKSHVRDQQALTEELAALCRAELAGYKQPRRFEYR
TH_2854|M.thermoresistible__bu VDDERWGQRVSAAVVLREEVD-----TQALIDHCAERLAGFKRPRHIEVW
Mb0172|M.bovis_AF2122/97 RADEKWGEVPIAVAAV---TN-DDLRIEDLGEFLTDRLARYKHPKALEIV
Rv0166|M.tuberculosis_H37Rv RADEKWGEVPIAVAAV---TN-DDLRIEDLGEFLTDRLARYKHPKALEIV
MMAR_0409|M.marinum_M RADEKWGEVPIAVAAV---TK-DHLRIEDLDEYLTERLARYKHPKALEIV
MSMEG_0131|M.smegmatis_MC2_155 RPHEKWGEVPVAVVALNSATA-ESISLGELDSFLTERLARYKHPKALEVV
MAB_4559|M.abscessus_ATCC_1997 IPDEQWGERVHAVVVK--QAQ-SECCDGDLQEHCREHIANYKVPRSFEFV
Mflv_0027|M.gilvum_PYR-GCK IPDTRWGEAVKAVVVAG-----APVTEAELIAFAREQLAGFKLPKSVDFV
Mvan_0943|M.vanbaalenii_PYR-1 VPDRRWGEAVKAVVVAAR--G-AQLTEAELIAFARDRIGGFKLPKSVDFV
MUL_4126|M.ulcerans_Agy99 IPDHRLGEVGRAFIVAR--PG-FNLDEKSVIDYTREHLANFKAPRSVRFV
MLBr_02546|M.leprae_Br4923 VDDKEWGARLRAFVVKK---QDATIDEDAIKAYVRDHLARYKVPREVIFL
. . * * : .:. :* *: .
MAV_1899|M.avium_104 DEFPLGPAGKILKREIANQVRH---VGSGA---------------
TH_2854|M.thermoresistible__bu DELPKSPVGKSLRRTVRETMRANEEVTAHA---------------
Mb0172|M.bovis_AF2122/97 DALPRNPAGKVLKTELRLRYGACVNVERRSASAGFTERRENRQKL
Rv0166|M.tuberculosis_H37Rv DALPRNPAGKVLKTELRLRYGACVNVERRSASAGFTERRENRQKL
MMAR_0409|M.marinum_M DALPRNPSGKVLKTELRLRYGVGVYSESRSVSRSFEFREGS----
MSMEG_0131|M.smegmatis_MC2_155 DALPRNPSGKVLKTELRERFGRATSLDGGESNSATTVSAG-----
MAB_4559|M.abscessus_ATCC_1997 DELPLSGAGKILKRVLRQKY-----WESRDAGVS-----------
Mflv_0027|M.gilvum_PYR-GCK DALPRNPSGKLLKRQLREPY-----WTDEDRHIG-----------
Mvan_0943|M.vanbaalenii_PYR-1 DVLPRNPSGKLLKRALREPY-----WDGADRHIG-----------
MUL_4126|M.ulcerans_Agy99 DTLPRNAGGKVVKPQLRELA-------------------------
MLBr_02546|M.leprae_Br4923 EELPRNPTGKVLKRELRDFEVH-----------------------
: :* . ** :: :