For questions or suggestions e-mail us at: ioerger@cs.tamu.edu
MSASAVTSPVEGRVTDTPDAEYVRGMARLLQAVQELSLARSLPEIQRIVRTAARELTGCDGATFVLRDNG KCYYADEDAIAPLWKGSRFPMEICISGWAMLNREAAIIPDIYLDGRIPHEAYRPTFVKSLVMAPIRKLDP VGAIGNYWAQERQPTPREVSLLQALADATSIAMENVQIYAELEQRVRDRTAAVEQANEEIRQLSLTDDLT GLNNRRGFRLLAEAALLAARTHGHDCALAFLDVDGLKRVNDGIGHDAGDSLIVDVARVLRETLGPSAIVA RLGGDEFCTLVPEYSDGAAALTARVQDAFRRFDARSSRPYQVMISVGVVEIPPTDVSPLEDLLSRADELM YAQKKAKSCAEIA
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. gilvum PYR-GCK | Mflv_4515 | - | - | 100% (363) | diguanylate cyclase |
| M. gilvum PYR-GCK | Mflv_4377 | - | 7e-20 | 33.19% (232) | diguanylate cyclase/phosphodiesterase with GAF sensor |
| M. gilvum PYR-GCK | Mflv_2883 | - | 4e-18 | 36.14% (166) | diguanylate cyclase with PAS/PAC sensor |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb1389c | - | 4e-18 | 35.36% (181) | hypothetical protein Mb1389c |
| M. tuberculosis H37Rv | Rv1354c | - | 4e-18 | 35.36% (181) | hypothetical protein Rv1354c |
| M. leprae Br4923 | MLBr_00397 | - | 1e-19 | 37.11% (159) | putative transporter protein |
| M. abscessus ATCC 19977 | MAB_0124 | - | 5e-15 | 36.31% (168) | hypothetical protein MAB_0124 |
| M. marinum M | - | - | - | - | - |
| M. avium 104 | MAV_4962 | - | 1e-135 | 66.67% (360) | sensory box histidine kinase |
| M. smegmatis MC2 155 | MSMEG_2196 | - | 9e-19 | 30.50% (259) | sensory box/response regulator |
| M. thermoresistible (build 8) | TH_0811 | - | 2e-20 | 36.53% (219) | sensory box/response regulator |
| M. ulcerans Agy99 | - | - | - | - | - |
| M. vanbaalenii PYR-1 | Mvan_1967 | - | 8e-20 | 33.89% (239) | diguanylate cyclase/phosphodiesterase with GAF sensor |
CLUSTAL 2.0.9 multiple sequence alignment
Mflv_4515|M.gilvum_PYR-GCK MSASAVTSPVEGRVTDTPDAEYVRGMARLLQAVQELSLARSLPEIQRIVR
MAV_4962|M.avium_104 MTAEQVSQPPASGSSRFAVYDYVDGMERLVHAVQELSLARSLPDIQRIVR
MSMEG_2196|M.smegmatis_MC2_155 ----------------MSES-----LDVLVTSVATQLMAVEAATSVSVSQ
Mvan_1967|M.vanbaalenii_PYR-1 ----------------MSDSDTLRSLDLVITSVATKLMAANAATSVEVSQ
TH_0811|M.thermoresistible__bu ----------------VSTE-----LDLLVTGVATKLMAADAATARDVSR
Mb1389c|M.bovis_AF2122/97 ----------------MCNDTATPQLEELVTTVANQLMTVDAATSAEVSQ
Rv1354c|M.tuberculosis_H37Rv ----------------MCNDTATPQLEELVTTVANQLMTVDAATSAEVSQ
MLBr_00397|M.leprae_Br4923 -------------MDHTTKCDAEQYFQAIVTSMADGVIVVDIDGRIESIN
MAB_0124|M.abscessus_ATCC_1997 -----------MGAGEMTFGQEYRFATRALTVTRNLVPLKIAIGLLCLSI
:
Mflv_4515|M.gilvum_PYR-GCK TAARELTGCDGAT----FVLRDNGKCYYADEDAIAPLWKGSRFPMEICIS
MAV_4962|M.avium_104 SSARELTGCDGAT----FVLRDNDRCYYADEDAIAPLWKGSRFPMTSCIS
MSMEG_2196|M.smegmatis_MC2_155 QVLAELVSFFDVD----VSFLRHNDHRAHATRLVAEWPPRPVDAATDPIA
Mvan_1967|M.vanbaalenii_PYR-1 RVLAELVDYLGVD----VSFLRYNDHKIRASRLVAEWPVRPGVPDPDPLA
TH_0811|M.thermoresistible__bu RVLADLVRAFELD----FSFLRHNDHEIRASKLIAEWPVRENVPDPDPIG
Mb1389c|M.bovis_AF2122/97 RVLAYLVEQLGVD----VSFLRHNDRDRRATRLVAEWPPRLNIPDPDPLR
Rv1354c|M.tuberculosis_H37Rv RVLAYLVEQLGVD----VSFLRHNDRDRRATRLVAEWPPRLNIPDPDPLR
MLBr_00397|M.leprae_Br4923 PAATRILGLRAHD----VVDMKHG-------------------------H
MAB_0124|M.abscessus_ATCC_1997 VVLGTLIQFHPLGPHGLWPRLIHGVLVASALVVGMCWVVLPWPRRRVAIA
: .
Mflv_4515|M.gilvum_PYR-GCK GWAMLNREAAIIPDIYLDGRIPHEAYRPTFVKSLVMAPIRKLDPVGAIGN
MAV_4962|M.avium_104 GWAMLNRDAAVIPDIYRDPRIPHNLYRPTFVKSLVMVPIRKLDPIGAIGN
MSMEG_2196|M.smegmatis_MC2_155 VVHFADADPVFAMAEHLK---EPAVFRPEPLYDDYQRTIEAGRHIPAT-S
Mvan_1967|M.vanbaalenii_PYR-1 LVYFADADPVFAQSEHGK---KPMVFRPEPATDDYQKRIEETRAVPAT-S
TH_0811|M.thermoresistible__bu VVHFEGADPVFALAEHAK---EIHIFRPEPATDEFQRLIEESSGVPAS-T
Mb1389c|M.bovis_AF2122/97 LIYFADADPVFALCEHAK---EPLVFRPEPATEDYQRLIEEARGVPVT-S
Rv1354c|M.tuberculosis_H37Rv LIYFADADPVFALCEHAK---EPLVFRPEPATEDYQRLIEEARGVPVT-S
MLBr_00397|M.leprae_Br4923 PFCFYDTD-------------NQRVDLEREVMRVVRREVTTVSKVVGIDQ
MAB_0124|M.abscessus_ATCC_1997 FVWWADISMVVGACTLSAPASRLGALAHMGLIGVFAAFLLGWRVLAVHCV
. . : :
Mflv_4515|M.gilvum_PYR-GCK YWAQERQPTPREVSLLQALADATSIAMENVQIYAELEQRVRDRTAAV--E
MAV_4962|M.avium_104 YWAEPHQPSEHEVRLLQALADSTSMAMENVAIYSEMEQRVRDRTAAL--E
MSMEG_2196|M.smegmatis_MC2_155 MACVPLLSGDVTTGVLGFVKFGDREWLPAELNALKAIASLFAQVQAR--I
Mvan_1967|M.vanbaalenii_PYR-1 MAAAPLVSGELTTGVLGFIKFGDREWTAEELNGLEAIASLFAQVQAR--V
TH_0811|M.thermoresistible__bu VAAVPLVSGEITTGVLGFVKYGDRQWHPDELNALKAIASLFAQVQAR--V
Mb1389c|M.bovis_AF2122/97 AAAVPLVSGEITTGLLGFIKFGDRKWHEAELNALMTIATLFAQVQAR--V
Rv1354c|M.tuberculosis_H37Rv AAAVPLVSGEITTGLLGFIKFGDRKWHEAELNALMTIATLFAQVQAR--V
MLBr_00397|M.leprae_Br4923 HSGQRLWLS-VNVSLLAYKAPPHS-----------ALVVSFSDISAH--H
MAB_0124|M.abscessus_ATCC_1997 FATVAIFGLMGWNVRLGEATWFDQYVYSAPALSSVVLLPIIIQVVVEGGR
* .
Mflv_4515|M.gilvum_PYR-GCK QANEEIRQLSLTDDLTGLNNRRGFRLLAEAALLAARTHGHDCALAFLDVD
MAV_4962|M.avium_104 RANEEIRRLSVTDELTGLNNRRGFYLLAGQKLSGSHRLGHACVLAFLDVD
MSMEG_2196|M.smegmatis_MC2_155 EAEERLRYLADHDHLTGLYNRRALMAHLEARLAPGQPG--PVAVMFFDLD
Mvan_1967|M.vanbaalenii_PYR-1 QAEEQLRYLAEHDDLTGLYNRRALLAHLDERLAEGRPG--PVSALFFDLD
TH_0811|M.thermoresistible__bu EAEERLRYLAEHDDLTGLHNRRALLAHLDARLAAGQPG--PVSALYFDLD
Mb1389c|M.bovis_AF2122/97 AAEARLRYLADHDDLTGLHNRRALLQHLDQRLAPGQPG--PVAALFLDLD
Rv1354c|M.tuberculosis_H37Rv AAEARLRYLADHDDLTGLHNRRALLQHLDQRLAPGQPG--PVAALFLDLD
MLBr_00397|M.leprae_Br4923 LSIERLTYEATHDCLTGLANRRFAEDQITKSLQHDERS-RLAAVLLLDLD
MAB_0124|M.abscessus_ATCC_1997 RSLKAISLAAHQDPLTGLLNRRGVQVALSSLLS-GQRATRIVAVVLVDVD
: : : * **** *** * . .*:*
Mflv_4515|M.gilvum_PYR-GCK GLKRVNDGIGHDAGDSLIVDVARVLRETLGPSAIVARLGGDEFCTLVPE-
MAV_4962|M.avium_104 GLKQVNDEQGHDAGDMLIKDVAEVLRSIRRESDILARIGGDEFCVMVTE-
MSMEG_2196|M.smegmatis_MC2_155 RLKAINDYLGHTAGDAFISILAQRLQRGDDAPKLIARLGGDEFVVVPDDP
Mvan_1967|M.vanbaalenii_PYR-1 RLKAINDYLGHTAGDWFIRVLAERLRRGADCPNIIARLGGDEFVLVPTVP
TH_0811|M.thermoresistible__bu RLKAVNDYLGHTVGDWFIRVLAERLREGAGPDDLIARLGGDEFVVVPSGP
Mb1389c|M.bovis_AF2122/97 RLKAINDYLGHAAGDQFIHVFAQRIGDALVGESLIARLGGDEFVLIPASP
Rv1354c|M.tuberculosis_H37Rv RLKAINDYLGHAAGDQFIHVFAQRIGDALVGESLIARLGGDEFVLIPASP
MLBr_00397|M.leprae_Br4923 DFKVINDSLGHDVGDAVLQTVAQRLRSAVRPDDVVARLGGDEFIVLLRGP
MAB_0124|M.abscessus_ATCC_1997 ELKELNDRLGHDAGDESIRAVAAMLATTTRASDLTARMGGDEFMVVGFFD
:* :** ** .** : .* : : **:***** :
Mflv_4515|M.gilvum_PYR-GCK -YSDGAAALTARVQDAFRRFDARSSRPYQVMISVGVVEIPPTDVSPLEDL
MAV_4962|M.avium_104 -SDQDSAPLRERLAEAFAAFNATGDRPYRLSASVGLVRAPVFDTASVDEL
MSMEG_2196|M.smegmatis_MC2_155 MSLDEATALAYRLQSVLRERVTVDGEMLTRTVSIGIAQG-IPGKDSTSDV
Mvan_1967|M.vanbaalenii_PYR-1 MDGAAAEALAHRLQSTLRERVSIDGEMLTRTVSIGVAQG-IPGRDTTSDL
TH_0811|M.thermoresistible__bu VDADAALELANRLRSRLQQQVCVDGEMLTRTASIGVAQG-TPGRDTTSDL
Mb1389c|M.bovis_AF2122/97 MSADAAQPLAERLRDQLKDHVAIGGEVLTRTVSIGVASG-TPGQHTPSDL
Rv1354c|M.tuberculosis_H37Rv MSADAAQPLAERLRDQLKDHVAIGGEVLTRTVSIGVASG-TPGQHTPSDL
MLBr_00397|M.leprae_Br4923 LSDMNANDVAKRLHTTLSESLVVDQLTVPIGASVGILEVRPDDRRRAADI
MAB_0124|M.abscessus_ATCC_1997 HADEATGLIERIGARRFGIGPHRASVSMGSAMRSTSVAG-----FDFESL
: : : .:
Mflv_4515|M.gilvum_PYR-GCK LSRADELMYAQKKAK-----SCAEIA------------------------
MAV_4962|M.avium_104 LARADELMYVEKKAR-----RTAG--------------------------
MSMEG_2196|M.smegmatis_MC2_155 LNRADHAVLTAKGSG-----GNRVAVFSDAMAMEIDFRNDIELHLQSVIE
Mvan_1967|M.vanbaalenii_PYR-1 LRRADQAVLTAKGSG-----GNEVVVFTDDMSMRHEFNNDIELHLQSVIE
TH_0811|M.thermoresistible__bu LRRADQAVLTAKSAG-----GNQITVFTDDMSLEAEFRNDIELHLQSVIQ
Mb1389c|M.bovis_AF2122/97 LRRADQAALAAKHAG-----GDSVAIFTADMSVSGELRNDIELHLRRGIE
Rv1354c|M.tuberculosis_H37Rv LRRADQAALAAKHAG-----GDSVAIFTADMSVSGELRNDIELHLRRGIE
MLBr_00397|M.leprae_Br4923 LRDADSAMYAAKNKKQCAVTPQQLVPFVALIALFVFFTAAAGAKFYAPSN
MAB_0124|M.abscessus_ATCC_1997 RRAADEELRGAKIER-----RNGFHAVP----------------------
** *
Mflv_4515|M.gilvum_PYR-GCK --------------------------------------------------
MAV_4962|M.avium_104 --------------------------------------------------
MSMEG_2196|M.smegmatis_MC2_155 GGALVLHYLPEIDMRTGEVLAAEALVRWEHPTRGLLSPDSFIGVAESINL
Mvan_1967|M.vanbaalenii_PYR-1 NGALLLHYLPEVDMRTGEILACEALVRWQHPTRGLLSPESFIGVAESINL
TH_0811|M.thermoresistible__bu DEALLLHFLPEVDMRTGEILATEALVRWHHPTRGLLAPDTFIGVAESVNL
Mb1389c|M.bovis_AF2122/97 SDALRLVYLPEVDLRTGDIVGTEALVRWQHPTRGLLAPGCFIPVAESINL
Rv1354c|M.tuberculosis_H37Rv SDALRLVYLPEVDLRTGDIVGTEALVRWQHPTRGLLAPGCFIPVAESINL
MLBr_00397|M.leprae_Br4923 LLVILQQTVVLAIVGYGMTFVIMAGSVDLSVGSIVALTGVTAALVAAQNQ
MAB_0124|M.abscessus_ATCC_1997 --------------------------------------------------
Mflv_4515|M.gilvum_PYR-GCK --------------------------------------------------
MAV_4962|M.avium_104 --------------------------------------------------
MSMEG_2196|M.smegmatis_MC2_155 AGELGRWVLRTACAEFSRWRANGVGRNIVLRINVSPVQLVTDGFVESVAG
Mvan_1967|M.vanbaalenii_PYR-1 AGELGRWVMRSACADFAGWRARGIGTGTVMRINVSPVQLVTDGFVESVAG
TH_0811|M.thermoresistible__bu AGELGRWVMRTACGEFAKWRAEGIGLDAVLRINVSPVQLVTDGFVASVAD
Mb1389c|M.bovis_AF2122/97 AGELDRWVLRRACNEFSEWQSAGLGHDALLRINVSAGQLVTGGFVDFVAD
Rv1354c|M.tuberculosis_H37Rv AGELDRWVLRRACNEFSEWQSAGLGHDALLRINVSAGQLVTGGFVDFVAD
MLBr_00397|M.leprae_Br4923 FAAIVTALLVGLAAGMVNGIVFAYGKIPSFVSTLGMLQVCRGITLMISDS
MAB_0124|M.abscessus_ATCC_1997 --------------------------------------------------
Mflv_4515|M.gilvum_PYR-GCK --------------------------------------------------
MAV_4962|M.avium_104 --------------------------------------------------
MSMEG_2196|M.smegmatis_MC2_155 IMKEFGLPRGSVCLEITESVVVQDIETTRTTLTGLHNVGVQVAIDDFGTG
Mvan_1967|M.vanbaalenii_PYR-1 IVDEFDLDEGSVCLEITESVVVQDIETTRVTLKGLKEAGVRIAIDDFGTG
TH_0811|M.thermoresistible__bu IIDEFGLDGSSVCLEITESVVVQDIKTTRITLEGLNEVGVQVAIDDFGTG
Mb1389c|M.bovis_AF2122/97 TIGQHGLDASSVCLEITENVVVQDLHTARATLARLKEVGVHIAIDDFGTG
Rv1354c|M.tuberculosis_H37Rv TIGQHGLDASSVCLEITENVVVQDLHTARATLARLKEVGVHIAIDDFGTG
MLBr_00397|M.leprae_Br4923 SAKPMPFHGILGAMGAMPWILIVCLFVTILAGILFQFTMFGRWVKAIG-G
MAB_0124|M.abscessus_ATCC_1997 --------------------------------------------------
Mflv_4515|M.gilvum_PYR-GCK --------------------------------------------------
MAV_4962|M.avium_104 --------------------------------------------------
MSMEG_2196|M.smegmatis_MC2_155 YSVLSLLKSLPVDTLKIDRSFVAELGSNPGDLPIVRAVIALAGAFGLQLV
Mvan_1967|M.vanbaalenii_PYR-1 YSALSHLKSLPVDTLKIDKSFVRELGSDPGDLAIVRAVIALAEAFGLELV
TH_0811|M.thermoresistible__bu YSVLSHLKTLPVDTLKIDKGFVQDLGNSPGDLAIVRAIIALAEAFGLQLV
Mb1389c|M.bovis_AF2122/97 YSAISLLQTLPIDTLKIDKTFVRQLGTNTSDLVIVRGIMTLAEGFQLDVV
Rv1354c|M.tuberculosis_H37Rv YSAISLLQTLPIDTLKIDKTFVRQLGTNTSDLVIVRGIMTLAEGFQLDVV
MLBr_00397|M.leprae_Br4923 NERVATLAGVPTRGIKVAIFAICGLTAGLGGIVLASRLGAGTPTAATGFE
MAB_0124|M.abscessus_ATCC_1997 --------------------------------------------------
Mflv_4515|M.gilvum_PYR-GCK --------------------------------------------------
MAV_4962|M.avium_104 --------------------------------------------------
MSMEG_2196|M.smegmatis_MC2_155 AEGVETERAALTLLRHGCYRAQGFLLSKPILGSEMQTLLAKGRVPVHFSA
Mvan_1967|M.vanbaalenii_PYR-1 AEGVETEAAAMTLLRHGCHRAQGFLLSRPIDGAAMKALLARGRVPVSFAK
TH_0811|M.thermoresistible__bu AEGVETEAAALTLLRHGCHRAQGFLLSRPIPSNEMRELLARGRIPMRFSA
Mb1389c|M.bovis_AF2122/97 AEGVETEAAARILLDQRCYRAQGFLFSRPVPGEAMRHMLSARRLPPTCIP
Rv1354c|M.tuberculosis_H37Rv AEGVETEAAARILLDQRCYRAQGFLFSRPVPGEAMRHMLSARRLPPTCIP
MLBr_00397|M.leprae_Br4923 IDVIAAVVIGGTPLTGGLGRLSGTLIGAIIISMLSNGMVFMGVGNAASQI
MAB_0124|M.abscessus_ATCC_1997 --------------------------------------------------
Mflv_4515|M.gilvum_PYR-GCK -----------------------
MAV_4962|M.avium_104 -----------------------
MSMEG_2196|M.smegmatis_MC2_155 APRM-------------------
Mvan_1967|M.vanbaalenii_PYR-1 S----------------------
TH_0811|M.thermoresistible__bu TP---------------------
Mb1389c|M.bovis_AF2122/97 ATDPALS----------------
Rv1354c|M.tuberculosis_H37Rv ATDPALS----------------
MLBr_00397|M.leprae_Br4923 IKGIMLAAAVFVFLQRRKIGIIK
MAB_0124|M.abscessus_ATCC_1997 -----------------------